LOCUS       NP_004589                439 aa            linear   PRI 29-OCT-2024
DEFINITION  testican-1 precursor [Homo sapiens].
ACCESSION   NP_004589
VERSION     NP_004589.1
DBSOURCE    REFSEQ: accession NM_004598.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 439)
  AUTHORS   Xiao,M., Xue,J. and Jin,E.
  TITLE     SPOCK: Master regulator of malignant tumors (Review)
  JOURNAL   Mol Med Rep 30 (6) (2024)
   PUBMED   39392048
  REMARK    GeneRIF: SPOCK: Master regulator of malignant tumors (Review).
            Review article
REFERENCE   2  (residues 1 to 439)
  AUTHORS   Liu,Y., Han,T., Wu,J., Zhou,J., Guo,J., Miao,R., Xu,Z., Xing,Y.,
            Bai,Y. and Hu,D.
  TITLE     SPOCK1, as a potential prognostic and therapeutic biomarker for
            lung adenocarcinoma, is associated with epithelial-mesenchymal
            transition and immune evasion
  JOURNAL   J Transl Med 21 (1), 909 (2023)
   PUBMED   38087364
  REMARK    GeneRIF: SPOCK1, as a potential prognostic and therapeutic
            biomarker for lung adenocarcinoma, is associated with
            epithelial-mesenchymal transition and immune evasion.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 439)
  AUTHORS   Lin,Y.W., Wen,Y.C., Hsiao,C.H., Lai,F.R., Yang,S.F., Yang,Y.C.,
            Ho,K.H., Hsieh,F.K., Hsiao,M., Lee,W.J. and Chien,M.H.
  TITLE     Proteoglycan SPOCK1 as a Poor Prognostic Marker Promotes Malignant
            Progression of Clear Cell Renal Cell Carcinoma via Triggering the
            Snail/Slug-MMP-2 Axis-Mediated Epithelial-to-Mesenchymal Transition
  JOURNAL   Cells 12 (3), 352 (2023)
   PUBMED   36766694
  REMARK    GeneRIF: Proteoglycan SPOCK1 as a Poor Prognostic Marker Promotes
            Malignant Progression of Clear Cell Renal Cell Carcinoma via
            Triggering the Snail/Slug-MMP-2 Axis-Mediated
            Epithelial-to-Mesenchymal Transition.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 439)
  AUTHORS   Gan,C., Li,M., Lu,Y., Peng,G., Li,W., Wang,H., Peng,Y., Hu,Q.,
            Wei,W., Wang,F., Liu,L. and Zhao,Q.
  TITLE     SPOCK1 and POSTN are valuable prognostic biomarkers and correlate
            with tumor immune infiltrates in colorectal cancer
  JOURNAL   BMC Gastroenterol 23 (1), 4 (2023)
   PUBMED   36611136
  REMARK    GeneRIF: SPOCK1 and POSTN are valuable prognostic biomarkers and
            correlate with tumor immune infiltrates in colorectal cancer.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 439)
  AUTHORS   Qu,Y.L., Liu,X.L., Zhao,S.Y. and Zhai,X.F.
  TITLE     SPOCK1 silencing decreases 5-FU resistance through PRRX1 in
            colorectal cancer
  JOURNAL   Pathol Res Pract 234, 153895 (2022)
   PUBMED   35462225
  REMARK    GeneRIF: SPOCK1 silencing decreases 5-FU resistance through PRRX1
            in colorectal cancer.
REFERENCE   6  (residues 1 to 439)
  AUTHORS   Marr,H.S., Basalamah,M.A. and Edgell,C.J.
  TITLE     Endothelial cell expression of testican mRNA
  JOURNAL   Endothelium 5 (3), 209-219 (1997)
   PUBMED   9272383
REFERENCE   7  (residues 1 to 439)
  AUTHORS   Charbonnier,F., Perin,J.P., Roussel,G., Nussbaum,J.L. and
            Alliel,P.M.
  TITLE     [Cloning of testican/SPOCK in man and mouse. Neuromuscular
            expression perspectives in pathology]
  JOURNAL   C R Seances Soc Biol Fil 191 (1), 127-133 (1997)
   PUBMED   9181134
  REMARK    Review article
REFERENCE   8  (residues 1 to 439)
  AUTHORS   Bonnet,F., Perin,J.P., Charbonnier,F., Camuzat,A., Roussel,G.,
            Nussbaum,J.L. and Alliel,P.M.
  TITLE     Structure and cellular distribution of mouse brain testican.
