LOCUS       NM_001135823            4364 bp    mRNA    linear   PRI 02-JUN-2024
DEFINITION  Homo sapiens sarcospan (SSPN), transcript variant 2, mRNA.
ACCESSION   NM_001135823
VERSION     NM_001135823.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Panzer,A.A., Regmi,S.D., Cormier,D., Danzo,M.T., Chen,I.D.,
            Winston,J.B., Hutchinson,A.K., Salm,D., Schulkey,C.E.,
            Cochran,R.S., Wilson,D.B. and Jay,P.Y.
  TITLE     Nkx2-5 and Sarcospan genetically interact in the development of the
            muscular ventricular septum of the heart
  JOURNAL   Sci Rep 7, 46438 (2017)
   PUBMED   28406175
  REMARK    GeneRIF: Data indicate that Sspn is an Nkx2-5 modifier gene.
            Complete loss of Sspn function does not cause heart defects but
            does increase the incidence of muscular ventricular septal defects
            (VSDs) in combination with an Nkx2-5 mutation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Gibbs,E.M., Marshall,J.L., Ma,E., Nguyen,T.M., Hong,G., Lam,J.S.,
            Spencer,M.J. and Crosbie-Watson,R.H.
  TITLE     High levels of sarcospan are well tolerated and act as a
            sarcolemmal stabilizer to address skeletal muscle and pulmonary
            dysfunction in DMD
  JOURNAL   Hum Mol Genet 25 (24), 5395-5406 (2016)
   PUBMED   27798107
  REMARK    GeneRIF: When human SSPN is expressed at three-fold levels in mdx
            mice, this increase in adhesion complex abundance improves muscle
            membrane stability, preventing many of the histopathological
            changes associated with Duchenne muscular dystrophy
REFERENCE   3  (bases 1 to 4364)
  AUTHORS   Chew,E.G.Y., Tan,J.H.J., Bahta,A.W., Ho,B.S., Liu,X., Lim,T.C.,
            Sia,Y.Y., Bigliardi,P.L., Heilmann,S., Wan,A.C.A., Nothen,M.M.,
            Philpott,M.P. and Hillmer,A.M.
  TITLE     Differential Expression between Human Dermal Papilla Cells from
            Balding and Non-Balding Scalps Reveals New Candidate Genes for
            Androgenetic Alopecia
  JOURNAL   J Invest Dermatol 136 (8), 1559-1567 (2016)
   PUBMED   27060448
  REMARK    GeneRIF: Our data suggest TWIST1 and SSPN to be the functionally
            relevant androgenetic alopecia genes at the 7p21.1 and 12p12.1 risk
            loci, respectively.
REFERENCE   4  (bases 1 to 4364)
  AUTHORS   Heid,I.M., Jackson,A.U., Randall,J.C., Winkler,T.W., Qi,L.,
            Steinthorsdottir,V., Thorleifsson,G., Zillikens,M.C.,
            Speliotes,E.K., Magi,R., Workalemahu,T., White,C.C.,
            Bouatia-Naji,N., Harris,T.B., Berndt,S.I., Ingelsson,E.,
            Willer,C.J., Weedon,M.N., Luan,J., Vedantam,S., Esko,T.,
            Kilpelainen,T.O., Kutalik,Z., Li,S., Monda,K.L., Dixon,A.L.,
            Holmes,C.C., Kaplan,L.M., Liang,L., Min,J.L., Moffatt,M.F.,
            Molony,C., Nicholson,G., Schadt,E.E., Zondervan,K.T., Feitosa,M.F.,
            Ferreira,T., Lango Allen,H., Weyant,R.J., Wheeler,E., Wood,A.R.,
            Estrada,K., Goddard,M.E., Lettre,G., Mangino,M., Nyholt,D.R.,
            Purcell,S., Smith,A.V., Visscher,P.M., Yang,J., McCarroll,S.A.,
            Nemesh,J., Voight,B.F., Absher,D., Amin,N., Aspelund,T., Coin,L.,
            Glazer,N.L., Hayward,C., Heard-Costa,N.L., Hottenga,J.J.,
            Johansson,A., Johnson,T., Kaakinen,M., Kapur,K., Ketkar,S.,
            Knowles,J.W., Kraft,P., Kraja,A.T., Lamina,C., Leitzmann,M.F.,
            McKnight,B., Morris,A.P., Ong,K.K., Perry,J.R., Peters,M.J.,
