LOCUS       NM_032802               7270 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens signal peptide peptidase like 2A (SPPL2A), mRNA.
ACCESSION   NM_032802
VERSION     NM_032802.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 7270)
  AUTHORS   Mentrup,T., Theodorou,K., Cabrera-Cabrera,F., Helbig,A.O., Happ,K.,
            Gijbels,M., Gradtke,A.C., Rabe,B., Fukumori,A., Steiner,H.,
            Tholey,A., Fluhrer,R., Donners,M. and Schroder,B.
  TITLE     Atherogenic LOX-1 signaling is controlled by SPPL2-mediated
            intramembrane proteolysis
  JOURNAL   J Exp Med 216 (4), 807-830 (2019)
   PUBMED   30819724
  REMARK    GeneRIF: SPPL2a/b negatively regulates LOX-1 signaling and
            atherosclerosis.
REFERENCE   4  (bases 1 to 7270)
  AUTHORS   Kong,X.F., Martinez-Barricarte,R., Kennedy,J., Mele,F., Lazarov,T.,
            Deenick,E.K., Ma,C.S., Breton,G., Lucero,K.B., Langlais,D.,
            Bousfiha,A., Aytekin,C., Markle,J., Trouillet,C., Jabot-Hanin,F.,
            Arlehamn,C.S.L., Rao,G., Picard,C., Lasseau,T., Latorre,D.,
            Hambleton,S., Deswarte,C., Itan,Y., Abarca,K.,
            Moraes-Vasconcelos,D., Ailal,F., Ikinciogullari,A., Dogu,F.,
            Benhsaien,I., Sette,A., Abel,L., Boisson-Dupuis,S., Schroder,B.,
            Nussenzweig,M.C., Liu,K., Geissmann,F., Tangye,S.G., Gros,P.,
            Sallusto,F., Bustamante,J. and Casanova,J.L.
  TITLE     Disruption of an antimycobacterial circuit between dendritic and
            helper T cells in human SPPL2a deficiency
  JOURNAL   Nat Immunol 19 (9), 973-985 (2018)
   PUBMED   30127434
  REMARK    GeneRIF: inherited SPPL2a deficiency in humans underlies
            mycobacterial disease by decreasing the numbers of dendritic cells
            and impairing IFN-gamma production by mycobacterium-specific memory
            TH1* cells
REFERENCE   5  (bases 1 to 7270)
  AUTHORS   Huttl,S., Helfrich,F., Mentrup,T., Held,S., Fukumori,A.,
            Steiner,H., Saftig,P., Fluhrer,R. and Schroder,B.
  TITLE     Substrate determinants of signal peptide peptidase-like 2a
            (SPPL2a)-mediated intramembrane proteolysis of the invariant chain
            CD74
  JOURNAL   Biochem J 473 (10), 1405-1422 (2016)
   PUBMED   26987812
  REMARK    GeneRIF: Study propose that elements within the transmembrane
            segment and the luminal juxtamembrane domain facilitate
            intramembrane proteolysis of CD74 by SPPL2a.
REFERENCE   6  (bases 1 to 7270)
  AUTHORS   Fluhrer,R., Grammer,G., Israel,L., Condron,M.M., Haffner,C.,
            Friedmann,E., Bohland,C., Imhof,A., Martoglio,B., Teplow,D.B. and
            Haass,C.
  TITLE     A gamma-secretase-like intramembrane cleavage of TNFalpha by the
            GxGD aspartyl protease SPPL2b
  JOURNAL   Nat Cell Biol 8 (8), 894-896 (2006)
   PUBMED   16829951
REFERENCE   7  (bases 1 to 7270)
  AUTHORS   Friedmann,E., Lemberg,M.K., Weihofen,A., Dev,K.K., Dengler,U.,
            Rovelli,G. and Martoglio,B.
  TITLE     Consensus analysis of signal peptide peptidase and homologous human
            aspartic proteases reveals opposite topology of catalytic domains
            compared with presenilins
  JOURNAL   J Biol Chem 279 (49), 50790-50798 (2004)
   PUBMED   15385547
  REMARK    GeneRIF: SPP, SPPL2a, -2b, -2c, and -3 probably cleave type
            II-oriented substrate peptides as shown by consensus analysis
REFERENCE   8  (bases 1 to 7270)
  AUTHORS   Grigorenko,A.P., Moliaka,Y.K., Korovaitseva,G.I. and Rogaev,E.I.
