LOCUS       NM_152988               3691 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens signal peptide peptidase like 2B (SPPL2B), transcript
            variant 2, mRNA.
ACCESSION   NM_152988 NM_020172
VERSION     NM_152988.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Maccioni,R., Travisan,C., Badman,J., Zerial,S., Wagener,A.,
            Andrade-Talavera,Y., Picciau,F., Grassi,C., Chen,G., Lemoine,L.,
            Fisahn,A., Jiang,R., Fluhrer,R., Mentrup,T., Schroder,B.,
            Nilsson,P. and Tambaro,S.
  TITLE     Signal peptide peptidase-like 2b modulates the amyloidogenic
            pathway and exhibits an Abeta-dependent expression in Alzheimer's
            disease
  JOURNAL   Prog Neurobiol 235, 102585 (2024)
   PUBMED   38367747
  REMARK    GeneRIF: Signal peptide peptidase-like 2b modulates the
            amyloidogenic pathway and exhibits an Abeta-dependent expression in
            Alzheimer's disease.
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 3691)
  AUTHORS   Mentrup,T., Theodorou,K., Cabrera-Cabrera,F., Helbig,A.O., Happ,K.,
            Gijbels,M., Gradtke,A.C., Rabe,B., Fukumori,A., Steiner,H.,
            Tholey,A., Fluhrer,R., Donners,M. and Schroder,B.
  TITLE     Atherogenic LOX-1 signaling is controlled by SPPL2-mediated
            intramembrane proteolysis
  JOURNAL   J Exp Med 216 (4), 807-830 (2019)
   PUBMED   30819724
  REMARK    GeneRIF: SPPL2a/b negatively regulates LOX-1 signaling and
            atherosclerosis.
REFERENCE   4  (bases 1 to 3691)
  AUTHORS   Nalls,M.A., Pankratz,N., Lill,C.M., Do,C.B., Hernandez,D.G.,
            Saad,M., DeStefano,A.L., Kara,E., Bras,J., Sharma,M., Schulte,C.,
            Keller,M.F., Arepalli,S., Letson,C., Edsall,C., Stefansson,H.,
            Liu,X., Pliner,H., Lee,J.H., Cheng,R., Ikram,M.A., Ioannidis,J.P.,
            Hadjigeorgiou,G.M., Bis,J.C., Martinez,M., Perlmutter,J.S.,
            Goate,A., Marder,K., Fiske,B., Sutherland,M., Xiromerisiou,G.,
            Myers,R.H., Clark,L.N., Stefansson,K., Hardy,J.A., Heutink,P.,
            Chen,H., Wood,N.W., Houlden,H., Payami,H., Brice,A., Scott,W.K.,
            Gasser,T., Bertram,L., Eriksson,N., Foroud,T. and Singleton,A.B.
  CONSRTM   International Parkinson's Disease Genomics Consortium (IPDGC);
            Parkinson's Study Group (PSG) Parkinson's Research: The Organized
            GENetics Initiative (PROGENI); 23andMe; GenePD; NeuroGenetics
            Research Consortium (NGRC); Hussman Institute of Human Genomics
            (HIHG); Ashkenazi Jewish Dataset Investigator; Cohorts for Health
            and Aging Research in Genetic Epidemiology (CHARGE); North American
            Brain Expression Consortium (NABEC); United Kingdom Brain
            Expression Consortium (UKBEC); Greek Parkinson's Disease
            Consortium; Alzheimer Genetic Analysis Group
  TITLE     Large-scale meta-analysis of genome-wide association data
            identifies six new risk loci for Parkinson's disease
  JOURNAL   Nat Genet 46 (9), 989-993 (2014)
   PUBMED   25064009
REFERENCE   5  (bases 1 to 3691)
  AUTHORS   Zahn,C., Kaup,M., Fluhrer,R. and Fuchs,H.
  TITLE     The transferrin receptor-1 membrane stub undergoes intramembrane
            proteolysis by signal peptide peptidase-like 2b
  JOURNAL   FEBS J 280 (7), 1653-1663 (2013)
   PUBMED   23384347
  REMARK    GeneRIF: TfR1 N-terminal fragment is a substrate for intramembrane
            proteolysis by SPPL2b.
REFERENCE   6  (bases 1 to 3691)
  AUTHORS   Friedmann,E., Lemberg,M.K., Weihofen,A., Dev,K.K., Dengler,U.,
            Rovelli,G. and Martoglio,B.