            Polasek,O., Prokopenko,I., Rayner,N.W., Ripatti,S., Rivadeneira,F.,
            Robertson,N.R., Sanna,S., Sovio,U., Surakka,I., Teumer,A., van
            Wingerden,S., Vitart,V., Zhao,J.H., Cavalcanti-Proenca,C.,
            Chines,P.S., Fisher,E., Kulzer,J.R., Lecoeur,C., Narisu,N.,
            Sandholt,C., Scott,L.J., Silander,K., Stark,K., Tammesoo,M.L.,
            Teslovich,T.M., Timpson,N.J., Watanabe,R.M., Welch,R.,
            Chasman,D.I., Cooper,M.N., Jansson,J.O., Kettunen,J.,
            Lawrence,R.W., Pellikka,N., Perola,M., Vandenput,L., Alavere,H.,
            Almgren,P., Atwood,L.D., Bennett,A.J., Biffar,R., Bonnycastle,L.L.,
            Bornstein,S.R., Buchanan,T.A., Campbell,H., Day,I.N., Dei,M.,
            Dorr,M., Elliott,P., Erdos,M.R., Eriksson,J.G., Freimer,N.B.,
            Fu,M., Gaget,S., Geus,E.J., Gjesing,A.P., Grallert,H., Grassler,J.,
            Groves,C.J., Guiducci,C., Hartikainen,A.L., Hassanali,N.,
            Havulinna,A.S., Herzig,K.H., Hicks,A.A., Hui,J., Igl,W.,
            Jousilahti,P., Jula,A., Kajantie,E., Kinnunen,L., Kolcic,I.,
            Koskinen,S., Kovacs,P., Kroemer,H.K., Krzelj,V., Kuusisto,J.,
            Kvaloy,K., Laitinen,J., Lantieri,O., Lathrop,G.M., Lokki,M.L.,
            Luben,R.N., Ludwig,B., McArdle,W.L., McCarthy,A., Morken,M.A.,
            Nelis,M., Neville,M.J., Pare,G., Parker,A.N., Peden,J.F.,
            Pichler,I., Pietilainen,K.H., Platou,C.G., Pouta,A.,
            Ridderstrale,M., Samani,N.J., Saramies,J., Sinisalo,J., Smit,J.H.,
            Strawbridge,R.J., Stringham,H.M., Swift,A.J., Teder-Laving,M.,
            Thomson,B., Usala,G., van Meurs,J.B., van Ommen,G.J., Vatin,V.,
            Volpato,C.B., Wallaschofski,H., Walters,G.B., Widen,E., Wild,S.H.,
            Willemsen,G., Witte,D.R., Zgaga,L., Zitting,P., Beilby,J.P.,
            James,A.L., Kahonen,M., Lehtimaki,T., Nieminen,M.S., Ohlsson,C.,
            Palmer,L.J., Raitakari,O., Ridker,P.M., Stumvoll,M., Tonjes,A.,
            Viikari,J., Balkau,B., Ben-Shlomo,Y., Bergman,R.N., Boeing,H.,
            Smith,G.D., Ebrahim,S., Froguel,P., Hansen,T., Hengstenberg,C.,
            Hveem,K., Isomaa,B., Jorgensen,T., Karpe,F., Khaw,K.T., Laakso,M.,
            Lawlor,D.A., Marre,M., Meitinger,T., Metspalu,A., Midthjell,K.,
            Pedersen,O., Salomaa,V., Schwarz,P.E., Tuomi,T., Tuomilehto,J.,
            Valle,T.T., Wareham,N.J., Arnold,A.M., Beckmann,J.S., Bergmann,S.,
            Boerwinkle,E., Boomsma,D.I., Caulfield,M.J., Collins,F.S.,
            Eiriksdottir,G., Gudnason,V., Gyllensten,U., Hamsten,A.,
            Hattersley,A.T., Hofman,A., Hu,F.B., Illig,T., Iribarren,C.,
            Jarvelin,M.R., Kao,W.H., Kaprio,J., Launer,L.J., Munroe,P.B.,
            Oostra,B., Penninx,B.W., Pramstaller,P.P., Psaty,B.M.,
            Quertermous,T., Rissanen,A., Rudan,I., Shuldiner,A.R., Soranzo,N.,
            Spector,T.D., Syvanen,A.C., Uda,M., Uitterlinden,A., Volzke,H.,
            Vollenweider,P., Wilson,J.F., Witteman,J.C., Wright,A.F.,
            Abecasis,G.R., Boehnke,M., Borecki,I.B., Deloukas,P.,
            Frayling,T.M., Groop,L.C., Haritunians,T., Hunter,D.J.,
            Kaplan,R.C., North,K.E., O'Connell,J.R., Peltonen,L.,
            Schlessinger,D., Strachan,D.P., Hirschhorn,J.N., Assimes,T.L.,
            Wichmann,H.E., Thorsteinsdottir,U., van Duijn,C.M., Stefansson,K.,
            Cupples,L.A., Loos,R.J., Barroso,I., McCarthy,M.I., Fox,C.S.,
            Mohlke,K.L. and Lindgren,C.M.
