LOCUS       NM_001244950            5224 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens SPARC (osteonectin), cwcv and kazal like domains
            proteoglycan 2 (SPOCK2), transcript variant 3, mRNA.
ACCESSION   NM_001244950
VERSION     NM_001244950.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Xiao,M., Xue,J. and Jin,E.
  TITLE     SPOCK: Master regulator of malignant tumors (Review)
  JOURNAL   Mol Med Rep 30 (6) (2024)
   PUBMED   39392048
  REMARK    GeneRIF: SPOCK: Master regulator of malignant tumors (Review).
            Review article
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Kang,J.Y., Cho,H., Gil,M., Lee,H., Park,S. and Kim,K.E.
  TITLE     The novel prognostic marker SPOCK2 regulates tumour progression in
            melanoma
  JOURNAL   Exp Dermatol 33 (6), e15092 (2024)
   PUBMED   38888196
  REMARK    GeneRIF: The novel prognostic marker SPOCK2 regulates tumour
            progression in melanoma.
REFERENCE   3  (bases 1 to 5224)
  AUTHORS   Aghamaliyev,U., Su,K., Weniger,M., Koch,D., D'Haese,J.G., Werner,J.
            and Bazhin,A.V.
  TITLE     SPOCK2 gene expression is downregulated in pancreatic ductal
            adenocarcinoma cells and correlates with prognosis of patients with
            pancreatic cancer
  JOURNAL   J Cancer Res Clin Oncol 149 (11), 9191-9200 (2023)
   PUBMED   37188984
  REMARK    GeneRIF: SPOCK2 gene expression is downregulated in pancreatic
            ductal adenocarcinoma cells and correlates with prognosis of
            patients with pancreatic cancer.
REFERENCE   4  (bases 1 to 5224)
  AUTHORS   Tan,Z., Li,F., Chen,Q., Chen,H., Xue,Z., Zhang,J., Gao,Y.,
            Liang,L., Huang,T., Zhang,S., Li,J., Shu,Q. and Yu,L.
  TITLE     Integrated bulk and single-cell RNA-sequencing reveals SPOCK2 as a
            novel biomarker gene in the development of congenital pulmonary
            airway malformation
  JOURNAL   Respir Res 24 (1), 127 (2023)
   PUBMED   37165378
  REMARK    GeneRIF: Integrated bulk and single-cell RNA-sequencing reveals
            SPOCK2 as a novel biomarker gene in the development of congenital
            pulmonary airway malformation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5224)
  AUTHORS   David,S.P., Hamidovic,A., Chen,G.K., Bergen,A.W., Wessel,J.,
            Kasberger,J.L., Brown,W.M., Petruzella,S., Thacker,E.L., Kim,Y.,
            Nalls,M.A., Tranah,G.J., Sung,Y.J., Ambrosone,C.B., Arnett,D.,
            Bandera,E.V., Becker,D.M., Becker,L., Berndt,S.I., Bernstein,L.,
            Blot,W.J., Broeckel,U., Buxbaum,S.G., Caporaso,N., Casey,G.,
            Chanock,S.J., Deming,S.L., Diver,W.R., Eaton,C.B., Evans,D.S.,
            Evans,M.K., Fornage,M., Franceschini,N., Harris,T.B.,
            Henderson,B.E., Hernandez,D.G., Hitsman,B., Hu,J.J., Hunt,S.C.,
            Ingles,S.A., John,E.M., Kittles,R., Kolb,S., Kolonel,L.N., Le
            Marchand,L., Liu,Y., Lohman,K.K., McKnight,B., Millikan,R.C.,
            Murphy,A., Neslund-Dudas,C., Nyante,S., Press,M., Psaty,B.M.,
            Rao,D.C., Redline,S., Rodriguez-Gil,J.L., Rybicki,B.A.,
            Signorello,L.B., Singleton,A.B., Smoller,J., Snively,B., Spring,B.,
            Stanford,J.L., Strom,S.S., Swan,G.E., Taylor,K.D., Thun,M.J.,
            Wilson,A.F., Witte,J.S., Yamamura,Y., Yanek,L.R., Yu,K., Zheng,W.,
            Ziegler,R.G., Zonderman,A.B., Jorgenson,E., Haiman,C.A. and
            Furberg,H.