            Association with the postsynaptic area of hippocampus pyramidal
            cells
  JOURNAL   J Biol Chem 271 (8), 4373-4380 (1996)
   PUBMED   8626787
REFERENCE   9  (residues 1 to 439)
  AUTHORS   Perin,J.P., Alliel,P.M., Jolles,P. and Bonnet,F.
  TITLE     Proteoglycans in male reproductive tract
  JOURNAL   EXS 70, 191-197 (1994)
   PUBMED   7507743
  REMARK    Review article
REFERENCE   10 (residues 1 to 439)
  AUTHORS   Alliel,P.M., Perin,J.P., Jolles,P. and Bonnet,F.J.
  TITLE     Testican, a multidomain testicular proteoglycan resembling
            modulators of cell social behaviour
  JOURNAL   Eur J Biochem 214 (1), 347-350 (1993)
   PUBMED   8389704
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI550122.1, AF231124.1,
            AC005213.1 and BC030691.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes the protein core of a seminal plasma
            proteoglycan containing chondroitin- and heparan-sulfate chains.
            The protein's function is unknown, although similarity to
            thyropin-type cysteine protease-inhibitors suggests its function
            may be related to protease inhibition. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC030691.2, AF231124.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394945.6/ ENSP00000378401.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.2"
     Protein         1..439
                     /product="testican-1 precursor"
                     /note="sparc/osteonectin, cwcv and kazal-like domains
                     proteoglycan (testican) 1; SPARC/osteonectin, cwcv and
                     kazal like domains proteoglycan 1"
                     /calculated_mol_wt=48993
     mat_peptide     22..439
                     /product="testican-1"
                     /calculated_mol_wt=47009
     Region          136..174
                     /region_name="Kazal_2"
                     /note="Kazal-type serine protease inhibitor domain;
                     pfam07648"
                     /db_xref="CDD:400135"
     Region          198..309
                     /region_name="EFh_SPARC_TICN1"
                     /note="EF-hand, extracellular calcium-binding (EC) motif,
                     found in testican-1 (TICN1); cd16237"
                     /db_xref="CDD:320016"
     Site            228
                     /site_type="glycosylation"
                     /note="O-linked (GalNAc...) threonine.
                     /evidence=ECO:0000269|PubMed:23234360; propagated from
                     UniProtKB/Swiss-Prot (Q08629.1)"
     Region          245..276
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320016"
     Site            order(257,261,268)
                     /site_type="other"
                     /note="putative Ca binding site [ion binding]"
                     /db_xref="CDD:320016"
     Region          277..309
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320016"
     Region          312..372
                     /region_name="TY"
                     /note="Thyroglobulin type I repeats.; The N-terminal
                     region of human thyroglobulin contains 11 type-1 repeats
                     TY repeats are proposed to be inhibitors of cysteine
                     proteases; cd00191"
                     /db_xref="CDD:238114"
     Site            order(317,332,350,354)
                     /site_type="active"
                     /note="protease interaction site [active]"
                     /db_xref="CDD:238114"
     Site            383
                     /site_type="glycosylation"
                     /note="O-linked (Xyl...) (glycosaminoglycan) serine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q08629.1)"
     Site            388
                     /site_type="glycosylation"
                     /note="O-linked (Xyl...) (glycosaminoglycan) serine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q08629.1)"
     Region          415..439
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q08629.1)"
     CDS             1..439
                     /gene="SPOCK1"
                     /gene_synonym="SPOCK; TESTICAN; TIC1"
                     /coded_by="NM_004598.4:149..1468"
                     /db_xref="CCDS:CCDS4191.1"
                     /db_xref="GeneID:6695"
                     /db_xref="HGNC:HGNC:11251"
                     /db_xref="MIM:602264"
ORIGIN      
        1 mpaiavlaaa aaawcflqve srhldalagg agpnhgnfld ndqwlstvsq ydrdkywnrf
       61 rdddyfrnwn pnkpfdqald pskdpclkvk csphkvcvtq dyqtalcvsr khllprqkkg
      121 nvaqkhwvgp snlvkckpcp vaqsamvcgs dghsytskck lefhacstgk slatlcdgpc
      181 pclpepeppk hkaersactd kelrnlasrl kdwfgalhed anrvikptss ntaqgrfdts
      241 ilpickdslg wmfnkldmny dllldpsein aiyldkyepc ikplfnscds fkdgklsnne
      301 wcycfqkpgg lpcqnemnri qklskgksll gafiprcnee gyykatqchg stgqcwcvdk
      361 ygnelagsrk qgavsceeeq etsgdfgsgg svvllddley erelgpkdke gklrvhtrav
      421 teddededdd kedevgyiw
//
