LOCUS       NM_004863               8034 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens serine palmitoyltransferase long chain base subunit 2
            (SPTLC2), mRNA.
ACCESSION   NM_004863
VERSION     NM_004863.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Naruse,H., Ishiura,H., Esaki,K., Mitsui,J., Satake,W., Greimel,P.,
            Shingai,N., Machino,Y., Kokubo,Y., Hamaguchi,H., Oda,T., Ikkaku,T.,
            Yokota,I., Takahashi,Y., Suzuki,Y., Matsukawa,T., Goto,J., Koh,K.,
            Takiyama,Y., Morishita,S., Yoshikawa,T., Tsuji,S. and Toda,T.
  TITLE     SPTLC2 variants are associated with early-onset ALS and FTD due to
            aberrant sphingolipid synthesis
  JOURNAL   Ann Clin Transl Neurol 11 (4), 946-957 (2024)
   PUBMED   38316966
  REMARK    GeneRIF: SPTLC2 variants are associated with early-onset ALS and
            FTD due to aberrant sphingolipid synthesis.
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   Dohrn,M.F., Beijer,D., Lone,M.A., Bayraktar,E., Oflazer,P.,
            Orbach,R., Donkervoort,S., Foley,A.R., Rose,A., Lyons,M.,
            Louie,R.J., Gable,K., Dunn,T., Chen,S., Danzi,M.C., Synofzik,M.,
            Bonnemann,C.G., Nazli Basak,A., Hornemann,T. and Zuchner,S.
  TITLE     Recurrent de-novo gain-of-function mutation in SPTLC2 confirms
            dysregulated sphingolipid production to cause juvenile amyotrophic
            lateral sclerosis
  JOURNAL   J Neurol Neurosurg Psychiatry 95 (3), 201-205 (2024)
   PUBMED   38041684
  REMARK    GeneRIF: Recurrent de-novo gain-of-function mutation in SPTLC2
            confirms dysregulated sphingolipid production to cause juvenile
            amyotrophic lateral sclerosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8034)
  AUTHORS   Syeda,S.B., Lone,M.A., Mohassel,P., Donkervoort,S., Munot,P.,
            Franca,M.C. Jr., Galarza-Brito,J.E., Eckenweiler,M., Asamoah,A.,
            Gable,K., Majumdar,A., Schumann,A., Gupta,S.D., Lakhotia,A.,
            Shieh,P.B., Foley,A.R., Jackson,K.E., Chao,K.R., Winder,T.L.,
            Catapano,F., Feng,L., Kirschner,J., Muntoni,F., Dunn,T.M.,
            Hornemann,T. and Bonnemann,C.G.
  TITLE     Recurrent de novo SPTLC2 variant causes childhood-onset amyotrophic
            lateral sclerosis (ALS) by excess sphingolipid synthesis
  JOURNAL   J Neurol Neurosurg Psychiatry 95 (2), 103-113 (2024)
   PUBMED   38041679
  REMARK    GeneRIF: Recurrent de novo SPTLC2 variant causes childhood-onset
            amyotrophic lateral sclerosis (ALS) by excess sphingolipid
            synthesis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8034)
  AUTHORS   Wilson,L.M.Q., Saba,S., Li,J., Prasov,L. and Miller,J.M.L.
  TITLE     Specific Deoxyceramide Species Correlate with Expression of Macular
            Telangiectasia Type 2 (MacTel2) in a SPTLC2 Carrier HSAN1 Family
  JOURNAL   Genes (Basel) 14 (4), 931 (2023)
   PUBMED   37107689
  REMARK    GeneRIF: Specific Deoxyceramide Species Correlate with Expression
            of Macular Telangiectasia Type 2 (MacTel2) in a SPTLC2 Carrier
            HSAN1 Family.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8034)
  AUTHORS   Li,C., Hou,Y., Wei,Q., Lin,J., Jiang,Z., Jiang,Q., Yang,T.,
            Xiao,Y., Huang,J., Cheng,Y., Ou,R., Liu,K., Chen,X., Song,W.,
            Zhao,B., Wu,Y., Cao,B., Chen,Y. and Shang,H.
