LOCUS       NM_194286               8431 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens serine/arginine repetitive matrix 4 (SRRM4), mRNA.
ACCESSION   NM_194286
VERSION     NM_194286.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Labrecque,M.P., Brown,L.G., Coleman,I.M., Lakely,B., Brady,N.J.,
            Lee,J.K., Nguyen,H.M., Li,D., Hanratty,B., Haffner,M.C.,
            Rickman,D.S., True,L.D., Lin,D.W., Lam,H.M., Alumkal,J.J.,
            Corey,E., Nelson,P.S. and Morrissey,C.
  TITLE     RNA Splicing Factors SRRM3 and SRRM4 Distinguish Molecular
            Phenotypes of Castration-Resistant Neuroendocrine Prostate Cancer
  JOURNAL   Cancer Res 81 (18), 4736-4750 (2021)
   PUBMED   34312180
  REMARK    GeneRIF: RNA Splicing Factors SRRM3 and SRRM4 Distinguish Molecular
            Phenotypes of Castration-Resistant Neuroendocrine Prostate Cancer.
REFERENCE   2  (bases 1 to 8431)
  AUTHORS   Conn,V.M., Gabryelska,M., Marri,S., Stringer,B.W., Ormsby,R.J.,
            Penn,T., Poonnoose,S., Kichenadasse,G. and Conn,S.J.
  TITLE     SRRM4 Expands the Repertoire of Circular RNAs by Regulating
            Microexon Inclusion
  JOURNAL   Cells 9 (11), 2488 (2020)
   PUBMED   33207694
  REMARK    GeneRIF: SRRM4 Expands the Repertoire of Circular RNAs by
            Regulating Microexon Inclusion.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8431)
  AUTHORS   Coleman,D.J., Sampson,D.A., Sehrawat,A., Kumaraswamy,A., Sun,D.,
            Wang,Y., Schwartzman,J., Urrutia,J., Lee,A.R., Coleman,I.M.,
            Nelson,P.S., Dong,X., Morrissey,C., Corey,E., Xia,Z., Yates,J.A.
            and Alumkal,J.J.
  TITLE     Alternative splicing of LSD1+8a in neuroendocrine prostate cancer
            is mediated by SRRM4
  JOURNAL   Neoplasia 22 (6), 253-262 (2020)
   PUBMED   32403054
  REMARK    GeneRIF: Alternative splicing of LSD1+8a in neuroendocrine prostate
            cancer is mediated by SRRM4.
REFERENCE   4  (bases 1 to 8431)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 8431)
  AUTHORS   Shimojo,M., Kasahara,Y., Inoue,M., Tsunoda,S.I., Shudo,Y.,
            Kurata,T. and Obika,S.
  TITLE     A gapmer antisense oligonucleotide targeting SRRM4 is a novel
            therapeutic medicine for lung cancer
  JOURNAL   Sci Rep 9 (1), 7618 (2019)
   PUBMED   31110284
  REMARK    GeneRIF: A gapmer antisense oligonucleotide targeting SRRM4 is a
            novel therapeutic medicine for lung cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8431)
  AUTHORS   Irimia,M., Weatheritt,R.J., Ellis,J.D., Parikshak,N.N.,
            Gonatopoulos-Pournatzis,T., Babor,M., Quesnel-Vallieres,M.,
            Tapial,J., Raj,B., O'Hanlon,D., Barrios-Rodiles,M., Sternberg,M.J.,
            Cordes,S.P., Roth,F.P., Wrana,J.L., Geschwind,D.H. and
            Blencowe,B.J.
  TITLE     A highly conserved program of neuronal microexons is misregulated
            in autistic brains
  JOURNAL   Cell 159 (7), 1511-1523 (2014)
   PUBMED   25525873
  REMARK    GeneRIF: Neural microexons are regulated by the neuronal-specific
            splicing factor nSR100/SRRM4, through its binding to adjacent
            intronic enhancer motifs; neural microexons are frequently
            misregulated in the brains of individuals with autism spectrum
            disorder, and this misregulation is associated with reduced levels
            of nSR100.
REFERENCE   7  (bases 1 to 8431)
  AUTHORS   Shimojo,M., Shudo,Y., Ikeda,M., Kobashi,T. and Ito,S.
