LOCUS       NM_006846               3651 bp    mRNA    linear   PRI 17-MAR-2024
DEFINITION  Homo sapiens serine peptidase inhibitor Kazal type 5 (SPINK5),
            transcript variant 2, mRNA.
ACCESSION   NM_006846
VERSION     NM_006846.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Chen,J., Lu,J., Chen,Z., Liu,Z., Sun,Y., He,S., Mi,Y., Gao,Y.,
            Shen,D. and Lin,Q.
  TITLE     SPINK5 inhibits esophageal squamous cell carcinoma metastasis via
            immune activity
  JOURNAL   J Gene Med 26 (3), e3667 (2024)
   PUBMED   38442944
  REMARK    GeneRIF: SPINK5 inhibits esophageal squamous cell carcinoma
            metastasis via immune activity.
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Petrova,E., Lopez-Gay,J.M., Fahrner,M., Leturcq,F., de
            Villartay,J.P., Barbieux,C., Gonschorek,P., Tsoi,L.C.,
            Gudjonsson,J.E., Schilling,O. and Hovnanian,A.
  TITLE     Comparative analyses of Netherton syndrome patients and Spink5
            conditional knock-out mice uncover disease-relevant pathways
  JOURNAL   Commun Biol 7 (1), 152 (2024)
   PUBMED   38316920
  REMARK    GeneRIF: Comparative analyses of Netherton syndrome patients and
            Spink5 conditional knock-out mice uncover disease-relevant
            pathways.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3651)
  AUTHORS   Sun,S., Su,G. and Zheng,X.
  TITLE     Inhibition of the Tumor Suppressor Gene SPINK5 via EHMT2 Induces
            the Oral Squamous Cell Carcinoma Development
  JOURNAL   Mol Biotechnol 66 (2), 208-221 (2024)
   PUBMED   37071303
  REMARK    GeneRIF: Inhibition of the Tumor Suppressor Gene SPINK5 via EHMT2
            Induces the Oral Squamous Cell Carcinoma Development.
REFERENCE   4  (bases 1 to 3651)
  AUTHORS   Frank,D.N., Giese,A.P.J., Hafren,L., Bootpetch,T.C., Yarza,T.K.L.,
            Steritz,M.J., Pedro,M., Labra,P.J., Daly,K.A., Tantoco,M.L.C.,
            Szeremeta,W., Reyes-Quintos,M.R.T., Ahankoob,N., Llanes,E.G.D.V.,
            Pine,H.S., Yousaf,S., Ir,D., Einarsdottir,E., de la Cruz,R.A.R.,
            Lee,N.R., Nonato,R.M.A., Robertson,C.E., Ong,K.M.C., Magno,J.P.M.,
            Chiong,A.N.E., Espiritu-Chiong,M.C., San Agustin,M.L., Cruz,T.L.G.,
            Abes,G.T., Bamshad,M.J., Cutiongco-de la Paz,E.M., Kere,J.,
            Nickerson,D.A., Mohlke,K.L., Riazuddin,S., Chan,A., Mattila,P.S.,
            Leal,S.M., Ryan,A.F., Ahmed,Z.M., Chonmaitree,T., Sale,M.M.,
            Chiong,C.M. and Santos-Cortez,R.L.P.
  TITLE     Otitis media susceptibility and shifts in the head and neck
            microbiome due to SPINK5 variants
  JOURNAL   J Med Genet 58 (7), 442-452 (2021)
   PUBMED   32709676
  REMARK    GeneRIF: Otitis media susceptibility and shifts in the head and
            neck microbiome due to SPINK5 variants.
REFERENCE   5  (bases 1 to 3651)
  AUTHORS   Furio,L. and Hovnanian,A.
  TITLE     Netherton syndrome: defective kallikrein inhibition in the skin
            leads to skin inflammation and allergy
  JOURNAL   Biol Chem 395 (9), 945-958 (2014)
   PUBMED   25153381
  REMARK    Review article
REFERENCE   6  (bases 1 to 3651)
  AUTHORS   Walley,A.J., Chavanas,S., Moffatt,M.F., Esnouf,R.M., Ubhi,B.,
            Lawrence,R., Wong,K., Abecasis,G.R., Jones,E.Y., Harper,J.I.,
            Hovnanian,A. and Cookson,W.O.