  CONSRTM   MAGIC
  TITLE     Meta-analysis identifies 13 new loci associated with waist-hip
            ratio and reveals sexual dimorphism in the genetic basis of fat
            distribution
  JOURNAL   Nat Genet 42 (11), 949-960 (2010)
   PUBMED   20935629
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
            Erratum:[Nat Genet. 2011 Nov;43(11):1164]
REFERENCE   5  (bases 1 to 4364)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   6  (bases 1 to 4364)
  AUTHORS   Yoshida,M., Hama,H., Ishikawa-Sakurai,M., Imamura,M., Mizuno,Y.,
            Araishi,K., Wakabayashi-Takai,E., Noguchi,S., Sasaoka,T. and
            Ozawa,E.
  TITLE     Biochemical evidence for association of dystrobrevin with the
            sarcoglycan-sarcospan complex as a basis for understanding
            sarcoglycanopathy
  JOURNAL   Hum Mol Genet 9 (7), 1033-1040 (2000)
   PUBMED   10767327
REFERENCE   7  (bases 1 to 4364)
  AUTHORS   Noguchi,S., Wakabayashi,E., Imamura,M., Yoshida,M. and Ozawa,E.
  TITLE     Formation of sarcoglycan complex with differentiation in cultured
            myocytes
  JOURNAL   Eur J Biochem 267 (3), 640-648 (2000)
   PUBMED   10651799
REFERENCE   8  (bases 1 to 4364)
  AUTHORS   Crosbie,R.H., Heighway,J., Venzke,D.P., Lee,J.C. and Campbell,K.P.
  TITLE     Sarcospan, the 25-kDa transmembrane component of the
            dystrophin-glycoprotein complex
  JOURNAL   J Biol Chem 272 (50), 31221-31224 (1997)
   PUBMED   9395445
REFERENCE   9  (bases 1 to 4364)
  AUTHORS   Heighway,J., Betticher,D.C., Hoban,P.R., Altermatt,H.J. and
            Cowen,R.
  TITLE     Coamplification in tumors of KRAS2, type 2 inositol 1,4,5
            triphosphate receptor gene, and a novel human gene, KRAG
  JOURNAL   Genomics 35 (1), 207-214 (1996)
   PUBMED   8661122
REFERENCE   10 (bases 1 to 4364)
  AUTHORS   Scott,A.F., Elizaga,A., Morrell,J., Bergen,A. and Penno,M.B.
  TITLE     Characterization of a gene coamplified with Ki-ras in Y1 murine
            adrenal carcinoma cells that codes for a putative membrane protein
  JOURNAL   Genomics 20 (2), 227-230 (1994)
   PUBMED   8020969
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA073350.1, AK092925.1,
            AC022509.21 and CA419006.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes a member of the dystrophin-glycoprotein
            complex (DGC). The DGC spans the sarcolemma and is comprised of
            dystrophin, syntrophin, alpha- and beta-dystroglycans and
            sarcoglycans. The DGC provides a structural link between the
            subsarcolemmal cytoskeleton and the extracellular matrix of muscle
            cells. Two alternatively spliced transcript variants that encode
            different protein isoforms have been described. [provided by
            RefSeq, Oct 2008].
