LOCUS       NM_003710               2978 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens serine peptidase inhibitor, Kunitz type 1 (SPINT1),
            transcript variant 2, mRNA.
ACCESSION   NM_003710
VERSION     NM_003710.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Lu,D.D., Huang,N., Li,S.A., Fang,J.R., Lai,C.H., Wang,J.K.,
            Chan,K.S., Johnson,M.D. and Lin,C.Y.
  TITLE     HAI-1 is required for the novel role of FGFBP1 in maintenance of
            cell morphology and F-actin rearrangement in human keratinocytes
  JOURNAL   Hum Cell 36 (4), 1403-1415 (2023)
   PUBMED   37076641
  REMARK    GeneRIF: HAI-1 is required for the novel role of FGFBP1 in
            maintenance of cell morphology and F-actin rearrangement in human
            keratinocytes.
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Izumi,A., Yamamoto,K., Kawaguchi,M., Yamashita,F., Fukushima,T.,
            Kiwaki,T., Tanaka,H., Yamashita,Y. and Kataoka,H.
  TITLE     Insufficiency of hepatocyte growth factor activator inhibitor-1
            confers lymphatic invasion of tongue carcinoma cells
  JOURNAL   Cancer Sci 113 (6), 2179-2193 (2022)
   PUBMED   35332604
  REMARK    GeneRIF: Insufficiency of hepatocyte growth factor activator
            inhibitor-1 confers lymphatic invasion of tongue carcinoma cells.
REFERENCE   3  (bases 1 to 2978)
  AUTHORS   Yamashita,F., Kaieda,T., Shimomura,T., Kawaguchi,M., Lin,C.Y.,
            Johnson,M.D., Tanaka,H., Kiwaki,T., Fukushima,T. and Kataoka,H.
  TITLE     Role of the polycystic kidney disease domain in matriptase
            chaperone activity and localization of hepatocyte growth factor
            activator inhibitor-1
  JOURNAL   FEBS J 289 (12), 3422-3439 (2022)
   PUBMED   35020274
  REMARK    GeneRIF: Role of the polycystic kidney disease domain in matriptase
            chaperone activity and localization of hepatocyte growth factor
            activator inhibitor-1.
            Review article
REFERENCE   4  (bases 1 to 2978)
  AUTHORS   Wu,Q., Yin,G., Luo,J., Zhang,Y., Ai,T., Tian,J., Jin,Y., Lei,J. and
            Liu,S.
  TITLE     Comprehensive Analysis of the Expression and Prognostic Value of
            SPINT1/2 in Breast Carcinoma
  JOURNAL   Front Endocrinol (Lausanne) 12, 665666 (2021)
   PUBMED   34381422
  REMARK    GeneRIF: Comprehensive Analysis of the Expression and Prognostic
            Value of SPINT1/2 in Breast Carcinoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2978)
  AUTHORS   Borowicz,S., Principe,D.R., Dorman,M.J., McHenry,A.J., Sondarva,G.,
            Kumar,S., Ananthanarayanan,V., Simms,P.E., Hess,A. and Rana,A.
  TITLE     HAI-1 is an independent predictor of lung cancer mortality and is
            required for M1 macrophage polarization
  JOURNAL   PLoS One 16 (6), e0252197 (2021)
   PUBMED   34185790
  REMARK    GeneRIF: HAI-1 is an independent predictor of lung cancer mortality
            and is required for M1 macrophage polarization.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2978)
  AUTHORS   Shimomura,T., Denda,K., Kawaguchi,T., Matsumoto,K., Miyazawa,K. and
            Kitamura,N.
  TITLE     Multiple sites of proteolytic cleavage to release soluble forms of
            hepatocyte growth factor activator inhibitor type 1 from a
            transmembrane form
  JOURNAL   J Biochem 126 (5), 821-828 (1999)
   PUBMED   10544273
REFERENCE   7  (bases 1 to 2978)
  AUTHORS   Lin,C.Y., Anders,J., Johnson,M. and Dickson,R.B.
  TITLE     Purification and characterization of a complex containing
            matriptase and a Kunitz-type serine protease inhibitor from human
            milk
  JOURNAL   J Biol Chem 274 (26), 18237-18242 (1999)
   PUBMED   10373425
REFERENCE   8  (bases 1 to 2978)
  AUTHORS   Kataoka,H., Suganuma,T., Shimomura,T., Itoh,H., Kitamura,N.,
            Nabeshima,K. and Koono,M.
  TITLE     Distribution of hepatocyte growth factor activator inhibitor type 1
            (HAI-1) in human tissues. Cellular surface localization of HAI-1 in
            simple columnar epithelium and its modulated expression in injured
            and regenerative tissues
  JOURNAL   J Histochem Cytochem 47 (5), 673-682 (1999)
   PUBMED   10219059
REFERENCE   9  (bases 1 to 2978)
  AUTHORS   Yamada,T., Tsujioka,Y., Taguchi,J., Takahashi,M., Tsuboi,Y. and
            Shimomura,T.
