LOCUS       NM_003120               1374 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens Spi-1 proto-oncogene (SPI1), transcript variant 2,
            mRNA.
ACCESSION   NM_003120
VERSION     NM_003120.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Liu,Y., Dong,M., Jia,Y., Yang,D., Hui,Y. and Yang,X.
  TITLE     SPI1-mediated transcriptional activation of CEP55 promotes the
            malignant growth of triple-negative breast cancer and M2 macrophage
            polarization
  JOURNAL   Pathol Res Pract 262, 155544 (2024)
   PUBMED   39197215
  REMARK    GeneRIF: SPI1-mediated transcriptional activation of CEP55 promotes
            the malignant growth of triple-negative breast cancer and M2
            macrophage polarization.
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Virgilio,M.C., Ramnani,B., Chen,T., Disbennett,W.M., Lubow,J.,
            Welch,J.D. and Collins,K.L.
  TITLE     HIV-1 Vpr combats the PU.1-driven antiviral response in primary
            human macrophages
  JOURNAL   Nat Commun 15 (1), 5514 (2024)
   PUBMED   38951492
  REMARK    GeneRIF: HIV-1 Vpr combats the PU.1-driven antiviral response in
            primary human macrophages.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1374)
  AUTHORS   Liu,J., Jiang,X., Liu,K., Deng,J., Qiu,Y., Wei,W. and Yang,C.
  TITLE     Role of LINC00240 on T-helper 9 differentiation in allergic
            rhinitis through influencing DNMT1-dependent methylation of PU.1
  JOURNAL   Immunol Res 72 (2), 197-211 (2024)
   PUBMED   37966708
  REMARK    GeneRIF: Role of LINC00240 on T-helper 9 differentiation in
            allergic rhinitis through influencing DNMT1-dependent methylation
            of PU.1.
REFERENCE   4  (bases 1 to 1374)
  AUTHORS   Mallorson,R., Miyagi,E., Kao,S., Sukegawa,S., Saito,H.,
            Fabryova,H., Morellatto Ruggieri,L., Mediouni,S., Valente,S.T. and
            Strebel,K.
  TITLE     Transcriptional regulation of the HIV-1 inhibitory factor human
            mannose receptor 1 by the myeloid-specific transcription factor
            PU.1
  JOURNAL   J Virol 98 (1), e0170223 (2024)
   PUBMED   38078733
  REMARK    GeneRIF: Transcriptional regulation of the HIV-1 inhibitory factor
            human mannose receptor 1 by the myeloid-specific transcription
            factor PU.1.
REFERENCE   5  (bases 1 to 1374)
  AUTHORS   Wenbo,D., Yifu,H. and Li,K.
  TITLE     SPI1 Regulates the Progression of Ankylosing Spondylitis by
            Modulating TLR5 via NF-kappaB Signaling
  JOURNAL   Inflammation 46 (5), 1697-1708 (2023)
   PUBMED   37277671
  REMARK    GeneRIF: SPI1 Regulates the Progression of Ankylosing Spondylitis
            by Modulating TLR5 via NF-kappaB Signaling.
REFERENCE   6  (bases 1 to 1374)
  AUTHORS   Nagulapalli,S., Pongubala,J.M. and Atchison,M.L.
  TITLE     Multiple proteins physically interact with PU.1. Transcriptional
            synergy with NF-IL6 beta (C/EBP delta, CRP3)
  JOURNAL   J Immunol 155 (9), 4330-4338 (1995)
   PUBMED   7594592
REFERENCE   7  (bases 1 to 1374)
  AUTHORS   Chen,H., Ray-Gallet,D., Zhang,P., Hetherington,C.J., Gonzalez,D.A.,
            Zhang,D.E., Moreau-Gachelin,F. and Tenen,D.G.
  TITLE     PU.1 (Spi-1) autoregulates its expression in myeloid cells
  JOURNAL   Oncogene 11 (8), 1549-1560 (1995)
   PUBMED   7478579
REFERENCE   8  (bases 1 to 1374)
  AUTHORS   Nguyen,V.C., Ray,D., Gross,M.S., de Tand,M.F., Frezal,J. and
            Moreau-Gachelin,F.
  TITLE     Localization of the human oncogene SPI1 on chromosome 11, region
            p11.22
  JOURNAL   Hum Genet 84 (6), 542-546 (1990)
   PUBMED   2338340
REFERENCE   9  (bases 1 to 1374)
  AUTHORS   Ray,D., Culine,S., Tavitain,A. and Moreau-Gachelin,F.
  TITLE     The human homologue of the putative proto-oncogene Spi-1:
            characterization and expression in tumors
  JOURNAL   Oncogene 5 (5), 663-668 (1990)
   PUBMED   1693183
  REMARK    Erratum:[Oncogene 1990 Oct;5(10):1611-2]
REFERENCE   10 (bases 1 to 1374)
  AUTHORS   Klemsz,M.J., McKercher,S.R., Celada,A., Van Beveren,C. and
            Maki,R.A.
  TITLE     The macrophage and B cell-specific transcription factor PU.1 is
            related to the ets oncogene
  JOURNAL   Cell 61 (1), 113-124 (1990)
   PUBMED   2180582
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP271276.1, X52056.1 and
            BM561863.1.
            
            On Jun 2, 2019 this sequence version replaced NM_003120.2.
