LOCUS       NM_001128210            4070 bp    mRNA    linear   PRI 23-JUL-2024
DEFINITION  Homo sapiens sprouty related EVH1 domain containing 2 (SPRED2),
            transcript variant 2, mRNA.
ACCESSION   NM_001128210
VERSION     NM_001128210.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Wang,T., Gao,T., Fujisawa,M., Ohara,T., Sakaguchi,M., Yoshimura,T.
            and Matsukawa,A.
  TITLE     SPRED2 Is a Novel Regulator of Autophagy in Hepatocellular
            Carcinoma Cells and Normal Hepatocytes
  JOURNAL   Int J Mol Sci 25 (11), 6269 (2024)
   PUBMED   38892460
  REMARK    GeneRIF: SPRED2 Is a Novel Regulator of Autophagy in Hepatocellular
            Carcinoma Cells and Normal Hepatocytes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Lopez,J., Bonsor,D.A., Sale,M.J., Urisman,A., Mehalko,J.L.,
            Cabanski-Dunning,M., Castel,P., Simanshu,D.K. and McCormick,F.
  TITLE     The ribosomal S6 kinase 2 (RSK2)-SPRED2 complex regulates the
            phosphorylation of RSK substrates and MAPK signaling
  JOURNAL   J Biol Chem 299 (6), 104789 (2023)
   PUBMED   37149146
  REMARK    GeneRIF: The ribosomal S6 kinase 2 (RSK2)-SPRED2 complex regulates
            the phosphorylation of RSK substrates and MAPK signaling.
REFERENCE   3  (bases 1 to 4070)
  AUTHORS   Wei,J., You,G., Cheng,H. and Gao,C.
  TITLE     SPRED2 promotes autophagy and attenuates inflammatory response in
            IL-1beta induced osteoarthritis chondrocytes via regulating the p38
            MAPK signaling pathway
  JOURNAL   Tissue Cell 82, 102086 (2023)
   PUBMED   37058811
  REMARK    GeneRIF: SPRED2 promotes autophagy and attenuates inflammatory
            response in IL-1beta induced osteoarthritis chondrocytes via
            regulating the p38 MAPK signaling pathway.
REFERENCE   4  (bases 1 to 4070)
  AUTHORS   Gao,T., Yang,X., Fujisawa,M., Ohara,T., Wang,T., Tomonobu,N.,
            Sakaguchi,M., Yoshimura,T. and Matsukawa,A.
  TITLE     SPRED2: A Novel Regulator of Epithelial-Mesenchymal Transition and
            Stemness in Hepatocellular Carcinoma Cells
  JOURNAL   Int J Mol Sci 24 (5), 4996 (2023)
   PUBMED   36902429
  REMARK    GeneRIF: SPRED2: A Novel Regulator of Epithelial-Mesenchymal
            Transition and Stemness in Hepatocellular Carcinoma Cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4070)
  AUTHORS   Markholt,S., Andreasen,L., Bjerre,J., Gregersen,P.A. and
            Andersen,B.N.
  TITLE     Autosomal recessive Noonan-like syndrome caused by homozygosity for
            a previously unreported variant in SPRED2
  JOURNAL   Eur J Med Genet 66 (2), 104695 (2023)
   PUBMED   36608738
  REMARK    GeneRIF: Autosomal recessive Noonan-like syndrome caused by
            homozygosity for a previously unreported variant in SPRED2.
REFERENCE   6  (bases 1 to 4070)
  AUTHORS   Miyoshi,K., Wakioka,T., Nishinakamura,H., Kamio,M., Yang,L.,
            Inoue,M., Hasegawa,M., Yonemitsu,Y., Komiya,S. and Yoshimura,A.
  TITLE     The Sprouty-related protein, Spred, inhibits cell motility,
            metastasis, and Rho-mediated actin reorganization
  JOURNAL   Oncogene 23 (33), 5567-5576 (2004)
   PUBMED   15184877
REFERENCE   7  (bases 1 to 4070)
  AUTHORS   Zimmermann,J., Jarchau,T., Walter,U., Oschkinat,H. and Ball,L.J.
