LOCUS       NM_032641               1203 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens splA/ryanodine receptor domain and SOCS box containing
            2 (SPSB2), transcript variant 1, mRNA.
ACCESSION   NM_032641
VERSION     NM_032641.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Li,K., You,T., Zhao,P., Luo,Y., Zhang,D., Wei,H., Wang,Y., Yang,J.,
            Guan,X. and Kuang,Z.
  TITLE     Structural basis for the regulation of inducible nitric oxide
            synthase by the SPRY domain-containing SOCS box protein SPSB2, an
            E3 ubiquitin ligase
  JOURNAL   Nitric Oxide 113-114, 1-6 (2021)
   PUBMED   33862200
  REMARK    GeneRIF: Structural basis for the regulation of inducible nitric
            oxide synthase by the SPRY domain-containing SOCS box protein
            SPSB2, an E3 ubiquitin ligase.
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1203)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 1203)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1203)
  AUTHORS   Wang,M., Wang,Y., Liu,Y., Wang,H., Xin,X., Li,J., Hao,Y., Han,L.,
            Yu,F., Zheng,C. and Shen,C.
  TITLE     SPSB2 inhibits hepatitis C virus replication by targeting NS5A for
            ubiquitination and degradation
  JOURNAL   PLoS One 14 (7), e0219989 (2019)
   PUBMED   31344133
  REMARK    GeneRIF: Results demonstrated that cellular factor SPSB2 interacts
            with and decreases viral protein NS5A to inhibit HCV replication.
            SPSBP2 interacts with the HCV protein NS5A via its SPRY domain and
            inhibits HCV replication by ubiquitination and degradation of NS5A.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1203)
  AUTHORS   Wang,D., Li,Z., Messing,E.M. and Wu,G.
  TITLE     The SPRY domain-containing SOCS box protein 1 (SSB-1) interacts
            with MET and enhances the hepatocyte growth factor-induced
            Erk-Elk-1-serum response element pathway
  JOURNAL   J Biol Chem 280 (16), 16393-16401 (2005)
   PUBMED   15713673
REFERENCE   7  (bases 1 to 1203)
  AUTHORS   Kamura,T., Maenaka,K., Kotoshiba,S., Matsumoto,M., Kohda,D.,
            Conaway,R.C., Conaway,J.W. and Nakayama,K.I.
  TITLE     VHL-box and SOCS-box domains determine binding specificity for
            Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases
  JOURNAL   Genes Dev 18 (24), 3055-3065 (2004)
   PUBMED   15601820
REFERENCE   8  (bases 1 to 1203)
  AUTHORS   Kile,B.T., Schulman,B.A., Alexander,W.S., Nicola,N.A., Martin,H.M.
            and Hilton,D.J.
  TITLE     The SOCS box: a tale of destruction and degradation
  JOURNAL   Trends Biochem Sci 27 (5), 235-241 (2002)
   PUBMED   12076535
  REMARK    Review article
REFERENCE   9  (bases 1 to 1203)
  AUTHORS   Ansari-Lari,M.A., Shen,Y., Muzny,D.M., Lee,W. and Gibbs,R.A.
  TITLE     Large-scale sequencing in human chromosome 12p13: experimental and
            computational gene structure determination
  JOURNAL   Genome Res 7 (3), 268-280 (1997)
   PUBMED   9074930
REFERENCE   10 (bases 1 to 1203)
  AUTHORS   Ansari-Lari,M.A., Muzny,D.M., Lu,J., Lu,F., Lilley,C.E., Spanos,S.,
            Malley,T. and Gibbs,R.A.
  TITLE     A gene-rich cluster between the CD4 and triosephosphate isomerase
            genes at human chromosome 12p13
  JOURNAL   Genome Res 6 (4), 314-326 (1996)
   PUBMED   8723724
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DT220163.1 and BC002983.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_032641.3.
            
            Summary: This gene encodes a member of a subfamily of proteins
            containing a central SPRY (repeats in splA and RyR) domain and a
            C-terminal suppressor of cytokine signaling (SOCS) box. This
            protein plays a role in cell signaling. This gene is present in a
            gene-rich cluster on chromosome 12p13 in the vicinity of the CD4
            antigen and triosephosphate isomerase genes. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Feb
            2016].
            
            Transcript Variant: This variant (1) is the reference variant.