  TITLE     Mutation screening of SPTLC1 and SPTLC2 in amyotrophic lateral
            sclerosis
  JOURNAL   Hum Genomics 17 (1), 28 (2023)
   PUBMED   36966328
  REMARK    GeneRIF: Mutation screening of SPTLC1 and SPTLC2 in amyotrophic
            lateral sclerosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8034)
  AUTHORS   Dawkins,J.L., Brahmbhatt,S., Auer-Grumbach,M., Wagner,K.,
            Hartung,H.P., Verhoeven,K., Timmerman,V., De Jonghe,P.,
            Kennerson,M., LeGuern,E. and Nicholson,G.A.
  TITLE     Exclusion of serine palmitoyltransferase long chain base subunit 2
            (SPTLC2) as a common cause for hereditary sensory neuropathy
  JOURNAL   Neuromuscul Disord 12 (7-8), 656-658 (2002)
   PUBMED   12207934
  REMARK    GeneRIF: results suggest that SPTLC2 mutations are not a common
            cause for genetic sensory neuropathies.
REFERENCE   7  (bases 1 to 8034)
  AUTHORS   Hanada,K., Hara,T. and Nishijima,M.
  TITLE     Purification of the serine palmitoyltransferase complex responsible
            for sphingoid base synthesis by using affinity peptide
            chromatography techniques
  JOURNAL   J Biol Chem 275 (12), 8409-8415 (2000)
   PUBMED   10722674
REFERENCE   8  (bases 1 to 8034)
  AUTHORS   Weiss,B. and Stoffel,W.
  TITLE     Human and murine serine-palmitoyl-CoA transferase--cloning,
            expression and characterization of the key enzyme in sphingolipid
            synthesis
  JOURNAL   Eur J Biochem 249 (1), 239-247 (1997)
   PUBMED   9363775
REFERENCE   9  (bases 1 to 8034)
  AUTHORS   Nagiec,M.M., Lester,R.L. and Dickson,R.C.
  TITLE     Sphingolipid synthesis: identification and characterization of
            mammalian cDNAs encoding the Lcb2 subunit of serine
            palmitoyltransferase
  JOURNAL   Gene 177 (1-2), 237-241 (1996)
   PUBMED   8921873
REFERENCE   10 (bases 1 to 8034)
  AUTHORS   Takeda,J., Yano,H., Eng,S., Zeng,Y. and Bell,G.I.
  TITLE     A molecular inventory of human pancreatic islets: sequence analysis
            of 1000 cDNA clones
  JOURNAL   Hum Mol Genet 2 (11), 1793-1798 (1993)
   PUBMED   7506601
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB011098.1, BQ338973.1 and
            AF111168.2.
            
            On Feb 27, 2020 this sequence version replaced NM_004863.3.
            
            Summary: This gene encodes a long chain base subunit of serine
            palmitoyltransferase. Serine palmitoyltransferase, which consists
            of two different subunits, is the key enzyme in sphingolipid
            biosynthesis. It catalyzes the
            pyridoxal-5-prime-phosphate-dependent condensation of L-serine and
            palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were
            identified in patients with hereditary sensory neuropathy type I.