  TITLE     The small cell lung cancer-specific isoform of RE1-silencing
            transcription factor (REST) is regulated by neural-specific Ser/Arg
            repeat-related protein of 100 kDa (nSR100)
  JOURNAL   Mol Cancer Res 11 (10), 1258-1268 (2013)
   PUBMED   23928058
  REMARK    GeneRIF: findings show that nSR100 plays a role in the alternative
            splicing of REST in small cell lung cancer (SCLC); study provides
            new insight into the role of nSR100 in the expression of sREST,
            possibly in the pathogenesis of SCLC
REFERENCE   8  (bases 1 to 8431)
  AUTHORS   Comuzzie,A.G., Cole,S.A., Laston,S.L., Voruganti,V.S., Haack,K.,
            Gibbs,R.A. and Butte,N.F.
  TITLE     Novel genetic loci identified for the pathophysiology of childhood
            obesity in the Hispanic population
  JOURNAL   PLoS One 7 (12), e51954 (2012)
   PUBMED   23251661
REFERENCE   9  (bases 1 to 8431)
  AUTHORS   Calarco,J.A., Superina,S., O'Hanlon,D., Gabut,M., Raj,B., Pan,Q.,
            Skalska,U., Clarke,L., Gelinas,D., van der Kooy,D., Zhen,M.,
            Ciruna,B. and Blencowe,B.J.
  TITLE     Regulation of vertebrate nervous system alternative splicing and
            development by an SR-related protein
  JOURNAL   Cell 138 (5), 898-910 (2009)
   PUBMED   19737518
REFERENCE   10 (bases 1 to 8431)
  AUTHORS   Behrends,U., Schneider,I., Rossler,S., Frauenknecht,H., Golbeck,A.,
            Lechner,B., Eigenstetter,G., Zobywalski,C., Muller-Weihrich,S.,
            Graubner,U., Schmid,I., Sackerer,D., Spath,M., Goetz,C., Prantl,F.,
            Asmuss,H.P., Bise,K. and Mautner,J.
  TITLE     Novel tumor antigens identified by autologous antibody screening of
            childhood medulloblastoma cDNA libraries
  JOURNAL   Int J Cancer 106 (2), 244-251 (2003)
   PUBMED   12800201
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK291361.1, AB058756.1, AC084361.19 and BM680369.1.
            
            On Nov 23, 2018 this sequence version replaced NM_194286.3.
            
            Summary: SRRM4 promotes alternative splicing and inclusion of
            neural-specific exons in target mRNAs (Calarco et al., 2009 [PubMed
            19737518]).[supplied by OMIM, Oct 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC152471.1, AB058756.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000267260.5/ ENSP00000267260.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                AK291361.1         47-96
            51-1998             AB058756.1         1-1948
            1999-7991           AC084361.19        159973-165965
            7992-8431           BM680369.1         18-457              c
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.23"
     gene            1..8431
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /note="serine/arginine repetitive matrix 4"
                     /db_xref="GeneID:84530"
                     /db_xref="HGNC:HGNC:29389"
                     /db_xref="MIM:613103"
     exon            1..473
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     CDS             343..2178
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /note="neural-specific SR-related protein of 100 kDa;
                     medulloblastoma antigen MU-MB-2.76; neural-specific
