LOCUS       NM_080862               2963 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens splA/ryanodine receptor domain and SOCS box containing
            4 (SPSB4), mRNA.
ACCESSION   NM_080862
VERSION     NM_080862.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 2963)
  AUTHORS   Mekbib,T., Suen,T.C., Rollins-Hairston,A. and DeBruyne,J.P.
  TITLE     The E3 Ligases Spsb1 and Spsb4 Regulate RevErbalpha Degradation and
            Circadian Period
  JOURNAL   J Biol Rhythms 34 (6), 610-621 (2019)
   PUBMED   31607207
  REMARK    GeneRIF: Our results indicate that the paralogs Spsb1 and Spsb4,
            but not Spsb2 and Spsb3, can interact with and facilitate
            RevErbalpha ubiquitination and degradation and regulate circadian
            clock periodicity.
REFERENCE   4  (bases 1 to 2963)
  AUTHORS   Okumura,F., Joo-Okumura,A., Obara,K., Petersen,A., Nishikimi,A.,
            Fukui,Y., Nakatsukasa,K. and Kamura,T.
  TITLE     Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated
            by EphB2
  JOURNAL   Mol Biol Cell 28 (24), 3532-3541 (2017)
   PUBMED   28931592
REFERENCE   5  (bases 1 to 2963)
  AUTHORS   DeBruyne,J.P., Baggs,J.E., Sato,T.K. and Hogenesch,J.B.
  TITLE     Ubiquitin ligase Siah2 regulates RevErbalpha degradation and the
            mammalian circadian clock
  JOURNAL   Proc Natl Acad Sci U S A 112 (40), 12420-12425 (2015)
   PUBMED   26392558
REFERENCE   6  (bases 1 to 2963)
  AUTHORS   Nishiya,T., Matsumoto,K., Maekawa,S., Kajita,E., Horinouchi,T.,
            Fujimuro,M., Ogasawara,K., Uehara,T. and Miwa,S.
  TITLE     Regulation of inducible nitric-oxide synthase by the SPRY domain-
            and SOCS box-containing proteins
  JOURNAL   J Biol Chem 286 (11), 9009-9019 (2011)
   PUBMED   21199876
REFERENCE   7  (bases 1 to 2963)
  AUTHORS   Filippakopoulos,P., Low,A., Sharpe,T.D., Uppenberg,J., Yao,S.,
            Kuang,Z., Savitsky,P., Lewis,R.S., Nicholson,S.E., Norton,R.S. and
            Bullock,A.N.
  TITLE     Structural basis for Par-4 recognition by the SPRY domain- and SOCS
            box-containing proteins SPSB1, SPSB2, and SPSB4
  JOURNAL   J Mol Biol 401 (3), 389-402 (2010)
   PUBMED   20561531
REFERENCE   8  (bases 1 to 2963)
  AUTHORS   Lievens,S., Vanderroost,N., Van der Heyden,J., Gesellchen,V.,
            Vidal,M. and Tavernier,J.
  TITLE     Array MAPPIT: high-throughput interactome analysis in mammalian
            cells
  JOURNAL   J Proteome Res 8 (2), 877-886 (2009)
   PUBMED   19159283
REFERENCE   9  (bases 1 to 2963)
  AUTHORS   Kamura,T., Maenaka,K., Kotoshiba,S., Matsumoto,M., Kohda,D.,
            Conaway,R.C., Conaway,J.W. and Nakayama,K.I.
  TITLE     VHL-box and SOCS-box domains determine binding specificity for
            Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases
  JOURNAL   Genes Dev 18 (24), 3055-3065 (2004)
   PUBMED   15601820
REFERENCE   10 (bases 1 to 2963)
  AUTHORS   Kile,B.T., Schulman,B.A., Alexander,W.S., Nicola,N.A., Martin,H.M.
            and Hilton,D.J.
  TITLE     The SOCS box: a tale of destruction and degradation
  JOURNAL   Trends Biochem Sci 27 (5), 235-241 (2002)
   PUBMED   12076535
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC108727.6, BX376865.2, CN366621.1, BC051354.1, AF403029.1 and
            AC022215.4.