            [provided by RefSeq, Mar 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB011098.1, SRR14038191.1426809.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000216484.7/ ENSP00000216484.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-843               AB011098.1         131-973
            844-978             BQ338973.1         306-440
            979-3550            AB011098.1         1109-3680
            3551-8034           AF111168.2         108592-113075       c
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.3"
     gene            1..8034
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /note="serine palmitoyltransferase long chain base subunit
                     2"
                     /db_xref="GeneID:9517"
                     /db_xref="HGNC:HGNC:11278"
                     /db_xref="MIM:605713"
     exon            1..190
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..1747
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /EC_number="2.3.1.50"
                     /note="serine palmitoyltransferase, subunit II; LCB 2; SPT
                     2; serine-palmitoyl-CoA transferase 2; long chain base
                     biosynthesis protein 2a"
                     /codon_start=1
                     /product="serine palmitoyltransferase 2"
                     /protein_id="NP_004854.1"
                     /db_xref="CCDS:CCDS9865.1"
                     /db_xref="GeneID:9517"
                     /db_xref="HGNC:HGNC:11278"
                     /db_xref="MIM:605713"
                     /translation="MRPEPGGCCCRRTVRANGCVANGEVRNGYVRSSAAAAAAAAAGQ
                     IHHVTQNGGLYKRPFNEAFEETPMLVAVLTYVGYGVLTLFGYLRDFLRYWRIEKCHHA
                     TEREEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICSVPGARVDIMERQSHDYNWSF
                     KYTGNIIKGVINMGSYNYLGFARNTGSCQEAAAKVLEEYGAGVCSTRQEIGNLDKHEE
                     LEELVARFLGVEAAMAYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGAT
                     IRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALK
                     KKYKAYLYLDEAHSIGALGPTGRGVVEYFGLDPEDVDVMMGTFTKSFGASGGYIGGKK
                     ELIDYLRTHSHSAVYATSLSPPVVEQIITSMKCIMGQDGTSLGKECVQQLAENTRYFR
                     RRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRA
                     RFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED"
     exon            191..385
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     exon            386..540
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     exon            541..689
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     exon            690..814
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     exon            815..908
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1014
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1234
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1361
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1497
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1627
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..8034
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1990..1995
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /note="hexamer: ATTAAA"
     polyA_site      2014
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /note="major polyA site"
     regulatory      8014..8019
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /note="hexamer: AATAAA"