  TITLE     Genome-wide meta-analyses of smoking behaviors in African Americans
  JOURNAL   Transl Psychiatry 2 (5), e119 (2012)
   PUBMED   22832964
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5224)
  AUTHORS   Hadchouel,A., Durrmeyer,X., Bouzigon,E., Incitti,R., Huusko,J.,
            Jarreau,P.H., Lenclen,R., Demenais,F., Franco-Montoya,M.L.,
            Layouni,I., Patkai,J., Bourbon,J., Hallman,M., Danan,C. and
            Delacourt,C.
  TITLE     Identification of SPOCK2 as a susceptibility gene for
            bronchopulmonary dysplasia
  JOURNAL   Am J Respir Crit Care Med 184 (10), 1164-1170 (2011)
   PUBMED   21836138
  REMARK    GeneRIF: candidate susceptibility gene for bronchopulmonary
            dysplasia; lung expression pattern indicates potential role in
            alveolarization
REFERENCE   7  (bases 1 to 5224)
  AUTHORS   Pavsic,M., Vito,T. and Lenarcic,B.
  TITLE     Purification and characterization of a recombinant human testican-2
            expressed in baculovirus-infected Sf9 insect cells
  JOURNAL   Protein Expr Purif 58 (1), 132-139 (2008)
   PUBMED   17950619
  REMARK    GeneRIF: purification and characterization of a recombinant
            testican-2 expressed in insect cells; the C1q subcomponent of
            complement component C1 was identified as a potential interacting
            partner of testican-2
REFERENCE   8  (bases 1 to 5224)
  AUTHORS   Zhang,Z. and Henzel,W.J.
  TITLE     Signal peptide prediction based on analysis of experimentally
            verified cleavage sites
  JOURNAL   Protein Sci 13 (10), 2819-2824 (2004)
   PUBMED   15340161
REFERENCE   9  (bases 1 to 5224)
  AUTHORS   Nakada,M., Miyamori,H., Yamashita,J. and Sato,H.
  TITLE     Testican 2 abrogates inhibition of membrane-type matrix
            metalloproteinases by other testican family proteins
  JOURNAL   Cancer Res 63 (12), 3364-3369 (2003)
   PUBMED   12810672
  REMARK    GeneRIF: Neurons are a major source of all of the testican family
            members in the normal brain. Abundant distribution of testican 2
            may contribute to glioma invasion by inactivating other testican
            family members including N-Tes, which all inhibit MT-MMPs.
REFERENCE   10 (bases 1 to 5224)
  AUTHORS   Vannahme,C., Schubel,S., Herud,M., Gosling,S., Hulsmann,H.,
            Paulsson,M., Hartmann,U. and Maurer,P.
  TITLE     Molecular cloning of testican-2: defining a novel calcium-binding
            proteoglycan family expressed in brain
  JOURNAL   J Neurochem 73 (1), 12-20 (1999)
   PUBMED   10386950
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022392.5, BC023558.2 and
            BQ029472.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001244950.1.
