LOCUS       NM_018984              13034 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens slingshot protein phosphatase 1 (SSH1), transcript
            variant 1, mRNA.
ACCESSION   NM_018984
VERSION     NM_018984.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Lai,S.W., Cheng,Y.C., Huang,W.C., Yadav,V.K., Fong,I.H., Yeh,C.T.,
            Yang,C.K., Lee,W.H. and Chen,M.Y.
  TITLE     Dysregulated expression of slingshot protein phosphatase 1 (SSH1)
            disrupts circadian rhythm and WNT signaling associated to
            hepatocellular carcinoma pathogenesis
  JOURNAL   Aging (Albany NY) 15 (20), 11033-11051 (2023)
   PUBMED   37837551
  REMARK    GeneRIF: Dysregulated expression of slingshot protein phosphatase 1
            (SSH1) disrupts circadian rhythm and WNT signaling associated to
            hepatocellular carcinoma pathogenesis.
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   Cazzaro,S., Woo,J.A., Wang,X., Liu,T., Rego,S., Kee,T.R., Koh,Y.,
            Vazquez-Rosa,E., Pieper,A.A. and Kang,D.E.
  TITLE     Slingshot homolog-1-mediated Nrf2 sequestration tips the balance
            from neuroprotection to neurodegeneration in Alzheimer's disease
  JOURNAL   Proc Natl Acad Sci U S A 120 (30), e2217128120 (2023)
   PUBMED   37463212
  REMARK    GeneRIF: Slingshot homolog-1-mediated Nrf2 sequestration tips the
            balance from neuroprotection to neurodegeneration in Alzheimer's
            disease.
REFERENCE   3  (bases 1 to 13034)
  AUTHORS   Chen,F., Aye,L., Yu,L., Liu,L., Liu,Y., Lin,Y., Gao,D., Gao,Q. and
            Zhang,S.
  TITLE     SSH1 promotes progression of intrahepatic cholangiocarcinoma via
            p38 MAPK-CXCL8 axis
  JOURNAL   Carcinogenesis 44 (3), 232-241 (2023)
   PUBMED   36857607
  REMARK    GeneRIF: SSH1 promotes progression of intrahepatic
            cholangiocarcinoma via p38 MAPK-CXCL8 axis.
REFERENCE   4  (bases 1 to 13034)
  AUTHORS   Cazzaro,S., Zhao,X., Zhao,V.K., Kim,Y.K. and Woo,J.A.
  TITLE     Slingshot homolog-1 amplifies mitochondrial abnormalities by
            distinctly impairing health and clearance of mitochondria
  JOURNAL   Hum Mol Genet 32 (10), 1660-1672 (2023)
   PUBMED   36637427
  REMARK    GeneRIF: Slingshot homolog-1 amplifies mitochondrial abnormalities
            by distinctly impairing health and clearance of mitochondria.
REFERENCE   5  (bases 1 to 13034)
  AUTHORS   Song,X., Xie,D., Xia,X., Tan,F., Pei,Q., Li,Y., Zhou,Z., Zhou,Y.,
            Li,C., Wang,K. and Pei,H.
  TITLE     Role of SSH1 in colorectal cancer prognosis and tumor progression
  JOURNAL   J Gastroenterol Hepatol 35 (7), 1180-1188 (2020)
   PUBMED   32020663
  REMARK    GeneRIF: Role of SSH1 in colorectal cancer prognosis and tumor
            progression.
REFERENCE   6  (bases 1 to 13034)
  AUTHORS   Nishita,M., Wang,Y., Tomizawa,C., Suzuki,A., Niwa,R., Uemura,T. and
            Mizuno,K.
  TITLE     Phosphoinositide 3-kinase-mediated activation of cofilin
            phosphatase Slingshot and its role for insulin-induced membrane
            protrusion
  JOURNAL   J Biol Chem 279 (8), 7193-7198 (2004)
   PUBMED   14645219
  REMARK    GeneRIF: findings suggest that SSH1L is activated downstream of
            PI3K and plays a critical role in insulin-induced membrane
            protrusion by dephosphorylating and activating cofilin
REFERENCE   7  (bases 1 to 13034)
  AUTHORS   Ohta,Y., Kousaka,K., Nagata-Ohashi,K., Ohashi,K., Muramoto,A.,
            Shima,Y., Niwa,R., Uemura,T. and Mizuno,K.
