LOCUS       NM_181784               4519 bp    mRNA    linear   PRI 23-JUL-2024
DEFINITION  Homo sapiens sprouty related EVH1 domain containing 2 (SPRED2),
            transcript variant 1, mRNA.
ACCESSION   NM_181784
VERSION     NM_181784.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Wang,T., Gao,T., Fujisawa,M., Ohara,T., Sakaguchi,M., Yoshimura,T.
            and Matsukawa,A.
  TITLE     SPRED2 Is a Novel Regulator of Autophagy in Hepatocellular
            Carcinoma Cells and Normal Hepatocytes
  JOURNAL   Int J Mol Sci 25 (11), 6269 (2024)
   PUBMED   38892460
  REMARK    GeneRIF: SPRED2 Is a Novel Regulator of Autophagy in Hepatocellular
            Carcinoma Cells and Normal Hepatocytes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Lopez,J., Bonsor,D.A., Sale,M.J., Urisman,A., Mehalko,J.L.,
            Cabanski-Dunning,M., Castel,P., Simanshu,D.K. and McCormick,F.
  TITLE     The ribosomal S6 kinase 2 (RSK2)-SPRED2 complex regulates the
            phosphorylation of RSK substrates and MAPK signaling
  JOURNAL   J Biol Chem 299 (6), 104789 (2023)
   PUBMED   37149146
  REMARK    GeneRIF: The ribosomal S6 kinase 2 (RSK2)-SPRED2 complex regulates
            the phosphorylation of RSK substrates and MAPK signaling.
REFERENCE   3  (bases 1 to 4519)
  AUTHORS   Wei,J., You,G., Cheng,H. and Gao,C.
  TITLE     SPRED2 promotes autophagy and attenuates inflammatory response in
            IL-1beta induced osteoarthritis chondrocytes via regulating the p38
            MAPK signaling pathway
  JOURNAL   Tissue Cell 82, 102086 (2023)
   PUBMED   37058811
  REMARK    GeneRIF: SPRED2 promotes autophagy and attenuates inflammatory
            response in IL-1beta induced osteoarthritis chondrocytes via
            regulating the p38 MAPK signaling pathway.
REFERENCE   4  (bases 1 to 4519)
  AUTHORS   Gao,T., Yang,X., Fujisawa,M., Ohara,T., Wang,T., Tomonobu,N.,
            Sakaguchi,M., Yoshimura,T. and Matsukawa,A.
  TITLE     SPRED2: A Novel Regulator of Epithelial-Mesenchymal Transition and
            Stemness in Hepatocellular Carcinoma Cells
  JOURNAL   Int J Mol Sci 24 (5), 4996 (2023)
   PUBMED   36902429
  REMARK    GeneRIF: SPRED2: A Novel Regulator of Epithelial-Mesenchymal
            Transition and Stemness in Hepatocellular Carcinoma Cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4519)
  AUTHORS   Markholt,S., Andreasen,L., Bjerre,J., Gregersen,P.A. and
            Andersen,B.N.
  TITLE     Autosomal recessive Noonan-like syndrome caused by homozygosity for
            a previously unreported variant in SPRED2
  JOURNAL   Eur J Med Genet 66 (2), 104695 (2023)
   PUBMED   36608738
  REMARK    GeneRIF: Autosomal recessive Noonan-like syndrome caused by
            homozygosity for a previously unreported variant in SPRED2.
REFERENCE   6  (bases 1 to 4519)
  AUTHORS   Miyoshi,K., Wakioka,T., Nishinakamura,H., Kamio,M., Yang,L.,
            Inoue,M., Hasegawa,M., Yonemitsu,Y., Komiya,S. and Yoshimura,A.
  TITLE     The Sprouty-related protein, Spred, inhibits cell motility,
            metastasis, and Rho-mediated actin reorganization
  JOURNAL   Oncogene 23 (33), 5567-5576 (2004)
   PUBMED   15184877
REFERENCE   7  (bases 1 to 4519)
  AUTHORS   Zimmermann,J., Jarchau,T., Walter,U., Oschkinat,H. and Ball,L.J.