  TITLE     White matter astrocytes produce hepatocyte growth factor activator
            inhibitor in human brain tissues
  JOURNAL   Exp Neurol 153 (1), 60-64 (1998)
   PUBMED   9743567
REFERENCE   10 (bases 1 to 2978)
  AUTHORS   Shimomura,T., Denda,K., Kitamura,A., Kawaguchi,T., Kito,M.,
            Kondo,J., Kagaya,S., Qin,L., Takata,H., Miyazawa,K. and Kitamura,N.
  TITLE     Hepatocyte growth factor activator inhibitor, a novel Kunitz-type
            serine protease inhibitor
  JOURNAL   J Biol Chem 272 (10), 6370-6376 (1997)
   PUBMED   9045658
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM763214.1, BC018702.1,
            AC012476.8 and AI684245.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_003710.3.
            
            Summary: The protein encoded by this gene is a member of the Kunitz
            family of serine protease inhibitors. The protein is a potent
            inhibitor specific for HGF activator and is thought to be involved
            in the regulation of the proteolytic activation of HGF in injured
            tissues. Alternative splicing results in multiple variants encoding
            different isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 1,
            resulting in a shorter protein (isoform 2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.1120289.1, BC018702.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000562057.6/ ENSP00000457076.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 BM763214.1         6-13
            9-1430              BC018702.1         1-1422
            1431-1492           AC012476.8         169349-169410
            1493-1540           AC012476.8         169624-169671
            1541-2387           AC012476.8         170118-170964
            2388-2426           AI684245.1         11-49               c
            2427-2978           AC012476.8         171004-171555
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     gene            1..2978
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /note="serine peptidase inhibitor, Kunitz type 1"
                     /db_xref="GeneID:6692"
                     /db_xref="HGNC:HGNC:11246"
                     /db_xref="MIM:605123"
     exon            1..139
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            140..679
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..1746
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; serine protease inhibitor, Kunitz type 1;
                     Kunitz-type protease inhibitor 1; HAI-1; hepatocyte growth
                     factor activator inhibitor type 1"
                     /codon_start=1
                     /product="kunitz-type protease inhibitor 1 isoform 2
                     precursor"
                     /protein_id="NP_003701.1"
                     /db_xref="CCDS:CCDS45231.1"
                     /db_xref="GeneID:6692"
                     /db_xref="HGNC:HGNC:11246"
                     /db_xref="MIM:605123"
                     /translation="MAPARTMARARLAPAGIPAVALWLLCTLGLQGTQAGPPPAPPGL
                     PAGADCLNSFTAGVPGFVLDTNASVSNGATFLESPTVRRGWDCVRACCTTQNCNLALV
                     ELQPDRGEDAIAACFLINCLYEQNFVCKFAPREGFINYLTREVYRSYRQLRTQGFGGS
                     GIPKAWAGIDLKVQPQEPLVLKDVENTDWRLLRGDTDVRVERKDPNQVELWGLKEGTY
                     LFQLTVTSSDHPEDTANVTVTVLSTKQTEDYCLASNKVGRCRGSFPRWYYDPTEQICK
                     SFVYGGCLGNKNNYLREEECILACRGVQGPSMERRHPVCSGTCQPTQFRCSNGCCIDS
                     FLECDDTPNCPDASDEAACEKYTSGFDELQRIHFPSDKGHCVDLPDTGLCKESIPRWY
                     YNPFSEHCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREIPIPSTGSVEM
                     AVAVFLVICIVVVVAILGYCFFKNQRKDFHGHHHHPPPTPASSTVSTTEDTEHLVYNH
                     TTRPL"
     exon            680..807
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            808..946
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1117
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1144
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1270
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1321
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1492
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1540
