LOCUS       NM_006924               5343 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens serine and arginine rich splicing factor 1 (SRSF1),
            transcript variant 1, mRNA.
ACCESSION   NM_006924
VERSION     NM_006924.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Paul,T., Zhang,P., Zhang,Z., Fargason,T., De Silva,N.I.U.,
            Powell,E., Ekpenyong,E., Jamal,S., Yu,Y., Prevelige,P., Lu,R. and
            Zhang,J.
  TITLE     The U1-70K and SRSF1 interaction is modulated by phosphorylation
            during the early stages of spliceosome assembly
  JOURNAL   Protein Sci 33 (8), e5117 (2024)
   PUBMED   39023093
  REMARK    GeneRIF: The U1-70K and SRSF1 interaction is modulated by
            phosphorylation during the early stages of spliceosome assembly.
REFERENCE   3  (bases 1 to 5343)
  AUTHORS   Segovia,D., Adams,D.W., Hoffman,N., Safaric Tepes,P., Wee,T.L.,
            Cifani,P., Joshua-Tor,L. and Krainer,A.R.
  TITLE     SRSF1 interactome determined by proximity labeling reveals direct
            interaction with spliceosomal RNA helicase DDX23
  JOURNAL   Proc Natl Acad Sci U S A 121 (21), e2322974121 (2024)
   PUBMED   38743621
  REMARK    GeneRIF: SRSF1 interactome determined by proximity labeling reveals
            direct interaction with spliceosomal RNA helicase DDX23.
            Erratum:[Proc Natl Acad Sci U S A. 2024 Jul 23;121(30):e2412536121.
            doi: 10.1073/pnas.2412536121.. PMID: 39008678]
REFERENCE   4  (bases 1 to 5343)
  AUTHORS   Li,S., Huang,X., Zheng,S., Zhang,W., Liu,F. and Cao,Q.
  TITLE     High expression of SRSF1 facilitates osteosarcoma progression and
            unveils its potential mechanisms
  JOURNAL   BMC Cancer 24 (1), 580 (2024)
   PUBMED   38735973
  REMARK    GeneRIF: High expression of SRSF1 facilitates osteosarcoma
            progression and unveils its potential mechanisms.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5343)
  AUTHORS   Sun,S., Zhang,Z., Sinha,R., Karni,R. and Krainer,A.R.
  TITLE     SF2/ASF autoregulation involves multiple layers of
            post-transcriptional and translational control
  JOURNAL   Nat Struct Mol Biol 17 (3), 306-312 (2010)
   PUBMED   20139984
  REMARK    GeneRIF: Multiple post-transcriptional and translational mechanisms
            are involved in fine-tuning the expression of SF2/ASF.
REFERENCE   6  (bases 1 to 5343)
  AUTHORS   Zuo,P. and Manley,J.L.
  TITLE     The human splicing factor ASF/SF2 can specifically recognize
            pre-mRNA 5' splice sites
  JOURNAL   Proc Natl Acad Sci U S A 91 (8), 3363-3367 (1994)
   PUBMED   7512732
REFERENCE   7  (bases 1 to 5343)
  AUTHORS   Zahler,A.M., Lane,W.S., Stolk,J.A. and Roth,M.B.
  TITLE     SR proteins: a conserved family of pre-mRNA splicing factors
  JOURNAL   Genes Dev 6 (5), 837-847 (1992)
   PUBMED   1577277
REFERENCE   8  (bases 1 to 5343)
  AUTHORS   Mayeda,A., Zahler,A.M., Krainer,A.R. and Roth,M.B.
  TITLE     Two members of a conserved family of nuclear phosphoproteins are
            involved in pre-mRNA splicing
  JOURNAL   Proc Natl Acad Sci U S A 89 (4), 1301-1304 (1992)
   PUBMED   1741384
REFERENCE   9  (bases 1 to 5343)
  AUTHORS   Ge,H., Zuo,P. and Manley,J.L.
  TITLE     Primary structure of the human splicing factor ASF reveals
            similarities with Drosophila regulators
  JOURNAL   Cell 66 (2), 373-382 (1991)
   PUBMED   1855257
REFERENCE   10 (bases 1 to 5343)
  AUTHORS   Krainer,A.R., Mayeda,A., Kozak,D. and Binns,G.