            
            Summary: This gene encodes an ETS-domain transcription factor that
            activates gene expression during myeloid and B-lymphoid cell
            development. The nuclear protein binds to a purine-rich sequence
            known as the PU-box found near the promoters of target genes, and
            regulates their expression in coordination with other transcription
            factors and cofactors. The protein can also regulate alternative
            splicing of target genes. Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1,
            resulting in a shorter protein (isoform 2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X52056.1, SRR14038193.3619799.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378538.8/ ENSP00000367799.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-397               BP271276.1         30-426
            398-1025            X52056.1           397-1024
            1026-1374           BM561863.1         293-641
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p11.2"
     gene            1..1374
                     /gene="SPI1"
                     /gene_synonym="AGM10; OF; PU.1; SFPI1; SPI-1; SPI-A"
                     /note="Spi-1 proto-oncogene"
                     /db_xref="GeneID:6688"
                     /db_xref="HGNC:HGNC:11241"
                     /db_xref="MIM:165170"
     exon            1..239
                     /gene="SPI1"
                     /gene_synonym="AGM10; OF; PU.1; SFPI1; SPI-1; SPI-A"
                     /inference="alignment:Splign:2.1.0"
     CDS             195..1007
                     /gene="SPI1"
                     /gene_synonym="AGM10; OF; PU.1; SFPI1; SPI-1; SPI-A"
                     /note="isoform 2 is encoded by transcript variant 2;
                     hematopoietic transcription factor PU.1; 31 kDa
                     transforming protein; spleen focus forming virus (SFFV)
                     proviral integration oncogene spi1"
                     /codon_start=1
                     /product="transcription factor PU.1 isoform 2"
                     /protein_id="NP_003111.2"
                     /db_xref="CCDS:CCDS7933.2"
                     /db_xref="GeneID:6688"
                     /db_xref="HGNC:HGNC:11241"
                     /db_xref="MIM:165170"
                     /translation="MLQACKMEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGE
                     SHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVP
                     PHPSLGHQVSYLPRMCLQYPSLSPAQPSSDEEEGERQSPPLEVSDGEADGLEPGPGLL
                     PGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKG
                     NRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRHPPH"
     exon            240..336
                     /gene="SPI1"
                     /gene_synonym="AGM10; OF; PU.1; SFPI1; SPI-1; SPI-A"
                     /inference="alignment:Splign:2.1.0"
     exon            337..524
                     /gene="SPI1"
                     /gene_synonym="AGM10; OF; PU.1; SFPI1; SPI-1; SPI-A"
                     /inference="alignment:Splign:2.1.0"
     exon            525..687
                     /gene="SPI1"
                     /gene_synonym="AGM10; OF; PU.1; SFPI1; SPI-1; SPI-A"
                     /inference="alignment:Splign:2.1.0"
     exon            688..1374
                     /gene="SPI1"
                     /gene_synonym="AGM10; OF; PU.1; SFPI1; SPI-1; SPI-A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1354..1359
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPI1"
                     /gene_synonym="AGM10; OF; PU.1; SFPI1; SPI-1; SPI-A"
                     /note="hexamer: AGTAAA"
     polyA_site      1374
                     /gene="SPI1"
                     /gene_synonym="AGM10; OF; PU.1; SFPI1; SPI-1; SPI-A"
                     /note="major polyA site"
ORIGIN      
        1 aaaatcagga acttgtgctg gccctgcaat gtcaagggag ggggctcacc cagggctcct
       61 gtagctcagg gggcaggcct gagccctgca cccgccccac gaccgtccag cccctgacgg
      121 ggcaccccat cctgaggggc tctgcattgg cccccaccga ggcaggggat ctgaccgact
      181 cggagcccgg ctggatgtta caggcgtgca aaatggaagg gtttcccctc gtcccccctc
      241 catcagaaga cctggtgccc tatgacacgg atctatacca acgccaaacg cacgagtatt
      301 acccctatct cagcagtgat ggggagagcc atagcgacca ttactgggac ttccaccccc
      361 accacgtgca cagcgagttc gagagcttcg ccgagaacaa cttcacggag ctccagagcg
      421 tgcagccccc gcagctgcag cagctctacc gccacatgga gctggagcag atgcacgtcc
      481 tcgatacccc catggtgcca ccccatccca gtcttggcca ccaggtctcc tacctgcccc
      541 ggatgtgcct ccagtaccca tccctgtccc cagcccagcc cagctcagat gaggaggagg
      601 gcgagcggca gagcccccca ctggaggtgt ctgacggcga ggcggatggc ctggagcccg
      661 ggcctgggct cctgcctggg gagacaggca gcaagaagaa gatccgcctg taccagttcc
      721 tgttggacct gctccgcagc ggcgacatga aggacagcat ctggtgggtg gacaaggaca
      781 agggcacctt ccagttctcg tccaagcaca aggaggcgct ggcgcaccgc tggggcatcc
      841 agaagggcaa ccgcaagaag atgacctacc agaagatggc gcgcgcgctg cgcaactacg
      901 gcaagacggg cgaggtcaag aaggtgaaga agaagctcac ctaccagttc agcggcgaag
      961 tgctgggccg cgggggcctg gccgagcggc gccacccgcc ccactgagcc cgcagccccc
     1021 gccgggcccc gccaggcctc cccgctggcc atagcattaa gccctcgccc ggcccggaca
     1081 cagggaggac gctcccgggg cccagaggca ggactgtggc gggccgggcc tcgcctcacc
     1141 cgccccctcc ccccactcca ggccccctcc acatcccgct tcgcctccct ccaggactcc
     1201 accccggctc ccggacgcca gctgggcgtc agaccccacc ggggcaacct tgcagaggac
     1261 gacccggggt actgccttgg gagtctcaag tccgtatgta aatcagatct cccctctcac
     1321 ccctcccacc cattaacctc ctcccaaaaa acaagtaaag ttattctcaa tcca
//