  TITLE     Gene polymorphism in Netherton and common atopic disease
  JOURNAL   Nat Genet 29 (2), 175-178 (2001)
   PUBMED   11544479
REFERENCE   7  (bases 1 to 3651)
  AUTHORS   Sprecher,E., Chavanas,S., DiGiovanna,J.J., Amin,S., Nielsen,K.,
            Prendiville,J.S., Silverman,R., Esterly,N.B., Spraker,M.K.,
            Guelig,E., de Luna,M.L., Williams,M.L., Buehler,B., Siegfried,E.C.,
            Van Maldergem,L., Pfendner,E., Bale,S.J., Uitto,J., Hovnanian,A.
            and Richard,G.
  TITLE     The spectrum of pathogenic mutations in SPINK5 in 19 families with
            Netherton syndrome: implications for mutation detection and first
            case of prenatal diagnosis
  JOURNAL   J Invest Dermatol 117 (2), 179-187 (2001)
   PUBMED   11511292
REFERENCE   8  (bases 1 to 3651)
  AUTHORS   Chavanas,S., Bodemer,C., Rochat,A., Hamel-Teillac,D., Ali,M.,
            Irvine,A.D., Bonafe,J.L., Wilkinson,J., Taieb,A., Barrandon,Y.,
            Harper,J.I., de Prost,Y. and Hovnanian,A.
  TITLE     Mutations in SPINK5, encoding a serine protease inhibitor, cause
            Netherton syndrome
  JOURNAL   Nat Genet 25 (2), 141-142 (2000)
   PUBMED   10835624
REFERENCE   9  (bases 1 to 3651)
  AUTHORS   Chavanas,S., Garner,C., Bodemer,C., Ali,M., Teillac,D.H.,
            Wilkinson,J., Bonafe,J.L., Paradisi,M., Kelsell,D.P., Ansai,Si.,
            Mitsuhashi,Y., Larregue,M., Leigh,I.M., Harper,J.I., Taieb,A.,
            Prost,Yd., Cardon,L.R. and Hovnanian,A.
  TITLE     Localization of the Netherton syndrome gene to chromosome 5q32, by
            linkage analysis and homozygosity mapping
  JOURNAL   Am J Hum Genet 66 (3), 914-921 (2000)
   PUBMED   10712206
REFERENCE   10 (bases 1 to 3651)
  AUTHORS   Magert,H.J., Standker,L., Kreutzmann,P., Zucht,H.D., Reinecke,M.,
            Sommerhoff,C.P., Fritz,H. and Forssmann,W.G.
  TITLE     LEKTI, a novel 15-domain type of human serine proteinase inhibitor
  JOURNAL   J Biol Chem 274 (31), 21499-21502 (1999)
   PUBMED   10419450
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DQ149927.1, AK307588.1,
            AK307648.1, AK025197.1, DR421881.1, AI341060.1 and AC116334.2.
            
            On Nov 30, 2019 this sequence version replaced NM_006846.3.
