LOCUS       NM_205841                485 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens serine peptidase inhibitor Kazal type 6 (SPINK6),
            transcript variant 1, mRNA.
ACCESSION   NM_205841
VERSION     NM_205841.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Wang,D., Li,C., Chiu,M.C., Yu,Y., Liu,X., Zhao,X., Huang,J.,
            Cheng,Z., Yuan,S., Poon,V., Cai,J.P., Chu,H., Chan,J.F., To,K.K.,
            Yuen,K.Y. and Zhou,J.
  TITLE     SPINK6 inhibits human airway serine proteases and restricts
            influenza virus activation
  JOURNAL   EMBO Mol Med 14 (1), e14485 (2022)
   PUBMED   34826211
  REMARK    GeneRIF: SPINK6 inhibits human airway serine proteases and
            restricts influenza virus activation.
REFERENCE   2  (bases 1 to 485)
  AUTHORS   Kim,H.J., Shim,J.H., Park,J.H., Shin,H.T., Shim,J.S., Jang,K.T.,
            Park,W.Y., Lee,K.H., Kwon,E.J., Jang,H.S., Yang,H., Lee,J.H.,
            Yang,J.M. and Lee,D.
  TITLE     Single-cell RNA sequencing of human nail unit defines RSPO4
            onychofibroblasts and SPINK6 nail epithelium
  JOURNAL   Commun Biol 4 (1), 692 (2021)
   PUBMED   34099859
  REMARK    GeneRIF: Single-cell RNA sequencing of human nail unit defines
            RSPO4 onychofibroblasts and SPINK6 nail epithelium.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 485)
  AUTHORS   Ge,K., Huang,J., Wang,W., Gu,M., Dai,X., Xu,Y., Wu,H., Li,G.,
            Lu,H., Zhong,J. and Huang,Q.
  TITLE     Serine protease inhibitor kazal-type 6 inhibits tumorigenesis of
            human hepatocellular carcinoma cells via its extracellular action
  JOURNAL   Oncotarget 8 (4), 5965-5975 (2017)
   PUBMED   27999203
  REMARK    GeneRIF: Study showed that the expression of SPINK6 is specifically
            suppressed in liver tumor tissues. The suppression could be even
            detected in the early stage tumors. As SPINK6 is a secretory
            protein, the extracellular protein level may be decreased because
            of tumor development. These results support that SPINK6 is an
            important tumor suppressor in liver.
REFERENCE   4  (bases 1 to 485)
  AUTHORS   Zheng,L.S., Yang,J.P., Cao,Y., Peng,L.X., Sun,R., Xie,P.,
            Wang,M.Y., Meng,D.F., Luo,D.H., Zou,X., Chen,M.Y., Mai,H.Q.,
            Guo,L., Guo,X., Shao,J.Y., Huang,B.J., Zhang,W. and Qian,C.N.
  TITLE     SPINK6 Promotes Metastasis of Nasopharyngeal Carcinoma via Binding
            and Activation of Epithelial Growth Factor Receptor
  JOURNAL   Cancer Res 77 (2), 579-589 (2017)
   PUBMED   27671677
  REMARK    GeneRIF: our results identified a novel EGFR-activating mechanism
            in which SPINK6 has a critical role in promoting nasopharyngeal
            carcinoma metastasis
REFERENCE   5  (bases 1 to 485)
  AUTHORS   Plaza,K., Kalinska,M., Bochenska,O., Meyer-Hoffert,U., Wu,Z.,
            Fischer,J., Falkowski,K., Sasiadek,L., Bielecka,E., Potempa,B.,
            Kozik,A., Potempa,J. and Kantyka,T.
  TITLE     Gingipains of Porphyromonas gingivalis Affect the Stability and
            Function of Serine Protease Inhibitor of Kazal-type 6 (SPINK6), a
            Tissue Inhibitor of Human Kallikreins
  JOURNAL   J Biol Chem 291 (36), 18753-18764 (2016)
   PUBMED   27354280
  REMARK    GeneRIF: a significant fraction of SPINK6-sensitive proteases in
            healthy saliva and confirmed the ability of gingipains to
            inactivate SPINK6 under ex vivo conditions
REFERENCE   6  (bases 1 to 485)
  AUTHORS   Meyer-Hoffert,U., Wu,Z., Kantyka,T., Fischer,J., Latendorf,T.,
            Hansmann,B., Bartels,J., He,Y., Glaser,R. and Schroder,J.M.
  TITLE     Isolation of SPINK6 in human skin: selective inhibitor of
            kallikrein-related peptidases
  JOURNAL   J Biol Chem 285 (42), 32174-32181 (2010)
   PUBMED   20667819
  REMARK    GeneRIF: Data suggest that SPINK6 plays a role in modulating the
            activity of kallikreins in human skin.
