LOCUS       NM_016390               4259 bp    mRNA    linear   PRI 22-JAN-2025
DEFINITION  Homo sapiens SPOUT domain containing methyltransferase 1 (SPOUT1),
            mRNA.
ACCESSION   NM_016390
VERSION     NM_016390.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Chen,J., Bai,Y., Huang,Y., Cui,M., Wang,Y., Gu,Z., Wu,X., Li,Y. and
            Rong,Y.S.
  TITLE     The Ptch/SPOUT1 methyltransferase deposits an m3U modification on
            28S rRNA for normal ribosomal function in flies and humans
  JOURNAL   Sci Adv 10 (50), eadr1743 (2024)
   PUBMED   39671501
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Treiber,T., Treiber,N., Plessmann,U., Harlander,S., Daiss,J.L.,
            Eichner,N., Lehmann,G., Schall,K., Urlaub,H. and Meister,G.
  TITLE     A Compendium of RNA-Binding Proteins that Regulate MicroRNA
            Biogenesis
  JOURNAL   Mol Cell 66 (2), 270-284 (2017)
   PUBMED   28431233
REFERENCE   3  (bases 1 to 4259)
  AUTHORS   Ohta,S., Wood,L., Toramoto,I., Yagyu,K., Fukagawa,T. and
            Earnshaw,W.C.
  TITLE     CENP-32 is required to maintain centrosomal dominance in bipolar
            spindle assembly
  JOURNAL   Mol Biol Cell 26 (7), 1225-1237 (2015)
   PUBMED   25657325
  REMARK    GeneRIF: CENP-32 appears to be required for centrosomes to
            integrate into a fully functional spindle that not only nucleates
            astral microtubules, but also is able to nucleate and bind to
            kinetochore and central spindle microtubules.
REFERENCE   4  (bases 1 to 4259)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   5  (bases 1 to 4259)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (bases 1 to 4259)
  AUTHORS   Zhang,F., Baumer,N., Rode,M., Ji,P., Zhang,T., Berdel,W.E. and
            Muller-Tidow,C.
  TITLE     The inhibitor of growth protein 5 (ING5) depends on INCA1 as a
            co-factor for its antiproliferative effects
  JOURNAL   PLoS One 6 (7), e21505 (2011)
   PUBMED   21750715
REFERENCE   7  (bases 1 to 4259)
  AUTHORS   Ohta,S., Bukowski-Wills,J.C., Sanchez-Pulido,L., Alves Fde,L.,
            Wood,L., Chen,Z.A., Platani,M., Fischer,L., Hudson,D.F.,
            Ponting,C.P., Fukagawa,T., Earnshaw,W.C. and Rappsilber,J.
  TITLE     The protein composition of mitotic chromosomes determined using
            multiclassifier combinatorial proteomics
  JOURNAL   Cell 142 (5), 810-821 (2010)
   PUBMED   20813266
REFERENCE   8  (bases 1 to 4259)
  AUTHORS   Andersen,J.S., Lyon,C.E., Fox,A.H., Leung,A.K., Lam,Y.W., Steen,H.,
            Mann,M. and Lamond,A.I.
  TITLE     Directed proteomic analysis of the human nucleolus
  JOURNAL   Curr Biol 12 (1), 1-11 (2002)
   PUBMED   11790298
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL110193.1, BC046133.1, BC063644.1, AL441992.17 and BM997763.1.
