LOCUS       NM_001142644            6954 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP),
            transcript variant 1, mRNA.
ACCESSION   NM_001142644
VERSION     NM_001142644.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Long,J., Yao,Z., Sui,Y. and Fang,S.
  TITLE     SphK1 Promotes Cancer Progression through Activating JAK/STAT
            Pathway and Up-Regulating S1PR1 Expression in Colon Cancer Cells
  JOURNAL   Anticancer Agents Med Chem 22 (2), 254-260 (2022)
   PUBMED   33797381
  REMARK    GeneRIF: SphK1 Promotes Cancer Progression through Activating
            JAK/STAT Pathway and Up-Regulating S1PR1 Expression in Colon Cancer
            Cells.
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Ghazaly,E.A., Miraki-Moud,F., Smith,P., Gnanaranjan,C., Koniali,L.,
            Oke,A., Saied,M.H., Petty,R., Matthews,J., Stronge,R., Joel,S.P.,
            Young,B.D., Gribben,J. and Taussig,D.C.
  TITLE     Repression of sphingosine kinase (SK)-interacting protein (SKIP) in
            acute myeloid leukemia diminishes SK activity and its re-expression
            restores SK function
  JOURNAL   J Biol Chem 295 (16), 5496-5508 (2020)
   PUBMED   32161116
  REMARK    GeneRIF: Repression of sphingosine kinase (SK)-interacting protein
            (SKIP) in acute myeloid leukemia diminishes SK activity and its
            re-expression restores SK function.
REFERENCE   3  (bases 1 to 6954)
  AUTHORS   Wade,T.D., Gordon,S., Medland,S., Bulik,C.M., Heath,A.C.,
            Montgomery,G.W. and Martin,N.G.
  TITLE     Genetic variants associated with disordered eating
  JOURNAL   Int J Eat Disord 46 (6), 594-608 (2013)
   PUBMED   23568457
REFERENCE   4  (bases 1 to 6954)
  AUTHORS   Saied,M.H., Marzec,J., Khalid,S., Smith,P., Down,T.A., Rakyan,V.K.,
            Molloy,G., Raghavan,M., Debernardi,S. and Young,B.D.
  TITLE     Genome wide analysis of acute myeloid leukemia reveal leukemia
            specific methylome and subtype specific hypomethylation of repeats
  JOURNAL   PLoS One 7 (3), e33213 (2012)
   PUBMED   22479372
  REMARK    GeneRIF: Results showed that DPP6, SPHKAP and ID4 were down
            regulated in acute myeloid leukemia (AML) patients.
REFERENCE   5  (bases 1 to 6954)
  AUTHORS   Means,C.K., Lygren,B., Langeberg,L.K., Jain,A., Dixon,R.E.,
            Vega,A.L., Gold,M.G., Petrosyan,S., Taylor,S.S., Murphy,A.N.,
            Ha,T., Santana,L.F., Tasken,K. and Scott,J.D.
  TITLE     An entirely specific type I A-kinase anchoring protein that can
            sequester two molecules of protein kinase A at mitochondria
  JOURNAL   Proc Natl Acad Sci U S A 108 (48), E1227-E1235 (2011)
   PUBMED   22084075
  REMARK    GeneRIF: SKIP is an entirely specific type I A-kinase anchoring
            protein that can sequester two molecules of protein kinase A at
            mitochondria.
REFERENCE   6  (bases 1 to 6954)
  AUTHORS   Anelli,V., Gault,C.R., Cheng,A.B. and Obeid,L.M.
  TITLE     Sphingosine kinase 1 is up-regulated during hypoxia in U87MG glioma
            cells. Role of hypoxia-inducible factors 1 and 2
  JOURNAL   J Biol Chem 283 (6), 3365-3375 (2008)
   PUBMED   18055454
  REMARK    GeneRIF: HIF-1 and HIF-2 have roles in SK1 upregulation during
            hypoxia in glioma cells
REFERENCE   7  (bases 1 to 6954)
  AUTHORS   You,B., Ren,A., Yan,G. and Sun,J.
