LOCUS       NM_001282129            9484 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens slingshot protein phosphatase 2 (SSH2), transcript
            variant 1, mRNA.
ACCESSION   NM_001282129
VERSION     NM_001282129.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Yang,D., Xiao,P., Li,Q., Fu,X., Pan,C., Lu,D., Wen,S., Xia,W.,
            He,D., Li,H., Fang,H., Shen,Y., Xu,Z., Lin,A., Wang,C., Yu,X.,
            Wu,J. and Sun,J.
  TITLE     Allosteric modulation of the catalytic VYD loop in Slingshot by its
            N-terminal domain underlies both Slingshot auto-inhibition and
            activation
  JOURNAL   J Biol Chem 293 (42), 16226-16241 (2018)
   PUBMED   30154244
  REMARK    GeneRIF: the N-terminal region of human Slingshot2 auto-inhibits
            its phosphatase activity in a noncompetitive manner
            Erratum:[J Biol Chem. 2019 Feb 22;294(8):2947. doi:
            10.1074/jbc.AAC119.007803.. PMID: 30796175]
REFERENCE   3  (bases 1 to 9484)
  AUTHORS   Cornelis,M.C., Byrne,E.M., Esko,T., Nalls,M.A., Ganna,A.,
            Paynter,N., Monda,K.L., Amin,N., Fischer,K., Renstrom,F.,
            Ngwa,J.S., Huikari,V., Cavadino,A., Nolte,I.M., Teumer,A., Yu,K.,
            Marques-Vidal,P., Rawal,R., Manichaikul,A., Wojczynski,M.K.,
            Vink,J.M., Zhao,J.H., Burlutsky,G., Lahti,J., Mikkila,V.,
            Lemaitre,R.N., Eriksson,J., Musani,S.K., Tanaka,T., Geller,F.,
            Luan,J., Hui,J., Magi,R., Dimitriou,M., Garcia,M.E., Ho,W.K.,
            Wright,M.J., Rose,L.M., Magnusson,P.K., Pedersen,N.L., Couper,D.,
            Oostra,B.A., Hofman,A., Ikram,M.A., Tiemeier,H.W.,
            Uitterlinden,A.G., van Rooij,F.J., Barroso,I., Johansson,I.,
            Xue,L., Kaakinen,M., Milani,L., Power,C., Snieder,H., Stolk,R.P.,
            Baumeister,S.E., Biffar,R., Gu,F., Bastardot,F., Kutalik,Z.,
            Jacobs,D.R. Jr., Forouhi,N.G., Mihailov,E., Lind,L., Lindgren,C.,
            Michaelsson,K., Morris,A., Jensen,M., Khaw,K.T., Luben,R.N.,
            Wang,J.J., Mannisto,S., Perala,M.M., Kahonen,M., Lehtimaki,T.,
            Viikari,J., Mozaffarian,D., Mukamal,K., Psaty,B.M., Doring,A.,
            Heath,A.C., Montgomery,G.W., Dahmen,N., Carithers,T., Tucker,K.L.,
            Ferrucci,L., Boyd,H.A., Melbye,M., Treur,J.L., Mellstrom,D.,
            Hottenga,J.J., Prokopenko,I., Tonjes,A., Deloukas,P., Kanoni,S.,
            Lorentzon,M., Houston,D.K., Liu,Y., Danesh,J., Rasheed,A.,
            Mason,M.A., Zonderman,A.B., Franke,L., Kristal,B.S.,
            Karjalainen,J., Reed,D.R., Westra,H.J., Evans,M.K., Saleheen,D.,
            Harris,T.B., Dedoussis,G., Curhan,G., Stumvoll,M., Beilby,J.,
            Pasquale,L.R., Feenstra,B., Bandinelli,S., Ordovas,J.M., Chan,A.T.,
            Peters,U., Ohlsson,C., Gieger,C., Martin,N.G., Waldenberger,M.,
            Siscovick,D.S., Raitakari,O., Eriksson,J.G., Mitchell,P.,
            Hunter,D.J., Kraft,P., Rimm,E.B., Boomsma,D.I., Borecki,I.B.,
            Loos,R.J., Wareham,N.J., Vollenweider,P., Caporaso,N., Grabe,H.J.,
            Neuhouser,M.L., Wolffenbuttel,B.H., Hu,F.B., Hypponen,E.,
            Jarvelin,M.R., Cupples,L.A., Franks,P.W., Ridker,P.M., van
            Duijn,C.M., Heiss,G., Metspalu,A., North,K.E., Ingelsson,E.,
            Nettleton,J.A., van Dam,R.M. and Chasman,D.I.