  TITLE     1H, 13C and 15N resonance assignment of the human Spred2 EVH1
            domain
  JOURNAL   J Biomol NMR 29 (3), 435-436 (2004)
   PUBMED   15213456
  REMARK    GeneRIF: NMR assignment of the human Spred2 EVH1 domain
REFERENCE   8  (bases 1 to 4070)
  AUTHORS   Nobuhisa,I., Kato,R., Inoue,H., Takizawa,M., Okita,K., Yoshimura,A.
            and Taga,T.
  TITLE     Spred-2 suppresses aorta-gonad-mesonephros hematopoiesis by
            inhibiting MAP kinase activation
  JOURNAL   J Exp Med 199 (5), 737-742 (2004)
   PUBMED   14981116
REFERENCE   9  (bases 1 to 4070)
  AUTHORS   Kato,R., Nonami,A., Taketomi,T., Wakioka,T., Kuroiwa,A., Matsuda,Y.
            and Yoshimura,A.
  TITLE     Molecular cloning of mammalian Spred-3 which suppresses tyrosine
            kinase-mediated Erk activation
  JOURNAL   Biochem Biophys Res Commun 302 (4), 767-772 (2003)
   PUBMED   12646235
REFERENCE   10 (bases 1 to 4070)
  AUTHORS   Wakioka,T., Sasaki,A., Kato,R., Shouda,T., Matsumoto,A.,
            Miyoshi,K., Tsuneoka,M., Komiya,S., Baron,R. and Yoshimura,A.
  TITLE     Spred is a Sprouty-related suppressor of Ras signalling
  JOURNAL   Nature 412 (6847), 647-651 (2001)
   PUBMED   11493923
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC335282.1, BC130292.1, AY299090.1 and AK056479.1.
            
            On May 31, 2019 this sequence version replaced NM_001128210.1.
            
            Summary: SPRED2 is a member of the Sprouty (see SPRY1; MIM
            602465)/SPRED family of proteins that regulate growth
            factor-induced activation of the MAP kinase cascade (see MAPK1; MIM
            176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM,
            Mar 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (b)
            has a shorter and distinct N-terminus compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.69315.1,
                                           SRR1803616.117205.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-551               DC335282.1         33-583
            552-3098            BC130292.1         521-3067
            3099-3391           AY299090.1         3132-3424
            3392-4069           AK056479.1         3324-4001
            4070-4070           AY299090.1         4104-4104
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p14"
     gene            1..4070
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /note="sprouty related EVH1 domain containing 2"
                     /db_xref="GeneID:200734"
                     /db_xref="HGNC:HGNC:17722"
                     /db_xref="MIM:609292"
     exon            1..189
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..1420
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /note="isoform b is encoded by transcript variant 2;
                     sprouty protein with EVH-1 domain 2, related sequence;
                     sprouty-related, EVH1 domain-containing protein 2"
                     /codon_start=1
                     /product="sprouty-related, EVH1 domain-containing protein
                     2 isoform b"
                     /protein_id="NP_001121682.1"
                     /db_xref="CCDS:CCDS46308.1"
                     /db_xref="GeneID:200734"