REFERENCE   7  (bases 1 to 485)
  AUTHORS   Zhang,Z. and Henzel,W.J.
  TITLE     Signal peptide prediction based on analysis of experimentally
            verified cleavage sites
  JOURNAL   Protein Sci 13 (10), 2819-2824 (2004)
   PUBMED   15340161
REFERENCE   8  (bases 1 to 485)
  AUTHORS   Puente,X.S. and Lopez-Otin,C.
  TITLE     A genomic analysis of rat proteases and protease inhibitors
  JOURNAL   Genome Res 14 (4), 609-622 (2004)
   PUBMED   15060002
REFERENCE   9  (bases 1 to 485)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   10 (bases 1 to 485)
  AUTHORS   Harrington,J.J., Sherf,B., Rundlett,S., Jackson,P.D., Perry,R.,
            Cain,S., Leventhal,C., Thornton,M., Ramachandran,R.,
            Whittington,J., Lerner,L., Costanzo,D., McElligott,K., Boozer,S.,
            Mays,R., Smith,E., Veloso,N., Klika,A., Hess,J., Cothren,K., Lo,K.,
            Offenbacher,J., Danzig,J. and Ducar,M.
  TITLE     Creation of genome-wide protein expression libraries using random
            activation of gene expression
  JOURNAL   Nat Biotechnol 19 (5), 440-445 (2001)
   PUBMED   11329013
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from GQ504704.1 and AY358716.1.
            
            On Nov 23, 2018 this sequence version replaced NM_205841.3.
            
            Summary: The protein encoded by this gene is a Kazal-type serine
            protease inhibitor that acts on kallikrein-related peptidases in
            the skin. Two transcript variants the same protein have been found
            for this gene. [provided by RefSeq, Aug 2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 both encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC032003.1, BG191133.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMN03465402
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000325630.3/ ENSP00000324870.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-160               GQ504704.1         267-426
            161-485             AY358716.1         129-453
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q32"
     gene            1..485
                     /gene="SPINK6"
                     /gene_synonym="BUSI2; UNQ844"
                     /note="serine peptidase inhibitor Kazal type 6"
                     /db_xref="GeneID:404203"
                     /db_xref="HGNC:HGNC:29486"
                     /db_xref="MIM:615868"
     exon            1..113
                     /gene="SPINK6"
                     /gene_synonym="BUSI2; UNQ844"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..298
                     /gene="SPINK6"
                     /gene_synonym="BUSI2; UNQ844"
                     /note="protease inhibitor H; serine protease inhibitor
                     Kazal-type 6; acrosin inhibitor; kallikrein inhibitor"
                     /codon_start=1
                     /product="serine protease inhibitor Kazal-type 6
                     precursor"
                     /protein_id="NP_995313.2"
                     /db_xref="CCDS:CCDS34268.1"
                     /db_xref="GeneID:404203"
                     /db_xref="HGNC:HGNC:29486"
                     /db_xref="MIM:615868"
                     /translation="MKLSGMFLLLSLALFCFLTGVFSQGGQVDCGEFQDPKVYCTRES
                     NPHCGSDGQTYGNKCAFCKAIVKSGGKISLKHPGKC"
     exon            114..136
                     /gene="SPINK6"
                     /gene_synonym="BUSI2; UNQ844"
                     /inference="alignment:Splign:2.1.0"
     exon            137..252
                     /gene="SPINK6"
                     /gene_synonym="BUSI2; UNQ844"
                     /inference="alignment:Splign:2.1.0"
     exon            253..485
                     /gene="SPINK6"
                     /gene_synonym="BUSI2; UNQ844"
                     /inference="alignment:Splign:2.1.0"
     regulatory      462..467
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPINK6"
                     /gene_synonym="BUSI2; UNQ844"
                     /note="hexamer: AATAAA"
     polyA_site      485
                     /gene="SPINK6"
                     /gene_synonym="BUSI2; UNQ844"
                     /note="major polyA site"
ORIGIN      
        1 acagagaacc tcagctggac aaagcagcct tgatctgagt gagctaactg acacaatgaa
       61 actgtcaggc atgtttctgc tcctctctct ggctcttttc tgctttttaa caggtgtctt
      121 cagtcaggga ggacaggttg actgtggtga gttccaggac cccaaggtct actgcactcg
      181 ggaatctaac ccacactgtg gctctgatgg ccagacatat ggcaataaat gtgccttctg
      241 taaggccata gtgaaaagtg gtggaaagat tagcctaaag catcctggaa aatgctgagt
      301 taaagccaat gtttcttggt gacttgccag cttttgcagc cttcttttct cacttctgct
      361 tatacttttg ctggtggatt cctttaattc ataaagacat acctactctg cctgggtctt
      421 gaggagttca atgtatgtct atttctcttg attcacttgt caataaagta cattctgcaa
      481 aagca
//
