LOCUS       NM_006946              11136 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript
            variant 1, mRNA.
ACCESSION   NM_006946 XM_005274192
VERSION     NM_006946.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Deng,J., Lin,X., Qin,J., Li,Q., Zhang,Y., Zhang,Q., Ji,C., Shen,S.,
            Li,Y., Zhang,B. and Lin,N.
  TITLE     SPTBN2 suppresses ferroptosis in NSCLC cells by facilitating
            SLC7A11 membrane trafficking and localization
  JOURNAL   Redox Biol 70, 103039 (2024)
   PUBMED   38241838
  REMARK    GeneRIF: SPTBN2 suppresses ferroptosis in NSCLC cells by
            facilitating SLC7A11 membrane trafficking and localization.
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   Chen,C., Zhang,Q., Wang,B., Song,Y., Feng,Z. and Ren,S.
  TITLE     SPTBN2 regulated by miR-214-3p inhibits the proliferation and
            migration of colorectal cancer cells
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 69 (14), 126-131 (2023)
   PUBMED   38279463
  REMARK    GeneRIF: SPTBN2 regulated by miR-214-3p inhibits the proliferation
            and migration of colorectal cancer cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11136)
  AUTHORS   Atang,A.E., Keller,A.R., Denha,S.A. and Avery,A.W.
  TITLE     Increased Actin Binding Is a Shared Molecular Consequence of
            Numerous SCA5 Mutations in beta-III-Spectrin
  JOURNAL   Cells 12 (16), 2100 (2023)
   PUBMED   37626910
  REMARK    GeneRIF: Increased Actin Binding Is a Shared Molecular Consequence
            of Numerous SCA5 Mutations in beta-III-Spectrin.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11136)
  AUTHORS   Perkins,E., Suminaite,D. and Jackson,M.
  TITLE     Cerebellar ataxias: beta-III spectrin's interactions suggest common
            pathogenic pathways
  JOURNAL   J Physiol 594 (16), 4661-4676 (2016)
   PUBMED   26821241
  REMARK    Review article
REFERENCE   5  (bases 1 to 11136)
  AUTHORS   Zhang,R., Zhang,C., Zhao,Q. and Li,D.
  TITLE     Spectrin: structure, function and disease
  JOURNAL   Sci China Life Sci 56 (12), 1076-1085 (2013)
   PUBMED   24302288
  REMARK    Review article
REFERENCE   6  (bases 1 to 11136)
  AUTHORS   De Matteis,M.A. and Morrow,J.S.
  TITLE     Spectrin tethers and mesh in the biosynthetic pathway
  JOURNAL   J Cell Sci 113 (Pt 13), 2331-2343 (2000)
   PUBMED   10852813
  REMARK    Review article
REFERENCE   7  (bases 1 to 11136)
  AUTHORS   Stankewich,M.C., Tse,W.T., Peters,L.L., Ch'ng,Y., John,K.M.,
            Stabach,P.R., Devarajan,P., Morrow,J.S. and Lux,S.E.
  TITLE     A widely expressed betaIII spectrin associated with Golgi and
            cytoplasmic vesicles
  JOURNAL   Proc Natl Acad Sci U S A 95 (24), 14158-14163 (1998)
   PUBMED   9826670
REFERENCE   8  (bases 1 to 11136)
  AUTHORS   Ohara,O., Ohara,R., Yamakawa,H., Nakajima,D. and Nakayama,M.
  TITLE     Characterization of a new beta-spectrin gene which is predominantly
            expressed in brain
  JOURNAL   Brain Res Mol Brain Res 57 (2), 181-192 (1998)
   PUBMED   9675416
REFERENCE   9  (bases 1 to 11136)
  AUTHORS   Ranum,L.P., Schut,L.J., Lundgren,J.K., Orr,H.T. and Livingston,D.M.
