LOCUS       NM_003121               3792 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens Spi-B transcription factor (SPIB), transcript variant
            1, mRNA.
ACCESSION   NM_003121
VERSION     NM_003121.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ling,B., Ye,G., Qin,C., Liao,X., Yang,R., Su,L. and Qi,G.
  TITLE     IGSF10 inhibits the metastasis of lung adenocarcinoma via the
            Spi-B/Integrin-beta1 signaling pathway
  JOURNAL   J Biochem Mol Toxicol 38 (4), e23693 (2024)
   PUBMED   38622980
  REMARK    GeneRIF: IGSF10 inhibits the metastasis of lung adenocarcinoma via
            the Spi-B/Integrin-beta1 signaling pathway.
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Gu,W., Qi,G. and Chen,L.
  TITLE     SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells
            by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT
            Pathway
  JOURNAL   Iran J Immunol 20 (3), 335-347 (2023)
   PUBMED   37452634
  REMARK    GeneRIF: SPIB Knockdown Inhibits the Immune Escape of Ovarian
            Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating
            the JAK/STAT Pathway.
REFERENCE   3  (bases 1 to 3792)
  AUTHORS   Wu,F., Tian,F., Qin,C., Qin,X., Zeng,W., Liu,X., Chen,C. and Lin,Y.
  TITLE     Peroxiredoxin2 regulates trophoblast proliferation and migration
            through SPIB-HDAC2 pathway
  JOURNAL   Exp Cell Res 422 (1), 113428 (2023)
   PUBMED   36400181
  REMARK    GeneRIF: Peroxiredoxin2 regulates trophoblast proliferation and
            migration through SPIB-HDAC2 pathway.
REFERENCE   4  (bases 1 to 3792)
  AUTHORS   Ding,M., Li,Q., Tan,X., Zhang,L., Tan,J. and Zheng,L.
  TITLE     Comprehensive pan-cancer analysis reveals the prognostic value and
            immunological role of SPIB
  JOURNAL   Aging (Albany NY) 14 (15), 6338-6357 (2022)
   PUBMED   35969172
  REMARK    GeneRIF: Comprehensive pan-cancer analysis reveals the prognostic
            value and immunological role of SPIB.
REFERENCE   5  (bases 1 to 3792)
  AUTHORS   Mohsin,M., Singh,P., Khan,S., Verma,A.K., Jha,R., Alsahli,M.A.,
            Rahmani,A.H., Almatroodi,S.A., Alrumaihi,F., Kaprwan,N., Dev,K.,
            Dohare,R. and Syed,M.A.
  TITLE     Integrated transcriptomic and regulatory network analyses uncovers
            the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis
  JOURNAL   Sci Rep 12 (1), 11963 (2022)
   PUBMED   35831411
  REMARK    GeneRIF: Integrated transcriptomic and regulatory network analyses
            uncovers the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3792)
  AUTHORS   Yamamoto,H., Kihara-Negishi,F., Yamada,T., Suzuki,M., Nakano,T. and
            Oikawa,T.
  TITLE     Interaction between the hematopoietic Ets transcription factor
            Spi-B and the coactivator CREB-binding protein associated with
            negative cross-talk with c-Myb
  JOURNAL   Cell Growth Differ 13 (2), 69-75 (2002)
   PUBMED   11864910
REFERENCE   7  (bases 1 to 3792)
  AUTHORS   Nagy,M., Chapuis,B. and Matthes,T.
  TITLE     Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and
            oct-2 in normal human plasma cells and in multiple myeloma cells
  JOURNAL   Br J Haematol 116 (2), 429-435 (2002)
   PUBMED   11841448
REFERENCE   8  (bases 1 to 3792)
  AUTHORS   Rao,S., Matsumura,A., Yoon,J. and Simon,M.C.
  TITLE     SPI-B activates transcription via a unique proline, serine, and
            threonine domain and exhibits DNA binding affinity differences from
            PU.1
  JOURNAL   J Biol Chem 274 (16), 11115-11124 (1999)
   PUBMED   10196196
REFERENCE   9  (bases 1 to 3792)
  AUTHORS   Ray-Gallet,D., Tavitian,A. and Moreau-Gachelin,F.
  TITLE     An alternatively spliced isoform of the Spi-B transcription factor
  JOURNAL   Biochem Biophys Res Commun 223 (2), 257-263 (1996)
   PUBMED   8670269
REFERENCE   10 (bases 1 to 3792)
  AUTHORS   Ray,D., Bosselut,R., Ghysdael,J., Mattei,M.G., Tavitian,A. and
            Moreau-Gachelin,F.
  TITLE     Characterization of Spi-B, a transcription factor related to the
            putative oncoprotein Spi-1/PU.1
  JOURNAL   Mol Cell Biol 12 (10), 4297-4304 (1992)
   PUBMED   1406622
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC020909.9.
            
            On Nov 23, 2018 this sequence version replaced NM_003121.4.
