LOCUS       NM_001128626            5638 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens spire type actin nucleation factor 1 (SPIRE1),
            transcript variant 1, mRNA.
ACCESSION   NM_001128626
VERSION     NM_001128626.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Li,J., Xiong,N., West,K.L., Leung,M., Ching,Y.P., Huang,J.,
            Yuan,J., Yu,C.H., Leung,J. and Huen,M.
  TITLE     Nuclear F-actin assembly on damaged chromatin is regulated by
            DYRK1A and Spir1 phosphorylation
  JOURNAL   Nucleic Acids Res 52 (15), 8897-8912 (2024)
   PUBMED   38966995
  REMARK    GeneRIF: Nuclear F-actin assembly on damaged chromatin is regulated
            by DYRK1A and Spir1 phosphorylation.
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Scalzitti,S., Mariani,D., Setti,A., Colantoni,A., Lisi,M.,
            Bozzoni,I. and Martone,J.
  TITLE     Lnc-SMaRT Translational Regulation of Spire1, A New Player in
            Muscle Differentiation
  JOURNAL   J Mol Biol 434 (2), 167384 (2022)
   PUBMED   34863993
  REMARK    GeneRIF: Lnc-SMaRT Translational Regulation of Spire1, A New Player
            in Muscle Differentiation.
REFERENCE   3  (bases 1 to 5638)
  AUTHORS   Holthenrich,A., Terglane,J., Nass,J., Mietkowska,M., Kerkhoff,E.
            and Gerke,V.
  TITLE     Spire1 and Myosin Vc promote Ca2+-evoked externalization of von
            Willebrand factor in endothelial cells
  JOURNAL   Cell Mol Life Sci 79 (2), 96 (2022)
   PUBMED   35084586
  REMARK    GeneRIF: Spire1 and Myosin Vc promote Ca(2+)-evoked externalization
            of von Willebrand factor in endothelial cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5638)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 5638)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 5638)
  AUTHORS   Zeth,K., Pechlivanis,M., Samol,A., Pleiser,S., Vonrhein,C. and
            Kerkhoff,E.
  TITLE     Molecular basis of actin nucleation factor cooperativity: crystal
            structure of the Spir-1 kinase non-catalytic C-lobe domain
            (KIND)*formin-2 formin SPIR interaction motif (FSI) complex
  JOURNAL   J Biol Chem 286 (35), 30732-30739 (2011)
   PUBMED   21705804
  REMARK    GeneRIF: analysis of the molecular basis of the Spir1/formin-2
            interaction
REFERENCE   7  (bases 1 to 5638)
  AUTHORS   Pfender,S., Kuznetsov,V., Pleiser,S., Kerkhoff,E. and Schuh,M.
  TITLE     Spire-type actin nucleators cooperate with Formin-2 to drive
            asymmetric oocyte division
  JOURNAL   Curr Biol 21 (11), 955-960 (2011)
   PUBMED   21620703
REFERENCE   8  (bases 1 to 5638)
  AUTHORS   Pechlivanis,M., Samol,A. and Kerkhoff,E.
  TITLE     Identification of a short Spir interaction sequence at the
            C-terminal end of formin subgroup proteins
  JOURNAL   J Biol Chem 284 (37), 25324-25333 (2009)
   PUBMED   19605360
  REMARK    GeneRIF: both mammalian Spir proteins, Spir-1 and Spir-2, interact
            with mammalian Fmn subgroup proteins formin-1 and formin-2
REFERENCE   9  (bases 1 to 5638)
  AUTHORS   Bosch,M., Le,K.H., Bugyi,B., Correia,J.J., Renault,L. and
            Carlier,M.F.
  TITLE     Analysis of the function of Spire in actin assembly and its synergy
            with formin and profilin
  JOURNAL   Mol Cell 28 (4), 555-568 (2007)
   PUBMED   18042452
  REMARK    GeneRIF: The multifunctional character of the WH2 domains allows
            Spire to sequester four G-actin subunits binding cooperatively in a
            tight SA(4) complex and to nucleate, sever, and cap filaments at
            their barbed ends.
REFERENCE   10 (bases 1 to 5638)
  AUTHORS   Kerkhoff,E., Simpson,J.C., Leberfinger,C.B., Otto,I.M., Doerks,T.,
            Bork,P., Rapp,U.R., Raabe,T. and Pepperkok,R.
  TITLE     The Spir actin organizers are involved in vesicle transport
            processes
  JOURNAL   Curr Biol 11 (24), 1963-1968 (2001)
   PUBMED   11747823
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP005482.4, AB032961.2 and AP001029.5.