  TITLE     Novel class of polytopic proteins with domains associated with
            putative protease activity
  JOURNAL   Biochemistry (Mosc) 67 (7), 826-835 (2002)
   PUBMED   12139484
REFERENCE   9  (bases 1 to 7270)
  AUTHORS   Weihofen,A., Binns,K., Lemberg,M.K., Ashman,K. and Martoglio,B.
  TITLE     Identification of signal peptide peptidase, a presenilin-type
            aspartic protease
  JOURNAL   Science 296 (5576), 2215-2218 (2002)
   PUBMED   12077416
REFERENCE   10 (bases 1 to 7270)
  AUTHORS   Petroff,O.A., Novotny,E.J., Ogino,T., Avison,M. and Prichard,J.W.
  TITLE     In vivo measurements of ethanol concentration in rabbit brain by 1H
            magnetic resonance spectroscopy
  JOURNAL   J Neurochem 54 (4), 1188-1195 (1990)
   PUBMED   2313285
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY169314.1, AK027446.1,
            AA988423.1 and AC012100.7.
            
            On Nov 22, 2018 this sequence version replaced NM_032802.3.
            
            Summary: This gene encodes a member of the GXGD family of aspartic
            proteases, which are transmembrane proteins with two conserved
            catalytic motifs localized within the membrane-spanning regions, as
            well as a member of the signal peptide peptidase-like protease
            (SPPL) family. This protein is expressed in all major adult human
            tissues and localizes to late endosomal compartments and lysosomal
            membranes. A pseudogene of this gene also lies on chromosome 15.
            [provided by RefSeq, Feb 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.119776.1,
                                           SRR1660803.160277.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261854.10/ ENSP00000261854.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1824              AY169314.1         4-1827
            1825-1994           AK027446.1         1384-1553
            1995-2003           AA988423.1         19-27               c
            2004-7270           AC012100.7         46526-51792         c
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.2"
     gene            1..7270
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /note="signal peptide peptidase like 2A"
                     /db_xref="GeneID:84888"
                     /db_xref="HGNC:HGNC:30227"
                     /db_xref="MIM:608238"
     exon            1..239
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..1736
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /EC_number="3.4.23.-"
                     /note="intramembrane protease 3; presenilin-like protein
                     2; intramembrane cleaving protease; IMP-3; SPP-like 2A"
                     /codon_start=1
                     /product="signal peptide peptidase-like 2A precursor"
                     /protein_id="NP_116191.2"
                     /db_xref="CCDS:CCDS10138.1"
                     /db_xref="GeneID:84888"
                     /db_xref="HGNC:HGNC:30227"
                     /db_xref="MIM:608238"
                     /translation="MGPQRRLSPAGAALLWGFLLQLTAAQEAILHASGNGTTKDYCML
                     YNPYWTALPSTLENATSISLMNLTSTPLCNLSDIPPVGIKSKAVVVPWGSCHFLEKAR
                     IAQKGGAEAMLVVNNSVLFPPSGNRSEFPDVKILIAFISYKDFRDMNQTLGDNITVKM
                     YSPSWPNFDYTMVVIFVIAVFTVALGGYWSGLVELENLKAVTTEDREMRKKKEEYLTF
                     SPLTVVIFVVICCVMMVLLYFFYKWLVYVMIAIFCIASAMSLYNCLAALIHKIPYGQC
                     TIACRGKNMEVRLIFLSGLCIAVAVVWAVFRNEDRWAWILQDILGIAFCLNLIKTLKL
                     PNFKSCVILLGLLLLYDVFFVFITPFITKNGESIMVELAAGPFGNNEKLPVVIRVPKL