     polyA_site      8034
                     /gene="SPTLC2"
                     /gene_synonym="hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2"
                     /note="major polyA site"
ORIGIN      
        1 agccgccgcc gctgccaccg cctacagagc ctgccttgcg cctggtgctg ccaggaagat
       61 gcggccggag cccggaggct gctgctgccg ccgcacggtg cgggcgaatg gctgcgtggc
      121 gaacggggaa gtacggaacg ggtacgtgag gagcagcgct gcagccgcag ccgcagccgc
      181 cgccggccag atccatcatg ttacacaaaa tggaggacta tataaaagac cgtttaatga
      241 agcttttgaa gaaacaccaa tgctggttgc tgtgctcacg tatgtggggt atggcgtact
      301 caccctcttt ggatatcttc gagatttctt gaggtattgg agaattgaaa agtgtcacca
      361 tgcaacagaa agagaagaac aaaaggactt tgtgtcattg tatcaagatt ttgaaaactt
      421 ttatacaagg aatctgtaca tgaggataag agacaactgg aatcggccaa tctgtagtgt
      481 gcctggagcc agggtggaca tcatggagag acagtctcat gattataact ggtccttcaa
      541 gtatacaggg aatataataa agggtgttat aaacatgggt tcctacaact atcttggatt
      601 tgcacggaat actggatcat gtcaagaagc agccgccaaa gtccttgagg agtatggagc
      661 tggagtgtgc agtactcggc aggaaattgg aaacctggac aagcatgaag aactagagga
      721 gcttgtagca aggttcttag gagtagaagc tgctatggcg tatggcatgg gatttgcaac
      781 gaattcaatg aacattcctg ctcttgttgg caaaggttgc ctgattctga gtgatgaact
      841 gaatcatgca tcactggttc tgggagccag actgtcagga gcaaccatta gaatcttcaa
      901 acacaacaat atgcaaagcc tagagaagct attgaaagat gccattgttt atggtcagcc
      961 tcggacacga aggccctgga agaaaattct catccttgtg gaaggaatat atagcatgga
     1021 gggatctatt gttcgtcttc ctgaagtgat tgccctcaag aagaaataca aggcatactt
     1081 gtatctggat gaggctcaca gcattggcgc cctgggcccc acaggccggg gtgtggtgga
     1141 gtactttggc ctggatcccg aggatgtgga tgttatgatg ggaacgttca caaagagttt
     1201 tggtgcttct ggaggatata ttggaggcaa gaaggagctg atagactacc tgcgaacaca
     1261 ttctcatagt gcagtgtatg ccacgtcatt gtcacctcct gtagtggagc agatcatcac
     1321 ctccatgaag tgcatcatgg ggcaggatgg caccagcctt ggtaaagagt gtgtacaaca
     1381 gttagctgaa aacaccaggt atttcaggag acgcctgaaa gagatgggct tcatcatcta
     1441 tggaaatgaa gactctccag tagtgccttt gatgctctac atgcctgcca aaattggcgc
     1501 ctttggacgg gagatgctga agcggaacat cggtgtcgtt gtggttggat ttcctgccac
     1561 cccaattatt gagtccagag ccaggttttg cctgtcagca gctcatacca aagaaatact
     1621 tgatactgct ttaaaggaga tagatgaagt tggggaccta ttgcagctga agtattcccg
     1681 tcatcggttg gtacctctac tggacaggcc ctttgacgag acgacgtatg aagaaacaga
     1741 agactgagcc tttttggtgc tccctcagag gaactctccc tcacccagga cagcctgtgg
     1801 cctttgtgag ccagttccag gaaccacact tctgtggcca tctcacgtga aagacattgc
     1861 ctcagctact gaaggtggcc acctccactc taaatgacat tttgtaaata gtaaaaaact
     1921 gcttctaatc cttcctttgc taaatctcac ctttaaaaac gaaggtgact cactttgctt
     1981 tttcagtcca ttaaaaaaac attttatttt gcaaccattc tacttgtgaa atcacgctga
     2041 ccctagcctg tctctggcta accacacagg ccattcccct ctcccagcac cttgcagact
     2101 tgggcccatc aagagctact gctggccctg gctccgcagc ctggatactt acctggccct
     2161 cctccctagg gagcaagtgc cttccactta cttcccatcc aggtctcaga ggtctcaagg
     2221 ccaaccttgg aatccttatt taaccattca agtaatcaac ggaagttttc accctttaat
     2281 cttaagttta gccttttaag aaaaacagta agcgatgact gctgaaaggc tcattgtgta
     2341 atctcccaag ggtttggtct tattccattt tcttctggtc accagatgat ttcttccttt
     2401 accatcaaat acttcttcat aatggtcaca gtctgaggat gtgcgcaaat tctggttctt
     2461 cccaagctct aaccgtaaca cgtcccaccc cctttttaaa gcacttactg ttttcagagc
     2521 acccatatcc caccctggtg agaaggccac tctcacatct gagtgttggg tacaaagctg