  TITLE     Consensus analysis of signal peptide peptidase and homologous human
            aspartic proteases reveals opposite topology of catalytic domains
            compared with presenilins
  JOURNAL   J Biol Chem 279 (49), 50790-50798 (2004)
   PUBMED   15385547
  REMARK    GeneRIF: SPP, SPPL2a, -2b, -2c, and -3 probably cleave type
            II-oriented substrate peptides as shown by consensus analysis
REFERENCE   7  (bases 1 to 3691)
  AUTHORS   Grimwood,J., Gordon,L.A., Olsen,A., Terry,A., Schmutz,J.,
            Lamerdin,J., Hellsten,U., Goodstein,D., Couronne,O.,
            Tran-Gyamfi,M., Aerts,A., Altherr,M., Ashworth,L., Bajorek,E.,
            Black,S., Branscomb,E., Caenepeel,S., Carrano,A., Caoile,C.,
            Chan,Y.M., Christensen,M., Cleland,C.A., Copeland,A., Dalin,E.,
            Dehal,P., Denys,M., Detter,J.C., Escobar,J., Flowers,D.,
            Fotopulos,D., Garcia,C., Georgescu,A.M., Glavina,T., Gomez,M.,
            Gonzales,E., Groza,M., Hammon,N., Hawkins,T., Haydu,L., Ho,I.,
            Huang,W., Israni,S., Jett,J., Kadner,K., Kimball,H., Kobayashi,A.,
            Larionov,V., Leem,S.H., Lopez,F., Lou,Y., Lowry,S., Malfatti,S.,
            Martinez,D., McCready,P., Medina,C., Morgan,J., Nelson,K.,
            Nolan,M., Ovcharenko,I., Pitluck,S., Pollard,M., Popkie,A.P.,
            Predki,P., Quan,G., Ramirez,L., Rash,S., Retterer,J., Rodriguez,A.,
            Rogers,S., Salamov,A., Salazar,A., She,X., Smith,D., Slezak,T.,
            Solovyev,V., Thayer,N., Tice,H., Tsai,M., Ustaszewska,A., Vo,N.,
            Wagner,M., Wheeler,J., Wu,K., Xie,G., Yang,J., Dubchak,I.,
            Furey,T.S., DeJong,P., Dickson,M., Gordon,D., Eichler,E.E.,
            Pennacchio,L.A., Richardson,P., Stubbs,L., Rokhsar,D.S.,
            Myers,R.M., Rubin,E.M. and Lucas,S.M.
  TITLE     The DNA sequence and biology of human chromosome 19
  JOURNAL   Nature 428 (6982), 529-535 (2004)
   PUBMED   15057824
REFERENCE   8  (bases 1 to 3691)
  AUTHORS   Grigorenko,A.P., Moliaka,Y.K., Korovaitseva,G.I. and Rogaev,E.I.
  TITLE     Novel class of polytopic proteins with domains associated with
            putative protease activity
  JOURNAL   Biochemistry (Mosc) 67 (7), 826-835 (2002)
   PUBMED   12139484
REFERENCE   9  (bases 1 to 3691)
  AUTHORS   Weihofen,A., Binns,K., Lemberg,M.K., Ashman,K. and Martoglio,B.
  TITLE     Identification of signal peptide peptidase, a presenilin-type
            aspartic protease
  JOURNAL   Science 296 (5576), 2215-2218 (2002)
   PUBMED   12077416
REFERENCE   10 (bases 1 to 3691)
  AUTHORS   Petroff,O.A., Novotny,E.J., Ogino,T., Avison,M. and Prichard,J.W.
  TITLE     In vivo measurements of ethanol concentration in rabbit brain by 1H
            magnetic resonance spectroscopy
  JOURNAL   J Neurochem 54 (4), 1188-1195 (1990)
   PUBMED   2313285
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC093046.1, AB040965.1 and
            BU733058.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_152988.2.