            
            On Jun 2, 2019 this sequence version replaced NM_001128626.1.
            
            Summary: Spire proteins, such as SPIRE1, are highly conserved
            between species. They belong to the family of Wiskott-Aldrich
            homology region-2 (WH2) proteins, which are involved in actin
            organization (Kerkhoff et al., 2001 [PubMed 11747823]).[supplied by
            OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB032961.2, SRR1803616.97941.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409402.9/ ENSP00000387266.3
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-194               AP005482.4         41965-42158         c
            195-2848            AB032961.2         53-2706
            2849-5638           AP001029.5         89650-92439         c
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.21"
     gene            1..5638
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /note="spire type actin nucleation factor 1"
                     /db_xref="GeneID:56907"
                     /db_xref="HGNC:HGNC:30622"
                     /db_xref="MIM:609216"
     exon            1..578
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             242..2512
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /note="isoform a is encoded by transcript variant 1;
                     protein spire homolog 1; spire actin nucleation factor 1;
                     spire family actin nucleation factor 1"
                     /codon_start=1
                     /product="protein spire homolog 1 isoform a"
                     /protein_id="NP_001122098.1"
                     /db_xref="CCDS:CCDS45829.1"
                     /db_xref="GeneID:56907"
                     /db_xref="HGNC:HGNC:30622"
                     /db_xref="MIM:609216"
                     /translation="MAQAAGPAGGGEPRTEAVGGEGPREPGAAGGAAGGSRDALSLEE
                     ILRLYNQPINEEQAWAVCYQCCGSLRAAARRRQPRHRVRSAAQIRVWRDGAVTLAPAA
                     DDAGEPPPVAGKLGYSQCMETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDHM
                     ANTVEADGSNDEGYEAAEEGLGDEDEKRKISAIRSYRDVMKLCAAHLPTESDAPNHYQ
                     AVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESSTDLEELKNADWARFWVQV
                     MRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRCKRYTLRKVMVNGDIPPRLK
                     KSAHEIILDFIRSRPPLNPVSARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIR
                     RSRLAMRPLSMSYSFDLSDVTTPESTKNLVESSMVNGGLTSQTKENGLSTSQQVPAQR
                     KKLLRAPTLAELDSSESEEETLHKSTSSSSVSPSFPEEPVLEAVSTRKKPPKFLPISS
                     TPQPERRQPPQRRHSIEKETPTNVRQFLPPSRQSSRSLEEFCYPVECLALTVEEVMHI
                     RQVLVKAELEKYQQYKDIYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCK
                     KMRLPSKPYSTLPIFSLGPSALQRGESSMRSEKPSTAHHRPLRSIARFSSKSKSMDKS
                     DEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSS
                     PGPSEYCPSERTISEI"
     exon            579..613
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            614..844
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            845..970
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1048
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1213
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1300
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1430
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1472
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1645
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1736
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1737..1879
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2017
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2088
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2116