                     /db_xref="HGNC:HGNC:17722"
                     /db_xref="MIM:609292"
                     /translation="MASPGSDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVM
                     HPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSP
                     ADARAFDRGVRKAIEDLIEGSTTSSSTIHNEAELGDDDVFTTATDSSSNSSQKREQPT
                     RTISSPTSCEHRRIYTLGHLHDSYPTDHYHLDQPMPRPYRQVSFPDDDEEIVRINPRE
                     KIWMTGYEDYRHAPVRGKYPDPSEDADSSYVRFAKGEVPKHDYNYPYVDSSDFGLGED
                     PKGRGGSVIKTQPSRGKSRRRKEDGERSRCVYCRDMFNHEENRRGHCQDAPDSVRTCI
                     RRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYL
                     PLRACYHCGVMCRCCGGKHKAAA"
     exon            190..367
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     exon            368..536
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     exon            537..601
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     exon            602..751
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     exon            752..4070
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4051..4056
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /note="hexamer: TATAAA"
     polyA_site      4070
                     /gene="SPRED2"
                     /gene_synonym="NS14; Spred-2"
                     /note="major polyA site"
ORIGIN      
        1 atccggtaca atgcagtata tttgtcaagg agactgaatc atatatgggg gaaaccactt
       61 ctcaggcagc cagctggttc tcaggtcagc aagagacgat ccatttttat cggaagcttt
      121 atagtagcaa taatgctaat acccagcact cgggctccac aatgtagagg aaatggcatc
      181 gcctggcagt gacagctata ttgtgcgtgt caaggctgtg gttatgacca gagatgactc
      241 cagcggggga tggttcccac aggaaggagg cgggatcagt cgcgtcgggg tctgtaaggt
      301 catgcacccc gaaggcaatg gacgaagcgg ctttctcatc catggtgaac gacagaaaga
      361 caaactggtg gtattggaat gctatgtaag aaaggacttg gtctacacca aagccaatcc
      421 aacgtttcat cactggaagg tcgataatag gaagtttgga cttactttcc aaagccctgc
      481 tgatgcccga gcctttgaca ggggagtaag gaaagcaatc gaagacctta tagaaggttc
      541 aacaacgtca tcttccacca tccataatga agctgagctt ggcgatgatg acgtttttac
      601 aacagctaca gacagttctt ctaattcctc tcagaagaga gagcaaccta ctcggacaat
      661 ctcctctccc acatcctgtg agcaccggag gatttatacc ctgggccacc tccacgactc
      721 ataccccaca gaccactatc acctcgatca gccgatgcca aggccctacc gccaggtgag
      781 cttcccggac gacgacgagg agatcgtgcg catcaacccc cgggagaaga tctggatgac
      841 ggggtacgag gattaccggc acgcacccgt caggggcaag tacccggacc cctcggagga
      901 cgcggactcc tcctacgtgc gcttcgccaa gggcgaggtc cccaagcatg actacaacta
      961 cccctacgtg gactcctcag actttggcct aggcgaggac cccaaaggcc gcgggggcag
     1021 cgtgatcaag acgcagccct cccggggcaa gtcgcggcgg cggaaggagg acggagagcg
     1081 ctcgcggtgc gtgtactgca gggacatgtt caaccacgag gagaaccgcc ggggccactg
     1141 ccaggacgcg cccgactccg tgagaacttg catccgccgg gtgagctgca tgtggtgcgc
     1201 ggacagcatg ctctatcact gtatgtcgga ccccgaggga gactatacag acccttgctc
     1261 gtgcgatact agcgacgaga agttttgcct ccggtggatg gctcttattg ccttgtcttt
     1321 cctggccccc tgtatgtgct gttacctgcc ccttcgggcc tgctaccact gcggagtgat
     1381 gtgcaggtgc tgtggcggga agcacaaagc ggccgcgtga ctcagtttcc ctcccttctc
     1441 cctccatccg cagccacagg ggaactcgtc tcttacatac tctcatcttc tcccccgctc