  TITLE     Differential activities, subcellular distribution and tissue
            expression patterns of three members of Slingshot family
            phosphatases that dephosphorylate cofilin
  JOURNAL   Genes Cells 8 (10), 811-824 (2003)
   PUBMED   14531860
REFERENCE   8  (bases 1 to 13034)
  AUTHORS   Kaji,N., Ohashi,K., Shuin,M., Niwa,R., Uemura,T. and Mizuno,K.
  TITLE     Cell cycle-associated changes in Slingshot phosphatase activity and
            roles in cytokinesis in animal cells
  JOURNAL   J Biol Chem 278 (35), 33450-33455 (2003)
   PUBMED   12807904
  REMARK    GeneRIF: SSH1 plays a critical role in cytokinesis by
            dephosphorylating and reactivating cofilin in later stages of
            mitosis
REFERENCE   9  (bases 1 to 13034)
  AUTHORS   Endo,M., Ohashi,K., Sasaki,Y., Goshima,Y., Niwa,R., Uemura,T. and
            Mizuno,K.
  TITLE     Control of growth cone motility and morphology by LIM kinase and
            Slingshot via phosphorylation and dephosphorylation of cofilin
  JOURNAL   J Neurosci 23 (7), 2527-2537 (2003)
   PUBMED   12684437
REFERENCE   10 (bases 1 to 13034)
  AUTHORS   Niwa,R., Nagata-Ohashi,K., Takeichi,M., Mizuno,K. and Uemura,T.
  TITLE     Control of actin reorganization by Slingshot, a family of
            phosphatases that dephosphorylate ADF/cofilin
  JOURNAL   Cell 108 (2), 233-246 (2002)
   PUBMED   11832213
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP363810.1, AB072355.1,
            AC087893.15, BC020243.1 and AI621348.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_018984.3.
            
            Summary: The protein encoded by this gene belongs to the slingshot
            homolog (SSH) family of phosphatases, which regulate actin filament
            dynamics. The SSH proteins dephosphorylate and activate the actin
            binding/depolymerizing factor cofilin, which subsequently binds to
            actin filaments and stimulates their disassembly. Cofilin is
            inactivated by kinases such as LIM domain kinase-1 (LIMK1), which
            may also be dephosphorylated and inactivated by SSH proteins. The
            SSH family thus appears to play a role in actin dynamics by
            reactivating cofilin proteins. Alternatively spliced transcript
            variants encoding different isoforms have been described for this
            gene. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1, also known as
            SSH-1L). This isoform has been shown to be enzymatically active
            (PMID:11832213).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062341.1, AB072355.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000326495.10/ ENSP00000315713.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                BP363810.1         1-87
            88-2814             AB072355.1         1-2727
            2815-6181           AC087893.15        52904-56270         c
            6182-8293           BC020243.1         1-2112
            8294-8535           AI621348.1         3-244               c
            8536-13034          AC087893.15        46051-50549         c
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.11"
     gene            1..13034
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /note="slingshot protein phosphatase 1"
                     /db_xref="GeneID:54434"