            
            On Nov 23, 2018 this sequence version replaced NM_080862.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008324.1, AK056367.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2145893
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000310546.3/ ENSP00000311609.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                AC108727.6         75374-75428         c
            56-65               BX376865.2         1-10
            66-134              CN366621.1         3-71
            135-135             AC108727.6         75294-75294         c
            136-395             CN366621.1         73-332
            396-396             AC108727.6         75033-75033         c
            397-554             BC051354.1         40-197
            555-2358            AF403029.1         10-1813
            2359-2359           AC022215.4         136950-136950       c
            2360-2963           AF403029.1         1815-2418
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q23"
     gene            1..2963
                     /gene="SPSB4"
                     /gene_synonym="SSB-4; SSB4"
                     /note="splA/ryanodine receptor domain and SOCS box
                     containing 4"
                     /db_xref="GeneID:92369"
                     /db_xref="HGNC:HGNC:30630"
                     /db_xref="MIM:611660"
     exon            1..646
                     /gene="SPSB4"
                     /gene_synonym="SSB-4; SSB4"
                     /inference="alignment:Splign:2.1.0"
     exon            647..1493
                     /gene="SPSB4"
                     /gene_synonym="SSB-4; SSB4"
                     /inference="alignment:Splign:2.1.0"
     CDS             800..1621
                     /gene="SPSB4"
                     /gene_synonym="SSB-4; SSB4"
                     /note="SPRY domain-containing SOCS box protein SSB-4"
                     /codon_start=1
                     /product="SPRY domain-containing SOCS box protein 4"
                     /protein_id="NP_543138.1"
                     /db_xref="CCDS:CCDS3115.1"
                     /db_xref="GeneID:92369"
                     /db_xref="HGNC:HGNC:30630"
                     /db_xref="MIM:611660"
                     /translation="MGQKLSGSLKSVEVREPALRPAKRELRGAEPGRPARLDQLLDMP
                     AAGLAVQLRHAWNPEDRSLNVFVKDDDRLTFHRHPVAQSTDGIRGKVGHARGLHAWQI
                     NWPARQRGTHAVVGVATARAPLHSVGYTALVGSDAESWGWDLGRSRLYHDGKNQPGVA
                     YPAFLGPDEAFALPDSLLVVLDMDEGTLSFIVDGQYLGVAFRGLKGKKLYPVVSAVWG
                     HCEVTMRYINGLDPEPLPLMDLCRRSIRSALGRQRLQDISSLPLPQSLKNYLQYQ"
     exon            1494..2963
                     /gene="SPSB4"
                     /gene_synonym="SSB-4; SSB4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2938..2943
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPSB4"
                     /gene_synonym="SSB-4; SSB4"
                     /note="hexamer: AATAAA"
     polyA_site      2963
                     /gene="SPSB4"
                     /gene_synonym="SSB-4; SSB4"
                     /note="major polyA site"
ORIGIN      
        1 agatcggctt tcagctgcag cctccgggcc ggccgggaag gcggggagcg ggcggcggcg
       61 gcggaggagg gggaggcggc ggcggctgca gcatccagag ctggccgtgg cggccggcgc
      121 gccccgcgca caaaagcacc cagccccagg ggagggcgat gaacacacca catcccgggc
      181 ccgggcccca gctgctgcta ccgctgcgtg cgctcagggc gctggggaag acgcccggcg
