LOCUS       NM_020971               8910 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript
            variant sigma1, mRNA.
ACCESSION   NM_020971
VERSION     NM_020971.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Nassal,D.M., Shaheen,R., Patel,N.J., Yu,J., Leahy,N., Bibidakis,D.,
            Parinandi,N.L. and Hund,T.J.
  TITLE     Spectrin-Based Regulation of Cardiac Fibroblast Cell-Cell
            Communication
  JOURNAL   Cells 12 (5), 748 (2023)
   PUBMED   36899883
  REMARK    GeneRIF: Spectrin-Based Regulation of Cardiac Fibroblast Cell-Cell
            Communication.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Buelow,M., Sussmuth,D., Smith,L.D., Aryani,O., Castiglioni,C.,
            Stenzel,W., Bertini,E., Schuelke,M. and Knierim,E.
  TITLE     Novel bi-allelic variants expand the SPTBN4-related genetic and
            phenotypic spectrum
  JOURNAL   Eur J Hum Genet 29 (7), 1121-1128 (2021)
   PUBMED   33772159
  REMARK    GeneRIF: Novel bi-allelic variants expand the SPTBN4-related
            genetic and phenotypic spectrum.
REFERENCE   3  (bases 1 to 8910)
  AUTHORS   Fujishima,K., Kurisu,J., Yamada,M. and Kengaku,M.
  TITLE     betaIII spectrin controls the planarity of Purkinje cell dendrites
            by modulating perpendicular axon-dendrite interactions
  JOURNAL   Development 147 (24), 226102 (2020)
   PUBMED   33234719
  REMARK    GeneRIF: betaIII spectrin controls the planarity of Purkinje cell
            dendrites by modulating perpendicular axon-dendrite interactions.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8910)
  AUTHORS   Wang,C.C., Ortiz-Gonzalez,X.R., Yum,S.W., Gill,S.M., White,A.,
            Kelter,E., Seaver,L.H., Lee,S., Wiley,G., Gaffney,P.M.,
            Wierenga,K.J. and Rasband,M.N.
  TITLE     betaIV Spectrinopathies Cause Profound Intellectual Disability,
            Congenital Hypotonia, and Motor Axonal Neuropathy
  JOURNAL   Am J Hum Genet 102 (6), 1158-1168 (2018)
   PUBMED   29861105
  REMARK    GeneRIF: bi-allelic pathogenic SPTBN4 variants (three homozygous
            and two compound heterozygous) that cause a severe neurological
            syndrome that includes congenital hypotonia, intellectual
            disability, and motor axonal and auditory neuropathy, are reported.
REFERENCE   5  (bases 1 to 8910)
  AUTHORS   Yoshida,T., Kato,K., Yokoi,K., Oguri,M., Watanabe,S., Metoki,N.,
            Yoshida,H., Satoh,K., Aoyagi,Y., Nozawa,Y. and Yamada,Y.
  TITLE     Association of genetic variants with hemorrhagic stroke in Japanese
            individuals
  JOURNAL   Int J Mol Med 25 (4), 649-656 (2010)
   PUBMED   20198315
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 8910)
  AUTHORS   Komada,M. and Soriano,P.
  TITLE     [Beta]IV-spectrin regulates sodium channel clustering through
            ankyrin-G at axon initial segments and nodes of Ranvier
  JOURNAL   J Cell Biol 156 (2), 337-348 (2002)
   PUBMED   11807096
REFERENCE   7  (bases 1 to 8910)
  AUTHORS   Tse,W.T., Tang,J., Jin,O., Korsgren,C., John,K.M., Kung,A.L.,
            Gwynn,B., Peters,L.L. and Lux,S.E.