            
            Summary: This gene encodes a protein which binds with
            glycosaminoglycans to form part of the extracellular matrix. The
            protein contains thyroglobulin type-1, follistatin-like, and
            calcium-binding domains, and has glycosaminoglycan attachment sites
            in the acidic C-terminal region. Three alternatively spliced
            transcript variants that encode different protein isoforms have
            been described for this gene. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (3) has a different 5' UTR,
            compared to variant 2. Variants 2 and 3 encode the same protein
            (isoform 2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.43002.1,
                                           SRR1803611.44498.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373109.7/ ENSP00000362201.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-412               AC022392.5         106358-106769       c
            413-1672            BC023558.2         629-1888
            1673-4672           AC022392.5         77804-80803         c
            4673-5224           BQ029472.1         18-569              c
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.1"
     gene            1..5224
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /note="SPARC (osteonectin), cwcv and kazal like domains
                     proteoglycan 2"
                     /db_xref="GeneID:9806"
                     /db_xref="HGNC:HGNC:13564"
                     /db_xref="MIM:607988"
     exon            1..412
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             224..1498
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 3; SPARC/osteonectin, CWCV, and Kazal-like domains
                     proteoglycan 2; SPARC/osteonectin, cwcv and kazal like
                     domains proteoglycan 2; sparc/osteonectin, cwcv and
                     kazal-like domains proteoglycan (testican) 2"
                     /codon_start=1
                     /product="testican-2 isoform 2 precursor"
                     /protein_id="NP_001231879.1"
                     /db_xref="CCDS:CCDS7313.1"
                     /db_xref="GeneID:9806"
                     /db_xref="HGNC:HGNC:13564"
                     /db_xref="MIM:607988"
                     /translation="MRAPGCGRLVLPLLLLAAAALAEGDAKGLKEGETPGNFMEDEQW
                     LSSISQYSGKIKHWNRFRDEVEDDYIKSWEDNQQGDEALDTTKDPCQKVKCSRHKVCI
                     AQGYQRAMCISRKKLEHRIKQPTVKLHGNKDSICKPCHMAQLASVCGSDGHTYSSVCK
                     LEQQACLSSKQLAVRCEGPCPCPTEQAATSTADGKPETCTGQDLADLGDRLRDWFQLL
                     HENSKQNGSASSVAGPASGLDKSLGASCKDSIGWMFSKLDTSADLFLDQTELAAINLD
                     KYEVCIRPFFNSCDTYKDGRVSTAEWCFCFWREKPPCLAELERIQIQEAAKKKPGIFI
                     PSCDEDGYYRKMQCDQSSGDCWCVDQLGLELTGTRTHGSPDCDDIVGFSGDFGSGVGW
                     EDEEEKETEEAGEEAEEEEGEAGEADDGGYIW"
     exon            413..421
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     exon            422..467
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     exon            468..582
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     exon            583..697
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     exon            698..812
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     exon            813..932
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1151
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1214
