LOCUS       NM_194285               5601 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens SPT2 chromatin protein domain containing 1 (SPTY2D1),
            mRNA.
ACCESSION   NM_194285 XM_084736
VERSION     NM_194285.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Yin,L., Tang,Y., Xiao,M., Li,M., Huang Fu,Z.M. and Wang,Y.L.
  TITLE     The role of histone chaperone spty2d1 in human colorectal cancer
  JOURNAL   Mol Cell Probes 64, 101832 (2022)
   PUBMED   35691597
  REMARK    GeneRIF: The role of histone chaperone spty2d1 in human colorectal
            cancer.
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 5601)
  AUTHORS   Guo,T., Yin,R.X., Bin,Y., Nie,R.J., Chen,X. and Pan,S.L.
  TITLE     Association of the SPT2 chromatin protein domain containing 1 gene
            rs17579600 polymorphism and serum lipid traits
  JOURNAL   Int J Clin Exp Pathol 8 (10), 12995-13010 (2015)
   PUBMED   26722495
  REMARK    GeneRIF: there may be a racial/ethnic- and/or sex-specific
            association between the SPTY2D1 rs17579600 single nucleotide
            polymorphism and serum lipid parameters in some ethnic groups.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5601)
  AUTHORS   Hasegawa,Y., Taylor,D., Ovchinnikov,D.A., Wolvetang,E.J., de
            Torrente,L. and Mar,J.C.
  TITLE     Variability of Gene Expression Identifies Transcriptional
            Regulators of Early Human Embryonic Development
  JOURNAL   PLoS Genet 11 (8), e1005428 (2015)
   PUBMED   26288249
  REMARK    GeneRIF: Results from a study on gene expression variability
            markers in early-stage human embryos shows that SPTY2D1 is a
            putative expression variability marker for the 3-day, 8-cell embryo
            stage.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5601)
  AUTHORS   Chen,S., Rufiange,A., Huang,H., Rajashankar,K.R., Nourani,A. and
            Patel,D.J.
  TITLE     Structure-function studies of histone H3/H4 tetramer maintenance
            during transcription by chaperone Spt2
  JOURNAL   Genes Dev 29 (12), 1326-1340 (2015)
   PUBMED   26109053
REFERENCE   6  (bases 1 to 5601)
  AUTHORS   Guo,T., Yin,R.X., Chen,X., Bin,Y., Nie,R.J. and Li,H.
  TITLE     Sex-specific association of the SPTY2D1 rs7934205 polymorphism and
            serum lipid levels
  JOURNAL   Int J Clin Exp Pathol 8 (1), 665-681 (2015)
   PUBMED   25755761
  REMARK    GeneRIF: These findings suggest that the association between the
            SPTY2D1 rs7934205 SNP and serum lipid levels might have ethnic-
            and/or sex-specificity.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5601)
  AUTHORS   Willer,C.J., Schmidt,E.M., Sengupta,S., Peloso,G.M., Gustafsson,S.,
            Kanoni,S., Ganna,A., Chen,J., Buchkovich,M.L., Mora,S.,
            Beckmann,J.S., Bragg-Gresham,J.L., Chang,H.Y., Demirkan,A., Den
            Hertog,H.M., Do,R., Donnelly,L.A., Ehret,G.B., Esko,T.,
            Feitosa,M.F., Ferreira,T., Fischer,K., Fontanillas,P., Fraser,R.M.,
            Freitag,D.F., Gurdasani,D., Heikkila,K., Hypponen,E., Isaacs,A.,
            Jackson,A.U., Johansson,A., Johnson,T., Kaakinen,M., Kettunen,J.,
            Kleber,M.E., Li,X., Luan,J., Lyytikainen,L.P., Magnusson,P.K.E.,
            Mangino,M., Mihailov,E., Montasser,M.E., Muller-Nurasyid,M.,