            
            On Nov 23, 2018 this sequence version replaced NM_016390.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL110193.1, SRR11853564.27977.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361256.10/ ENSP00000354812.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AL110193.1         28-35
            9-1119              BC046133.1         1-1111
            1120-1844           BC063644.1         1115-1839
            1845-2487           AL110193.1         1872-2514
            2488-2488           AL441992.17        122008-122008       c
            2489-3840           AL110193.1         2516-3867
            3841-4259           BM997763.1         17-435              c
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.11"
     gene            1..4259
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /note="SPOUT domain containing methyltransferase 1"
                     /db_xref="GeneID:51490"
                     /db_xref="HGNC:HGNC:26933"
                     /db_xref="MIM:617614"
     exon            1..50
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1145
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /EC_number="2.1.1.-"
                     /note="centromere protein 32; kinetochore-associated
                     protein"
                     /codon_start=1
                     /product="putative methyltransferase C9orf114"
                     /protein_id="NP_057474.2"
                     /db_xref="CCDS:CCDS6913.1"
                     /db_xref="GeneID:51490"
                     /db_xref="HGNC:HGNC:26933"
                     /db_xref="MIM:617614"
                     /translation="MAERGRKRPCGPGEHGQRIEWRKWKQQKKEEKKKWKDLKLMKKL
                     ERQRAQEEQAKRLEEEEAAAEKEDRGRPYTLSVALPGSILDNAQSPELRTYLAGQIAR
                     ACAIFCVDEIVVFDEEGQDAKTVEGEFTGVGKKGQACVQLARILQYLECPQYLRKAFF
                     PKHQDLQFAGLLNPLDSPHHMRQDEESEFREGIVVDRPTRPGHGSFVNCGMKKEVKID
                     KNLEPGLRVTVRLNQQQHPDCKTYHGKVVSSQDPRTKAGLYWGYTVRLASCLSAVFAE
                     APFQDGYDLTIGTSERGSDVASAQLPNFRHALVVFGGLQGLEAGADADPNLEVAEPSV
                     LFDLYVNTCPGQGSRTIRTEEAILISLAALQPGLIQAGARHT"
     exon            51..96
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     exon            97..222
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     exon            223..382
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     exon            383..472
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     exon            473..522
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     exon            523..653
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     exon            654..726
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     exon            727..825
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     exon            826..928
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1076
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..4259
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1821..1826
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /note="hexamer: AATAAA"
     polyA_site      1840
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /note="major polyA site"
     regulatory      4238..4243
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
                     /note="hexamer: AATAAA"
     polyA_site      4259
                     /gene="SPOUT1"
                     /gene_synonym="C9orf114; CENP-32; CENP32; HSPC109"
ORIGIN      
        1 ggtgtgtgcg gaacatggcg gagcgcggca ggaagcggcc gtgcggcccg ggtgaacacg
       61 gccaaaggat tgagtggcga aaatggaagc aacagaagaa agaggagaaa aaaaaatgga
      121 aggatctcaa gctgatgaaa aaactggagc ggcagcgggc acaggaggaa caggcaaagc
      181 gcctggaaga ggaggaggca gcggcagaga aggaggaccg cgggcggccc tacacactga
      241 gcgtagccct gccgggctcc atcctggaca atgctcagtc gccggagctt cgcacctact
      301 tggccggtca gattgccaga gcctgtgcca tcttctgtgt ggatgagatc gtggtgtttg
      361 atgaggaggg ccaggatgcc aagactgtgg agggggaatt cacaggagtt gggaagaagg
      421 ggcaggcgtg cgtacagctg gcccggatcc tgcagtacct ggagtgtcca cagtacctga
      481 ggaaggcgtt cttccccaag caccaggatc tacagtttgc agggctcctg aaccccctgg
      541 acagccccca ccacatgcgt caggatgagg aatccgagtt ccgagagggc atcgtggtgg
      601 atcggcccac ccggccaggc cacggctcct ttgtcaactg tggcatgaaa aaggaggtga
      661 agattgacaa gaacctggag cccgggcttc gggtgactgt gcgactgaac cagcagcagc