     2581 ctccgtagag tgatgtgcac tcctggtggg tgaggggcag gggcagtggc agtgtgcaaa
     2641 gaattgatta ctccttgcag agcctgtggc ttgcatttcc tactgctttc tacgtttgaa
     2701 aattatgaca gtctctggct aggtctgggt ccagattagg atttaaactg ataaaggaaa
     2761 ctgttggtaa atcctctgct cagaaagcat ttatcatgtt cctatttaag gattaggttt
     2821 attaatttag gcctcttaga agctaaccca cttaaatatt actcttctga atgctagttc
     2881 tcttttattc ttgatgtcct aagtcaattg aatctggcat ctggggctag ggtctgcctg
     2941 tctacatatt ttttattttt ttctgagaaa ttctgaacac atagatctct ttcctaaact
     3001 gacattttct attttgactg ttttcatact ataaccaggt aaagggactt ctttcagaga
     3061 gctttatact gcctgaccaa agaacaaatc tgaaaatcac cattttaaag ttattttttc
     3121 agttgaacca aagtttaagt gaagaggact tttggcatat tatacccagg atcagtttgt
     3181 ctttttgtat ccatcaagta ttacaggaga aggattggga acagaatgga aaaacagtgt
     3241 atgaaagtca tgttacaggc cgagtgcggt ggctcacacc tgtaatccta gcactttggg
     3301 aggctgaggc aggtggctca cttgaggtca ggaattcaag accagcctgg ccaacatggt
     3361 gaaaccccgt ctctactaaa aagacaaaaa attagctggg cgtggtggcg ggcacctata
     3421 atcccaccta cttggtaggc tgaggcagga gaatcgcttg aacccaggag gcggaggttg
     3481 cagtgagacg agattgtgcc actgcactct agcctgggtg acagagcaaa actgtgtctc
     3541 aaaaaaaaaa gtcatgttac acatttaagt ttttgaaatt gctcctttta tcggtaaaga
     3601 ttctcaatcc aaattctcct gggtgtgttg tcatcagctg tgatatgttt gtgcacatta
     3661 cgtatagcag aggatgtaag caatattatt gtttgtgaag ttttgttttt aatgtcttga
     3721 gtatgagtta tgtttagtca ctgtcagcat ctgagaactt taataagccc ttgagatatt
     3781 ccaaagtttt attttacttt tttaaagaac agaaaaagat gaatgaaaga accaaggaga
     3841 gatgcagaga ctatatttag catgtatagg ttaaagtaag aaggaggttg tggtaactaa
     3901 ataggagtcc tataaaatca aatacattgt caaccttttc tgcacatcta gtttcctacc
     3961 atagaatccc actggaatac cacatagctt ttgcactgca gttactattt actaatgtaa
     4021 acgtagggtt tgtaaaagtc acaaacttat aagcaatgaa cttacctgct agtcttttta
     4081 ttttggcttg catgaagtca ctgcaaattc aaatgtcagt accggcattt aaaatatatc
     4141 tatatcactt tgttggtaca aagttatttc aagataagtg taattttgtt acaagtttat
     4201 tttgaagaga caaatctcct gtgatctatg caggacctct gtactttcta aagaacaaaa
     4261 tgttatgtag acattataca tggttggttg tctcttcttg aaactgtaat gtaaatctag
     4321 ggtccagtca tatcctaggt atcatcattt atccaagtac ttggaggaat acaagtatat
     4381 ataaatacag tcattgagaa taagtcgatt tgaggcatac aagagtagtt tcttacacag
     4441 tttaacacgg cctgattcaa gactctgata ggattcaaac agataccggt taaccatgac
     4501 taccaaaact gatcatctga gtcgattgat agaggtgtga ctagtcctta gcactttttc
     4561 tcattcctct ttttattcag cattgctgtt acctatttca ggtttataag acctctttca
     4621 gcagatcaca tcagaagcca ggaaatgcat agctaggaga tgtcaaaagc ccatatgagg
     4681 agtggaccaa gcagcagtgg cggtttctcc tcgcatcttt ttttttttaa gctttaactt
     4741 agcaggggca tggactttat agcacttttt caactttttg ctttgctttg gataagaaat
     4801 ccttaccttt aaaaaaagct tctagtctcc ataaccccca aagtactgct tatttgtttg
     4861 aagaatccag ccatcgtagt gctttagtca ctatcgtaaa cattcatgat agggcaagga
     4921 ttttaaaaca ggattcttgc ttctgtagtc atcaaggtga acagaagcat cctacacaac
     4981 cactaagggc tctatgtttg tgtcatgcct cttcaaacac caaggagttg aacatgcttc
     5041 cagtgatttg tctccgtaat gccttcttcc tttatttggc ctttctttct ttctgtacct
     5101 tcaagttctt gatttttaaa attccaactc tagagaaaac caatatatgg tggtgctggg
     5161 ctttgaagat agcatatcag acgccttggt tctgtttgta cacttagcct tacatttcag
     5221 gaggaggctt ttcattaggg gcttaagcta gctcctttgg cttttaaaaa aaattttttt
