LOCUS       NM_001355436           10177 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens spectrin beta, erythrocytic (SPTB), transcript variant
            1, mRNA.
ACCESSION   NM_001355436
VERSION     NM_001355436.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10177)
  AUTHORS   Ge,Y., Li,J., Han,Y., Xie,H., Shangguan,S., Jiang,Q., Chen,X. and
            Liu,R.
  TITLE     [Analysis of the characteristics of SPTB gene variants among 16
            children with Hereditary spherocytosis]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 40 (3), 269-275 (2023)
   PUBMED   36854399
  REMARK    GeneRIF: [Analysis of the characteristics of SPTB gene variants
            among 16 children with Hereditary spherocytosis].
REFERENCE   2  (bases 1 to 10177)
  AUTHORS   Chen,X.L., Li,J.G., Men,Q. and Li,X.
  TITLE     [Genetic Analysis of a Chinese Pedigree with Hereditary
            Spherocytosis Caused by Copy Number Variation Deletion of SPTB
            Gene]
  JOURNAL   Zhongguo Shi Yan Xue Ye Xue Za Zhi 31 (1), 183-188 (2023)
   PUBMED   36765497
  REMARK    GeneRIF: [Genetic Analysis of a Chinese Pedigree with Hereditary
            Spherocytosis Caused by Copy Number Variation Deletion of SPTB
            Gene].
REFERENCE   3  (bases 1 to 10177)
  AUTHORS   Gallagher,P.G., Sabatino,D.E., Romana,M., Cline,A.P., Garrett,L.J.,
            Bodine,D.M. and Forget,B.G.
  TITLE     A human beta-spectrin gene promoter directs high level expression
            in erythroid but not muscle or neural cells
  JOURNAL   J Biol Chem 274 (10), 6062-6073 (1999)
   PUBMED   10037687
REFERENCE   4  (bases 1 to 10177)
  AUTHORS   Kanzaki,A., Rabodonirina,M., Yawata,Y., Wilmotte,R., Wada,H.,
            Ata,K., Yamada,O., Akatsuka,J., Iyori,H., Horiguchi,M. et al.
  TITLE     A deletional frameshift mutation of the beta-spectrin gene
            associated with elliptocytosis in spectrin Tokyo (beta 220/216)
  JOURNAL   Blood 80 (8), 2115-2121 (1992)
   PUBMED   1391962
REFERENCE   5  (bases 1 to 10177)
  AUTHORS   Winkelmann,J.C., Costa,F.F., Linzie,B.L. and Forget,B.G.
  TITLE     Beta spectrin in human skeletal muscle. Tissue-specific
            differential processing of 3' beta spectrin pre-mRNA generates a
            beta spectrin isoform with a unique carboxyl terminus
  JOURNAL   J Biol Chem 265 (33), 20449-20454 (1990)
   PUBMED   2243099
REFERENCE   6  (bases 1 to 10177)
  AUTHORS   Winkelmann,J.C., Chang,J.G., Tse,W.T., Scarpa,A.L., Marchesi,V.T.
            and Forget,B.G.
  TITLE     Full-length sequence of the cDNA for human erythroid beta-spectrin
  JOURNAL   J Biol Chem 265 (20), 11827-11832 (1990)
   PUBMED   2195026
REFERENCE   7  (bases 1 to 10177)
  AUTHORS   Fukushima,Y., Byers,M.G., Watkins,P.C., Winkelmann,J.C.,
            Forget,B.G. and Shows,T.B.
  TITLE     Assignment of the gene for beta-spectrin (SPTB) to chromosome
            14q23----q24.2 by in situ hybridization
  JOURNAL   Cytogenet Cell Genet 53 (4), 232-233 (1990)
   PUBMED   2209094
REFERENCE   8  (bases 1 to 10177)
  AUTHORS   Prchal,J.T., Morley,B.J., Yoon,S.H., Coetzer,T.L., Palek,J.,
            Conboy,J.G. and Kan,Y.W.
  TITLE     Isolation and characterization of cDNA clones for human erythrocyte
            beta-spectrin
  JOURNAL   Proc Natl Acad Sci U S A 84 (21), 7468-7472 (1987)
   PUBMED   3478706
REFERENCE   9  (bases 1 to 10177)
  AUTHORS   Goodman,S.R., Shiffer,K.A., Casoria,L.A. and Eyster,M.E.