            
            Transcript Variant: This variant (2) uses a different segment for
            its 5' UTR and lacks a 5' coding segment which results in the use
            of a downstream start codon, compared to variant 1. The resulting
            protein (isoform 2) is shorter when it is compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.28010.1,
                                           SRR1660805.131462.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-196               DA073350.1         1-196
            197-1589            AK092925.1         193-1585
            1590-4216           AC022509.21        153835-156461
            4217-4364           CA419006.1         1-148               c
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p12.1"
     gene            1..4364
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /note="sarcospan"
                     /db_xref="GeneID:8082"
                     /db_xref="HGNC:HGNC:11322"
                     /db_xref="MIM:601599"
     exon            1..196
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /inference="alignment:Splign:2.1.0"
     exon            197..283
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             227..649
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     microspan; kirsten-ras-associated protein; K-ras
                     oncogene-associated protein; nanospan; sarcospan (Kras
                     oncogene-associated gene)"
                     /codon_start=1
                     /product="sarcospan isoform 2"
                     /protein_id="NP_001129295.1"
                     /db_xref="CCDS:CCDS44850.1"
                     /db_xref="GeneID:8082"
                     /db_xref="HGNC:HGNC:11322"
                     /db_xref="MIM:601599"
                     /translation="MLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHY
                     SQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVC
                     GLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI"
     exon            284..4350
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4325..4330
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /note="hexamer: AATAAA"
     polyA_site      4350
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /note="major polyA site"
ORIGIN      
        1 actcgcacac gcgcgcactc gcagcctgtc tgaagtgcag aggttcaggc ggccaaggga
       61 cccgcgccgg tttccgcggg cgacctgcaa atcctgcccg gggcccggca ggcgctgcgt
      121 cccggcgcgt tggcagttgg cgacccccct cgaatccccc ctctgctgcg ggctccccgt
      181 ggcctttgga ggctcggtct gcttagtggc ctatcttggc ttgtttatgc tttgtgtctc
      241 atatcaggtt gacgaacgga catgtattca attttctatg aaactgttat actttctgct
      301 gagtgccctg ggcctgacgg tctgtgtgct ggccgtggcc tttgccgccc accactattc
      361 gcagctcaca cagtttacct gtgagaccac actcgactct tgccagtgca aactgccctc
      421 ctcggagccg ctcagcagga cctttgttta ccgggatgtg acggactgta ccagcgtcac
      481 tggcactttc aaactgttct tactcatcca gatgattctt aatttggtct gcggccttgt
      541 gtgcttgttg gcctgctttg tgatgtggaa acataggtac caggtcttct atgtgggtgt
      601 caggatatgc tccctcacgg cttccgaagg cccccagcaa aagatctaac attcttgctc
      661 aaagttgcga gagaaagtag cacatggagt agctgaggtt aaacaaacaa aaaaaaattt
      721 taaacaaaga aaggaaaaaa attgacaata aaagtcactc ttctaattga atatttttat
      781 atttttatga aacaaaagag catttcttca ggtttctatt gtattttttt taacattctt
      841 gcagagaaag caagatccaa attgattttg ggatattaaa agttaacaga acactgaaca
      901 aggaaagaat ggcatagatc tatctttaca gtctggagtt aattcctgtt aactcatttt
      961 atccattcct tacataatct tctttcctgt tagtccagtt tgatggtgtg aatggtgaat
     1021 ttcaggccca gttgctaaat tttgtggcat cttcctctag tccttcccac ctccagtcat
     1081 cagccccact ctgtcttgga gacaggcagg aggtggggga agagctgaat ctctttattt
     1141 tccctggtag agacatcttc aaggcatgaa atagcttaaa gagcagagta gaaacggaag
     1201 aggctttgca aaaggctaga taactaacaa cacctgggtt ggggcggcgg cctcttctct