     5281 tcaaatttct tcattaccta agggagcctg catctaaatt tctcaactag ttcagcctag
     5341 ctgaattttc tagtgtgtta tacactttgc ttccttctta ttggtgaaaa ccagggggat
     5401 gagtggcttc catggagaga tttcctgatt tctcagggag gaaaaaagtg atgacattta
     5461 ccactacttt tatgtttttc ccctttttcc aaattgataa ggatttctgg ttcctagtga
     5521 tccgggattg ggcaacagtg cagaactgcc agtcatgccg taggccgtga agaaagaatg
     5581 tgagtaactg ttgttttgca aggatttgta gggttatggg cagttgttgt ttgaagcatt
     5641 gctatgacct aattcccaag gtatctttcc tctcttggtg ttctaggtaa gccaatgagc
     5701 tttaatctct acttgctata accgtgtgct tagaaaaaga ggtgagagta gtggttttcc
     5761 ttcaaactgt ccacattcat gaagattatg aattgttagg acagccaggg caagatagac
     5821 cctgtctcta caaaaatttt tttctaaatt aaccgggcat ggtggtgcct gcctgtagtc
     5881 ccacctgtgt gggagaatca cttgagcctg ggaggtcaag gctgcagtga gccatgattg
     5941 cacccctgca ctccagcctg ggtgacagag tgagaccctg gctcaataag agggggaaaa
     6001 aaaattgtta ggagctgggt gcggatgcag cctgcaatcc cagctacttg agaggctgag
     6061 gccggaggat tgcttaaacc caagaatttg agcgtagcct gggcaacaca gcaagacccc
     6121 atctaagaaa aaaatgtttt ttaaatcagc ttagcccaaa ggggttgtga atggggaggt
     6181 ataaaaagca aagattattt tttggctact aagccaagaa cttacaggga tttttttttt
     6241 cagtcccaga acctacagat accctgctac ttgcttcacg tggatgctca gtgcccagca
     6301 gccatcttaa tacattaaac cagtttaaaa aataccttcc atgtggagaa aaacatgtct
     6361 ttttctcgcc tcaactttat ccacatgaaa tatgtgccca tggctgggcg cagtggctca
     6421 cctgtaatcc caacactttg ggaggctgaa gcaggcagat tgcttgaggc caggagttcg
     6481 agaacagtct ggccaacatg gcgaaacctc atctctacta aaattacaaa aattagccgg
     6541 gcatggtggc acatgcctgt aatcccagct acgtcaggag gctgaggcac aggaattgct
     6601 tgaacccaag aggcagagga tgcaatgagc caagatcaca ccactgcact ccagccttgg
     6661 cgacagaggg agactctgtc tcaaaaaaaa aaaaaaaagg tgtgcccagg cccctagcca
     6721 ttgccatgtg cccagccaga gagccaaatt agagggctgg cttccctatc acacagaata
     6781 aatgctagtg ctagccaatg atccctttgc ttttaatgta tagaaaatac tgttgttcct
     6841 tttgtcattt ccagtgacat ctgttttcta agcagctctt ttctagggag gaaaccaaag
     6901 gggctaggtt aagaccctaa tagaaatgtt ttttctaatc tctggtgagt ctggaagtgt
     6961 cacattcaca gtccaccctt gggagtggct tggtggagct ggggacaagg ttttgtttac
     7021 tacatagtgc acatgataaa tggccttaaa ctgtgattct ttctggtagg ataagttata
     7081 ataaactgac cctaaagaat gcaatggctt ttaaactgca gttactgtgt tcttaatgaa
     7141 gcaataccca aagctctgtt cttttggagc acttgagggg agcttgaatg aaaggtgcag
     7201 ataagagcag taccttgatc ttatgctttc tgagtgtcct gccttgttgc catctgcatg
     7261 gatgagtgaa tgcttctatg cacgaggaga ctcaagccaa ctcagagtct gctttttcca
     7321 acgctcttcc caggtttctt ttgcaaagct tggtcatttg gcccaggtct tcctggaaag
     7381 tggagtacat gtcactgact agggtggcgt ggtgtcttta cccttaacat taagtcttgt
     7441 tacctcagtg atgtgaagcc aatggttgga attataaaaa gcatccttgc tggttcttca
     7501 caggacactg gaacccaccc tgtcaattca gctagcatgt ccacacagtc ttgatgatcc
     7561 ctctctgtaa caggcagcta acattaagag aagggggaaa gagaagaaga gagcaatagc
     7621 ttatgggaga gctgagatct tacttcgttg acccatattt ttcccctgac caagttacct
     7681 gtaaactgga atttgcaagg ggatgctgtg atgataaccc ctttctattg ctgtaatgtt
     7741 catataacct gggaaactga gagaagggga tgtgtaaata aaagcttaaa cattttagta
     7801 atgtgttaaa atgtcactct ctcttaccct gtttcccttt tttgccagat gatgattttt
     7861 ttatttttat tttgtacttt actggatgac tgtgaagcga tgagtattgg gttggggtag
     7921 gtgtgttgat tttgagagtg catgttaaga actgaagggg aactacttga gatgacttaa
     7981 gaagcatccc atgcaaatat cttgttttgc cctaataaaa tattcagaaa gata
//