      721 acccagactg caagacctac catggcaaag tggtatcatc gcaggaccct cgcaccaaag
      781 ctggtctcta ctggggctac accgtccgac tggcttcctg cctcagtgct gtgtttgctg
      841 aggccccctt ccaagatggg tatgacctga ccatcgggac gtcagagcgc ggctcagatg
      901 tggcctctgc ccagcttccc aacttcaggc atgctcttgt ggtgttcggg ggcctccagg
      961 gtctggaagc tggagcggat gctgacccca acctggaggt ggctgaaccc agtgtcctct
     1021 ttgacctgta cgtcaatacc tgtcctggcc agggtagccg taccatccgc acggaggaag
     1081 ccatcctcat ctccctggcc gccctgcagc ctggcctcat ccaggcgggt gcccggcaca
     1141 cctgaaagtt ctaaggggcc gaggacatca gtgaagcagc agtgaaacca ggggctctgc
     1201 aggtcacttg ggacggacgc caccagactt gtctccaaaa atcaccacct ttaatactcc
     1261 ccggcctgca cacacccaca gtctcactgg gctccaccct cacttactgc ccgccgtgat
     1321 ggccttgagg ctgcctgccc gcgccaggat gtttggcaca aagagcagcc ccgaagcccg
     1381 ctcaatgctc tcgatgggca ccaggaagcg ctccagtggg atggcctcat ccacaggtgc
     1441 gttgggcatc acgtaggtgc ggagctcaat ttgcccacct gctgcctcca ggatcagcac
     1501 cttgaagaag tgtgtgggca ctgccacgtg gttcttgccg atgacctggt actttacgta
     1561 ggatttccca tcagcctctg tcctgtgggc agacccaggc acacgtgggc agggggcctg
     1621 gatgaaccca aagccacctc ttccaggcag ccttccctga tctgtcctct aacttctgtc
     1681 aacctgcagg accacgccct cactgtgaga cttctgtagc ctgcctggct cctccttgag
     1741 gctggaactc tctccagggt ggttaactct ttccctctgt tttccttatg cccaacactg
     1801 ggtcaggcag agtgggcgac aataaacgct gctaggagaa tcgagccttc tgaatattca
     1861 cattcgtctg aggggagact taacgactgt ccatctctcc ccacagaggt gagcaacaag
     1921 tgggcaagga ccatgacagg tgatatccca gcaccagcac aggtgccagg acaccggacg
     1981 ggggctcccc ctcagcagag tctcctccct tccaccaggc cctaggaggc acctgctaat
     2041 gaaatttgat cagtgcccca accaccgcct tggccagcct atgccgagcc cttaccatgg
     2101 accaggccct ggggaagcat gtgacatcaa ccaagaggtc acaggttttg cagaggggga
     2161 cacaggtcat ttgcccacag caccaaggaa ctaggtgatg aggcctggac tccatcccga
     2221 gcctgtggat tccagagtga gctgctcagc ctggacctgt tgcctctcag ccccagccct
     2281 ctgcaggcca gaaaaaccac ggagcacacc cagaggtgct cagttgcttc cacctgagag
     2341 gcagggctgc acaggagcaa gagtggaggc cctgggtcag gcagatctag attcaaactc
     2401 ccgagaaggg tggagggaag tgctccaagc agagcagaga ctgaaggcgg gagagcctgg
     2461 tgcaccgcgg acctgcaggg ggaaggcatg agagctcggt gcactgggga cctgcatggg
     2521 gaaggcggga gagctcggtg cactggggac ctgcatgggg aaggcgtgag agctcggtgc
     2581 actggggacc tgcatgggga aggcgggaga gctcggtgca cctcggacct gcagagggaa
     2641 ggtgggagag cttggtgcac cgcagacctg cagggggaag gcgggagagc ttggtgcact
     2701 gcagacctgc ggggggaagg tgggagagct tggtgcactg cagacctgcg gggggttcca
     2761 tattggcaga acaagattgg gtgggcatgc caagaggtga cagcagggag aagcgggggc
     2821 agattcaggg cctgctgctg cccatgggca gaggagagat ggtcaggggt acccgacagg
     2881 tctttggctg tggaggtgga atgaggggac tcgaggcagg gaagctgaca ggcttgccaa
     2941 gaaaccagat gagtagggaa ctggtattga cccagagcct ggggcaagcc tggccctgtg
     3001 agtggcagta ggggcctcag gtgagtggga ggttctgccg cccccctggt ttccacctta
     3061 cctgggcagg aagagtggcc ctgtgcagac atagacgttt tggtagctgc gggtcaagct
     3121 gcggctatat ttctccaggt tgttccaggc attctggttg aggtggggca cctggtagga
     3181 gagatagggc ttagcaggac tgtggctggg acccttcctc ctctatcaga gggccgcccc
     3241 aggtcctgag gggatggaca ggctcagaga tatccagtga gacaagcggg gggaaactga
     3301 ggctcaagga atgaaagtca ctccctgaaa cccagggctg atagagtgct agccaccacc
     3361 ctctgtccct cccacagccc aggtgtcaaa gtcttttctc agctcccaag agtcgaatga
     3421 aggaagagcc tgtctccacc tttcagagag gactgaggcc tgtccccagc cccacccagg
     3481 gtctcctggg aagaccagcc cttccaacta ccaacccgtt ccttttccca gtctgagcca
     3541 caggaagagc ctagcgggga atgccatgaa tcgacctcca tcctgagctc tccaggcctg
     3601 ggacaatgga aagtggatag ggggctgtct tcccagaagg aagctgggtc agaggttggt
     3661 gccccatggg ctccacccag agccccatgg cagtctccat ccattggtgc caggacctgc
     3721 tgggaacagg ctgagagggt ggttggccca gtgcacagtg cctggcacta acaacctgat
     3781 cgtgggccag gtgtctgcct cgaaactgcc tgaaggcagg cagggtagga caatgtctgg
     3841 aggctcacgg ttttatagac taggggtccc caacccctgg gctacagacc ggtaccagaa
     3901 ccgggccaca cagcaggagg tgagcagcag gcgagcaagg gaagcttcat ctgtatttac
     3961 agccaccccc ccattgatcc cacagcagcc tgagctccgc ctcctgtcag atcagtggcg
     4021 gctggagatt gtcataggag ctggaaccct atggtgaact gcgcatgtga ggaggttgcg
     4081 cgcttctttt gaaaacctaa tgcctgatct gtcgttgttg cccatcacct ccagatagga
     4141 ctgtctagtt gcaggaaaac aagctcaggg ctcccacgga tcctacatta ctgtgagttg
     4201 tatatttcac tatatgttac aatgtaataa taatagaaat aaaatgtaca taaatgtaa
//
