LOCUS       NM_001040058            1561 bp    mRNA    linear   PRI 06-MAR-2025
DEFINITION  Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant
            1, mRNA.
ACCESSION   NM_001040058
VERSION     NM_001040058.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Mortazavi,S.E., Lugaajju,A., Danielsson,L., Wu,B., Norrgren,H. and
            Persson,K.E.M.
  TITLE     Dynamics of osteopontin levels and correlation with parasitemia in
            acute malaria in Uganda and Sweden
  JOURNAL   BMC Infect Dis 24 (1), 1164 (2024)
   PUBMED   39407132
  REMARK    GeneRIF: Dynamics of osteopontin levels and correlation with
            parasitemia in acute malaria in Uganda and Sweden.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Zhang,Z., Chen,X., Li,Y., Zhang,F., Quan,Z., Wang,Z., Yang,Y.,
            Si,W., Xiong,Y., Ju,J., Bian,Y. and Sun,S.
  TITLE     The resistance to anoikis, mediated by Spp1, and the evasion of
            immune surveillance facilitate the invasion and metastasis of
            hepatocellular carcinoma
  JOURNAL   Apoptosis 29 (9-10), 1564-1583 (2024)
   PUBMED   39066845
  REMARK    GeneRIF: The resistance to anoikis, mediated by Spp1, and the
            evasion of immune surveillance facilitate the invasion and
            metastasis of hepatocellular carcinoma.
REFERENCE   3  (bases 1 to 1561)
  AUTHORS   Wang,Y., Zhang,W., Yang,Y., Qin,J., Wang,R., Wang,S., Fu,W.,
            Niu,Q., Wang,Y., Li,C., Li,H., Zhou,Y. and Liu,M.
  TITLE     Osteopontin deficiency promotes cartilaginous endplate degeneration
            by enhancing the NF-kappaB signaling to recruit macrophages and
            activate the NLRP3 inflammasome
  JOURNAL   Bone Res 12 (1), 53 (2024)
   PUBMED   39242551
  REMARK    GeneRIF: Osteopontin deficiency promotes cartilaginous endplate
            degeneration by enhancing the NF-kappaB signaling to recruit
            macrophages and activate the NLRP3 inflammasome.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1561)
  AUTHORS   Jansson,K.P., Kuluva,J., Zhang,S., Swanson,T., Zhang,Y.,
            Zimmerman,K.A., Fields,T.A., Wallace,D.P., Rowe,P.S. and
            Stubbs,J.R.
  TITLE     Osteopontin deletion attenuates cyst growth but exacerbates
            fibrosis in mice with cystic kidney disease
  JOURNAL   Physiol Rep 12 (17), e70038 (2024)
   PUBMED   39238069
  REMARK    GeneRIF: Osteopontin deletion attenuates cyst growth but
            exacerbates fibrosis in mice with cystic kidney disease.
REFERENCE   5  (bases 1 to 1561)
  AUTHORS   Kupryte,M., Lesauskaite,V., Sirataviciute,V., Utkiene,L.,
            Jusiene,L. and Pangonyte,D.
  TITLE     Expression of Osteopontin and Gremlin 1 Proteins in Cardiomyocytes
            in Ischemic Heart Failure
  JOURNAL   Int J Mol Sci 25 (15), 8240 (2024)
   PUBMED   39125809
  REMARK    GeneRIF: Expression of Osteopontin and Gremlin 1 Proteins in
            Cardiomyocytes in Ischemic Heart Failure.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1561)
  AUTHORS   Kohri,K., Suzuki,Y., Yoshida,K., Yamamoto,K., Amasaki,N.,
            Yamate,T., Umekawa,T., Iguchi,M., Sinohara,H. and Kurita,T.
  TITLE     Molecular cloning and sequencing of cDNA encoding urinary stone
            protein, which is identical to osteopontin
  JOURNAL   Biochem Biophys Res Commun 184 (2), 859-864 (1992)
   PUBMED   1575754
REFERENCE   7  (bases 1 to 1561)
  AUTHORS   Shiraga,H., Min,W., VanDusen,W.J., Clayman,M.D., Miner,D.,
            Terrell,C.H., Sherbotie,J.R., Foreman,J.W., Przysiecki,C.,
            Neilson,E.G. et al.
  TITLE     Inhibition of calcium oxalate crystal growth in vitro by uropontin:
            another member of the aspartic acid-rich protein superfamily
  JOURNAL   Proc Natl Acad Sci U S A 89 (1), 426-430 (1992)
   PUBMED   1729712
REFERENCE   8  (bases 1 to 1561)
  AUTHORS   Young,M.F., Kerr,J.M., Termine,J.D., Wewer,U.M., Wang,M.G.,
            McBride,O.W. and Fisher,L.W.
  TITLE     cDNA cloning, mRNA distribution and heterogeneity, chromosomal
            location, and RFLP analysis of human osteopontin (OPN)
  JOURNAL   Genomics 7 (4), 491-502 (1990)
   PUBMED   1974876
REFERENCE   9  (bases 1 to 1561)
  AUTHORS   Fisher,L.W., McBride,O.W., Termine,J.D. and Young,M.F.
  TITLE     Human bone sialoprotein. Deduced protein sequence and chromosomal
            localization
  JOURNAL   J Biol Chem 265 (4), 2347-2351 (1990)
   PUBMED   2404984
REFERENCE   10 (bases 1 to 1561)
  AUTHORS   Kiefer,M.C., Bauer,D.M. and Barr,P.J.
  TITLE     The cDNA and derived amino acid sequence for human osteopontin
  JOURNAL   Nucleic Acids Res 17 (8), 3306 (1989)
   PUBMED   2726470
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB117856.1, BC017387.1 and
            AA665210.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001040058.1.