     1501 ccttccactc caaggagcga ggagggcaag cggcctccca gctccctggt acctcgaggc
     1561 accattccag ccagggacgc tgccgggtag actctccact ccccctgccg cccacactgc
     1621 agcagccaca tccatacaca cacgctcgca cagtgttctg aggaaggaac cttcgccaca
     1681 gactcctgta ctattaacaa tctgtaacca agctaactgt ctcatccatg tgttgatttc
     1741 ctgtttcctc ctcccccgcc tcttccagtt caaaggagtc tgcaattgga actgctgatt
     1801 ttcggtgggt tttgtagttg atttttccaa gagcgtcgaa gactctcttt ctcttggttc
     1861 accttgcctg tcgctagcaa gcatctggtt cagcggaaat gggatgtgag aatgatgaaa
     1921 cccgacagaa gtatctcagc ctgcagtcag ttattatgta taggaggtga gctagttaac
     1981 aaacttgtac cacaaaacaa tatcgcttta acttttctaa agccaaattt cccatgtaag
     2041 ctgcagtttc tatctttagc ccatcatcat tttctgcccc cccaaaatct gttgaaatga
     2101 ttcactgatg caaaacattc acccgtaata gactgagaat tgagccttaa cttcagatta
     2161 acttgtgaga atcaggaaaa ttccttcaac tgcattgcat cctcttggac cagggctaga
     2221 atggggattt caggtttcta tgagcctccc cattacccct aaagtagaac attttttaaa
     2281 ttgtgttgcc accacttcta aaaatttagc aatattagaa taggatacta gtgaagtaag
     2341 aaattttgct tgttgttttg caaaccaaat agtttcctca aacacaaatc agtgttccca
     2401 cgaacaagtt ccagttgaga aacactaagg ttatggtgaa taaaaccata gggagcttct
     2461 tccccccaac ccctggctat tttatattaa tggggagagg ggatttttaa atgtcataaa
     2521 tttgaagagt ggtgggttgc attttcttca tgggtttatg ttttcgtttc attttggact
     2581 caatttcaca tcaccaaatt cctcatttat acttggggaa aaaacaaggc catatgtaaa
     2641 aacccttcca atgcctaagt gtctttctcc tgcaactcca aacccagact cgccactttg
     2701 ggtgcacagg tggttaggtc agccaactgg ttctgcctgt cgccttgcca cggaggaggc
     2761 ttctaattag ctggaaaaga gtatttttct aatacgttgc aaggattagc caaatcttct
     2821 tattgaagaa agaagaaaag tgaagagtgg ttacctatac ctagcatagt acaatcagaa
     2881 cctcgtggag accaccgggg acaggcttgc ggacgccggc tgttcttccc gccacgatct
     2941 ttctgtggta gcggccagca gaagacatgg cctgctcccc actccctttc cccactcctt
     3001 ctttattgca cacaggacac cagtcttcaa ggaaagggac ttttttccag tctgccaatc
     3061 atattgggaa agtgctagct gtgctcacct tcatggggct gtttccagct cgtccacaag
     3121 ctcatcgatt ttctttagta gattaccggt gtaaataccc agtgtgctta tgagtcagtt
     3181 agtagacgtc ttcattcatt ggagtaactg gtttaggctt tccagtttgg aaaaggagca
     3241 gagagctgtc catctggatt gatggaagaa aagagaacct catccatgcc tggagaacat
     3301 ctagaaaacc ttcagccagc ctccagtgct gtcgagagac caccttcccc cgacccggag
     3361 gcacttcctt ggggtctttc tctagggtct cttctctaca aagcacaaca ctaatgttcg
     3421 tttccttaga cctcagttca agtgccccta tttatttcaa taagaacgca catatcccag
     3481 ctgttttttg tttgtcacct ctatttagtt gttacctgtt tctctcttct ttcacccctt
     3541 gtccttttcc acccttttaa gagttacgct agcagatctt actccacgta tactttttgg
     3601 tttgtgaagg catcggttaa gggcacaaag acagccatgg ggacatttat gtaaatacgt
     3661 ctctaattgc cacactgcag ctgaacagtg tgtagtattt tcccagtcag ctttgccata
     3721 ctgacgtcaa tcatttgaga gaaattattc agattttatt tttgtatctg tggtaacaaa
     3781 acattaacca aaagattttc tgtccagaag cctccccgac cccccaagct atttgctcac
     3841 attaacaaat taaagtgcct gaagcataat tcattcttta cctgtatact aaaaaccctg
     3901 ttgtattgat ttttttataa taagcctttt tacctctgtg taaaaaatat atatacaagt
     3961 gtatgatgta cattttagtt cttaactttt ttttatggtt tctaatatgt atgaccaatg
     4021 tagccattgc tttaaaatgt accgtgtaaa tataaacaca tcctatcaga
//