      241 cgccgggggc cagcggccga ggcgcggccc gtgcgccctg agcgcgggac tcgtcgccct
      301 ccgggtcagg cgccaagctt ccaagcggct agagcgcggg ccttggagcg cccccaggat
      361 cgcttcagta aggcgcttcc ccactccagg cccgaccccc ggcgcctgag cgccaacttc
      421 gccaagaacg ctcctaactc caggccatcc tgcagcgcag agggggcgct gctgccgggc
      481 atcagccgtg aggacgcgcc cctggccgtg cggagagagc cggcatttgc gggtcactcg
      541 ggcgcccctg agtgggcggc ggcggcagca gacccctctc cagggagtcc aggacctgcc
      601 agcgctgggg attcttcccg aacaggcgct tgccctctct tttatggagc ttgggcccct
      661 gccgcagccc ggtagaggct gtggaggtct accgtccgga agcctggttc ccagccccgt
      721 ggcccattcc tggctacggg gagtggaggc tcccacgagg tagcggtggc ctgcagcggc
      781 ctcctccccg cagtgaagca tgggccagaa gctctcgggg agcctcaagt cagtggaggt
      841 gcgagagccg gcgctgcggc cggccaagcg ggagctgcgg ggtgcagagc ccgggcggcc
      901 ggcgcggctg gaccagctgt tggacatgcc agcggcgggg ctggctgtgc agctgcggca
      961 cgcgtggaac cccgaggacc gctcgctcaa cgtcttcgtc aaggacgacg accggctcac
     1021 cttccaccgg caccccgtgg cccagagcac cgacggcatc cgcggcaagg tgggccacgc
     1081 ccgcggcctg cacgcctggc agatcaactg gccggctcgg cagcgcggca cccacgctgt
     1141 agttggtgtg gccacggccc gtgctcccct gcactccgtg ggctacacgg cgctggtagg
     1201 cagtgacgcc gagtcgtggg gctgggacct gggccgcagc cgcctctacc acgacggcaa
     1261 gaaccagccc ggcgtggcct acccggcctt tctggggccc gacgaggcct ttgcgctgcc
     1321 cgactcgctg ctcgtggtgc tggacatgga tgagggcaca ctcagcttca tcgtggatgg
     1381 ccagtacctg ggcgtggcct tccgaggtct caagggcaag aagctgtacc cggtggtgag
     1441 tgccgtgtgg ggccactgtg aagtcaccat gcgctacatc aacggccttg accccgagcc
     1501 cctgccactg atggacctgt gccggagatc catccgctcg gccctgggcc gccagcgcct
     1561 gcaggacatc agctccctgc ccctgcctca gtctctcaaa aactatctgc agtaccagtg
     1621 agccaagcct gatgggcagc acagacacag acacacaccg cagggcccga ccctcctgtc
     1681 attcacagtc ccatggcaca taggggaaag gatctaccct tctcctggct ccccaggaca
     1741 ctcagttctt tcaaagacca ggatgtggta ccaactttgg aaacgaaagg tctcttgcca
     1801 acagtatcta ctgccctcga ggcagccctc ccaagtcaga cacctccttc ggagccacag
     1861 agagcctgga gtctgcacct cctggaaatc ctgccaccaa ccaggacaca gcagccaccg
     1921 tattgatcag agagcctgtt tccttattca agagaatgaa taaaacattt aggcaggaga
     1981 ctttctattg tgtgccccgt tgcagacagg gccagggaga aattagccag tgcaggggga
     2041 aaattgcctc tgaataatga atgatgaaag cctgacgccg tgcccctcct ggcccatacg
     2101 ccttgccagg gcggcaggat tgtcacaccg ttttaggact ctgtgccact ttgagagact
     2161 gttcccagga ggcccaaccg cagacctggc aagtggacag tgcagtgtgg agacaccttc
     2221 cggcttacct ctttgaacgt tgtttaccta cccctttcca cgtgctcccc ttcccagcca
     2281 ctgactcaca gttctctgga tgcccagaca cctctcttca gggaagatga gtctgactgg
     2341 tttgccccag ggaagagcat atccttacca ttattttaag tagcatttgc attttaaaag
     2401 aggaatgcgg agaggacagt acttagtcca aaggtgctaa cgggggaact gggggcattg
     2461 tgacacccaa gtctgatgtg tcctgggttg gggggccttc ctggagtgtc agggtctctg
     2521 ggcaacgtcc attcagggtg cggcatggct gtcacaaagc tttatttgag caaattattt
     2581 tttcacttta ggagacttct acaagtttgt ttcctgtttc aaatgtgtgt gtgatgtgct
     2641 gtttatttat cagcttgagg tccatggggg cagccttgtg actggaaggg tggatatggg
     2701 agacacattc tctacctgct ccgagcctgg tcctctcgca ggaatgctgc tgctgcctcc
     2761 gccgccactg ctgctgccac ctcttatatg tttcagacac tctctgccca gactcatttt
     2821 taactggaaa tcatcacagc agtgggatat cagagcccca gacagcactg cctcttcctc
     2881 cccaccccac tgcccccacc ttaatgtgaa tttgactgat gaatgaagag cgtttctaat
     2941 aaagtttgtc attcagtcct tca
//
