LOCUS       NM_001001664            6056 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens speckle type BTB/POZ protein like (SPOPL), mRNA.
ACCESSION   NM_001001664 XM_376144
VERSION     NM_001001664.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Hu,T., Xuan,R., Han,E., Cai,L. and Xia,Z.
  TITLE     SPOPL induces tumorigenicity and stemness in glioma stem cells by
            activating Notch signaling
  JOURNAL   J Neurooncol 164 (1), 157-170 (2023)
   PUBMED   37523046
  REMARK    GeneRIF: SPOPL induces tumorigenicity and stemness in glioma stem
            cells by activating Notch signaling.
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 6056)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6056)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6056)
  AUTHORS   Gschweitl,M., Ulbricht,A., Barnes,C.A., Enchev,R.I.,
            Stoffel-Studer,I., Meyer-Schaller,N., Huotari,J., Yamauchi,Y.,
            Greber,U.F., Helenius,A. and Peter,M.
  TITLE     A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic
            trafficking by targeting EPS15 at endosomes
  JOURNAL   Elife 5, e13841 (2016)
   PUBMED   27008177
  REMARK    GeneRIF: Together, the data indicate that a CUL3-SPOPL E3 ubiquitin
            ligase complex regulates endocytic trafficking and formation of
            multivesicular bodies by ubiquitinating and degrading EPS15 at
            endosomes.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6056)
  AUTHORS   Clark,S.L., Souza,R.P., Adkins,D.E., Aberg,K., Bukszar,J.,
            McClay,J.L., Sullivan,P.F. and van den Oord,E.J.
  TITLE     Genome-wide association study of patient-rated and clinician-rated
            global impression of severity during antipsychotic treatment
  JOURNAL   Pharmacogenet Genomics 23 (2), 69-77 (2013)
   PUBMED   23241943
REFERENCE   7  (bases 1 to 6056)
  AUTHORS   Errington,W.J., Khan,M.Q., Bueler,S.A., Rubinstein,J.L.,
            Chakrabartty,A. and Prive,G.G.
  TITLE     Adaptor protein self-assembly drives the control of a cullin-RING
            ubiquitin ligase
  JOURNAL   Structure 20 (7), 1141-1153 (2012)
   PUBMED   22632832
REFERENCE   8  (bases 1 to 6056)
  AUTHORS   Zhang,Q., Zhang,L., Wang,B., Ou,C.Y., Chien,C.T. and Jiang,J.
  TITLE     A hedgehog-induced BTB protein modulates hedgehog signaling by
            degrading Ci/Gli transcription factor
  JOURNAL   Dev Cell 10 (6), 719-729 (2006)
   PUBMED   16740475
REFERENCE   9  (bases 1 to 6056)
  AUTHORS   Kent,D., Bush,E.W. and Hooper,J.E.
  TITLE     Roadkill attenuates Hedgehog responses through degradation of
            Cubitus interruptus
  JOURNAL   Development 133 (10), 2001-2010 (2006)
   PUBMED   16651542
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114763.6 and AC092620.2.
