LOCUS       NM_024738               8426 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens SREBF pathway regulator in golgi 1 (SPRING1),
            transcript variant 1, mRNA.
ACCESSION   NM_024738
VERSION     NM_024738.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Tao,Y., Luo,J., Zhu,H., Chu,Y. and Pei,L.
  TITLE     Chromosome 12 Open Reading Frame 49 Promotes Tumor Growth and
            Predicts Poor Prognosis in Colorectal Cancer
  JOURNAL   Dig Dis Sci 68 (4), 1306-1315 (2023)
   PUBMED   36348128
  REMARK    GeneRIF: Chromosome 12 Open Reading Frame 49 Promotes Tumor Growth
            and Predicts Poor Prognosis in Colorectal Cancer.
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Xiao,J., Xiong,Y., Yang,L.T., Wang,J.Q., Zhou,Z.M., Dong,L.W.,
            Shi,X.J., Zhao,X., Luo,J. and Song,B.L.
  TITLE     POST1/C12ORF49 regulates the SREBP pathway by promoting site-1
            protease maturation
  JOURNAL   Protein Cell 12 (4), 279-296 (2021)
   PUBMED   32666500
  REMARK    GeneRIF: POST1/C12ORF49 regulates the SREBP pathway by promoting
            site-1 protease maturation.
REFERENCE   3  (bases 1 to 8426)
  AUTHORS   Bayraktar,E.C., La,K., Karpman,K., Unlu,G., Ozerdem,C.,
            Ritter,D.J., Alwaseem,H., Molina,H., Hoffmann,H.H., Millner,A.,
            Atilla-Gokcumen,G.E., Gamazon,E.R., Rushing,A.R., Knapik,E.W.,
            Basu,S. and Birsoy,K.
  TITLE     Metabolic coessentiality mapping identifies C12orf49 as a regulator
            of SREBP processing and cholesterol metabolism
  JOURNAL   Nat Metab 2 (6), 487-498 (2020)
   PUBMED   32694732
  REMARK    GeneRIF: Metabolic coessentiality mapping identifies C12orf49 as a
            regulator of SREBP processing and cholesterol metabolism.
REFERENCE   4  (bases 1 to 8426)
  AUTHORS   Aregger,M., Lawson,K.A., Billmann,M., Costanzo,M., Tong,A.H.Y.,
            Chan,K., Rahman,M., Brown,K.R., Ross,C., Usaj,M., Nedyalkova,L.,
            Sizova,O., Habsid,A., Pawling,J., Lin,Z.Y., Abdouni,H., Wong,C.J.,
            Weiss,A., Mero,P., Dennis,J.W., Gingras,A.C., Myers,C.L.,
            Andrews,B.J., Boone,C. and Moffat,J.
  TITLE     Systematic mapping of genetic interactions for de novo fatty acid
            synthesis identifies C12orf49 as a regulator of lipid metabolism
  JOURNAL   Nat Metab 2 (6), 499-513 (2020)
   PUBMED   32694731
  REMARK    GeneRIF: Systematic mapping of genetic interactions for de novo
            fatty acid synthesis identifies C12orf49 as a regulator of lipid
            metabolism.
REFERENCE   5  (bases 1 to 8426)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 8426)
  AUTHORS   Loregger,A., Raaben,M., Nieuwenhuis,J., Tan,J.M.E., Jae,L.T., van
            den Hengel,L.G., Hendrix,S., van den Berg,M., Scheij,S., Song,J.Y.,
            Huijbers,I.J., Kroese,L.J., Ottenhoff,R., van Weeghel,M., van de
            Sluis,B., Brummelkamp,T. and Zelcer,N.