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2253
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..5638
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5617..5622
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /note="hexamer: AATAAA"
     polyA_site      5638
                     /gene="SPIRE1"
                     /gene_synonym="Spir-1"
                     /note="major polyA site"
ORIGIN      
        1 ggacgccatc gcgtcgctac cggccggcgg ctgtctgacc gggactgcag ccgtgtcctc
       61 gccgccctcc tcttgcccac gcctcccggg cactcgcctg gtcccggcgg cggcgcgcgc
      121 ggccgcgttg ggcggcggcg gttccgggga tggtgaggcg gcggcgcgcg acgacgatgc
      181 tccggagctg aggagacgcc ggcacacggc gcgactgccg agcgcagcgc ccacccgcgg
      241 gatggctcag gcggctggcc cggcgggcgg cggggagccg cggactgagg cagtgggcgg
      301 cgaggggccg cgggagcccg gggcagccgg cggcgcggcc gggggctccc gggacgcgct
      361 gagcctggag gagatcctgc ggctgtacaa ccagcccatc aacgaggagc aggcgtgggc
      421 cgtgtgctac cagtgctgcg gttccctgcg cgccgccgcc cgccgccgcc agccccgcca
      481 ccgtgtgcgc tcggccgcgc agatccgcgt ctggagggac ggcgccgtca ccctggcgcc
      541 cgcggccgac gacgcgggag agccgccccc agttgcgggt aaattgggat attcacaatg
      601 tatggaaaca gaggtcattg aatctttggg aattattatt tataaagcac tggactatgg
      661 tttgaaggag aatgaagaaa gggaattaag ccctccccta gagcagctta tcgatcacat
      721 ggccaacacg gtggaagctg acggtagcaa tgatgagggc tatgaggctg cagaagaagg
      781 cctgggagat gaagatgaaa agagaaaaat ctcagctatt cggtcatata gagatgtcat
      841 gaagttgtgt gctgctcatc tccctactga atcagatgca ccaaatcatt atcaggcagt
      901 atgtcgtgca ctgtttgcag aaacaatgga gctccataca tttctgacca aaattaagag
      961 tgcgaaagag aatcttaaga agattcaaga aatggaaaag agcgatgaat ctagcacaga
     1021 cttggaagag ctgaaaaacg ctgactgggc acgattctgg gtacaggtga tgagggattt
     1081 gaggaatggg gtaaaactta agaaggtcca agagcggcag tacaaccctt tgcccattga
     1141 atatcagctc accccttatg agatgttaat ggatgacatt cgctgcaaaa gatacacctt
     1201 gcgaaaagtg atggtgaatg gtgatattcc ccctcggtta aaaaagagtg ctcatgaaat
     1261 catcctcgac ttcatcagat ccagacctcc tttaaatcca gtctcagcca gaaaactgaa
     1321 accaactcca ccacggccac ggagcctcca tgaaagaata ttagaagaaa ttaaagcaga
     1381 aagaaagctg cggcctgtat caccagagga gattagacgt agcagattag caatgcggcc
     1441 acttagcatg tcttacagtt ttgacttgtc agatgtgact acccctgaat ctacaaagaa
     1501 tcttgtggag tcatctatgg tgaatggagg tttgacatca caaacaaaag aaaacgggtt
     1561 aagtacctca cagcaggtgc ctgcacagcg gaagaagctc ctcagagccc caactctggc
     1621 cgaactggac agctctgagt ctgaggaaga aacgctgcac aagtcgacca gcagcagcag
     1681 cgtgtctccc tctttccctg aagagccagt cctggaggcc gtgtccacaa ggaagaagcc
     1741 tccaaaattc ctgcccatat catcaacacc ccagccagag agacggcagc caccccagag
     1801 acgacattcc attgaaaagg aaacgcctac taacgtgagg cagttcctgc cgccttccag
     1861 gcagagttcc cgctctcttg aggaattctg ctacccagtg gaatgcctcg ctcttactgt
     1921 ggaagaagtg atgcatattc gccaggtcct ggtgaaggca gagctggaaa aataccaaca
     1981 gtataaagac atctacaccg ccttgaaaaa aggaaagctc tgcttttgtt gccgaaccag
     2041 gaggttttcc ttcttcactt ggtcttatac ctgtcagttc tgtaagaggc cggtgtgctc
     2101 acagtgttgc aaaaagatgc ggctgccctc caaaccatac tccactcttc ctatcttttc
     2161 attgggacct tctgctctgc aaagagggga aagtagtatg aggtcagaaa aaccctccac
     2221 tgcccatcat cggccacttc ggagcattgc caggttctcc tcaaaatcta agtctatgga
     2281 caaatcagat gaagaactcc agtttcccaa agagttgatg gaggactgga gcaccatgga
     2341 ggtgtgtgtg gactgcaaga agttcatttc ggaaatcatc agttcaagcc ggcgcagtct
     2401 ggtgttggcc aacaaaaggg cccgattgaa aaggaaaacg cagtctttct acatgtcctc
     2461 gccaggcccc tcggagtact gcccttcaga gaggacgatc agtgagatct gagcctcgtg
     2521 cctttcagct gcttttgtgc tacgagtcag cgtccgtgcg cgaggacact gagccgggct
     2581 ggctctcctt tctgtggttt tatttaatgg gcttgaattt gcattagatc agatttttgc