            
            Summary: The protein encoded by this gene is involved in the
            attachment of osteoclasts to the mineralized bone matrix. The
            encoded protein is secreted and binds hydroxyapatite with high
            affinity. The osteoclast vitronectin receptor is found in the cell
            membrane and may be involved in the binding to this protein. This
            protein is also a cytokine that upregulates expression of
            interferon-gamma and interleukin-12. Several transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (1) differs in the 5' UTR and
            coding sequence compared to variant 5. The resulting isoform
            (OPN-a, as described in PubMed: 7837791) has a shorter and distinct
            N-terminus compared to isoform e.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1143592.1,
                                           SRR3476690.158658.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000395080.8/ ENSP00000378517.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 CB117856.1         65-65
            2-1468              BC017387.1         6-1472
            1469-1561           AA665210.1         135-227
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q22.1"
     gene            1..1561
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /note="secreted phosphoprotein 1"
                     /db_xref="GeneID:6696"
                     /db_xref="HGNC:HGNC:11255"
                     /db_xref="MIM:166490"
     exon            1..87
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /inference="alignment:Splign:2.1.0"
     exon            88..155
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..1046
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /note="isoform OPN-a precursor is encoded by transcript
                     variant 1; secreted phosphoprotein 1 (osteopontin, bone
                     sialoprotein I, early T-lymphocyte activation 1);
                     SPP1/CALPHA1 fusion; osteopontin/immunoglobulin alpha 1
                     heavy chain constant region fusion protein; uropontin;
                     lnc-PKD2-2-3; nephropontin; urinary stone protein"
                     /codon_start=1
                     /product="osteopontin isoform OPN-a precursor"
                     /protein_id="NP_001035147.1"
                     /db_xref="CCDS:CCDS43250.1"
                     /db_xref="GeneID:6696"
                     /db_xref="HGNC:HGNC:11255"
                     /db_xref="MIM:166490"
                     /translation="MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWL
                     NPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDS
                     IDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRG
                     DSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWD
                     SRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSH
                     EFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN"
     exon            156..194
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /inference="alignment:Splign:2.1.0"
     exon            195..275
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /inference="alignment:Splign:2.1.0"
     exon            276..317
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /inference="alignment:Splign:2.1.0"
     exon            318..641
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /inference="alignment:Splign:2.1.0"
     exon            642..1561
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1454..1459
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /note="hexamer: AATAAA"
     polyA_site      1484
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /note="major polyA site"
     regulatory      1534..1539
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
                     /note="hexamer: AATAAA"
     polyA_site      1561
                     /gene="SPP1"
                     /gene_synonym="BNSP; BSPI; ETA-1; OPN"
ORIGIN      
        1 agcagcagga ggaggcagag cacagcatcg tcgggaccag actcgtctca ggccagttgc
       61 agccttctca gccaaacgcc gaccaaggaa aactcactac catgagaatt gcagtgattt
      121 gcttttgcct cctaggcatc acctgtgcca taccagttaa acaggctgat tctggaagtt
      181 ctgaggaaaa gcagctttac aacaaatacc cagatgctgt ggccacatgg ctaaaccctg
      241 acccatctca gaagcagaat ctcctagccc cacagaatgc tgtgtcctct gaagaaacca
      301 atgactttaa acaagagacc cttccaagta agtccaacga aagccatgac cacatggatg
      361 atatggatga tgaagatgat gatgaccatg tggacagcca ggactccatt gactcgaacg
      421 actctgatga tgtagatgac actgatgatt ctcaccagtc tgatgagtct caccattctg
      481 atgaatctga tgaactggtc actgattttc ccacggacct gccagcaacc gaagttttca
      541 ctccagttgt ccccacagta gacacatatg atggccgagg tgatagtgtg gtttatggac
      601 tgaggtcaaa atctaagaag tttcgcagac ctgacatcca gtaccctgat gctacagacg
      661 aggacatcac ctcacacatg gaaagcgagg agttgaatgg tgcatacaag gccatccccg
      721 ttgcccagga cctgaacgcg ccttctgatt gggacagccg tgggaaggac agttatgaaa
      781 cgagtcagct ggatgaccag agtgctgaaa cccacagcca caagcagtcc agattatata
      841 agcggaaagc caatgatgag agcaatgagc attccgatgt gattgatagt caggaacttt
      901 ccaaagtcag ccgtgaattc cacagccatg aatttcacag ccatgaagat atgctggttg
      961 tagaccccaa aagtaaggaa gaagataaac acctgaaatt tcgtatttct catgaattag
     1021 atagtgcatc ttctgaggtc aattaaaagg agaaaaaata caatttctca ctttgcattt
     1081 agtcaaaaga aaaaatgctt tatagcaaaa tgaaagagaa catgaaatgc ttctttctca
     1141 gtttattggt tgaatgtgta tctatttgag tctggaaata actaatgtgt ttgataatta
     1201 gtttagtttg tggcttcatg gaaactccct gtaaactaaa agcttcaggg ttatgtctat
     1261 gttcattcta tagaagaaat gcaaactatc actgtatttt aatatttgtt attctctcat
     1321 gaatagaaat ttatgtagaa gcaaacaaaa tacttttacc cacttaaaaa gagaatataa
     1381 cattttatgt cactataatc ttttgttttt taagttagtg tatattttgt tgtgattatc
     1441 tttttgtggt gtgaataaat cttttatctt gaatgtaata agaatttggt ggtgtcaatt
     1501 gcttatttgt tttcccacgg ttgtccagca attaataaaa cataaccttt tttactgcct
     1561 a
//
