LOCUS       NM_003128              10211 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript
            variant 1, mRNA.
ACCESSION   NM_003128
VERSION     NM_003128.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Yang,R., Ruan,B., Wang,R., Zhang,X., Xing,P., Li,C., Zhang,Y.,
            Chang,X., Song,H., Zhang,S., Zhao,H., Zhang,F., Yin,T., Qi,T.,
            Yan,W., Zhang,F., Hu,G., Wang,S. and Tao,L.
  TITLE     Cardiomyocyte betaII spectrin plays a critical role in maintaining
            cardiac function by regulating mitochondrial respiratory function
  JOURNAL   Cardiovasc Res 120 (11), 1312-1326 (2024)
   PUBMED   38832923
  REMARK    GeneRIF: Cardiomyocyte betaII spectrin plays a critical role in
            maintaining cardiac function by regulating mitochondrial
            respiratory function.
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   Jung,J. and Wu,Q.
  TITLE     Identification of bone mineral density associated genes with shared
            genetic architectures across multiple tissues: Functional insights
            for EPDR1, PKDCC, and SPTBN1
  JOURNAL   PLoS One 19 (4), e0300535 (2024)
   PUBMED   38683846
  REMARK    GeneRIF: Identification of bone mineral density associated genes
            with shared genetic architectures across multiple tissues:
            Functional insights for EPDR1, PKDCC, and SPTBN1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10211)
  AUTHORS   Sert,O., Ding,X., Zhang,C., Mi,R., Hoke,A. and Rasband,M.N.
  TITLE     Postsynaptic beta1 spectrin maintains Na+ channels at the
            neuromuscular junction
  JOURNAL   J Physiol 602 (6), 1127-1145 (2024)
   PUBMED   38441922
  REMARK    GeneRIF: Postsynaptic beta1 spectrin maintains Na[+] channels at
            the neuromuscular junction.
REFERENCE   4  (bases 1 to 10211)
  AUTHORS   Wu,H., Jiang,N., Li,J., Jin,Q., Jin,J., Guo,J., Wei,X., Wang,X.,
            Yao,L., Meng,D. and Zhi,X.
  TITLE     Tumor cell SPTBN1 inhibits M2 polarization of macrophages by
            suppressing CXCL1 expression
  JOURNAL   J Cell Physiol 239 (1), 97-111 (2024)
   PUBMED   37921259
  REMARK    GeneRIF: Tumor cell SPTBN1 inhibits M2 polarization of macrophages
            by suppressing CXCL1 expression.
REFERENCE   5  (bases 1 to 10211)
  AUTHORS   Teveroni,E., Di Nicuolo,F., Vergani,E., Oliva,A., Vodola,E.P.,
            Bianchetti,G., Maulucci,G., De Spirito,M., Cenci,T., Pierconti,F.,
            Gulino,G., Iavarone,F., Urbani,A., Milardi,D., Pontecorvi,A. and
            Mancini,F.
  TITLE     SPTBN1 Mediates the Cytoplasmic Constraint of PTTG1, Impairing Its
            Oncogenic Activity in Human Seminoma
  JOURNAL   Int J Mol Sci 24 (23), 16891 (2023)
   PUBMED   38069214
  REMARK    GeneRIF: SPTBN1 Mediates the Cytoplasmic Constraint of PTTG1,
            Impairing Its Oncogenic Activity in Human Seminoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10211)
  AUTHORS   Shimizu,T., Takakuwa,Y., Koizumi,H., Ishibashi,T. and Ohkawara,A.
  TITLE     Calcium-dependent peripheral localization of 4.1-like proteins and
            fodrin in cultured human keratinocytes
  JOURNAL   Biol Cell 86 (1), 19-26 (1996)
   PUBMED   8688828
REFERENCE   7  (bases 1 to 10211)
  AUTHORS   Chang,J.G., Scarpa,A., Eddy,R.L., Byers,M.G., Harris,A.S.,
            Morrow,J.S., Watkins,P., Shows,T.B. and Forget,B.G.