            Nolte,I.M., O'Connell,J.R., Palmer,C.D., Perola,M., Petersen,A.K.,
            Sanna,S., Saxena,R., Service,S.K., Shah,S., Shungin,D., Sidore,C.,
            Song,C., Strawbridge,R.J., Surakka,I., Tanaka,T., Teslovich,T.M.,
            Thorleifsson,G., Van den Herik,E.G., Voight,B.F., Volcik,K.A.,
            Waite,L.L., Wong,A., Wu,Y., Zhang,W., Absher,D., Asiki,G.,
            Barroso,I., Been,L.F., Bolton,J.L., Bonnycastle,L.L., Brambilla,P.,
            Burnett,M.S., Cesana,G., Dimitriou,M., Doney,A.S.F., Doring,A.,
            Elliott,P., Epstein,S.E., Ingi Eyjolfsson,G., Gigante,B.,
            Goodarzi,M.O., Grallert,H., Gravito,M.L., Groves,C.J., Hallmans,G.,
            Hartikainen,A.L., Hayward,C., Hernandez,D., Hicks,A.A., Holm,H.,
            Hung,Y.J., Illig,T., Jones,M.R., Kaleebu,P., Kastelein,J.J.P.,
            Khaw,K.T., Kim,E., Klopp,N., Komulainen,P., Kumari,M.,
            Langenberg,C., Lehtimaki,T., Lin,S.Y., Lindstrom,J., Loos,R.J.F.,
            Mach,F., McArdle,W.L., Meisinger,C., Mitchell,B.D., Muller,G.,
            Nagaraja,R., Narisu,N., Nieminen,T.V.M., Nsubuga,R.N., Olafsson,I.,
            Ong,K.K., Palotie,A., Papamarkou,T., Pomilla,C., Pouta,A.,
            Rader,D.J., Reilly,M.P., Ridker,P.M., Rivadeneira,F., Rudan,I.,
            Ruokonen,A., Samani,N., Scharnagl,H., Seeley,J., Silander,K.,
            Stancakova,A., Stirrups,K., Swift,A.J., Tiret,L.,
            Uitterlinden,A.G., van Pelt,L.J., Vedantam,S., Wainwright,N.,
            Wijmenga,C., Wild,S.H., Willemsen,G., Wilsgaard,T., Wilson,J.F.,
            Young,E.H., Zhao,J.H., Adair,L.S., Arveiler,D., Assimes,T.L.,
            Bandinelli,S., Bennett,F., Bochud,M., Boehm,B.O., Boomsma,D.I.,
            Borecki,I.B., Bornstein,S.R., Bovet,P., Burnier,M., Campbell,H.,
            Chakravarti,A., Chambers,J.C., Chen,Y.I., Collins,F.S.,
            Cooper,R.S., Danesh,J., Dedoussis,G., de Faire,U., Feranil,A.B.,
            Ferrieres,J., Ferrucci,L., Freimer,N.B., Gieger,C., Groop,L.C.,
            Gudnason,V., Gyllensten,U., Hamsten,A., Harris,T.B., Hingorani,A.,
            Hirschhorn,J.N., Hofman,A., Hovingh,G.K., Hsiung,C.A.,
            Humphries,S.E., Hunt,S.C., Hveem,K., Iribarren,C., Jarvelin,M.R.,
            Jula,A., Kahonen,M., Kaprio,J., Kesaniemi,A., Kivimaki,M.,
            Kooner,J.S., Koudstaal,P.J., Krauss,R.M., Kuh,D., Kuusisto,J.,
            Kyvik,K.O., Laakso,M., Lakka,T.A., Lind,L., Lindgren,C.M.,
            Martin,N.G., Marz,W., McCarthy,M.I., McKenzie,C.A., Meneton,P.,
            Metspalu,A., Moilanen,L., Morris,A.D., Munroe,P.B., Njolstad,I.,
            Pedersen,N.L., Power,C., Pramstaller,P.P., Price,J.F., Psaty,B.M.,
            Quertermous,T., Rauramaa,R., Saleheen,D., Salomaa,V.,
            Sanghera,D.K., Saramies,J., Schwarz,P.E.H., Sheu,W.H.,
            Shuldiner,A.R., Siegbahn,A., Spector,T.D., Stefansson,K.,
            Strachan,D.P., Tayo,B.O., Tremoli,E., Tuomilehto,J., Uusitupa,M.,
            van Duijn,C.M., Vollenweider,P., Wallentin,L., Wareham,N.J.,
            Whitfield,J.B., Wolffenbuttel,B.H.R., Ordovas,J.M., Boerwinkle,E.,
            Palmer,C.N.A., Thorsteinsdottir,U., Chasman,D.I., Rotter,J.I.,
            Franks,P.W., Ripatti,S., Cupples,L.A., Sandhu,M.S., Rich,S.S.,
            Boehnke,M., Deloukas,P., Kathiresan,S., Mohlke,K.L., Ingelsson,E.
            and Abecasis,G.R.
