LOCUS       NM_001391974            3185 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens shadow of prion protein (SPRN), transcript variant 1,
            mRNA.
ACCESSION   NM_001391974
VERSION     NM_001391974.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Zhang,J., Guo,Y., Xie,W.L., Xu,Y., Ren,K., Shi,Q., Zhang,B.Y.,
            Chen,C., Tian,C., Gao,C. and Dong,X.P.
  TITLE     Disruption of glycosylation enhances ubiquitin-mediated proteasomal
            degradation of Shadoo in Scrapie-infected rodents and cultured
            cells
  JOURNAL   Mol Neurobiol 49 (3), 1373-1384 (2014)
   PUBMED   24390475
  REMARK    GeneRIF: Shadoo is degraded in Scrapie-infected rodents and
            cultured cells mediated by proteasome degradation after
            modification by ubiquitination.
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Sakthivelu,V., Seidel,R.P., Winklhofer,K.F. and Tatzelt,J.
  TITLE     Conserved stress-protective activity between prion protein and
            Shadoo
  JOURNAL   J Biol Chem 286 (11), 8901-8908 (2011)
   PUBMED   21257747
  REMARK    GeneRIF: a conserved physiological activity between PrP(C) and Sho
            to protect cells from stress-induced toxicity and suggests that Sho
            and PrP(C) might act on similar signaling pathways.
REFERENCE   3  (bases 1 to 3185)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   4  (bases 1 to 3185)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   5  (bases 1 to 3185)
  AUTHORS   Beck,J.A., Campbell,T.A., Adamson,G., Poulter,M., Uphill,J.B.,
            Molou,E., Collinge,J. and Mead,S.
  TITLE     Association of a null allele of SPRN with variant Creutzfeldt-Jakob
            disease
  JOURNAL   J Med Genet 45 (12), 813-817 (2008)
   PUBMED   18805828
  REMARK    GeneRIF: Association of a null allele of SPRN with variant
            Creutzfeldt-Jakob disease
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 3185)
  AUTHORS   Van den Brulle,J., Fischer,M., Langmann,T., Horn,G., Waldmann,T.,
            Arnold,S., Fuhrmann,M., Schatz,O., O'Connell,T., O'Connell,D.,
            Auckenthaler,A. and Schwer,H.
  TITLE     A novel solid phase technology for high-throughput gene synthesis
  JOURNAL   Biotechniques 45 (3), 340-343 (2008)
   PUBMED   18778261
  REMARK    GeneRIF: A novel solid phase technology for high- throughput
            synthesis of the SPRN gene is reported.
REFERENCE   7  (bases 1 to 3185)
  AUTHORS   Premzl,M., Gready,J.E., Jermiin,L.S., Simonic,T. and Marshall
            Graves,J.A.
  TITLE     Evolution of vertebrate genes related to prion and Shadoo
            proteins--clues from comparative genomic analysis
  JOURNAL   Mol Biol Evol 21 (12), 2210-2231 (2004)
   PUBMED   15342797
REFERENCE   8  (bases 1 to 3185)
  AUTHORS   Premzl,M., Sangiorgio,L., Strumbo,B., Marshall Graves,J.A.,
            Simonic,T. and Gready,J.E.
  TITLE     Shadoo, a new protein highly conserved from fish to mammals and
            with similarity to prion protein
  JOURNAL   Gene 314, 89-102 (2003)
   PUBMED   14527721
REFERENCE   9  (bases 1 to 3185)
  AUTHORS   Bussow,K., Cahill,D., Nietfeld,W., Bancroft,D., Scherzinger,E.,
            Lehrach,H. and Walter,G.