  TITLE     Cloning of a portion of the chromosomal gene and cDNA for human
            beta-fodrin, the nonerythroid form of beta-spectrin
  JOURNAL   Genomics 17 (2), 287-293 (1993)
   PUBMED   8406479
REFERENCE   8  (bases 1 to 10211)
  AUTHORS   Chardin,P., Camonis,J.H., Gale,N.W., van Aelst,L., Schlessinger,J.,
            Wigler,M.H. and Bar-Sagi,D.
  TITLE     Human Sos1: a guanine nucleotide exchange factor for Ras that binds
            to GRB2
  JOURNAL   Science 260 (5112), 1338-1343 (1993)
   PUBMED   8493579
REFERENCE   9  (bases 1 to 10211)
  AUTHORS   Hu,R.J., Watanabe,M. and Bennett,V.
  TITLE     Characterization of human brain cDNA encoding the general isoform
            of beta-spectrin
  JOURNAL   J Biol Chem 267 (26), 18715-18722 (1992)
   PUBMED   1527002
REFERENCE   10 (bases 1 to 10211)
  AUTHORS   Yoon,S.H., Skalka,H. and Prchal,J.T.
  TITLE     Presence of erythroid and nonerythroid spectrin transcripts in
            human lens and cerebellum
  JOURNAL   Invest Ophthalmol Vis Sci 30 (8), 1860-1866 (1989)
   PUBMED   2474519
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP218428.1, BP312037.1,
            AB209748.1, AC093110.4 and BM679686.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003128.2.
            
            Summary: Spectrin is an actin crosslinking and molecular scaffold
            protein that links the plasma membrane to the actin cytoskeleton,
            and functions in the determination of cell shape, arrangement of
            transmembrane proteins, and organization of organelles. It is
            composed of two antiparallel dimers of alpha- and beta- subunits.
            This gene is one member of a family of beta-spectrin genes. The
            encoded protein contains an N-terminal actin-binding domain, and 17
            spectrin repeats which are involved in dimer formation. Multiple
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1), also known as long or
            sigma-1, represents the longer transcript and encodes the longer
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU794662.1, AB371586.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356805.9/ ENSP00000349259.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-95                BP218428.1         11-105
            96-112              BP312037.1         96-112
            113-850             AB209748.1         1-738
            851-851             AC093110.4         55683-55683
            852-1985            AB209748.1         740-1873
            1986-1986           AC093110.4         66229-66229
            1987-7360           AB209748.1         1875-7248
            7361-9567           AC093110.4         106626-108832
            9568-10211          BM679686.1         18-661              c
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p16.2"
     gene            1..10211
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /note="spectrin beta, non-erythrocytic 1"
                     /db_xref="GeneID:6711"
                     /db_xref="HGNC:HGNC:11275"
                     /db_xref="MIM:182790"
     exon            1..192
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            193..387
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             240..7334
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     beta-G spectrin; beta-spectrin 2; beta-spectrin II;
                     beta-fodrin; embryonic liver beta-fodrin; spectrin beta
                     chain, brain 1; spectrin beta chain, non-erythrocytic 1;
                     beta-spectrin non-erythrocytic 1; epididymis luminal
                     protein 102; beta-II spectrin; fodrin beta chain;
                     spectrin, non-erythroid beta chain 1"
                     /codon_start=1
                     /product="spectrin beta chain, non-erythrocytic 1 isoform
                     1"