  CONSRTM   Global Lipids Genetics Consortium
  TITLE     Discovery and refinement of loci associated with lipid levels
  JOURNAL   Nat Genet 45 (11), 1274-1283 (2013)
   PUBMED   24097068
REFERENCE   8  (bases 1 to 5601)
  AUTHORS   Osakabe,A., Tachiwana,H., Takaku,M., Hori,T., Obuse,C., Kimura,H.,
            Fukagawa,T. and Kurumizaka,H.
  TITLE     Vertebrate Spt2 is a novel nucleolar histone chaperone that assists
            in ribosomal DNA transcription
  JOURNAL   J Cell Sci 126 (Pt 6), 1323-1332 (2013)
   PUBMED   23378026
  REMARK    GeneRIF: Spt2 is a novel histone chaperone with a separate
            DNA-binding domain that facilitates ribosomal DNA transcription
            through chromatin remodeling during transcription.
REFERENCE   9  (bases 1 to 5601)
  AUTHORS   Teslovich,T.M., Musunuru,K., Smith,A.V., Edmondson,A.C.,
            Stylianou,I.M., Koseki,M., Pirruccello,J.P., Ripatti,S.,
            Chasman,D.I., Willer,C.J., Johansen,C.T., Fouchier,S.W., Isaacs,A.,
            Peloso,G.M., Barbalic,M., Ricketts,S.L., Bis,J.C., Aulchenko,Y.S.,
            Thorleifsson,G., Feitosa,M.F., Chambers,J., Orho-Melander,M.,
            Melander,O., Johnson,T., Li,X., Guo,X., Li,M., Shin Cho,Y., Jin
            Go,M., Jin Kim,Y., Lee,J.Y., Park,T., Kim,K., Sim,X., Twee-Hee
            Ong,R., Croteau-Chonka,D.C., Lange,L.A., Smith,J.D., Song,K., Hua
            Zhao,J., Yuan,X., Luan,J., Lamina,C., Ziegler,A., Zhang,W.,
            Zee,R.Y., Wright,A.F., Witteman,J.C., Wilson,J.F., Willemsen,G.,
            Wichmann,H.E., Whitfield,J.B., Waterworth,D.M., Wareham,N.J.,
            Waeber,G., Vollenweider,P., Voight,B.F., Vitart,V.,
            Uitterlinden,A.G., Uda,M., Tuomilehto,J., Thompson,J.R., Tanaka,T.,
            Surakka,I., Stringham,H.M., Spector,T.D., Soranzo,N., Smit,J.H.,
            Sinisalo,J., Silander,K., Sijbrands,E.J., Scuteri,A., Scott,J.,
            Schlessinger,D., Sanna,S., Salomaa,V., Saharinen,J., Sabatti,C.,
            Ruokonen,A., Rudan,I., Rose,L.M., Roberts,R., Rieder,M.,
            Psaty,B.M., Pramstaller,P.P., Pichler,I., Perola,M., Penninx,B.W.,
            Pedersen,N.L., Pattaro,C., Parker,A.N., Pare,G., Oostra,B.A.,
            O'Donnell,C.J., Nieminen,M.S., Nickerson,D.A., Montgomery,G.W.,
            Meitinger,T., McPherson,R., McCarthy,M.I., McArdle,W., Masson,D.,
            Martin,N.G., Marroni,F., Mangino,M., Magnusson,P.K., Lucas,G.,
            Luben,R., Loos,R.J., Lokki,M.L., Lettre,G., Langenberg,C.,
            Launer,L.J., Lakatta,E.G., Laaksonen,R., Kyvik,K.O., Kronenberg,F.,
            Konig,I.R., Khaw,K.T., Kaprio,J., Kaplan,L.M., Johansson,A.,
            Jarvelin,M.R., Janssens,A.C., Ingelsson,E., Igl,W., Kees
            Hovingh,G., Hottenga,J.J., Hofman,A., Hicks,A.A., Hengstenberg,C.,
            Heid,I.M., Hayward,C., Havulinna,A.S., Hastie,N.D., Harris,T.B.,
            Haritunians,T., Hall,A.S., Gyllensten,U., Guiducci,C., Groop,L.C.,
            Gonzalez,E., Gieger,C., Freimer,N.B., Ferrucci,L., Erdmann,J.,
            Elliott,P., Ejebe,K.G., Doring,A., Dominiczak,A.F., Demissie,S.,
            Deloukas,P., de Geus,E.J., de Faire,U., Crawford,G., Collins,F.S.,