  TITLE     Identification of the molecular defect in the erythrocyte membrane
            skeleton of some kindreds with hereditary spherocytosis
  JOURNAL   Blood 60 (3), 772-784 (1982)
   PUBMED   7104494
REFERENCE   10 (bases 1 to 10177)
  AUTHORS   Bennett,V. and Stenbuck,P.J.
  TITLE     The membrane attachment protein for spectrin is associated with
            band 3 in human erythrocyte membranes
  JOURNAL   Nature 280 (5722), 468-473 (1979)
   PUBMED   379653
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL135745.5 and AL121774.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 11, 2018 this sequence version replaced NM_001355436.1.
            
            Summary: This locus encodes a member of the spectrin gene family.
            Spectrin proteins, along with ankyrin, play a role in cell membrane
            organization and stability. The protein encoded by this locus
            functions in stability of erythrocyte membranes, and mutations in
            this gene have been associated with spherocytosis type 2,
            hereditary elliptocytosis, and neonatal hemolytic anemia.
            Alternatively spliced transcript variants have been described.
            [provided by RefSeq, Nov 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB209415.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000644917.1/ ENSP00000495909.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-116               AL135745.5         38609-38724
            117-315             AL121774.5         10821-11019
            316-467             AL121774.5         28876-29027
            468-641             AL121774.5         30186-30359
            642-733             AL121774.5         31649-31740
            734-814             AL121774.5         32132-32212
            815-930             AL121774.5         32566-32681
            931-1043            AL121774.5         33098-33210
            1044-1231           AL121774.5         34032-34219
            1232-1349           AL121774.5         36120-36237
            1350-1508           AL121774.5         37251-37409
            1509-1811           AL121774.5         38327-38629
            1812-1962           AL121774.5         39349-39499
            1963-2833           AL121774.5         40099-40969
            2834-2971           AL121774.5         42110-42247
            2972-3728           AL121774.5         46806-47562
            3729-3931           AL121774.5         48015-48217
            3932-4022           AL121774.5         48339-48429
            4023-4169           AL121774.5         49573-49719
            4170-4433           AL121774.5         51413-51676
            4434-4640           AL121774.5         54035-54241
            4641-4730           AL121774.5         54720-54809
            4731-5009           AL121774.5         58563-58841
            5010-5140           AL121774.5         59439-59569
            5141-5345           AL121774.5         60542-60746
            5346-5720           AL121774.5         61012-61386
            5721-5965           AL121774.5         62837-63081
            5966-6104           AL121774.5         64238-64376
            6105-6189           AL121774.5         64848-64932
            6190-6386           AL121774.5         66107-66303
            6387-6436           AL121774.5         66614-66663
            6437-6512           AL121774.5         67165-67240
            6513-6769           AL121774.5         80173-80429
            6770-6943           AL121774.5         83812-83985
            6944-6986           AL121774.5         84270-84312
            6987-10177          AL121774.5         84493-87683
FEATURES             Location/Qualifiers
     source          1..10177
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.3"
     gene            1..10177
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /note="spectrin beta, erythrocytic"
