LOCUS       NM_003123               7252 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens sialophorin (SPN), transcript variant 2, mRNA.
ACCESSION   NM_003123
VERSION     NM_003123.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Vega-Mendoza,D., Canas-Linares,A., Flores-Alcantar,A.,
            Espinosa-Neira,R., Melchy-Perez,E., Vera-Estrella,R., Auvynet,C.
            and Rosenstein,Y.
  TITLE     CD43 (sialophorin) is involved in the induction of extracellular
            matrix remodeling and angiogenesis by lung cancer cells
  JOURNAL   J Cell Physiol 236 (9), 6643-6656 (2021)
   PUBMED   33533043
  REMARK    GeneRIF: CD43 (sialophorin) is involved in the induction of
            extracellular matrix remodeling and angiogenesis by lung cancer
            cells.
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Yoshimura,A., Asahina,Y., Chang,L.Y., Angata,T., Tanaka,H.,
            Kitajima,K. and Sato,C.
  TITLE     Identification and functional characterization of a Siglec-7
            counter-receptor on K562 cells
  JOURNAL   J Biol Chem 296, 100477 (2021)
   PUBMED   33640457
  REMARK    GeneRIF: Identification and functional characterization of a
            Siglec-7 counter-receptor on K562 cells.
REFERENCE   3  (bases 1 to 7252)
  AUTHORS   Sorigue,M.
  TITLE     CD43 in the malignant flow cytometry laboratory in 2020
  JOURNAL   Expert Rev Hematol 14 (1), 123-136 (2021)
   PUBMED   33249940
  REMARK    GeneRIF: CD43 in the malignant flow cytometry laboratory in 2020.
REFERENCE   4  (bases 1 to 7252)
  AUTHORS   Rogaev,E.I. and Keryanov,S.A.
  TITLE     Unusual variability of the complex dinucleotide repeat block at the
            SPN locus
  JOURNAL   Hum Mol Genet 1 (8), 657 (1992)
   PUBMED   1301183
REFERENCE   5  (bases 1 to 7252)
  AUTHORS   Schmid,K., Hediger,M.A., Brossmer,R., Collins,J.H., Haupt,H.,
            Marti,T., Offner,G.D., Schaller,J., Takagaki,K., Walsh,M.T. et al.
  TITLE     Amino acid sequence of human plasma galactoglycoprotein: identity
            with the extracellular region of CD43 (sialophorin)
  JOURNAL   Proc Natl Acad Sci U S A 89 (2), 663-667 (1992)
   PUBMED   1731338
REFERENCE   6  (bases 1 to 7252)
  AUTHORS   Rosenstein,Y., Park,J.K., Hahn,W.C., Rosen,F.S., Bierer,B.E. and
            Burakoff,S.J.
  TITLE     CD43, a molecule defective in Wiskott-Aldrich syndrome, binds
            ICAM-1
  JOURNAL   Nature 354 (6350), 233-235 (1991)
   PUBMED   1683685
REFERENCE   7  (bases 1 to 7252)
  AUTHORS   Kudo,S. and Fukuda,M.
  TITLE     A short, novel promoter sequence confers the expression of human
            leukosialin, a major sialoglycoprotein on leukocytes
  JOURNAL   J Biol Chem 266 (13), 8483-8489 (1991)
   PUBMED   1827122
REFERENCE   8  (bases 1 to 7252)
  AUTHORS   Park,J.K., Rosenstein,Y.J., Remold-O'Donnell,E., Bierer,B.E.,
            Rosen,F.S. and Burakoff,S.J.
  TITLE     Enhancement of T-cell activation by the CD43 molecule whose
            expression is defective in Wiskott-Aldrich syndrome
  JOURNAL   Nature 350 (6320), 706-709 (1991)
   PUBMED   2023632
REFERENCE   9  (bases 1 to 7252)
  AUTHORS   Shelley,C.S., Remold-O'Donnell,E., Rosen,F.S. and Whitehead,A.S.
  TITLE     Structure of the human sialophorin (CD43) gene. Identification of
            features atypical of genes encoding integral membrane proteins
  JOURNAL   Biochem J 270 (3), 569-576 (1990)
   PUBMED   2241892
REFERENCE   10 (bases 1 to 7252)
  AUTHORS   Shelley,C.S., Remold-O'Donnell,E., Davis,A.E. 3rd, Bruns,G.A.,
            Rosen,F.S., Carroll,M.C. and Whitehead,A.S.