            Chen,Y.D., Caulfield,M.J., Campbell,H., Burtt,N.P.,
            Bonnycastle,L.L., Boomsma,D.I., Boekholdt,S.M., Bergman,R.N.,
            Barroso,I., Bandinelli,S., Ballantyne,C.M., Assimes,T.L.,
            Quertermous,T., Altshuler,D., Seielstad,M., Wong,T.Y., Tai,E.S.,
            Feranil,A.B., Kuzawa,C.W., Adair,L.S., Taylor,H.A. Jr.,
            Borecki,I.B., Gabriel,S.B., Wilson,J.G., Holm,H.,
            Thorsteinsdottir,U., Gudnason,V., Krauss,R.M., Mohlke,K.L.,
            Ordovas,J.M., Munroe,P.B., Kooner,J.S., Tall,A.R., Hegele,R.A.,
            Kastelein,J.J., Schadt,E.E., Rotter,J.I., Boerwinkle,E.,
            Strachan,D.P., Mooser,V., Stefansson,K., Reilly,M.P., Samani,N.J.,
            Schunkert,H., Cupples,L.A., Sandhu,M.S., Ridker,P.M., Rader,D.J.,
            van Duijn,C.M., Peltonen,L., Abecasis,G.R., Boehnke,M. and
            Kathiresan,S.
  TITLE     Biological, clinical and population relevance of 95 loci for blood
            lipids
  JOURNAL   Nature 466 (7307), 707-713 (2010)
   PUBMED   20686565
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX647798.1, BC056261.1, BX641102.1, BX648478.1, BC036844.1,
            AK096760.1, AF452716.1, BX648114.1, BX538046.1, AL834393.1 and
            AF086471.1.
            
            On Nov 23, 2018 this sequence version replaced NM_194285.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647798.1, SRR1660807.173185.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467146, SAMEA2467148
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000336349.6/ ENSP00000337991.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-94                BX647798.1         132-225
            95-404              BC056261.1         104-413
            405-449             BX641102.1         437-481
            450-1052            BC056261.1         456-1058
            1053-1193           BX647798.1         1184-1324
            1194-1206           BX648478.1         1247-1259
            1207-1333           BX647798.1         1338-1464
            1334-1934           BC036844.1         85-685
            1935-1965           AK096760.1         2086-2116
            1966-2623           BC036844.1         717-1374
            2624-2665           AF452716.1         2111-2152
            2666-2679           BX647798.1         2797-2810
            2680-2716           AF452716.1         2166-2202
            2717-3247           BX648114.1         2427-2957
            3248-3318           BX648478.1         3300-3370
            3319-3414           BX641102.1         3351-3446
            3415-3787           BX647798.1         3546-3918
            3788-4129           BX538046.1         2298-2639
            4130-4295           AL834393.1         2311-2476
            4296-4874           AL834393.1         2478-3056
            4875-5132           BX538046.1         3385-3642
            5133-5135           AF086471.1         140-142
            5136-5601           AF086471.1         144-609
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.1"
     gene            1..5601
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /note="SPT2 chromatin protein domain containing 1"
                     /db_xref="GeneID:144108"