                     /protein_id="NP_003119.2"
                     /db_xref="CCDS:CCDS33198.1"
                     /db_xref="GeneID:6711"
                     /db_xref="HGNC:HGNC:11275"
                     /db_xref="MIM:182790"
                     /translation="MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSR
                     IKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPK
                     PTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQ
                     DISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPD
                     LIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHY
                     FSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSL
                     VGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDI
                     NKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNF
                     GFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIR
                     LWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDL
                     LQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELC
                     QLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRA
                     FEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRL
                     EEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRP
                     TLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFS
                     EADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQ
                     LMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIR
                     EKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQA
                     QAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASE
                     DMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQA
                     LDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGA
                     EAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRE
                     TASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFM
                     AELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFD
                     ANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEI
                     EELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDV
                     EDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIV
                     TDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSE
                     QELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESER
                     ISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHEL
                     GQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLN
                     EAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETL
                     QRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDAC
                     ESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKA
                     EIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLI
                     LEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFS
                     ALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGL
                     PAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPA
                     RTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIP
                     YHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAI
                     SSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK
                     "
     exon            388..539
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            540..713
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            714..805
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            806..886
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1002
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1115
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1303
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1421
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1580
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1883
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2037
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2908
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2909..3046
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3047..3803
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3804..4006
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4007..4097
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4098..4244
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4245..4508
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4509..4733
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4734..4823
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4824..5105
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            5106..5236
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            5237..5441
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            5442..5816
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            5817..6061
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6062..6200
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6201..6285
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6286..6482
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6483..6595
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6596..6659
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6660..6898
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6899..7072
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            7073..7115
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            7116..10211
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8421..8426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /note="hexamer: ATTAAA"
     polyA_site      8440
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /note="major polyA site"
     regulatory      8607..8612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /note="hexamer: AATAAA"
     polyA_site      8633
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
     regulatory      9464..9469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /note="hexamer: AATAAA"
     polyA_site      9484
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
     regulatory      10190..10195
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
                     /note="hexamer: AATAAA"
     polyA_site      10211
                     /gene="SPTBN1"
                     /gene_synonym="betaSpII; DDISBA; ELF; HEL102; SPTB2"
ORIGIN      
        1 agtccctccc tcggccgcct ctcctcccgg agcgagcgcg cagccctgcg cagcagcgcc
       61 cactggtccc gtcctgtgag ccccggcccc agccgcggac agacccgcgg agtcgcctcc
      121 cggcccaccc gcccggccgc cgaggagcgg gaggaggacg ggaccccggc gcccccaccc
      181 catccccggg agaactctaa gaaggagctg atgtggagga gcagctgaga cagttcaaga
      241 tgacgaccac agtagccaca gactatgaca acattgagat ccagcagcag tacagtgatg
      301 tcaacaaccg ctgggatgtc gacgactggg acaatgagaa cagctctgcg cggctttttg
      361 agcggtcccg catcaaggct ctggcagatg agcgtgaagc cgtgcagaag aagaccttca
      421 ccaagtgggt caattcccac cttgcccgtg tgtcctgccg gatcacagac ctgtacactg
      481 accttcgaga tggacggatg ctcatcaagc tgctggaggt cctctctgga gagaggctgc
      541 ctaaacccac caagggacga atgcgcatcc actgcttaga gaatgtggac aaggcccttc
      601 agttcctgaa ggagcagaga gtccatcttg agaacatggg gtcccatgac atcgtggatg
      661 gaaaccaccg gctgaccctt ggcctcatct ggaccatcat cctgcgcttc cagatccagg
      721 atatcagtgt ggaaactgaa gacaacaaag agaagaaatc tgccaaggat gcattgctgt
      781 tgtggtgcca gatgaagaca gctgggtacc ccaatgtcaa cattcacaat ttcaccacta
      841 gctggaggga cggcatggcc ttcaatgcac tgatacacaa acaccggcct gacctgatag
      901 attttgacaa actaaagaaa tctaacgcac actacaacct gcagaatgca tttaatctgg