  TITLE     A new spectrin, beta IV, has a major truncated isoform that
            associates with promyelocytic leukemia protein nuclear bodies and
            the nuclear matrix
  JOURNAL   J Biol Chem 276 (26), 23974-23985 (2001)
   PUBMED   11294830
REFERENCE   8  (bases 1 to 8910)
  AUTHORS   Berghs,S., Aggujaro,D., Dirkx,R. Jr., Maksimova,E., Stabach,P.,
            Hermel,J.M., Zhang,J.P., Philbrick,W., Slepnev,V., Ort,T. and
            Solimena,M.
  TITLE     betaIV spectrin, a new spectrin localized at axon initial segments
            and nodes of ranvier in the central and peripheral nervous system
  JOURNAL   J Cell Biol 151 (5), 985-1002 (2000)
   PUBMED   11086001
REFERENCE   9  (bases 1 to 8910)
  AUTHORS   Chardin,P., Camonis,J.H., Gale,N.W., van Aelst,L., Schlessinger,J.,
            Wigler,M.H. and Bar-Sagi,D.
  TITLE     Human Sos1: a guanine nucleotide exchange factor for Ras that binds
            to GRB2
  JOURNAL   Science 260 (5112), 1338-1343 (1993)
   PUBMED   8493579
REFERENCE   10 (bases 1 to 8910)
  AUTHORS   Ortiz-Gonzalez,X. and Wierenga,K.
  TITLE     SPTBN4 Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   32672909
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC020929.6, AF311855.1,
            AB046862.1 and AI962525.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_020971.2.
            
            Summary: Spectrin is an actin crosslinking and molecular scaffold
            protein that links the plasma membrane to the actin cytoskeleton,
            and functions in the determination of cell shape, arrangement of
            transmembrane proteins, and organization of organelles. It is
            composed of two antiparallel dimers of alpha- and beta- subunits.
            This gene is one member of a family of beta-spectrin genes. The
            encoded protein localizes to the nuclear matrix, PML nuclear
            bodies, and cytoplasmic vesicles. A highly similar gene in the
            mouse is required for localization of specific membrane proteins in
            polarized regions of neurons. Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (sigma1) represents the longer
            transcript and encodes the longer isoform (sigma1), also known as
            the 289 kDa isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF311855.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145245, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000598249.6/ ENSP00000469242.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-218               AC020929.6         3476-3693
            219-1231            AF311855.1         1-1013
            1232-1232           AC020929.6         38617-38617
            1233-2455           AF311855.1         1015-2237
            2456-7467           AB046862.1         981-5992
            7468-8238           AF311855.1         7250-8020
            8239-8680           AC020929.6         112267-112708
            8681-8905           AI962525.1         23-247              c
            8906-8910           AC020929.6         112934-112938
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.2"
