LOCUS       NM_018327               6191 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens serine palmitoyltransferase long chain base subunit 3
            (SPTLC3), transcript variant 1, mRNA.
ACCESSION   NM_018327
VERSION     NM_018327.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Lone,M.A., Bourquin,F. and Hornemann,T.
  TITLE     Serine Palmitoyltransferase Subunit 3 and Metabolic Diseases
  JOURNAL   Adv Exp Med Biol 1372, 47-56 (2022)
   PUBMED   35503173
  REMARK    GeneRIF: Serine Palmitoyltransferase Subunit 3 and Metabolic
            Diseases.
            Review article
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Luo,C., Liu,H., Wang,X., Xia,L., Huang,H., Peng,X., Xia,C. and
            Liu,L.
  TITLE     The associations between individual plasma SFAs, serine
            palmitoyl-transferase long-chain base subunit 3 gene rs680379
            polymorphism, and type 2 diabetes among Chinese adults
  JOURNAL   Am J Clin Nutr 114 (2), 704-712 (2021)
   PUBMED   33964854
  REMARK    GeneRIF: The associations between individual plasma SFAs, serine
            palmitoyl-transferase long-chain base subunit 3 gene rs680379
            polymorphism, and type 2 diabetes among Chinese adults.
REFERENCE   3  (bases 1 to 6191)
  AUTHORS   Wang,Y., Niu,Y., Zhang,Z., Gable,K., Gupta,S.D., Somashekarappa,N.,
            Han,G., Zhao,H., Myasnikov,A.G., Kalathur,R.C., Dunn,T.M. and
            Lee,C.H.
  TITLE     Structural insights into the regulation of human serine
            palmitoyltransferase complexes
  JOURNAL   Nat Struct Mol Biol 28 (3), 240-248 (2021)
   PUBMED   33558761
REFERENCE   4  (bases 1 to 6191)
  AUTHORS   Nel,M., Jalali Sefid Dashti,M., Gamieldien,J. and Heckmann,J.M.
  TITLE     Exome sequencing identifies targets in the treatment-resistant
            ophthalmoplegic subphenotype of myasthenia gravis
  JOURNAL   Neuromuscul Disord 27 (9), 816-825 (2017)
   PUBMED   28673556
  REMARK    GeneRIF: This study demonstrated that the association of
            SPTLC3insACAC (p = 0.055) with the treatment-resistant
            ophthalmoplegic subphenotype of myasthenia gravis.
REFERENCE   5  (bases 1 to 6191)
  AUTHORS   Zhang,Q.H., Yin,R.X., Gao,H., Huang,F., Wu,J.Z., Pan,S.L., Lin,W.X.
            and Yang,D.Z.
  TITLE     Association of the SPTLC3 rs364585 polymorphism and serum lipid
            profiles in two Chinese ethnic groups
  JOURNAL   Lipids Health Dis 16 (1), 1 (2017)
   PUBMED   28056980
  REMARK    GeneRIF: The association between the SPTLC3 rs364585 SNP and serum
            total cholesterol, high-density lipoprotein cholesterol and
            low-density lipoprotein cholesterol levels in the Han and Jing
            populations
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6191)
  AUTHORS   Hornemann,T., Penno,A., Rutti,M.F., Ernst,D., Kivrak-Pfiffner,F.,
            Rohrer,L. and von Eckardstein,A.
  TITLE     The SPTLC3 subunit of serine palmitoyltransferase generates short
            chain sphingoid bases
  JOURNAL   J Biol Chem 284 (39), 26322-26330 (2009)
   PUBMED   19648650
  REMARK    GeneRIF: Data show that the SPTLC3 subunit generates
            C(16)-sphingoid bases and that sphingolipids with a C(16) backbone
            constitute a significant proportion of plasma sphingolipids.
REFERENCE   7  (bases 1 to 6191)
  AUTHORS   Han,G., Gupta,S.D., Gable,K., Niranjanakumari,S., Moitra,P.,
            Eichler,F., Brown,R.H. Jr., Harmon,J.M. and Dunn,T.M.
