LOCUS       NM_207344              10769 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens SPRY domain containing 4 (SPRYD4), mRNA.
ACCESSION   NM_207344
VERSION     NM_207344.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Ma,Z., Xie,T., Sun,J., Yu,J., Huang,S., Zhou,Q. and Li,B.
  TITLE     Identification of SPRYD4 as a tumour suppressor predicts prognosis
            and correlates with immune infiltration in cholangiocarcinoma
  JOURNAL   BMC Cancer 23 (1), 404 (2023)
   PUBMED   37142983
  REMARK    GeneRIF: Identification of SPRYD4 as a tumour suppressor predicts
            prognosis and correlates with immune infiltration in
            cholangiocarcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10769)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 10769)
  AUTHORS   Chen,Y., Chen,S., Lu,J., Yuan,D., He,L., Qin,P., Tan,H. and Xu,L.
  TITLE     MicroRNA-363-3p promote the development of acute myeloid leukemia
            with RUNX1 mutation by targeting SPRYD4 and FNDC3B
  JOURNAL   Medicine (Baltimore) 100 (18), e25807 (2021)
   PUBMED   33950983
  REMARK    GeneRIF: MicroRNA-363-3p promote the development of acute myeloid
            leukemia with RUNX1 mutation by targeting SPRYD4 and FNDC3B.
REFERENCE   4  (bases 1 to 10769)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 10769)
  AUTHORS   Zahid,K.R., Han,S., Zhou,F. and Raza,U.
  TITLE     Novel tumor suppressor SPRYD4 inhibits tumor progression in
            hepatocellular carcinoma by inducing apoptotic cell death
  JOURNAL   Cell Oncol (Dordr) 42 (1), 55-66 (2019)
   PUBMED   30238408
  REMARK    GeneRIF: SPRYD4 expression may serve as a biomarker for a good
            overall and relapse-free survival in hepatocellular carcinoma
            patients.
REFERENCE   6  (bases 1 to 10769)
  AUTHORS   Cheng,C.Y., Schache,M., Ikram,M.K., Young,T.L., Guggenheim,J.A.,
            Vitart,V., MacGregor,S., Verhoeven,V.J., Barathi,V.A., Liao,J.,
            Hysi,P.G., Bailey-Wilson,J.E., St Pourcain,B., Kemp,J.P.,
            McMahon,G., Timpson,N.J., Evans,D.M., Montgomery,G.W., Mishra,A.,
            Wang,Y.X., Wang,J.J., Rochtchina,E., Polasek,O., Wright,A.F.,
            Amin,N., van Leeuwen,E.M., Wilson,J.F., Pennell,C.E., van
            Duijn,C.M., de Jong,P.T., Vingerling,J.R., Zhou,X., Chen,P., Li,R.,
            Tay,W.T., Zheng,Y., Chew,M., Burdon,K.P., Craig,J.E., Iyengar,S.K.,
            Igo,R.P. Jr., Lass,J.H. Jr., Chew,E.Y., Haller,T., Mihailov,E.,
            Metspalu,A., Wedenoja,J., Simpson,C.L., Wojciechowski,R., Hohn,R.,
            Mirshahi,A., Zeller,T., Pfeiffer,N., Lackner,K.J., Bettecken,T.,
            Meitinger,T., Oexle,K., Pirastu,M., Portas,L., Nag,A.,
            Williams,K.M., Yonova-Doing,E., Klein,R., Klein,B.E.,
            Hosseini,S.M., Paterson,A.D., Makela,K.M., Lehtimaki,T.,
            Kahonen,M., Raitakari,O., Yoshimura,N., Matsuda,F., Chen,L.J.,
            Pang,C.P., Yip,S.P., Yap,M.K., Meguro,A., Mizuki,N., Inoko,H.,
            Foster,P.J., Zhao,J.H., Vithana,E., Tai,E.S., Fan,Q., Xu,L.,
            Campbell,H., Fleck,B., Rudan,I., Aung,T., Hofman,A.,
            Uitterlinden,A.G., Bencic,G., Khor,C.C., Forward,H., Parssinen,O.,
            Mitchell,P., Rivadeneira,F., Hewitt,A.W., Williams,C., Oostra,B.A.,
            Teo,Y.Y., Hammond,C.J., Stambolian,D., Mackey,D.A., Klaver,C.C.,
            Wong,T.Y., Saw,S.M. and Baird,P.N.
