LOCUS       NM_012445               1579 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens spondin 2 (SPON2), transcript variant 1, mRNA.
ACCESSION   NM_012445
VERSION     NM_012445.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Tang,J., Huang,Q., Li,X. and Gu,S.
  TITLE     Comprehensive analysis of the oncogenic and immunological role of
            SPON2 in human tumors
  JOURNAL   Medicine (Baltimore) 102 (37), e35122 (2023)
   PUBMED   37713832
  REMARK    GeneRIF: Comprehensive analysis of the oncogenic and immunological
            role of SPON2 in human tumors.
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Martins,A.L.M.D.S., Bernardes,A.B., Ferreira,V.A., Wanderley,D.C.,
            Araujo,S.A., do Carmo Neto,J.R., da Silva,C.A., Lira,R.C.P.,
            Araujo,L.S., Dos Reis,M.A. and Machado,J.R.
  TITLE     In situ assessment of Mindin as a biomarker of podocyte lesions in
            diabetic nephropathy
  JOURNAL   PLoS One 18 (5), e0284789 (2023)
   PUBMED   37130106
  REMARK    GeneRIF: In situ assessment of Mindin as a biomarker of podocyte
            lesions in diabetic nephropathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1579)
  AUTHORS   Wu,M., Kong,D. and Zhang,Y.
  TITLE     SPON2 promotes the bone metastasis of lung adenocarcinoma via
            activation of the NF-kappaB signaling pathway
  JOURNAL   Bone 167, 116630 (2023)
   PUBMED   36427776
  REMARK    GeneRIF: SPON2 promotes the bone metastasis of lung adenocarcinoma
            via activation of the NF-kappaB signaling pathway.
REFERENCE   4  (bases 1 to 1579)
  AUTHORS   Wang,H., Zhang,M., Lu,W. and Yuan,C.
  TITLE     Prostate cancer cell-derived spondin 2 boosts osteogenic factor
            levels in osteoblasts via the PI3K/AKT/mTOR pathway
  JOURNAL   Oncol Rep 49 (1) (2023)
   PUBMED   36484410
  REMARK    GeneRIF: Prostate cancer cellderived spondin 2 boosts osteogenic
            factor levels in osteoblasts via the PI3K/AKT/mTOR pathway.
REFERENCE   5  (bases 1 to 1579)
  AUTHORS   Huang,C., Ou,R., Chen,X., Zhang,Y., Li,J., Liang,Y., Zhu,X.,
            Liu,L., Li,M., Lin,D., Qiu,J., Liu,G., Zhang,L., Wu,Y., Tang,H.,
            Liu,Y., Liang,L., Ding,Y. and Liao,W.
  TITLE     Tumor cell-derived SPON2 promotes M2-polarized tumor-associated
            macrophage infiltration and cancer progression by activating PYK2
            in CRC
  JOURNAL   J Exp Clin Cancer Res 40 (1), 304 (2021)
   PUBMED   34583750
  REMARK    GeneRIF: Tumor cell-derived SPON2 promotes M2-polarized
            tumor-associated macrophage infiltration and cancer progression by
            activating PYK2 in CRC.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1579)
  AUTHORS   Li,Y., Cao,C., Jia,W., Yu,L., Mo,M., Wang,Q., Huang,Y., Lim,J.M.,
            Ishihara,M., Wells,L., Azadi,P., Robinson,H., He,Y.W., Zhang,L. and
            Mariuzza,R.A.
  TITLE     Structure of the F-spondin domain of mindin, an integrin ligand and
            pattern recognition molecule
  JOURNAL   EMBO J 28 (3), 286-297 (2009)
   PUBMED   19153605
  REMARK    GeneRIF: The structure of the F-spondin domain of mindin was
            determined at 1.8-A resolution.
REFERENCE   7  (bases 1 to 1579)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   8  (bases 1 to 1579)
  AUTHORS   Feinstein,Y. and Klar,A.
  TITLE     The neuronal class 2 TSR proteins F-spondin and Mindin: a small
            family with divergent biological activities
  JOURNAL   Int J Biochem Cell Biol 36 (6), 975-980 (2004)
   PUBMED   15094111
  REMARK    Review article
REFERENCE   9  (bases 1 to 1579)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   10 (bases 1 to 1579)
  AUTHORS   Manda,R., Kohno,T., Matsuno,Y., Takenoshita,S., Kuwano,H. and
            Yokota,J.
  TITLE     Identification of genes (SPON2 and C20orf2) differentially
            expressed between cancerous and noncancerous lung cells by mRNA
            differential display
  JOURNAL   Genomics 61 (1), 5-14 (1999)
   PUBMED   10512675
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092535.4.
            
            On Nov 23, 2018 this sequence version replaced NM_012445.3.