  TITLE     Spinocerebellar ataxia type 5 in a family descended from the
            grandparents of President Lincoln maps to chromosome 11
  JOURNAL   Nat Genet 8 (3), 280-284 (1994)
   PUBMED   7874171
REFERENCE   10 (bases 1 to 11136)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001157.4 and AB008567.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On or before Dec 17, 2019 this sequence version replaced
            XM_005274192.4, NM_006946.3.
            
            Summary: Spectrins are principle components of a cell's
            membrane-cytoskeleton and are composed of two alpha and two beta
            spectrin subunits. The protein encoded by this gene (SPTBN2), is
            called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is
            related to, but distinct from, the beta-II spectrin gene which is
            also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2
            regulates the glutamate signaling pathway by stabilizing the
            glutamate transporter EAAT4 at the surface of the plasma membrane.
            Mutations in this gene cause a form of spinocerebellar ataxia,
            SCA5, that is characterized by neurodegeneration, progressive
            locomotor incoordination, dysarthria, and uncoordinated eye
            movements. [provided by RefSeq, Dec 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.3880190.1,
                                           SRR14372079.1992859.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000533211.6/ ENSP00000432568.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-476               AP001157.4         147845-148320       c
            477-517             AP001157.4         140504-140544       c
            518-3061            AB008567.1         1-2544
            3062-3062           AP001157.4         123907-123907       c
            3063-8381           AB008567.1         2546-7864
            8382-11136          AP001157.4         101601-104355       c
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.2"
     gene            1..11136
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /note="spectrin beta, non-erythrocytic 2"
                     /db_xref="GeneID:6712"
                     /db_xref="HGNC:HGNC:11276"
                     /db_xref="MIM:604985"
     exon            1..476
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            477..567
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            568..746
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     CDS             590..7762
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /note="isoform 1 is encoded by transcript variant 1;
                     spectrin beta chain, brain 2; beta-III spectrin; glutamate
                     transporter EAAT4-associated protein 41; spectrin,
                     non-erythroid beta chain 2; spinocerebellar ataxia 5
                     protein; spectrin beta chain, non-erythrocytic 2; spectrin
                     beta III sigma 2"
                     /codon_start=1
                     /product="spectrin beta chain, non-erythrocytic 2 isoform
                     1"
                     /protein_id="NP_008877.2"
                     /db_xref="CCDS:CCDS8150.1"
                     /db_xref="GeneID:6712"
                     /db_xref="HGNC:HGNC:11276"
                     /db_xref="MIM:604985"