            
            Summary: This gene encodes a multidomain serine protease inhibitor
            that contains 15 potential inhibitory domains. The encoded
            preproprotein is proteolytically processed to generate multiple
            protein products, which may exhibit unique activities and
            specificities. These proteins may play a role in skin and hair
            morphogenesis, as well as anti-inflammatory and antimicrobial
            protection of mucous epithelia. Mutations in this gene may result
            in Netherton syndrome, a disorder characterized by ichthyosis,
            defective cornification, and atopy. This gene is present in a gene
            cluster on chromosome 5. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (2, also known as SPINK5f-l) lacks
            an alternate in-frame exon in the 3' coding region, compared to
            variant 1, resulting in a shorter protein (isoform b, also known as
            the LEKTIf-l isoform), compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ149927.1, AJ228139.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145893, SAMEA2147596
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000256084.8/ ENSP00000256084.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-864               DQ149927.1         9-872
            865-1220            AK307588.1         808-1163
            1221-2324           AK307648.1         1320-2423
            2325-2539           AK025197.1         242-456
            2540-3073           DQ149927.1         2548-3081
            3074-3303           DR421881.1         197-426
            3304-3623           AI341060.1         11-330              c
            3624-3631           DR421881.1         750-757
            3632-3651           AC116334.2         67325-67344
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q32"
     gene            1..3651
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /note="serine peptidase inhibitor Kazal type 5"
                     /db_xref="GeneID:11005"
                     /db_xref="HGNC:HGNC:15464"
                     /db_xref="MIM:605010"
     exon            1..120
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..3260
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /note="isoform b preproprotein is encoded by transcript
                     variant 2; lymphoepithelial Kazal-type-related inhibitor;
                     serine protease inhibitor Kazal-type 5; lympho-epithelial
                     Kazal-type-related inhibitor"
                     /codon_start=1
                     /product="serine protease inhibitor Kazal-type 5 isoform b
                     preproprotein"
                     /protein_id="NP_006837.2"
                     /db_xref="CCDS:CCDS43382.1"
                     /db_xref="GeneID:11005"
                     /db_xref="HGNC:HGNC:15464"
                     /db_xref="MIM:605010"
                     /translation="MKIATVSVLLPLALCLIQDAASKNEDQEMCHEFQAFMKNGKLFC
                     PQDKKFFQSLDGIMFINKCATCKMILEKEAKSQKRARHLARAPKATAPTELNCDDFKK
                     GERDGDFICPDYYEAVCGTDGKTYDNRCALCAENAKTGSQIGVKSEGECKSSNPEQDV
                     CSAFRPFVRDGRLGCTRENDPVLGPDGKTHGNKCAMCAELFLKEAENAKREGETRIRR
                     NAEKDFCKEYEKQVRNGRLFCTRESDPVRGPDGRMHGNKCALCAEIFKQRFSEENSKT
                     DQNLGKAEEKTKVKREIVKLCSQYQNQAKNGILFCTRENDPIRGPDGKMHGNLCSMCQ
                     AYFQAENEEKKKAEARARNKRESGKATSYAELCSEYRKLVRNGKLACTRENDPIQGPD
                     GKVHGNTCSMCEVFFQAEEEEKKKKEGKSRNKRQSKSTASFEELCSEYRKSRKNGRLF
                     CTRENDPIQGPDGKMHGNTCSMCEAFFQQEERARAKAKREAAKEICSEFRDQVRNGTL
                     ICTREHNPVRGPDGKMHGNKCAMCASVFKLEEEEKKNDKEEKGKVEAEKVKREAVQEL
                     CSEYRHYVRNGRLPCTRENDPIEGLDGKIHGNTCSMCEAFFQQEAKEKERAEPRAKVK
                     REAEKETCDEFRRLLQNGKLFCTRENDPVRGPDGKTHGNKCAMCKAVFQKENEERKRK