      961 cagaacagca cctcggcctc actaaactgt tggaccccga agacatcagc gtggaccatc
     1021 ctgatgagaa gtccataatc acttatgtgg tgacttatta ccactacttc tctaagatga
     1081 aggccttagc tgttgaagga aaacgaattg gaaaggtgct tgacaatgct attgaaacag
     1141 aaaaaatgat tgaaaagtat gaatcacttg cctctgacct tctggaatgg attgaacaaa
     1201 ccatcatcat tctgaacaat cgcaaatttg ccaattcact ggtcggggtt caacagcagc
     1261 ttcaggcatt caacacttac cgcactgtgg agaaaccacc caaatttact gagaagggga
     1321 acttggaagt gctgctcttc accattcaga gcaagatgag ggccaacaac cagaaggtct
     1381 acatgccccg ggaggggaag ctcatctctg acatcaacaa ggcctgggaa agactggaaa
     1441 aagcggaaca cgaaagagaa ctggctttgc ggaatgagct cataagacag gagaaactgg
     1501 aacagctcgc ccgcagattt gatcgcaagg cagctatgag ggagacttgg ctgagcgaaa
     1561 accagcgtct ggtgtctcag gacaactttg ggtttgacct tcctgcagtt gaggccgcca
     1621 caaaaaagca cgaggccatt gagacagaca ttgccgcata cgaggagcgt gtgcaggctg
     1681 tggtagccgt ggccagggag ctcgaggccg agaattacca cgacatcaag cgcatcacag
     1741 cgaggaagga caatgtcatc cggctctggg aatacctact ggaactgctc agggcccgga
     1801 gacagcggct cgagatgaac ctggggctgc agaagatatt ccaggaaatg ctctacatta
     1861 tggactggat ggatgaaatg aaggtgctag tattgtctca agactatggc aaacacttac
     1921 ttggtgtgga agacctgtta cagaagcaca ccctggttga agcagacatt ggcatccagg
     1981 cagagcgggt gagaggtgtc aatgcctccg cccagaagtt cgcaacagac ggggaaggtt
     2041 acaagccctg tgacccccag gtgatccgag accgcgtggc ccacatggag ttctgttatc
     2101 aagagctttg ccagctggcg gctgagcgca gggcccgtct ggaagagtcc cgccgcctct
     2161 ggaagttctt ctgggagatg gcagaagagg aaggctggat acgggagaag gagaagatcc
     2221 tgtcctcgga cgattacggg aaagacctga ccagcgtcat gcgcctgctc agcaagcacc
     2281 gggcgttcga ggacgagatg agcggccgca gtggccactt tgagcaggcc atcaaggaag
     2341 gcgaagacat gatcgcggag gagcacttcg ggtcggagaa gatccgtgag aggatcattt
     2401 acatccggga gcagtgggcc aacctagagc agctctcggc cattcggaag aagcgcctgg
     2461 aggaggcctc cctgctgcac cagttccagg cagatgctga tgacattgat gcctggatgc
     2521 tggacatcct caagattgtc tccagcagcg acgtgggcca cgatgagtat tccacacagt
     2581 ctctggtcaa gaaacacaag gacgtggcgg aagagatcgc caattacagg cccacccttg
     2641 acacgctgca cgaacaagcc agcgccctcc cccaggagca tgccgagtct ccagacgtga
     2701 ggggcaggct gtcgggcatc gaggagcggt ataaggaggt ggcagagctg acgcggctgc
     2761 ggaagcaggc actccaggac actctggccc tgtacaagat gttcagcgag gctgatgcct
     2821 gtgagctctg gatcgacgag aaggagcagt ggctcaacaa catgcagatc ccagagaagc
     2881 tggaggatct ggaggtcatc cagcacagat ttgagagcct agaaccagaa atgaacaacc
     2941 aggcttcccg ggttgcagtg gtgaaccaga ttgcacgcca gctgatgcac agcggccacc
     3001 caagtgagaa ggaaatcaaa gcccagcagg acaaactcaa cacaaggtgg agccagttca
     3061 gagaactggt tgacaggaag aaggatgccc tcctgtctgc cctgagcatc cagaactacc
     3121 acctcgagtg caatgaaacc aaatcctgga ttcgggaaaa gaccaaggtc atcgagtcca
     3181 cccaggacct gggcaatgac ctggctggcg tcatggccct gcagcgcaag ctgaccggca
     3241 tggagcggga cttggtggcc attgaggcaa agctgagtga cctgcagaag gaggcggaga
     3301 agctggagtc cgagcacccc gaccaggccc aggccatcct gtctcggctg gccgagatca
     3361 gcgacgtgtg ggaggagatg aagaccaccc tgaaaaaccg agaggcctcc ctgggagagg
     3421 ccagcaagct gcagcagttc ctacgggact tggacgactt ccagtcctgg ctctctagga
     3481 cccagacagc gatcgcctcg gaggacatgc caaacaccct gaccgaggct gagaagctgc
     3541 tcacgcagca cgagaacatc aagaacgaga tcgacaacta cgaggaggac taccagaaga
     3601 tgagggacat gggcgagatg gtcacccagg ggcagaccga tgcccagtac atgtttctgc
     3661 ggcagcggct gcaggccctg gacactggat ggaacgagct ccacaagatg tgggagaaca
     3721 gacaaaatct cctatcccag tcacatgcct accagcagtt cctcagagac acgaagcaag
     3781 ccgaagcctt tcttaacaac caggagtatg ttctggctca cactgaaatg cctaccacct
     3841 tggaaggagc tgaagcagca attaaaaagc aagaggactt catgaccacc atggacgcca
     3901 atgaggagaa gatcaatgct gtggtggaga ctggccggag gctggtgagc gatgggaaca
     3961 tcaactcaga tcgcatccag gagaaggtgg actctattga tgacagacat aggaagaatc