                     IYFSVMSVCLMPVSILGFGDIIVPGLLIAYCRRFDVQTGSSYIYYVSSTVAYAIGMIL
                     TFVVLVLMKKGQPALLYLVPCTLITASVVAWRRKEMKKFWKGNSYQMMDHLDCATNEE
                     NPVISGEQIVQQ"
     exon            240..350
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            351..533
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            534..623
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            624..757
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            758..906
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1003
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1105
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1187
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1262
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1319
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1422
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1500
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1661
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..7270
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1938..1943
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /note="hexamer: AATAAA"
     polyA_site      1967
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /note="major polyA site"
     regulatory      4036..4041
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /note="hexamer: AATAAA"
     polyA_site      4054
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
     regulatory      7247..7252
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /note="hexamer: AATAAA"
     polyA_site      7270
                     /gene="SPPL2A"
                     /gene_synonym="IMD86; IMP3; PSL2"
                     /note="major polyA site"
ORIGIN      
        1 agtcgcgctg ctgtggcggc cgctgtagca gcggcggtcc agtcgtagcc cggccgcccg
       61 cgcctgtccg gtccggtccg gccacggagg cagcgcagcg gcgggactcc gagcctaccc
      121 cgccgagtga gctgcgccgc accgtgccgt cccacccggc acccaccagt ccgatggggc
      181 cgcagcggcg gctgtcccct gccggggccg ccctactctg gggcttcctg ctccagctga
      241 cagccgctca ggaagcaatc ttgcatgcgt ctggaaatgg cacaaccaag gactactgca
      301 tgctttataa cccttattgg acagctcttc caagtaccct agaaaatgca acttccatta
      361 gtttgatgaa tctgacttcc acaccactat gcaacctttc tgatattcct cctgttggca
      421 taaagagcaa agcagttgtg gttccatggg gaagctgcca ttttcttgaa aaagccagaa
      481 ttgcacagaa aggaggtgct gaagcaatgt tagttgtcaa taacagtgtc ctatttcctc
      541 cctcaggtaa cagatctgaa tttcctgatg tgaaaatact gattgcattt ataagctaca
      601 aagactttag agatatgaac cagactctag gagataacat tactgtgaaa atgtattctc
      661 catcgtggcc taactttgat tatactatgg tggttatttt tgtaattgcg gtgttcactg
      721 tggcattagg tggatactgg agtggactag ttgaattgga aaacttgaaa gcagtgacaa
      781 ctgaagatag agaaatgagg aaaaagaagg aagaatattt aacttttagt cctcttacag
      841 ttgtaatatt tgtggtcatc tgctgtgtta tgatggtctt actttatttc ttctacaaat
      901 ggttggttta tgttatgata gcaattttct gcatagcatc agcaatgagt ctgtacaact
      961 gtcttgctgc actaattcat aagataccat atggacaatg cacgattgca tgtcgtggca
     1021 aaaacatgga agtgagactt atttttctct ctggactgtg catagcagta gctgttgttt
     1081 gggctgtgtt tcgaaatgaa gacaggtggg cttggatttt acaggatatc ttggggattg
     1141 ctttctgtct gaatttaatt aaaacactga agttgcccaa cttcaagtca tgtgtgatac
     1201 ttctaggcct tctcctcctc tatgatgtat tttttgtttt cataacacca ttcatcacaa
     1261 agaatggtga gagtatcatg gttgaactcg cagctggacc ttttggaaat aatgaaaagt
     1321 tgccagtagt catcagagta ccaaaactga tctatttctc agtaatgagt gtgtgcctca
     1381 tgcctgtttc aatattgggt tttggagaca ttattgtacc aggcctgttg attgcatact
     1441 gtagaagatt tgatgttcag actggttctt cttacatata ctatgtttcg tctacagttg