     gene            1..8910
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /note="spectrin beta, non-erythrocytic 4"
                     /db_xref="GeneID:57731"
                     /db_xref="HGNC:HGNC:14896"
                     /db_xref="MIM:606214"
     exon            1..305
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            306..489
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     CDS             321..8015
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /note="isoform sigma1 is encoded by transcript variant
                     sigma1; spectrin beta chain, brain 3; beta-IV spectrin;
                     spectrin, non-erythroid beta chain 3; spectrin beta chain,
                     non-erythrocytic 4"
                     /codon_start=1
                     /product="spectrin beta chain, non-erythrocytic 4 isoform
                     sigma1"
                     /protein_id="NP_066022.2"
                     /db_xref="CCDS:CCDS12559.1"
                     /db_xref="GeneID:57731"
                     /db_xref="HGNC:HGNC:14896"
                     /db_xref="MIM:606214"
                     /translation="MAQVPGEVDNMEGLPAPNNNPAARWESPDRGWEREQPAASTAAA
                     SLFECSRIKALADEREAVQKKTFTKWVNSHLARVGCHIGDLYVDLRDGFVLTRLLEVL
                     SGEQLPRPTRGRMRIHSLENVDKALQFLKEQRVHLENVGSHDIVDGNHRLTLGLVWTI
                     ILRFQIQVIKIETEDNRETRSAKDALLLWCQMKTAGYPEVNIQNFTTSWRDGLAFNAL
                     IHRHRPDLVDFSKLTKSNANYNLQRAFRTAEQHLGLARLLDPEDVNMEAPDEKSIITY
                     VVSFYHYFSKMKALAVEGKRIGKVLDQVLEVGKIIERYEELAAELLAWIHRTVGLISN
                     QKFANSLSGVQQQLQAFTAYCTLEKPVKFQEKGNLEVLLFSIQSKLRACNRRLFVPRE
                     GCGIWDIDKAWGELEKAEHEREAALRAELIRQEKLELLAQRFDHKVAMRESWLNENQR
                     LVSQDNFGYELPAVEAAMKKHEAIEADIAAYEERVQGVAELAQALAAEGYYDIRRVAA
                     QRDSVLRQWALLTGLVGARRTRLEQNLALQKVFQEMVYMVDWMEEMQAQLLSRECGQH
                     LVEADDLLQKHGLLEGDIAAQSERVEALNAAALRFSQLQGYQPCDPQVICNRVNHVHG
                     CLAELQEQAARRRAELEASRSLWALLQELEEAESWARDKERLLEAAGGGGAAGAAGAA
                     GTAGGAHDLSSTARLLAQHKILQGELGGRRALLQQALRCGEELVAAGGAVGPGADTVH
                     LVGLAERAASARRRWQRLEEAAARRERRLQEARALHQFGADLDGLLDWLRDAYRLAAA
                     GDFGHDEASSRRLARQHRALTGEVEAHRGPVSGLRRQLATLGGASGAGPLVVALQVRV
                     VEAEQLFAEVTEVAALRRQWLRDALAVYRMFGEVHACELWIGEKEQWLLSMRVPDSLD
                     DVEVVQHRFESLDQEMNSLMGRVLDVNHTVQELVEGGHPSSDEVRSCQDHLNSRWNRI
                     VELVEQRKEEMSAVLLVENHVLEVAEVRAQVREKRRAVESAPRAGGALQWRLSGLEAA
                     LQALEPRQAALLEEAALLAERFPAQAARLHQGAEELGAEWGALASAAQACGEAVAAAG
                     RLQRFLHDLDAFLDWLVRAQEAAGGSEGPLPNSLEEADALLARHAALKEEVDQREEDY
                     ARIVAASEALLAADGAELGPGLALDEWLPHLELGWHKLLGLWEARREALVQAHIYQLF
                     LRDLRQALVVLRNQEMALSGAELPGTVESVEEALKQHRDFLTTMELSQQKMQVAVQAA
                     EGLLRQGNIYGEQAQEAVTRLLEKNQENQLRAQQWMQKLHDQLELQHFLRDCHELDGW
                     IHEKMLMARDGTREDNHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQEKPELA
                     ASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKKLLHMESQLQDV
                     DPGGDLATVNSQLKKLQSMESQVEEWYREVGELQAQTAALPLEPASKELVGERQNAVG
                     ERLVRLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGNGLQAVQQ
                     HIKKNQGLRREIQAHGPRLEEVLERAGALASLRSPEAEAVRRGLEQLQSAWAGLREAA
                     ERRQQVLDAAFQVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQ
                     GVENYEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDRLYVALKELGEERRVALEQQ
                     YWLYQLSRQVSELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEFASETGMAGRERL
                     AAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELHKFFSD
                     ARELQGQIEEKRRRLPRLTTPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQ
                     LRTVYAGEHAEAIASREQEVLQGWKELLSACEDARLHVSSTADALRFHSQVRDLLSWM
                     DGIASQIGAADKPRDVSSVEVLMNYHQGLKTELEARVPELTTCQELGRSLLLNKSAMA
                     DEIQAQLDKLGTRKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSR
                     ELGSSVDEVEQLIRRHEAFRKAAAAWEERFSSLRRLTTIEKIKAEQSKQPPTPLLGRK
                     FFGDPTELAAKAAPLLRPGGYERGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQP
                     RIDRLPEIPGRVEPAALPAAPEDAAETPATPAAAEQVRPRPERQESADRAEELPRRRR
                     PERQESVDQSEEAARRRRPERQESAEHEAAHSLTLGRYEQMERRRERRERRLERQESS
                     EQEMPIRGDLVKGKATLADIVEQLQEKEAGPGLPAGPSLPQPRELPPGRLPNGLELPE
                     RTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSRSAPAQGGSAPAPPPPPTHTVQHE
                     GFLLRKRELDANRKSSNRSWVSLYCVLSKGELGFYKDSKGPASGSTHGGEPLLSLHKA
                     TSEVASDYKKKKHVFKLQTQDGSEFLLQAKDEEEMNGWLEAVASSVAEHAEIARWGQT
                     LPTTSSTDEGNPKREGGDRRASGRRK"
     exon            490..641
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            642..815
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            816..907
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            908..988
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1104
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1217
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..1405
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1523
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1682
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1985
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2136
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..3085
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            3086..3223
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            3224..3974
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            3975..4177
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            4178..4268
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            4269..4415
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            4416..4679
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            4680..4904
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            4905..4994
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            4995..5273