                     /db_xref="GeneID:6710"
                     /db_xref="HGNC:HGNC:11274"
                     /db_xref="MIM:182870"
     exon            1..116
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            117..315
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..7154
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /note="isoform a is encoded by transcript variant 1;
                     spectrin beta chain, erythrocyte; spectrin beta chain,
                     erythrocytic; Sp beta; spectrin beta Tandil; membrane
                     cytoskeletal protein; beta-I spectrin; erythrocytic
                     spectrin beta chain"
                     /codon_start=1
                     /product="spectrin beta chain, erythrocytic isoform a"
                     /protein_id="NP_001342365.1"
                     /db_xref="CCDS:CCDS32099.1"
                     /db_xref="GeneID:6710"
                     /db_xref="HGNC:HGNC:11274"
                     /db_xref="MIM:182870"
                     /translation="MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSR
                     IKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPK
                     PTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQ
                     DIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPD
                     LIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHY
                     FSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSL
                     TGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDI
                     NRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNF
                     GYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILR
                     LWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDL
                     LQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSN
                     MAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAF
                     EDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQ
                     DAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGV
                     MEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGE
                     TDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSL
                     VESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITD
                     KTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKE
                     DIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASED
                     MPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGL
                     DTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAE
                     AGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEK
                     AQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVA
                     ELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAA
                     RSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELG
                     ELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWV
                     EERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDC
                     QDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISD
                     EIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQV
                     DKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVT
                     LLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLE
                     LIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFE
                     RELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLV
                     DTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKN
                     FSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSR
                     DASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTL
                     ELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPGEEEGTWPQNLQQPPPP
                     GQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLPAPRDHGQSVQMEGYLGRKHD
                     LEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRHAICEIAAN
                     YKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLS
                     GPDASLGKKDKEKRFSFFPKKK"
     exon            316..467
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            468..641
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            642..733
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            734..814
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            815..930
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1043
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1231
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1349
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1508
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1811
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1962
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2833
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2834..2971
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2972..3728
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3729..3931
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3932..4022
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4023..4169
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4170..4433
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4434..4640
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4641..4730
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4731..5009
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5010..5140
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5141..5345
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5346..5720
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5721..5965
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5966..6104
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6105..6189
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6190..6386
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6387..6436
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6437..6512
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6513..6769
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6770..6943
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6944..6986
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6987..10177
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10158..10163
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /note="hexamer: AATAAA"
     polyA_site      10177
                     /gene="SPTB"
                     /gene_synonym="EL3; HS2; HSPTB1; SPH2"
                     /note="major polyA site"
ORIGIN      
        1 agcaccaggc gcccggcggc cgctgtgcca ggagccccgc gctgccctcc tccccctgcg
       61 ccgccgccgc cgccaccccc gggctcgggg tggcccccgc ttcagtccca gggcagggat
      121 ccttccatga agactgaggc aggcggagct gctaagagcc tgctgacatg acatcggcca
      181 cagagtttga aaatgtgggc aaccagccac cttacagcag gatcaatgcc cgctgggacg
      241 ccccagacga cgagctggat aatgacaaca gctcagccag gctctttgag aggtcccgga
      301 taaaggcctt ggcagatgag cgggaagttg ttcagaaaaa gaccttcacg aaatgggtga
      361 actcgcacct ggctcgagtg tcctgccgca tcaccgatct ctacaaggac ctgcgggatg
      421 ggcgcatgct catcaagctg ctggaggtgc tctctggaga gatgctgcca aagcccacca
      481 aggggaagat gcgcatccac tgcctggaga atgtggacaa ggctctccag ttcctcaagg
      541 agcagcgtgt acacctggag aacatgggct cccacgacat tgtagatggc aaccaccgcc
      601 tggtcctggg cctcatctgg accatcatcc tccgcttcca gattcaggac attgtggtcc
      661 aaactcagga aggtcgtgaa acacgctcag ccaaggatgc gttgctgttg tggtgtcaga
      721 tgaagacggc aggctaccct catgttaatg tcaccaactt tacctccagc tggaaggatg
      781 gcttggcctt taatgccctg atacacaagc accggcccga cctgatcgac tttgataagc
      841 tgaaggactc caatgcccgg cacaacctgg agcacgcatt caatgtggct gagcgccagc