     1501 cctatgctat tggcatgata cttacatttg ttgttctggt gctgatgaaa aaggggcaac
     1561 ctgctctcct ctatttagta ccttgcacac ttattactgc ctcagttgtt gcctggagac
     1621 gtaaggaaat gaaaaagttc tggaaaggta acagctatca gatgatggac catttggatt
     1681 gtgcaacaaa tgaagaaaac cctgtgatat ctggtgaaca gattgtccag caataatatt
     1741 atgtggaact gctataatgt gtcattgatt ttctacaaat agacttcgac tttttaaatt
     1801 gacttttgaa ttgacaatct gaaagagtct tcaatgatat gcttgcaaaa atatattttt
     1861 atgagctggt actgacagtt acatcataaa taactaaaac gctttgcttt taatgttaaa
     1921 gttgtgcctt cacattaaat aaaacatatg gtctgtgtag tttccgagat gtactatata
     1981 cagtatattt ttctaaaaaa aaatgccttg acccacccct atactttttt ttaacgaaat
     2041 ctgtttcttt cagctctgat ggtagataca agtagttact atttctgaat gggttttact
     2101 gtaaagaatt atgcaaattg gccaggcgcg gtggctcacg cctgtaatcc caacactttg
     2161 ggaggccaag gcgggcggat cacgaggtca ggagatcgag accatcctgg ctaacacggt
     2221 gaaacccctc tctactaaaa ataccaaaaa ttagctgggc gtggtggagg gtgcctgtag
     2281 tcccagctac tgggaaggct gagacaggag aatggcgtga acccgggaag tggagcttgc
     2341 agtgagccaa gatcacgcca ctgcactcca gcctgggcga cagagtgaga ctccatccca
     2401 aaaaaaaaga tttatgcaaa ttatatctgc tctgtagaaa taactgttta acaccatgca
     2461 agaaggcggt tcaacggtga aaatttggac cttatatcac tgcagaggtt agaaatacat
     2521 aattttggaa atataagtgc atatttttgt agtatttttt taaacccaat cctttcataa
     2581 acttgagaac tgaaattact tcagactgca cgtgtattat attaagaaca tttttccaga
     2641 tatagagaaa cataatatat agaactagtc actaaaagga gacagacact gaaatctagt
     2701 tttcattggt ttgacagcct ttttttttta aatgtagctt aaatttcagt actaacattc
     2761 agtttcactt tggtatttct gatctttgca atttggttct gttaatgtta aggtttgatt
     2821 ttttaaaaaa attatttttc ttaagaaatt atgtattaat acatatggct catcgttgaa
     2881 tacagcattc ctaacattat cccacattcc cccactccca cccgttatct ttttttcttc
     2941 ctgatgccca ccccttttcc cataggtaat gattcttaat cttggtggag gatgtacaaa
     3001 gtgcggttca gagaaaagaa ttggcaacaa cagcaagaga cttggtgtta gtctcagttt
     3061 aattacaata caaggaagag ttgagtaatt tgccgcatct caagaccttg tatctcagtt
     3121 ttcttttttt tttttttttt ttttttgaga cggagtctcg ctctgtcgcc caggctggag
     3181 tgcagtggcg ggatctcggc tcactgcaag ctccgcctcc cgggttcacg ccattctcct
     3241 gcctcagcct cccaagtagc taggactaca ggcgcccgcc actacgcccg gctatttttt
     3301 gtagttttag tagagacggg gtttcaccgt tttagccggg atggtctcga tctcctgacc
     3361 tcgtgatccg cccgcctcgg cctcccaaag tgctgggatt acaggcgtga gccaccgcgc
     3421 ccggccatat ctcagttttc taatctgtaa aatgagtagg ttagaccagc taatcactct
     3481 ggtaccttcc aacttgaaaa aagttttttc cccattcttt ggtaaatttt agatgtggcc
     3541 tgaacattct cctggaagtc agaggtcaaa ttatctttcc attttctttt attttagtaa
     3601 gaatttcgca aatgacaagt gataacaaag gcagcaggat ttttcatttc tctgtaaata
     3661 atgtataaaa tatgtgaaca gaactatatc tttcatctaa ggccagttca atttattaaa
     3721 aataaaacta atgcctttta aaattgatca gacctttgat acttgaacta aattttgcat
     3781 tagagacttt ttaaaaattg ttcgcttttg ctgaataaat tgcaaataga gaagcttcat
     3841 tgacagtcag tccctctctg cttaatgcag ttatgggtcc tttcgtttat ggcaaatgac
     3901 ttacctgaca agagttaatt ttgcctccat tgtatcaagt ttgaatattt tccaagaaat
     3961 ctgatttatt tggcagactc tgagagagag aacagattaa gtgccgtttt aaaactctgt
     4021 attttcattt ataataataa aatatttgcc aaaatacagc tgttttgtaa aattatctgc
     4081 accctcatcc aagctttata tttaatagcc ttaatcacat tttatgaaga atgttaaaga
     4141 aaattttttt tttactacat cgaaatgaga taattaattt cttttttttt tttttggaaa
     4201 tggagtctcc ctctgtcacc caggctggag tgcagtagca agatcttggc tcactacaac
     4261 ctctgcctcc tggttcaagc aattttcctg cctcagcctc ccaagtagac gggattacag
     4321 gtgcgtgcca ccacacctgg ctaatttttg tatttttagt agagacgggg tttcaccttg