  TITLE     Functional expression of cloned human splicing factor SF2: homology
            to RNA-binding proteins, U1 70K, and Drosophila splicing regulators
  JOURNAL   Cell 66 (2), 383-394 (1991)
   PUBMED   1830244
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA939911.1, BC010264.1,
            AC015813.12 and CB044653.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006924.4.
            
            Summary: This gene encodes a member of the arginine/serine-rich
            splicing factor protein family. The encoded protein can either
            activate or repress splicing, depending on its phosphorylation
            state and its interaction partners. Multiple transcript variants
            have been found for this gene. There is a pseudogene of this gene
            on chromosome 13. [provided by RefSeq, Jun 2014].
            
            Transcript Variant: This variant (1) encodes the longer isoform (1,
            also known as ASF-1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.891509.1,
                                           SRR3476690.763386.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142670, SAMEA2144333
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000258962.5/ ENSP00000258962.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-74                DA939911.1         101-174
            75-2767             BC010264.1         1-2693
            2768-5219           AC015813.12        69067-71518         c
            5220-5343           CB044653.1         27-150              c
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q22"
     gene            1..5343
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /note="serine and arginine rich splicing factor 1"
                     /db_xref="GeneID:6426"
                     /db_xref="HGNC:HGNC:10780"
                     /db_xref="MIM:600812"
     exon            1..303
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..856
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /note="isoform 1 is encoded by transcript variant 1; SR
                     splicing factor 1; pre-mRNA-splicing factor SF2, P33
                     subunit; splicing factor 2; alternative-splicing factor 1;
                     splicing factor, arginine/serine-rich, 30-KD, A;
                     serine/arginine-rich splicing factor 1"
                     /codon_start=1
                     /product="serine/arginine-rich splicing factor 1 isoform
                     1"
                     /protein_id="NP_008855.1"
                     /db_xref="CCDS:CCDS11600.1"
                     /db_xref="GeneID:6426"
                     /db_xref="HGNC:HGNC:10780"
                     /db_xref="MIM:600812"
                     /translation="MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRD
                     IDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGG
                     GGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVV
                     EFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSR
                     SNSRSRSYSPRRSRGSPRYSPRHSRSRSRT"
     exon            304..488
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /inference="alignment:Splign:2.1.0"
     exon            489..661
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /inference="alignment:Splign:2.1.0"
     exon            662..5343
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2747..2752
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /note="hexamer: AATAAA"
     polyA_site      2769
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /note="major polyA site"
     regulatory      2881..2886