                     /db_xref="HGNC:HGNC:30579"
                     /db_xref="MIM:606778"
     exon            1..156
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..3237
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein phosphatase Slingshot homolog 1; hSSH-1L; SSH-like
                     protein 1; slingshot homolog 1"
                     /codon_start=1
                     /product="protein phosphatase Slingshot homolog 1 isoform
                     1"
                     /protein_id="NP_061857.3"
                     /db_xref="CCDS:CCDS9121.1"
                     /db_xref="GeneID:54434"
                     /db_xref="HGNC:HGNC:30579"
                     /db_xref="MIM:606778"
                     /translation="MALVTLQRSPTPSAASSSASNSELEAGSEEDRKLNLSLSESFFM
                     VKGAALFLQQGSSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESAWA
                     DRVRYMVVVYSSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFS
                     VSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQ
                     SCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEI
                     RNELEKQMNCNLKELKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSG
                     VDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVH
                     CKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDAS
                     KQRHNKLWRQQTDSSLQQPVDDPAGPGDFLPETPDGTPESQLPFLDDAAQPGLGPPLP
                     CCFRRLSDPLLPSPEDETGSLVHLEDPEREALLEEAAPPAEVHRPARQPQQGSGLCEK
                     DVKKKLEFGSPKGRSGSLLQVEETEREEGLGAGRWGQLPTQLDQNLLNSENLNNNSKR
                     SCPNGMEDDAIFGILNKVKPSYKSCADCMYPTASGAPEASRERCEDPNAPAICTQPAF
                     LPHITSSPVAHLASRSRVPEKPASGPTEPPPFLPPAGSRRADTSGPGAGAALEPPASL
                     LEPSRETPKVLPKSLLLKNSHCDKNPPSTEVVIKEESSPKKDMKPAKDLRLLFSNESE
                     KPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSVPADPAPPSRDGPASRLEASIP
                     EESQDPAALHELGPLVMPSQAGSDEKSEAAPASLEGGSLKSPPPFFYRLDHTSSFSKD
                     FLKTICYTPTSSSMSSNLTRSSSSDSIHSVRGKPGLVKQRTQEIETRLRLAGLTVSSP
                     LKRSHSLAKLGSLTFSTEDLSSEADPSTVADSQDTTLSESSFLHEPQGTPRDPAATSK
                     PSGKPAPENLKSPSWMSKS"
     exon            157..197
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            198..301
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            302..366
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            367..488
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            489..557
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            558..623
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            624..818
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            819..912
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1041
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1088
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1235
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1436
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1980
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..13034
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8508..8513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /note="hexamer: AATAAA"
     polyA_site      8528
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /note="major polyA site"
     regulatory      12610..12615