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1352
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..5224
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5203..5208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /note="hexamer: AATAAA"
     polyA_site      5224
                     /gene="SPOCK2"
                     /gene_synonym="testican-2"
                     /note="major polyA site"
ORIGIN      
        1 gattctctct ccctctccgc gtccagtgct gggctttttc agacaagtgc atctcctaac
       61 caggtcacat ttcagccgcg acccactctc cgccagtcac cggaggcaga ccgcgggagg
      121 agagctgagg acagccgcgt gcgcttcgcc agcagcgggg tgggaggaag gacattaaaa
      181 tactgcagaa gtcaagaccc ccccaggtcg aacccagacc acgatgcgcg ccccgggctg
      241 cgggcggctg gtgctgccgc tgctgctcct ggccgcggca gccctggccg aaggcgacgc
      301 caaggggctc aaggagggcg agacccccgg caatttcatg gaggacgagc aatggctgtc
      361 gtccatctcg cagtacagcg gcaagatcaa gcactggaac cgcttccgag acgaagtgga
      421 ggatgactat atcaagagct gggaggacaa tcagcaagga gatgaagccc tggataccac
      481 caaggacccc tgccagaagg tgaagtgcag ccgccacaag gtgtgcattg cccagggcta
      541 ccagcgggcc atgtgcatca gtcgcaagaa gctggagcac aggatcaagc agccgaccgt
      601 gaaactccat ggaaacaaag actccatctg caagccctgc cacatggccc agcttgcctc
      661 tgtctgcggc tcagatggcc acacttacag ctctgtgtgt aagctggagc aacaggcgtg
      721 cctgagcagc aagcagctgg cggtgcgatg cgagggcccc tgcccctgcc ccacggagca
      781 ggctgccacc tccaccgccg atggcaaacc agagacttgc accggtcagg acctggctga
      841 cctgggagat cggctgcggg actggttcca gctccttcat gagaactcca agcagaatgg
      901 ctcagccagc agtgtagccg gcccggccag cgggctggac aagagcctgg gggccagctg
      961 caaggactcc attggctgga tgttctccaa gctggacacc agtgctgacc tcttcctgga
     1021 ccagacggag ctggccgcca tcaacctgga caagtacgag gtctgcatcc gtcccttctt
     1081 caactcctgt gacacctaca aggatggccg ggtctctact gctgagtggt gcttctgctt
     1141 ctggagggag aagcccccct gcctggcaga gctggagcgc atccagatcc aggaggccgc
     1201 caagaagaag ccaggcatct tcatcccgag ctgcgacgag gatggctact accggaagat
     1261 gcagtgtgac cagagcagcg gtgactgctg gtgtgtggac cagctgggcc tggagctgac
     1321 tggcacgcgc acgcatggga gccccgactg cgatgacatc gtgggcttct cgggggactt
     1381 tggaagcggt gtcggctggg aggatgagga ggagaaggag acggaggaag caggcgagga
     1441 ggccgaggag gaggagggcg aggcaggcga ggctgacgac gggggctaca tctggtagac
     1501 gccctcagga gccggctgcc gggggggact caacagcaga gctctgagca gcagcaggca
     1561 acttcgagaa cggatccaga aatgcagtca gaaggaccct gctccacctg gggggactgg
     1621 gagtgtgagt gtgcatggca tgtgtgtggc acagatggct gggacgggtg acagtgtgag
     1681 tgcatgtgtg catgcatgtg tgtatgtgtg tgtgtgtgtg gcatgcgctg acaaatgtgt
     1741 ccttgatcca cactgctcct ggcagagtga gtcacccaaa ggccccttcg gcctccttgt
     1801 agctgttttc tttccttttg ttgttggttt taaaatacat tcacacacaa atacaaattg
     1861 acaggtcaaa atccatgaaa tgagatcccc cagccgtgtc ctccagccca gccctgaccc
     1921 cttggtttct accctggctc cccttggttt ctaccctggc tcaaccgacc cctgtctgcc
     1981 cttctccctc ttgcttctga ggtcaagctc tggcctgcga gcctgtcccc attgcaaagg
     2041 ggagggaggg gcagggagct gtctaccagc tgaggtcctc ccaaaactgg gccgatgtgg
     2101 tgtgacatcc ccaccagcct cagatgagac gggccaggac gcccagccac agcaagccct
     2161 gtccctttgc cggatcccca aacactagag aagctctcct aacccaaggc ggagaatgaa
     2221 ggtggtggcg gcagaggagg agggcagcag ctgagaggcc agggacaggg tgcctcgcca
     2281 agctgtctga ggtctgtccc aggtggccca ggtggtgcag gtagaacagg gtgaggagag
     2341 ggggtcggct cagcaggagg aggctgtggc tgcagagcct gggggagctt ttaggtgttg
     2401 agatggggca gctctgaatc ctagaccctg gaatagcctg tcccttttct ctgggtctcg