  TITLE     Haploid genetic screens identify SPRING/C12ORF49 as a determinant
            of SREBP signaling and cholesterol metabolism
  JOURNAL   Nat Commun 11 (1), 1128 (2020)
   PUBMED   32111832
  REMARK    GeneRIF: Sterol-regulatory element binding proteins (SREBP)
            regulating gene (SPRING/C12ORF49) is a glycosylated Golgi membrane
            protein and its ablation reduces SREBP signaling. Attenuated SREBP
            signaling in SPRING(KO) cells results from reduced SREBP
            cleavage-activating protein (SCAP) and its mislocalization to Golgi
            irrespective of cellular sterol status. A wide range of tumor cell
            lines display dependency on SPRING expression.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 8426)
  AUTHORS   Wang,Z., Cheng,Y., Abraham,J.M., Yan,R., Liu,X., Chen,W.,
            Ibrahim,S., Schroth,G.P., Ke,X., He,Y. and Meltzer,S.J.
  TITLE     RNA sequencing of esophageal adenocarcinomas identifies novel
            fusion transcripts, including NPC1-MELK, arising from a complex
            chromosomal rearrangement
  JOURNAL   Cancer 123 (20), 3916-3924 (2017)
   PUBMED   28640357
REFERENCE   8  (bases 1 to 8426)
  AUTHORS   Chang,H., Jeung,H.C., Jung,J.J., Kim,T.S., Rha,S.Y. and Chung,H.C.
  TITLE     Identification of genes associated with chemosensitivity to
            SAHA/taxane combination treatment in taxane-resistant breast cancer
            cells
  JOURNAL   Breast Cancer Res Treat 125 (1), 55-63 (2011)
   PUBMED   20224928
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BG537618.1, BC019843.1, AC090013.16, AC125603.7 and BQ006142.1.
            
            On Nov 23, 2018 this sequence version replaced NM_024738.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.120472.1, AK025068.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2147920 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261318.5/ ENSP00000261318.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               BG537618.1         2-140
            140-1261            BC019843.1         1-1122
            1262-2361           AC090013.16        2002-3101           c
            2362-5226           AC125603.7         148247-151111       c
            5227-5277           BQ006142.1         18-68               c
            5278-8426           AC125603.7         145047-148195       c
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.22"
     gene            1..8426
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /note="SREBF pathway regulator in golgi 1"
                     /db_xref="GeneID:79794"
                     /db_xref="HGNC:HGNC:26128"
     exon            1..280
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /inference="alignment:Splign:2.1.0"
     CDS             170..787
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UPF0454 protein C12orf49; partner of site-1 protease;
                     SREBP regulating gene protein"
                     /codon_start=1
                     /product="SREBP regulating gene protein isoform 1"
                     /protein_id="NP_079014.1"
