LOCUS       NM_001282131            1030 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens slingshot protein phosphatase 2 (SSH2), transcript
            variant 4, mRNA.
ACCESSION   NM_001282131
VERSION     NM_001282131.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Yang,D., Xiao,P., Li,Q., Fu,X., Pan,C., Lu,D., Wen,S., Xia,W.,
            He,D., Li,H., Fang,H., Shen,Y., Xu,Z., Lin,A., Wang,C., Yu,X.,
            Wu,J. and Sun,J.
  TITLE     Allosteric modulation of the catalytic VYD loop in Slingshot by its
            N-terminal domain underlies both Slingshot auto-inhibition and
            activation
  JOURNAL   J Biol Chem 293 (42), 16226-16241 (2018)
   PUBMED   30154244
  REMARK    GeneRIF: the N-terminal region of human Slingshot2 auto-inhibits
            its phosphatase activity in a noncompetitive manner
            Erratum:[J Biol Chem. 2019 Feb 22;294(8):2947. doi:
            10.1074/jbc.AAC119.007803.. PMID: 30796175]
REFERENCE   3  (bases 1 to 1030)
  AUTHORS   Cornelis,M.C., Byrne,E.M., Esko,T., Nalls,M.A., Ganna,A.,
            Paynter,N., Monda,K.L., Amin,N., Fischer,K., Renstrom,F.,
            Ngwa,J.S., Huikari,V., Cavadino,A., Nolte,I.M., Teumer,A., Yu,K.,
            Marques-Vidal,P., Rawal,R., Manichaikul,A., Wojczynski,M.K.,
            Vink,J.M., Zhao,J.H., Burlutsky,G., Lahti,J., Mikkila,V.,
            Lemaitre,R.N., Eriksson,J., Musani,S.K., Tanaka,T., Geller,F.,
            Luan,J., Hui,J., Magi,R., Dimitriou,M., Garcia,M.E., Ho,W.K.,
            Wright,M.J., Rose,L.M., Magnusson,P.K., Pedersen,N.L., Couper,D.,
            Oostra,B.A., Hofman,A., Ikram,M.A., Tiemeier,H.W.,
            Uitterlinden,A.G., van Rooij,F.J., Barroso,I., Johansson,I.,
            Xue,L., Kaakinen,M., Milani,L., Power,C., Snieder,H., Stolk,R.P.,
            Baumeister,S.E., Biffar,R., Gu,F., Bastardot,F., Kutalik,Z.,
            Jacobs,D.R. Jr., Forouhi,N.G., Mihailov,E., Lind,L., Lindgren,C.,
            Michaelsson,K., Morris,A., Jensen,M., Khaw,K.T., Luben,R.N.,
            Wang,J.J., Mannisto,S., Perala,M.M., Kahonen,M., Lehtimaki,T.,
            Viikari,J., Mozaffarian,D., Mukamal,K., Psaty,B.M., Doring,A.,
            Heath,A.C., Montgomery,G.W., Dahmen,N., Carithers,T., Tucker,K.L.,
            Ferrucci,L., Boyd,H.A., Melbye,M., Treur,J.L., Mellstrom,D.,
            Hottenga,J.J., Prokopenko,I., Tonjes,A., Deloukas,P., Kanoni,S.,
            Lorentzon,M., Houston,D.K., Liu,Y., Danesh,J., Rasheed,A.,
            Mason,M.A., Zonderman,A.B., Franke,L., Kristal,B.S.,
            Karjalainen,J., Reed,D.R., Westra,H.J., Evans,M.K., Saleheen,D.,
            Harris,T.B., Dedoussis,G., Curhan,G., Stumvoll,M., Beilby,J.,
            Pasquale,L.R., Feenstra,B., Bandinelli,S., Ordovas,J.M., Chan,A.T.,
            Peters,U., Ohlsson,C., Gieger,C., Martin,N.G., Waldenberger,M.,
            Siscovick,D.S., Raitakari,O., Eriksson,J.G., Mitchell,P.,
            Hunter,D.J., Kraft,P., Rimm,E.B., Boomsma,D.I., Borecki,I.B.,
            Loos,R.J., Wareham,N.J., Vollenweider,P., Caporaso,N., Grabe,H.J.,
            Neuhouser,M.L., Wolffenbuttel,B.H., Hu,F.B., Hypponen,E.,
            Jarvelin,M.R., Cupples,L.A., Franks,P.W., Ridker,P.M., van
            Duijn,C.M., Heiss,G., Metspalu,A., North,K.E., Ingelsson,E.,
            Nettleton,J.A., van Dam,R.M. and Chasman,D.I.