            Variants 1, 2, and 4 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC002983.1, SRR14038194.644664.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000524270.6/ ENSP00000428338.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                DT220163.1         58-72
            16-1203             BC002983.1         1-1188
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..1203
                     /gene="SPSB2"
                     /gene_synonym="GRCC9; SSB2"
                     /note="splA/ryanodine receptor domain and SOCS box
                     containing 2"
                     /db_xref="GeneID:84727"
                     /db_xref="HGNC:HGNC:29522"
                     /db_xref="MIM:611658"
     exon            1..58
                     /gene="SPSB2"
                     /gene_synonym="GRCC9; SSB2"
                     /inference="alignment:Splign:2.1.0"
     exon            59..818
                     /gene="SPSB2"
                     /gene_synonym="GRCC9; SSB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..946
                     /gene="SPSB2"
                     /gene_synonym="GRCC9; SSB2"
                     /note="SPRY domain-containing SOCS box protein SSB-2;
                     gene-rich cluster protein C9"
                     /codon_start=1
                     /product="SPRY domain-containing SOCS box protein 2"
                     /protein_id="NP_116030.1"
                     /db_xref="CCDS:CCDS8567.1"
                     /db_xref="GeneID:84727"
                     /db_xref="HGNC:HGNC:29522"
                     /db_xref="MIM:611658"
                     /translation="MGQTALAGGSSSTPTPQALYPDLSCPEGLEELLSAPPPDLGAQR
                     RHGWNPKDCSENIEVKEGGLYFERRPVAQSTDGARGKRGYSRGLHAWEISWPLEQRGT
                     HAVVGVATALAPLQTDHYAALLGSNSESWGWDIGRGKLYHQSKGPGAPQYPAGTQGEQ
                     LEVPERLLVVLDMEEGTLGYAIGGTYLGPAFRGLKGRTLYPAVSAVWGQCQVRIRYLG
                     ERRAEPHSLLHLSRLCVRHNLGDTRLGQVSALPLPPAMKRYLLYQ"
     exon            819..1203
                     /gene="SPSB2"
                     /gene_synonym="GRCC9; SSB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1172..1177
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPSB2"
                     /gene_synonym="GRCC9; SSB2"
                     /note="hexamer: AGTAAA"
     polyA_site      1203
                     /gene="SPSB2"
                     /gene_synonym="GRCC9; SSB2"
                     /note="major polyA site"
ORIGIN      
        1 ctttccgccc ggctccttca gaggcccggc gacctccagg gctgggaagt caaccgagct
       61 cccttccagg tcaatccaaa ctggagctca actttcagaa gagaaagacg ccccagcaag
      121 cctctttcgg ggagtcctct agctcctcac ctccatgggc cagacagctc tggcaggggg
      181 cagcagcagc acccccacgc cacaggccct gtaccctgac ctctcctgtc ccgagggctt
      241 ggaagagctg ctgtctgcac cccctcctga cctgggggcc cagcggcgcc acggttggaa
      301 ccccaaagac tgttcagaga acatcgaggt caaggaagga gggttgtact ttgagcggcg
      361 gcccgtggcc cagagcactg atggggcccg gggtaagagg ggctattcaa ggggcctgca
      421 cgcctgggag atcagctggc ccctagagca gaggggcacg catgccgtgg tgggcgtggc
      481 cacggccctc gccccgctgc agactgacca ctacgcggcg ctgctgggca gcaacagcga
      541 gtcgtggggc tgggacatcg ggcgggggaa gctgtaccat cagagcaagg ggcccggagc
      601 cccccagtat ccagcgggaa ctcagggtga gcagctggag gtgccagaga gactgctggt
      661 ggttctggac atggaggagg gaactctggg ctacgctatt gggggcacct acctggggcc
      721 agcattccgc ggactgaagg gcaggaccct ctatccggca gtaagcgctg tctggggcca
      781 gtgccaggtc cgcatccgct acctgggcga aaggagagcg gagccacact cccttctgca
      841 cctgagccgc ctgtgtgtgc gccacaacct gggggatacc cggctcggcc aggtgtctgc
      901 cctgcccttg ccccctgcca tgaagcgcta cctgctctac cagtgagccc tgtgatacca
      961 cagactgtgc tgaggtcttg ccaccacccc tccccttggg gaggtgggga ggcactgctg
     1021 gcctagacca gctgctgaaa gctggtgagg ctgagcccct accccaaccc aagctctgcg
     1081 gaaatcaaca gccccagagc cacttggagg gaggaagaaa gggagccggc gttcaaggct
     1141 atgacagtct gctacgcaaa acattttttc aagtaaaaat agtaagagat gttgttatag
     1201 aaa
//