                     /db_xref="CCDS:CCDS9179.1"
                     /db_xref="GeneID:79794"
                     /db_xref="HGNC:HGNC:26128"
                     /translation="MVNLAAMVWRRLLRKRWVLALVFGLSLVYFLSSTFKQEERAVRD
                     RNLLQVHDHNQPIPWKVQFNLGNSSRPSNQCRNSIQGKHLITDELGYVCERKDLLVNG
                     CCNVNVPSTKQYCCDGCWPNGCCSAYEYCVSCCLQPNKQLLLERFLNRAAVAFQNLFM
                     AVEDHFELCLAKCRTSSQSVQHENTYRDPIAKYCYGESPPELFPA"
     exon            281..437
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /inference="alignment:Splign:2.1.0"
     exon            438..589
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /inference="alignment:Splign:2.1.0"
     exon            590..703
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /inference="alignment:Splign:2.1.0"
     exon            704..8426
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1261
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /note="major polyA site"
     polyA_site      5113
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
     polyA_site      5277
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /note="major polyA site"
     regulatory      7474..7479
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /note="hexamer: AATAAA"
     polyA_site      7500
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
     regulatory      7508..7513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /note="hexamer: AGTAAA"
     polyA_site      7526
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
     regulatory      8386..8391
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
                     /note="hexamer: AATAAA"
     polyA_site      8426
                     /gene="SPRING1"
                     /gene_synonym="C12orf49; LUR1; POST1; SPRING"
ORIGIN      
        1 gtcacgtggg gctgcgccca aggctgccgg gagcagactg ggctgcgggc ggcggggccg
       61 gcgcctgcct ggagggatgg ggctgccggg cgcgtagggg ccatgccgcc cgggacccgg
      121 gcctgccgcg ttccgcgccc cggccgccgc gccccacgtc cgcgccggga tggtgaacct
      181 ggcggccatg gtgtggcgcc ggcttctgcg gaagaggtgg gtgctcgccc tggtcttcgg
      241 gctgtcgctc gtctacttcc tcagcagcac cttcaagcag gaggagaggg cagtgagaga
      301 taggaatctc ctccaggttc atgaccataa tcagcccatc ccgtggaaag tgcagtttaa
      361 cttgggcaat agcagtcgtc cgagcaatca gtgccgcaac tccattcaag ggaagcacct
      421 catcacggat gaactcggct acgtttgcga gaggaaggat ttgctggtaa atggctgctg
      481 taatgtcaac gtccctagca cgaagcagta ctgctgtgat ggctgctggc ccaacggctg
      541 ctgcagcgcc tatgagtact gtgtctcctg ctgcctgcag cccaacaagc aacttctcct
      601 ggagcgcttc ctcaaccggg cagccgtggc attccagaac ctcttcatgg cagtcgaaga
      661 tcactttgag ttgtgcctgg ccaaatgcag gacctcatct cagagcgtgc agcatgagaa
      721 cacctaccgg gaccccatag caaagtattg ctatggagaa agcccgcccg agctcttccc
      781 cgcttgacgg gtgcagcgga cttgctccag cctgggtgag gaggccccgc tgaagaactc
      841 gcctcctggg acccagcttc agccatcggg ccaggctgca ggaagaagac aaaggcagcg
      901 tgaggaaacc ttggctttga ccccttctcg tgttgtcatc tttggcttcg ctcaccaccc
      961 gggcttacca gatggaactc ttctgtaaag cagcttggcc cctccagcca gtcccattcg