  CONSRTM   Consortium for Refractive Error and Myopia; Fuchs' Genetics
            Multi-Center Study Group; Wellcome Trust Case Control Consortium 2;
            Diabetes Control and Complications Trial/Epidemiology of Diabetes
            Interventions, and Complications Research Group
  TITLE     Nine loci for ocular axial length identified through genome-wide
            association studies, including shared loci with refractive error
  JOURNAL   Am J Hum Genet 93 (2), 264-277 (2013)
   PUBMED   24144296
REFERENCE   7  (bases 1 to 10769)
  AUTHORS   Kettunen,J., Tukiainen,T., Sarin,A.P., Ortega-Alonso,A.,
            Tikkanen,E., Lyytikainen,L.P., Kangas,A.J., Soininen,P., Wurtz,P.,
            Silander,K., Dick,D.M., Rose,R.J., Savolainen,M.J., Viikari,J.,
            Kahonen,M., Lehtimaki,T., Pietilainen,K.H., Inouye,M.,
            McCarthy,M.I., Jula,A., Eriksson,J., Raitakari,O.T., Salomaa,V.,
            Kaprio,J., Jarvelin,M.R., Peltonen,L., Perola,M., Freimer,N.B.,
            Ala-Korpela,M., Palotie,A. and Ripatti,S.
  TITLE     Genome-wide association study identifies multiple loci influencing
            human serum metabolite levels
  JOURNAL   Nat Genet 44 (3), 269-276 (2012)
   PUBMED   22286219
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 10769)
  AUTHORS   Inouye,M., Ripatti,S., Kettunen,J., Lyytikainen,L.P., Oksala,N.,
            Laurila,P.P., Kangas,A.J., Soininen,P., Savolainen,M.J.,
            Viikari,J., Kahonen,M., Perola,M., Salomaa,V., Raitakari,O.,
            Lehtimaki,T., Taskinen,M.R., Jarvelin,M.R., Ala-Korpela,M.,
            Palotie,A. and de Bakker,P.I.
  TITLE     Novel Loci for metabolic networks and multi-tissue expression
            studies reveal genes for atherosclerosis
  JOURNAL   PLoS Genet 8 (8), e1002907 (2012)
   PUBMED   22916037
REFERENCE   9  (bases 1 to 10769)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 10769)
  AUTHORS   Zhong,Z., Zhang,H., Bai,M., Ni,J., Wan,B., Chen,X. and Yu,L.
  TITLE     Cloning and characterization of a novel human SPRYD4 gene encoding
            a putative SPRY domain-containing protein
  JOURNAL   DNA Seq 19 (1), 68-72 (2008)
   PUBMED   17852359
  REMARK    GeneRIF: Subcellular localization demonstrates that SPRYD4
            (SPRY-domain-containing) protein is localized in the nucleus when
            overexpressed in COS-7 green monkey cells.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB041397.1, BX647957.1, AL832247.1 and AC097104.18.