  CONSRTM   Coffee and Caffeine Genetics Consortium; International Parkinson's
            Disease Genomics Consortium (IPDGC); North American Brain
            Expression Consortium (NABEC); UK Brain Expression Consortium
            (UKBEC)
  TITLE     Genome-wide meta-analysis identifies six novel loci associated with
            habitual coffee consumption
  JOURNAL   Mol Psychiatry 20 (5), 647-656 (2015)
   PUBMED   25288136
  REMARK    Review article
REFERENCE   4  (bases 1 to 1030)
  AUTHORS   Xu,X., Gera,N., Li,H., Yun,M., Zhang,L., Wang,Y., Wang,Q.J. and
            Jin,T.
  TITLE     GPCR-mediated PLCbetagamma/PKCbeta/PKD signaling pathway regulates
            the cofilin phosphatase slingshot 2 in neutrophil chemotaxis
  JOURNAL   Mol Biol Cell 26 (5), 874-886 (2015)
   PUBMED   25568344
  REMARK    GeneRIF: Novel signaling pathway consisting of chemoattractant
            GPCR/Galphai protein, PLC, PKCbeta and PKDs that regulates
            SSH2/cofilin activity, F-actin polymerization and directional
            assembly of actin cytoskeleton in neutrophil chemotaxis was
            identified.
REFERENCE   5  (bases 1 to 1030)
  AUTHORS   Song,S.Y., Kang,M.R., Yoo,N.J. and Lee,S.H.
  TITLE     Mutational analysis of mononucleotide repeats in dual specificity
            tyrosine phosphatase genes in gastric and colon carcinomas with
            microsatellite instability
  JOURNAL   APMIS 118 (5), 389-393 (2010)
   PUBMED   20477815
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[APMIS. 2021 May;129(5):280. doi: 10.1111/apm.13126.. PMID:
            33864290]
REFERENCE   6  (bases 1 to 1030)
  AUTHORS   Kligys,K., Claiborne,J.N., DeBiase,P.J., Hopkinson,S.B., Wu,Y.,
            Mizuno,K. and Jones,J.C.
  TITLE     The slingshot family of phosphatases mediates Rac1 regulation of
            cofilin phosphorylation, laminin-332 organization, and motility
            behavior of keratinocytes
  JOURNAL   J Biol Chem 282 (44), 32520-32528 (2007)
   PUBMED   17848544
  REMARK    GeneRIF: Expression of phosphatase-dead versions of SSH proteins,
            SSH1, SSH2 & SSH3 results in phosphorylation/inactivation of
            cofilin, changes in cytoskeleton organization, loss of cell
            polarity, and assembly of aberrant arrays of laminin-332 in
            keratinocytes.
REFERENCE   7  (bases 1 to 1030)
  AUTHORS   Jung,S.K., Jeong,D.G., Yoon,T.S., Kim,J.H., Ryu,S.E. and Kim,S.J.
  TITLE     Crystal structure of human slingshot phosphatase 2
  JOURNAL   Proteins 68 (1), 408-412 (2007)
   PUBMED   17427953
REFERENCE   8  (bases 1 to 1030)
  AUTHORS   Ohta,Y., Kousaka,K., Nagata-Ohashi,K., Ohashi,K., Muramoto,A.,
            Shima,Y., Niwa,R., Uemura,T. and Mizuno,K.
  TITLE     Differential activities, subcellular distribution and tissue
            expression patterns of three members of Slingshot family
            phosphatases that dephosphorylate cofilin
  JOURNAL   Genes Cells 8 (10), 811-824 (2003)
   PUBMED   14531860
REFERENCE   9  (bases 1 to 1030)
  AUTHORS   Chan,S.Y., Chan,A.K., Cheung,B.P., Liang,Y. and Leung,M.P.
  TITLE     Identification of genes expressed during myocardial development
  JOURNAL   Chin Med J (Engl) 116 (9), 1329-1332 (2003)
   PUBMED   14527359
REFERENCE   10 (bases 1 to 1030)
  AUTHORS   Niwa,R., Nagata-Ohashi,K., Takeichi,M., Mizuno,K. and Uemura,T.
  TITLE     Control of actin reorganization by Slingshot, a family of
            phosphatases that dephosphorylate ADF/cofilin
  JOURNAL   Cell 108 (2), 233-246 (2002)
   PUBMED   11832213
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA861985.1, BC011636.2,
            BC008941.2 and AC104564.11.
