LOCUS       NM_014230               2831 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens signal recognition particle 68 (SRP68), transcript
            variant 1, mRNA.
ACCESSION   NM_014230
VERSION     NM_014230.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Chevalier,B., Baatallah,N., Najm,M., Castanier,S., Jung,V.,
            Pranke,I., Golec,A., Stoven,V., Marullo,S., Antigny,F.,
            Guerrera,I.C., Sermet-Gaudelus,I., Edelman,A. and Hinzpeter,A.
  TITLE     Differential CFTR-Interactome Proximity Labeling Procedures
            Identify Enrichment in Multiple SLC Transporters
  JOURNAL   Int J Mol Sci 23 (16), 8937 (2022)
   PUBMED   36012204
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2831)
  AUTHORS   Lim,S.H., Snider,J., Birimberg-Schwartz,L., Ip,W., Serralha,J.C.,
            Botelho,H.M., Lopes-Pacheco,M., Pinto,M.C., Moutaoufik,M.T.,
            Zilocchi,M., Laselva,O., Esmaeili,M., Kotlyar,M., Lyakisheva,A.,
            Tang,P., Lopez Vazquez,L., Akula,I., Aboualizadeh,F., Wong,V.,
            Grozavu,I., Opacak-Bernardi,T., Yao,Z., Mendoza,M., Babu,M.,
            Jurisica,I., Gonska,T., Bear,C.E., Amaral,M.D. and Stagljar,I.
  TITLE     CFTR interactome mapping using the mammalian membrane two-hybrid
            high-throughput screening system
  JOURNAL   Mol Syst Biol 18 (2), e10629 (2022)
   PUBMED   35156780
REFERENCE   4  (bases 1 to 2831)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2831)
  AUTHORS   Menichelli,E., Isel,C., Oubridge,C. and Nagai,K.
  TITLE     Protein-induced conformational changes of RNA during the assembly
            of human signal recognition particle
  JOURNAL   J Mol Biol 367 (1), 187-203 (2007)
   PUBMED   17254600
REFERENCE   6  (bases 1 to 2831)
  AUTHORS   Iakhiaeva,E., Bhuiyan,S.H., Yin,J. and Zwieb,C.
  TITLE     Protein SRP68 of human signal recognition particle: identification
            of the RNA and SRP72 binding domains
  JOURNAL   Protein Sci 15 (6), 1290-1302 (2006)
   PUBMED   16672232
  REMARK    GeneRIF: The RNA binding domain of SRP68 included residues from
            positions 52 to 252. Ninety-four amino acids near the C terminus of
            SRP68 mediated the binding to SRP72
REFERENCE   7  (bases 1 to 2831)
  AUTHORS   Harada,H., Nagai,H., Mine,N., Terada,Y., Fujiwara,H., Mikami,I.,
            Tsuneizumi,M., Yabe,A., Miyazaki,K., Yokota,T., Imoto,I.,
            Inazawa,J. and Emi,M.
  TITLE     Molecular cloning, tissue expression, and chromosomal assignment of
            a novel gene encoding a subunit of the human signal-recognition
            particle
  JOURNAL   J Hum Genet 46 (2), 70-75 (2001)
   PUBMED   11281415
REFERENCE   8  (bases 1 to 2831)
  AUTHORS   Politz,J.C., Yarovoi,S., Kilroy,S.M., Gowda,K., Zwieb,C. and
            Pederson,T.
  TITLE     Signal recognition particle components in the nucleolus
  JOURNAL   Proc Natl Acad Sci U S A 97 (1), 55-60 (2000)
   PUBMED   10618370
REFERENCE   9  (bases 1 to 2831)
  AUTHORS   Larsen,N., Samuelsson,T. and Zwieb,C.
  TITLE     The Signal Recognition Particle Database (SRPDB)
  JOURNAL   Nucleic Acids Res 26 (1), 177-178 (1998)
   PUBMED   9399828
REFERENCE   10 (bases 1 to 2831)
  AUTHORS   Lutcke,H., Prehn,S., Ashford,A.J., Remus,M., Frank,R. and
            Dobberstein,B.
  TITLE     Assembly of the 68- and 72-kD proteins of signal recognition
            particle with 7S RNA
  JOURNAL   J Cell Biol 121 (5), 977-985 (1993)
   PUBMED   8388879
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AU137443.1, AK074698.1,
            AF195951.1, DQ786315.1 and DB315535.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014230.3.