  TITLE     Molecular characterization of sialophorin (CD43), the lymphocyte
            surface sialoglycoprotein defective in Wiskott-Aldrich syndrome
  JOURNAL   Proc Natl Acad Sci U S A 86 (8), 2819-2823 (1989)
   PUBMED   2784859
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1989 Jun;86(12):4689]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK292626.1, AC009086.6 and
            BQ774811.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003123.5.
            
            Summary: This gene encodes a highly sialylated glycoprotein that
            functions in antigen-specific activation of T cells, and is found
            on the surface of thymocytes, T lymphocytes, monocytes,
            granulocytes, and some B lymphocytes. It contains a mucin-like
            extracellular domain, a transmembrane region and a carboxy-terminal
            intracellular region. The extracellular domain has a high
            proportion of serine and threonine residues, allowing extensive
            O-glycosylation, and has one potential N-glycosylation site, while
            the carboxy-terminal region has potential phosphorylation sites
            that may mediate transduction of activation signals. Different
            glycoforms of this protein have been described. In stimulated
            immune cells, proteolytic cleavage of the extracellular domain
            occurs in some cell types, releasing a soluble extracellular
            fragment. Defects in expression of this gene are associated with
            Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017].
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292626.1, SRR1163657.370958.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000652691.1/ ENSP00000498852.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1444              AK292626.1         1-1444
            1445-6853           AC009086.6         175193-180601
            6854-7252           BQ774811.1         13-411              c
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p11.2"
     gene            1..7252
                     /gene="SPN"
                     /gene_synonym="CD43; GALGP; GPL115; LEU-22; LSN"
                     /note="sialophorin"
                     /db_xref="GeneID:6693"
                     /db_xref="HGNC:HGNC:11249"
                     /db_xref="MIM:182160"
     exon            1..70
                     /gene="SPN"
                     /gene_synonym="CD43; GALGP; GPL115; LEU-22; LSN"
                     /inference="alignment:Splign:2.1.0"
     exon            71..7252
                     /gene="SPN"
                     /gene_synonym="CD43; GALGP; GPL115; LEU-22; LSN"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..1307
                     /gene="SPN"
                     /gene_synonym="CD43; GALGP; GPL115; LEU-22; LSN"
                     /note="sialophorin (gpL115, leukosialin, CD43);
                     galactoglycoprotein; leukocyte sialoglycoprotein"
                     /codon_start=1
                     /product="leukosialin precursor"
                     /protein_id="NP_003114.1"
                     /db_xref="CCDS:CCDS10650.1"
                     /db_xref="GeneID:6693"
                     /db_xref="HGNC:HGNC:11249"
                     /db_xref="MIM:182160"
                     /translation="MATLLLLLGVLVVSPDALGSTTAVQTPTSGEPLVSTSEPLSSKM