  TITLE     Activation of sphingosine kinase-1 mediates inhibition of vascular
            smooth muscle cell apoptosis by hyperglycemia
  JOURNAL   Diabetes 56 (5), 1445-1453 (2007)
   PUBMED   17325258
  REMARK    GeneRIF: Hyperglycemia stimulates SK1 activity via protein kinase C
            and oxidative stress-dependent pathways, leading to decreased
            apoptosis in vvascular smooth muscle.
REFERENCE   8  (bases 1 to 6954)
  AUTHORS   Jeng,Y.J., Suarez,V.R., Izban,M.G., Wang,H.Q. and Soloff,M.S.
  TITLE     Progesterone-induced sphingosine kinase-1 expression in the rat
            uterus during pregnancy and signaling consequences
  JOURNAL   Am J Physiol Endocrinol Metab 292 (4), E1110-E1121 (2007)
   PUBMED   17164439
  REMARK    GeneRIF: Uterine expression of SphK1 mediates processes involved in
            growth and differentiation of uterine tissues during pregnancy.
REFERENCE   9  (bases 1 to 6954)
  AUTHORS   Yamanaka,M., Shegogue,D., Pei,H., Bu,S., Bielawska,A.,
            Bielawski,J., Pettus,B., Hannun,Y.A., Obeid,L. and Trojanowska,M.
  TITLE     Sphingosine kinase 1 (SPHK1) is induced by transforming growth
            factor-beta and mediates TIMP-1 up-regulation
  JOURNAL   J Biol Chem 279 (52), 53994-54001 (2004)
   PUBMED   15485866
  REMARK    GeneRIF: experiments demonstrate that sphingosine kinase
            1/sphingosine-1-phosphate are important components of the
            transforming growth factor-beta signaling pathway involved in
            up-regulation of the tissue inhibitor of metalloproteinase-1
            (TIMP-1)
REFERENCE   10 (bases 1 to 6954)
  AUTHORS   Lacana,E., Maceyka,M., Milstien,S. and Spiegel,S.
  TITLE     Cloning and characterization of a protein kinase A anchoring
            protein (AKAP)-related protein that interacts with and regulates
            sphingosine kinase 1 activity
  JOURNAL   J Biol Chem 277 (36), 32947-32953 (2002)
   PUBMED   12080051
  REMARK    GeneRIF: cloning and characterization; results indicate that SKIP
            is a novel protein likely to play a regulatory role in the
            modulation of sphingosine kinase type 1 (SPHK1) activity
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC109825.4, BC104956.1, AB051465.1 and AK095042.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001142644.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC104954.1, SRR1660805.163362.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392056.8/ ENSP00000375909.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                AC109825.4         136884-136925       c
            43-5221             BC104956.1         1-5179
            5222-6951           AB051465.1         3941-5670
            6952-6954           AK095042.1         3072-3074
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q36.3"
     gene            1..6954
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /note="SPHK1 interactor, AKAP domain containing"
                     /db_xref="GeneID:80309"
                     /db_xref="HGNC:HGNC:30619"
                     /db_xref="MIM:611646"
     exon            1..121
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..5192
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     A-kinase anchor protein SPHKAP; SPHK1 (sphingosine kinase
                     type 1) interacting protein; sphingosine kinase type
                     1-interacting protein; SPHK1-interactor and AKAP
                     domain-containing protein"
                     /codon_start=1
                     /product="A-kinase anchor protein SPHKAP isoform 1"
                     /protein_id="NP_001136116.1"
                     /db_xref="CCDS:CCDS46537.1"
                     /db_xref="GeneID:80309"
                     /db_xref="HGNC:HGNC:30619"
                     /db_xref="MIM:611646"