  TITLE     A method for global protein expression and antibody screening on
            high-density filters of an arrayed cDNA library
  JOURNAL   Nucleic Acids Res 26 (21), 5007-5008 (1998)
   PUBMED   9776767
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL161645.14.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR4352441.320362.1,
                                           ERR4352444.552160.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000685335.1/ ENSP00000510252.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               AL161645.14        9114-9266           c
            154-3185            AL161645.14        5307-8338           c
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.3"
     gene            1..3185
                     /gene="SPRN"
                     /gene_synonym="bA108K14.1; SHADOO; SHO"
                     /note="shadow of prion protein"
                     /db_xref="GeneID:503542"
                     /db_xref="HGNC:HGNC:16871"
                     /db_xref="MIM:610447"
     exon            1..153
                     /gene="SPRN"
                     /gene_synonym="bA108K14.1; SHADOO; SHO"
                     /inference="alignment:Splign:2.1.0"
     exon            154..3185
                     /gene="SPRN"
                     /gene_synonym="bA108K14.1; SHADOO; SHO"
                     /inference="alignment:Splign:2.1.0"
     CDS             170..625
                     /gene="SPRN"
                     /gene_synonym="bA108K14.1; SHADOO; SHO"
                     /note="protein shadoo; hypothetical protein BC004409;
                     shadow of prion protein homolog"
                     /codon_start=1
                     /product="shadow of prion protein precursor"
                     /protein_id="NP_001378903.1"
                     /db_xref="CCDS:CCDS53589.1"
                     /db_xref="GeneID:503542"
                     /db_xref="HGNC:HGNC:16871"
                     /db_xref="MIM:610447"
                     /translation="MNWAPATCWALLLAAAFLCDSGAAKGGRGGARGSARGGVRGGAR
                     GASRVRVRPAQRYGAPGSSLRVAAAGAAAGAAAGAAAGLAAGSGWRRAAGPGERGLED
                     EEDGVPGGNGTGPGIYSYRAWTSGAGPTRGPRLCLVLGGALGALGLLRP"
     regulatory      3166..3171
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRN"
                     /gene_synonym="bA108K14.1; SHADOO; SHO"
                     /note="hexamer: AATAAA"
     polyA_site      3185
                     /gene="SPRN"
                     /gene_synonym="bA108K14.1; SHADOO; SHO"
                     /note="major polyA site"
ORIGIN      
        1 aatcccaggg cggaggcctc gcgcaaaacc caggcgccgc ggctccgcgc tccggctgag
       61 ggtccgcgcc gccgcccgcc cgctccttcc cgctcggccg cggcctcagg gacggatact
      121 ccagcgcgcg gttccaaccg aggcccgtgg cgggtttagc cccacgaaga tgaactgggc
      181 acccgcaacg tgctgggctc tgctactggc ggccgccttc ctctgcgaca gcggcgcagc
      241 caagggcggc cgcggaggtg cgcggggcag tgcccgggga ggggtccgcg ggggtgcgcg
      301 cggggcctcg agggtgcgcg tgaggccggc gcagcgctac ggtgccccgg gctcctccct
      361 gcgcgtggct gccgccgggg cggcagccgg ggcggcggcg ggagcggccg cgggcctggc
      421 ggcgggctcg ggctggagaa gggccgcggg acccggggaa cgcggcctgg aggacgagga
      481 ggacggggtg cccggaggca acgggacagg ccccggcatc tacagctacc gggcgtggac