                     /translation="MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFE
                     RSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGEI
                     LPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRF
                     QIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKH
                     RPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATY
                     YHYFSKMKALAVEGKRIGKVLDHAMEAERLVEKYESLASELLQWIEQTIVTLNDRQLA
                     NSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLI
                     SDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQ
                     DNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAERYHDIKRIAARQHN
                     VARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMEEMKGRLQSQDLGRHLAGV
                     EDLLQLHELVEADIAVQAERVRAVSASALRFCNPGKEYRPCDPQLVSERVAKLEQSYE
                     ALCELAAARRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGALRLLNK
                     HTALRGEMSGRLGPLKLTLEQGQQLVAEGHPGASQASARAAELQAQWERLEALAEERA
                     QRLAQAASLYQFQADANDMEAWLVDALRLVSSPELGHDEFSTQALARQHRALEEEIRS
                     HRPTLDALREQAAALPPTLSRTPEVQSRVPTLERHYEELQARAGERARALEAALALYT
                     MLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNTLAAQITAVNDI
                     AEQLLKANPPGKDRIVNTQEQLNHRWQQFRRLADGKKAALTSALSIQNYHLECTETQA
                     WMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAIAARVGELTREANALAAGHP
                     AQAVAINARLREVQTGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAV
                     ASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRALGEEVTRDQADPQCLFLRQR
                     LEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTL
                     QAADAAIKKLEDFMSTMDANGERIHGLLEAGRQLVSEGNIHADKIREKADSIERRHKK
                     NQDAAQQFLGRLRDNREQQHFLQDCHELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQ
                     AFMAELAANKDWLDKVDKEGRELTLEKPELKALVSEKLRDLHRRWDELETTTQAKARS
                     LFDANRAELFAQSCCALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEWEMAVRE
                     KEVEAIQAQAKALAQEDQGAGEVERTSRAVEEKFRALCQPMRERCRRLQASREQHQFH
                     RDVEDEILWVTERLPMASSMEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLRERQR
                     ALGAAAAGPELAELQEMWKRLGHELELRGKRLEDALRAQQFYRDAAEAEAWMGEQELH
                     MMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIHQLAASSQDMIDHEHPESTRISIR
                     QAQVDKLYAGLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDY
                     EHVTMLRDKFREFSRDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWA
                     DLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRH
                     CAYEHDIQALSPQVQQVQDDGHRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARR
                     QLLLDTTDKFRFFKAVRELMLWMDEVNLQMDAQERPRDVSSADLVIKNQQGIKAEIEA
                     RADRFSSCIDMGKELLARSHYAAEEISEKLSQLQARRQETAEKWQEKMDWLQLVLEVL
                     VFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFCALEK
                     LTALEEREKERKRKREEEERRKQPPAPEPTASVPPGDLVGGQTASDTTWDGTQPRPPP
                     STQAPSVNGVCTDGEPSQPLLGQQRLEHSSFPEGPGPGSGDEANGPRGERQTRTRGPA
                     PSAMPQSRSTESAHAATLPPRGPEPSAQEQMEGMLCRKQEMEAFGKKAANRSWQNVYC
                     VLRRGSLGFYKDAKAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEY
                     LFQAKDEAEMSSWLRVVNAAIATASSASGEPEEPVVPSTTRGMTRAMTMPPVSPVGAE
                     GPVVLRSKDGREREREKRFSFFKKNK"
     exon            747..898
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1072
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1164
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1245
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1361
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1474
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1662
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1780
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            1781..1939
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2242
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2396
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            2397..3267
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            3268..3405
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            3406..4162
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            4163..4365
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            4366..4456
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            4457..4603
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            4604..4867
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            4868..5092
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            5093..5182
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            5183..5443
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            5444..5574
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            5575..5779
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            5780..6154
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            6155..6399
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            6400..6538
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            6539..6623
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            6624..6820
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            6821..6963
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            6964..7039
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            7040..7090
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            7091..7311
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            7312..7485
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            7486..7528
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     exon            7529..11136
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8362..8367
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /note="hexamer: ATTAAA"
     polyA_site      8389
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /note="major polyA site"
     regulatory      11117..11122
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /note="hexamer: ATTAAA"
     polyA_site      11136
                     /gene="SPTBN2"
                     /gene_synonym="GTRAP41; SCA5; SCAR14"
                     /note="major polyA site"
ORIGIN      
        1 ataattcctt cctctcctaa gcaggcagca gttcaccttg gttaaggacc ttggacctta
       61 gaccactcag acgctaacaa accaaggaga aacccagccg tgtcaggcct agaaatgtct
      121 gcccccaccc ccacccccct caagacggtg actaggccgg aattctcctg gttgataaat
      181 gagaggagct gagaagcgct gagatgcctg tttggagcat ctgatctgta gaagacgaat
      241 cttcactgtg ccatggagat gttctgggag ggagagccgg cccagctgcc tgaaggagga