      901 tgggcatcat cccgctcctc gaccccgaag atgtctttac ggaaaaccct gatgagaaat
      961 ccatcatcac ctatgtggtg gccttttacc actacttctc caagatgaag gtgctggcag
     1021 tggagggcaa gcgtgtcggc aaggttattg accatgccat tgagactgag aagatgattg
     1081 aaaagtacag cgggctagcc tcggacctgc tcacctggat cgagcagacc atcactgtcc
     1141 tgaacagccg caagtttgcc aactcgctga cgggcgtcca gcagcagctg caggccttca
     1201 gcacctaccg caccgtggag aagccgccca agtttcaaga gaaggggaat ctggaagttc
     1261 tactttttac catccagtcc cggatgagag ccaacaatca gaaagtgtac acaccccacg
     1321 atgggaaact agtgtctgac atcaacaggg cctgggaaag cctggaggaa gctgagtatc
     1381 ggcgggagct ggccctgaga aatgagctca ttcggcagga gaagctagag caactggccc
     1441 ggcgctttga ccggaaggcc gcaatgagag agacctggct cagtgaaaac cagcgcctcg
     1501 tggcccagga taactttggg tatgacctgg cagctgtgga ggccgccaag aagaagcatg
     1561 aggccatcga gaccgacacg gctgcctacg aggagcgggt gagagccctg gaggacctgg
     1621 ctcaggagct ggagaaagag aactaccatg accagaagcg catcacggcc cgcaaggaca
     1681 atatactgcg cctatggagc tacctgcagg agctgctgca gtcccggcgc cagaggctcg
     1741 agaccaccct ggcactgcag aagctcttcc aggacatgct gcacagcatc gactggatgg
     1801 atgagatcaa ggctcacctc ttgtctgccg agtttgggaa gcacttgttg gaggttgaag
     1861 acctgctaca gaagcacaag ttgatggaag ctgacatcgc catccaaggg gacaaagtga
     1921 aggccatcac cgcagccacc ctgaagttca ccgaggggaa agggtaccag ccttgtgacc
     1981 cccaggtcat ccaggaccgc atcagccact tggagcagtg ctttgaggag ctgagcaaca
     2041 tggcagctgg gcggaaggcc caactggagc agtccaaacg actctggaag ttcttctggg
     2101 agatggatga ggctgagagc tggatcaagg agaaggagca gatctattct tccctggact
     2161 atggcaaaga cctgaccagt gtgctcatct tacagcgcaa gcacaaggcc tttgaggatg
     2221 agctccgtgg gctggatgct cacctggagc agatcttcca ggaggctcat ggcatggttg
     2281 cgcgcaagca gtttgggcac ccgcagatcg aggcccgcat aaaggaggtg tcggcacagt
     2341 gggaccagct gaaggacctg gctgccttct gcaagaagaa cctccaggat gctgagaact
     2401 ttttccagtt ccagggcgat gcggatgacc tgaaggcttg gctgcaagac gcccaccggc
     2461 tgctctctgg tgaagatgtg gggcaggacg aaggggccac gcgggccctg gggaaaaagc
     2521 acaaggactt cctggaggag ctggaggaga gccgtggggt gatggagcac ctggagcagc
     2581 aggcccaggg attccccgaa gagtttcggg attccccaga tgtgacccat cggctgcagg
     2641 ccctgcggga gctctaccaa caggtggtgg cccaggcgga cctgcgtcag cagaggctgc
     2701 aggaagccct ggacctgtac acggtgttcg gggagacaga cgcctgtgag ctgtggatgg
     2761 gagagaagga gaagtggctg gccgagatgg aaatgccaga caccctggag gacctggagg
     2821 tcgtgcagca caggttcgac atcctggacc aggagatgaa gaccttgatg actcagattg
     2881 atggtgtgaa cctcgctgcc aacagcttgg tagagagtgg ccacccacgc agcagggagg
     2941 tgaagcagta ccaggaccat ctgaacacca ggtggcaggc atttcagacc ctggtgtcgg
     3001 agcggcggga ggctgtggac tcagccctcc gagtgcacaa ctactgcgta gattgcgagg
     3061 agaccagcaa gtggatcacg gacaagacaa aggtagtgga gtccacaaaa gacctggggc
     3121 gggacctggc aggtatcatc gccatccaga ggaagttgtc agggctggag cgtgacgtgg
     3181 ccgccatcca ggcccgtgtg gatgccctgg agcgtgagtc ccagcagctg atggactcgc
     3241 accctgagca gaaggaggat attggtcagc ggcaaaaaca cttggaggag ctgtggcagg
     3301 gcctgcagca atccctgcag ggccaggagg acttgctggg ggaagtcagc cagctgcagg
     3361 ccttcctgca ggatctggat gacttccagg cctggctctc catcacccag aaggctgtgg
     3421 cctctgagga catgcccgaa tccctcccag aggctgagca gctcctgcag cagcatgcag
     3481 gtatcaagga tgagattgac gggcaccaag acagctacca gcgtgttaag gagtctgggg
     3541 agaaagtgat ccaaggccag acggacccag agtatctgct tctgggccag cggctggagg
     3601 gcctggatac tggctggaat gccctgggca ggatgtggga gagccgcagc cacaccctcg
     3661 ctcagtgcct tggcttccag gagttccaga aagatgccaa gcaggctgaa gccatcctca
     3721 gcaaccagga atacactctg gctcacttgg agcccccaga ctccctggaa gctgcagagg
     3781 ctgggatccg gaagtttgag gatttcttgg ggtctatgga gaacaaccgg gataaggtct
     3841 tgagtcctgt ggactctgga aacaagctgg tagctgaggg aaacctatac tcagacaaga
     3901 tcaaggagaa ggtgcagctg attgaggaca ggcacaggaa gaacaacgag aaggcccagg