      541 ttcgggcgct ggacccacgc gcggcccgcg tctctgtctc gtgctgggcg gcgccctcgg
      601 agccctgggg ctgctgcggc cctaggcctg gctgggctcg gggaccacat ctggcccccg
      661 gcccgcgcca tcccccagga tcctccggcc tgggctcccc cttcctccct tgcccacggt
      721 cttggagccc cactgggtgc aggagctgct ggctgtccct gtggacccgc catccaccgt
      781 cctgcccacg ccgcctcagc ctgccacctc ccacctagag gagaccatgg gccctgcccc
      841 acccactcca ggatgttagg gtcccctcag ccaaaaaggc agctgcctgt ggctcctgta
      901 ccaaccgccc agccacgctc catcgccgcc caaagggagg tgccaaggcc aggaacccaa
      961 gccaccccgg ctcccctcgc ctgcccaggg gccgtggtga ctcacgggca gggaggcgac
     1021 atcaggctgg ttctgccact gagcccctga ggaatctgac cctccccaaa agaagcagtg
     1081 aaatggacca aaggacttaa gaatttgggg ggaagtgagg gaaaaacgtt aggtgctaac
     1141 cacctgccca gaagagtgga tctcacagcc caggaacatt cccaagcagg aaaaccgtcc
     1201 gtccgaggaa cgtccatcct ggctctctgc ggctggtggg aaaacacacc ctgccctgag
     1261 gggctgtcca ggccttctcc cccacaccct caggccgaga tccgtgagag acccactttg
     1321 ctccaacaac ttgaaacaag tcactttacc ctccttagga cccattttgg gggggaaaaa
     1381 ccaacacatt ccagagcttt ccaagtcctt tgaacttcag gttcacattc agggatcaca
     1441 cagttctgcc tgttctcagg gcacagcaac tgccaatccc gctgaagagg cctccctggg
     1501 cacagcacag gctgcacggt gcacgcattt ccctgaaggc agccccttct tcggaagcag
     1561 ctgttccagg cctcggaaca gggcctgggt atccgcgtgg tgggctggca gctgacggcc
     1621 tgctcagtgg agccaggagc taactcagac cccaaagcaa gcagggggcc agtggcgggg
     1681 cccagcgccc agcaggacac ccatgcaaga ggctgagccc cccaacatcc aaggacagga
     1741 gagacatgga gtggcgctgg acagtcacga caaggacttg cctccagcac tggacacacc
     1801 tgtgttaaga ccagccctct gcttcccagt cccgccagcc tggggcatcc tccatgggct
     1861 cagcactgag aggtcttggg tctgccacgt tctctagctc tccagtcacc cactcatcca
     1921 gggtaggagg ggttctccct gccccccgcc gtggccttgg gatctcaccc tctccatgtc
     1981 ctggggacag cctcgccctc agccggactg catccctcct gggcctgagc ctcgggactc
     2041 agtggacacc aaagtcaaga ccagcaccca ccacgggccc tgccagcctc tgccttcccc
     2101 agctggcctg ggttctggcc tgggtgagga tctggaagct gttggcagga ctcaaccaag
     2161 cactgctctc tagctccagg gcactaagcc acaggaggca gcgccctgca gcctcccgtc
     2221 cacactgcca gcaatgcccc tggcccagtg agcccagacg ctcctccacc ccttccagac
     2281 caagctcaac gcctccaaga ccagcaggcc aaggccaagc cctgccccag atcctcatag
     2341 gcagagaagc ccttctgaca tttcccccag gaggcagggg gtggtctgag tctcctcaca
     2401 gcagagagac ccaccggagc cccctcaact ttgcagatgc ccacctggaa aatgggctga
     2461 gctgcaccag accctcacac accacagcac tgcaagctga tggaatgttc cagttatgat
     2521 ggacacttcg tgatctgcaa tgactgttga ttcagcacat tagcatctga cacagccaac
     2581 ctgaatactt cctgccccag gcggtcaggg ttatggcacg atgcaggtgg cactcagggg
     2641 ctaacttcag gctgatgagt gtgtggggta tggggcagca gaggcagcca gccagcaaag
     2701 aggggccact gagcaccagg gccctggtgg aggctgctgt gggacggtca ggccaccacc
     2761 gcaaagaggc agccggagct tctgcacagg atgtccctgg ccccaggtcc tgcagcacct
     2821 tagtccatac taccagcccc acccaccttc cttcctcttc cctcttctag gacacaggct
     2881 gtggacccct tcaggtgcac tataatgggg ctggaggggc ccccacatct ctcagcccca
     2941 ctaatgcaga atcccactac ccgtgagcta gaaggtgctc agaggccagg ggtctctact
     3001 gcccatgccg ggcggccttc cagtcattgc acagcaaagc catgtgcagg gcgtccccct
     3061 caaccctgcc ctgaacatgc cccagggcac tgaggggcga agccagtgct tgggctctgc
     3121 tgctgggagt ctctggtctg tgtctgtgtg tgcctgtaag tgtgaaataa acctctctga
     3181 tggca
//