     1021 ggaaagatga aaccggaggc cgggctcacg gtggtggtgg agttcttgga tgactcagcc
     1081 ctgggacctg cacagggacc tgtgacttgt gttcatcggg ggccggtgtc acttccagtt
     1141 ttgatccagg ctctttcact gtaaaattat ttattggatt cctttggagt aatgggaaca
     1201 ttctaatgtt ttatgtagga aaatgccttg ccattctagt tgaatatgtt caaggaaatt
     1261 atttttgttg ttgttctgtg ttctcgagtt tcaggagtta aatcattctt ccacccagat
     1321 acaacatttt ctcttttagg acgtgaatat tctctctagg cagttatttt tgtttgtatt
     1381 tcgacagtat caagcatagg ccctgaacgt gacctgttag ccatatcctg acgtgtaaaa
     1441 ttatctaaaa actcagacac tcttccattc taatctaact gcacgatttc taacagtggg
     1501 caccatgtgc ctgccctcag gttattttcc agtggtagtc gaatgtgctc atatacccta
     1561 tggagagcac tgttttagca gaaatctaat ttctcttcct ggaggaattt gttctcattt
     1621 cttttgccac ttaaaattaa ctgtgggcta ctcagccagg gtacagtggg agcctcagga
     1681 aggtcagagg caacctcctc ccctgttcta tcaatagaaa cccaacgttg aggcaattcc
     1741 taaacagacg cacctcgtag cttgctgtat gtgtttattc tttattgctt tcagctttgg
     1801 ggctgtaaca ggtacaaata tttggtttcc ctatgattta tagagaagaa gaagaaaccc
     1861 agctttctat cagagcactg caagagaaga gtcttacacc tgccctcagt gggagatgag
     1921 aatggtcatt atgacttaga gaatgctaca cgtgtaggtt gctggtgtgt cctgaatcca
     1981 caggcataaa gcactcccca tttttcctac tgtaatgcag attctccggc tcaaggtcta
     2041 gaatatttga tcctaagatc aagacatcat gcccttcgaa tagtactgct ctttgttttc
     2101 aggagtcacg tgaacacaca actctcctat attcctcacg aacctcagga ttgagcaagg
     2161 tctttgtaat ttttttggtt cactttattg acctgggagc aaggtgctaa ttctgtggtc
     2221 agtattcaat gtttttttca gtggagcttt ttctttgggc catatttgcc ttctaataca
     2281 ttcctgcaat atgtagtggt gatttccctt agcttcctcc tactacctct tatactcatc
     2341 tccccaaatt atttgcctcc cttaaataag ttttcctaga aggtaagctg gtcaggcaat
     2401 ttgaaaaata ttagatccca agaaatctat tccgtttgca ttggacttct cggattccat
     2461 gtgtttgcag caggactaca tcgaactctg atgtgccgga ttgtggcatg tctgcatgtc
     2521 tcatccatct attgtttttg gtaactcagt ttggaatttc agtgtctgtc ttccctgggt
     2581 tgacattgga atcagcctct cctttgagct tattttaact cttgagcaac ataacataga
     2641 tttaatgtga acagtttata ccaaagggca gcctgtgcct gtttatggat cctctctgcc
     2701 tttgtacttg aagagcgcat tttacatttc cagtcctttc acagacagga gctccaacct
     2761 tacgatggag aattaaactt gcttgtattt ccactttgtg gatgaggaac tatgagaggt
     2821 ggagtgactt cctgggtccc cgctgagact tagtgacaga tcccagacaa gaacttcatc
     2881 tctgactcca ggtctagtcc tcttccccct gtctcttgcc aactccagcc ctgacaccgt
     2941 gggcgtctcc cctgagagca gatatatttc aattgtccag gccaaaagag gggcgaggcg
     3001 gcataaacac ccaaattagg tggaggatcc aaaagtcatt ttcatttggc tgtggaatat
     3061 gttttttgta tttcaatcag ctaggggtgt gttcactgtt tttggaaatt cacagcgctt
     3121 gagcctccat aatgaagctg ggctgcagag cacctggcac gtgctccagg ctcccagctc
     3181 ccaacctagg acctctccct gccctccact ctggttggtt tgtggttccc tcgaccgagg
     3241 gtttctagaa tcagggacct tgtctaagtt ttgtttgccc agagcccagc gaagtgtgtg
     3301 atacaccttg ggagtttagg agatcacaaa agggatgaaa acacctttag aaaacatttc
     3361 attggtgggg cgtggtggct tatgcctgta atcccagcac tttggaaggc tgaggcaggt
     3421 ggatcacctg aaggcaggag ttcagaccag cctgaccaat atggtgaaac cccatctata