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /note="hexamer: AATAAA"
     polyA_site      12636
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
     regulatory      13012..13017
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
                     /note="hexamer: AATAAA"
     polyA_site      13034
                     /gene="SSH1"
                     /gene_synonym="SSH1L"
ORIGIN      
        1 aggaggcccc gggcccggcc cgggcggcgg tggcggtggc ggctctagct cgagacgtct
       61 gtggcgccct cgcaccgcgc cgcagccatg gccctggtga ccctgcagcg ctcgcccacg
      121 cccagcgccg cctcctcctc ggccagcaac agcgagttgg aggctggcag cgaagaagat
      181 cgaaaattaa acctcagctt aagtgagagc tttttcatgg tgaaaggcgc agccctcttc
      241 ttacaacagg gaagcagccc tcaaggccag cggagtcttc agcaccccca caagcatgca
      301 ggtgatctgc ctcaacatct tcaggtgatg atcaaccttc tgcgttgcga agacagaatc
      361 aagctggcag tgcgcctgga gagcgcctgg gcggaccggg tccggtacat ggtggtggtg
      421 tacagcagcg ggcgccagga caccgaggag aatatcttgc tgggagtgga cttttccagt
      481 aaggaaagta aaagctgcac cattgggatg gttctccgac tgtggagcga cacgaaaatc
      541 caccttgatg gagatggtgg gttcagcgtg agcacagcag gaaggatgca catatttaag
      601 cctgtgtctg tccaggccat gtggtctgcc ctgcaggtgc ttcacaaggc ctgcgaagtg
      661 gcccggaggc acaactactt ccccgggggt gtagctctca tctgggctac ctactatgag
      721 agctgcatca gctccgagca gagctgcatc aacgagtgga acgccatgca ggacctggag
      781 tctacgcggc ccgactcccc cgcgctattt gtggacaagc ccactgaagg ggaaaggacc
      841 gagcgcctca tcaaagccaa gctccgaagc atcatgatga gccaggatct agaaaatgtg
      901 acttccaaag agattcgtaa tgaattagag aaacagatga attgtaactt gaaggaactc
      961 aaggaattta tagacaatga gatgctactt atcttgggac agatggacaa gccctccctt
     1021 atcttcgatc atctttatct cggctctgaa tggaatgcat ccaatctgga ggaactgcag
     1081 ggctcagggg ttgattacat tttaaatgtt accagagaaa tcgataattt ttttcctggc
     1141 ttatttgcat atcataacat ccgagtctac gatgaagaga ccacagacct cctcgcccac
     1201 tggaatgaag cgtatcattt tataaacaaa gcgaagagga accattccaa gtgcctggtg
     1261 cattgcaaaa tgggcgtgag tcgctcggcc tccacagtca tagcctatgc aatgaaggaa
     1321 ttcggctggc ctctggaaaa agcatataac tatgtaaagc agaagcgcag catcacgcgc
     1381 cccaacgcgg gctttatgag gcagctgtct gagtatgaag gcatcttgga tgcaagcaaa
     1441 cagcggcaca acaagctgtg gcgtcagcag acagacagca gcctccagca gcctgtggat
     1501 gaccctgcag gacctggcga cttcttgcca gagaccccag atggcacccc ggaaagccag
     1561 ctgcccttct tggatgatgc cgcccagccc ggcttagggc cccccctccc ctgctgtttc
     1621 cggcgactct cagaccccct tctgccttcc cctgaggatg aaactggcag cttggtccac
     1681 ctggaggatc cggagaggga ggctctgttg gaggaagctg ctccacctgc agaggtgcac
     1741 aggccggcca gacagcccca gcaaggttcc ggactctgtg agaaggatgt gaagaagaaa
     1801 ctagagtttg ggagtcccaa aggtcggagc ggctccttgc tgcaggtgga ggagacggaa
     1861 agggaggagg gcctgggagc agggaggtgg gggcagcttc caacccagct cgatcaaaac
     1921 ctgctcaact cggagaacct aaacaacaac agcaagagga gctgtcccaa cggcatggag
     1981 gatgatgcta tatttgggat ccttaacaaa gtgaagcctt cctataaatc ctgtgccgac
     2041 tgcatgtacc ctacagccag cggggctcct gaggcctcca gggagcgatg tgaggacccc
     2101 aatgctcccg ccatctgcac ccagccagcc ttcctacccc acatcacgtc ctcccctgtg
     2161 gcccacttgg ccagcaggtc ccgtgttccg gagaagccag cctctggccc aaccgaacct
     2221 cccccgttcc taccaccagc aggctccagg agggcagaca ccagtggccc tggggctgga
     2281 gctgccctag agccaccagc cagccttttg gaaccttcca gagagacccc aaaagtcctg