                     /translation="MDGNSLLSVPSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITAC
                     KKVLRSNSLLESTDYWLQNQRMPCQIGFVEDKSENCASVCFVNLDVNKDECSTEHLQQ
                     KLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQADFEVSQCPWLPDICLVQCA
                     RGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSIEEDFLTAS
                     EHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKY
                     PTPLIKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEK
                     SQALYIPKDAYFSMMDKDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYA
                     TNLAESVLQDAFIRLSQSQSTLPQESAVSVSVGSSLLPSCYSTKDTVVSRSWNELPKI
                     VVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSRQPQSALEVALACAATVIG
                     TISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCGMTQVAS
                     AVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAA
                     LVLTRPNTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDE
                     VHHKNMIIDPNDNRHSSEILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKE
                     TIRRRETEPSCQPSDPGASQAWTKATESSSSSPLSNSHNTSLVINNLVDGMYSKQDKG
                     GVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAGEDTKSPHHSENECRAS
                     SEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLLPAQS
                     TLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTP
                     NVPCRSLKRKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLED
                     VPDSVNLFANEVAAKIMNLTEFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPE
                     EDSEARAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFEL
                     LLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESITEEFYRYMLRDIER
                     DSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSLDG
                     FAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRK
                     NKIIVDDAEEADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTE
                     CKQPPVSSLSKTASLTNHSPLDSKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQR
                     EACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVRGGDTAVSACQIHSDSLDTRDVPEA
                     EASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLSERSMSNGNSSAT
                     SSLGIMDLDIYQESMPSSPMINELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLV
                     INFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKV
                     GDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG"
     exon            122..227
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            228..335
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            336..395
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            396..530
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            531..786
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            787..4537
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4538..4723
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4724..4810
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4811..4863
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4864..5048
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            5049..6954
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6935..6940
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /note="hexamer: AATAAA"
     polyA_site      6954
                     /gene="SPHKAP"
                     /gene_synonym="SKIP"
                     /note="major polyA site"
ORIGIN      
        1 agtctggcac tctgggagct gggtcctagc accacagact tatccttcgc ctgcactttc
       61 cgtctttctt ctctgggcgc ccaccaacaa tggatggcaa ctccctgctc tcggtaccaa