            
            On Nov 23, 2018 this sequence version replaced NM_207344.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC020844.1, AL832247.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000338146.7/ ENSP00000338034.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-461               DB041397.1         92-552
            462-1046            BX647957.1         460-1044
            1047-2046           AL832247.1         1061-2060
            2047-10769          AC097104.18        5738-14460
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.3"
     gene            1..10769
                     /gene="SPRYD4"
                     /note="SPRY domain containing 4"
                     /db_xref="GeneID:283377"
                     /db_xref="HGNC:HGNC:27468"
     exon            1..99
                     /gene="SPRYD4"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..638
                     /gene="SPRYD4"
                     /codon_start=1
                     /product="SPRY domain-containing protein 4"
                     /protein_id="NP_997227.1"
                     /db_xref="CCDS:CCDS8920.1"
                     /db_xref="GeneID:283377"
                     /db_xref="HGNC:HGNC:27468"
                     /translation="MALLFARSLRLCRWGAKRLGVASTEAQRGVSFKLEEKTAHSSLA
                     LFRDDTGVKYGLVGLEPTKVALNVERFREWAVVLADTAVTSGRHYWEVTVKRSQQFRI
                     GVADVDMSRDSCIGVDDRSWVFTYAQRKWYTMLANEKAPVEGIGQPEKVGLLLEYEAQ
                     KLSLVDVSQVSVVHTLQTDFRGPVVPAFALWDGELLTHSGLEVPEGL"
     exon            100..10769
                     /gene="SPRYD4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1055..1060
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRYD4"
                     /note="hexamer: AGTAAA"
     polyA_site      1081
                     /gene="SPRYD4"
     regulatory      2023..2028
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRYD4"
                     /note="hexamer: ATTAAA"
     polyA_site      2046
                     /gene="SPRYD4"
                     /note="major polyA site"
     regulatory      3694..3699
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRYD4"
                     /note="hexamer: AGTAAA"
     polyA_site      3713
                     /gene="SPRYD4"
     regulatory      4454..4459
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRYD4"
                     /note="hexamer: AGTAAA"
     polyA_site      4478
                     /gene="SPRYD4"
     regulatory      10473..10478
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRYD4"
                     /note="hexamer: AATAAA"
     polyA_site      10491
                     /gene="SPRYD4"
     regulatory      10731..10736
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRYD4"
                     /note="hexamer: AATAAA"
     polyA_site      10769
                     /gene="SPRYD4"
ORIGIN      
        1 atccaaggcg caagatggcg ctgctttttg cacgttcttt gcgcttgtgc cgctggggag
       61 ccaaacgatt gggagttgcc tccacagagg cccagagagg cgtcagtttc aaactggaag
      121 aaaaaaccgc ccacagcagc ctggcactct tcagagatga tacgggtgtc aaatatggct
      181 tggtgggatt ggagcccacc aaggtggcct tgaatgtgga gcgcttccgg gagtgggcag
      241 tggtgctggc agacacagcg gtcaccagtg gcagacacta ctgggaagtg acagtgaagc