  TITLE     1H, 13C and 15N resonance assignment of the human Spred2 EVH1
            domain
  JOURNAL   J Biomol NMR 29 (3), 435-436 (2004)
   PUBMED   15213456
  REMARK    GeneRIF: NMR assignment of the human Spred2 EVH1 domain
REFERENCE   8  (bases 1 to 4519)
  AUTHORS   Nobuhisa,I., Kato,R., Inoue,H., Takizawa,M., Okita,K., Yoshimura,A.
            and Taga,T.
  TITLE     Spred-2 suppresses aorta-gonad-mesonephros hematopoiesis by
            inhibiting MAP kinase activation
  JOURNAL   J Exp Med 199 (5), 737-742 (2004)
   PUBMED   14981116
REFERENCE   9  (bases 1 to 4519)
  AUTHORS   Kato,R., Nonami,A., Taketomi,T., Wakioka,T., Kuroiwa,A., Matsuda,Y.
            and Yoshimura,A.
  TITLE     Molecular cloning of mammalian Spred-3 which suppresses tyrosine
            kinase-mediated Erk activation
  JOURNAL   Biochem Biophys Res Commun 302 (4), 767-772 (2003)
   PUBMED   12646235
REFERENCE   10 (bases 1 to 4519)
  AUTHORS   Wakioka,T., Sasaki,A., Kato,R., Shouda,T., Matsumoto,A.,
            Miyoshi,K., Tsuneoka,M., Komiya,S., Baron,R. and Yoshimura,A.
  TITLE     Spred is a Sprouty-related suppressor of Ras signalling
  JOURNAL   Nature 412 (6847), 647-651 (2001)
   PUBMED   11493923
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC012370.8, DA102390.1, BC130292.1, AY299090.1 and AK056479.1.
            
            On Nov 23, 2018 this sequence version replaced NM_181784.2.
            
            Summary: SPRED2 is a member of the Sprouty (see SPRY1; MIM
            602465)/SPRED family of proteins that regulate growth
            factor-induced activation of the MAP kinase cascade (see MAPK1; MIM
            176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM,
            Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY299090.1, SRR1803615.263998.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356388.9/ ENSP00000348753.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-77                AC012370.8         65417-65493         c
            78-668              DA102390.1         1-591
            669-3547            BC130292.1         189-3067
            3548-3840           AY299090.1         3132-3424
            3841-4518           AK056479.1         3324-4001
            4519-4519           AY299090.1         4104-4104
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p14"
     gene            1..4519
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /note="sprouty related EVH1 domain containing 2"
                     /db_xref="GeneID:200734"
                     /db_xref="HGNC:HGNC:17722"
                     /db_xref="MIM:609292"
     exon            1..638
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             613..1869
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /note="isoform a is encoded by transcript variant 1;
                     sprouty protein with EVH-1 domain 2, related sequence;
                     sprouty-related, EVH1 domain-containing protein 2"
                     /codon_start=1
                     /product="sprouty-related, EVH1 domain-containing protein
                     2 isoform a"
                     /protein_id="NP_861449.2"
                     /db_xref="CCDS:CCDS33211.1"
                     /db_xref="GeneID:200734"
                     /db_xref="HGNC:HGNC:17722"
                     /db_xref="MIM:609292"
                     /translation="MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVC
                     KVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTF
                     QSPADARAFDRGVRKAIEDLIEGSTTSSSTIHNEAELGDDDVFTTATDSSSNSSQKRE
                     QPTRTISSPTSCEHRRIYTLGHLHDSYPTDHYHLDQPMPRPYRQVSFPDDDEEIVRIN
                     PREKIWMTGYEDYRHAPVRGKYPDPSEDADSSYVRFAKGEVPKHDYNYPYVDSSDFGL
                     GEDPKGRGGSVIKTQPSRGKSRRRKEDGERSRCVYCRDMFNHEENRRGHCQDAPDSVR
                     TCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMC
                     CYLPLRACYHCGVMCRCCGGKHKAAA"
     exon            639..816
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     exon            817..985
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1050
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1200
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..4519
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4500..4505
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /note="hexamer: TATAAA"
     polyA_site      4519
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /note="major polyA site"
ORIGIN      
        1 gcactccacc acctctggcc gctgggaaga cgtcatcgct tccgccctac ttcctcctcc
       61 tgttggcggc ggtgagaatc caatatggag taaatcggtg gagtcgagag atggggctcg
      121 gacggggcgc cctgcaccgc ctcccaggcg cacctccccc ggccgccgac cgctccccgg
      181 aaccgtgatt ggcccggccc cccgggggcg accccggact gagccccccc acccgcggcc
      241 ggcgctctcc tcctcctcgc agcagtccct gccgctccga ggcgcgcttg tttttctcct
      301 gctctccccc accgccgtcc ccttcccaaa tctcgccccc tctgcccgct cccgagcccc
      361 cggagcctcc gcccgcctct ccccacggcg ctgcggcgtt cactccccgc gcagcctgcc
      421 tttgcacccc ttcccccaaa ccctatcccg cgccctgctt ccccttctgc tgcggcgccc
      481 tcttcatctc tagccgcccc ccctccccaa atcaggcgat ctccggagat gtgaagaagg
      541 ggggcgagcg gacaggaaga tgaagggagc aaagctgccc gccgcgggac aggcgtctag
      601 gtgaacaaga aaatgaccga agaaacacac ccagacgatg acagctatat tgtgcgtgtc
      661 aaggctgtgg ttatgaccag agatgactcc agcgggggat ggttcccaca ggaaggaggc
      721 gggatcagtc gcgtcggggt ctgtaaggtc atgcaccccg aaggcaatgg acgaagcggc
      781 tttctcatcc atggtgaacg acagaaagac aaactggtgg tattggaatg ctatgtaaga
      841 aaggacttgg tctacaccaa agccaatcca acgtttcatc actggaaggt cgataatagg
      901 aagtttggac ttactttcca aagccctgct gatgcccgag cctttgacag gggagtaagg
      961 aaagcaatcg aagaccttat agaaggttca acaacgtcat cttccaccat ccataatgaa
     1021 gctgagcttg gcgatgatga cgtttttaca acagctacag acagttcttc taattcctct
     1081 cagaagagag agcaacctac tcggacaatc tcctctccca catcctgtga gcaccggagg
     1141 atttataccc tgggccacct ccacgactca taccccacag accactatca cctcgatcag
     1201 ccgatgccaa ggccctaccg ccaggtgagc ttcccggacg acgacgagga gatcgtgcgc
     1261 atcaaccccc gggagaagat ctggatgacg gggtacgagg attaccggca cgcacccgtc
     1321 aggggcaagt acccggaccc ctcggaggac gcggactcct cctacgtgcg cttcgccaag
     1381 ggcgaggtcc ccaagcatga ctacaactac ccctacgtgg actcctcaga ctttggccta
     1441 ggcgaggacc ccaaaggccg cgggggcagc gtgatcaaga cgcagccctc ccggggcaag
     1501 tcgcggcggc ggaaggagga cggagagcgc tcgcggtgcg tgtactgcag ggacatgttc
     1561 aaccacgagg agaaccgccg gggccactgc caggacgcgc ccgactccgt gagaacttgc
     1621 atccgccggg tgagctgcat gtggtgcgcg gacagcatgc tctatcactg tatgtcggac
     1681 cccgagggag actatacaga cccttgctcg tgcgatacta gcgacgagaa gttttgcctc
     1741 cggtggatgg ctcttattgc cttgtctttc ctggccccct gtatgtgctg ttacctgccc