  CONSRTM   Coffee and Caffeine Genetics Consortium; International Parkinson's
            Disease Genomics Consortium (IPDGC); North American Brain
            Expression Consortium (NABEC); UK Brain Expression Consortium
            (UKBEC)
  TITLE     Genome-wide meta-analysis identifies six novel loci associated with
            habitual coffee consumption
  JOURNAL   Mol Psychiatry 20 (5), 647-656 (2015)
   PUBMED   25288136
  REMARK    Review article
REFERENCE   4  (bases 1 to 9484)
  AUTHORS   Xu,X., Gera,N., Li,H., Yun,M., Zhang,L., Wang,Y., Wang,Q.J. and
            Jin,T.
  TITLE     GPCR-mediated PLCbetagamma/PKCbeta/PKD signaling pathway regulates
            the cofilin phosphatase slingshot 2 in neutrophil chemotaxis
  JOURNAL   Mol Biol Cell 26 (5), 874-886 (2015)
   PUBMED   25568344
  REMARK    GeneRIF: Novel signaling pathway consisting of chemoattractant
            GPCR/Galphai protein, PLC, PKCbeta and PKDs that regulates
            SSH2/cofilin activity, F-actin polymerization and directional
            assembly of actin cytoskeleton in neutrophil chemotaxis was
            identified.
REFERENCE   5  (bases 1 to 9484)
  AUTHORS   Song,S.Y., Kang,M.R., Yoo,N.J. and Lee,S.H.
  TITLE     Mutational analysis of mononucleotide repeats in dual specificity
            tyrosine phosphatase genes in gastric and colon carcinomas with
            microsatellite instability
  JOURNAL   APMIS 118 (5), 389-393 (2010)
   PUBMED   20477815
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[APMIS. 2021 May;129(5):280. doi: 10.1111/apm.13126.. PMID:
            33864290]
REFERENCE   6  (bases 1 to 9484)
  AUTHORS   Kligys,K., Claiborne,J.N., DeBiase,P.J., Hopkinson,S.B., Wu,Y.,
            Mizuno,K. and Jones,J.C.
  TITLE     The slingshot family of phosphatases mediates Rac1 regulation of
            cofilin phosphorylation, laminin-332 organization, and motility
            behavior of keratinocytes
  JOURNAL   J Biol Chem 282 (44), 32520-32528 (2007)
   PUBMED   17848544
  REMARK    GeneRIF: Expression of phosphatase-dead versions of SSH proteins,
            SSH1, SSH2 & SSH3 results in phosphorylation/inactivation of
            cofilin, changes in cytoskeleton organization, loss of cell
            polarity, and assembly of aberrant arrays of laminin-332 in
            keratinocytes.
REFERENCE   7  (bases 1 to 9484)
  AUTHORS   Jung,S.K., Jeong,D.G., Yoon,T.S., Kim,J.H., Ryu,S.E. and Kim,S.J.
  TITLE     Crystal structure of human slingshot phosphatase 2
  JOURNAL   Proteins 68 (1), 408-412 (2007)
   PUBMED   17427953
REFERENCE   8  (bases 1 to 9484)
  AUTHORS   Ohta,Y., Kousaka,K., Nagata-Ohashi,K., Ohashi,K., Muramoto,A.,
            Shima,Y., Niwa,R., Uemura,T. and Mizuno,K.