                     serine/arginine repetitive splicing factor of 100 kDa"
                     /codon_start=1
                     /product="serine/arginine repetitive matrix protein 4"
                     /protein_id="NP_919262.2"
                     /db_xref="CCDS:CCDS44994.1"
                     /db_xref="GeneID:84530"
                     /db_xref="HGNC:HGNC:29389"
                     /db_xref="MIM:613103"
                     /translation="MASVQQGEKQLFEKFWRGTFKAVATPRPESIIVASITARKPLPR
                     TEPQNNPVVPAQDGPSEKLGQHLATEPLGTNSWERDKTCRELGATRGHSASHDKDLTP
                     PPSSRGKKKKKKSTRKKRRRSSSYSPSPVKKKKKKSSKKHKRRRSFSKKRRHSSSSPK
                     SKRRDEKRHKKQSRSRPRKSHRHRHHRCPSRSQSSESRPSSCESRHRGRSPEEGQKSR
                     RRHSRRCSKTLCKDSPEAQSSRPPSQPLQMLGYLSARGVITGSGSAADLFTKTASPLT
                     TSRGRSQEYDSGNDTSSPPSTQTSSARSRGQEKGSPSGGLSKSRELNSGNTSDSGNSF
                     TTSSPQNKGAMLENLSPTSRGRESRGFQSPCLECAEVKKSSLVPSTARSSPMKGCSRS
                     SSYASTRSSSHSSRSPNPRASPRYTQSRSTSSEKRSYSRSPSYSSKSGKRSPPSRSSR
                     SRRSPSYSRYSPSRERDPKYSEKDSQQRERERARRRRRSYSPMRKRRRDSPSHLEARR
                     ITSARKRPIPYYRPSPSSSGSLSSTSSWYSSSSSRSASRSYSRSRSRSRSRRRSRTRT
                     SSSSSSRSPSPGSRSRSRSRSRSRSRSRSQSRSYSSADSYSSTRR"
     exon            474..620
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            621..707
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            708..779
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            780..806
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            807..857
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            858..956
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1113
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1418
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1622
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1733
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1874
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..8431
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8403..8408
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /note="hexamer: ATTAAA"
     polyA_site      8431
                     /gene="SRRM4"
                     /gene_synonym="KIAA1853; MU-MB-2.76; nSR100"
                     /note="major polyA site"
ORIGIN      
        1 gcgcacgcac acacatccga ccccgtcgcc tcttctctcc tggtgctgcc cagaaagcca
       61 gccctccctt cccttcttgg ggcgcagagg ctcagccagc tcagagcgca gcctggagcc
      121 gacccagaag ggcgaagaaa gcccagcgga cgagcctcct ttctctgctg cctgcccggg
      181 ctggggcgtc ccatcccccg ccctgaactc cgatctctcc caccccaccc ctctctgggt
      241 ttcacccgga cagagccggg agctgggtgt cgcccccgtt tggaatccac gtttcagcac
      301 tttggacagc gccccggacg ccccggcccc tttgggttgg cgatggcgag cgttcagcaa
      361 ggcgagaagc agctttttga gaagttctgg cgaggaacct tcaaagcggt ggccaccccc
      421 cgtcccgaga gcatcattgt cgccagtatc acggcccgca agccgctgcc aaggacagag
      481 ccccagaata accccgttgt cccagctcag gatggaccct cagaaaagct gggtcagcat
      541 ctggccaccg agcccttggg caccaacagt tgggagagag acaagacctg tcgggaactg
      601 ggtgccacca gaggacacag tgcctctcat gacaaagact tgacaccacc accttcctcc
      661 aggggaaaga agaaaaagaa gaaatccact cggaagaaga gaaggaggtc ctcatcctat