      301 gctcccagca gttccggata ggagtggcag atgtggacat gtcccgggat agctgcattg
      361 gtgttgatga tcgttcctgg gtgttcacct atgcccagcg caagtggtac accatgttgg
      421 ccaacgagaa agccccagtt gagggtattg ggcagccaga gaaggtgggg ctgttgctgg
      481 agtatgaggc ccagaagctg agcctggtgg atgtgagcca ggtctctgtg gttcacacgc
      541 tacagacaga tttccggggt ccagtggtgc ctgcctttgc tctctgggat ggggagctgc
      601 tgacccattc agggcttgag gtgcccgagg gcctctagta tgtccattac tggagtccct
      661 aatcacgcct ttggccagcc tccttttgaa agtgtccgaa gcctttttac tttgcctcaa
      721 gcaacctcta gctcccacaa ttcagtgttg ggtcctctgt gcaatatcat gatcatcttc
      781 ctcatcccct accttgtgaa agctaggcat acagccaaac cctccttttc cccacccacc
      841 aactactgcc aatttcctag gctaccatgg gtgtatcttc cttgacctgc ttccttcagt
      901 ccctctgcct ccctttgccc aggcctttct cagactgtat tccatcctgg ggtcttatca
      961 ttcagctttg tttgaattta ttaatcacca tgatacctct ccctcccttt gtccacatgt
     1021 aacttgttct tggggctcta ccagatggct gaagagtaaa tcctttctac ctctggctga
     1081 aggagtggtg cagtcaatga cttggccctt tttctacagc acatttagag tttgggctct
     1141 ggctccctca gtaagtgctt tattaactca gtgtgtcaaa atgaaaacag agccctcctt
     1201 ccccagtagc tcagtgccac agaccttcag ccccacacag ctgtagctat ccttaccctg
     1261 agtccatcta ccttaaatct gtacctctga cacccagcca gtctgtcata atcattatcc
     1321 cagttataac cttgaccaaa ggggaagaga gacacttggg ggatatctag gggatgagtg
     1381 ctagaaacta tttatttatt tttgagacag agtctcactc tgtcacccag gctggagtgc
     1441 agtggcatga tcttggctca ctgcaaactc tgccttttgg gttcaagcga ctctcgtgcc
     1501 tcagcctcct gagtagctag gattacaggt gtgtgccacc atgcccgtct catttttata
     1561 tttttagtag agatggggtt ttgccatatt ggccaggctg gtctcgaact cctggcctca
     1621 tgtgatcagc ccaccttggc ctcccaaagt gctgggattt caggcgtgag ccaccacacc
     1681 cggccaaaac tgtttattct tgagaagttc catcttcatt tctgccacag ttggaacttc
     1741 ccgaggaagg aaggaggcct gaggttttgc acaatctgtt tcagagcctg tttagactca
     1801 aacctatgct tcccttggca gcagaataca cttaacctaa agcagtattt ggagttgaga
     1861 aaaacctggt ggggtaagtg aatatgtact gtttggtagg gtaggtagag aagctgtgct
     1921 ttgaccctgt gattccatct ttttctacct tctatgatgg tgatgaagct agatacccct
     1981 agggaagaaa gaaggactgg gtttagcaaa aatgatttgg taattaaagt ttatttgaac
     2041 acaaaatact ttctctgtct ataaaattgg ctgttagcag tagcagcagc atgtcctggc
     2101 caaggggagt agatttctcc agactactaa agccatgtat atagccattc ccacttccca
     2161 tattctgtgg atatgtacat gtgcatggta gctagagtcc ctccaccaga gtatctctct
     2221 catgatggtt tcttcaggga gaggaagagg agacctgggt gaagagctgg gatggttttg
     2281 agtggggcaa gccattaggc tgtaccttga agcccagtct ctctggatag ctgtactgca
     2341 ggtgtcctct gaggcccttc tctgtactct gtctgctgag ggaatggggt attttgactc
     2401 ccatagaaag cactagccta agtcaccaaa tgactgcttg gtccccactg aagcagtgta
     2461 gctctccata gtatttttgg tggttatgga ttacatgtgt ggccagctca tgctttttct
     2521 tgagcagggg ctgtccatga cctgtgctca taccatgctt tctaagttct ctttggacag
     2581 ggcctcagct gctgcctcag cctgagtttc agagagtgtg taggagtcct ggtaatcttg
     2641 aagcagtttg accacctcca gatggttgaa ctgcacagca tcatccaggg gaatgttgcc
     2701 ccacctgaga ggaataatga tatgatcagg caaaggagac gtggagagtg gtggttgtat
     2761 atatttgcat ggtggctgga gggtaggtgg agaagctgtg cccaccctcc tccactttta
     2821 gcctattcct cacctgtcct tggcaaaagg attcactttg caagcctcga tcaggaattt
     2881 aacaacttcg atgtgtccta ggaatatggg cagaaggaaa atgagaaggc gcaggtcttg