                     YTTSITSDPKADSTGDQTSALPPSTSINEGSPLWTSIGASTGSPLPEPTTYQEVSIKM
                     SSVPQETPHATSHPAVPITANSLGSHTVTGGTITTNSPETSSRTSGAPVTTAASSLET
                     SRGTSGPPLTMATVSLETSKGTSGPPVTMATDSLETSTGTTGPPVTMTTGSLEPSSGA
                     SGPQVSSVKLSTMMSPTTSTNASTVPFRNPDENSRGMLPVAVLVALLAVIVLVALLLL
                     WRRRQKRRTGALVLSRGGKRNGVVDAWAGPAQVPEEGAVTVTVGGSGGDKGSGFPDGE
                     GSSRRPTLTTFFGRRKSRQGSLAMEELKSGSGPSLKGEEEPLVASEDGAVDAPAPDEP
                     EGGDGAAP"
     regulatory      7219..7224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPN"
                     /gene_synonym="CD43; GALGP; GPL115; LEU-22; LSN"
                     /note="hexamer: AATAAA"
     polyA_site      7252
                     /gene="SPN"
                     /gene_synonym="CD43; GALGP; GPL115; LEU-22; LSN"
                     /note="major polyA site"
ORIGIN      
        1 agtcttgccc cagcctcggg aggtggtgga gtgacctggc cccagtgctg cgtccttatc
       61 agccgagccg gtcccagctc ttgctcctgc ctgtttgcct ggaaatggcc acgcttctcc
      121 ttctccttgg ggtgctggtg gtaagcccag acgctctggg gagcacaaca gcagtgcaga
      181 cacccacctc cggagagcct ttggtctcta ctagcgagcc cctgagctca aagatgtaca
      241 ccacttcaat aacaagtgac cctaaggccg acagcactgg ggaccagacc tcagccctac
      301 ctccctcaac ttccatcaat gagggatccc ctctttggac ttccattggt gccagcactg
      361 gttccccttt acctgagcca acaacctacc aggaagtttc catcaagatg tcatcagtgc
      421 cccaggaaac ccctcatgca accagtcatc ctgctgttcc cataacagca aactctctag
      481 gatcccacac cgtgacaggt ggaaccataa caacgaactc tccagaaacc tccagtagga
      541 ccagtggagc ccctgttacc acggcagcta gctctctgga gacctccaga ggcacctctg
      601 gaccccctct taccatggca actgtctctc tggagacttc caaaggcacc tctggacccc
      661 ctgttaccat ggcaactgac tctctggaga cctccactgg gaccactgga ccccctgtta
      721 ccatgacaac tggctctctg gagccctcca gcggggccag tggaccccag gtctctagcg
      781 taaaactatc tacaatgatg tctccaacga cctccaccaa cgcaagcact gtgcccttcc
      841 ggaacccaga tgagaactca cgaggcatgc tgccagtggc tgtgcttgtg gccctgctgg
      901 cggtcatagt cctcgtggct ctgctcctgc tgtggcgccg gcggcagaag cggcggactg
      961 gggccctcgt gctgagcaga ggcggcaagc gtaacggggt ggtggacgcc tgggctgggc
     1021 cagcccaggt ccctgaggag ggggccgtga cagtgaccgt gggagggtcc gggggcgaca
     1081 agggctctgg gttccccgat ggggaggggt ctagccgtcg gcccacgctc accactttct
     1141 ttggcagacg gaagtctcgc cagggctccc tggcgatgga ggagctgaag tctgggtcag
     1201 gccccagcct caaaggggag gaggagccac tggtggccag tgaggatggg gctgtggacg
     1261 ccccagctcc tgatgagccc gaagggggag acggggctgc cccttaagtg tcggtgaata
     1321 gtgaggctgg aggccggaat ctcagccagc ctccagcacc ttccctctca ccatcccact
     1381 gccccctcgc tcccatgttt ccacccggca ccctgatcct cacccgaatc tccttttttt
     1441 ttttcttttg agacagagtt tcgctttgtc gcccaggctg gagtgcaatg cacgatctca
     1501 gttcactgca acctctgcct cctaagttca ggcgattctc ctgcctcagc ttcccgagta
     1561 actgagatta caggcaccca ccaccatgcc cagctgcttt tttgtatttt tggtagagat
     1621 ggggtttcac catgttggct aggctggtct caaactcctg acctcaggtg atctacctgc
     1681 ctcagcctcc caaagtgctg agattacaga catgagcctc cgcgccttgc ctcctcaccc
     1741 acctcttcac tctgaatcct catgaggctt ctcagccctg gatttcctgc tgccatcctc
     1801 acccagcacc cacaactagc gcctgggcag ggcagggctg gcacctctca acgtctgtgg
     1861 actgaatgaa taaaccctcc tcatccaccc ctatttatct ccatcaccat ttccccctct