            
            Summary: This gene encodes a member of the GXGD family of aspartic
            proteases. The GXGD proteases are transmembrane proteins with two
            conserved catalytic motifs localized within the membrane-spanning
            regions. This enzyme localizes to endosomes, lysosomes, and the
            plasma membrane. It cleaves the transmembrane domain of tumor
            necrosis factor alpha to release the intracellular domain, which
            triggers cytokine expression in the innate and adaptive immunity
            pathways. Multiple transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) represents the shorter
            transcript but encodes the longer isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB040965.1, BC093046.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000613503.5/ ENSP00000478298.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-751               BC093046.1         55-805
            752-3397            AB040965.1         755-3400
            3398-3691           BU733058.1         25-318              c
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..3691
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /note="signal peptide peptidase like 2B"
                     /db_xref="GeneID:56928"
                     /db_xref="HGNC:HGNC:30627"
                     /db_xref="MIM:608239"
     exon            1..92
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..1805
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /EC_number="3.4.23.-"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; SPP-like 2B; intramembrane protease 4;
                     presenilin-like protein 1; presenilin homologous protein
                     4"
                     /codon_start=1
                     /product="signal peptide peptidase-like 2B isoform 2
                     precursor"
                     /protein_id="NP_694533.1"
                     /db_xref="CCDS:CCDS74252.1"
                     /db_xref="GeneID:56928"
                     /db_xref="HGNC:HGNC:30627"
                     /db_xref="MIM:608239"
                     /translation="MAAAVAAALARLLAAFLLLAAQVACEYGMVHVVSQAGGPEGKDY
                     CILYNPQWAHLPHDLSKASFLQLRNWTASLLCSAADLPARGFSNQIPLVARGNCTFYE
                     KVRLAQGSGARGLLIVSRERLVPPGGNKTQYDEIGIPVALLSYKDMLDIFTRFGRTVR
                     AALYAPKEPVLDYNMVIIFIMAVGTVAIGGYWAGSRDVKKRYMKHKRDDGPEKQEDEA
                     VDVTPVMTCVFVVMCCSMLVLLYYFYDLLVYVVIGIFCLASATGLYSCLAPCVRRLPF
                     GKCRIPNNSLPYFHKRPQARMLLLALFCVAVSVVWGVFRNEDQWAWVLQDALGIAFCL
                     YMLKTIRLPTFKACTLLLLVLFLYDIFFVFITPFLTKSGSSIMVEVATGPSDSATREK
                     LPMVLKVPRLNSSPLALCDRPFSLLGFGDILVPGLLVAYCHRFDIQVQSSRVYFVACT
                     IAYGVGLLVTFVALALMQRGQPALLYLVPCTLVTSCAVALWRRELGVFWTGSGFAKVL
                     PPSPWAPAPADGPQPPKDSATPLSPQPPSEEPATSPWPAEQSPKSRTSEEMGAGAPMR
                     EPGSPAESEGRDQAQPSPVTQPGASA"
     exon            93..212
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            213..395
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            396..485
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            486..625
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            626..768
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            769..865
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            866..982
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1064
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1139
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1202
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1302
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1380
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1541
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..3691
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2506..2511
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /note="hexamer: AATAAA"
     polyA_site      2529
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /note="major polyA site"
     regulatory      3377..3382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /note="hexamer: ATTAAA"
     polyA_site      3400
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /note="major polyA site"
     regulatory      3674..3679
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
                     /note="hexamer: ATTAAA"
     polyA_site      3691
                     /gene="SPPL2B"
                     /gene_synonym="IMP-4; IMP4; PSH4; PSL1"
ORIGIN      
        1 gttggttgcg gcgggcaccg gccgacatgg cggcagcggt ggcggctgcg ctggcgcggc