      301 cgcgagagcg aggggacggg tgagaggaat gcttagcgga gacggactgg aggtgcccgg
      361 agaggaaagg ggaacggagg ctggatctcg ggaaacctcg tcggcagtgt cattagagga
      421 aggatcgcgt gtctcgggga tacggatttt ggaggaaaag cagaataggg acaaaggctt
      481 cccaggtggc ttcccctctt cccctggggg ctgatttcca ctgagcagcc aaccgcagcc
      541 tctggccaca aggagagcgg agcacaggag caggaagccg cctaccacca tgagcagcac
      601 gctgtcaccc acagactttg acagcttgga aatccagggc cagtacagtg acatcaacaa
      661 ccgctgggac cttcctgact cggactggga caatgacagc agctcggccc gcctctttga
      721 gaggtctcgc attaaggctc tggcagatga acgagaagct gtgcagaaga aaaccttcac
      781 caagtgggta aactcgcacc tggcccgggt cacgtgccgg gtgggggacc tgtacagcga
      841 cctccgggac ggacgcaacc tgctgaggct cctcgaggtg ctctcgggag agatactgcc
      901 aaagcctaca aagggccgca tgcggatcca ctgcctggag aacgtggaca aggcactgca
      961 gttcctcaag gagcagaaag tgcacttgga aaacatgggc tcccatgaca ttgtggacgg
     1021 aaaccaccga ctgacccttg ggctggtctg gaccatcatc cttcgattcc agatccaaga
     1081 catcagtgtg gagacagaag acaacaagga gaagaagtca gccaaggatg ccctgcttct
     1141 gtggtgccag atgaagactg caggttatcc caacgtcaat gtacacaact tcaccaccag
     1201 ctggagagat ggactagctt tcaacgccat cgtgcataaa caccggccag acctgctgga
     1261 ttttgagtct ctgaagaagt gtaatgcaca ctataatctg cagaatgcat tcaatctggc
     1321 tgaaaaggaa ctgggactta ccaagctgct ggatcccgaa gacgtgaatg tggaccagcc
     1381 agatgagaag tcaatcatta cctatgtggc tacttactac cattacttct ccaagatgaa
     1441 ggccctggcc gtggaaggca agagaattgg caaggtgctg gaccatgcca tggaggcaga
     1501 gcgcctggtg gagaaatacg agtccctggc ctcggagctg ctgcagtgga tcgagcaaac
     1561 gatcgtgacc ctcaatgacc ggcagttggc caactccctt agcggggtcc agaaccagct
     1621 gcagtccttc aactcctacc gcaccgtgga gaagccgccc aagtttaccg agaaagggaa
     1681 cttggaagtg ctgctcttca ccatccagag caagcttcgg gccaacaacc agaaggtcta
     1741 cacgccccgc gagggccggc tcatctcgga catcaacaag gcttgggagc ggctggagaa
     1801 ggcggagcac gagcgtgagc tggccctgcg caccgagctc atccgccagg agaagctgga
     1861 gcagctggcc gcccgcttcg accgcaaggc tgccatgcgg gagacctggc tcagcgagaa
     1921 ccagcgcctc gtgtcccagg acaactttgg gctggagctg gcagctgtcg aggcagcagt
     1981 acggaagcac gaagccattg agacggacat cgtggcctac agcggccggg tgcaggcagt
     2041 ggacgccgtg gctgcagagc tggccgccga gcgctaccac gacatcaagc gcatcgccgc
     2101 tcggcagcac aacgtggcac ggctctggga cttcttgcgg cagatggtgg ccgcccggcg
     2161 ggagcggctc ctcctcaacc tggagctgca gaaggtgttc caggacctgc tctacctcat
     2221 ggactggatg gaagagatga agggccggct gcagtctcag gacctgggca ggcacctagc
     2281 aggagtggag gacctgctgc agctgcacga gctggtggag gcagacatcg ccgtgcaggc
     2341 cgagagggtg cgggccgtca gcgcctctgc cctgcgcttc tgcaacccag ggaaagagta
     2401 tagaccttgc gacccgcagc tggtgtcgga gcgggtggcc aagctagagc agagctatga
     2461 ggcactgtgc gagttggcag cggcgcggcg ggcccggctg gaggaatcac ggcggctctg
     2521 gcgtttcctc tgggaggtgg gtgaagctga ggcctgggtg cgggagcagc agcacctcct
     2581 ggcctcagcc gacacgggcc gagacctgac cggtgccctc cgcctgctca acaagcacac
     2641 agccctgcgg ggcgagatga gcggccggct ggggcccctg aagctcaccc tggagcaggg
     2701 ccagcagttg gtggccgagg gtcaccctgg ggcaagccag gcctctgccc gtgcagctga
     2761 actccaagcc cagtgggagc ggctagaggc cctggccgag gagcgtgccc agcggctggc
     2821 ccaagccgcc agcctctacc agttccaggc cgatgcaaac gacatggagg cctggttggt
     2881 tgacgcactg cgcctggtgt ccagccccga gctggggcac gacgagttct ccacgcaggc
     2941 tctagccagg cagcatcggg ccctggagga ggagattcga agccaccggc caaccctgga