                     EEEDQRNAAGHGSSGGGGGNTQDECAEYREQMKNGRLSCTRESDPVRDADGKSYNNQC
                     TMCKAKLEREAERKNEYSRSRSNGTGSESGKDTCDEFRSQMKNGKLICTRESDPVRGP
                     DGKTHGNKCTMCKEKLEREAAEKKKKEDEDRSNTGERSNTGERSNDKEDLCREFRSMQ
                     RNGKLICTRENNPVRGPYGKMHINKCAMCQSIFDREANERKKKDEEKSSSKPSNNAKD
                     ECSEFRNYIRNNELICPRENDPVHGADGKFYTNKCYMCRAVFLTEALERAKLQEKPSH
                     VRASQEEDSPDSFSSLDSEMCKDYRVLPRIGYLCPKDLKPVCGDDGQTYNNPCMLCHE
                     NLIRQTNTHIRSTGKCEESSTPGTTAASMPPSDE"
     exon            121..146
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            147..274
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            275..347
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            348..475
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            476..539
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            540..667
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            668..731
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            732..859
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            860..947
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1075
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1157
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1285
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1367
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1495
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1544
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1672
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1757
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1885
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1952
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2080
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2177
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            2178..2305
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..2378
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            2379..2506
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            2507..2603
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..2731
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..2804
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..2932
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            2933..3029
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            3030..3160
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            3161..3251
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     exon            3252..3651
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3531..3536
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /note="hexamer: AATAAA"
     polyA_site      3552
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /note="major polyA site"
     regulatory      3615..3620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
                     /note="hexamer: AATAAA"
     polyA_site      3651
                     /gene="SPINK5"
                     /gene_synonym="LEKTI; LETKI; NETS; NS; VAKTI"
ORIGIN      
        1 agtcatactg caccagctga gcaatgcatg gagtggacct gtaggcgact tgcatcgtct
       61 tcaacatgaa gatagccaca gtgtcagtgc ttctgccctt ggctctttgc ctcatacaag
      121 atgctgccag taagaatgaa gatcaggaaa tgtgccatga atttcaggca tttatgaaaa
      181 atggaaaact gttctgtccc caggataaga aattttttca aagtcttgat ggaataatgt
      241 tcatcaataa atgtgccacg tgcaaaatga tactggaaaa agaagcaaaa tcacagaaga
      301 gggccaggca tttagcaaga gctcccaagg ctactgcccc aacagagctg aattgtgatg
      361 attttaaaaa aggagaaaga gatggggatt ttatctgtcc tgattattat gaagctgttt
      421 gtggcacaga tgggaaaaca tatgacaaca gatgtgcact gtgtgctgag aatgcgaaaa
      481 ccgggtccca aattggtgta aaaagtgaag gggaatgtaa gagcagtaat ccagagcagg
      541 atgtatgcag tgcttttcgg ccctttgtta gagatggaag acttggatgc acaagggaaa