            
            Summary: This gene encodes a subunit of the signal recognition
            particle (SRP). The SRP is a ribonucleoprotein complex that
            transports secreted and membrane proteins to the endoplasmic
            reticulum for processing. The complex includes a 7S RNA and six
            protein subunits. The encoded protein is the 68kDa component of the
            SRP, and forms a heterodimer with the 72kDa subunit that is
            required for SRP function. Alternatively spliced transcript
            variants encoding multiple isoforms have been observed for this
            gene, and three pseudogenes of this gene are located within the
            Smith-Magenis syndrome region on chromosome 17. [provided by
            RefSeq, May 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1063095.1,
                                           SRR1803617.1913.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000307877.7/ ENSP00000312066.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AU137443.1         10-24
            16-838              AK074698.1         1-823
            839-1160            AF195951.1         789-1110
            1161-2153           AK074698.1         1146-2138
            2154-2510           DQ786315.1         734-1090
            2511-2600           DB315535.1         243-332             c
            2601-2831           DB315535.1         11-241              c
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.1"
     gene            1..2831
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /note="signal recognition particle 68"
                     /db_xref="GeneID:6730"
                     /db_xref="HGNC:HGNC:11302"
                     /db_xref="MIM:604858"
     exon            1..210
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..1910
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /note="isoform 1 is encoded by transcript variant 1;
                     signal recognition particle 68 kDa protein; signal
                     recognition particle 68kDa; signal recognition particle
                     subunit SRP68"
                     /codon_start=1
                     /product="signal recognition particle subunit SRP68
                     isoform 1"
                     /protein_id="NP_055045.2"
                     /db_xref="CCDS:CCDS11738.1"
                     /db_xref="GeneID:6730"
                     /db_xref="HGNC:HGNC:11302"
                     /db_xref="MIM:604858"
                     /translation="MAAEKQVPGGGGGGGSGGGGGSGGGGSGGGRGAGGEENKENERP
                     SAGSKANKEFGDSLSLEILQIIKESQQQHGLRHGDFQRYRGYCSRRQRRLRKTLNFKM
                     GNRHKFTGKKVTEELLTDNRYLLLVLMDAERAWSYAMQLKQEANTEPRKRFHLLSRLR
                     KAVKHAEELERLCESNRVDAKTKLEAQAYTAYLSGMLRFEHQEWKAAIEAFNKCKTIY
                     EKLASAFTEEQAVLYNQRVEEISPNIRYCAYNIGDQSAINELMQMRLRSGGTEGLLAE
                     KLEALITQTRAKQAATMSEVEWRGRTVPVKIDKVRIFLLGLADNEAAIVQAESEETKE
                     RLFESMLSECRDAIQVVREELKPDQKQRDYILEGEPGKVSNLQYLHSYLTYIKLSTAI
                     KRNENMAKGLQRALLQQQPEDDSKRSPRPQDLIRLYDIILQNLVELLQLPGLEEDKAF
                     QKEIGLKTLVFKAYRCFFIAQSYVLVKKWSEALVLYDRVLKYANEVNSDAGAFKNSLK
                     DLPDVQELITQVRSEKCSLQAAAILDANDAHQTETSSSQVKDNKPLVERFETFCLDPS
                     LVTKQANLVHFPPGFQPIPCKPLFFDLALNHVAFPPLEDKLEQKTKSGLTGYIKGIFG
                     FRS"
     exon            211..277
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            278..391
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            392..587
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            588..670
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            671..780
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            781..863
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            864..1004
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1103
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1168
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1325
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1420
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1550
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1626
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1682