     3001 cgccttgagg gaacaggcag cagccctgcc ccccacactg agccgcacgc ccgaggtgca
     3061 gagccgggtg cccaccctgg agcggcacta cgaggagctg caggcccggg caggcgagcg
     3121 agcgcgggcc ttggaggcag ccctggcgct ctacaccatg ctcagcgagg ccggggcctg
     3181 tggactctgg gtggaggaga aggagcagtg gctcaacggg ctggccctgc ctgaacgcct
     3241 ggaggacctg gaggtcgtgc agcagaggtt cgagaccctg gagcctgaaa tgaacaccct
     3301 tgcagcacaa atcaccgcgg tgaatgacat tgccgagcag ttactgaagg ccaacccccc
     3361 aggcaaagac cgcattgtca acacccagga gcagctcaac cacaggtggc agcagtttcg
     3421 gcgtctggca gacggcaaga aggcagctct cacctcagcc ctgagcatcc agaactacca
     3481 cttagagtgc acggagaccc aggcctggat gagagagaag accaaagtca tcgagtccac
     3541 ccagggccta ggcaacgatc tggctggggt gctggccctg cagcgcaagc tggccggcac
     3601 ggagcgggac ctggaggcca tcgccgcccg ggtgggcgaa ctgactcgag aggcaaatgc
     3661 cctggctgcc ggccatcccg ctcaggcagt ggccatcaac gcccggctga gagaggtgca
     3721 gaccggctgg gaggacctca gggccaccat gcggcgtcga gaagagtcgc tgggggaggc
     3781 gcggcggctg caggacttct tgcgcagctt ggatgacttc caggcctggc taggccgcac
     3841 tcagactgct gtggcctctg aagaagggcc ggccaccctg cctgaggcag aggccctcct
     3901 ggcccaacat gcagccctgc ggggagaggt ggagcgggcc cagagcgagt atagccggct
     3961 gcgagccctg ggcgaggagg tgacccggga ccaggctgac ccccagtgcc tcttcctacg
     4021 acagcgactg gaggccctgg gaactggctg ggaggagctg ggccgaatgt gggagagccg
     4081 gcaaggtcgc ctggcccagg cccacggctt ccagggattc ctgcgggatg ctcgtcaggc
     4141 tgagggcgtg ctcagcagcc aggaatatgt tctgtctcac acggagatgc cagggacact
     4201 ccaggctgct gatgctgcca ttaaaaaact ggaggacttc atgagcacca tggacgccaa
     4261 tggggaacgg atccacgggc tcctggaggc tggccgccag ctggtatctg aaggcaacat
     4321 ccacgccgac aagattcggg aaaaggcaga ctccattgag aggaggcaca agaagaatca
     4381 agacgcagcg cagcaatttc tgggccgtct tcgggacaac cgggagcagc agcatttcct
     4441 gcaagattgt cacgagctga agctctggat cgacgagaag atgctgacag cccaggacgt
     4501 gtcctatgac gaggcccgca acctgcatac taagtggcag aagcaccagg cattcatggc
     4561 cgagctggct gccaacaaag actggctgga caaggtggac aaggaagggc gagagctcac
     4621 ccttgagaag ccagagctga aagccctggt gtcggagaag ctgagagacc tgcacaggcg
     4681 ctgggacgag ctggagacca ccacccaagc caaggcccgc agcctctttg atgccaaccg
     4741 agctgagctg tttgcccaga gctgctgtgc cctggagagc tggctggaga gcctgcaggc
     4801 ccagctgcac tcggatgact acggcaagga cctcaccagc gtcaacatcc tgctcaagaa
     4861 gcagcagatg ctggaatggg agatggctgt gagagagaag gaggtggagg caatccaggc
     4921 ccaggccaaa gcactggccc aggaggacca gggtgcaggg gaggtggaga gaacctcgag
     4981 ggccgtggag gagaagttca gggccttgtg ccagcccatg cgggaacgct gccggcgcct
     5041 gcaggcttct cgcgagcagc accagttcca ccgcgatgtg gaagatgaga ttttgtgggt
     5101 gacagagcgg ctgcccatgg ccagctccat ggagcatggc aaggacctgc ccagcgtcca
     5161 gcttctcatg aagaaaaacc agaccctgca gaaagagatt cagggccatg agccccggat
     5221 cgcggacctg agggagcggc agcgtgctct aggtgcagca gcagcaggtc cagagctggc
     5281 tgagctgcag gaaatgtgga aacgcctggg ccacgagctg gaacttcgag ggaagcgact
     5341 ggaggatgcc ctgcgagccc agcagttcta ccgcgatgcc gccgaggcgg aggcctggat
     5401 gggcgagcag gaattacaca tgatgggcca ggagaaggcc aaggatgagc tgagtgccca
     5461 ggcagaggtg aagaagcacc aggtgctgga gcaagccctg gccgactacg cgcagaccat
     5521 ccaccagctg gcggccagca gccaggacat gattgaccac gagcacccag agagcactcg
     5581 gatatccatc cgccaagccc aggtggacaa gctgtatgcc ggcctgaagg agctggctgg
     5641 agagcggcgg gagcgcctgc aggagcacct ccggctgtgc cagctccgcc gcgagctgga