  TITLE     Differential activities, subcellular distribution and tissue
            expression patterns of three members of Slingshot family
            phosphatases that dephosphorylate cofilin
  JOURNAL   Genes Cells 8 (10), 811-824 (2003)
   PUBMED   14531860
REFERENCE   9  (bases 1 to 9484)
  AUTHORS   Chan,S.Y., Chan,A.K., Cheung,B.P., Liang,Y. and Leung,M.P.
  TITLE     Identification of genes expressed during myocardial development
  JOURNAL   Chin Med J (Engl) 116 (9), 1329-1332 (2003)
   PUBMED   14527359
REFERENCE   10 (bases 1 to 9484)
  AUTHORS   Niwa,R., Nagata-Ohashi,K., Takeichi,M., Mizuno,K. and Uemura,T.
  TITLE     Control of actin reorganization by Slingshot, a family of
            phosphatases that dephosphorylate ADF/cofilin
  JOURNAL   Cell 108 (2), 233-246 (2002)
   PUBMED   11832213
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC104982.7, DA749059.1,
            AK296413.1, AB099290.1 and AI609950.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001282129.1.
            
            Summary: This gene encodes a protein tyrosine phosphatase that
            plays a key role in the regulation of actin filaments. The encoded
            protein dephosphorylates and activates cofilin, which promotes
            actin filament depolymerization. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK296413.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000540801.6/ ENSP00000444743.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AC104982.7         97982-98000         c
            20-78               DA749059.1         20-78
            79-135              DA749059.1         80-136
            136-1111            AK296413.1         1-976
            1112-9475           AB099290.1         803-9166
            9476-9484           AI609950.1         10-18               c
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q11.2"
     gene            1..9484
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /note="slingshot protein phosphatase 2"
                     /db_xref="GeneID:85464"
                     /db_xref="HGNC:HGNC:30580"
                     /db_xref="MIM:606779"
     exon            1..291
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     CDS             229..4581
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein phosphatase Slingshot homolog 2; SSH-like protein
                     2"
                     /codon_start=1
                     /product="protein phosphatase Slingshot homolog 2 isoform
                     1"
                     /protein_id="NP_001269058.1"
                     /db_xref="CCDS:CCDS74024.1"
                     /db_xref="GeneID:85464"
                     /db_xref="HGNC:HGNC:30580"
                     /db_xref="MIM:606779"
                     /translation="MALVTVQRSPTPSTTSSPCASSSHLEDSESAALLCCECEESEIF
                     TDSNEADSGEEECRSQPRSISESFLTVKGAALFLPRGNGSSTPRISHRRNKHAGDLQQ
                     HLQAMFILLRPEDNIRLAVRLESTYQNRTRYMVVVSTNGRQDTEESIVLGMDFSSNDS