     3481 ctaaaaatac aaaaattagc cgggtgtggg ggcgtgtgcc tgtttttgtg agactctgtc
     3541 tcaaaaaaaa gaaaaaaata ttgttaataa gtgagtgttt tcggctcaag ggaattgagt
     3601 catacgttgc atctccccca gttctgcatc ttatttgctg cgcttgctgg agttgtaaag
     3661 attggtccta acagggaaag aagcacacaa gagactgtga aaagggctct gagacaagca
     3721 aggacaaaag agggagggaa ataccttcca cagggcctgg gatctggcaa tgctagagcc
     3781 tttttttttt tttttttttt ttgcgatgga gtcttgctct gttgctcagg ctggagtgca
     3841 atggcgtgat ctcagctcac tgcaacctcc ccctcctggg ttcaagcaat tctcctgcct
     3901 cagcctcctg agtagctggg atcacaggcg tacaccacca cacccagcta agtttttgta
     3961 cttttagtag agacggggtt tcaccatgtt ggccaggctg gtcacggact cctgacctca
     4021 gatgatctgc ccgcctgagc ctccgaaagt gctgggatta caggcgtgag ccactgcgcc
     4081 cggcatggag ctgctattga tgggtgagct ccacagcttt tgcagaagca gaggatatga
     4141 cttgagagca gtgctgtcac ctctcagcat gtccccaagc ccaactgggg cctcctggag
     4201 atgcctcagt cggcactggc cccaagggaa tcgtggggaa cagttgcaca atttgcaagt
     4261 ttctgagtgc agcttttccc atccttggga tcagcagata agttgtaaac acagggaggt
     4321 actgcttatt ggatatactt ttcataagta ggacagaatt cttttgggac tctagagttg
     4381 ggaactacca cttactagcg gcgtggctga ggcagtcttc ctcctctgtg gctcaggccc
     4441 ttcatctgtg aaatggggtc acagcatctg cctctcaggg tcactgtgag gtgtcgatgt
     4501 gagcaaggcc tgaggcttgg caagaagtca atgtctgcaa ctcagcaggg agcaatggca
     4561 ggggcagtca ggggtcggct cggatagggg tgggtgggct cctgaggttg gaagggtagg
     4621 aattacagag ctcttgttac tattgttgtt actgttttta aagatacgat atttcagata
     4681 attcaggagc acgtaaggat gaaacttagg ataacctaaa atcacacaac ctagagagaa
     4741 gcgcattttt gtcttccccc attttctagg caaaaatgaa agtactttgt cctcttgaaa
     4801 aacaattctc taatgaatat cctatgttac atagaggcct gtgtaatgca tttgtgtggc
     4861 cacatggtgt caatttcatg aatatacaat aatattatta attccctgct gaggacatta
     4921 actggtttcc aaggctgctt gttgtttttg ctactacaaa taatgcattg atgataaata
     4981 cttttacata catggttgta tgtttatctg aactattttc accaatatat tcacctagtg
     5041 tgtatggaag tgtccatttt tgtcataccc ctggtaaccc tgtgatatta tttttaaaca
     5101 ttttgctaat ggatctctgt tcttgtttga atgtatttaa tttccagcag aatgagcccc
     5161 attccttatt ttgattggcc atttatcatg tacatatggt gaaatgccta ttcgtgactt
     5221 agccaatgtt gtttcttttt cttactgatt actacagtac atttttatat gaaagcagcc
     5281 tttgtcccct tctcttgttt ttcagccctg tgttagaaac aactaagctc acatattcac
     5341 aaaccaactt ttccagttca tctagggcag tggtgctcaa caggggcaat tttgcccccc
     5401 agggaacatt tggcaatgtc tggagacatt tttgtcacag gtggggagga gggcagtact
     5461 gtgggcactc gtggagggag gccagggatg gtgcccaaca gcccccacga cacagaatta
     5521 cctggctgga agtgggagcc gggtggaggg agggaagccc tgctctgggg tcatatctcc
     5581 ccgttagatt gaacagagga gctcctggta gctgagtgct gacttcatta tttttccccg
     5641 cccttgcttt ctaccctgct gaagtgctgc tgattaaatt accctgaaat ccccacagga
     5701 cacattactt cctcacaggt ctctactagc ccagcgctgc ctcaaggaat ggcattattc
     5761 ctttgccact gtgtcctcac cgcactcttg gactccgccg aatgcagaca ccctcctgtc
     5821 tgcagaagga tctggagggg tagaacgctt cccctctacg acggattgac tccccagcac
     5881 ctcaccagca gttctttgaa tggagtggct ttggaggtgg gagttgagga agaaatgtga