     1921 ttcttgttcc tggaaacggc tgctgagtct ccatcggcca aacttatctg ccctgtgatt
     1981 tctttgacaa ttctcctttt cccccagaac ccaccctggg ttgaccagag tctgggaaga
     2041 aggacaagag aacccggcaa actccctcct aggattaact ttgtaaagca cccttgccct
     2101 gtagctgcaa gggctgtgga acctgggcag cccgcaacca cctttagctc tgggcccccc
     2161 aggccagcct ggagcatggc tgggtggggc caccagccca tgctctcagg cgggcctgtg
     2221 atctttccca gggcacatgg actgtaggct ggccctggcc cacaccacca cactctcccc
     2281 agccatggac agaggcagcc agaggcctca cggtttctcc tccgagtttc tggctgggtg
     2341 tagttctcag aaaccccagt gcctgcgtgt gtccactcgt gggtgtggtt tgtgtgcaag
     2401 agctgaggat ttggcgatgc ttgggagggg tagttgtggg tacagacggt gtgggggtgg
     2461 gaagtggtgc agagactgaa gagggtcaac ctgggcatgg gggacacagg gactgctgag
     2521 aacgtgcgtg tcatctttgc tctgatgggg tggacatagc agaaaatcta actctgtctg
     2581 tagccccata cagaatgcca gggtgagcac agtggctggt gcctttaatc ccagcacttt
     2641 ggaaagttga ggcaggagga tcgcttgagc ccaggagttc gagtctgaag tgagctgtga
     2701 ttgcaccact gcacttcagc ctgggcaaca gagtgagccc ctgtctcaaa aaagaaaaga
     2761 aaaagaaagc caggcttcat ggaaagatcg tatgtgtgac ccaaatatga gttcttcagc
     2821 tcagccatgg taatcccttc cttgaagtct ccatttctgc agtacacatg catgtgcgct
     2881 ctctctctct ctctctctct cacacacaca cacacacaca cacacacacg cgcgcgcgcg
     2941 cgcgctctcc tgcgaacaga ggcaggggga gaggggtttg ccctggtctc ggggactggt
     3001 ctggctggcg cttccccact gcacgtttcc aggtttagtt tgtctgtgtc tcctcttcca
     3061 tcccaggggc tgagcccctt ccatcctcca agaggaacca gtgagagtga gtgaaggagg
     3121 ggcctggagc cagggacttc ccctgtgggg cctgggtgga gaggggagaa ctcaatggtg
     3181 ctgcctttga gaccagccca ggctacagcc caggagcaca catgggccag ggcagttggt
     3241 atttcccgag gacaaagagg aaattttcaa agaggaagtt gttgagttag agcttgcggt
     3301 ggctgagagc agacaggttg acctgcaaaa aaagacaggg gaggcatgtg agtgtgacag
     3361 ccctgctctg tggcctgggc aggagatggg ggaaagggtc aggtggggga tgggctcgtg
     3421 cagtgggaga ggagacggag ggagggagcg ggaaggggct tgcttagtgg gtgggaagag
     3481 ctgagctcgg atggaaccag cttctaccag ccaggctggg cacccactgg gctgcatctg
     3541 gtggcctttt ctgattgcta tttggactca ctgcagctgc agaatgacag aggccatgtc
     3601 caaaatccct tagagacact gttgtcttag agttgttaaa ataagagccc ccatatcagg
     3661 tttagaaaat actgtcaccg aacgaacgtc gctgtcctca gctccacctc cctttccttt
     3721 gacagatatg gttgttttct aagccaggac tggttttagt caggtcctgg gcgaatcctg
     3781 aaaaaaagag gtagtacggg taaggaaggc acccaacagg gctttcacaa tccagaaaat
     3841 atcaaaatat aagtgttaaa agagaggcac aggccgggtg cggtggctca cgcctgtaat
     3901 ctcagcactt tgggaggcca aggtgggcag atcatgaggt caggagtttg agaccagcct
     3961 ggccaatatg atgaaacccc gtttctacta aaaatacaaa agttagccag gcatggtggt
     4021 gtgctcctgt aatcccagct acttaggagg ctgaggccag agaattgctt gaaccctgga
     4081 gtcagaggtt gcagtgagcc gggatcatgc cactgtactc caggctgggt gacaaagtga
     4141 gactgtctca aaaaataaaa ataaataaaa taaataaaag agaggcacaa acagtgttat
     4201 gaatgcacca aggaaaatgg tgcattcata actctcaggt gaagcctacc aagccatgcg
     4261 tgtgtgcaca tatgtgtgta cgtgtgcatg tgcgtgcgtg catgtgcgtg cgtgcatgtg
     4321 cctgtgtgtg tatgtgtgca catgtgtgtg cgcatgtgtg tgtgtgcgcg catgtgtgtg
     4381 tgcatgcatg ttctcccatg catgtgtact gtggcaaggg agactttgag gaagagattc
     4441 cagtggctga gcagaagggc tcgcattgcc ctggcgaaag gttggaaggc ttcacctgag
     4501 agtgtgtcgt ggcctttgtc atatccactg cttgattcct ttctttaaaa attattttta
     4561 ttgttttcta catatgagaa ccaccacacc tggctaattt ttgtattttt tgtagagatg