                     STCTMGLVLPLWSDTLIHLDGDGGFSVSTDNRVHIFKPVSVQAMWSALQSLHKACEVA
                     RAHNYYPGSLFLTWVSYYESHINSDQSSVNEWNAMQDVQSHRPDSPALFTDIPTERER
                     TERLIKTKLREIMMQKDLENITSKEIRTELEMQMVCNLREFKEFIDNEMIVILGQMDS
                     PTQIFEHVFLGSEWNASNLEDLQNRGVRYILNVTREIDNFFPGVFEYHNIRVYDEEAT
                     DLLAYWNDTYKFISKAKKHGSKCLVHCKMGVSRSASTVIAYAMKEYGWNLDRAYDYVK
                     ERRTVTKPNPSFMRQLEEYQGILLASKQRHNKLWRSHSDSDLSDHHEPICKPGLELNK
                     KDITTSADQIAEVKTMESHPPIPPVFVEHMVPQDANQKGLCTKERMICLEFTSREFHA
                     GQIEDELNLNDINGCSSGCCLNESKFPLDNCHASKALIQPGHVPEMANKFPDLTVEDL
                     ETDALKADMNVHLLPMEELTSPLKDPPMSPDPESPSPQPSCQTEISDFSTDRIDFFSA
                     LEKFVELSQETRSRSFSHSRMEELGGGRNESCRLSVVEVAPSKVTADDQRSSSLSNTP
                     HASEESSMDEEQSKAISELVSPDIFMQSHSENAISVKEIVTEIESISQGVGQIQLKGD
                     ILPNPCHTPKKNSIHELLLERAQTPENKPGHMEQDEDSCTAQPELAKDSGMCNPEGCL
                     TTHSSIADLEEGEPAEGEQELQGSGMHPGAKWYPGSVRRATLEFEERLRQEQEHHGAA
                     PTCTSLSTRKNSKNDSSVADLAPKGKSDEAPPEHSFVLKEPEMSKGKGKYSGSEAGSL
                     SHSEQNATVPAPRVLEFDHLPDPQEGPGSDTGTQQEGVLKDLRTVIPYQESETQAVPL
                     PLPKRVEIIEYTHIVTSPNHTGPGSEIATSEKSGEQGLRKVNMEKSVTVLCTLDENLN
                     RTLDPNQVSLHPQVLPLPHSSSPEHNRPTDHPTSILSSPEDRGSSLSTALETAAPFVS
                     HTTHLLSASLDYLHPQTMVHLEGFTEQSSTTDEPSAEQVSWEESQESPLSSGSEVPYK
                     DSQLSSADLSLISKLGDNTGELQEKMDPLPVACRLPHSSSSENIKSLSHSPGVVKERA
                     KEIESRVVFQAGLTKPSQMRRSASLAKLGYLDLCKDCLPEREPASCESPHLKLLQPFL
                     RTDSGMHAMEDQESLENPGAPHNPEPTKSFVEQLTTTECIVQSKPVERPLVQYAKEFG
                     SSQQYLLPRAGLELTSSEGGLPVLQTQGLQCACPAPGLAVAPRQQHGRTHPLRRLKKA
                     NDKKRTTNPFYNTM"
     exon            292..372
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            373..416
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            417..520
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            521..585
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            586..707
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            708..776
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            777..842
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            843..1037
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1131
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1260
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1307
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1454
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1655
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..2490
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     exon            2491..9484
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9459..9464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /note="hexamer: AATAAA"
     polyA_site      9484
                     /gene="SSH2"
                     /gene_synonym="SSH-2; SSH-2L"