     4021 gtgagacagc cagtgaactt ttgatgaggt tgaaggacaa cagggatcta cagaaattcc
     4081 tgcaagattg tcaagagctg tctctctgga tcaatgagaa gatgctcaca gcccaggaca
     4141 tgtcttacga tgaagccaga aatctgcaca gtaaatggtt gaagcatcaa gcatttatgg
     4201 cagaacttgc atccaacaaa gaatggcttg acaaaatcga gaaggaagga atgcagctca
     4261 tttcagaaaa gcctgagacg gaagctgtgg tgaaggagaa actcactggt ttacataaaa
     4321 tgtgggaagt ccttgaatcc actacccaga caaaggccca gcggctcttt gatgcaaaca
     4381 aggccgaact tttcacccag agctgtgcag atctagacaa atggctgcac ggcctggaga
     4441 gtcagattca gtctgatgac tatggcaaag acctgaccag tgtcaatatc ctgctgaaaa
     4501 agcaacagat gctggagaat cagatggaag tgcggaagaa ggagatcgaa gagctccaaa
     4561 gccaagccca ggccctgagt caggaaggga agagcaccga cgaggtagac agcaagcgcc
     4621 tcaccgtgca gaccaagttc atggagttgc tggagccctt gaacgagagg aagcataacc
     4681 tgctggcctc caaagagatc catcagttca acagggatgt ggaggacgag atcttgtggg
     4741 ttggagagag gatgcctttg gcaacttcca cggatcatgg ccacaacctc cagactgtgc
     4801 agctgttaat aaagaaaaat cagaccctcc agaaagaaat ccaggggcac cagcctcgca
     4861 ttgacgacat ctttgagagg agccaaaaca tcgtcactga cagcagcagc ctcagcgctg
     4921 aggccatcag acagaggctt gccgacctga agcagctgtg gggtctcctc attgaggaga
     4981 cagagaaacg ccacaggcgg ctggaggagg cgcacagggc ccagcagtac tactttgacg
     5041 ctgctgaggc cgaagcctgg atgagcgagc aggagctgta catgatgtca gaggagaagg
     5101 ccaaggatga gcagagtgct gtctccatgt tgaagaagca ccagatctta gaacaagctg
     5161 tggaggacta tgcagagacc gtgcatcagc tctccaagac cagccgggcc ctggtggccg
     5221 acagccatcc tgaaagtgag cgcattagca tgcggcagtc caaagtggat aaactgtacg
     5281 ctggtctgaa agaccttgct gaagagagaa gaggcaagct ggatgagaga cacaggttat
     5341 tccagctcaa ccgggaggtg gacgacctgg agcagtggat cgctgagagg gaggtggtcg
     5401 cagggtccca tgaactggga caggactatg agcatgtcac gatgttacaa gaacgattcc
     5461 gggagtttgc ccgagacacc gggaacattg ggcaggagcg cgtggacacg gtcaatcacc
     5521 tggcagatga gctcatcaac tctggacatt cagatgccgc caccatcgct gaatggaagg
     5581 atggcctcaa tgaagcctgg gccgacctcc tggagctcat tgacacaaga acacagattc
     5641 ttgccgcttc ctatgaactg cacaagtttt accacgatgc caaggagatc tttgggcgta
     5701 tacaggacaa acacaagaaa ctccctgagg agcttgggag agatcagaac acagtggaga
     5761 ccttacagag aatgcacact acatttgagc atgacatcca ggctctgggc acacaggtga
     5821 ggcagctgca ggaggatgca gcccgcctcc aggcggccta tgcgggtgac aaggccgacg
     5881 atatccagaa gcgcgagaac gaggtcctgg aagcctggaa gtccctcctg gacgcctgtg
     5941 agagccgcag ggtgcggctg gtggacacag gggacaagtt ccgcttcttc agcatggtgc
     6001 gcgacctcat gctctggatg gaggatgtca tccggcagat cgaggcccag gagaagccaa
     6061 gggatgtatc atctgttgaa ctcttaatga ataatcatca aggcatcaaa gctgaaattg
     6121 atgcacgtaa tgacagtttc acaacctgca ttgaacttgg gaaatccctg ttggcgagaa
     6181 aacactatgc atctgaggag atcaaggaaa aattactgca gttgacggaa aagaggaaag
     6241 aaatgatcga caagtgggaa gaccgatggg aatggttaag actgattctg gaggtccatc
     6301 agttctcaag agacgccagt gtggccgagg cctggctgct tggacaggag ccgtacctat
     6361 ccagccgaga gataggccag agcgtggacg aggtggagaa gctcatcaag cgccacgagg
     6421 catttgaaaa gtctgcagca acctgggatg agaggttctc tgccctggaa aggctgacta
     6481 cattggagtt actggaagtg cgcagacagc aagaggaaga ggagaggaag aggcggccgc
     6541 cttctcccga gccgagcacg aaggtttcag aggaagccga gtcccagcag cagtgggata
     6601 cttcaaaagg agaacaagtt tcccaaaacg gtttgccagc tgaacaggga tctccacgga
     6661 tggcagaaac ggtggacaca agcgaaatgg tcaacggcgc tacagaacaa aggacgagct
     6721 ctaaagagtc cagccccatc ccctccccga cctctgatcg taaagccaag actgccctcc
     6781 cagcccagag tgccgccacc ttaccagcca gaacccagga gacaccttcg gcccagatgg
     6841 aaggcttcct caatcggaaa cacgagtggg aggcccacaa taagaaagcc tcaagcaggt
     6901 cctggcacaa tgtttattgt gtcataaata accaagaaat gggtttctac aaagatgcaa
     6961 agactgctgc ttctggaatt ccctaccaca gcgaggtccc tgtgagtttg aaagaagctg
     7021 tctgcgaagt ggcccttgat tacaaaaaga agaaacacgt attcaagcta agactaaatg
     7081 atggcaatga gtacctcttc caagccaaag acgatgagga aatgaacaca tggatccagg