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            5274..5404
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            5405..5609
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            5610..5990
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            5991..6235
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            6236..6374
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            6375..6459
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            6460..6656
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            6657..7276
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            7277..7346
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            7347..7639
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            7640..7813
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            7814..7856
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     exon            7857..8910
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8886..8891
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /note="hexamer: AATAAA"
     polyA_site      8910
                     /gene="SPTBN4"
                     /gene_synonym="CMND; NEDHND; QV; SPNB4; SPTBN3"
                     /note="major polyA site"
ORIGIN      
        1 gccgtgccct cgcgagtgca gccgtgcacc ccacgccgcg gccgggtgtg actgcgcgtg
       61 ggcctcgggc ggcggggcgc ggggaccgcg ggcgggaggg ggtcccgggg gcgcgctgag
      121 cgcggcggcg gcgcgagaga gggaggcgcg gcggcgcatg cggatctggc tgagccgcgg
      181 gccggcgggg ccgagctgag ccgggctggg ccgggccggg ccgggccggg cagcggacgc
      241 cgacagggag ggcggtcggt cgcggcgagc agcggagaca gcgacgctgg cggccgcggg
      301 cccaggcctc accttccccg atggcgcagg taccagggga agtggacaac atggagggcc
      361 tgcctgctcc taacaacaac cctgctgccc gctgggagag tccggatcgg ggctgggagc
      421 gggagcagcc ggctgcgtcc accgcagcgg cctcgctctt tgagtgctcc cggatcaagg
      481 ccttggcaga tgagcgggaa gccgtgcaga agaaaacctt caccaagtgg gtgaactcgc
      541 acctcgcccg cgtgggctgc cacatcgggg acctctatgt ggacctccgg gacggcttcg
      601 tgctcacgcg gctcctggaa gtgctgtctg gggagcagct gcccaggccc acgcgcggcc
      661 gcatgcggat ccactcactg gagaacgtgg acaaggcgct gcagtttctg aaggagcagc
      721 gcgtgcacct ggagaacgtg ggttcgcatg acatcgtgga tgggaatcac cggctgacgc
      781 tggggctggt ctggaccatc atcctgcgct tccagattca agtcatcaaa attgagactg
      841 aggacaacag agagacacgc tcagccaagg atgctctgct cttgtggtgt cagatgaaga
      901 cagctggtta ccctgaggta aacatccaga atttcaccac cagctggcgg gatggcttgg
      961 ccttcaatgc cctcattcac cggcacaggc ctgatctcgt ggacttcagc aaactcacca
     1021 agtccaatgc caactacaac ctgcagagag ccttccgcac agctgagcag cacctggggc
     1081 tggcgcggct gctggatcct gaagatgtga acatggaggc tccagatgag aagtccatca
     1141 tcacctacgt ggtctctttc taccactatt tctccaagat gaaggctctg gctgtggagg
     1201 ggaagcgtat cgggaaggtc ttggaccagg tattggaggt ggggaagatc atagaacgct
     1261 acgaggagct ggcggctgag ctgctggcct ggatccaccg caccgtgggc ctcatcagca
     1321 atcagaaatt tgccaactcc ttaagtgggg tgcagcagca actccaggct ttcacggcct
     1381 attgcacgct ggagaagcct gtcaagttcc aggagaaggg gaacctagag gtgctgctct