                     /note="major polyA site"
ORIGIN      
        1 attgtgcccc gcagctgcgg aaccgcccgc ccgccgcccg ttcggagcgt cagtcggcga
       61 cagtctggtg gtgggtgcgg agtctgcggc cgttcccgcg gcctcctcct cctccccgtt
      121 cccttcaccc ccaccccgca cccctttccc catcccggct ccgtcaccct cccgtccccc
      181 acactcagga caagaatgcc ctgcccggaa caacccagca gcgcctagat ggctttggtc
      241 acggtccagc ggtcacctac ccccagcacc acctccagcc cctgcgcctc gagctctcat
      301 ttggaagaca gtgaatcagc agcattactt tgctgtgaat gtgaagaatc agaaatcttt
      361 actgattcca atgaggcaga cagtggggag gaagaatgcc ggtcacagcc caggagcatc
      421 agcgagagct ttctaactgt caaaggtgct gccctttttc taccacgggg aaatggctca
      481 tccacaccaa gaatcagcca cagacggaac aagcatgcag gcgatctcca acagcatctc
      541 caagcaatgt tcattttact ccgcccagaa gacaacatca ggctggctgt aagactggaa
      601 agtacttacc agaatcgaac acgctatatg gtagtggttt caactaatgg tagacaagac
      661 actgaagaaa gcatcgtcct aggaatggat ttctcctcta atgacagtag cacttgtacc
      721 atgggcttag ttttgcctct ctggagcgac acgctaattc atttggatgg tgatggtggg
      781 ttcagtgtat cgacggataa cagagttcac atattcaaac ctgtatctgt gcaggcaatg
      841 tggtctgcac tacagagctt acacaaggct tgtgaagtcg ccagagcgca taactactac
      901 ccaggcagcc tatttctcac ttgggtgagt tattatgaga gccatatcaa ctcagatcaa
      961 tcctcagtca atgaatggaa tgcaatgcaa gatgtacagt cccaccggcc cgactctcca
     1021 gctctcttca ccgacatacc tactgaacgt gaacgaacag aaaggctaat taaaaccaaa
     1081 ttaagggaga tcatgatgca gaaggatttg gagaatatta catccaaaga gataagaaca
     1141 gagttggaaa tgcaaatggt gtgcaacttg cgggaattca aggaatttat agacaatgaa
     1201 atgatagtga tccttggtca aatggatagc cctacacaga tatttgagca tgtgttcctg
     1261 ggctcagaat ggaatgcctc caacttagag gacttacaga accgaggggt acggtatatc
     1321 ttgaatgtca ctcgagagat agataacttc ttcccaggag tctttgagta tcataacatt
     1381 cgggtatatg atgaagaggc aacggatctc ctggcgtact ggaatgacac ttacaaattc
     1441 atctctaaag caaagaaaca tggatctaaa tgccttgtgc actgcaaaat gggggtgagt
     1501 cgctcagcct ccaccgtgat tgcctatgca atgaaggaat atggctggaa tctggaccga
     1561 gcctatgact atgtgaaaga aagacgaacg gtaaccaagc ccaacccaag cttcatgaga
     1621 caactggaag agtatcaggg gatcttgctg gcaagcaaac agcggcataa caaactatgg
     1681 agatctcatt cagatagtga cctctcagac caccacgaac ccatctgcaa acctgggcta
     1741 gaactcaaca agaaggatat caccacctca gcagaccaga ttgctgaggt gaagaccatg
     1801 gagagtcacc cacccatacc tcctgtcttt gtggaacata tggtcccaca agatgcaaat
     1861 cagaaaggcc tgtgtaccaa agaaagaatg atctgcttgg agtttacttc tagggaattt
     1921 catgctggac agattgagga tgaattaaac ttaaatgaca tcaatggatg ctcatcaggg
     1981 tgttgtctga atgaatcaaa atttcctctt gacaattgcc atgcatccaa agccttaatt
     2041 cagcctggac atgtcccaga aatggccaac aagtttccag acttaacagt ggaagatttg
     2101 gagacagatg cactgaaagc agacatgaat gtccacctac tgcctatgga agaattgaca
     2161 tctccactga aagacccccc catgtcccct gatcctgagt caccaagccc ccaacccagt
     2221 tgccagactg aaatctcaga tttcagtaca gatcgcattg acttttttag tgccctagag
     2281 aagtttgtgg agctctccca agaaacccgg tcacgatctt tttcccattc aaggatggag