     2941 aaccctttgg tgcagacact tagccactga agtctttacc aagaggggag gggaaaaggc
     3001 ctcttcccat tttgtaccct gcagcactca gtcatagtct agctgcaaac cgaagggtgt
     3061 ctagataaaa ctagttgctg cccctataac cttgagggca catataatga aggtactttt
     3121 ggtgaaaaag aaagggtctt atgattaccc tcctcctccc ctccttaacc cttcagtacc
     3181 ttcagctgca gcaacatgca gagctgtgcg cgagtcatag tctttctgtt ccatatccat
     3241 ggctgacaag gcaaacctga gggtagtggg aaagcagcta gagttgccta gatcagactg
     3301 gaatcacagg tgttctttgt gaactggtgt cagactggat gagtttcaga gaaagtgaaa
     3361 cagcctatag ccagatttgt tggctctgaa taatcttttt tccatatcca gatgagactg
     3421 ttatactcat attagagaga tgggaatgtg atccttccag aaatatcact cactgcctac
     3481 ctgtggtaag tgcccatttg aggcagggta cctacttcct aggacactgc tcttaacact
     3541 caataacaat ggctaacatt aattatcata gagcagacag tttacttttt ttaaaaaaaa
     3601 tctccttttt taaaaaaatt tcatttaaat gcaatctata tccattctaa ttccaaagct
     3661 gaagcactta actattttgt tacgctgcct cctagtaaaa tctctgacca ggagtatttt
     3721 attgtgtata tttggtcaca gtagcattac cttcgaagag ctgagacatc gccactatag
     3781 gcagcaaata acaggttgac cacagtcttg ttctggaaca caaatcatac ccacatgaca
     3841 ttaattgaac tcacctatgc cagctgtgcc ctgtgaccct cctccatgga tgcttagtcc
     3901 aagggtattg ctgaagtgtt atggaatgtg ctactctgaa atattctttt tttttttttt
     3961 ttttttgaga cggagtctcg ctctgtcgtt caggctgaag tgtagtggcg cgcggtcttg
     4021 gctcactgca acctctgcct cccggtttca agcgattctc ctgcctcagc ctcccaagta
     4081 gctgggacca caggcgcgtg ccaccacgtc tggctaattt tttgtatttt caatagagac
     4141 caggtttcac cgtgttagcc aggatggtct tgatctcctg accttgtgat ccgcccgcct
     4201 tggcctctca aagtgcaggg attacaggcg tgagccaccg cacctggcct ttgaaatatt
     4261 cttacaagcc acctggagtt ttccttaccc gaatttctgc cccttcacgc cgtgggtcta
     4321 acttccgagc acagtgcctc aggttgtcat agttgtggaa attgaagaga gacaccaact
     4381 tctagaattg taagccaaat atattgttta tttactcctt acacttagta ggagctcaaa
     4441 attgctttat tatagtaaaa gtggtaccat taaacaaatt tattgcttca aaaatagtaa
     4501 gtactatatg caaagcatct cacctggcag aagctggtcc ccctatggct gttccccagc
     4561 ttgtccaatg ggggtgacag gcacatcatt cccatgacat tgggtaccac caggaggatg
     4621 gctcctgata cagctgactt ggctggcagg cccacctggg gaacagaact gaagctgagg
     4681 ataaagtggg tgtgccccaa atcagaaaat aaacaagtta ggctgtactc ttaacaagtt
     4741 tacaaatgac tgcatgacca caatccacct caaccttttg gcttgttaat tagcttcttt
     4801 tattactcct gtcctttcct tcccttaaat tcattgattc agctagaaaa tattttttga
     4861 aatacttaca gcattctgtg ctagatgctg tgctagaact aggaacttcc attctggtgt
     4921 taggagatag gtaataaaca ggtaaataaa tagacacgta atgtttaaag taattgtgat
     4981 aaatagtgag aagtagggtg ctgtaaatag aggtagaggg gggccacatg agatagggtg
     5041 gtcagaaaag tctttctttg gaaatgacat ctaagctgag accccaagga gaagagccag
     5101 caatgtgaag cacaattcag gagctgcaac acctctggtt gtttttcttt ttcttttttt
     5161 cttttttttt gagatggagt tttgctcttg ttgcccaggc tacagtgcaa tggcacaatc
     5221 tcggctcagt gcaacctctg cctcccaggt tcaagcactt ctgcctcagc ctcccaagta
     5281 gctgggatta caggtgcaca ccaccacccc cggctaattt tttgtattta gtagagatgg
     5341 ggtttcacca tgtaggtcag gctggtctcg aactcctgac ctcaggtgat ccacctgcct
     5401 cggcctccca aagacatctc tttatatatt cgaggaactt ggtgtttttg agaaactcgt
     5461 ctaaggagtc tcaacctaat tgccttcctg gaagttagtg aatgagaggc aggaacaagc
     5521 ttccacctct atcttgagag agtcagtgtt ctctcttctt agcactgggc tcatgacaga