     5941 gcctgtcatt acaggttctg tttagaaaac tagtcattct actaaaaagg tatcacataa
     6001 gtaagtagga gcaataaaaa caaagaagat agtgggactc cgagaagctt tccccgcctc
     6061 tggcctgtta gtctatcgtt cgttgggcaa ggctcttcca gtgtgtgacc ttttgcgtgt
     6121 tatcacagca tgagtgcagt tttgtgtaca ggcatcccta tattcagcat gtatctgcaa
     6181 catgccttac ctcgtctgct gtcacagagc aaagtggagc aagtggtcgc cgttggtctg
     6241 cacctgagct gggagccgag tggtgacgtg catatgggcc gactgcgtct ccacagaacc
     6301 tactcagtga tcttcctgtg tacgactcag tccctgatgt tggagttgaa ctgtaggact
     6361 gtctgtaaaa tgaaagtgca gactttaatg tagtaaagag aatgacttgc cagttacaac
     6421 caatgagcaa agcagttaat tattcaaata ttttaccaag agtccaggtt tattttttct
     6481 caggactaca cattctgtga gaccagaggg accaagacat atcactacca gaaatgtgtt
     6541 ttcatcagca ctgccagagg ttttcatagg gtagtttact cttggaattt ttaaattcat
     6601 actttttttt atgcaacgtg gggtaggtaa gcttctgatc actgtgcacc cagattatcc
     6661 caaaatcgaa agtggtgttg gcagccgggt gtggtggctc acacctgtaa tcccagcact
     6721 ttgggaggcc aaggtgagag gattgcttga gcccagaatt cgagaccagc ctgagcaaca
     6781 tagggagacc ctgtctctac aaaaaataaa aaaaaggagc caggcatggt agtgcacacc
     6841 tgtagtccca gctactcagg aggctgaggc gggaggatgg cttgagtcca ggaggtcaag
     6901 gctgcagtga gccgtcattg cgccagtgtg ctccaacctg ggccacagag tgagaccctg
     6961 tctgaggagg ggaaaaaagt gttgtctaaa tgagaaatca ccattatcag taatttaaga
     7021 ttccaggcta tgttggtcac atctcactat tctttttttt ttgaaacgga ttctcgttcc
     7081 atcgcccagg ctggagtgca gtggcacgat ctgggctcgc tgcaagctct gcctcctggg
     7141 ttcacgccat tctcctgcct cagcctcccg agtagctggg actacaggcg cccgccaccg
     7201 tgcccagcta atttttgtat ttttagtaga gacggggttt caccgtgtta gtagccagga
     7261 tggtctcgat ctcctgacct cgtgatccgc ccacctcggc ctcccaaagt gctgggatta
     7321 caggagtgag ccaccgcgcc tggccacatc tcactattct taaggaagag agagagcgag
     7381 agagagtgtt gtcctgtcct gtggggattg gggcattgta ttaagttgta ctgggggatg
     7441 tcaagataga aatgttgagt aagttactac ctgaataaat atttgaccag ttgatgctaa
     7501 tttgaatagt aaatgtgatg acattatata ttctcaggga tgtttccata cattggctct
     7561 gtgtgtgcgt gtgtgtgtgt gtgtgtgtgt aacaaaagca gtatgaagta tgtgagcaat
     7621 atcctaattt tattgacaag agcactgacc ttaatgacct tgagtaaatt accctctccg
     7681 gctttggttt cctcatttgt caattaaggg tgatggctag cgtatcttgc agatccaaat
     7741 tccaattttt gtaacaagcc aaaagtcccc ctggctgtta aagcaagacc agagccctcg
     7801 tatcccagcg gctgggtccc aggcttttga ttttcacagg acaaatgtag cccagccatc
     7861 tggagcccgt ttttctccaa accagggctt cctccttctc tcttgcagat ttcaactggg
     7921 gctcaccatt ttctgcctct tcctcctcca gttttaaaga gacaccatat ccttctctgc
     7981 tgggaccaac ttcccatgta ctgctgccct gcacctgtga aaacagctgc tgtgttttct
     8041 gtcataaaaa tagctgcctt ttggcagaga agcacacaac ggtactacgt ttctgagcct
     8101 taatgcagtg ctcaattatt taggtgtgct tcacctgaga tacacagagc atcttggaac
     8161 tcctactaag cagggcttct acaggcattg ctcagctcag taaaaggttt tcctttctct
     8221 gcttttgtga ttaccaggca gaaaccttaa aatgtatgaa cttacaaaga aattaaattg
     8281 tgccaaatta gattcccagc ccctagagat aaccactaat taaagttccg ctggagcgtt
     8341 ttgcctgtat tttgtatata aacgtggatt ttgtatttgt atgtaaataa aggtaggacc
     8401 ctgctgtcca tattatttta aaataa
//