     2341 gaactgggtg gaggaaggaa tgagagctgt cgactgtcag tggtagaagt agccccttcc
     2401 aaagtgacag ctgatgacca gagaagcagc tctttgagta atactcccca tgcatcagaa
     2461 gaatcttcaa tggatgagga acagtcaaag gcaatttcag aactggtcag cccagacatc
     2521 ttcatgcagt ctcactcgga aaatgcaatt tcagtcaaag aaattgtcac tgaaattgag
     2581 tccatcagtc aaggagttgg gcagattcaa ctgaaaggag acatcttacc caacccatgc
     2641 catacaccaa agaagaacag catccatgag ctgctccttg agagggccca gactccagag
     2701 aacaaacctg gacatatgga gcaagatgag gactcctgca cagcccagcc tgaactagcc
     2761 aaagactcag ggatgtgcaa cccagaaggc tgcctaacca cacactcatc tatagcagac
     2821 ttggaagaag gggaaccagc tgagggggaa caagagctcc agggctcagg gatgcaccca
     2881 ggtgccaagt ggtaccctgg gtctgtgagg cgagccacct tggagttcga agagcgctta
     2941 cggcaggagc aagagcatca tggtgctgcc ccaacatgta cctcattgtc cactcgtaag
     3001 aattcaaaga atgattcttc tgtggcagac ctagcaccaa aagggaaaag tgatgaagcc
     3061 cccccagaac attcatttgt cctcaaggaa ccagaaatga gcaaaggcaa agggaaatac
     3121 agtgggtctg aggctggctc actgtcccat tctgagcaga atgccactgt tccagctccc
     3181 agggtgctgg agtttgacca cttgccagat cctcaggagg gcccagggtc agatactgga
     3241 acacagcagg aaggagtcct gaaggatctg aggactgtga ttccatacca ggagtctgaa
     3301 acacaagcag tccctcttcc ccttcccaag agggtagaaa tcattgaata tacccacata
     3361 gttacatcac ccaatcacac tgggccaggg agtgaaatag ccaccagtga gaagagcgga
     3421 gagcaagggc tgaggaaagt gaacatggaa aaatctgtca ctgtgctctg cacactggat
     3481 gaaaatctaa acaggactct ggaccccaac caggtttctc tgcaccccca agtgctacct
     3541 ctgcctcatt cttcctcccc tgagcacaac agacccactg accatccaac ctccatcctg
     3601 agtagccctg aagacagagg cagcagcctg tccacagccc tggagacagc agcacctttt
     3661 gtcagtcata caacccattt actgtctgcc agtttggatt acctgcatcc ccagactatg
     3721 gttcacctgg agggcttcac agagcagagc agcactacag atgagccctc tgcagaacag
     3781 gttagctggg aagaaagtca ggagagccct ctctccagtg gcagtgaggt gccatataag
     3841 gactcccagc taagtagcgc agacctaagt ttaattagca aacttggtga caacactggg
     3901 gagttacagg agaaaatgga cccattgcct gtagcctgtc gactcccaca tagctctagt
     3961 agtgaaaaca taaagagtct cagccacagc cccggtgtgg tgaaggagcg tgctaaagaa
     4021 atcgagtctc gagtggtttt ccaggcaggg ctcaccaaac catcccaaat gaggcgctca
     4081 gcttctctcg ccaaattagg ttacttggac ctctgtaaag actgcttacc agagagggag
     4141 cctgcctcct gtgaatcccc tcatctcaaa ctgcttcagc ctttcctcag aacagactca
     4201 ggcatgcacg cgatggagga ccaagagtcc ctagaaaacc caggtgcccc ccacaaccca
     4261 gagcccacca agtcttttgt agaacaactc acaacaacag agtgtattgt gcagagcaag
     4321 ccagtggaga ggccccttgt gcagtatgcc aaagaatttg gttctagtca gcagtatttg
     4381 ctccccaggg caggacttga attgactagt tctgaaggag gccttcccgt gctacagacc
     4441 cagggactgc agtgtgcatg cccagctcca gggctggccg tggcaccccg tcagcaacac
     4501 ggcagaactc acccccttag gagactgaaa aaggcaaatg acaaaaaacg gacaaccaac
     4561 cccttctata ataccatgtg attctgagcc tacacatgtg actttctaga agaaatgttt
     4621 gtaaaagggg caggtgtaat atgtaaggaa catgcacttt attggttaat tttataatat
     4681 tttggtcatt ttactgtttc tggtgcatgc agggtttggg tgtttttcag tgtgtatgtg