                     /db_xref="HGNC:HGNC:26818"
     exon            1..145
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..2143
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /note="SPT2 domain-containing protein 1; SPT2, Suppressor
                     of Ty, domain containing 1"
                     /codon_start=1
                     /product="protein SPT2 homolog"
                     /protein_id="NP_919261.2"
                     /db_xref="CCDS:CCDS31441.1"
                     /db_xref="GeneID:144108"
                     /db_xref="HGNC:HGNC:26818"
                     /translation="MDFREILMIASKGQGVNNVPKRYSLAVGPPKKDPKVKGVQSAAV
                     QAFLKRKEEELRRKALEEKRRKEELVKKRIELKHDKKARAMAKRTKDNFHGYNGIPIE
                     EKSKKRQATESHTSQGTDREYEMEEENEFLEYNHAESEQEYEEEQEPPKVESKPKVPL
                     KSAPPPMNFTDLLRLAEKKQFEPVEIKVVKKSEERPMTAEELREREFLERKHRRKKLE
                     TDGKLPPTVSKKAPSQKESVGTKLSKGSGDRHPSSKGMPLPHAEKKSRPSMANEKHLA
                     LSSSKSMPGERIKAGSGNSSQPSLREGHDKPVFNGAGKPHSSTSSPSVPKTSASRTQK
                     SAVEHKAKKSLSHPSHSRPGPMVTPHNKAKSPGVRQPGSSSSSAPGQPSTGVARPTVS
                     SGPVPRRQNGSSSSGPERSISGSKKPTNDSNPSRRTVSGTCGPGQPASSSGGPGRPIS
                     GSVSSARPLGSSRGPGRPVSSPHELRRPVSGLGPPGRSVSGPGRSISGSIPAGRTVSN
                     SVPGRPVSSLGPGQTVSSSGPTIKPKCTVVSETISSKNIISRSSNGQMNGMKPPLSGY
                     RAAQGPQRLPFPTGYKRQREYEEEDDDDDEYDSEMEDFIEDEGEPQEEISKHIREIFG
                     YDRKKYKDESDYALRYMESSWKEQQKEEAKSLRLGMQEDLEEMRREEEEMQRRRAKKL
                     KRR"
     exon            146..260
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            261..1796
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1971
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1972..2049
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..5601
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2709..2714
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /note="hexamer: AATAAA"
     polyA_site      2729
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /note="major polyA site"
     regulatory      4436..4441
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /note="hexamer: ATTAAA"
     polyA_site      4459
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /note="major polyA site"
     regulatory      5583..5588
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
                     /note="hexamer: ATTAAA"
     polyA_site      5601
                     /gene="SPTY2D1"
                     /gene_synonym="Spt2"
ORIGIN      
        1 agttggctgt agctgggcgc ctcggttagg tgcgctgtca gtcctttccg agtgcctggc
       61 ccagtctctc ccgcctcggc ccaacatgga cttcagagaa attctcatga tagcttccaa
      121 gggacaaggt gtcaacaatg tgccgaaaag gtatagtttg gcagtggggc ctccaaaaaa
      181 agacccaaaa gttaaaggtg tccaatcagc agctgtacaa gcttttctta aaaggaaaga
      241 agaggagctg agacgaaaag ccttagagga gaaaaggaga aaagaggaac tagtgaaaaa
      301 gcgaattgag ctcaaacatg acaagaaagc aagagctatg gccaagagga caaaggataa
      361 tttccatggt tacaatggga ttcctattga ggaaaagtca aagaagaggc aggcaacaga
      421 aagccatacc agccaaggaa ccgaccgaga gtatgaaatg gaagaagaga atgaattcct
      481 cgagtacaat cacgcagagt cagagcagga gtatgaggaa gagcaagaac ctcccaaagt