     4621 gggtttcacc atgttgtccc ggctggtctc aaactcccgg gcacaagaga tccacctgcc
     4681 tcagcctccc aaaatgctgg gactataggc atgagccact gcacccagcc actgcttcat
     4741 tcctggtggc tgctgtgcct ggcatgttgc agatcctcca tgaatatgca tttgaatgaa
     4801 tgaatgaatg aatgaatgaa tgaatggaga tgacgcctca gagattcttt cttttgagat
     4861 gaggtctcat tctgtcaccc agactagagg gcagtggtgc aatcacagct caccacagcc
     4921 tcaacctcct gggcctccca agtagctgcg atcacaggtg tgcaccaaca tgcccagcta
     4981 attttttttt ttaattttta atttgtacag acagggtctt gctgtgttgc ccaggctggt
     5041 ctcaaactcc tgggctcaag tggtcctccc acctaagctt ccccaaatac tgggattata
     5101 ggtgtgagcc actgtgccca ggcttgcctc agatatttga aggctgggaa ggattttgca
     5161 aagctgggaa aaggaaaagg cattcccagc agaggggata gcaggtggaa atacataatt
     5221 aaaaaaaaaa aacgtggagc agatccagcg cagtggctca tgcctgtaat cccagcactt
     5281 tgggaggcag agcagggagg attgcttgag tctaggagtt caagaccagc ctgggtaaca
     5341 tagaaagacc ctgtctctac aaaaacacaa aaaattagcc aggcgtggtg gtgcatgcct
     5401 gtagtaccag ctacttgaga agctgaggca ggaggactgc ttgaagccag gagtttgaga
     5461 ccagcctggg caacatagtg agaccccgtg tctacaaaaa gtaaacattt atatatatat
     5521 tttttaaagt ggagcagttc aatatagagt cttttttgaa caaacgtgaa atagatgtct
     5581 tttttttttt tttgagatgg agttttcact cttgttaccc aggctggagt gcaatggcgt
     5641 gatcttggct caccagaacc tccgcctcct gggttcaaac aattctcctg cctcagcctc
     5701 ccaggtagct gggattacag gcatgcacca ccaaacccgg ataattttgt atttttagta
     5761 gagatggggt ttcaccatgt tggtcaagct ggtcttgaac tcccgacctc tgctgatccg
     5821 tatgcctcgg cctcccaaag tgctgggatt acatgcgtga gccaccgtgc ccgacaatag
     5881 atgtctttta attttctgga ggaaaaagca aagcaaaaga agcagtggat attttaagac
     5941 taaaaaggaa aacaaaaaaa ggagatagag caggccagac gtggtggctc aacgtctgta
     6001 atcccagcac tttgggaggc cgaggcaggt ggatcacctg aggtcaggag ttcaagacca
     6061 gcctgaccaa catggtgaaa ccctgtttca aaatacaaaa aattagctgg gcgtggtggc
     6121 gggcacctgt aatcccagct acttgggagg ctgaggcagg agaatccctt gaacccagga
     6181 ggtggaggtt gcagtgagcc gagatcacgc cattgcactc cagcctgggc gacaagtaaa
     6241 aaactccatc tcaaaaaaaa aaaaggagat agagcaagga acagtaagaa aatagttggg
     6301 tgcagtggct atgcggtggc actataggag gctgaggcgg gcagatcacc tgaggtcagg
     6361 agttggagac cagcctgggc aacatagacc cctatctcta caaaaaattt gaaatatgaa
     6421 aaattagcca ggtgtagtgg tgtgcgcctg tggtaccagc tactcaagag gcgaaggcag
     6481 ggaagattgc ttgagcccag gagtttgagg ctatagtgag ctgtgatcat gccactgcac
     6541 tccagcctgg gcaatagtgt gagactctgt ctcaaaagaa agaacatggc caggcgtggt
     6601 ggctcacacc tgtaatccca gcactttggg aggccgaggc agtcagatca tgaggccagt
     6661 ttgaaaccag cctggccaac atggtgaaac cctgtctcta ctaaaaatac aaaaattagc
     6721 caggcgtggt ggcatgcccc cgtaatccca gctacttggg ggtctgaggc agaagaattg
     6781 cttgaaaccg ggaggcagag gttgcagtga gccgagatcg tgtcattgca ctctagtctg
     6841 ggcgacagag caagactccg tcttggaaaa aaatttaaaa aaagaaaata taaaatatgg
     6901 tataagatta aatatattag ttatgaaaat aattgtaaat ggcttattga acttatagtc
     6961 agtaaaaatc atgtttttga aaattatgta atgactttgg aaaataccag caatataatg
     7021 ttaagtgggg aaaaaagcaa gttactcctc tgtaatacat atgattttag ttttgtaata
     7081 aaaattccca accatatatg cacttatagg gaaacaaagg acccatcgca aatgttttcc
     7141 atgctgatct ccaaagtggt gagtttatgt gtgattttta ttttgtttat gctcttctgt
     7201 attttccgaa tttcatacaa taaatatctg ttacttttac aatatgaaaa aa
//