     3961 aggcctctgt cctactgaga gacaacctgg agctacagaa cttcctccag aactgccagg
     4021 agctcactct ctggatcaac gacaagctgc tgacatctca ggatgtctcc tatgatgaag
     4081 cacgaaacct tcacaataaa tggctaaagc accaggcgtt tgtggcagag ctggcttccc
     4141 atgaagggtg gctagagaac atcgatgcgg aaggaaagca gctgatggat gagaagcccc
     4201 agtttacagc cctggtgtcc caaaagctgg aagccctgca ccggctctgg gacgagctgc
     4261 aggccaccac aaaggagaag acccagcacc tctcggctgc caggagctcc gacctgcgct
     4321 tgcagaccca tgctgacctc aacaagtgga tcagcgccat ggaggaccag ctgcggtcag
     4381 acgacccggg caaggacctg accagtgtca atcggatgtt ggctaagctg aagcgagtgg
     4441 aggaccaagt gaatgtgcga aaagaggagc tgggggagct gtttgcccag gtgccttcaa
     4501 tgggagagga gggaggagat gcagacttga gcatcgagaa gcggttcctg gacctcctgg
     4561 aacccctagg aaggaggaag aagcagctgg aatcatccag agccaagctg cagatcagcc
     4621 gggacttaga ggatgagacg ctttgggtgg aggagaggct gcctctggcc cagtcagccg
     4681 actatggcac taatctgcaa actgtgcaac tgttcatgaa gaagaaccag acactgcaga
     4741 atgagattct gggccatacg ccgcgggttg aggatgtgct gcagagaggg cagcagctgg
     4801 tggaggcggc ggagatcgac tgccaggacc ttgaggagcg cctggggcac ctgcagagct
     4861 cctgggacag gctgcgggag gcagcggccg ggaggctgca gcgactgagg gacgccaacg
     4921 aggcacagca gtactacctg gatgcagacg aggctgaggc ctggattggc gagcaggagc
     4981 tctacgtcat ctccgatgag atccccaagg atgaagaggg cgccattgtg atgctgaagc
     5041 gacatttgcg gcagcagcgt gcggtggagg actacggccg gaacatcaag cagctggcca
     5101 gccgggccca gggcctgctg tctgcaggcc accctgaggg ggaacagatc atcagacttc
     5161 aggggcaagt ggacaagcac tacgcagggc tgaaggacgt ggcggaagag cgcaagcgca
     5221 agctggagaa catgtaccac ctgttccagc tcaagcggga gaccgacgac ctggagcagt
     5281 ggatttcaga aaaggagcta gtggcctctt ccccggaaat ggggcaagac tttgaccacg
     5341 tgactcttct gcgggacaag ttccgggact ttgcccggga gaccggggcg attgggcagg
     5401 agcgggtgga caatgtgaat gccttcatcg agcgactcat cgacgcgggc cacagcgagg
     5461 cggccaccat cgccgagtgg aaggacgggc tgaacgagat gtgggcagac ctcctggagc
     5521 tcattgacac gcgcatgcag ctgctggccg cctcctatga cctgcaccgc tacttctaca
     5581 cgggtgccga gatcctgggc ctcatcgacg agaagcaccg cgagctgccc gaggacgtgg
     5641 ggctggacgc cagcacggcc gagtccttcc accgggtgca cacagccttc gagcgggagc
     5701 tccacctgct gggtgtccag gtgcagcagt tccaggacgt ggccacccgt ctgcagacag
     5761 catatgctgg ggagaaggca gaggccatcc agaacaagga gcaggaggtg tctgccgcgt
     5821 ggcaggcgct gctcgatgcc tgtgccgggc gccggaccca gctagtggac acggcggata
     5881 aattccgctt cttcagcatg gcccgtgacc tcctctcctg gatggagagc atcatccggc
     5941 agatcgagac ccaggagagg cccagggatg tctcctctgt ggaactgctc atgaagtatc
     6001 accagggcat caatgcagag attgaaaccc ggagcaagaa cttcagtgcc tgcctggagc
     6061 ttggcgagtc cctgctgcag cggcagcacc aggcctcaga ggagatccgc gagaaactgc
     6121 agcaggtgat gtccaggagg aaagagatga atgagaagtg ggaagcccgc tgggagcggc
     6181 tccgcatgtt gctggaggtg tgccagttct cgagggatgc ctctgtggct gaggcgtggc
     6241 tgattgccca ggagccctac ctggccagcg gggactttgg acacacagtg gacagtgtgg
     6301 agaagctcat caagaggcat gaggcttttg agaagtccac ggccagctgg gcagagcgct
     6361 ttgctgccct ggagaagccc accacgcttg agctgaaaga acgccagatt gcagagagac
     6421 ccgcagagga gactgggcct caagaggagg aaggcgagac agcaggggag gctccagttt
     6481 cccaccatgc ggccaccgag agaacgtccc cgggggaaga agagggaacg tggcctcaga
     6541 acctgcagca gccaccaccg ccgggtcagc acaaggatgg gcagaaatcc actggggatg
     6601 agaggcccac cacggagccc ctctttaagg tcctagatac gcctctgagc gagggtgatg
     6661 agcccgcaac gctgccggcc ccgcgggacc atgggcagag tgtgcagatg gaaggctacc
     6721 tgggccgcaa gcatgacctg gaggggccca acaagaaggc ttccaacagg tcctggaaca
     6781 acctgtactg tgtgctcagg aacagtgagc taaccttcta caaggatgcc aagaacctgg
     6841 ccctggggat gccctaccat ggggaggaac ccctggccct gagacatgcc atctgtgaga
     6901 ttgctgccaa ctacaagaag aagaagcacg tctttaagct gaggctgagt aatggcagcg
     6961 agtggctctt ccatggcaag gatgaggagg agatgctgtc ctggctgcag ggcgtgagca
     7021 ccgccatcaa cgagtcccag agcatccgcg tcaaggcgca gagcctgccc ctgccttccc