     5581 tggatagcag agccagaaac tcacgtggaa ggcaaactgg ccagagaagt catacatgcc
     5641 gcaggaatgc atgaggctga gggtgttgcg cactgcttca gcactcagca cactctcgcc
     5701 tgtgatgggg cagatcccac cgttggcgag ggtggctgcc atgacactgc ctgattcaca
     5761 agtgacctcc acagaacaca gctatgaaaa caaagaatag gtgaagatgt gacgtgaacc
     5821 tgcacatggg accctcgggt gtagggaaag ggcaagggca aggacagtct gtcctgttcc
     5881 ctcggccctg agcagtgttt tcttacctgg aagtagagat caagggcagc catcatgtcc
     5941 acccccttag gaaagcactg caaggaagag aggggagagc atttctcttc aggacatcag
     6001 ccctttcaca ctgtcagggt ctcagctgaa gcccccaacc cctactgccc ttccactagc
     6061 agcagaattc ccagggactt tctcttccgg cctcttctgg ttaccttctt ttccttgaga
     6121 taatagccga tggcataatt ccgatcccct gtttccttct ctgactggaa tctgaagcaa
     6181 acacccaatt tagtacttga agttggagga gtgggtgttg ggagaagggg aaaaattacc
     6241 ttccctggag agacagaact acatcacacc acaaactaaa tgaacccctt tataacttct
     6301 cacagtaata ttagaggaaa tttctgaacc tgagcaaaca ctcagcatag ttttgttata
     6361 aagtaaaccc aaaccaaaca ccccaaactg tctagtcatc taggaaaact ggtgaagttt
     6421 ttggctttga aagtcttggc aagaaagggc ttaggcacaa accatcacac tgcctctctc
     6481 attatgtact aattagaaat ggaacaaatt attttacaag ataaacaaat gtttatgaag
     6541 ggactcagag gaagctggag ggccaaagtt cccctgggat ttcttcctcc taagattctc
     6601 tctcccccat tcctcttgtc cccatcctaa gtacacacac atacaccaca atgctttcag
     6661 tcagtcctct gagtagggac ttacgtggca ttgctgaaac ccatgtattc attcccagcc
     6721 attttgttga gatactgcaa cacctggaag agaaaaaggg acattgaggc tccacttggt
     6781 taagaagctc tattaatacc tcacaggttg actagccctt ctgaactcag gtcttgtcaa
     6841 gaggctttcc tctctgaggc cagcagattt ccacatttct ggaaataagg cttctagtat
     6901 gggctggtgc acctggtagt ggggttagag agccactggg gcagctggag aggacagcat
     6961 gccatggcga aatgcagcca ggggctaagt agcaagggaa cttacaaaat caaacttctc
     7021 tgctttgtta cagtccatct gcagagaaag agagagatgt cagcattcta agtgtaggag
     7081 gatgacagag ggaagggtca gaaggatcta gtggagttct agtgttagtc tggtcctgct
     7141 gctgcaaatg tgttcaattt tataatggcc ctttttttat ccttctgaaa acaccgcact
     7201 tccattggct cctctctttc tctttctttt ttttgagaca gtcttgcttt gtcacccttg
     7261 tcacccaggc tggagtgcag tggcacgatc atggctcact gcagctttga cctcccaggc
     7321 tcaagcgatc ctcccacttc agcctccaag tagctcggat tacaggcatg agccagcttg
     7381 ctgggccatc ccacttccat tctcaatcca aaccctcatc cctgactccc acgttcattt
     7441 tcctgactcg aatcctgtct cctagcccca ccctatccct gatctccatc cccattcctt
     7501 acaccccatg ctggagcttt actccatccc attggccagc atgatggttc atgcctgtaa
     7561 tctcagcaat ttggggggct gaggcaggag gatcgcttga gaccaggggt ttgagaccag
     7621 cctaggcaac ataggagaga ccctgcctca attttttttt tttttttttt ttgagatgga
     7681 gtcttgctct gtcgcccagg ctggagtgca gtggcaggat ctcggctcaa ctgcaagctc
     7741 cgcctcccgg gttcatacca ttctcctgcc ttagcctccc gagcagctgg gaccacaggt
     7801 gcccaccacc aagcctggct aattttttgt atttttagta gagacggggt ttcaccgtgc
     7861 tagccaggat ggtctcgatc tcctgacctc gtgatttgcc cacctcggcc tcccaaagtg
     7921 ttgggattac aggcgtgagc caccgcgcct ggcctctata ttttttaaaa aaggtcctac
     7981 ttagatggga atgggatgag gcaggtccca gatacaggtt tgtagatgga gccatcacag
     8041 aacaagggga gagactcagc ctcttttcct cacccagact cattgcttac tcatgtgtca
     8101 actacagagt caataccaca gggactctga tacctgagtc cctgagggga ggattcctaa