     2341 ccaaagtccc tccttttgaa gaattctcac tgtgataaga accctcccag cacagaagtg
     2401 gtaataaagg aagaatcgtc acccaagaaa gatatgaagc cagccaagga cctgaggctt
     2461 ctgttcagta atgaatctga gaagccgaca accaacagct acctgatgca gcaccaggag
     2521 tccatcattc agctgcagaa ggcaggcttg gtccgcaagc acaccaaaga gctagagcgg
     2581 ctgaagagcg tgcctgcaga cccagcacct ccctccaggg atggccctgc cagcaggctg
     2641 gaggccagca tccccgagga gagccaggat ccagccgcgc tccacgagct gggccccctg
     2701 gttatgccca gccaggccgg gagtgatgag aagtcagagg ccgcccccgc ttcattggaa
     2761 ggaggctcac tgaagagccc ccctcctttc ttctaccgcc tggaccacac cagtagtttc
     2821 tcaaaagact ttctgaagac catctgctac acccccacct cctcttccat gagctccaac
     2881 ctgacccgga gctccagcag cgatagcatc cacagtgtcc gtgggaagcc cgggctggtg
     2941 aagcagcgga cacaggagat tgagacccgg ctccggctgg cgggcctcac cgtctcttcc
     3001 ccactgaagc gctcacactc tcttgccaag ctggggagtc tcaccttctc aacggaagac
     3061 ctgtccagtg aggctgaccc gtccaccgtc gctgactccc aggacaccac tttgagtgaa
     3121 tcttccttct tgcatgagcc ccagggaacc ccgagggacc cagctgcaac ctccaaacca
     3181 tcagggaaac ccgccccaga aaacttaaag agcccttcgt ggatgagcaa aagctgaccc
     3241 gccttttgct gcgtcaggct gggcggatat tttcatatga tccctccctc actttcactg
     3301 tggatttgga tcgacccctt acatcttgat ttcccttaaa cgagtgaagt catcctaact
     3361 tttcccctac tcttgccata gagaggagga gaagaaacta accaacatgc agcacaagaa
     3421 ggggagctgt cagtcgtgtg gcctgggaga accaggagcc gccggccagg agacacaaaa
     3481 ctccgctccc accgtgtctt caagaagaac catgttttag gaagaacgtg cacacatagg
     3541 cgcacacatc cagactgttc ccttcgcatc ctgcagaaga gtcctggtgg tggcagcctc
     3601 accgcggcac acgttctagc tcattcttgg cctcgcagaa aactctcgga tggcaacatt
     3661 aagtcctacc tctgttctcg ctgcgctttt tatttttaaa acacacgaat gaccaaggct
     3721 tgttccaggg aataatgctc tgtctgaaag cgtttttcca aaaaaagaat ttttcgtttt
     3781 gtgttttggt tgggacccca gacgtgtcaa gacttttaac ccgacagccc caagcactgc
     3841 tgagtaaagt catcagatga gtagtcccac gctgttgttg agctgactct gtgcccgaat
     3901 ggtggtgcag gcgccctgtc ctggttgtgt tgttacgtgt ttgaccagca caggggtccg
     3961 gtggggaaaa tgtatgggtt gttctcagtt gttgctaatg ctgaagttta aatctcaagg
     4021 ggaagggccc acctgcattg ttgagtgtct gctgctgaaa cacatgattg tgtttaggtt
     4081 tgaaattgct caagtgtctg gctcaggtgg tggttctgag acacatcgtc ctgctgagag
     4141 cccagatgct taggtccact agggcccatc tagggaaggg aaaggagatt tcagcggctt
     4201 ccccgaaagg aacgggactg tcgggatgct tcccggatgt ctacagttgc cccttcctgc
     4261 agtgagatta ctgcttcctg tttccctcca gctcttccca gcagcagtga gggagtataa
     4321 gagggatctg tagtcgctgc ctggctctgt gggcggcccc tttaagactc aggtgagctc
     4381 agccagtccc gctgcgccag gcttgaatca aaggtgtcag caagctagat gtcaaaacat
     4441 ttcgagagag aggcagcttg cagaaaagca gaaaggttga attcaggggt cagcaaacta
     4501 tggcatgtgg ccccgtgagc taggaatggt ttttcctctt taagacaggg cctggctctg
     4561 tcacctaggc tggagtacag tagtgcaatc atgactcact gcagcatcaa cctccctggc
     4621 tcaagcgatg gcttttacct ttttaaggag ttgtaaaaaa aagaaataat atgtgacaga
     4681 attcatgtgg cccacaaagc ctgaggtact tactgtcagg ccctttgtag aaaaagttgc
     4741 tgacccctgg tttgatttgc cacacagggc tgctcatggc cctgtaggaa gaaactgggt
     4801 aaatgtgaag ccctctgcct ctctgacacc taaggccaga cccttccctc ctcccgacca
     4861 caagctttgc cagccccgca ctagcctcac ctgtgcagga tggagtaggt tgcgtacatg
     4921 ggggaaggca ggtgaacaca gttggctgaa gctcttccac aattccatct tgccctcagc
     4981 tgggtccgcc agattaactc agtgaaacca gaaagccttc aaggaccagc tgaattctga