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..2831
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2049
                     /gene="SRP68"
                     /gene_synonym="SCN10"
     regulatory      2490..2495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /note="hexamer: ATTAAA"
     polyA_site      2513
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /note="major polyA site"
     regulatory      2809..2814
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SRP68"
                     /gene_synonym="SCN10"
                     /note="hexamer: AATAAA"
     polyA_site      2831
                     /gene="SRP68"
                     /gene_synonym="SCN10"
ORIGIN      
        1 gtcttgctct gcggcgcagg ggcaagatgg ctgctgagaa gcaggtccca ggcggcggcg
       61 gcggcggcgg cagtggcggc ggcggtggca gtggcggcgg cggtagcggc ggtggacgtg
      121 gtgccggagg ggaagaaaat aaagaaaacg aacgcccttc ggccggatcg aaggcaaaca
      181 aagaatttgg ggatagcctg agtttggaga ttcttcagat tattaaggaa tcccagcagc
      241 agcatggttt acggcatgga gattttcaga ggtacagggg ctactgttcc cgtagacaaa
      301 gacgtcttcg aaaaacactc aacttcaaga tgggtaacag acacaaattc acagggaaga
      361 aagtgactga agagcttctg accgataata gatacttgct tctggttctg atggatgctg
      421 aaagagcctg gagctacgcc atgcagctga aacaggaagc caacactgaa ccccgaaaac
      481 ggtttcactt gttatctcgc ctacgcaaag ccgtgaagca tgcagaggaa ttggaacgct
      541 tgtgtgagag caatcgcgtg gatgccaaga ccaaattaga ggctcaggct tacacagctt
      601 acctctcagg aatgctacgt tttgaacatc aagaatggaa agctgccatt gaggctttta
      661 acaaatgcaa aactatctat gagaagctag ccagtgcttt cacagaggag caggctgtgc
      721 tgtataacca acgtgtggaa gagatttcac ccaacatccg ctattgtgca tataatattg
      781 gggaccagtc agccatcaat gaactcatgc agatgagatt gaggtctggg ggcactgagg
      841 gtctcttggc tgaaaaattg gaggctttga tcactcagac tcgagccaaa caggcagcta
      901 ccatgagtga agtggagtgg agagggagaa cggttccagt gaagattgac aaagtgcgca
      961 ttttcttatt aggactggct gataacgaag cagctattgt ccaggctgaa agcgaagaaa
     1021 ctaaggagcg cctgtttgaa tcaatgctca gcgagtgtcg ggacgccatc caggtggttc
     1081 gggaggagct caagccagat cagaaacaga gagattatat ccttgaagga gagccaggga
     1141 aggtgtctaa tcttcaatac ttgcatagct acctgactta catcaagcta tcaacggcaa
     1201 tcaagcgtaa tgagaacatg gccaaaggtc tgcagagggc tctgctgcag cagcagccag
     1261 aggatgacag caagcgctca ccccggcccc aggacctgat ccgactctat gacatcatct
     1321 tacagaatct ggtggaattg ctccagcttc ctggtttaga ggaagacaaa gccttccaga
     1381 aagagatagg cctcaagact ctggtgttca aagcttacag gtgttttttc attgctcagt
     1441 cctatgtgct ggtgaagaag tggagcgaag cccttgtcct gtatgacaga gtcctgaaat
     1501 atgcaaatga agtaaattct gatgctggcg ccttcaagaa cagcctaaag gacctgcctg
     1561 atgtgcaaga gctcatcact caagtgcggt cagagaagtg ctccctgcag gccgcagcca
     1621 tccttgatgc aaacgacgct catcaaacag agacctcctc ctcccaagtc aaggacaata
     1681 agcctctggt tgaacggttt gagacattct gcctggaccc ttcccttgtc accaagcaag
     1741 ccaaccttgt gcacttccca ccaggcttcc agcccattcc ctgcaagcct ttgttctttg
     1801 acctggccct caaccatgtg gctttcccac cccttgagga caagttggaa cagaagacca
     1861 agagtggcct cactggatac atcaagggca tctttggatt caggagctaa ccaggctctt
     1921 cctcgggggc gggggagatt ctgactctta atctgtattg tgagaaaatc ccagcaagtt
     1981 ccatgatatt aaatccaggt ctgcattggc ccggggcaag agtttaacat cttcggccct
     2041 gcattcctac atcttgtgtc tgtacacgtt cttaagcagc gtgtcaggag agcaccctgt
     2101 tgtcttctgg taaatgtgtg cagggtcatc ctgtctcctg tacctcctgg gaaaggggcc
     2161 gctgctgtct ggtgccctgt gagctgtgat tgattgcctt tggtcagtaa tgcgttcagg
     2221 agtccacacc aggcacagat ggggccttga aacgctttgt catgcttctt cagtaccatg
     2281 gatttgaaat gaactcatcc ttgctgtgag catccaggag cccttgagaa gtttatctat
     2341 gactatgaaa ctggcaacgt caccccagaa ttacggtcag ccttattccc cttcacctcc
     2401 cagtgaacgc taagaagttt cagacaagca gagagctcta tttttagaag aaatatgtta
     2461 cactcagaaa tgatgaaacc aaatcttata ttaaaaggca aagatgacgg agactgtgcc
     2521 catttcttat atgccctccc tcatgtccag tccccgttct ctcctcggga gcctagttgc
     2581 gtgaagccgg tgaggtcaag tgtaacctga cttaccggca actaggtgag gctgatgcca
     2641 gatacacatg ttagaggcac tatttttcag gacttcccaa tgtgtaattt ttagatgcca
     2701 ttatatttta atccccttcg ttaccccccg tttttcctta gtcatccctt ttcacttcta
     2761 ttataacatc aataatagaa gtcacaaaaa caatgtaaga aagcaaggaa taaaagtgat
     2821 ttaaacatgt a
//