     4381 ttggtcaggc tggtcttgag ctcctgacct catgatctgc ccgcctcaga ctccccaagt
     4441 gctgggattg caggcatgag ccaccgcgcc tggcaaaata agataatttc tcaaaataat
     4501 ttggtttcat ataactggag tagtaattaa tattttaact tcatcaacta gatgtatctc
     4561 ctcagtagca tagtaactgg tacatccaga aatctgaaat ccgagttact tgatacaaat
     4621 tctactttat ctaaccatta gttattaagg aggtaaaatc ttgttctaat ttgctattaa
     4681 aatcttattg gttaaataga aataagaaaa agtataatta agtttcttca tcctcaatgt
     4741 caactttata ttgtaattaa atgtgtagca accaagctga caatagtgat taagaaatta
     4801 aagtacagaa atattttatt tggggactca aaatgaacaa aagatagata agattaaaat
     4861 ttcacttaaa aagctgccat ctcttcctct cttgccctcc caccaccaat aactttaagg
     4921 ggaagagaag ctggtttttt ccctaaacat gtctttaaaa taatgataca ctgtatttat
     4981 taattcttga tgcaaaaaaa aaaaagttag aaaatccctt tgaacatttt tgtggcttat
     5041 caacagaaat cactatctca aattgtaatg tagattactt ttgtctcagt ttagtttagc
     5101 aaaaataaat gtgaggcttg tggggaaaga ccttgatatt ctaatacatt atattttctt
     5161 ctagcttgta gatttttttt tttttttttt tttgagacag agtttcgctc tttttgccca
     5221 ggctggagtg caatggcatg atatcagctc actgcaatct ccgactcccg ggttcaagca
     5281 attctcctga ctcagcctcc tgagtagctg ggattatagg catgtgccac cacacccggc
     5341 taatatagta tttttagtag agacggggtt tctccatgtt gatcaggctg gtctcgaact
     5401 cccaacctga ggtgatccgc ccgcctcagc ctcctaaagt gctgggatta caggcatgag
     5461 ccaccgtgcc tggcacttgt agattttttt ttagattagc aattccttag tagaatctgc
     5521 aatttatttc tcttttaaag agaattttaa cccatctgct attggcattc ttatatttta
     5581 cttgtccata gagattttag tgaattgata catttataat aaatacaaat gagctttcca
     5641 gttttctttg ctctttcaat ttatgcttta gtataagcaa aaattactat ttttacattt
     5701 ttaaaaattg tgggtacatg taggtgtata tatttatgga gtgcatgaga tgttttgata
     5761 caggtatgca atgtgaaata agcggatcat ggagaatggg gtatccattc cctcaagcat
     5821 ttatcctttg agttatagac atattttaca tttgaataaa aaatatttga actaaggccg
     5881 ggtgcggtgg ctcacacctg taatcccagc actttgggag gccaaggcgg gtggatcatg
     5941 aggtcaggag tttgagacca gcctgaccaa catggtgaaa ccccgtctct actaaaaata
     6001 caaaaattag ctgggtgtgg tggggctcgc ctgtaacccc agctactcag gagactgagg
     6061 caggagaatc gcttgaacct gggaggtgga ggttgcagtg agtcgagatc acaccactgc
     6121 actccagcct gggcgacaga gcgagactcc atctcaaaaa aaaaaaaaaa aaaaaaaaat
     6181 atatatatgt atatatatat atatatatat gtatatatga actaatttat ttaggcttaa
     6241 tctctaaaat ttcagcctga tcttaaccaa tgaaatttca aaaacaaaaa ctgtacttca
     6301 gtctacaatt gaatggaatt cagtgtagac cagtacaatg gaatgtactg gctgagatgg
     6361 gagggtccct tgagcccagg agtttgaggc tgtagtgagc tgtgattgtg tctgtgaata
     6421 gtgactgcac tccagcctag gtgacatagc aagatgccat ctcaaagaaa tatagatata
     6481 taacataaaa taaataactt tatacatttc ctggaggaaa agtactctgt aaattcaaga
     6541 gatctgaatc aaattactca accttttgga atttgatcct ctactgtaat actcatatgc
     6601 tagattactg aattttcaga gtgaaaaaaa attgacaggg taatatatag gccatattat
     6661 agtgaagatc ataagtataa atgtcctcgt ttaatctttc catattgagg acagcctctg
     6721 catataaaaa tgttcattgg gagaaaaatt atttttaaaa ctaaattttt atagtagtcc
     6781 tccattactg gcaattaagg agcaacccac aaaatgtcat tatgtggttc tttgattagg
     6841 cataaaaagt attgcaaaca gcttttctat tcctttggaa tatgaatctt tttaatgtct
     6901 aaagttaacc tgcaacattt tctagtagga caagtcaatg taaattaggt aatttttgcc
     6961 tgttttatac cttctgttgc attttgttca aatgaaaaca ttttttaaaa catgattgaa
     7021 tttttacttc tcagataatt ggtttgagtg tggaatttct tttttaacac tttgatatgt
     7081 aagttagtgt aaattaatac ttttataaca ctactaggct ttatattttt atggaatgtt
     7141 ttaaaatttg aaatttttga agtgttaatt tagcagtact ttatcattat gtgccaatac
     7201 tgacaagaaa aacaattttt tatgtattta atatactatg gtattcaata aattctattt
     7261 taaaataaaa
//