            
            On May 17, 2019 this sequence version replaced NM_001001664.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC071613.1, SRR11853558.14691.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000280098.9/ ENSP00000280098.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-350               AC114763.6         45529-45878
            351-488             AC092620.2         142233-142370       c
            489-610             AC092620.2         141923-142044       c
            611-762             AC092620.2         141473-141624       c
            763-890             AC092620.2         139846-139973       c
            891-1068            AC092620.2         133328-133505       c
            1069-1124           AC092620.2         133190-133245       c
            1125-1247           AC092620.2         131600-131722       c
            1248-1390           AC092620.2         127677-127819       c
            1391-1444           AC092620.2         127534-127587       c
            1445-6056           AC092620.2         118980-123591       c
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q22.1"
     gene            1..6056
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /note="speckle type BTB/POZ protein like"
                     /db_xref="GeneID:339745"
                     /db_xref="HGNC:HGNC:27934"
     exon            1..350
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     exon            351..488
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     CDS             411..1589
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /note="HIB homolog 2; roadkill homolog 2"
                     /codon_start=1
                     /product="speckle-type POZ protein-like"
                     /protein_id="NP_001001664.1"
                     /db_xref="CCDS:CCDS33298.1"
                     /db_xref="GeneID:339745"
                     /db_xref="HGNC:HGNC:27934"
                     /translation="MSREPTPPLPGDMSTGPIAESWCYTQVKVVKFSYMWTINNFSFC
                     REEMGEVLKSSTFSSGPSDKMKWCLRVNPKGLDDESKDYLSLYLLLVSCPKSEVRAKF
                     KFSLLNAKREETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPDDKLTLFCE
                     VSVVQDSVNISGHTNTNTLKVPECRLAEDLGNLWENTRFTDCSFFVRGQEFKAHKSVL
                     AARSPVFNAMFEHEMEESKKNRVEINDLDPEVFKEMMRFIYTGRAPNLDKMADNLLAA
                     ADKYALERLKVMCEEALCSNLSVENVADTLVLADLHSAEQLKAQAIDFINRCSVLRQL
                     GCKDGKNWNSNQATDIMETSGWKSMIQSHPHLVAEAFRALASAQCPQFGIPRKRLKQS
                     "
     exon            489..610
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     exon            611..762
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     exon            763..890
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1068
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1124
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1247
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1390
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1444
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..6056
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4803..4808
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /note="hexamer: AACAAA"
     polyA_site      4819
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /note="major polyA site"
     regulatory      5714..5719
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /note="hexamer: AATAAA"
     polyA_site      5744
                     /gene="SPOPL"
                     /gene_synonym="BTBD33"
                     /note="major polyA site"
ORIGIN      
        1 acgcgccctc gcgggctgga gccggggctg gagtcgtaac tcggaagccg gagcccagac
       61 gggcccgcgg cgggggggtg ggggctgcgc gggggcggga gtggcggagg tggccgcccc