     7081 tctccggccc cgacgccagc ctcggcaaga aagacaagga gaagagattc agcttcttcc
     7141 ccaaaaagaa gtagcaggtg gggctggcgg gcgcggcgga gtcgggacgc gcagagaccg
     7201 ggcagcccag gccttggcct cctctcgccg ccgcccgccg gtcgaatcga gatgagtcga
     7261 gtcgcgggcc gggcccccgg gccccacgct gcactgcaaa agctgccttc gcccgcgtgt
     7321 cctgggccga cgccccctcc atgccctcgc ccgcgctggc tccgctccag ggacaggggc
     7381 tcccagctcc tctggcgcct ttggggcctc caggcctggg ggcaggaaac ggttctctga
     7441 ggcacacgcc ctcctctccc accctcctcc tgagccgagg gggcacaggc cccttacccc
     7501 caaccaaaaa aaaaaaaaaa aaagagctgg agaaggctga tggggttctt caaacgctgc
     7561 ccgttcctaa agcaagtctt gcttcgggtc acctcccacc tggtggcagc cagggaaagg
     7621 ggaaaggaag aagacactgg aaatgcatgg ccagccccct aggggcatga ggaaggagcc
     7681 ttcaggtggc ccacaaagcc ctagctctgg gccaggggct ctggggggct gaggggacca
     7741 gactgggtgc agggccttgg gagctgccag cctccttccc actgggcttc cgcagaactg
     7801 ggactctcac ttcaggggcc accacatccc tcctctctgc ttctcccccc agatcaaagg
     7861 gtaccctccc acggttggca gggcctggct gagtgcctct agcacccttt gtgccaccac
     7921 aggcggtccc aggaagggca gcaaggtcag accattcctc attgaaaacc gtggctaggg
     7981 cacagggctc tgatctgaag gagtgacagg tgcgtcacaa cactgcctaa ggctctccct
     8041 ctcaatgctg ggaccctcgc cccactccca gtggcagcca aaccagcaat atccaacaga
     8101 cataggcccc ctacgggaca gtgtgggccc ccagatataa gatggatacc ccccgcacct
     8161 gcagtaaagc atcccaaagc caaatgctca attgcatcct gtctgcagct gggactttgg
     8221 agggtaatgg ctgggcagac agtaaagttg atcatctgtt ttctcggggt ggcagacgca
     8281 gccatgtctt ggtatcacca cccatgtgac agctactcct gcacaactgg gacaaatgtc
     8341 agggccagag ccatgaaggt gtggtacccc ttgatcagct tctccacgtc ctggccccag
     8401 gtcttggcgg ggagggggtc acatgagtac agaggtcaaa tatctaccac cctccagccc
     8461 tgagcaccag aagctctgca accaacgtcc ccaggaatgg ccttcccctt gctgctcagg
     8521 tcatacttct ggaggaagag aaagctcatt ccatattttc cccaccactc tactccgaaa
     8581 gcaacacagt tctggcccag tatcaaacta gggttaggtc caagagagca gtcaagcggg
     8641 tcgggggtgg gggggagggc ggagggcagc aggcattttc ccccaaggaa accatgaaag
     8701 tccccagggc ctgcagtcaa gtttctatca ccttccccgt gagaaggacc ttagttccat
     8761 ggagatgaag tgggtgaggt tcaggtagaa gccacagggg caggcatcca gaatgaggcc
     8821 ccctgaggaa ccctgagtgc tgaccagtgc cggcagaaat cagttccacc ctgtggccct
     8881 gacactatat gtcagggtga caccaactgc agcaggcagg agccacagga aggaagacgt
     8941 catccgatgg agcctgacat ccccaggttc ccgccctggt gagcaaggtc ttcatttggc
     9001 tttggtacat atgaccctat acagggaacc tagtgtgtat cactcaccag cctctttctg
     9061 tatctgggga aaccaaggca gtgaggtgac tgcactcacc tagcaataaa gtaggccttt
     9121 ggtgcacttg caggcatgtc ctcctctggg gcagagagca ggttccttgc tgaagggcag
     9181 atggtggcaa acatctccct caactgtttc cctgggccag tctttaggga gcctagggac
     9241 aagggcccac cctccccctt ccacgtgtgc caaatcagca cctgaagtga ggcatttcca
     9301 gcaagcaact ccacagaccc gagaaaggaa gggctctagt ggaagagcta gcatctcacc
     9361 accccaggtc ccctggcctg gtccagggca gccagctgca tgtgaccatt ctgttgccag
     9421 ctccccacac atccctcctg tcacccaggc tccaagacat tcgaccaact agactgaaaa
     9481 tgcaggcatc cagctttggg agaccctgtg tgtgccacac cagggctaag ctctcccagt
     9541 gccctagcaa ggtctttttt tttttttctc tccttattct actctacagt cttttgacag
     9601 tattcccaaa cgaggttgac acagctccag tccacaccag cataccaggc ttccttccca
     9661 gggcagttta gaggaagctc cttctgggca aggtcaggcg gtcctccttc cctctctcct
     9721 tcccctttgt cccagcctca actgactctg gctgtgggag gtgtggaggg tccttaggct
     9781 tccctcccca acctggcctc caccaacacc cctaacagga ggcccgtgga aggctcagcc
     9841 tctcctccgc atccctcctc cttcctgcct atcggaggga gccagggtcc cctaggctga
     9901 ccctgaatcc tcttcctccc ttcatgggag gggggcagga atccagagga ggatgaagcc
     9961 agcgggacca catggcttgg tggcttggac aaacaagctc agggaggaaa tgaggaggcg
    10021 gcggctgcag aggaatgcaa ccctgttggg cacagaccat gcaatctggg gctggccctg
    10081 gggccagctg ggtcctgtcc tccacctgct caaccgtgct tccaccaacc agaggaaacc
    10141 catttactag tttttctaat aaatcaataa tgctcca
//