  TITLE     Identification of small subunits of mammalian serine
            palmitoyltransferase that confer distinct acyl-CoA substrate
            specificities
  JOURNAL   Proc Natl Acad Sci U S A 106 (20), 8186-8191 (2009)
   PUBMED   19416851
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2009 Jun 16;106(24):9931]
REFERENCE   8  (bases 1 to 6191)
  AUTHORS   Hornemann,T., Wei,Y. and von Eckardstein,A.
  TITLE     Is the mammalian serine palmitoyltransferase a high-molecular-mass
            complex?
  JOURNAL   Biochem J 405 (1), 157-164 (2007)
   PUBMED   17331073
  REMARK    GeneRIF: Results suggest that functional serine
            palmitoyltransferase is not a dimer, but a higher organized
            complex, composed of three distinct subunits (SPTLC1, SPTLC2 and
            SPTLC3) with a molecular mass of 480 kDa.
REFERENCE   9  (bases 1 to 6191)
  AUTHORS   Hornemann,T., Richard,S., Rutti,M.F., Wei,Y. and von Eckardstein,A.
  TITLE     Cloning and initial characterization of a new subunit for mammalian
            serine-palmitoyltransferase
  JOURNAL   J Biol Chem 281 (49), 37275-37281 (2006)
   PUBMED   17023427
  REMARK    GeneRIF: cloning and expression in tissues and cell lines
REFERENCE   10 (bases 1 to 6191)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL133331.27, AL445589.6,
            AL109983.2 and AL050320.19.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_018327.3.
            
            Summary: This gene encodes a subunit of the serine
            palmitoyltransferase complex which catalyzes the rate-limiting step
            in sphingolipid biosynthesis. This subunit metabolizes lauroyl- and
            myristoyl-CoA and generates C14 and C16-sphingoid bases. [provided
            by RefSeq, Mar 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.20299.1,
                                           SRR1803613.142798.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399002.7/ ENSP00000381968.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-413               AL133331.27        71361-71773
            414-599             AL445589.6         26324-26509
            600-754             AL109983.2         13443-13597
            755-903             AL109983.2         15536-15684
            904-1028            AL109983.2         32270-32394
            1029-1122           AL109983.2         34670-34763
            1123-1228           AL109983.2         51298-51403
            1229-1448           AL109983.2         58692-58911
            1449-1575           AL109983.2         67777-67903
            1576-1711           AL109983.2         95189-95324
            1712-1841           AL109983.2         101189-101318
            1842-6191           AL050320.19        1127-5476
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.1"
     gene            1..6191
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /note="serine palmitoyltransferase long chain base subunit
                     3"
                     /db_xref="GeneID:55304"
                     /db_xref="HGNC:HGNC:16253"
                     /db_xref="MIM:611120"
     exon            1..413
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     CDS             297..1955
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /EC_number="2.3.1.50"
                     /note="serine palmitoyltransferase, long chain base
                     subunit 2-like (aminotransferase 2); long chain base
                     biosynthesis protein 3; serine-palmitoyl-CoA transferase