            
            Transcript Variant: This variant (1) encodes the same protein as
            variants 2 and 3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358948.1, SRR3476690.188311.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000290902.10/ ENSP00000290902.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                AC092535.4         73316-73355         c
            41-263              AC092535.4         72624-72846         c
            264-487             AC092535.4         72035-72258         c
            488-679             AC092535.4         71771-71962         c
            680-854             AC092535.4         71174-71348         c
            855-1579            AC092535.4         67704-68428         c
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p16.3"
     gene            1..1579
                     /gene="SPON2"
                     /gene_synonym="DIL-1; DIL1; M-SPONDIN; MINDIN"
                     /note="spondin 2"
                     /db_xref="GeneID:10417"
                     /db_xref="HGNC:HGNC:11253"
                     /db_xref="MIM:605918"
     exon            1..40
                     /gene="SPON2"
                     /gene_synonym="DIL-1; DIL1; M-SPONDIN; MINDIN"
                     /inference="alignment:Splign:2.1.0"
     exon            41..263
                     /gene="SPON2"
                     /gene_synonym="DIL-1; DIL1; M-SPONDIN; MINDIN"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..1039
                     /gene="SPON2"
                     /gene_synonym="DIL-1; DIL1; M-SPONDIN; MINDIN"
                     /note="differentially expressed in cancerous and
                     non-cancerous lung cells 1; spondin 2, extracellular
                     matrix protein"
                     /codon_start=1
                     /product="spondin-2 precursor"
                     /protein_id="NP_036577.2"
                     /db_xref="GeneID:10417"
                     /db_xref="HGNC:HGNC:11253"
                     /db_xref="MIM:605918"
                     /translation="MENPSPAAALGKALCALLLATLGAAGQPLGGESICSARALAKYS
                     ITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGE
                     AWALMKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPD
                     WFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSP
                     SHPANSFYYPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPL
                     DCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGSPCPELEEEAECVPDNCV"
     exon            264..487
                     /gene="SPON2"
                     /gene_synonym="DIL-1; DIL1; M-SPONDIN; MINDIN"
                     /inference="alignment:Splign:2.1.0"
     exon            488..679
                     /gene="SPON2"
                     /gene_synonym="DIL-1; DIL1; M-SPONDIN; MINDIN"
                     /inference="alignment:Splign:2.1.0"
     exon            680..854
                     /gene="SPON2"
                     /gene_synonym="DIL-1; DIL1; M-SPONDIN; MINDIN"
                     /inference="alignment:Splign:2.1.0"
     exon            855..1579
                     /gene="SPON2"
                     /gene_synonym="DIL-1; DIL1; M-SPONDIN; MINDIN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1552..1557
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPON2"
                     /gene_synonym="DIL-1; DIL1; M-SPONDIN; MINDIN"
                     /note="hexamer: AATAAA"
     polyA_site      1579
                     /gene="SPON2"
                     /gene_synonym="DIL-1; DIL1; M-SPONDIN; MINDIN"
                     /note="major polyA site"
ORIGIN      
        1 acccgaccgc tgccggccgc gctcccgctg ctcctgccgg gtgatggaaa accccagccc
       61 ggccgccgcc ctgggcaagg ccctctgcgc tctcctcctg gccactctcg gcgccgccgg
      121 ccagcctctt gggggagagt ccatctgttc cgccagagcc ctggccaaat acagcatcac
      181 cttcacgggc aagtggagcc agacggcctt ccccaagcag taccccctgt tccgcccccc
      241 tgcgcagtgg tcttcgctgc tgggggccgc gcatagctcc gactacagca tgtggaggaa
      301 gaaccagtac gtcagtaacg ggctgcgcga ctttgcggag cgcggcgagg cctgggcgct
      361 gatgaaggag atcgaggcgg cgggggaggc gctgcagagc gtgcacgcgg tgttttcggc
      421 gcccgccgtc cccagcggca ccgggcagac gtcggcggag ctggaggtgc agcgcaggca
      481 ctcgctggtc tcgtttgtgg tgcgcatcgt gcccagcccc gactggttcg tgggcgtgga
      541 cagcctggac ctgtgcgacg gggaccgttg gcgggaacag gcggcgctgg acctgtaccc
      601 ctacgacgcc gggacggaca gcggcttcac cttctcctcc cccaacttcg ccaccatccc
      661 gcaggacacg gtgaccgaga taacgtcctc ctctcccagc cacccggcca actccttcta
      721 ctacccgcgg ctgaaggccc tgcctcccat cgccagggtg acactggtgc ggctgcgaca
      781 gagccccagg gccttcatcc ctcccgcccc agtcctgccc agcagggaca atgagattgt
      841 agacagcgcc tcagttccag aaacgccgct ggactgcgag gtctccctgt ggtcgtcctg
      901 gggactgtgc ggaggccact gtgggaggct cgggaccaag agcaggactc gctacgtccg
      961 ggtccagccc gccaacaacg ggagcccctg ccccgagctc gaagaagagg ctgagtgcgt
     1021 ccctgataac tgcgtctaag accagagccc cgcagcccct ggggcccccc ggagccatgg
     1081 ggtgtcgggg gctcctgtgc aggctcatgc tgcaggcggc cgagggcaca gggggtttcg
     1141 cgctgctcct gaccgcggtg aggccgcgcc gaccatctct gcactgaagg gccctctggt
     1201 ggccggcacg ggcattggga aacagcctcc tcctttccca accttgcttc ttaggggccc
     1261 ccgtgtcccg tctgctctca gcctcctcct cctgcaggat aaagtcatcc ccaaggctcc
     1321 agctactcta aattatgtct ccttataagt tattgctgct ccaggagatt gtccttcatc
     1381 gtccaggggc ctggctccca cgtggttgca gatacctcag acctggtgct ctaggctgtg
     1441 ctgagcccac tctcccgagg gcgcatccaa gcgggggcca cttgagaagt gaataaatgg
     1501 ggcggtttcg gaagcgtcag tgtttccatg ttatggatct ctctgcgttt gaataaagac
     1561 tatctctgtt gctcacaaa
//
