LOCUS       NM_001040433             456 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens serine peptidase inhibitor Kazal type 9 (SPINK9),
            mRNA.
ACCESSION   NM_001040433 XM_926728 XM_940086
VERSION     NM_001040433.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 456)
  AUTHORS   Sperrhacke,M., Fischer,J., Wu,Z., Klunder,S., Sedlacek,R.,
            Schroeder,J.M., Meyer-Hoffert,U. and Reiss,K.
  TITLE     SPINK9 stimulates metalloprotease/EGFR-dependent keratinocyte
            migration via purinergic receptor activation
  JOURNAL   J Invest Dermatol 134 (6), 1645-1654 (2014)
   PUBMED   24441102
  REMARK    GeneRIF: SPINK9 has a role in stimulating
            metalloprotease/EGFR-dependent keratinocyte migration by purinergic
            receptor pathway activation
REFERENCE   3  (bases 1 to 456)
  AUTHORS   Brannstrom,K., Ohman,A., von Pawel Rammingen,U., Olofsson,A. and
            Brattsand,M.
  TITLE     Characterization of SPINK9, a KLK5-specific inhibitor expressed in
            palmo-plantar epidermis
  JOURNAL   Biol Chem 393 (5), 369-377 (2012)
   PUBMED   22505519
  REMARK    GeneRIF: the inhibition and binding of different SPINK9 variants
            towards KLK5, KLK7, KLK8 and KLK14
REFERENCE   4  (bases 1 to 456)
  AUTHORS   Reiss,K., Meyer-Hoffert,U., Fischer,J., Sperrhacke,M., Wu,Z.,
            Dimitrieva,O., Krenek,P., Suchanova,S., Buryova,H., Brauer,R. and
            Sedlacek,R.
  TITLE     Expression and regulation of murine SPINK12, a potential orthologue
            of human LEKTI2
  JOURNAL   Exp Dermatol 20 (11), 905-910 (2011)
   PUBMED   21899598
  REMARK    GeneRIF: mSPINK12 shows an inhibitory activity against murine
            keratinocyte-derived trypsin-like proteolytic activity, and thus,
            appears orthologous to human LEKTI2 and may play an role in the
            regulation of epithelial cell functions.
REFERENCE   5  (bases 1 to 456)
  AUTHORS   Brattsand,M., Stefansson,K., Hubiche,T., Nilsson,S.K. and
            Egelrud,T.
  TITLE     SPINK9: a selective, skin-specific Kazal-type serine protease
            inhibitor
  JOURNAL   J Invest Dermatol 129 (7), 1656-1665 (2009)
   PUBMED   19194479
  REMARK    GeneRIF: possible role for SPINK9 in the site-specific epidermal
            differentiation of palms and soles
REFERENCE   6  (bases 1 to 456)
  AUTHORS   Meyer-Hoffert,U., Wu,Z. and Schroder,J.M.
  TITLE     Identification of lympho-epithelial Kazal-type inhibitor 2 in human
            skin as a kallikrein-related peptidase 5-specific protease
            inhibitor
  JOURNAL   PLoS One 4 (2), e4372 (2009)
   PUBMED   19190773
  REMARK    GeneRIF: A new KLK5-inhibiting peptide encoded by a member of the
            Spink family, designated as Spink9 located on chromosome 5p33.1,
            was identified.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY396740.1 and AC091948.3.
            
            On Nov 22, 2018 this sequence version replaced NM_001040433.1.
            
            Summary: The protein encoded by this gene is a Kazal-type serine
            protease inhibitor that appears to specifically target
            kallikrein-related peptidase 5 (KLK5) in the palmo-plantar
            epidermis. KLK5 is an important initiator of skin desquamation, so
            the encoded protease inhibitor may regulate skin differentiation in
            the palms of hands and soles of feet. This cationic protein has
            also been shown to promote keratinocyte migration by activation of
            the epidermal growth factor receptor (EGFR). [provided by RefSeq,
            Dec 2015].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY396740.1, BC137530.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03465418 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377906.2/ ENSP00000367139.1
            RefSeq Select criteria :: based on computational evidence
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-453               AY396740.1         1-453
            454-456             AC091948.3         89660-89662
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q32"
     gene            1..456
                     /gene="SPINK9"
                     /gene_synonym="LEKTI2"
                     /note="serine peptidase inhibitor Kazal type 9"
                     /db_xref="GeneID:643394"
                     /db_xref="HGNC:HGNC:32951"
                     /db_xref="MIM:613511"
     exon            1..110
                     /gene="SPINK9"
                     /gene_synonym="LEKTI2"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..316
                     /gene="SPINK9"
                     /gene_synonym="LEKTI2"
                     /note="serine protease inhibitor Kazal-type 9;
                     lymphoepithelial Kazal-type-related inhibitor 2"
                     /codon_start=1
                     /product="serine protease inhibitor Kazal-type 9
                     precursor"
                     /protein_id="NP_001035523.1"
                     /db_xref="CCDS:CCDS34269.1"
                     /db_xref="GeneID:643394"
                     /db_xref="HGNC:HGNC:32951"
                     /db_xref="MIM:613511"
                     /translation="MRATAIVLLLALTLATMFSIECAKQTKQMVDCSHYKKLPPGQQR
                     FCHHMYDPICGSDGKTYKNDCFFCSKVKKTDGTLKFVHFGKC"
     exon            111..142
                     /gene="SPINK9"
                     /gene_synonym="LEKTI2"
                     /inference="alignment:Splign:2.1.0"
     exon            143..270
                     /gene="SPINK9"
                     /gene_synonym="LEKTI2"
                     /inference="alignment:Splign:2.1.0"
     exon            271..456
                     /gene="SPINK9"
                     /gene_synonym="LEKTI2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      435..440
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPINK9"
                     /gene_synonym="LEKTI2"
                     /note="hexamer: AATAAA"
     polyA_site      456
                     /gene="SPINK9"
                     /gene_synonym="LEKTI2"
                     /note="major polyA site"
ORIGIN      
        1 ggacaccagg tcacttcttt tccctacatc tgactgcctt ggccacttca gtactatgag
       61 agcaacagcc atagtcctac tcttggctct gacacttgca accatgttca gtatagaatg
      121 tgccaaacag acgaaacaga tggttgactg cagtcattat aaaaagttac caccaggaca
      181 acagagattt tgtcatcata tgtatgatcc aatttgtgga tctgatggca aaacttataa
      241 aaatgattgc ttcttctgtt ctaaagttaa gaaaactgac ggcacactta aatttgtaca
      301 ttttggaaaa tgttaaatct atcttgtgag tccaaaatat cttttaatgc atctattcac
      361 aagagtcaca tttctgttcc catattatct atgccacatt gcctactcat caccatatgt
      421 agatttcttt gtagaataaa gcagatataa gggaaa
//