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /note="hexamer: ATTAAA"
     polyA_site      2902
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
     regulatory      5159..5164
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /note="hexamer: ATTAAA"
     polyA_site      5187
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
     regulatory      5320..5325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
                     /note="hexamer: ATTAAA"
     polyA_site      5343
                     /gene="SRSF1"
                     /gene_synonym="ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a"
ORIGIN      
        1 gccattttgg aggggccgcg ggagacgtgg tgccgctgcg ggctcgctct gccgtgcgct
       61 aggcttggtg ggaaggcctg ttctcgagtc cgcgcttttc gtcaccgcca tgtcgggagg
      121 tggtgtgatt cgtggccccg cagggaacaa cgattgccgc atctacgtgg gtaacttacc
      181 tccagacatc cgaaccaagg acattgagga cgtgttctac aaatacggcg ctatccgcga
      241 catcgacctc aagaatcgcc gcgggggacc gcccttcgcc ttcgttgagt tcgaggaccc
      301 gcgagacgcg gaagacgcgg tgtatggtcg cgacggctat gattacgatg ggtaccgtct
      361 gcgggtggag tttcctcgaa gcggccgtgg aacaggccga ggcggcggcg ggggtggagg
      421 tggcggagct ccccgaggtc gctatggccc cccatccagg cggtctgaaa acagagtggt
      481 tgtctctgga ctgcctccaa gtggaagttg gcaggattta aaggatcaca tgcgtgaagc
      541 aggtgatgta tgttatgctg atgtttaccg agatggcact ggtgtcgtgg agtttgtacg
      601 gaaagaagat atgacctatg cagttcgaaa actggataac actaagttta gatctcatga
      661 gggagaaact gcctacatcc gggttaaagt tgatgggccc agaagtccaa gttatggaag
      721 atctcgatct cgaagccgta gtcgtagcag aagccgtagc agaagcaaca gcaggagtcg
      781 cagttactcc ccaaggagaa gcagaggatc accacgctat tctccccgtc atagcagatc
      841 tcgctctcgt acataagatg attggtgaca ctttttgtag aacccatgtt gtatacagtt
      901 ttcctttatt cagtacaatc ttttcatttt ttaattcaaa ctgttttgtt cagaatgggc
      961 taaagtgttg aattgcattc ttgtaatatc cccttgctcc taacatctac attcccttcg
     1021 tgtctttgat aaattgtatt ttaagtgatg tcatagacag gattgtttaa atttagttaa
     1081 ctccatactc ttcagactgt gatattgtgt aaatgtctat ctgccctggt ttgtgtgaac
     1141 tgggatgttg ggggtgtttg tggttatctt acctggggaa gttcttatgt ttatcttgct
     1201 tttcatgtgt ctttctgtag acatatctga agagatggat taagaatgct ttggattaag
     1261 gattgtggag cacatttcaa tcattttagg attgtcaaaa ggaggattga ggaggatcag
     1321 atcaataatg gaggcaatgg tatgactcca agtgctattg tcacagatga aattggcagt
     1381 attgacctta tactaaaagg caggggttaa aaatgattat atacattttc cttaaaacac
     1441 ttgcaaacat tttattcagt tgtctttagc tacaattgct ttgcttttta aaccttggca
     1501 attgtggcaa aattatattg cccattttgt agcaacttat tttgctccct tccccccatt
     1561 tttgttttaa tagggactaa tgtgggaaga actggctaat ttgtcacagt gcttagttac
     1621 aactgttaat gtgtgacctg ctgttggtgt acatgtgggt acagggtgtt tttaaatcca
     1681 acaagataga gtataatatc aatactgcta aatctgcatg tcctctgtgt gactgataga
     1741 gcgttgctat ttcatttttt taagacaaaa tgaaagcaaa atatagagtt ccaatgtatt
     1801 ggtgtagata atctagttgg gaatactttt aagtctcacc ttccccttta aactaatatt
     1861 cataattggt tcatatgttt aaaagacttt aatttacaaa ttaaattgca aatgggagca
     1921 ttagatttag ttttagactt aggtgggtag caatgccagt aaacttaaat tacgtaactt
     1981 cttgcaacca cgaaacctgt aatacgctgt acagtaacaa gtgttggcat tatcagttga
     2041 actgtaaata caaaatgctt cttccaatta gtctctatga tgattaagtt tctaaaattt
     2101 atctgaacac cattcagaaa cttgttttgg ggaatttgat agttattgat gtgcatctgt
     2161 taaactgatg acagacataa ctcatcattc cccagaaacc ttttttgatt acagtatcta
     2221 acattttgcc tcctcttttt tggttttgct ggttataaag gtttggattg gagagggctc
     2281 actggatccc aatccttgga gctggatcat tggattcaaa tcataatgtg gataggatag
     2341 ggaggatgaa ttaccaggat tcatggagcg ggatcagatt accaggaaca taggagtgga
     2401 ttcctgcccc aaccaaaccg cattcgtgtg gattttttta ttcaacttaa ttggctattc