     1441 tcagcatcca gagcaaactg cgtgcctgca accgtcgcct ctttgtgcct cgggagggct
     1501 gtggcatctg ggatattgac aaggcatggg gtgagctgga gaaggctgag catgagcggg
     1561 aggctgccct acgggctgag ctgattcggc aggagaagct ggaactactg gcacagaggt
     1621 ttgaccacaa ggtggctatg agggagagct ggctgaatga gaaccagcgt ctggtctccc
     1681 aggacaactt tgggtatgag ctgcccgcag tggaggcagc catgaagaaa cacgaagcga
     1741 tcgaggcaga cattgcggcc tacgaggagc gggtgcaggg tgtggcggag ctggcccagg
     1801 cattggcagc cgaaggctac tacgatatcc ggcgggtggc agcccagcgt gacagcgtcc
     1861 tgcgccagtg ggccctgcta actgggcttg tgggtgcccg gcggacacga cttgagcaga
     1921 accttgccct gcagaaggtc ttccaggaga tggtgtacat ggtggactgg atggaggaga
     1981 tgcaggctca gctgctgtcc cgggagtgtg ggcagcacct ggtggaggca gacgacctgt
     2041 tgcagaagca tggactgctg gagggagaca ttgccgccca gagcgagcgg gtggaggctc
     2101 tcaatgccgc tgccctgcgc ttctcccagc tgcagggcta ccagccctgc gacccgcagg
     2161 tcatctgcaa ccgcgtgaac cacgtgcacg gctgcctggc ggagctgcag gagcaggcag
     2221 cgcggcgacg cgcggagctg gaggcttcgc ggagcctgtg ggcgctgctg caggagctgg
     2281 aggaggccga gagctgggcg cgcgacaagg agcgtctcct ggaggctgcg ggcggcggcg
     2341 gtgcggcggg cgcagcgggc gcagcgggaa cagcgggcgg cgcgcatgac ctgtccagca
     2401 cagcgcgcct cctggcccag cacaagatcc tgcagggcga gctgggcggg cggcgagcgt
     2461 tgctgcagca ggccctgcgg tgtggcgagg agctggttgc ggccggcggt gccgtcggcc
     2521 cgggagcaga caccgtgcac ctggtaggcc tggcggagcg cgcggcgagc gcccggcgcc
     2581 gctggcagag gctggaagag gcggcggcgc ggcgagagcg gcggctgcag gaggcgcggg
     2641 cgctgcacca gttcggcgct gacctcgacg ggctgctgga ctggcttcgc gacgcttacc
     2701 gcctggcagc cgccggtgac ttcggccacg acgaagcttc cagccgccgc ctggcgcgcc
     2761 agcaccgcgc gctcaccggg gaggtggagg cacatcgcgg gcccgtgagc ggcctgcggc
     2821 gccagctggc gacactcggg ggtgccagtg gcgcagggcc actggtggtg gcgctgcagg
     2881 tgcgcgtggt ggaagcagag cagttgttcg ctgaggtgac cgaagtggcg gcgctgaggc
     2941 gccagtggct gcgggacgcg ctcgctgtct accgcatgtt tggcgaggtg cacgcgtgtg
     3001 agctgtggat cggcgagaag gagcaatggc tgctctccat gcgtgtgccg gattcactcg
     3061 acgacgtcga ggtggtgcag caccgattcg agagcctgga ccaagagatg aacagcctga
     3121 tgggccgcgt tctggacgtg aaccacacag tccaggagct ggtggaagga ggccacccca
     3181 gttcagatga ggtgcgttcc tgccaggacc acctcaacag caggtggaac cgcatcgtgg
     3241 agctagtgga acagcgcaaa gaggaaatga gcgcggtgct gctggtggag aaccacgtgc
     3301 tggaggtggc cgaggtgcgc gcccaggtgc gtgagaagcg gagagctgtg gagagcgcgc
     3361 cccgggccgg cggcgccctg cagtggcgtc ttagcggcct agaggccgct ctgcaggcgc
     3421 tggagccgcg ccaggcggcc cttctggagg aggcagccct gctggctgag cgcttcccgg
     3481 cgcaggcggc gcggctgcac cagggcgcgg aggagctggg cgccgagtgg ggcgcgctag
     3541 ctagcgcggc tcaggcctgc ggcgaggcgg tggcggcagc agggcgcctg cagcgcttcc
     3601 tacatgacct cgacgctttc ctggactggc tcgtgcgcgc ccaggaggcg gcgggcggca
     3661 gcgaggggcc cctgcccaac agcctagaag aggcggacgc gctgctggcg cgccacgctg
     3721 cgctcaagga ggaggtggac cagcgcgagg aagactatgc tcgcatcgtg gcggccagcg
     3781 aggcgctgct ggccgccgac ggcgcagagc tgggcccggg cctggcacta gacgagtggc
     3841 tgccacacct cgaacttggc tggcataaac tgctcggctt gtgggaggcg cgcagggagg
     3901 cgctggtcca ggcgcacatc taccagctct tcctgcggga tctacgccag gcgctcgtgg