                     3; long chain base biosynthesis protein 2b"
                     /codon_start=1
                     /product="serine palmitoyltransferase 3"
                     /protein_id="NP_060797.2"
                     /db_xref="CCDS:CCDS13115.2"
                     /db_xref="GeneID:55304"
                     /db_xref="HGNC:HGNC:16253"
                     /db_xref="MIM:611120"
                     /translation="MANPGGGAVCNGKLHNHKKQSNGSQSRNCTKNGIVKEAQQNGKP
                     HFYDKLIVESFEEAPLHVMVFTYMGYGIGTLFGYLRDFLRNWGIEKCNAAVERKEQKD
                     FVPLYQDFENFYTRNLYMRIRDNWNRPICSAPGPLFDLMERVSDDYNWTFRFTGRVIK
                     DVINMGSYNFLGLAAKYDESMRTIKDVLEVYGTGVASTRHEMGTLDKHKELEDLVAKF
                     LNVEAAMVFGMGFATNSMNIPALVGKGCLILSDELNHTSLVLGARLSGATIRIFKHNN
                     TQSLEKLLRDAVIYGQPRTRRAWKKILILVEGVYSMEGSIVHLPQIIALKKKYKAYLY
                     IDEAHSIGAVGPTGRGVTEFFGLDPHEVDVLMGTFTKSFGASGGYIAGRKDLVDYLRV
                     HSHSAVYASSMSPPIAEQIIRSLKLIMGLDGTTQGLQRVQQLAKNTRYFRQRLQEMGF
                     IIYGNENASVVPLLLYMPGKVAAFARHMLEKKIGVVVVGFPATPLAEARARFCVSAAH
                     TREMLDTVLEALDEMGDLLQLKYSRHKKSARPELYDETSFELED"
     exon            414..599
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            600..754
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            755..903
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1028
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1122
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1228
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1448
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1575
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1711
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1841
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1842..6191
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3660..3665
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /note="hexamer: AATAAA"
     polyA_site      3685
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /note="major polyA site"
     regulatory      3829..3834
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /note="hexamer: AATAAA"
     polyA_site      3854
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /note="major polyA site"
     regulatory      6170..6175
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
                     /note="hexamer: AATAAA"
     polyA_site      6191
                     /gene="SPTLC3"
                     /gene_synonym="C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB
                     3; LCB2B; LCB3; SPT 3; SPT3; SPTLC2L"
ORIGIN      
        1 agtccccaga aggagccagc atggacaatc tcctttacag tttcggaagc aggtttgttg
       61 ccatggagtt cacattttga cgggagttga gaagtataaa ggtaaccatt tgttttagtt
      121 tcaacgatct gacaaaaaga taggctgttg ctcttcttct ggaaaagcct gattggtaag
      181 attcctttaa gggctcagcc ccaaagagct ttatcccatc ccctcgcaga ctgaaaacta
      241 aagcctgcag agacctctga aggaaaacct gtcccgggct ctgtcacttc acacccatgg
      301 ctaaccctgg aggtggtgct gtttgcaacg ggaaacttca caatcacaag aaacagagca
      361 atggctcaca aagcagaaac tgcacaaaga atggaatagt gaaggaagcc cagcaaaatg
      421 ggaagccaca tttttatgat aagctcattg ttgaatcgtt tgaggaagca ccccttcatg
      481 ttatggtttt cacttacatg ggatatggaa ttggaaccct gtttggctat ctcagagact
      541 ttttaagaaa ctggggaata gaaaaatgca acgcagctgt ggaaagaaaa gaacaaaaag
      601 attttgtgcc actgtatcaa gactttgaaa atttttatac aagaaacctt tacatgcgaa