     2461 caaagatttt ttttttccta tttttgacga ttggagccct taagatgcac gatggaattg
     2521 tgttttgcgt tttttggtaa aaggagcaaa gcgaggacct ggagataaac gctggagcaa
     2581 tctccttgga aggattcagc acgagtagat ggtaaacatt taaaggggaa agggggggtt
     2641 tgtttaaaat agtaaatcag taagtcactt ctaaatttaa agaaaacaaa attggagttg
     2701 aagaataagt aggtttccaa ttggctattg ccgttttctt tgaaaaaata aacatttttt
     2761 aaaaaactat gcatggttgt cctttttcct cttcatgtaa gattctaact gggtctatca
     2821 gttaatcttt aaattgttaa gtaagataag attttgactc ttgtgttaat gtgttagcaa
     2881 attaaaagtt cttaaaaggc aatctaatgg tattagccat cttttattgt taattgtaaa
     2941 agtcttcagg ggaaagcaaa aggggagaat aaggcatttg tgtatgtaac ttggtaaatg
     3001 acggtggggg atggatctag catctgaaag ataagcttct ctactttgtt ataaagtggt
     3061 taaaaaacta tagatgctgc ttattttctg gtggtcatag acaacatagg cttttgtgca
     3121 aaattggttg atggctacta atgttcactt ggagatagct tttgatattc tcaatgaaac
     3181 tcatctcaaa aaaaggtaag tattaaatgt taacatcagc acagatgtat tagaactgtt
     3241 ttttgttttt gagacagagt ctcgctctgt tctccagact ggagggcagt ggtgtgatct
     3301 aggctcactg caacctccac ccctggattt gagtgattct cgtgcctcag tctcccaagt
     3361 agctgagact acaagtgtgt gccacccttg cccggctaat tttgtatttt tagtagagat
     3421 gtggtttctc tgtgttaccc aggctggtcc caaaactcct ggcctcaagt gatctgcctg
     3481 ccttggcctc ccaaagtgtt aggattacag gtgtgagcca ccatgctcag cctgtagaac
     3541 ttttaaccca agtctcattt cttttttgaa agggaagagt gcacaagatt aactgcttct
     3601 ttggatgaat cattgttaat aaaaagctgg gcatttagaa ttttgcctta taagcccttc
     3661 tccaaccata agattatttt gtaccaaaaa ctttggtgtt ctctaccaaa gcagttaaaa
     3721 acttttagcc tgctacttct tgtatttgtc tactgacagc cccttggtac tatttaggtt
     3781 gggggagggg acctaaaata aatagacttt aacatttccc ttgggtgcta atcatagttg
     3841 gaagttgaat ttaaggtgat tatttgggtg acaattaaaa acctaaggaa aaccagaaat
     3901 cttggtagtg gaagaaatgt gtaaggtcac cccaatcggt agattttaat gaacgttgtg
     3961 gaatgttggg aagaggggat gttaagttga atgcagaatt tcactaagta cttagtgtaa
     4021 gtttaaggat gtagctcttt ttatctaaga attcaatgta atggccaaaa ggcagattta
     4081 ctgtttaaaa atttgaataa ttttacatga cattcttgaa attctaagaa gttttatgtg
     4141 tagaacattt taaaaattca tcagattatt aaagggaaaa taaatgatta atgataattt
     4201 tggaaggtta atgtgagcta gacttaagta aactttggtt catttgtgtt cattgaatgt
     4261 tttggaaatg accaaaaaat gtaaatggcc ttcactcaag tttgagtgtt taaagttgaa
     4321 agatgtgctc tactaaaagt tatagtaatt ctaacctcac attgaaatga gacagtattc
     4381 cttgttatac aggctgaatt tgaagattag agaggatcta atgtttactt aggtaaaggg
     4441 gcataggttt tgtagttaag atgaccagac agctaaaagc tgtgatggga agtatggact
     4501 gctcctattt atagtctcag aaaatggacc tttaggtctc tatccgtatt ggcaattatt
     4561 agaagaaagt tacacccttt tgaaactaca aaagctgtct tggaatttcc ccctcttctc
     4621 cctatttatg tccccttaga atattttagg gagcctataa ttattttcta accaaggaaa
     4681 aacttaagtc tctttaagaa gcaattactt ttcataacat cagattgaat aacccacctt
     4741 gctgttcagc ccacatccta ctggaaacaa aaggtaagaa acccattttc tggttcttga
     4801 ttgtttgggt ctgaattttg tttttaaaac taagctaagt ttaatgtttt ttaaaatgct
     4861 gtttggaata tgaatagatt ccccgtaaaa tgatttttcc taagttttat gctttagtaa
     4921 atattcagtg ctcacgtctg tgcatcatag tgcttgcgtt taatatgatt tattgtagaa
     4981 tctcaacttt tcttggtgtt tgttgtcttt gaaacattgt cttggtcatt agggctggtg
     5041 ttttcacatt tctgtggtca aggtggattt cttatgtgtg cctttttgct tactttgtat
     5101 atgaattttg taatttaaat tgcaagtaag ttatatatat gtatttacca taaatagtat
     5161 taaaagatga gaaactgtta gactgaagtt ctgttgtaac ataaccatta tttccatcac
     5221 agtatgaaga ctgcaaacgc agaaaacaga ttacagtctc ttatccattt tttgaaatcc
     5281 aaaaactacg aaaacaaaag attttctgtt gttgagctaa ttaaatgtga accctgacca
     5341 gaa
//