     3961 tgctgcgtaa ccaggagatg gcgctgtctg gtgcggagct cccgggcaca gtggaatcgg
     4021 tggaggaggc cttgaaacag caccgtgact ttctcaccac catggagctg agccagcaaa
     4081 agatgcaggt ggccgtgcag gctgcagagg gcctgctgag gcagggcaac atctacgggg
     4141 agcaggctca ggaggctgtg acccggctgc tggagaagaa ccaagaaaac cagttacggg
     4201 cccagcaatg gatgcaaaag ctacatgacc aacttgagct gcagcacttc ctccgagact
     4261 gccacgagct ggatggctgg atccatgaga agatgctgat ggcgcgggat ggcacgcggg
     4321 aggacaacca caagctgcat aagagatggc tccggcacca ggcattcatg gccgagctgg
     4381 ctcagaataa ggagtggctg gagaagatcg agcgggaggg ccagcaactg atgcaggaga
     4441 agcccgaact ggcggcctcc gtgcggaaga agctgggcga gatccgccag tgctgggcgg
     4501 agctggagag caccacccag gccaaggcac ggcagctctt tgaggccagc aaagcagacc
     4561 agctggtgca gagctttgct gagctggaca agaagctcct tcacatggag agccagctgc
     4621 aagacgtgga ccctggagga gacctggcca ctgtcaacag tcagctcaag aagctgcagt
     4681 ccatggagtc gcaggtggag gagtggtacc gcgaggtggg agagctgcag gcgcagacgg
     4741 cggcgctgcc gctggagccg gcgagcaagg agctggtggg tgagcggcag aacgcggtgg
     4801 gcgagcgcct ggtgcgcctg ctcgagccgt tgcaggagcg ccgccgcttg ctgctggctt
     4861 ccaaggagtt gcaccaggtg gcgcacgacc tggacgacga gctggcatgg gttcaggagc
     4921 ggctgccact ggccatgcag acagagcgag gcaacggttt gcaggcggtc cagcagcaca
     4981 tcaaaaagaa ccagggcctg cggcgggaga tccaggcgca tgggccgcgc ctggaggagg
     5041 tgctggagcg cgcgggcgcg ctggcgtcgc tgcgcagccc ggaggcagag gcagtgcgcc
     5101 ggggcctgga gcagctgcag agcgcctggg ccggactgcg ggaggctgcc gagcgacggc
     5161 agcaggtgct ggacgccgcc ttccaggtgg agcagtacta cttcgacgtg gctgaggtgg
     5221 aggcgtggct gggcgagcag gagctgctca tgatgagtga ggacaagggc aaggacgaac
     5281 agagcaccct gcagctgctc aagaaacacc tgcagctgga gcaaggcgtg gagaactacg
     5341 aggaaagcat cgcgcagctg tcgcgccagt gccgggcgct gctggagatg gggcacccgg
     5401 acagcgagca gatcagccgg cggcagtctc aggtggaccg cctgtacgtg gcgctcaagg
     5461 agctgggtga ggagcgccgg gtggctctgg aacagcagta ctggctgtac cagctcagcc
     5521 gccaggtgag cgagcttgag cactggattg ccgagaagga ggtggtggct ggctcacccg
     5581 agctcggcca ggactttgag catgtctcgg tgctgcagga gaaattctca gagtttgcca
     5641 gcgagacagg tatggcaggg cgggaacggc tggcagctgt gaaccagatg gtggatgagc
     5701 tgatcgagtg tggccataca gcagcggcca ccatggccga gtggaaggac ggactgaacg
     5761 aggcctgggc tgagctgctg gagctcatgg gcacacgggc ccagctgctg gccgcctctc
     5821 gggagcttca taagttcttc agtgacgccc gagagcttca gggacagatt gaggagaagc
     5881 ggaggcggct gccccgcctg accaccccgc ctgagccgag acccagtgcc agttccatgc
     5941 agcggaccct gagagccttt gagcatgacc tgcagctcct cgtgtcccag gtacggcagc
     6001 tgcaggaggg ggcggcccag ctgcggacgg tgtatgcggg tgaacatgcc gaggccatcg
     6061 ctagccggga gcaggaggtg ctgcagggtt ggaaagagct gctgtcagcc tgtgaggatg
     6121 cccgcctgca tgtcagctcc acagccgacg ccctgcgctt ccacagccaa gtccgcgacc
     6181 tgctctcctg gatggatggc atcgccagcc agattggggc agccgacaag cccagggacg
     6241 tgtcatcagt ggaggtgctc atgaactacc accagggcct gaagactgag ctggaggcgc
     6301 gggtgcctga gctgaccacc tgccaggagc tggggcgatc tctgctgctc aacaaaagtg
     6361 ccatggctga tgagatccag gcacagctgg acaagctggg aaccaggaag gaggaggtgt