      121 tggcggcggg tttgtttatc ccggggaaga agtttaggag cgggagatag ggaaggaggg
      181 cgggtcgggg aggagggatg cggttcggcg gaggcggccg ccacagggac ttgccgccat
      241 cacccctgct gccaccaccg cagcctcggg ctcccagggc ggacacggcc accgcctcag
      301 cgggagagga gtctccacca ggactgaccg ctgccgccca gcacgtccag gtaaggtact
      361 caactgtgtg gggtactaca taaatcctga aagactacaa taaagtggtg atgtctcggg
      421 aacccacccc acctctacct ggagatatgt ctactggtcc catagcagaa agctggtgtt
      481 acacacaggt taaagtagta aaattttcct atatgtggac cattaataac ttcagttttt
      541 gtcgagagga aatgggtgaa gtgttaaaaa gttcaacatt ttcatctggc ccaagtgaca
      601 aaatgaaatg gtgcctgagg gtaaacccaa agggattaga tgatgaaagt aaagactact
      661 tgtccttata tttgctttta gtcagctgcc ccaaaagtga agttcgagca aaattcaaat
      721 tttcccttct gaatgctaaa agggaagaaa caaaagcaat ggaaagccaa agagcatatc
      781 gatttgtgca agggaaggac tggggtttta aaaaattcat tagaagggac tttttgcttg
      841 atgaagctaa tggtctttta ccagatgaca agcttacatt attttgtgag gtgagtgtgg
      901 tccaagattc agtaaacata tcaggacata ctaatacaaa tactttgaag gtgcctgagt
      961 gtcgtctagc agaagattta ggtaatctct gggaaaacac aagatttaca gactgcagtt
     1021 ttttcgtgag aggacaagaa tttaaagctc ataaatctgt gcttgcagct cgatctccag
     1081 tttttaacgc catgtttgaa catgaaatgg aagaaagcaa aaagaatcga gtggaaataa
     1141 atgatttaga ccctgaagtt tttaaagaaa tgatgagatt catttacaca gggagagcac
     1201 caaaccttga caaaatggct gacaacttgt tggcagctgc agacaaatat gcactggaac
     1261 ggctgaaggt catgtgcgaa gaagctttgt gtagtaacct ctcagtagag aatgttgcag
     1321 atacccttgt ccttgcagat ttgcacagtg cagaacagtt gaaagcacaa gccatagact
     1381 ttattaatag gtgcagtgta cttcgacaac ttgggtgtaa agatgggaaa aactggaaca
     1441 gcaaccaagc aaccgacata atggaaacat cagggtggaa gtccatgatt cagtctcacc
     1501 ctcatttagt agcagaagcc tttcgagcac tagcatctgc acagtgtcca cagtttggca
     1561 ttccacgcaa acggctaaaa cagtcctgaa atcttccatg aacagttgaa aaatggaatt
     1621 gactttcact cctccaggtc cagaaggatt ctaatacaca aaccataagc aagagttgtt
     1681 tctgttattt tgtccacaga acagaagctg aaaaagcata ttgcttgcat ttcaggtgga
     1741 taatttatgg tttattcttc agctttaaat tagactgatt aattcacttc aaggccttaa
     1801 attatcttca atgacttgtc ttgttcatat aatactttaa ttttttttta ttgtgccttg
     1861 tcattttgac caaggctatg caggattgca ctagctccat aatgcagtaa tattgataac
     1921 tgaagatact aagtttcaaa aggatcttcc attattttgc aaaaagaaaa atgaatttta
     1981 tagggtttgt cctatgctat ctcaaagttt aagttctctt taaaagcact tgtattggag
     2041 attaccagta atatctccaa tctaagttct ataaatatgg gagaaccctc ttaccttcaa
     2101 ggtaagttat ggcaatacac tgcttcaatt ctaatttatt tttcatttca gggggcaaat
     2161 atgcaatgag ttggcctaga tttttagtga catttatgat gtttgtcttg tatgttaact
     2221 gtccaacaaa ctgtgggttt attctaagtt tacaattatt gagaactgat tgaggttaag
     2281 atttcatgaa gaaagcatgt attgtgtgga agtaattttt ttttaattta tactgaccta
     2341 catttatacg aagaattctg tacatgttaa aagtaaggat gaccaaatgc aaatttaatg
     2401 agtgggccaa ttaagaaaga tacatatgca cttttactgt gtaacttttg tatgctgaat
     2461 ggtacatata tttttttgct tttgagggaa ttaataaggt ataattaatt gtgtcttaac
     2521 ttttcaaaga aatttttaga tggaggcaac tgggatgttt gtgataatag atgagaaaga
     2581 catccttgat tgtagttaaa ttggtttgga ttacagatat tcattattga attgactcct
     2641 attttctcac actttggaaa agacacctga acaataaatg aatcctggat agtctactct
     2701 ccttcaaaca attaactgat acttgccttt gggagatgca tacaaaagga gagaaccctt
     2761 agactatgtc aggtcacact aggaacctcc cctcactccc cgtttttctt cattgtgttt
     2821 cagttactgt actaacattg tggatgatac tgaaattctg cataatgtga acaggataaa
     2881 ctatttataa actgcttttc atgggccagt tctttattat aaatgaattt tagctttaaa
     2941 tgcatataca cgttcagtgt ttgatagttg tgttcttaag ggaggtttgt tctggtgcat
     3001 tgtcctggag aagcagaagc agtcagtgtt ggggtaccac atctgactta ctgtggtggt
     3061 gaccagtgtg cttcttaagg gcaagattta tatttgacca caagggaaaa agtgtgtcac