     2461 tggtggagcc atgatctggg ctgctctctt ggggacactg ggtggtggtt acacagttga
     2521 cctctgcctg gctccccctt ggtgcaactc ctgcctccat cccccttgct ggggtcccct
     2581 catccacttg agggcgcctg agggccagga gcagcaggca aggagcctgg gtctaggcta
     2641 agggggtgtg tgcccacctc ctccctgacc cttaacactc ctgtcctgcc cagaccaaca
     2701 gagagagctg tccctgagac cccggagaga agcagctgcc gaaagctgca gcctttccgc
     2761 actctgagac catgatcttc ctcctgccag gggagagcca cccacaggcc atgtccagcc
     2821 ccacttccct cagcccccag ggcttccttc tggcccctct gaggattccc tagggctgcc
     2881 ccgcagaggg gcttccccaa gctctgtttt gaagcctgca atgtggaaaa gtgagaagtc
     2941 agagggaaca ggacaggtgc agccgggctc tgaggccaca cctcacacct cgctgttccc
     3001 caacatcccc tgagcagtgt gagctcatct caccagatga gaagaggccc tgtgcatttc
     3061 ttttgtttgt ttgttgctgt tttcccccac ccatccagtt ctcctcagca aagcaaattc
     3121 cttaacacct ttggtggaga atttcttacc cagacttggg gctgtgatgc ccttcagtgc
     3181 gtggtgagtg cagcgtgtgt gcgtgtgcct gtgtgtgaac ctgggggcca tcctggtggc
     3241 ctgggagcgt gaggagaggc cccctgtgtg ctgggtgagt ggtgggtgtg gggtcaatgc
     3301 agtgaggctc tctgggtgag gctcccaacc tggcagtccc cagcctccca gcatctgtga
     3361 gcgtctgttg gactttacag aagagcctca tcctgtctgc ccctcactct gccctggaat
     3421 caacatcttc cgagtccttc ttgggggaaa tagcagagcc ccacttaact ccataaactg
     3481 cttcccattc cgcagcccag ttctgattgt tgaggtgtcg cgtcgttcca ggtcccccag
     3541 tcccctcttt ctcctgtcct ctctctgtcc ttcacctccc cactccagcc ccggctcagt
     3601 tcagggaaat gctgttccac atcagccctc tgctctctga ggcagccgcg cctctgactc
     3661 ggagctactt gaaacttctg ctcttgctag gattggagtc tacctatctc ttccatttgt
     3721 cccagctgga gttctggaac tttcctcctc ggggtggggg tgggggttgt taaggatgct
     3781 ggggggcctg gggaaggaag gagttcagag gaagggtgtc ccctgtcctc ttgatgtcac
     3841 cctccgctcc tgggacacgt gctctctctg tctctgggtc ttctggctgt gcacgtttgt
     3901 gtgtccttgt aaatatgttt taggaagaaa gcaaaaggga ctgaactagc ctctggtagg
     3961 attgcagggg tccagccttg cctgtttccg aagcccccac actgcctttc gccctactga
     4021 gactggtccc ctcaaaaggt agacaaaaca gcagctccct gtggagctga agggcggcct
     4081 caaagtggct ttttgttaga caaggttaag gtttcctcat gagcaaggtt gcagatcggt
     4141 ccttcctcag ctccttgatt tgtgaccttg accaaggggc ctgccaccca gcccctccag
     4201 tgccctctcc tcgatgcctc gctccttcct gcccccactc ccctggctta ggcaggtagg
     4261 ggaattaggg ccatgctgga agaagcttaa ccatgtgttc aaagaacggt ttcttgcttg
     4321 cttggtcctg gaactcccct tggctgcccc aggcctcctt ggcccatggg tgctggggga
     4381 ggtggatgtc agatctggta ggttgcagca gagaaaataa atgtgccttg agagaccact
     4441 cagagagggt ccaagggtga tggagaagga agcatggcct gggagcttgg aagggagggg
     4501 tggtgggtgg cggcatcttg actgccccct gttgtcccac acgtgggggg tggtcacccc
     4561 ccttcactcc agcccgcctg ccttcagcct tccatgagct tcacctgctt ccaacttcac
     4621 tttggagggg gtggggtccg ttggcatcaa cacggggacc ctctgcttca ccaaagcccg
     4681 agccctcagc ccctggggag aacaaatggc tgagctttga tacctggggt cgtcgagagg
     4741 ctgcgggctg gcggcagtcc caggggagag acaccacaga aggagaccca gacatcccga
     4801 ggaagttccc agcagagcaa actgctttcc agcctgaagc ctgcttaaac tgtgtgatgt
     4861 gcaataactg agcttagagt taggaattgt gttcaagtgc ttggatttcc gtctgtagat
     4921 ttaactgctg aaattgtatc tctcagtaat tttagatgtc ttttaaaaaa ttgaaaaaca
     4981 aagtgttaga ctgtgtgcgt gtgcgttgat gggcactcaa gagtcccgtg agtcatccag
     5041 ccctgccttt cccctgcgcc cccatcctct cacgtcccgc cccgcctcca cttggggacc
     5101 ctgcctcgtg tcgtctttat ctgcctatta ctcagcctaa ggaaacaagt acactccaca
     5161 catgcataaa ggaaatcaaa tgttattttt aagaaaatgg aaaataaaaa ctttataaac
     5221 acca
//