       61 ttttggcggc ctttctgctc ctcgcggccc aggtggcctg tgagtacggc atggtgcacg
      121 tggtctccca ggccgggggc cccgaaggca aagactactg catcctctac aacccgcagt
      181 gggcccatct tccgcacgac ctcagcaagg catctttcct gcagctgcgc aactggacgg
      241 cctccctgct ctgctccgca gccgacctcc ccgcccgtgg cttcagcaac cagatcccgc
      301 tggtggcgcg ggggaactgc accttctatg agaaagtgag gctggcccag ggcagcggag
      361 cacgcgggct gctcatcgtc agcagggaga ggctggtccc cccggggggt aataagacgc
      421 agtatgatga gattggcatt cccgtggccc tgctcagcta caaagacatg ctggacatct
      481 tcacgcgttt cggccgcacg gtgagggcgg cgctgtatgc gcctaaggag ccggtgctgg
      541 actacaacat ggtcatcatc ttcatcatgg ctgtgggcac cgtcgccatc ggcggctact
      601 gggccgggag tcgggacgtg aagaaaaggt acatgaagca caagcgcgac gatgggcccg
      661 agaagcagga ggacgaggcg gtggacgtga cgccggtgat gacctgcgtg tttgtggtga
      721 tgtgctgctc catgctggtg ctgctctact atttctacga tctcctcgtg tacgtggtca
      781 tcgggatctt ctgcctggcc tccgccaccg gcctctacag ctgcctggcg ccctgtgtgc
      841 ggcggctgcc cttcggcaag tgcaggatcc ccaacaacag cctgccctac ttccacaagc
      901 gcccgcaggc ccgtatgctg ctcctggcgc tcttctgcgt ggccgtcagc gtggtgtggg
      961 gcgtcttccg caacgaggac cagtgggcct gggtcctcca ggatgccctg ggcatcgcct
     1021 tctgcctcta catgctgaag accatccgtc tgcccacctt caaggcctgc acgctgctgc
     1081 tgctggtgct gttcctctac gacatcttct tcgtgttcat cacgcccttc ctgaccaaga
     1141 gtgggagcag catcatggtg gaggtggcca ctgggccctc ggactcagcc acccgtgaga
     1201 agctgcccat ggtcctgaag gtgcccaggc tgaactcctc acctctggcc ctgtgtgacc
     1261 ggcccttctc cctcctgggt ttcggagaca ttttggtgcc agggctgctg gtggcctact
     1321 gccacaggtt tgacatccag gtacagtcct ccagggtata cttcgtggcc tgcaccatcg
     1381 cctatggcgt tggcctcctt gtgacattcg tggcactggc cctgatgcag cgtggccagc
     1441 ccgctctcct ctacctggtg ccctgcacgc tggtgacgag ctgcgctgtg gcgctctggc
     1501 gccgggagct gggcgtgttc tggacgggca gcggctttgc gaaagtccta cctccatctc
     1561 cgtgggcccc agcaccagcc gacggcccgc agcctcccaa agactctgcc acgccactct
     1621 ccccgcagcc gcccagcgaa gaaccagcca catccccctg gcctgctgag cagtccccaa
     1681 aatcacgcac gtccgaggag atgggggctg gagcccccat gcgggagcct gggagcccag
     1741 ctgaatccga gggccgggac caggcccagc cgtccccggt aacccagcct ggcgcctcgg
     1801 cctaggggag gggtgagacg ctcgctgccg tgcccgccac accaagatgt tggggctgcc
     1861 tggcgcccac tggagacaga cagacagacg cctgtccccc gggaccgagg cctgtgccgt
     1921 ccccacccgc cccaacatgg tgctcatcct tgccgagacc cctgcggtct gtgcccgcgc
     1981 ccagcccagc tgccccggct gcacgcctgc tgctcccagc tcgcccggct gccacaagct
     2041 ctctgcgggt ccatcctccc caccggggtc cgtcctcgca ggccctgccc ggcctctctg
     2101 cagaccctca agcgtcgtct gcatgagtga gcaggcgtgg gtggactctg gccgcggcca
     2161 cacttggtgc tcaccagctg cttcggcctt caggtgacct ccctccccac ggcatcctgc
     2221 tctccgggtg gaagagcagc tttctgtctc ccagaaggca tcgcttttcc ctcttgagca
     2281 gatcggagcc cctgggaggt ttggaagctg cctccaagcc taggacacgg accagtggcc
     2341 ggggcggcct ctggcccctg acgctggctg agacaggccc gtggggcggg gttttggggc
     2401 gtgaacaagg ctggcagtaa gtggacaagc tgctcccctg gctaaggccc tgccctgccc
     2461 tcagccagag gtgcctggcc atgcctgcac actcctcccc attttaataa atggtcgcaa
     2521 cttctagaag tcctgctggt gccacctggt ggggtggacg tctctcagac tgcccttctg
     2581 acaagcaggg gtgggcgcca ggcaggctgg gtcaggccct caggggtccc tggagccctg
     2641 ggagggaggg actgggtcgt gggaggtcct ggtagctccc ggcacccaac ctcgcttccc
     2701 gtgtgggccc cgtgttgctt ttctgctgag aggggcttgg gcctcggttc tccctgtggc
     2761 agcggcattg gtgcctgggt tcttaaccct ctggacccag cagctaggag cttctggaac
     2821 ccacgaggac atctgccact ggcatggtct actcactgat ggggtcctcc tgactccagg
     2881 gcagagacgt ccgtgcggga actgaccgag gcgtggcccc aggccccggc atcctcccct
     2941 cgctgcacgt ggtcacttcc gaagcagcgc tccctgtggc cgcagagaca gagcccgcac
     3001 cctggctgtc tgtggtgcct gggccccagc tcccgcccgc cacccgccct gttgagtgcg
     3061 gcctccagac tcgagtccag aatctgtttc tgtcaagtca gtcgtcccca gctgcacacg
     3121 gggactgctg agcgctgcgc tctcacgggt ctgttccgga cagagggtgg caggggtgag
     3181 ccctgatccc ttacaaaaaa taccagcttc ccagggagta atggcgcctt cactgaagcc
     3241 attttgtaca tggggacggg gtggggacgt ctcttttttg gtgttggacg tttgaccagt
     3301 ggggaggggt tcagaggcgg cggtcctgtc cttggctccg tctgtccgtg tggccttgct
     3361 gccattccgt ctgatcatta aacacagctt ttgatacata tggcccggcc tctgcctccg
     3421 cagtgcacgg cttggggcat ctgctctgtg caggtcccgg tgagcagaac tgagagctgg
     3481 caggccgcct ggctgtcctg cagaggacgg attggaattg ccaccgcagg gccggccagt
     3541 gctgtgaggt gcccagcgtc actcccatgg gctctgtggg ccatgtggtc cctgtcacgc
     3601 ttctcagctc tgccactgtt gctggaaagc atcacagacc atgtgtaaaa tgggccaaaa
     3661 taccttttac aaaattaaaa tagagatggg g
//