     7141 ctatctcttc cgccatctcc tctgataaac acgaggtgtc tgccagcacc cagagcacgc
     7201 cagcatccag ccgcgcgcag accctcccca ccagcgtcgt caccatcacc agcgagtcca
     7261 gtcccggcaa gcgggaaaag gacaaagaga aagacaaaga gaagcggttc agcctttttg
     7321 gcaaaaagaa atgaactcct ttccttcacc tcctgccctt ctcttacctt ttcagtgaaa
     7381 ttccagcatg caagctcaga accaacacat tactctctgt gcctaatgtt cctcaatgtg
     7441 gttgattttt tttttttttt aatttataga gcatttcggg gggggtgggg gaaacacacc
     7501 taaacacttt atctccaagt tacaaaagtt tgaggtgcag agggaaggcc agattttttt
     7561 tttaatgaaa ttatatagat tagatctcag tatttaaact gttcctcaat tttgtgaggc
     7621 tgtgttggaa ataacccgcc tctagtgctg ttggtatgca aggcagcggt gcttaatcaa
     7681 tatttcctgt gctcaccaga ggcaaaatgt accaatatcc tgacaccatt ctctctccat
     7741 ttacttctgg tggttaccct gactcttgac tcttagaagt gcccgagatg gggctaacct
     7801 ttattaaaca gatcgcatat tatgatcttg ctgcagccac agtgcagctc cacattaact
     7861 ctacagacca aaccatttgt atctggcatc acttactaac acacgacatg cggcttttct
     7921 gcatcaactg ctatgacggt taagaatgtc agtatacaag aaggaataga aaactgatac
     7981 tgttttaaat aatctgtaat ttcaattttt tttttttgct gaaatacatt atattgtacg
     8041 tttgagataa ttctagtaca aagtataata aaactagatg tataataaac cctttaaatc
     8101 attggtaagt gtacaagtgg tggaactgaa gcatttactg gacaaagtaa tgttactcta
     8161 atggttactt gctcgtgcgt tgccacactg tgttataatt tgcttcattt ccttgctatt
     8221 tgatacatag tgtgcatttc tctgtcactg taactattgt aatgacaaat tttcatctta
     8281 ctgcacaatc aaaatgacat tgataggaat gaactccaga ggctgggcct gaacagggag
     8341 gtggtcgctc aggcctggtg ctcagtcgta cgacctgtac ctctcaactt ttgccctatc
     8401 tgttaaatat atgctatgtc attaaatgct tttaaatcta gcacggtgac tagttgttgt
     8461 tcttcctctg ctgcgtgtgc atgcccagta gggaaactgc aaagggagaa atgacaaaca
     8521 agaaacattt tacaaccagt ctgggctcac ttttgcattt tttatgcatg tctggtgcac
     8581 aagctttgaa aactacagca aacagtaata aatgtgactg ttttgtagtt atacattcag
     8641 gctttatctt ttatttatga aaaagttacc agagcacttg tgaaacctga actctgagat
     8701 aggggttggc aaatttgttc tgtaaaggga cagagatagt aaatatttta ggctttgtag
     8761 gccgtaactg tctctgttgc tcaattctgc tgttgcgtaa tacaaaggca gccattgacg
     8821 gtatgtgaat gcctgggcat ggctgtgttc cagtaaaact atttaaggac ataaatttga
     8881 atttcatata actttgacat gccatacaat attttgattt tcttaatgat ttcaaaatgt
     8941 aaagtctatt ttatacagat cagaccaaaa agaagaaaaa aaaaaaacag gcaagaggtt
     9001 aggtttggcc catgggccat agtttgctga cttcagcata gagtcgtggg ttatttacaa
     9061 gtgacatact tttcctgggt tatctgggta tgttgactcc atgccacttg cataaagcag
     9121 caatggatat tagtattatg gatgtccagt aagttattcc acaaagacca tgcctaaggt
     9181 ggcatcagcc ctgggctcca cagctgcgtg gcatcaaagc tttctcttaa ctctcttacc
     9241 tctaggcaaa ctgagacctc accatcctct cccctgcttc ccacgacagt cctttgccct
     9301 tgccatgctc agggttggaa tcaagttgtt ctattctcaa cagaccaaaa tgtttagtta
     9361 aggcaaagta tcttggaaac aattgtgatt aattacagtc ttgtactctt gacaaagctg
     9421 tgcagatggc aataagttca taccagcaat cctggagtcc cataataaat acgtacatgt
     9481 ggaacatcgt gcacataatt tcaacagttc gcagatctgt agttatgaag ccagggtttg
     9541 gtggtatttg ctctctcttg gtgcatttga taaggatcca ctgaggcctg aatgtggaag
     9601 atgatgggca gcgagaggag cgtcagaaga ccccagtcaa gacgtgttcg ccatcagagg
     9661 ttacaggccg cacagcctga tgagcttcaa gcctggcagg gtaaatagtt tttgggtttt
     9721 ttgttttttt tttattcttc cactatcatg ttttttgagg attttgcata tttcttgttg
     9781 ccataatgct gtgctatttt accctgattt tcagtgatac aatatgggtg acaatactgg
     9841 cccctccata aatctgaaga gtaagaagta gtgaaaataa ggcttaggat atgaaatggc
     9901 gttgtcactt gaatcaaggc cactttttgt cctacttgag catctcaagt tgggatgcat
     9961 cttctgatgg cacttccgga actggctgtg gtttttttgg gtgtaccgag agtgccagtg
    10021 actgtgcttc ttacaattcc tggcatcttg agtaggtgaa acactgtatc agactgggtg
    10081 atgggcacat tgtcatttca ccaagttcct ggaactgtta gaattgcttg tgtatgggga
    10141 tcctatgtta gttcccctgg atacattgtt ttatcagtcg gaattcttaa ataaagacat
    10201 acttcccttc a
//