      721 agcccatcgc ctgtcaagaa aaagaagaag aaaagttcca agaaacacaa gcgacgcagg
      781 tcattctcca agaagagaag gcacagctcc tctagcccaa aaagcaaaag aagagatgag
      841 aagaggcaca agaaacaatc tcgaagccgg ccccgaaagt ctcaccgcca ccgccatcac
      901 cgctgcccct cgcggtccca gagctcggag tcccgcccct caagctgtga gagcaggcac
      961 cgcggccggt cccctgagga agggcagaag tcccgccgaa ggcactcccg ccgctgctcc
     1021 aagaccctct gcaaggacag ccctgaggcc cagtccagtc gcccgcccag tcaacccctc
     1081 cagatgcttg gctacctgtc agccaggggt gtaatcactg ggtcggggtc tgctgctgac
     1141 ctctttacca aaacagccag cccgctcacc acctcgcgag gacgttccca ggagtacgac
     1201 tcaggaaatg acacgtcctc gccaccctcc acgcaaacca gctcagccag gtctcggggc
     1261 caggagaagg ggagccccag tgggggcttg agcaagagcc gggagctcaa cagtggcaac
     1321 acctctgatt cagggaactc cttcaccacc tcctcacccc agaacaaggg ggccatgttg
     1381 gagaatctct cccccaccag caggggcaga gagtcaaggg gatttcagtc accgtgtctg
     1441 gaatgtgccg aagtgaagaa gtccagtttg gtcccatcca cagcccggag ctcacccatg
     1501 aaagggtgtt cccgcagctc ctcctatgcc agcacccgat cctccagtca ctcgtcccga
     1561 tccccaaatc ccagggcttc ccccaggtac acccaaagcc gatccacctc ttctgaaaaa
     1621 aggtcctact cccgctctcc cagctattcc tccaagtctg gcaagaggag cccgcccagc
     1681 agaagctcta ggtcccgccg cagccctagc tactcccgct acagccccag cagggagcgg
     1741 gatcccaaat acagtgagaa ggactcgcag cagcgggagc gcgagcgagc gcgtcggaga
     1801 cgtcggtcct actcgcctat gagaaagcgc cggagagact ccccgagcca cctggaggcc
     1861 cggaggataa ccagtgcccg gaaacgcccc atcccctact atcggcccag cccctcctca
     1921 tccggcagcc tcagcagcac ctcctcctgg tacagcagca gcagtagccg ctcggccagc
     1981 cgcagctact cccggagccg gagtcggagc cggagccgga gacggagccg gacccgcacg
     2041 agcagcagct ctagctcccg cagccctagt ccgggctccc gcagccggag ccggagcagg
     2101 agccggagcc ggagccggag caggagccag agccggagct acagctcagc agacagctac
     2161 tccagcacga ggcgctaagt gcccctgagc cagctgcccg tgggggcccc ttcgcgctgc
     2221 cagcctcccc caaccacctg ccctccccgc cttcttggtg acaaatagtg agggctccta
     2281 taccttgtcc ttcctgcttg cctaggggaa gaggagaaga gggtaagggg gcttcactct
     2341 ctagatcagc ctgctaggag cctctaccag catcatcctg gggcccagct caggcctggg
     2401 catatggaaa gaaccatcat cttgtggcac aaaaaaagaa gaaagaaaag aaaacttcaa
     2461 ggttttgtga gaagcaatgg gtctgtgact caaaaattga gccctggcca ggaaaatgtg
     2521 gagacagttc ttctcctcca ctgctcacag gaggcccgtg gtaattctcc ccacctcccc
     2581 ggatgcccca ctagtccaac ttcatggctg cacgtggatg gacccccatg tctgagaggg
     2641 gtgaaagagg agaccaatgg cagttcaggt tcaagataac ggggtcaggc ctttaacttc
     2701 ctcaaacagg cccaagcaaa ggctggaaga catttgggtc ctagaagaag ggatatcctt
     2761 gaataccaga tggggacgtt tgacttggtg ggaggtgtca ggagatagga gttggttaag
     2821 atgatagata gaaaaacgga agggttcagc tgctgctccc aatacctcag catggtaagg
     2881 ggacagagcc cagcagatcc cagctgtgaa gaggccactg catggctgac acaaaacacc
     2941 tcttggccat tcacaggggc cctgcaacct cacgagaaag gagatagtgg aagagtcagg
     3001 acaggttggt ctccagcccc acttcccaaa tatgcctcag ccccactagc tggcaccaac
     3061 ttaattccac gggaccatct gctgaacatt ccccagtgcc atggccagtt gccaggcccc
     3121 agcgccagcc agtctggcct taccctccgg ttggcgcctg ccagccccct cccctctgcc
     3181 caggctgcca cgccctggat gccacccagt gcagcctggc acctacccac ccacccttca
     3241 gcttaagcca ctccccttgc ctctcaggaa ttttgccagg atggggcaca gcagctcagg