     4741 tgtgtatatg taaggggaaa gagagattga tctggatggc aagaccgttt atcatttttt
     4801 atttaaaaaa atcaaacctc aaaaaagtca ttttcagaga acacctttat caaaggcaaa
     4861 ttgctgtttt tcagtcagct gccacctgct tctcattttg ccctctgaga aaaggcatgg
     4921 tttcttaatt gagggaagga agcagattcg gaggggtgga gataaagctg gggattggag
     4981 atgttcccct gcctttgtga tgatggtttg gtttccagac ctgaggcatc gaatgggcta
     5041 aaggtcagcc agaagtcaga gggctctcag ccttcatcca gccgcagccc tttagagttc
     5101 ctgaaggagg caggttccgc tttgctttaa aagagggtct tccggtgttc cagagtgtat
     5161 cgttacagta agacatgata atggccggct ctgcaaattt atcatatagt ttttttccca
     5221 agaaaattat ttttgaaatt aaaacaggac cccaaatgaa atccaagtgg tcttctgtag
     5281 aggaaagcat gataagagga ggaagacata aagttggggg tggagaggct gctgcccaga
     5341 aactgctggg ttggtgggat ccacacagat gggcatcttg gctctccctg ccagaaaaat
     5401 gtgctctgca gtgtcctccc aactgaaaac aacagtgaca tgtttgaaaa gcagcaatcg
     5461 aaagtcagtg tgttcttgta aaatgaatat gtatgtaggg ttttaaccct tccattactt
     5521 gaataattct ttgggccttc tgagtttaat ggccatattt tcttccagtt tattttcctt
     5581 ccccatgctg ttgtccccat gctgcctatt tttttaaatt tcatagatta tatttgaatt
     5641 gttacatatg tttaacatcc attgaatgca acgttttatt tttggtatta tcactgttaa
     5701 cacttttttt tccttttcct ggaaggtttg tcatttctct aaagtttgta cacagtattt
     5761 atgtacataa tattgtcact gtcttagtgt cttgttcatg ttgtagggta attttgattt
     5821 attcttttta agaaggagta gagttgcaac tggaaaacac agacaccaga aatgaaagtg
     5881 ttgggtgttg ataggaatct gtacaatatg ttgatggtgg ctgtagttgt ttaaatggag
     5941 acttccaatc accagctcct gccaaattat gcattagtaa ctttttcctc agagacaccc
     6001 ctaaaggctc ccattaggtc acagcgtgac tgaaagctga tttctctgtg agactgaaat
     6061 cccagaacat tgtttgccag tatctcaacc acagccagga agtatatgtg agttgcccag
     6121 agtggaagct cctagtagag gctgaaggcc acaggggagg cctcagccag gggcaagtgc
     6181 ccacagtgac tccatttagg atgctgtcgt tttgctactg ttgtggagat ccttaacgca
     6241 gcagcgcctg tcataaacaa gcctcttgta gcttcttaca gaaaacagat ttagtctagc
     6301 atgaaacacc tctgcatctc attgaaactg aagaggcttg cagatttgct ttcgttgatg
     6361 tccacgttac tgaggattga ctgagtatag actggagaca ggtttttggc ttatcacagc
     6421 actgaggaaa atacaaatgc acagactgtc agcatgatgt cagaggccag agtttggtgg
     6481 gggcagatgg cagggcgacc caaaagatgc agaaaaggta gactggggcc actgggtctt
     6541 aagaggagct gtgaagttgt gtggaagcca aggctgggtc tgacatccct gccaggaagc
     6601 aggttggaca gaaaccactt gctaaaagcg tctctatggt gcagtgatta tgtgtaccct
     6661 gtgctgttgc tcagtagtct ccttgaatgt tcaagtatct catccactca gttttgtgat
     6721 tcctggatat ttgtgtgttg taaagtgttt tgttaggtgg tatgaaccaa tgtgaggagg
     6781 cttgagacag ggttatggat ttccagagct cttccatgta gaataccctg ccttcttgaa
     6841 gcagttccac caccaatatg atggggaagg gcaggaactt atggcagggt ggggagaggg
     6901 tttgcctgga agttaagaga gtggcatcca cttgtggtaa ccccttacga tgatgacatc
     6961 ccacacacta agaggtacaa gcaggaccaa agttttggac tgttccctct tctcccctcc
     7021 actgtgttta atgttcactc actgcattaa ataacaacaa agatgggaca aaacccacta