     3121 tggaaaaaaa cgcatatacc tatgtttgca aacataacta ttcttcagat taaaatttgt
     3181 ataatttgtt tatttaatgt ttgaattcag gacaaaagaa aaaatgaagc ttgaggtgtt
     3241 ttcaaataga ccttattttt gctatctctt tgttaggtgt taactgaagc aattcgaata
     3301 tgtccagatt catttggatt catatttaca ttgaaaaaac tcaactaagc atttgttatc
     3361 cacaacaacc tagagtttaa aaagatcgta ttttcacctg tcttcattac tttctatgta
     3421 gtttcattct ttgtcgtggt aacttaaatt tgaaattgga agaatctctt gggatagaag
     3481 aaagattctt acctggtgat ggaacttcat acttcatcct gtgtaagagt attatgggaa
     3541 gtagaaattc acttccttaa atccatcgta agattgagcc tcaagtttgc aatatacaat
     3601 tttaaaaata cacatacata catacatatg tatacatttc cagttttaag attttgcgag
     3661 ggtcttataa gaaaacaaaa attccctcag gctatagaat tatgttgtca tatatcagaa
     3721 aagtactgat gtatccattt atatccaatg cgcaccacac cggcacattg tgatttaatt
     3781 caccgcttga atctatattt ctaaccacag tgacttcagt aaaaataccg tataatgaac
     3841 atttcagctt cttcttactt actcgagagt ttattgcaaa tcttaaggat tttattataa
     3901 agattttttt tttagtttgg tagcacattt tgtaccaaaa atgtcaaaca ctgtgctgta
     3961 agaatatcca tgtttgtaga aatgtccact tttcagataa tataatgcct accattatac
     4021 taacagaatc atatggtagt tgatttattt ttttatttat tatgtatatt tttggtattg
     4081 tgggttcttg aggcaatgat aaaaacactt aatgtattct gacatgagtg ctctaatagc
     4141 ctccttctcc tcatttttaa actgcataca ttactttcaa aataggtata gatactctgt
     4201 cccgcctttt gagctattag cctgttcctg tttccctttg tcacctaagc aaggcttttt
     4261 ctgagaaggt agtgaatggt ttcaaatgtt gcatactata agaataatca ttgggtaact
     4321 gttgtttaga ccaacactta gaaatactat atttgtgcct tttcattttt aattttaatg
     4381 tgtgttgata tttggagcac aaataatgaa ggtgccataa tatggcttgc caatgttacc
     4441 tccttgaata gtcatgtgtc attgtcttga attggtaatt ggagaacctt gcatgaaata
     4501 tgtgatcgtg tgtgtgtgtg tgtatgcgcg tgtgtgtctg tgtgtttgtg tacatacctg
     4561 tatttgcttg gggcttgtgt gtggtatgtt acaaagagtg aatttctgga aatagaaatc
     4621 agttaaatgt tgaaagttca ggttagcaga aatatttcat ttaaatatgc tttactttgg
     4681 aggacaattg attaacagag gaaatgataa ttttcaaaaa tgtgatcaat ttactgcatg
     4741 atgaaatgtg aaaacagtgc ctttttagga catcaattat aataaaattg ttttaaaata
     4801 ttaacaaaga tctcaaaaag ttgtcatgaa cattattcat ttatttttaa actggatcta
     4861 aataagagct tagatggcca aaattagaat taataatata cccatttaaa ttttgttcat
     4921 aaatttaaaa tcttaatcag aattctttat aaaatgtggg tcatagatat gacccagtgt
     4981 tactaaaata gaacagggat tgtgaaaatc cagctcaaca tacttaagta tacttggctt
     5041 agagccaagt atacttgaag aggtgaatta ttctgacttg gacatgcatg ctctttgatg
     5101 gataaaataa aaatattcaa tttattctta caaaagaagg tgggtgggtg agtgggttcg
     5161 ttttagtgtt ctcagattat aaagacagct ataaagacag cactttccgc acacaaagtg
     5221 tattttacaa acctttttta tacaaattaa tgagctctac tttatttaag tgttcatgga
     5281 atgatgtaaa ttttaggtcc agttgaacaa atattgagtg cctatcatat gcaagactaa
     5341 ctccttacta ggaatgaaat cacacagtgt cttctgtttg cagtatgtga actttatgtt
     5401 tgaaaagaaa ttattatatt taaatttttt tgttgtcaga gtttatcatt gtatactgta
     5461 acccagtaaa ttttgcattc agttttaaaa aatgaagatg taacttacct gagtctcatt
     5521 tttgaaaatg aaattctgca aaaattattt aaaaattagt tcttggggaa attgattttc
     5581 aagattcaag tgtataaaaa cttatattga acttttcagc ctcgttttta attagctgat
     5641 gttaatgata agatacataa tacatgtatc ttgttgctga aaatattttt tgcatttcaa
     5701 cacattgagt taaaataaag ttgttactac ttattcaaga ttaattggct tatcttgtgt
     5761 ttatttgaag aatacatact cttctttttc aggttggtat gatgctttgc aaaagagcca
     5821 tgtatttgat acggtaaatc atgcatagta attgtctttt ctacataggg gagctcttgt
     5881 tttttggaac caattgaaga cattcttaac tgtaaaatat taatatgcat ttattttaat
     5941 atgtatgaac catcaatact ggatgactta ttctaatgct acgtaccatt aatgtaacag
     6001 agatcacctt ttccctgtca ttcctatcct gtcttaattt cagttatccc agacaa
//