     1261 tcagctccct tagcttggct ccgtaagtgg atcacttgcc aaatgcttta gatgattgcc
     1321 tctcaataat tgaaaggtgg tggtagttgt attctaaatg atgtagaagg tttaaaaata
     1381 attacattat gcttctattc tatcatctaa aacaaatcat taaaactaat ttctagctaa
     1441 ttgttaatta taattatgct cagaagtcta tttaatgagc tctgactgta cttacgctgc
     1501 actgtcggtg ttaagagaaa ttactctcac aagagcagag gcctgaagat tctttcttct
     1561 gaaagccaag caccacaagg aaaaaaaaaa ttattaatag ctcaggttaa aaacacccat
     1621 ttaaacaaaa acaagagcat ttgtaatagg aagtgtttat acaaacagca catttgtgat
     1681 atgttgaaaa gcatctctct tggcaaccaa tctatgtttg aggaagattg ggtaatgctg
     1741 atgtgttcca ttcatgaaac tgtatttgat acataatcct attattaatt cgtatgctta
     1801 gtcaacctag gaaatcaaaa taatgttttg aagttcttat ttgagcaata tggccttgac
     1861 ttggagggta gttttagttg ttttgttttt aagtgactgt ggtttaaagc acaaatgccc
     1921 caaggtgggg agacttctct ctgtgattat tgttgctatt aaattctgaa ctgtatccat
     1981 attttaagga aggagctaaa aatggaaatt catgaaacat aaatggtatc aagaacttta
     2041 tcagtatgct ttgttgaaag cagaaattaa gataataatt gagttcaatt cgcctctccg
     2101 cattgcctat tgatacactt tactaatcat gaaattctaa cctaaaagga aaacattttc
     2161 ctgcttgtct tagaagaaag tggaataatt ccactgattg tgataatggt ttcaatttct
     2221 acacaatata aatatccagt ataaaggaaa gcgttaagtc ggtaagctag aggattgtaa
     2281 atatctttta tgtcctctag ataaaacacc cgattaacag atgttaaacc ttttaatgtt
     2341 ttgatttgct ttaaaaatgg ccttcctaca cattagctcc agctaaaaag acacattgga
     2401 gagcttagag gataagtctc tggagcagaa tttatcacac acaaaagtta caccaacaga
     2461 ataccaagca gaatgatgag gacctgtaaa ataccttgtg ccctattaaa aaaaaaaaaa
     2521 aaaaaaaagc cagtaactga atccattttg atttttggtt gagtttccta cacaaagaag
     2581 aaaataactg agaatctgga atgttgtagt ccatccttta aagagtaaga aagtagcagt
     2641 taatgctagt aaccgtgaat taggcaccac tgaaagcaca tcccgaattt ctttaacaac
     2701 aacattttat agtgaacact acaagttttt atatttaaaa attaagactc tgtatatcct
     2761 taaggtgctc tatgctttac cagtaattca cagggtattt caaatggtag aatcatttta
     2821 gcttctgtgc ttcctttttc taaataatgc aacttgtaag agttgacatt gtaataagct
     2881 ttataatagt ataaccgtca ggagatatat atatatatat atacacatac acacacacac
     2941 acacatatat actatacata tataaaatgg ggatattact attgtatgat taaatcattc
     3001 ttaagtcccc aaggaaaaaa aatcataaac aaatagaaag aactaaacag aaaagaaaga
     3061 aagaaaagat ccctttgcta tgagctcgct gtatattgat aagtgtaaga attgtgttgc
     3121 atgaataacc gctttgggct ggatttgaag tattttgatg ttgtgttttg caaatattga
     3181 agttacaata atggcaacag aaaaggaaca gatacacagc tttacactta gcagaatgtt
     3241 acatttaaaa tctgacaagg cgccaagatg gtgactgtct cctctaaacc acgaaagagt
     3301 aagatttgtg caaccctcct cctcttccac ctccttcagg agaattaaat gaatcaagac
     3361 tttggaaaga cggggaagag ccgggacttg gcagtacttg aaacaggagg aatacaccag
     3421 cctaaatgta cagactttgt agccgagccc actcgatcgg tctgtgcctt cacgtgacca
     3481 ccatctgtgc ctccctcgct ccatccaaat ttgtgtaggc tgctccttgg agctatgcct
     3541 aaaatatagc tacaccagag ccctggaaac tgtagtcaag taacaggcct cactgttttt
     3601 tttctttgga ttaaaagtgt atatctctct actgaggggt ttccagcttt acacttctat
     3661 aggttgttct gacagtgaga acacattatg tacatgtttt ctattcatgt ccagggcatg
     3721 agaatcttaa tattcttata cttccctttt tgcatgcagt atgtctggaa tatgtttata
     3781 aaattttagc aaggggtgag atcaagggac aaaggtagag tggttattta aagacaatca
     3841 gaaatgaact taaaagctgc cctgcaaaac caatcctttc ctatcatgaa gagtaccttc
     3901 atattttcta gagattgtct ctgaaccgtt gctacatagc cataaatttc tgtaaatcat
     3961 gttggctatc agttcttgct attctcagag cactctatca tgtttttagg tgtatattgt
     4021 gtattcagga tagcattaaa aagaactgat ggcaaatgta ttgactcaca gtggaaacat
     4081 tcatttggat gaagaattag tggtttagcc tttgaaatga ataccttgaa tcatttcttc
     4141 tgattttcta gtagtttatt atctcaaatg attattatcc ccttcaaaaa tactggtctg
     4201 tactttggtg ttgtggtttc atttttctgg cattgatcaa atagcaaata caaaataaag
     4261 catttctaca tgtttgaggt ggttgttttt aaaggggata tgtgtaatgc agattttctt
     4321 tattaataaa attttcataa tctctgaaaa aaaaaaaaaa aaaa
//