     6421 cggaaaagtg ggaccgccat tgggagtggc tgcagcagat gctggaggtg caccagtttg
     6481 cccaggaggc ggtggtggct gatgcctggc tgacagccca ggagccgctc ctgcagagcc
     6541 gggagctggg cagcagcgtg gatgaggtgg agcagcttat ccggcgacat gaggccttcc
     6601 gcaaagcggc tgcagcctgg gaagagaggt tcagctctct gcggcgcctg accacgatcg
     6661 agaaaatcaa agcggaacag agcaagcagc cgcctacccc actgctgggg cgcaagttct
     6721 ttggggaccc cacggaactg gcggccaagg cggcgcccct gctgcggcca gggggctatg
     6781 aaaggggctt ggagcccctg gcccgccgag cctcggacac gctctcggcc gaggtgcgga
     6841 ctcgggtggg gtatgtgcgc caggagctca agcccgagcg cctccagccg cgcattgacc
     6901 ggctgccgga gatcccgggg agggtggagc ccgcggccct gccggccgca ccagaggacg
     6961 cggcggagac ccccgcgacc cccgcggcgg cggagcaggt gcggccacga ccggagcgcc
     7021 aggagtcagc tgatcgcgcg gaggagctgc ccaggaggcg gcggcctgag cggcaagagt
     7081 cagtcgatca atccgaggag gctgcgcgga ggcggcggcc ggagcggcag gagtcagcgg
     7141 agcacgaggc ggcacacagc cttaccctgg gccgctatga gcagatggag cggcggcgcg
     7201 agcggcgtga gcggcgcttg gagcggcagg agtccagcga acaggagatg cccatcagag
     7261 gagacctggt caaggggaag gccaccctgg ctgacattgt ggaacagctg caggagaaag
     7321 aggcaggccc agggctgcct gctgggccgt cgctgcctca gccacgcgag cttcccccag
     7381 gtcgcctgcc caacgggctt gagctgcccg agcggacacc tcggccggac cggccccggg
     7441 cgcgggaccg gcccaagccg cgacggcggc cgcggcccag agagggtggt gagggcgggg
     7501 gaagccggcg ctcgcgctcc gccccggccc agggcggctc cgcccccgcg cctccgccac
     7561 cgcccactca cacagtgcag cacgagggct tcctactgcg caagcgcgag ctcgacgcta
     7621 accgcaagtc gtccaaccgg tcgtgggtga gcctgtactg tgtgcttagt aagggggaac
     7681 tgggcttcta caaggactcc aagggcccgg catccgggag cacacacggt ggggaaccgc
     7741 tgctcagcct gcacaaggcc accagcgagg tggctagtga ctacaagaaa aagaagcatg
     7801 tcttcaagct ccagacccag gatggcagtg agtttttgct ccaggcaaaa gatgaggagg
     7861 agatgaacgg ctggctggag gctgtagctt cctcggtggc ggaacacgca gagatcgccc
     7921 gctggggcca gacactaccc actacttcat ccacagatga gggcaaccct aagagggaag
     7981 gcggagatcg cagggccagc gggcgcagga agtgacttcc cacccccagg acctgacaca
     8041 tctcgtctcc cctcttttcc gcactgtggg cacaaagaca ctttttcttc cgcaggggcg
     8101 ggagccccta gttccaacac tgaggacgcg tgacatggtg ggcaccggaa aggaggggac
     8161 ttctcctgca ccccaagaag tggtggggag attgctgccc ctatagccat atctcggccc
     8221 cttcccactc accaccccca ccccaggtgc tgggggtccc ttatttttat gcaataactg
     8281 agcttgatgg gggtgggcag ggggccagtt gagccaagcc cccagccccg atctgcagat
     8341 cctgccccaa gaagctgggg tggtgggggc agtaattcct gcccccctct ctgccctagg
     8401 gatgggcacg ggggcgctgg tgaggtcccc tggaccatcc agggtgctag ggggcaggga
     8461 ggggacaccc cctccccgcc tttacctcac ttccaatgct gccttgatct ctgtctggga
     8521 ggggggagtg aaggggccct agccccctgc actccgccgc ctcagagcca tgcggttaat
     8581 tcctgactta gtttattttt gcaaaacgtc gatctcctcc tcccccgccc cgcatccgcg
     8641 aaggctttta atgggagggg cgtcaaagct caaaactgtt ttcctctctc ctcccccctc
     8701 cagttgtaaa tgccacttca tgaggggagg ggcgagggga agcccacccc tgcatgcttc
     8761 tggctggagc acctccctgg ggagtcgggg gattgggttg tgggcagtcc ccatggccgc
     8821 ctggagaagc cgctggggcc cgggggtgtg gggcggtgtg gcgggcgcac actgtatgta
     8881 cctataataa accctttggc tttgacggtc
//