            
            On May 31, 2019 this sequence version replaced NM_001282131.1.
            
            Summary: This gene encodes a protein tyrosine phosphatase that
            plays a key role in the regulation of actin filaments. The encoded
            protein dephosphorylates and activates cofilin, which promotes
            actin filament depolymerization. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (4) contains multiple differences
            in the UTRs and coding region, compared to variant 1, including the
            lack of multiple 5' and 3' coding exons. It represents use of an
            internal promoter and initiates translation at an alternate start
            codon. The encoded isoform (4) is shorter and has distinct N- and
            C- termini, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011636.2, CV030047.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                DA861985.1         1-42
            43-955              BC011636.2         1-913
            956-957             BC008941.2         932-933
            958-1030            AC104564.11        47599-47671
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q11.2"
     gene            1..1030
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /note="slingshot protein phosphatase 2"
                     /db_xref="GeneID:85464"
                     /db_xref="HGNC:HGNC:30580"
                     /db_xref="MIM:606779"
     exon            1..283
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..764
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /note="isoform 4 is encoded by transcript variant 4;
                     protein phosphatase Slingshot homolog 2; SSH-like protein
                     2"
                     /codon_start=1
                     /product="protein phosphatase Slingshot homolog 2 isoform
                     4"
                     /protein_id="NP_001269060.1"
                     /db_xref="CCDS:CCDS92286.1"
                     /db_xref="GeneID:85464"
                     /db_xref="HGNC:HGNC:30580"
                     /db_xref="MIM:606779"
                     /translation="MTLSTLARKRKAPLACTCSLGGPDMIPYFSANAVISQNAINQLI
                     SESFLTVKGAALFLPRGNGSSTPRISHRRNKHAGDLQQHLQAMFILLRPEDNIRLAVR
                     LESTYQNRTRYMVVVSTNGRQDTEESIVLGMDFSSNDSSTCTMGLVLPLWSDTLIHLD
                     GDGGFSVSTDNRVHIFKPVSVQAMWVDRDSRNKHCDVLLVEE"
     exon            284..387
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            388..452
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            453..574
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            575..643
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            644..709
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            710..1030
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      939..944
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /note="hexamer: AACAAA"
     polyA_site      957
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /note="major polyA site"
     regulatory      1005..1010
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /note="hexamer: ATTAAA"
     polyA_site      1030
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
ORIGIN      
        1 agtccggact ctaccgccga gccctggggc ttcggcggct gcctccgcgc gacccggcgg
       61 gcccgcctcc gccgccgggt gcacgcgcag cctgcgcctc gtgccggagc agagcagagg
      121 ccaggatgcg gagttgtgca ctttgagccg gccccatgac cctgagcacg ttggcccgca
      181 agaggaaggc gcccctcgct tgcacctgca gcctcggtgg ccccgacatg attccttact
      241 tctccgccaa cgcggtcatc tcgcagaacg ccatcaacca gctcatcagc gagagctttc
      301 taactgtcaa aggtgctgcc ctttttctac cacggggaaa tggctcatcc acaccaagaa
      361 tcagccacag acggaacaag catgcaggcg atctccaaca gcatctccaa gcaatgttca
      421 ttttactccg cccagaagac aacatcaggc tggctgtaag actggaaagt acttaccaga
      481 atcgaacacg ctatatggta gtggtttcaa ctaatggtag acaagacact gaagaaagca
      541 tcgtcctagg aatggatttc tcctctaatg acagtagcac ttgtaccatg ggcttagttt
      601 tgcctctctg gagcgacacg ctaattcatt tggatggtga tggtgggttc agtgtatcga
      661 cggataacag agttcacata ttcaaacctg tatctgtgca ggcaatgtgg gttgacaggg
      721 attcaaggaa caaacactgt gatgtactat tggtggaaga atgaactgga gcagcctttc
      781 tggagagtga tttgccaata tgccttatca ttttgcatga tctttgtcct agtaactcta
      841 tttctatgga tttactctaa gtttgtaaac atggatgtgt gcaaagattt tagctctaag
      901 aatgtttgtc agtgttctaa taatagcaaa aaataaaaaa caaatgattg aaaaatagaa
      961 ttagtaaatt atggtacatc tatatcatta aatattatgc aaccattaaa aatgatccag
     1021 ttgaaaaata
//