     5041 aagtgagtga gtgagccatc atcttaacat tggccagaac tgtgttcccc aaagctattc
     5101 tagaaagcac cccaggaggg atctgcagga acaaggctag ttcatatttt acctagtgag
     5161 cacagttttt gcaaaaatcc tccctccagg actttgtctc cttggagtga tttttaaaaa
     5221 tacatacatt actttatagg gagctgtttt cccactagtg tcaattaaaa tcaccttaaa
     5281 aggtgattat ccacttattc ctaaacccct gtgggttgtt ccccctttcc ctcagccaac
     5341 aaaagcatag cctcaaaaaa tatcaagttc ggtatgtttt gccaaatcaa atttcatgtg
     5401 gtagatcaat ttttgtgtca aaataatctt ttaaatttag tgatgacagg cttttgttgg
     5461 ttttttaacc acgtctatgt atgagaatga tatatttttg aaaactttaa ttttgaaagc
     5521 cataattttt cttatctaaa gagttggggg gtggggtgtg gaatctggag agtacaagtt
     5581 ggtctttggc ttctggcaaa cttacccatt catttttgga agcacagcta gcatatcaac
     5641 atccagacga gagcgctggt cccgtccaca gagcagagtg aagcattctg gacttgatgc
     5701 ttaatagcct ggcctggaga aaagggtaag gtttattttt ggaaacccag atcagttgca
     5761 tgtaaacaga tggcacatgg ctatttaaaa tgctgtatga tggggccggg cgcggtggct
     5821 cacgcctgta atcctagcac tttgggaggc cgaggcaggc ggatcatctg aggtcaggag
     5881 ttctagacca gcctggccaa catggtgaaa ccccgtctct actaaaaata caaaaactta
     5941 gctgggtgtg gtagtgcgtg cctgtaatcc tagctactta ggaggctgag gcaggagaat
     6001 cgcttgaacc tgggtggcgg aggttgcaat gagccgagat cgcgccactg cactccagcc
     6061 tgggtgcaat gaaactggga gtgaaactcc gtctcaaaaa aataaaatgc tgtatgaaca
     6121 agatgagcat tctgtcaggt gtcgggacac ctgggcaaag acgaattcat gctgtctgtg
     6181 aaaaggaagt ttgcactgta acatatgcca tagcttggcc cttgctttgt atgcaacctt
     6241 agctgatggg gaaaattcca aacataagtg gcacaggaaa gaggcaggca gaggaggcag
     6301 gggtctgtgc tgtgcaagtc actggttttt tatgactatc attttcattg aatgcatttg
     6361 ttgaattggg acaaaaggaa cattttctaa atcagcttga tactctttat aaaaaacagc
     6421 tgaatcttat cttctgcctc acattaattc atgtggcatg ccaaaagtta gactgaactt
     6481 ttctacatct tcaccttgca ttgatacagg aaagtcttct ctttaagtct tgctaagtat
     6541 aagggggata gtggtcacgg gaagaggaga accggggctg ctctcggatg gtaattgaca
     6601 atgccgattt ccatttcttt gtcttacggc tacttccgag ccttctagca aggtccctgg
     6661 ttgcatgtat ttcctaagag cagagtttat ttctctttac attccctgct tccttgctct
     6721 ccatgcaggg accccagcca acctctgggc tgttgaatag ctctctgctg ggtggcaagc
     6781 ccttctcctt ggcaccctgt tgcttaagca tacagattgc tcaccaggtg cacacagaca
     6841 gctgtactgt ccaagcccat tctgctccac cctgtcctct tcagctaaag agcattccag
     6901 taatctaaag cccttttccc ctggagaagt ccagagtttg cagcagtgtg gagctgagct
     6961 tgaacagaac agaccctcag cacggccctg gactaatgca ctcttatctg agggcagtcc
     7021 attctagaag gttgatgaat ttccctacca ctctcctggg gaccttttct ctctagtggg
     7081 aacaaatgct acataattta gtcaagatcc tattaagtca tctcacctga ggcatctgga
     7141 gagggagttg ccttccattg gtgggaaatt gttggtgcca gaatacattt tgcccaaaac
     7201 tcttctcatt ggctggccac ctagcagggc tcctctaaac acgcaactca gcgaggggac
     7261 ccccttcacc tctggcaaga gagctgggta gatcagaaac ttggtgacac ctggctagca
     7321 cagagcaggc tcacttgtct tggtcccact acccagattc ctgcagacat tgcaaaccaa
     7381 atgaaggttg ttgaatgacc cctgtcccca gccacttgtt ttgttatcat ctgctctgca
     7441 gtggaatgcc tgtgtgtttg agttcactct gcatctgtat atttgagtat agaaaccgag
     7501 tcaagtgatc atgtgcatcc agacacactg tgtcacctga gccacagagc aaatcacctt
     7561 aacgatctgg aatgaaactg tgaccagtgc cgccctgggt ggttctggag agactgccgt
     7621 cttcttgttt ggccataggt gctggggccc cggcttcagt cactgtctca gacaggagtc