      541 tgaaagcaaa ccaaaggtcc cccttaaaag tgccccacca cccatgaact tcactgattt
      601 actcaggctg gctgagaaaa agcagtttga accagtggaa atcaaggtag tgaagaaatc
      661 agaagagcga cctatgaccg cagaagaact tagggagcga gaattccttg aacgaaagca
      721 taggagaaaa aaacttgaga cagatggaaa actacctcca actgtgtcca aaaaggcacc
      781 ctctcagaaa gaaagtgtgg gcacaaaact tagcaaaggt tctggagaca ggcatccttc
      841 ttccaaagga atgccccttc ctcatgctga gaagaaatca agacccagca tggccaatga
      901 gaaacacctc gctttgtctt catccaaatc catgccagga gagaggatca aggcaggatc
      961 tggcaatagc tcccaaccct cacttcgtga gggccacgac aaacctgttt ttaatggagc
     1021 tggaaagcct cattccagca cctcttcacc aagtgtccca aagacttctg ctagcaggac
     1081 tcagaaatct gctgttgagc acaaagccaa aaaatctctg tcccatccta gccattccag
     1141 gcctgggccc atggtcaccc cacacaataa ggctaagagt ccaggtgtca ggcagccagg
     1201 cagcagctct agctcagccc ctgggcagcc cagcacaggg gttgctcgac ccacagttag
     1261 ttctggccct gtgcctaggc gccagaatgg cagctccagc tcaggacctg agcgatcaat
     1321 cagtgggtcc aagaagccaa ccaatgactc aaatccctct aggcggacag tcagtggtac
     1381 atgtggccct ggacaacctg caagcagctc aggtggccct gggcgaccca tcagtggttc
     1441 agttagttct gcaagaccct tgggcagctc tcgtggccct ggccggcctg tgagcagtcc
     1501 acatgaactt cgacgaccag tgagtggctt gggccccccg gggcggtctg tcagtggccc
     1561 tgggagatcc ataagtggct caattccagc tggacggact gtcagtaatt cagtcccagg
     1621 aagaccagtg agcagcttgg gacctgggca aacagttagt agctcaggtc ccactataaa
     1681 gcctaagtgc actgttgtct ccgaaacaat ttcttccaag aatatcatta gccggtccag
     1741 caatggacag atgaatggaa tgaagcctcc cctatctggc tacagagctg cccaaggtcc
     1801 tcaaaggctt cccttcccta ctggttacaa aaggcagcga gaatatgaag aggaagatga
     1861 cgatgatgat gaatacgact ctgaaatgga agattttatt gaagatgaag gagagcctca
     1921 ggaagaaata tccaagcaca ttagagaaat ctttggttat gaccgaaaaa aatacaaaga
     1981 tgaaagtgat tatgccttac gttacatgga aagtagttgg aaagagcagc agaaggaaga
     2041 agcaaagagc ttaagactgg gtatgcaaga ggacttagag gaaatgagac gtgaagaaga
     2101 agaaatgcaa cgtcgaaggg ccaagaagct gaagaggcgt tagctgctgc ttttattttt
     2161 gtgaaattct agagctgctc tgctgcaagg atttcctgcc ttcagactga agaagccccc
     2221 ttatcatttt agatttatac ttgggtactc aaggagaagg aatgcatact gtcatttgca
     2281 ggctgtcatt tgagcataaa atattttcag atattttcca gggtgaaatc tgtaatgcag
     2341 atgatataat gcccacttgt gctcttatga agttaactgt tcacagatgc cagccttgat
     2401 cctgatgagc tatgcactct aatgtggggc cactcaccag tcagccaatc ttttattgtt
     2461 cctttgctac tttgaaagga aaaaaaaggt tgtctcttga tttctgtaat tcttctgctg
     2521 atctgtgagt agggcactgc cctgctccca atcaaactta gtctttttat gagaaacctg
     2581 gaccttccat gaatgttgcc agcaataaag tgacttgtcc taatctatgg attgagaact
     2641 tgaaaatgta gagtagaatt tataactatc atatggatgt catcctgtga ctttgaagta
     2701 caaagtagaa taaattctgt attcatggat tggatgctga gcattcttac tcattaattt
     2761 aggtggctag gaattaatga aaaaattttc aagttaaaat acaggttata acagtataat
     2821 attcttttct aagttttcct ttcagattta ctgttatgtt ggctttagtg taagacaagg
     2881 tcactgtgct gattgatttt tatctaaaaa aattcatttt tatatcgaag aaaaaatatt
     2941 ttattcaaac attagatttg aaagagtaat agtttataaa ataaggaagt aacatgtgag
     3001 gctctcccct cctcccctcc cccatcataa agatattttg aaatcccaag gtgccctcat