      661 tcagagacaa ctggaaccgg cccatctgca gtgccccagg gcctctgttt gatttgatgg
      721 agagggtatc agacgactat aactggacgt ttaggtttac tggaagagtc atcaaagatg
      781 tcatcaacat gggctcctat aacttccttg gtcttgcagc caagtatgat gagtctatga
      841 ggacaataaa ggatgtttta gaggtgtatg gcacaggcgt ggccagcacc aggcatgaaa
      901 tgggcacctt ggataagcac aaggagttgg aggaccttgt ggctaagttc ctgaatgtgg
      961 aagcagctat ggtctttggg atgggattcg caactaactc aatgaatatc ccagcattag
     1021 ttggaaaggg atgcctcatt ttaagtgatg agttaaacca cacatcgctt gtgcttgggg
     1081 cccgactctc aggtgcaacc ataagaatct tcaaacacaa caacacacaa agcctagaga
     1141 agctcctgag agatgctgtc atctatggcc agcctcgaac ccgcagagct tggaaaaaga
     1201 ttctcatcct ggtggagggt gtctacagca tggaaggttc catcgtgcat ctgccccaga
     1261 tcatagctct aaagaagaaa tacaaggctt acctctacat agatgaagct cacagtattg
     1321 gggccgtggg cccaaccggc cggggtgtca cggagttctt tggactagac cctcatgaag
     1381 ttgatgtgct catgggcaca ttcaccaaaa gttttggagc ttcaggaggt tacatagctg
     1441 gaaggaagga cctcgtggat tatttacggg ttcactcgca tagtgctgtt tatgcttcat
     1501 ccatgagccc accgatagca gagcaaatca tcagatcact aaaacttatc atgggactgg
     1561 atgggaccac tcaagggctg cagagagtac agcaacttgc gaaaaacaca agatacttca
     1621 gacaaagact gcaggaaatg ggattcatta tctatggcaa tgagaatgct tctgttgttc
     1681 ctctgcttct ttatatgcct ggtaaagtag cggcttttgc aaggcatatg ctagagaaaa
     1741 aaattggagt ggtggtcgtg ggatttccag ccactcccct cgcagaagct cgggctcggt
     1801 tttgtgtttc agcggcacat acccgggaga tgttagacac ggttttagaa gctcttgatg
     1861 aaatgggtga tctcttgcaa ctgaaatatt cccggcacaa gaagtcagca cgtcctgagc
     1921 tctatgatga gacgagcttt gaactcgaag attaagtttc ctggtcctga atgacacata
     1981 aagactttgc gagaaagacc tccctccttg cctcacaagg aatataaatg gatttctccc
     2041 ccttcctcag gacaattttg gttcccagac cagcttgatt gaactgaggg agacgttgtt
     2101 gtttttaatg tctccagctt ggactgcaga gacaaaaaca tgattccaga tttaagtctc
     2161 tcttcttcca agtattctac tagaaataca cacacacaca cacacacact tctgagaata
     2221 tttttaatgg caataagcct gtgttttagc tgctactgtg cagacccttt cagggattcc
     2281 aaccaaatgc aaatgagaga atttaaaatt tttattcagt aagttcacta tgtgtttact
     2341 tattccatct gcagatgtaa gtgagtgtgg tggacagtag ccaccttcct tgttccactc
     2401 ataaaagcat cagctagatc tcatctgtat catgggaagt tccaggcagg agggtaaaga
     2461 aatgttgttt ctaccatttg tcacatttgg acgtttttca cagacaagtg tcaaaagaca
     2521 tagttaatgt ttcgaggggg aaagcagaac tgatcaactg cgactagaga cgtctttgaa
     2581 ggaaattttc cttttcctct tgctggtctc ctacagtttt acagctgagc ttttgaggtt
     2641 tggaaaattc aagatgtttg tttcataaga aaaggggcag aaagcaagca caagacactt
     2701 ttaggtctag actaaaatgg tagttacaac aatttgaacc ttgttggtgc agtgcacggt
     2761 gaatgcttac cctgcacagc ctctattacc ttaaggaatt agttgtcatt ttcctattga
     2821 attttgagta aatggtgaaa ttcagtgtcc tttagaatcg actcccaaga ctatatttga
     2881 agaatgcatt gattcaagaa ggacatttaa aagcaaattc tgactttttc aagaccaaca
     2941 cacttgttta ggcctattaa attgtactat cacttgttac atgccctctg aattgggaga
     3001 aagtgggctt gcacactttg aggtaactaa ctgtaattta cttgtgttct ctctttcttg
     3061 ctctccttct caacatgaac acaaacctct atggaaaagt agcctcttgt taatctgatc
     3121 tagtttgtat ggaaaaagcc catggagaac cttatcttta acaagctccc agagggtgcc
     3181 cagatttgga aattctagag agcagtggtg accttttagc aaagcttctg taacagttcg
     3241 aatgagttgc agaacattcc actccatcaa atgacactgt aaacaaatca cttcaagaga
     3301 ggcttggttt tgtgtggcac agatggaccc aagctttcat gctgtgcact gagatagaaa
     3361 ctccacccgc agcgcctgcg atggatggag cagtgtgccc tgatgctcaa agcgtattaa