            
            Summary: The protein encoded by this gene is a transcriptional
            activator that binds to the PU-box (5'-GAGGAA-3') and acts as a
            lymphoid-specific enhancer. Four transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Sep 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK225720.1, AK225225.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000595883.6/ ENSP00000471921.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AC020909.9         17244-17291
            49-76               AC020909.9         18252-18279
            77-149              AC020909.9         20779-20851
            150-364             AC020909.9         21129-21343
            365-515             AC020909.9         21911-22061
            516-3792            AC020909.9         26344-29620
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..3792
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /note="Spi-B transcription factor"
                     /db_xref="GeneID:6689"
                     /db_xref="HGNC:HGNC:11242"
                     /db_xref="MIM:606802"
     exon            1..48
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..814
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transcription factor Spi-B; Spi-B transcription factor
                     (Spi-1/PU.1 related)"
                     /codon_start=1
                     /product="transcription factor Spi-B isoform 1"
                     /protein_id="NP_003112.2"
                     /db_xref="CCDS:CCDS33080.1"
                     /db_xref="GeneID:6689"
                     /db_xref="HGNC:HGNC:11242"
                     /db_xref="MIM:606802"
                     /translation="MLALEAAQLDGPHFSCLYPDGVFYDLDSCKHSSYPDSEGAPDSL
                     WDWTVAPPVPATPYEAFDPAAAAFSHPQAAQLCYEPPTYSPAGNLELAPSLEAPGPGL
                     PAYPTENFASQTLVPPAYAPYPSPVLSEEEDLPLDSPALEVSDSESDEALVAGPEGKG
                     SEAGTRKKLRLYQFLLGLLTRGDMRECVWWVEPGAGVFQFSSKHKELLARRWGQQKGN
                     RKRMTYQKLARALRNYAKTGEIRKVKRKLTYQFDSALLPAVRRA"
     exon            49..76
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /inference="alignment:Splign:2.1.0"
     exon            77..149
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /inference="alignment:Splign:2.1.0"
     exon            150..364
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /inference="alignment:Splign:2.1.0"
     exon            365..515
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /inference="alignment:Splign:2.1.0"
     exon            516..3792
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1471
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /note="major polyA site"
     regulatory      3502..3507
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /note="hexamer: AATAAA"
     regulatory      3507..3512
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /note="hexamer: AATAAG"
     polyA_site      3525
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
     polyA_site      3531
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
     regulatory      3775..3780
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
                     /note="hexamer: AATAAA"
     polyA_site      3792
                     /gene="SPIB"
                     /gene_synonym="SPI-B"
ORIGIN      
        1 ggcaaacagc ccgcccggca ccaccatgct cgccctggag gctgcacagc tcgacgggcc
       61 acacttcagc tgtctgtacc cagatggcgt cttctatgac ctggacagct gcaagcattc
      121 cagctaccct gattcagagg gggctcctga ctccctgtgg gactggactg tggccccacc
      181 tgtcccagcc accccctatg aagccttcga cccggcagca gccgctttta gccaccccca
      241 ggctgcccag ctctgctacg aaccccccac ctacagccct gcagggaacc tcgaactggc
      301 ccccagcctg gaggccccgg ggcctggcct ccctgcatac cccacggaga acttcgctag
      361 ccagaccctg gttcccccgg catatgcccc gtaccccagc cctgtgctat cagaggagga
      421 agacttaccg ttggacagcc ctgccctgga ggtctcggac agcgagtcgg atgaggccct
      481 cgtggctggc cccgagggga agggatccga ggcagggact cgcaagaagc tgcgcctgta
      541 ccagttcctg ctggggctac tgacgcgcgg ggacatgcgt gagtgcgtgt ggtgggtgga