     3061 acctcttgac attaatgact tcatttttta gcttgaattt gttagcaaat attaatggca
     3121 gaaaaccaaa aactaaattt atggtatcga gagcaccacc ttctcttttt gcccatattg
     3181 cactttgatt tcaaactatg cactgtgaag agattttttt ccccaggtag gaagggatgt
     3241 tccctgttat cgttatttta ctgagctgtg gaaactcatt tttacccttt ttaaagtcat
     3301 aaatatggac ttaatagccc ttaaccttca agtccaagca gttccaatca ggctgactta
     3361 ggatcatgct ggaaggaagc caagtctcaa gaactgtcag caacagaaaa tgagatatga
     3421 tgctagagag catttgtcct tgactgtaag tacagaggct taggggaagt gagacataag
     3481 cttgtaggaa taataaacgt tctacaataa acgttttggc tatcttaagc cattgtatgg
     3541 gggggattag gaagtaggag ggattgtcct acaacaggcc cttatacttc aacagcccaa
     3601 atattttggt ttattccttt ttatccataa agtctttatt cttcaagttt gggtattata
     3661 gattaccatt gttagcctcc cttaatggga ccatatgagc caggctttta agacggactc
     3721 attttgaatt gagtttcaaa ctttgaaagt gcaaggattt aatttttaag ttgaatccca
     3781 tgaaaggtgt catcatcact aggaataaca ccctttgtaa ctacatatag aactgttaca
     3841 atggtagcag aaaatcctat tgatttcatc tcacccattt cagatacctt cagtgaaatt
     3901 aagggtagtg tgatgtccca tgtaataaaa caggcttaga acatcaattc ctctgctgcc
     3961 atttatgtgc ttttattccc ccttcaaaaa ttttttattg gatatatata tttttcagta
     4021 ttcagactct gaaatctgtc attacaatca agtgcttgtc acagctgaaa gatttctggt
     4081 ggcttaaagt ttactagcag taaatttatg atatggtaca gttctttgta cagaaaggca
     4141 ccattatgtt agaataaatt tccagaactt agtgggaaga atcaggtttt aaccttgtgt
     4201 gtctgttact tctgagtgca tttctataat ataaatgttg atttgcgtca acattataaa
     4261 agagcacttc tgtggctcat ttaaaagaaa aaaaaggtta agtcttttaa attttggcct
     4321 tgtttccaaa tcctgtcttc catgttgttt ttctgcactt cctgaccact cttataaaaa
     4381 gctcacaaac ctttgaagaa actggagtag gctgttattt cattaaggtt tacaaattaa
     4441 atgcttgatt tttaatgtat tctctactgt tttgattttt ggattttctt aattcaacat
     4501 ttgaagatgt gccttttggg taatgttaat cacgaatgta gtaatatttc atgattgtaa
     4561 taatgttaca tgatcatggg tgtggcattc tctaacaaat gatgtgaaag gaaagtagtt
     4621 ttcataaaac tcatggctct gattgcacag tcccacttgc ttttgatatt gctatgtcaa
     4681 agcaaagggc acttctttta aggctacttt cagaactttg tattttcgct aaactataaa
     4741 tgcagttgtg tccagttgga ggccagggta ccacatgtca caggcggcct tacatgtgtg
     4801 ccgtgaaaag gttcaaagac cgttaacaaa aaagtaagtc tggccgggca tggtggctca
     4861 agcctgtaat cccagcactt tgggaggccg aggcgggcgg atcacttgag accaggagtt
     4921 cgagaccagc ctggccaaca tggtgtgaga ccccgtctct actaaaaata aaaaaattag
     4981 ccgggtgcgg tggcacacac ctgtaatccc agctaccggg gaggctgagg caggagaatc
     5041 gctagaacct gggaggcgga ggttgcagtc agccaagata gcaccactgc actccaggct
     5101 gggtgacaga gcgagactcc atgtcaaaaa aaaaagaatg tgggaactca aattttcatt
     5161 tttaatgtaa tccccaagat tttctccaag aaataaattg ttgttttgta ttttgtaact
     5221 taaaagcagc tattggtaaa tttctgagat atagcaggag accaaaacat gtttggaaag
     5281 agaataaata tatgaagaga gactggttgt tttattttca atgtattgaa tatattagac
     5341 tactatttat tttctgagtg gatgtgatta aatattcaga cgtttagtgt atgattaaga
     5401 gtgagataga gatttctttg gagtaattaa atccatcctg tcttttacac aatggacaaa
     5461 tcaagttaaa aaaattttta gatagcttag cttgttttct ggtgctgttg acctgcaaga
     5521 gaatatactt ttgcatatac aggtgaaagt tttgcaattt atgtatacaa gaaattgtag
     5581 gcattaaaaa tattttattg a
//