     1801 cttcgggcct gctaccactg cggagtgatg tgcaggtgct gtggcgggaa gcacaaagcg
     1861 gccgcgtgac tcagtttccc tcccttctcc ctccatccgc agccacaggg gaactcgtct
     1921 cttacatact ctcatcttct cccccgctcc cttccactcc aaggagcgag gagggcaagc
     1981 ggcctcccag ctccctggta cctcgaggca ccattccagc cagggacgct gccgggtaga
     2041 ctctccactc cccctgccgc ccacactgca gcagccacat ccatacacac acgctcgcac
     2101 agtgttctga ggaaggaacc ttcgccacag actcctgtac tattaacaat ctgtaaccaa
     2161 gctaactgtc tcatccatgt gttgatttcc tgtttcctcc tcccccgcct cttccagttc
     2221 aaaggagtct gcaattggaa ctgctgattt tcggtgggtt ttgtagttga tttttccaag
     2281 agcgtcgaag actctctttc tcttggttca ccttgcctgt cgctagcaag catctggttc
     2341 agcggaaatg ggatgtgaga atgatgaaac ccgacagaag tatctcagcc tgcagtcagt
     2401 tattatgtat aggaggtgag ctagttaaca aacttgtacc acaaaacaat atcgctttaa
     2461 cttttctaaa gccaaatttc ccatgtaagc tgcagtttct atctttagcc catcatcatt
     2521 ttctgccccc ccaaaatctg ttgaaatgat tcactgatgc aaaacattca cccgtaatag
     2581 actgagaatt gagccttaac ttcagattaa cttgtgagaa tcaggaaaat tccttcaact
     2641 gcattgcatc ctcttggacc agggctagaa tggggatttc aggtttctat gagcctcccc
     2701 attaccccta aagtagaaca ttttttaaat tgtgttgcca ccacttctaa aaatttagca
     2761 atattagaat aggatactag tgaagtaaga aattttgctt gttgttttgc aaaccaaata
     2821 gtttcctcaa acacaaatca gtgttcccac gaacaagttc cagttgagaa acactaaggt
     2881 tatggtgaat aaaaccatag ggagcttctt ccccccaacc cctggctatt ttatattaat
     2941 ggggagaggg gatttttaaa tgtcataaat ttgaagagtg gtgggttgca ttttcttcat
     3001 gggtttatgt tttcgtttca ttttggactc aatttcacat caccaaattc ctcatttata
     3061 cttggggaaa aaacaaggcc atatgtaaaa acccttccaa tgcctaagtg tctttctcct
     3121 gcaactccaa acccagactc gccactttgg gtgcacaggt ggttaggtca gccaactggt
     3181 tctgcctgtc gccttgccac ggaggaggct tctaattagc tggaaaagag tatttttcta
     3241 atacgttgca aggattagcc aaatcttctt attgaagaaa gaagaaaagt gaagagtggt
     3301 tacctatacc tagcatagta caatcagaac ctcgtggaga ccaccgggga caggcttgcg
     3361 gacgccggct gttcttcccg ccacgatctt tctgtggtag cggccagcag aagacatggc
     3421 ctgctcccca ctccctttcc ccactccttc tttattgcac acaggacacc agtcttcaag
     3481 gaaagggact tttttccagt ctgccaatca tattgggaaa gtgctagctg tgctcacctt
     3541 catggggctg tttccagctc gtccacaagc tcatcgattt tctttagtag attaccggtg
     3601 taaataccca gtgtgcttat gagtcagtta gtagacgtct tcattcattg gagtaactgg
     3661 tttaggcttt ccagtttgga aaaggagcag agagctgtcc atctggattg atggaagaaa
     3721 agagaacctc atccatgcct ggagaacatc tagaaaacct tcagccagcc tccagtgctg
     3781 tcgagagacc accttccccc gacccggagg cacttccttg gggtctttct ctagggtctc
     3841 ttctctacaa agcacaacac taatgttcgt ttccttagac ctcagttcaa gtgcccctat
     3901 ttatttcaat aagaacgcac atatcccagc tgttttttgt ttgtcacctc tatttagttg
     3961 ttacctgttt ctctcttctt tcaccccttg tccttttcca cccttttaag agttacgcta
     4021 gcagatctta ctccacgtat actttttggt ttgtgaaggc atcggttaag ggcacaaaga
     4081 cagccatggg gacatttatg taaatacgtc tctaattgcc acactgcagc tgaacagtgt
     4141 gtagtatttt cccagtcagc tttgccatac tgacgtcaat catttgagag aaattattca
     4201 gattttattt ttgtatctgt ggtaacaaaa cattaaccaa aagattttct gtccagaagc
     4261 ctccccgacc ccccaagcta tttgctcaca ttaacaaatt aaagtgcctg aagcataatt
     4321 cattctttac ctgtatacta aaaaccctgt tgtattgatt tttttataat aagccttttt
     4381 acctctgtgt aaaaaatata tatacaagtg tatgatgtac attttagttc ttaacttttt
     4441 tttatggttt ctaatatgta tgaccaatgt agccattgct ttaaaatgta ccgtgtaaat
     4501 ataaacacat cctatcaga
//