      601 gccaggcgcc ggcgtcttcc agttctcctc caagcacaag gaactcctgg cgcgccgctg
      661 gggccagcag aaggggaacc gcaagcgcat gacctaccag aagctggcgc gcgccctccg
      721 aaactacgcc aagaccggcg agatccgcaa ggtcaagcgc aagctcacct accagttcga
      781 cagcgcgctg ctgcctgcag tccgccgggc ctgagcacac ccgaggctcc cacctgcgga
      841 gccgctgggg gacctcacgt cccagccagg atccccctgg aagaaaaagg gcgtccccac
      901 actctaggtg ataggactta cgcatcccca ccttttgggg taaggggagt gctgccctgc
      961 cataatcccc aagcccagcc cgggcctgtc tgggattccc cacttgtgcc tggggtcctc
     1021 tgggatttct ttgtcatgta cagactccct gggatcctca tgttttgggt gacaggacct
     1081 atggaccact atactcgggg aggcagggta gcagttcttc cagaatccca agagcttctc
     1141 tgggattttc ttgtgatatc tgattcccca gtgaggcctg ggacgttttt aagatcgctg
     1201 tgtgtctgta aaccctgaat ctcatctggg gtgggggccc tgctggcaac cctgagccct
     1261 gtccaaggtt ccctcttgtc agatctgaga tttcctagtt atgtctgggg ccctctggga
     1321 gctgttatca tctcagatct cttcgcccat ctatggctgt gttgtcacat ctgtcccctc
     1381 atttttgaga tcccccaatt ctctggaact attctgctgc ccctttttat gtgtctggag
     1441 ttccccaatc acatctaggg ctcctccaag atccttttgt catgtctgaa atcactcttg
     1501 agaggtctgg ggtggaggat ggggagtcag tgaaatgtgt catgtctggg ccctgtcagg
     1561 gacacccttg ttatatctgg gatcctccaa tcacatctga gacctcctag gctctccatc
     1621 tgatatgccc tttcagggac cccacaaaga ctgagttctc atggggatcc tacccttcct
     1681 agtgccactc cctatggcca tgctgaagac cactctggcc acgcgactga ttttgggtga
     1741 tcatggcagc tccccaccca tgtcatttct aaccagaagt ctcaaggtcg tcacccccct
     1801 gccccccaac cgaggccccg gtcgctggtg gtggtctctt tagtgcactg tagcacttgg
     1861 tggtggaggt gtgagggatc cacattaaca gcaggccatc agctgggcaa tggctcacac
     1921 ctgtaatccc agcactttgg gaggcgaggc agggggaatg gcttgaaccc aggcattcaa
     1981 gaccagcctg ggcaacataa tgagacctcg tctctacaaa acataacaaa aacaattagc
     2041 cgagcgtggg ggtgaacacc tgtggtccca gctgctcagg aggctgaggt gggaggatct
     2101 cttgagccca ggaagtagga ggctgtagtg agctgtaatc gtgccactgc actccagcct
     2161 gggcgacaga gtgagacacc gtcttaaaaa caaaaacaag gccgggcacg gtggctcatg
     2221 cctgttgtcc cagcactttg ggaggccgag gcaggcggat cacgaggtcg agagatcgag
     2281 accatcctgg ccaacatggt gaaaccctgt ctctactaaa aatacagaaa ttagctgggc
     2341 gtggtggcac gtgcctgtag tcccagctac tcgggaggct gaggcaagag aatcgcttga
     2401 acgtgggagg cagaggttgc agtgagccta gattgtgcca ctgcactcca gcctggggga
     2461 cagagcgaga ctccgtctga aaataaaaac aacaaaaaca gcagaccatt caaaataggg
     2521 agactttgca taatccagat ttctgccttc acttaaaact ttggacggtc tggagagagt
     2581 cggccagttt tcggtggggg gtggggagct ggaacaggac agtagccttt cctaatgagg
     2641 catttgttct ccaatctgcc ccagtcgctg ccatccctgg ctatctcacc ctagcagctt
     2701 ctcaagcctg ttggctttag accactgtat aaacccagct ggaactgaag cctgggtgga
     2761 ctatggagcc ctggttggga cccccaggga gtcaaaggct gcgggccaag aggccagagg
     2821 tccttgagcc tgggtgggca ggtggatcta gggtgcatga cttgctgctt cccaacctta
     2881 gtttgtccct tctgtgaaaa agggagagaa ggaggaggaa gatctcaaaa agactttcca
     2941 gcccagtgcg gtggctcacg cctgtaatcc cagcactttg ggaggccgat gcaggtggat
     3001 cacctgaggt aggagttcaa gaccagcctg accaacatag tgaagcccct tctctactaa
     3061 aaatacaaaa ttagctgggc gtggtggcat gtgcctgtac tcccagctac ttgggaggct
     3121 gaggcaggag aatcgcttga acctgggagg cggaggttgt agtgagctga gatcacacca
     3181 ctgcacacca gcctgggcga caagagcgaa actccgtctc aaaaaaaaaa aactgttgca
     3241 gccccgttga gcctttgaca ccgcctgaaa tccaccccac tcccaggagg aggaggagga
     3301 aggaatgcca atgacctaga gacacgagaa gtccatgtgg aggcacacag cagctgatgg
     3361 cagagcccag gctgggacct gcccttaaga gaatgagtgg gaagggggag ggaggaaggg
     3421 caggtaaaac gtcctcccca gggccccctg caacggggaa ggtacttttt acaaaagcta
     3481 tcattgtcac cctaaatgtg gaataaaata agatgcatcg acgtagacaa acctcctggg
     3541 accttttgtc agggactgca atcctgcccc tccactgagg ccgctggctc tcagagacac
     3601 cgtgacatca cgggtgatga tgagaggagt tcaaagagag aattatatgc tggcgcggtg
     3661 gctctgtaat cccaacactt tggggggcca aggcaggagg atcgcttgag tacaggagtt
     3721 tgaaaccagc ctgggcaaga tagtgagatc cccttcccac ccgtctacaa aaaaaataaa
     3781 aaattagcgg gg
//