     3301 tttgggggaa aagacccgaa tccagagtgc aggggagagg ggcttggata tgccatgtct
     3361 tgggctcctg ctggcttctg aggtttcttg tcagcttggg aaagacccac ctttattttt
     3421 gccctcaccc caagtccccc ctggctgggg ctgggcagag agggtaagct gatctccgat
     3481 ccacaaatac caccagctgc ttcatccaca gaaggcgcta agaacgggga ggtgaggaat
     3541 aagatcctta aaataaactc ttgggcatcc ccctcaccag ctgactggct ttccggaggc
     3601 ttgtgggtga gtttcaagtt tgtggtggca acagcagcag gacagggcac ggagggagcg
     3661 agggagagag gaccggtccc agcttttgga tattttgggt tatggggaag caagggccaa
     3721 acattcttta tccctcccac cccagggaaa acatttggaa aatagtcctt tttcttggaa
     3781 gcagtgacgt ctccctaccc aatctttccc atcctatcaa cccatggggc tagagactgc
     3841 catgcaacca ccaccacgac agcccttcct ccatcagaag acaggagtga gggccccggg
     3901 cagcagtgga cgacccagcc tccccatacc gggcctctgg cccagaccta agccctgccc
     3961 acaaagacta atggatgcca gtgctgtcac tgcagaaggc cagatggcag gtgcctggga
     4021 caggggcagg tgccccgggg agggcaagta cagccactgt aaataaccct cgtccctgcc
     4081 caggaaccca gactggactt cagtctccct ccatggagac aaaggcttcc cagagccacc
     4141 aaaaaccttc ttagcaggac agctgtgaga ggcatcagaa cctcacgttc ggactgcctt
     4201 catcaagaca actctagggg accattttgc ctggggctcc tagcaaacct ttttatttat
     4261 tttatttatt tatctattta tttatttgat gactgtctct ctttctcgcc attagcagta
     4321 ttgaattatt tatttataca gaggttttgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     4381 gtgtgtgtgt gtgtgtgttg gggggatctc ctaaattatt tctgtgcatc tccttttttt
     4441 ttctgctgtt ttttatggat gggatttgag aggccaaagg tgagtgggct attggccttc
     4501 tccctgaagg ctctaagcct ggaacaccct ggggttggcc cagaaggagg cacatcagtc
     4561 ccaaaattca tcacttaacc cagcgtccag gagggtcagg cctgtacccc agcctcctgt
     4621 cccccaggaa cctcacaggg gtatcaaaaa gatgtaaaca gcccagaggc ttatgagcta
     4681 cagtgatgaa ttgacacagt ggagttgcaa cttaggtggg ggttggagtc gaaagttggc
     4741 aaatggggcc aaggctgcac aaagtcgaaa tcccttgtga atgtccctta gggaggcacc
     4801 agattggaga cagatgtgct atctcctaac agagtgatga ggcttcagtt gagtacttgc
     4861 tgaagtagtt gccttgtatt ttgagggcca tggtggatga aaatgatgtc tggtttagta
     4921 ctggaattgc acttggggtt gacaaggtgc tcacaccatg agaatggagg caggaattga
     4981 gctgactgag ggaagatgca ccttgatctc accctcactc caaggcacat agcaggatca
     5041 ctccctagct tctctgagca cacctagttg gtttcatgta tgttacacat gcttccactc
     5101 cacagtacct aatttctctc tgtcccctga gatctgaagg ctaccttggg aagaggcatc
     5161 agccatcttg cttgagaacc accaagccaa aagcaaaagc ttttatctat gatctcttgc
     5221 tgtttcatca ggggaaagca caaagctatt tctgaaatta ggaaaaaaaa aaaaaaggtg
     5281 gaaggagcag ccagatgttc cacaggaccc caccaagaag gtcatttcca aacccatcct
     5341 ggaagggccc aggatccaaa ggtcatcagt cctgcttatc tgaccaactg gcagtgtctt
     5401 ctggctgctg gccggagaca gctcttgccc tttccaaagt cactgtccac tgccttgcaa
     5461 ttgccagctt gtctggtcca gctttgggtt tggtgagact tttgcaacat ccctggttgt
     5521 ttccctggca atgtgactat ccagccctaa cccaaagcaa gggagtgccc ctttcctggg
     5581 tgaagtttac aagaaggctg cttaaatgcc tgcttcgggg aaatctctgc ctctctctct
     5641 ctctgtctct ctctctgtct ctctctctgt ctctctctct ctctctctct ctctctctct
     5701 ctcagtgtat ttctctactt tcttttacat ttcctttttt tctatccaaa aacaatgtgc
     5761 ttgttgaggc actggtaacc ctgattaacc agaacctccc attcccagta tcgctgttcc
     5821 tacgcccatt tcaccctcat taccttctgc ttccaaggaa tatgacagat caccaggatg