     2641 cgcatcacat tgttccacag actgaatgct gtgttcgtat cgattgatga aacgtgacag
     2701 gtccgccaat tgctcgtttg cactgagaga ggacaacagt ttgaaactta cttttgtgtg
     2761 tgtgtggctt tggaagccag tagctacttc cttagttcag ttctttactg ttcctcgaat
     2821 aatctcctga ctaaggcaaa aaaaaaaagc ttctcctacg agaatcagtc taacagagat
     2881 gccgatgtca gcacagccct aagcagtaag tcatattggc atttccacgt gactgtgttt
     2941 ctatcccgtg tacagagaga tccagagccc tacactccac gacctggggg ctcacagcac
     3001 agaacctaga agcacctgct gacactcttc aactgatttt taaatgttgt tgcttggaga
     3061 taaaaattac ataagggact ttttgcctgc attctagtgc aaaacatctg aagagctgta
     3121 cacccacaag ggtgactatt tcccctgagt ggccgtgttg tcccagtgcc ctggttcagt
     3181 gtctcctgag tggatgacag gtcttcattc tctatcttga atgtattatg gttactaata
     3241 gttttataat ggaggtctaa gaattaaagt tgtgtgggag tttcaggaca aaggaaggct
     3301 aaaagtttgt caagacgttg agcgtatttt ggttacctat gagaagggtt gtgacagtgt
     3361 acagtggcag ctgttggcca cgctgcagaa atgagctgga gctcatgggt tttcagctac
     3421 atttttcata actttgtagt acatccatct tgagtaaatt aagccacaat ttggtaccta
     3481 gggtctcaaa ctaaaattta tttttataaa tgaattttaa aagaaaaaat atctacttct
     3541 tttaaagtta gaagaaaatt aacctgctga caggcaacat ttttggggtg ctttctgcac
     3601 tagttttcct tgtaaatgat ttgagtgagt aggtttggtt tctgacgaaa gtagactgga
     3661 gggtagcatt gtatgcctca aatgtctcag tgtgtttggc tcatacgtgg gctatactgt
     3721 attattttgg tatgcttaca aatgactaac caatcaaatt gtcattaatg tttggaaaat
     3781 ctgttaatgc acatgcacaa taatttcctg aaagccatag gacatgtctg tagtcagcac
     3841 cacgatagca ccgtttcatg aaaggcatgg cggctgcatt tcataccaca tcaaaataca
     3901 gtaacatttc tatactaaat taacagtaat acctcaaaac tgctccggta gtagttttta
     3961 atggattgaa atttacagtt tagtaaaagg cttaaaatta cttatactta tgaaataaac
     4021 tttaccagtt gactaaaata atgcatgtta acagttggtc tgtatttgca tgtaaaagtg
     4081 ggccaccaga gaacccttat tgattactta agtgtttaca ttattttaaa gactcctgtt
     4141 taagagcttt cagaattgta ctgggtgaat ctcatttata aaacttccta agagactatc
     4201 tgaactctat actccagaca gttaggtggg agtataaatc tacccctttt gatgacccca
     4261 ggcttgagtt tttaaaatga ctacccagaa gggcacaagg gggaaggaaa tggtatttgt
     4321 atatgtatat aaatatgcac ctaggagaat gtgcttttta aaataatgac tactgttttt
     4381 attaaaacat aagaaactac acccccaaaa taagactttc attcacattc acaaagcaaa
     4441 catctagtac atgtctttca cttcacttta tgatagtgta ttggatgatt tgggcattac
     4501 gatcacctct taccacagca cagaacatac attcttcaac agcattaacg gagtttgcca
     4561 agtgcattaa agaggtcacg tggagggtac gttcatatga aacaatctgc agaaagtggg
     4621 gtaagaaagg gcacatggca cagttaaagt tgtagaaatc aaattactat cattttttgt
     4681 tgccaaaaca aagtcttaca tttaaccccc ctttctacca cccccctcca cacttcacgt
     4741 cagctacata gtttccacag ggtaattcac taagagcttg tggagcttgg ttttaaaatc
     4801 cttagcctgg tctgacttta ggcatagctt ccagttcttc cttccgtgtc ctggtttctt
     4861 gttcagtttt acttctaatc caacaacaaa agaaatgtct ggctggtctc agctagagtc
     4921 tgatgtgtct tagagcatgt gtgcgtatct gaaccatcat ccctgctctc atctcagctc
     4981 cctccaggcc tgagcaccgg ttccttttgt cccatacgtc atgaagtcac actattggga
     5041 aacctgtgct tccctctcca tggcttaact ccctgtcagt gtcggagtgt ataagaatgc
     5101 ttgtaaatac tgtaatatat ttattaatat ttgaaaggca ttcattcagt ggacagtggg
     5161 aattaactct cccaaggcaa gtgaaaatga atgattgacg tacgttgatt taacaatctt
     5221 actagatttt aattcttaag gatttcaaat gaaaccagaa ggtggttatg taagaggctt
     5281 aaaatgatct tatgtttaaa gagattctgt tattagcacc atgaactcgt actatgaaat
     5341 ttttaagcct tttatttttc taactatatt actgtaggac tggatattag gtgtcatata
     5401 ggaaacacaa aagtttattg ctgtttgcta aagcaaaata gcagaaaatt ttgtatatgc
     5461 aaaactgttg aaggaccata gagaaatgtg tactactgac ggggctttta ctaggcttcc
     5521 tgcgtgtgta aaagtcgagg tattgctggc attcagggtg acatgatggt actaaatgtt
     5581 ttccattaaa gtcttctatt ttaaaattta gagaaaaata aaatggcttt ccatcaga
//