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..2978
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2408..2413
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /note="hexamer: AATAAA"
     polyA_site      2426
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /note="major polyA site"
     polyA_site      2978
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
ORIGIN      
        1 agtttcacca gaaaccaggg ggagaaggcg gccgagcccc agctctccga gcaccgggtc
       61 ggaagccgcg acccgagccg cgcaggaagc tgggaccgga acctcggcgg acccggcccc
      121 acccaactca cctgcgcagg tcaccagcac cctcggaacc cagaggcccg cgctctgaag
      181 gtgacccccc tggggaggaa ggcgatggcc cctgcgagga cgatggcccg cgcccgcctc
      241 gccccggccg gcatccctgc cgtcgccttg tggcttctgt gcacgctcgg cctccagggc
      301 acccaggccg ggccaccgcc cgcgccccct gggctgcccg cgggagccga ctgcctgaac
      361 agctttaccg ccggggtgcc tggcttcgtg ctggacacca acgcctcggt cagcaacgga
      421 gctaccttcc tggagtcccc caccgtgcgc cggggctggg actgcgtgcg cgcctgctgc
      481 accacccaga actgcaactt ggcgctagtg gagctgcagc ccgaccgcgg ggaggacgcc
      541 atcgccgcct gcttcctcat caactgcctc tacgagcaga acttcgtgtg caagttcgcg
      601 cccagggagg gcttcatcaa ctacctcacg agggaagtgt accgctccta ccgccagctg
      661 cggacccagg gctttggagg gtctgggatc cccaaggcct gggcaggcat agacttgaag
      721 gtacaacccc aggaacccct ggtgctgaag gatgtggaaa acacagattg gcgcctactg
      781 cggggtgaca cggatgtcag ggtagagagg aaagacccaa accaggtgga actgtgggga
      841 ctcaaggaag gcacctacct gttccagctg acagtgacta gctcagacca cccagaggac
      901 acggccaacg tcacagtcac tgtgctgtcc accaagcaga cagaagacta ctgcctcgca
      961 tccaacaagg tgggtcgctg ccggggctct ttcccacgct ggtactatga ccccacggag
     1021 cagatctgca agagtttcgt ttatggaggc tgcttgggca acaagaacaa ctaccttcgg
     1081 gaagaagagt gcattctagc ctgtcggggt gtgcaaggcc cctccatgga aaggcgccat
     1141 ccagtgtgct ctggcacctg tcagcccacc cagttccgct gcagcaatgg ctgctgcatc
     1201 gacagtttcc tggagtgtga cgacaccccc aactgccccg acgcctccga cgaggctgcc
     1261 tgtgaaaaat acacgagtgg ctttgacgag ctccagcgca tccatttccc cagtgacaaa
     1321 gggcactgcg tggacctgcc agacacagga ctctgcaagg agagcatccc gcgctggtac
     1381 tacaacccct tcagcgaaca ctgcgcccgc tttacctatg gtggttgtta cggcaacaag
     1441 aacaactttg aggaagagca gcagtgcctc gagtcttgtc gcggcatctc caagaaggat
     1501 gtgtttggcc tgaggcggga aatccccatt cccagcacag gctctgtgga gatggctgtc
     1561 gcagtgttcc tggtcatctg cattgtggtg gtggtagcca tcttgggtta ctgcttcttc
     1621 aagaaccaga gaaaggactt ccacggacac caccaccacc caccacccac ccctgccagc
     1681 tccactgtct ccactaccga ggacacggag cacctggtct ataaccacac cacgcggccc
     1741 ctctgagcct gggtctcacc ggctctcacc tggccctgct tcctgcttgc caaggcagag
     1801 gcctgggctg ggaaaaactt tggaaccaga ctcttgcctg tttcccaggc ccactgtgcc
     1861 tcagagacca gggctccagc ccctcttgga gaagtctcag ctaagctcac gtcctgagaa
     1921 agctcaaagg tttggaagga gcagaaaacc cttgggccag aagtaccaga ctagatggac
     1981 ctgcctgcat aggagtttgg aggaagttgg agttttgttt cctctgttca aagctgcctg
     2041 tccctacccc atggtgctag gaagaggagt ggggtggtgt cagaccctgg aggccccaac
     2101 cctgtcctcc cgagctcctc ttccatgctg tgcgcccagg gctgggagga aggacttccc
     2161 tgtgtagttt gtgctgtaaa gagttgcttt ttgtttattt aatgctgtgg catgggtgaa
     2221 gaggagggga agaggcctgt ttggcctctc tatcctctct tcctcttccc ccaagattga
     2281 gctctctgcc cttgatcagc cccaccctgg cctagaccag cagacagagc caggagaagc
     2341 tcagctgcat tccgcagccc ccacccccaa ggttctccaa catcacagcc cagcccgccc
     2401 actgggtaat aaaagtggtt tgtggagttt ctggctctgt tctgtgggcc aggagaggga
     2461 ctaggttgct ggggggtgat gggaccgtcc tgttcagctg gttcaggctt cagccaccct
     2521 gttgagcagg ttttcccatc gtctctctcg aaactggcct ggcccctgac tttggagagc
     2581 agtgccatgt ttgaccacca gaggggagga gtgagcgggc cacgggagcc acgagtgtgg
     2641 ggaggctgca gtgctgctgt gtagcatttc ttcctggtgt gagcctctcc acaagcctgt
     2701 tgagtggttg agctctccac aattctgctc atttggaggt tggggttagg ggtgcacagg
     2761 caccttggct cctacctcca tgttagggcc caggtgacta gctgtctccc tcccaacaga
     2821 agccctggct tctgcgctgg agtgcagcac ccccaaccag gctctgagct ccttcccagg
     2881 agcctttagt agagacacgc cactccaacc cacacccctg catttgttca agctacctcc
     2941 cggtgccaaa aaaaaaaaaa aatcatactt tgagctca
//