     7681 ccgataagca gatcaccagt cctccactgt ccttcctgtc ggccttgctg catgagaaga
     7741 tagctgcttc ctccctcttt tcctacactg taattattgt tttacaattg agtgccttaa
     7801 taatagttta caaatactat gtatttatgc aaaactgtta aagtctcatc tgttatgatt
     7861 ggatacttgg tcttgtcagt agtggtcagc attgggttgt gagcttgtcc tactccatac
     7921 gtgtttatcc tgctatgcag ttttacattg tgtgttcaca tctattccaa ggagccttgc
     7981 tagaaacaac actggcggtt cctgcaggcc aggcaggcat tggcccatgc tgtgtcccat
     8041 aggagccaat gaaagaacgt agcttggtct gctagccagc cgtggggtgg cgcaggccag
     8101 gcagcctctg caccagagtc cagcacctgc ccattcccca gtcacacaat catactcttc
     8161 tttcatagag attttattac cacctagacc accctagttt tcctctctgt tagtgtcctg
     8221 agctcttttg caacaaaatg taggtacaga ccaatccctg tcccttcccc aatcaggagc
     8281 tccacaccat gagttgtttg gttttccaga agctgccagt gggttcccgt gaattgcgtt
     8341 aagatatcga tgattttttt tattgttttt cttcttgttt ttttaaataa tatatttaaa
     8401 ggcagtatct tttgtactgt gaatttgcag tagaagatgc agaatgcact ttttttttac
     8461 ttctgttggt gtgtattgta tatagtgtgt gtgcttcttg tgatgaaaat aaactttttc
     8521 tttataaatt cctgatgact gagtttatat aaaaggaaac tgcttcctca gcccttgcca
     8581 aaacagtctg atacatcgtc atcatcatcg tcattgacat ggccagtctt tattgagcat
     8641 ctattacatg ctcagttctt tgctagaaaa cctggacaag ttacaccaaa gaaaggacca
     8701 caggaagaaa tgatagttcc tcctggaact agtctgtttt ctttctaatt ttgtcagtaa
     8761 gcaagaacag gcccacctgt gggtggtcct ttgtgtacca aaaaaaaaaa gttacccacc
     8821 aggaaaggat ttggactttg ggtgggttct cttcccgtac aagtaggaaa tgtaaagcca
     8881 gaggtgtgca ctaaagaaca tctaacctgc tcccaccagc cctggtggga gagggaggac
     8941 cctggaggct aagtactgct ggtttccaag gtgcatgctc atgtcccagt ccacttcacc
     9001 ccaggcagct tttggtggct cttctccctt ggacactcag gaggcagagc tgcttccatt
     9061 tttttttttt taattttttt tttttttttt ttgagactgg gtctggctct cttgctcagg
     9121 ctggagttca gtggtgccat catagctcac tgcaacctcg acttcctagg ctaaagtgat
     9181 cctcctgcct caagcaatcc tcccacctca gcctccccag tagctgggac tacaggcgtg
     9241 caccaccttg ccccgctaat ttttacaatt tattatagac atgaagtctc gctttgctgc
     9301 ccaggctggt ctttaattcc tgtcttcaag tgatcctccc accttggcct cccaaagtgc
     9361 tgggattaca ggtgtgagcc acctcaactt ccatcttaaa attctgcctc cttagagttc
     9421 tttgctttct gctgtgtagt cagggaggag tcacattcac tcagtctcct ctgtctggat
     9481 gcgatacatc atttctactt gctttccagt gggcagggcc cagtcacagg gtcccaatgc
     9541 aacagccagg gagactggga aatgtatggg gcacatgtat ttggtgagca ctaactgtca
     9601 caagtacctg ggcgtgaccc ctaaattcca gtgcctgcag agccaggaag tattttgttc
     9661 agttcatctg gacttgctct gtctctcaac tggactcaaa agactgatga ataaaccaga
     9721 atgcaagttt attactttac caaaagaatt ataatcatca agcagaccag attgctcaca
     9781 tgctgattag cacatttagt cctgctaggt aagtctttct ttttttgaga cagcctggag
     9841 tgcagtggca gtcatggctc actgaagtgt tgatctcctg ggctccagca atcctgcctc
     9901 agcctcccaa gtagctggga ccagagccac acactgctgc acccagctaa atttatttat
     9961 ttatttattt aaaagatagg gtctcactat gttgcccaga ctggtcttga actcctggtt
    10021 tctgatgatc ctcctgcctc agcctcccaa agtgttgaga ttataggcat gagccactgt
    10081 gcccagcccc ttctaggtaa atctctatgg ggtgccaaat tttggttaag aattgcttat
    10141 tgaatattaa aagtttagtg ggatgcttct tttttttttt tcttttgaga tggagtttag
    10201 ctcttgttgc ccagactgga gtgcaatggt gtgatctcag ctcaccgcaa cctctgcctc
    10261 ctgggttcaa gtgattttcc tgcctcagcc tcccgagtag ctgggaatac aggcatgtgc
    10321 caccacgcct ggctaatttt gtatttttaa tcgaaatggg gtttctccat gttggtcagg