      601 atgatcctgt tcttggtcct gatgggaaga cgcatggcaa taagtgtgca atgtgtgctg
      661 agctgttttt aaaagaagct gaaaatgcca agcgagaggg tgaaactaga attcgacgaa
      721 atgctgaaaa ggatttttgc aaggaatatg aaaaacaagt gagaaatgga aggctttttt
      781 gtacacggga gagtgatcca gtccgtggcc ctgacggcag gatgcatggc aacaaatgtg
      841 ccctgtgtgc tgaaattttc aagcagcgtt tttcagagga aaacagtaaa acagatcaaa
      901 atttgggaaa agctgaagaa aaaactaaag ttaaaagaga aattgtgaaa ctctgcagtc
      961 aatatcaaaa tcaggcaaag aatggaatac ttttctgtac cagagaaaat gaccctattc
     1021 gtggtccaga tgggaaaatg catggcaact tgtgttccat gtgtcaagcc tacttccaag
     1081 cagaaaatga agaaaagaaa aaggctgaag cacgagctag aaacaaaaga gaatctggaa
     1141 aagcaacctc atatgcagag ctttgcagtg aatatcgaaa gcttgtgagg aacggaaaac
     1201 ttgcttgcac cagagagaac gatcctatcc agggcccaga tgggaaagtg catggcaaca
     1261 cctgctccat gtgtgaggtc ttcttccaag cagaagaaga agaaaagaaa aagaaggaag
     1321 gtaaatcaag aaacaaaaga caatctaaga gtacagcttc ctttgaggag ttgtgtagtg
     1381 aataccgcaa atccaggaaa aacggacggc ttttttgcac cagagagaat gaccccatcc
     1441 agggcccaga tggaaaaatg catggcaaca cctgctccat gtgtgaggcc ttctttcaac
     1501 aagaagaaag agcaagagca aaggctaaaa gagaagctgc aaaggaaatc tgcagtgaat
     1561 ttcgggacca agtgaggaat ggaacactta tatgcaccag ggagcataat cctgtccgtg
     1621 gcccagatgg caaaatgcat ggaaacaagt gtgccatgtg tgccagtgtg ttcaaacttg
     1681 aagaagaaga gaagaaaaat gataaagaag aaaaagggaa agtcgaggct gaaaaagtta
     1741 agagagaagc agttcaggag ctgtgcagtg aatatcgtca ttatgtgagg aatggacgac
     1801 tcccctgtac cagagagaat gatcctattg agggtctaga tgggaaaatc cacggcaaca
     1861 cctgctccat gtgtgaagcc ttcttccagc aagaagcaaa agaaaaagaa agagctgaac
     1921 ccagagcaaa agtcaaaaga gaagctgaaa aggagacatg cgatgaattt cggagacttt
     1981 tgcaaaatgg aaaacttttc tgcacaagag aaaatgatcc tgtgcgtggc ccagatggca
     2041 agacccatgg caacaagtgt gccatgtgta aggcagtctt ccagaaagaa aatgaggaaa
     2101 gaaagaggaa agaagaggaa gatcagagaa atgctgcagg acatggttcc agtggtggtg
     2161 gaggaggaaa cactcaggac gaatgtgctg agtatcggga acaaatgaaa aatggaagac
     2221 tcagctgtac tcgggagagt gatcctgtac gtgatgctga tggcaaatcg tacaacaatc
     2281 agtgtaccat gtgtaaagca aaattggaaa gagaagcaga gagaaaaaat gagtattctc
     2341 gctccagatc aaatgggact ggatcagaat cagggaagga tacatgtgat gagtttagaa
     2401 gccaaatgaa aaatggaaaa ctcatctgca ctcgagaaag tgaccctgtc cggggtccag
     2461 atggcaagac acatggcaat aagtgtacta tgtgtaagga aaaactggaa agggaagcag
     2521 ctgaaaaaaa aaagaaagag gatgaagaca ggagcaatac aggagaaagg agcaatacag
     2581 gagaaaggag caatgacaaa gaggatctgt gtcgtgaatt tcgaagcatg cagagaaatg
     2641 gaaagcttat ctgcaccaga gaaaataacc ctgttcgagg cccatatggc aagatgcaca
     2701 tcaataaatg tgctatgtgt cagagcatct ttgatcgaga agctaatgaa agaaaaaaga
     2761 aagatgaaga gaaatcaagt agcaagccct caaataatgc aaaggatgag tgcagtgaat
     2821 ttcgaaacta tataaggaac aatgaactca tctgccctag agagaatgac ccagtgcacg
     2881 gtgctgatgg aaagttctat acaaacaagt gctacatgtg cagagctgtc tttctaacag
     2941 aagctttgga aagggcaaag cttcaagaaa agccatccca tgttagagct tctcaagagg
     3001 aagacagccc agactctttc agttctctgg attctgagat gtgcaaagac taccgagtat
     3061 tgcccaggat aggttatctt tgtccaaagg atttaaagcc tgtctgtggt gacgatggcc
     3121 aaacctacaa caatccttgc atgctctgtc atgaaaacct gatacgccaa acaaatacac
     3181 acatccgcag tacagggaag tgtgaggaga gcagcacccc aggaaccacc gcagccagca
     3241 tgcccccgtc tgacgaatga caggaagatt gttgaaagcc atgagggaaa aaataaaccc
     3301 cagttctgaa tcacctacct tcaccatctg tatatacaaa gaattcttcg gagcttgtct
     3361 tatttgctat agaaaacaat acagagcttt tgggaatgga ctcactgatt ttcagtcttt
     3421 tccatctctt tcctcctaga ctctgtgatc tgagggtata aagacatctc caccaagtct
     3481 gagccctcaa aatgtcctga ttacaatgct gtctgtccaa ctgcctgttc aataaaagta
     3541 aactcagcag aacacccttt ctgggatttc tttgtcacta tctggataat agatatttgc
     3601 ttttaaagaa actgaataaa ctctaccctt ttgtcttttt gtgttgctaa a
//