     7081 atactgtggg gaaaaaaaat tttcaagagc agaggaaagt ctttctgcct ttttataatt
     7141 acttttttta tcatttaatt tacctgccta aataaacatc ttctacagaa tatctgtcat
     7201 tcaaccatgc caaggagttt gtgccagcct tttcagactc ccagaattgg gtgttcctgg
     7261 gttatttaca tagctgagca gtgtccaggg gaggtgcgca gtgaactgtc aaggactttc
     7321 cagactgtct tctcttcaga cttttataat ctccagtggc atctttccct tttcttttgt
     7381 tcatgacctg acctgaagca gcagatttga tagactggca aagatgaata ggaaaaactt
     7441 tgactcttgc ccagtgcctg cctgcctctc tctctctcct ctctctctct ctctttttaa
     7501 accaccatag actctcactt attttggagg agtgtatgtg catgtgtaca catatgtaca
     7561 tatataaaaa cagttgatgt tttggtgtat ggggtaaaag ccccaggagc atacagaact
     7621 tgagctgaac ctggtcagta gaaagggaaa ccccaaccct ttcttgattt ttggagacac
     7681 ccaggactaa tggatactgt agtagacatg tagtactata gtaccgtaat attcatctct
     7741 cctcatttta gttgcctttc agattctaaa tgtttgggat cattcctatc atcttagtct
     7801 cttcaggatc tttagtattt tcctggttct gaggaggctt gagacagggt tatggatttc
     7861 cttgttctgt gtctttatcc ctactgatag ttcctctttc tcattttgca aactacccag
     7921 agctccaagg acagcctgaa gatctcagtg cctcaggctc tgaggtactc tgctcacagg
     7981 ccccagacag atgctgttgg gtaaactcaa agcttcctat gagaagcttc tgagatgtag
     8041 caggtcacag aagtgttcac ctgtttcatc tgaggatttc agttctgaag tgatctaggc
     8101 atgtttggta aaaatgtttt tgtgagagtt ctttttttaa gaaagcatta tgcaaaaagt
     8161 cacttttaag ggcatttaaa aatttttaag taaagccatt ctttggcttt tttggactat
     8221 ttgtggatac tttgtttaca ttttgaatat taaaatgtgc atcatcttgt cggccatgca
     8281 gtcctgcagg catcagtgcc ctggctgtcc tgtgagtcct catgcagagc agggccatca
     8341 tggtcagccc ttgtgagtaa gtgggtatac gctgaagctg acttccgtct gtgtcctgcc
     8401 ctcccatcca gcactctcta tggaaatagt tgtcatttcc tcagtgatgg tgtgtcttat
     8461 agtgatggtt gtaatggtga atggctagtg tcattttgtt taattttgtt ttgctttttg
     8521 atggtggata agatgaaatt ttagctgttt cagcatcaat ccaacttaaa agcatcttgg
     8581 actttgtttt cccttccaaa ctaaaagatc aaggcatgta ttagacacaa gcatatttgc
     8641 tgcctcaact cctctgacac ccatattagg aaaaggcctg ccaccaaagc aggaagctgc
     8701 acactcctaa cccttctccc agggtgtggg gtcccttgac gtcatgaaga gctattgcaa
     8761 ggtgcttctg tacttttcca gcctggaaat ggagggtgac tggccctggc tcctgctatg
     8821 agaagataac cagcctcatt ttctcagtgc cccagaggtc taggatagga tttctaaact
     8881 ggaatcatcc ttaatcacct tgaagatccc ttaagaggca tttgactggt gctgccgtct
     8941 gtgtcctcaa agcaatgctg gtggcagcgt cctgtgtaca catgcagagc taatacccaa
     9001 actaaaaact gggtaactgg ccctgaagtg cttcccaatc agtaagccca cagggaaatg
     9061 tttgattttt atgttctgtt ggattttggt ttgcttggca tatctaaagg tgcctttact
     9121 tttctttttt tttttttttc tttctgcttt gttttgtagg acttgttcta acatggaaaa
     9181 caagtccaga agactctcct ctgactgtta cctttgcccc aagccacccc aaacttttat
     9241 gctcatgttt tattaaagca ggtgctccct ggaatctctg ggacattttt gaggcatttg
     9301 aagcagaata tagagtggtc tcatctcctt ccttaatctt cctggtggtt gggatgttcc
     9361 acttgtatca tagatttttt tattacagat atgctccact gtttttaaat gtgaacttgt
     9421 gcgcaaatgt gcagattcaa tgttcttgtt acagattgaa taaattttta ttttgaagat
     9481 gaaa
//