     5701 tgacctggaa cagtggatcc aggagcgcga ggtggtggcg gcctcccacg agctgggcca
     5761 ggactacgag catgtgacta tgctccgaga caaattccga gagttctccc gggacacaag
     5821 caccatcggt caggagcgcg tagatagcgc caatgcgctg gccaatgggc tcattgctgg
     5881 gggccatgct gcacgggcca ccgtggccga gtggaaggac agtctcaacg aggcctgggc
     5941 tgacctgctt gagctgctgg acacacgggg tcaggtgctg gccgcggcgt acgagctgca
     6001 gcgcttcctg cacggggcac gccaagccct ggcgcgggtg cagcacaagc agcagcagct
     6061 tccggacggg actggccgcg acctcaacgc tgccgaggcc ctgcagcgcc gacactgtgc
     6121 ctacgagcat gacattcagg ccctcagccc ccaggtccag caggtgcagg acgacggcca
     6181 ccggctccag aaggcctacg ctggagacaa ggctgaggag atcggccgcc acatgcaggc
     6241 cgtggccgag gcctgggccc agcttcaggg aagctctgcc gcccgccggc agctgctgct
     6301 ggacaccaca gacaagttcc gcttcttcaa ggctgtccgg gaactgatgc tctggatgga
     6361 tgaggtcaac ctgcagatgg atgcccagga gcgtccccgg gatgtgtcct ccgcggatct
     6421 agtcatcaag aaccagcaag gcatcaaggc agagatagag gcccgggcag accgcttctc
     6481 ctcctgcatc gacatgggga aggagctgct ggccaggagc cactatgcgg ccgaggagat
     6541 ctcagagaag ctgtctcagc tgcaggcacg gcgccaggag acagctgaga agtggcagga
     6601 gaagatggac tggcttcagc tggttttgga ggtgcttgtg tttggaagag atgcagggat
     6661 ggcagaggcc tggctctgca gccaggagcc actggtgcgc agcgctgagc tgggttgcac
     6721 ggtcgacgaa gttgagagcc tcatcaagcg gcacgaggcc ttccagaagt cagcagtggc
     6781 ctgggaggag cgattctgtg cgctggagaa gcttactgcg ctagaggagc gggagaagga
     6841 gcgaaagaga aagagggagg aggaggagcg gcggaaacag ccgcctgctc ccgaacccac
     6901 agccagtgtg cctccagggg acctggtggg cggccagaca gcttctgaca ccacctggga
     6961 cggaacccag ccacggccac caccatccac acaagcaccc agtgttaatg gagtctgcac
     7021 agatggagag ccctcacagc ccctgctggg acaacagaga cttgagcaca gcagcttccc
     7081 cgaagggccg ggacctggct caggggacga agccaatggg ccccggggag agaggcagac
     7141 ccggactcgg ggcccggccc catctgcaat gccccagagc aggtctaccg agtcagccca
     7201 tgctgccacc ctgccgcctc gaggcccaga gccatctgcc caggagcaga tggaggggat
     7261 gctgtgccgc aagcaggaga tggaggcctt cgggaagaag gctgccaaca ggtcctggca
     7321 gaacgtgtac tgtgtcctgc ggcgtgggag cctcggcttt tacaaggatg ccaaggcagc
     7381 cagcgcggga gtgccatacc acggagaagt gcctgtcagc ctggccaggg cccagggcag
     7441 cgtcgccttt gattaccgaa agcgcaaaca tgtcttcaag ctgggcttac aggatggaaa
     7501 agaatattta ttccaggcca aggatgaggc agagatgagc tcgtggctac gggtggtgaa
     7561 tgcagccatt gccacagcgt cttctgcctc tggagagcct gaagagccgg tggtgcccag
     7621 caccacccgg ggcatgaccc gggccatgac catgccccca gtgtcacccg tcggggctga
     7681 ggggcctgtt gtgctccgca gcaaagacgg cagagaacga gagcgagaaa aacgcttcag
     7741 cttctttaag aagaacaagt agttgggggc aaggtcccag gccaactccc tccctccgtt
     7801 caggaaactg ccagggacag tcgacaggga ccgccctctt gtcaggacaa ctgcctgctg
     7861 ctagggtctg ttgccaaggt caacccatca ccaggaactg tcactgggga cgagtccatg
     7921 ttcccaaggg cagcccttct cttctgctgt ttaattccag actggtggtg ggacccaggt
     7981 aaccccctct cccacccccg ccgacttctc ccctttcccc agcctcgtgc ctctgtccct
     8041 caccacggtg tggacagtgc cgcaccctca acataggcca tgtggggagt ggctgcccct
     8101 gcctcagggt cattctcctg ccatgcgagg gcactcgcct tctgccttct ggttcctcac
     8161 ccctcagacc agccaggaac ctctcagagc tgaagcaggc cctgggggca gaagtgccag
     8221 atgacagtca gaggcgcagg agccctccct ccccaccccc accctgtaac tccagctgcc
     8281 actccatctc cagctgctct caatggcttc caggtgtgtt gttcggggac agccaccgcc
     8341 ttgagtctgg ccaaggaggt gattaaacag ctcagcttct ctcaccgcct ctgggtgtct