    10381 ctgttctcaa actcccaacc tcaggtaatc cacccgcctt ggcctcccaa agtgctggga
    10441 ttataggcat gagccaccgc gccaagccac aggatgcttc taatagtgtt atcaagctat
    10501 aatgcagccc tctctgtaaa atgttttaaa aacaaatcac tgagggtcat ggttcaagac
    10561 actgttctct ggcaaggttg aagatagcta acaaattctc tttgggttcc agccatgaat
    10621 ggggtgggat gggccccagc aatctgtatg tttctgagat tccctgggaa ttctgatggc
    10681 caagtttaga aaccatcaaa ataagaatat gatgcaactg agtaggctca cgctttgcta
    10741 attacattgc acacatgctt tttggagaca acacccacac taccctaaca atgtgtgttc
    10801 ataaaactca tccgatactt atttgttcaa ggccctagga caatattcct gtaatgggct
    10861 gcttttgccc tgatttcctc ctcagagtaa ctgtctcgcg gttgggcacg atcccagatc
    10921 acatctacag tggaggtggt tttgctgggg aggatccgca tgcttgtttg tgtttatgcc
    10981 tggggaaccc cctcttggca agatgttcgg gagaggctcc aaaggcagca cagaggacgg
    11041 cttggaactt agggggattc actttatggt aaagaagccc tttctaaagg agagctccac
    11101 ccaggaaaca ccacccaccc acgcatcctt gcctcctaaa accaggttat gtttatcaca
    11161 taccttctat gtgctttaca taaagaatcc cctggaattc tccaaactgg gcattgtggt
    11221 ctgtgatgca tatctcaaaa gaggtgagag ggttgggcac ggtggctcac gcctgtaatc
    11281 ccagcacttt gggaagccaa ggcaggtgga tcacttgagg tcagaagctc aagaccagcc
    11341 tggctcacat ggtgaaacgc cgtctcaact aaaaatacaa aaattagccg ggcatgatgg
    11401 cgggcgcctg taatcccagc tactcgggag gctgaggcag gagaatcgct tgaacccagg
    11461 aggtggaggt tgctgtgagc caagattgca ccactgcact ccagcctggg tgacagagtg
    11521 agactctgtc tcaaaaaaca aaaaaaagaa aaaaaaaggt agaggagagt cctttttcta
    11581 actttcttga actctttgga ctaccttggg atccttctat taactgcctt gttggcatta
    11641 ttctcagttt atgaaaaaac aggcccacag agtggaagta atttgcctac aggtacatct
    11701 aaaaattgat gaagccagat aggaactgct ccctcatcat ctgaaaccct tcccagagct
    11761 gtctctccca ccccagaagt agaaagaagc cagacaaaga tgcatcttga tctcccttgt
    11821 ggtttcagca aggaagcaga tgagttggag atgaagcccc cagcccctgg gaaattggac
    11881 ccacttcttg ctccaaaggc tattggggtg acacaaggtg tgtttctttc ccgtagatca
    11941 tattcaccat attttgcctt taaatggaaa ttgtgcatcc tgaagtacct tgttaagagt
    12001 ccattttcaa cctgaaattc cttttttttg taaaccactt cagcaaatac agtgtggcat
    12061 ttacattagc aacctcccag cctaggagag gatgctccag catccgactc taaaagacaa
    12121 atttgggcac ggtggcttga cgcctgttat cccaacacct tgagaggccg aggcaggcag
    12181 attgcttgag cccaggagtt caagaccagc ccaggtaaca tggtgaaccc ccatctctac
    12241 caataataca aaaattagcc aggcatggtg gtgtgccagt ggtcccagct acttgagtgg
    12301 ctgaggtgaa ggatctcctg agcctgggag gcagaggttg cagtgagccg agatcgaacc
    12361 actgcactcc agcttatgca acagagcaag accgtgtctc aaaaacaaat aaaaataaaa
    12421 gattttaaaa agacaaatta tcccacaaat ggtacattgt ggtggtggtg gtggtggtgg
    12481 tggtagacct ccctcttgct cttgaaattg gatcagaaca aactgacaat gctacctact
    12541 tcatggggtc attggtgaat tcactgataa tgcatgcaaa tccccttctg cctggcacac
    12601 tacctaataa ataaatggtg gttgttattg ttggaattgg aggatatatt cctagttgca
    12661 agggtttcac atgtattacc aagttaatcc tcaccgcaga tatgattctg ttttacagag
    12721 gaagcagtgg atgctcagag aggttaggta acgtgggtca gggacacaca gccaggaagg
    12781 ggcagaggct gggagtgcct gactccaaat tcatgcaatt tctaccccac catcttctcc
    12841 ccaaggaaaa tagtccctaa tgcatgtgtg caatgcttag agggcagtag atgctcaaca
    12901 gatgctgaga gagtgaggga gtaaatgaga tatgccatga aaaggagccc ctgtggactt
    12961 atgcgtgcgt ttgtttaact tgtgggcaag tacttataga caggtgcaaa caataaatct
    13021 ccttttgcaa ctga
//