     3421 aggaaaaaaa gtgatcagca tggaaagttt ttatggggaa attataactc caagtgggtg
     3481 cattggttta aaaatggatc acaatgatag agttcttcat gaatgtttac aagttgtaag
     3541 gaataccgtt agtgaaagag gaaaagaagt gggttctgaa aatgcagttt cccatccaat
     3601 gattttgata acaaaattcc aacttttctc aatgaaactg atgcagtatt atttgtgcaa
     3661 ataaaatgtg tcataaatat gcaaagaaag ggagacatac tgttcttatc ttgaatatgt
     3721 gcatttaaat gaattgtcca aaatgcataa atgccttgcg ttctataaag gaaacaggag
     3781 gtcaaaaaga cagcgagata tctagtcctt ccacaaggta tggaaagcaa taaacatctt
     3841 cctttctttc tggatctgtc tgtgatgttt acacacacct tcacatctac acttcaagtt
     3901 ttaagcccct agacatgtgt cttcatgagc aggaatgtcc actattatta ctttcatgga
     3961 ggatattggg tctaattcat tgtgggaaac tacttaccta cccagcatgg aaggcaggga
     4021 ggaagttagt ctcttttcat caagattcaa gagtacaggg gcaatttatt acactgcaat
     4081 gtacaaaaca aaaggacctg tttctgttag agagtccatg ttgaatatac aggtatgaga
     4141 acaactgttg atatacaggt ataatccaag tagaatgctt ccacagtgca ttagaaggtg
     4201 cccaagctcc acagggccaa taatattaga gtatatgtca tatagaataa ggttaattag
     4261 gagggtgatt gtgaataatg agatttttaa tttttttaag aaaaagctct caaatgctag
     4321 gctaataagt tcatgatttt aattcatgat tttaattcat gattaaaatt atttgattat
     4381 tgagtcttga taattatatc atcaaccatt accctctaca tcaccgcccc gaccttagct
     4441 ctaattatat cagagccaaa aatgtgtctt tggttagatc tgttggaaaa gagaattttc
     4501 tataatgtta tgcgcactca gcccttctgt tcacatttac acacatgcac acacacatac
     4561 acatatgcat ttttccttta cggagtaagc aactatgaaa atctggttgg ttgagaaata
     4621 aatggaagat gttcttgaaa atgacctcag gaaagcagaa gataaaggtt ccagatgata
     4681 tttgtaagga ggctatctct gtataataag gaaatggcat agacttattc ctaatctcat
     4741 tatgaaacga tggtgtcatg gacgtttaac catattgcct ttcagcatgc tttgatttta
     4801 cagagacgac aggctttgga atttactagg aagctggcta aaatccatac ccctaggccc
     4861 tacaccagac ctttactgaa acataatagt tgtagtgccc aggaatctac atttttaacc
     4921 agccttcctg cgtgattcta atctatgcca aaatttaaga acattattca aactctaatt
     4981 tgataaacca attgactcta atagcaggaa ccttgttctg attctcttgt tcataattaa
     5041 gtgtatatat actggtaaaa ttaatttatt ccacagtcaa ccttctcttc tcattgggac
     5101 attttgttcc cattttccaa gtaacttctc tttctacaaa aattgatcca atattgaaga
     5161 accatctgcg attcatttgg gtaaaattat gccttcttca gttttctgta tcattcccta
     5221 taagatgccc atttgctttg aatgtggtat ctttcttttc tttctttctt tttttttttt
     5281 tttttgagac agagttttgt tcttcttgtt gcccaggcta tagtgcaatg gcaagatctc
     5341 ggctcattgc aacctccacc tcccgggttc aagcgattct cctgcctcag cctcctgagg
     5401 agctgagttt acaggcatgt gccaccaagc atggctaatt ttctattttt agtagagaca
     5461 aggtttttcc ctgttggtca ggctggtctc gaactcctga ccttaggtga tcctcccacc
     5521 tcggcctccc aaagtgctgg gattacaggc gtgagccacc gtgcccggcc tgaatgtagt
     5581 atctttcttc tgttttcttt tcctgctctt taaaaaacaa ccttcaacct ctgattattt
     5641 attccaacaa cagctcttca gtagggtaaa agtcttaaca gttcttcaaa cgaacaatat
     5701 tcttttgtat ttttcttcag agatagcaaa tgttataaat ggcctactct gatttaaaaa
     5761 cctaagttcc gatgatgatg aaacattaac tcttaaatat acattttgat ttatagcaat
     5821 aataacaatt gtaacaacag caattaagaa cagcatctgg tgaattcttg gcaatgtttc
     5881 ctggtctcaa aacagtaaga acaatttgaa gtctgttctt tagaatgccc tactcagctg
     5941 cataatgttt agaaaattaa agagtctgta ttttaaatat ttttaggtaa ttattgcggg
     6001 acattgtctt atggtgtcct gatgtacgtg gggtgtgcat gtgtgtgtgt gtgtgtgtgc
     6061 atgtgtcttt gtgtgtgtga gaaggacaga gagaaatgta tgtttcactg aaacatttga
     6121 aacgaccaat ttcactttat tgtgcctgta aaatgttagt tttaaaataa ataaaaataa
     6181 tatgaaaata a
//