     8401 ttgctttcct gaccacagcc tctctgccca agcctgtgtc ctctccttcc gttctggtct
     8461 ccttcatctc acgtttcccc agcctccaag ctcttgaaga gccgtgtcac actgagagct
     8521 tccctgacct ggtctcattt ccctctgaca gtgacctcca gtgtgctggg ttgaccaatg
     8581 ccaagcctct ttttaaacca aagtagttca ccatgggttg ggatgatgtc aacaagcagt
     8641 attcatgtta aaaagcaatg agggactcct tccctggtgc acaattttct tcacttgtga
     8701 tctctaaact gttttcaaat tggaagactg ggaagcattg ctacagcccc agccagtctc
     8761 agccactgct cagcccagtg gtggaaagct gagcacacag cgggtgctta ctgcccccac
     8821 ttgccgtctg ctctccagta gatcaaggga cctcagacag ggatctgaga agtggcaggt
     8881 tccaataaac tgatcaacag aatgaagtca ctctgggaag ccatgaggtg actttacagc
     8941 ccaagcatgt aaatatacac acagaggata atatatattc tttttttttt ttttttttga
     9001 gacagagtct cactctgtca gccaagctgg actgcagtgg cgcaatcaca gcccattgca
     9061 acctccacct cctgggttca agcaatcctc ccacctcagc ctcccgagta gcctgaacca
     9121 caggcacatg ccatcatacc cgactaagtc tgtttgtttt ttgtagagac ggggtctcac
     9181 tatgttgccc aggtcggtct cgaattcctg ggcccaagtg attctctcac cttggcctca
     9241 aactgttggg attacaggcg tgagccacca tgcccagctg agactacctt ctggatctga
     9301 tgaagtatta gaaaggagcg ggtggcttct cctttgtctc tggtttggca atcggagctt
     9361 tcagggcaca gtgtttgtct ccagatgttg atggaagtgg gcactccttg gtggcctcca
     9421 acaggaaaag cgtgttcttt cattgtttcc tatacacaca gacaaccgtt tttccaggaa
     9481 ggcagcccca tctttagtca ttgactgcgg gcctatcatc agtcatgacg aagaccgagt
     9541 taggtgtgtg caggcccaga actagcacat cacagcccca gagtgggctc caaaccggta
     9601 gaccagagag cctgcaggat aatttccccc ttaagatggg tagatttact gttccacagc
     9661 caacaggtgg atacgcgtaa ctagaacaat ctgtcatggg actaagtgga agaatccatc
     9721 agcactacac agcatctttt cttcaaatac aactgtaaaa tctccactga agagacagtc
     9781 ataccttggg ggtgctcagg gaatctaggt atttgcactc caagctggca agcctggggg
     9841 cactgaaggg aaacaataat tggaaatgtc agaaggaaat gtatatggag aaattaatag
     9901 agaaatgatt attttaacac gacacgtaag tgtaaatagg acagccaatt gaagcagtcc
     9961 tgcagtaggg gcaggcattg tcaacggaga ggtgagactg aagaccccga gcagcatctg
    10021 catatgcggc agcaggactt cagggcatga ttccttaaga acaggacata gaagagggga
    10081 agcatagtgt caggaattaa gtcagaaaac accttcagct gtgagtggaa gaaagaaaaa
    10141 attccaggaa agacaacagg atggaatggg gcggtgaggg ctatgagaca agttagacac
    10201 aaaaatacaa taaatgcagg cagcacaaat caatggagag agatggcaga taaaaatgga
    10261 ttacttgggc cgggcgcggt ggctcacgcc tgtaatccca gcactttggg aggccgaggc
    10321 gggcggatca cgaggtcagg agatcgagac catcccggct aaaacggtga aaccccgtct
    10381 ctactaaaaa tacaaaaatt agccgggcgt agtggcgggc gcctgtagtc ccagctactt
    10441 gggaggctga ggcaggagaa tggcgtgaac ccgggaggcg gagcttgcag tgagccgaga
    10501 tgccgccact gcgctccagc ctgggtgaca gagcgagact ccatctcaaa aaaaaaaaaa
    10561 aaaaaaaaat ggattacttg gccaggcatg gtggttcacg cttataatcc cagcactttg
    10621 ggaagccgag gtgagtgggt cacttgagct caggagttca agaccagcct gggcaacatg
    10681 gtgaaacccc atctctacaa aaattagcca ggtgtagtgg catgtgcctg tagtctcagc
    10741 tacttgggag gctgaggcag gagcattgct tgaacctggg aagcagaggt tacagtgagc
    10801 cgagatcatg ccactgcact ccagtctagg caataaagtg agaccctgtc tcaaaaaaaa
    10861 aaaaatagga ttatgtagag aaaaatatat ttgcattctt aacatcacct acaagagtgg
    10921 actttaggtc catgctgtcc acagcagcag ccacccagcc atgtgtggct actgagatgt
    10981 agccaaacca agtgtgtcat gactctgaaa caccaggtat gaagcatgga agaggacaac
    11041 aactttaaat accttaattt ttatattgat tatgtgttga aataatattt tagatatatt
    11101 gggtttcaaa agtatcatta aagtgaattt caacta
//