     8161 caccaccttc cccaggaggg gctgtgattg gctccagggt gttgctgccc agctgctccc
     8221 aggttcttgc tcaagccctg ggagacctac agctgatggg ctagtctgac accctgttca
     8281 aggccaactc acagttccag gtgacgtgaa taactgccct ggcctggcca ttcacccttg
     8341 cctgcaggtg tcccacaccc agaagtggcc aatcagtgcc caatagtgct gtcctcccct
     8401 tgtctctggc atttggccct tggtcctagg caggggtcag gacctgctca cttctctaag
     8461 ggtcagaaag ctctaggctc tagactcatg ggtgggggca gggaacagta ctgagtgggg
     8521 atgacggagg tggtgcagtc tcttatactg acattgtcag cataacatgt gggtccctgc
     8581 tgtgcctcat gtgccttctg gggaccctca aaggaatcag agccccacca gtctccctgg
     8641 agagctgaac aaatatgagg gatacaggaa cacaggagct tagaggataa tacctatcag
     8701 aaggttaagg tggcactgac cttgatcagg gagctgacaa caatggcacc agcattgacc
     8761 atggggttat gggggattcc tggggaaata caaaccacca agagtcttag ccactgaaca
     8821 aagcctccct gacagcccgc tcactttgtg ggttagacaa gaaagactgc tagggagaaa
     8881 ggcatgacag ggctaatcag gaagggcaga gaaacaaaaa aggctgggag atggggtggg
     8941 gataaggaga aggggacagc tgtaagtacg gtctgagcct tggtagtgct caccttcctc
     9001 attgagggag agcttgttgt agcgcaggcc acttggctct ttgcccacaa acttgtgcac
     9061 gtagtcagtg cctagggtgc ttatggagat ggcataggtg aggggcttca cacaggactg
     9121 caggcagaag gggatctttg tgtggcccac agagtgcctg gaggcagaca gatgccatga
     9181 aaggggttgg cctgtgttcg gcacctgtgc cctgcctccc cttcctcttg cccagcatct
     9241 caccgttgac catccacagt gcacagggag acaccccaca ggtctgggtt tgacttggcc
     9301 agctgaggga tgtaggctgc cacctggaca tgagtgggta gagaaaaggg agatggatgt
     9361 ggagaagagg aacagagttg aggttgaggg tcaagagaat cttttagata aaagctaaaa
     9421 ttctgtcttc tatagggcag aggggttccc tcctgcctgt tgctcccaga aatgcccgag
     9481 ccttaagtcc tagaaggcca tatctttgtg tatccgcaat cctgtagaga tagcagtaaa
     9541 gccaatggaa gttaaaaaag gagaattctg aggcaacctt ccccttttgg ttcttaggct
     9601 cagttaccct ataaatccct ttgaaggctc tattcgatct tgattccctc acctgctcta
     9661 ggaatcgtgt atagaaacac atgaagccat gtcaccattt acagaagttc tgcttctcat
     9721 ctctcattca taggactccc agccccagga aatctctgag aagcgaggta tatctaggtg
     9781 tggtggctca tgcctgtaat cccagcactt tgggaggccg aggtgggtgg atcacttgag
     9841 atcaggagtt cgagaccagc ctggccaata tggcaaaacc ccatctctac taaaaataca
     9901 aaaattagtc gggcatggtg gtgtatgcca gctactcggg aggctgagac aggagaatcg
     9961 cttgaacctg ggaggtggag gttgcagtga gctgagattg caccattgca ctccagcccg
    10021 ggtgacagag caaaactctg tctcaggaaa aaaaaaaaaa aaaaaaatct ggcccaggtc
    10081 cctgacccct cccttagtcc cctgactctc actttgcctc cagtgagctc tttgacatcc
    10141 tcaaagatgc gatccacatg gcccgtgaac tcctcaaaat caggaatgac aaacttcttt
    10201 cggaatgcct gggtcaggag cacaatgttg ctgctcacac acctggatcc cagacacgat
    10261 tggattaggg ggctagagaa atgcagctgg gactcactct ggagccacgg aaattgggaa
    10321 taaagaaggt tgagtggtct tcaggtttgg gatcaacagc tctgttacct ttggcaaatc
    10381 agtttacctt tctaggtctt agtttccgta cctctaaaat gaggggtttt aatgatgtgg
    10441 aaagacacta tgtcttggga tatgagaaaa aaaataaata ccagaataat atgtatgtat
    10501 gttaccttga tatttaaaaa atcctaaatc ataaaagtat atttatatat attcatgtat
    10561 gtatgtcagt acataggaca ttatctagaa gatacccact aatctgctaa tatttacttc
    10621 tgggaaaaga ggacagggat tgagtaggga gggaaaggag aacatgtatt tctatattgt
    10681 ttgaactctt atgatgagta ttcatgtata acttatgtaa taagtaataa aataaaatga
    10741 ggaattgaac tatacaattc ccaaattcc
//