     5881 ctgctcgtcg tgaggattta tctcaaaaac caacatccaa aatgggaggg agatgtggct
     5941 tgaggtcaag cgccatgcat cccaagcctt tgaccttccc gctatggaag tgcactggat
     6001 gacagaaact gaatacattg ctccctttcc ctagggcaaa gttcgacctc tgttaagtgg
     6061 agggatttgt gagataaaaa ttcaaaatgt tggcctgagg cctgagagtg tcaccaaaga
     6121 cagagggagc ttcactgaga ctcagaggga aaaggaaaag agcctcaaac atttttagga
     6181 ggttgtccat catgaaagta aaaacgaaaa gcaagatttg atctcccttc agttaattag
     6241 gcaaggctaa gtaactcaaa gccccctatt agtaacattc tggttcactg aggtttgatc
     6301 atattcctat ctgcattcct tcccttcttt gaaggacagc tgatctttca gaagcagaat
     6361 aaaattaaga tgttagaaca aaggtctcag tctcagagaa ccgcatcact tcatttgctc
     6421 agacccatcc tcttttgcaa aagggtctgc ttggagaggc caaaattcag ggtgctctca
     6481 aaggcaaaga aagcacattg ttttccttct ccagtccaac tttcatcttt tcttctgctg
     6541 ttttcttttc ccctcttctt tttcacaaat gttcaaaatg gtctcatgcg catgtgtctt
     6601 gccccacttt cccctttagc tgaacagaaa attttgtctc agtaaaacga agtcaaaaaa
     6661 caggattcct ccaaacatgc ctcctcccgc actggccagc cgagtccagc tgagaaactt
     6721 atgctagatt caatgtcatt gagcaatgct ttattgaagt ctcgttcttc tcacttctgc
     6781 accagtgagc caatgatact gacagaaatg tcatctctct tctatctgtg gttgctgttt
     6841 ttggagtaaa agtttctgtg tgtgtttttt tagttctttt gatggctgtt gttttgcatt
     6901 gtaaatacca tgatggggga cccccatcag aacatggctt atttaataat ttatttcgta
     6961 tttattgagt aatattggga aaagagaagg accacctctt tccctgaatt gctattgaga
     7021 attggtccat ctcccagctc caggtgctgc tgtctgcagc aagggcatta ctgcccaggt
     7081 aaggagtgct agaatcacca agcaaattga aattggcaga aatggaggct tcagtcacac
     7141 aaattagact caaatggaac taaaacactg gttatctcca ggaaaacctc atttagatgg
     7201 aaattaatgg aagaataaaa tgcctacaca tgaaccaact tctattaaaa agtcacaact
     7261 ccttgaaaaa aaaaataaag aaaaattgta aactcttttt tttttctggc caaggaaagc
     7321 tatgcctcat cttctaacga gccaagccaa aaagactgca atggtattcc tatgtgtttc
     7381 tttggcctgt gtatcagtct gaatgaaatg gaatgggtct ctagcctcag tcttgtcatc
     7441 tgtaaaatgg ggcttgtcct atatattatc tgcaagacgt gggaaatggg ggctcaagcc
     7501 ctgatgctat ggactccata ctgttggata tattgtctct tgtgtcttct gctgactgca
     7561 gattaaaggg tgtcaaccaa ggaaggaaac aaaaaagtag ggcctggact tcatttgcag
     7621 aatgaggtca tagtcgttga gtcccacagt catatatggg agacctcaag ttgctgtcac
     7681 cttgataact cttgtatcct gggttaaagc cctctgtatt tagtttgaac ttctctctaa
     7741 gccccgtggt ccaaagtcat cacgggagag accaagatgg gcttaccttg ccctgctctg
     7801 gatttaacca ttgttcattg tcaggctata tttttgtaca atcattcaaa taacccagtg
     7861 acataggtca tattgccact tttcagagga gaaaactgag gctcaggagg gggagttgac
     7921 atgcccaagc tcccttgagc tcagatcagc ttgactcaat gtccaacatt cccttggtag
     7981 ctttttctcc ggggtcctgt gctataagaa cttctctctg cactgtattt ttttttctcc
     8041 caattcttag ctatttcctc aagcaatgat tggccaagga cctagcataa tccaccacat
     8101 tggccaaggg gacgtggtgc accccaaggc catttctctg cattggaggc tgcgaatctc
     8161 ctctggaaaa ttcccaaccc gaggacccac catgagccca gctcagcctg accagacagc
     8221 ctctgcctgg agcattcaca tcagatggaa agaagctgct gtgtcctcca gcatcctggg
     8281 accctgtcct ctgcccagtg acacagcagc catggctagc ttgatttctg gtctccaaag
     8341 ctaagcataa ccttcccggg gtttctggtt tttcagcctg tacgaaacat gtctctgttc
     8401 taattaaagt tcccatggta tggtgttctc a
//