      121 gcaacttgga gtcatcacgg atgtatgacg ttttggaacc gcagcagggc agaggctgtg
      181 gcagctcagg aagcggcccg gggaactcca tcacagcctg taagaaggtt cttcgcagca
      241 atagcctgct ggagtcaaca gactactggt tgcagaatca gaggatgccc tgccaaattg
      301 gttttgtaga agacaagtct gaaaactgtg cttctgtctg ctttgtgaat cttgatgtga
      361 acaaggatga atgcagcaca gagcacctgc aacagaaact ggtcaacgtt tcaccagatc
      421 ttccaaaact tatcagttcc atgaatgtcc aacaaccaaa agaaaatgaa attgttgtcc
      481 taagtgggtt agcctctgga aatctccagg cagattttga agtttcacag tgcccttggc
      541 tgccagatat ctgcttggtc caatgtgcaa gagggaacag accaaacagt accaactgca
      601 tcatctttga aatcaacaaa tttctgattg gtctggaact ggtgcaggag cgacagctcc
      661 acctggaaac aaacatcttg aaactggagg atgacacgaa ctgttcctta tcttcaatcg
      721 aggaagactt tctcaccgct tctgagcact tggaggagga aagcgaggtg gatgaatcta
      781 ggaacgatta tgaaaatata aatgtctcag ccaatgtttt ggaaagtaaa cagctaaagg
      841 gagccaccca ggtggaatgg aattgcaaca aggaaaagtg gctttatgct ttggaagaca
      901 aatacatcaa caaatatccc acaccattga ttaaaacaga acgatctcca gaaaacctaa
      961 caaagaacac agccttgcag agtctagatc cctcagccaa gccatcacag tggaaaagag
     1021 aagctgtggg gaatgggaga caagccacac attattatca ttcagaagct tttaaaggtc
     1081 aaatggaaaa atcacaggca ctgtatattc caaaagatgc ttatttctcc atgatggata
     1141 aagatgtacc ttctgcatgt gctgtggcag agcagagaag caacctaaac ccaggagacc
     1201 atgaagacac aagaaacgct ctccctccta gacaagatgg agaagtcacc actggcaagt
     1261 atgctacaaa tttagcagaa tccgtgctgc aggatgcatt tattagatta tctcaatctc
     1321 agtccacatt accccaggaa tctgcagtca gtgtttctgt aggaagttct ctgcttccca
     1381 gttgctattc cacaaaagat acagtggttt ctcggtcatg gaatgagctc cccaaaatcg
     1441 tcgttgttca gagtccagat ggcagtgatg ctgccccaca gccaggcatc tcctcctggc
     1501 ctgagatgga agtctctgtt gaaacctcaa gcatcctctc tggagagaac tccagcagac
     1561 aaccccagag tgctctagaa gtggcgttag cttgtgcagc cactgtgatt ggaactattt
     1621 ccagtccaca ggccacagaa agactcaaaa tggagcaagt ggtctcgaac tttcccccag
     1681 ggagcagtgg tgcactgcaa actcaagcac cccaaggact caaggaacct tccatcaatg
     1741 agtactcctt tccatctgct ttgtgtggca tgactcaggt ggccagtgcc gtggctgtct
     1801 gtggtctggg tgaaagagaa gaggtgacat gctcagtggc tccaagtggt agcctcccgc
     1861 ctgcagctga ggcttctgaa gccatgcccc cactttgtgg tttagcaagc atggagcttg
     1921 gcaaggaagc cattgccaag ggattgctca aggaggctgc tctggtttta acaaggccta
     1981 atacctacag cagcattgga gactttctgg actccatgaa caggagaatc atggaaactg
     2041 cttcaaagtc tcagaccctg tgctcagaaa atgtcgtcag gaatgaactg gcacataccc
     2101 tgtccaatgt tatcctgagg cattccattg atgaagttca ccacaaaaat atgataatcg
     2161 accccaatga caacaggcat tcatctgaaa ttctggacac cttaatggaa agtacaaatc
     2221 aactgctttt agatgtgata tgcttcacgt tcaagaagat gagtcatatt gtacggcttg
     2281 gtgaatgtcc tgctgtcctt tctaaggaga ccatcagaag gagggagaca gaaccaagct
     2341 gccagccatc tgatccgggt gctagtcaag cttggacaaa agccactgaa tcctccagca
     2401 gctctccact tagcaattca cacaacacga gtcttgtcat caacaatctt gtggatggca
     2461 tgtattcaaa acaagacaag ggtggagtga ggccaggcct cttcaagaac cccacgctgc
     2521 agtcacaatt atcacgtagt cacagagtgc ccgattcttc aactgctaca acatcctcca
     2581 aggaaatata tctgaaagga atagcaggag aggatacaaa aagccctcat cacagtgaga
     2641 atgaatgcag agcctcttcc gaaggacaaa ggtccccaac ggtcagccag tccagaagtg
     2701 gttcccagga ggctgaggag agtatccacc caaacaccca agaaaagtac aactgtgcca
     2761 catctcgcat caacgaagtt caagtcaacc tgtccttgtt aggggatgac ctgctgcttc
     2821 ctgctcaatc cacgcttcaa acaaagcatc cagacatcta ctgcattaca gactttgcgg
     2881 aagaattagc agacacggtc gtctccatgg caactgaaat tgcagcgatt tgccttgaca
     2941 actccagtgg aaaacaaccc tggttttgtg catggaaaag agggagtgag tttctgatga
     3001 cacccaacgt accctgccga tccttgaaga ggaagaaaga gagccagggg agcgggaccg
     3061 ctgtgaggaa acacaagcct ccccggctca gtgagatcaa gaggaagacg gacgagcacc
     3121 ctgagcttaa agaaaagctg atgaacaggg ttgtggatga gtccatgaac cttgaagatg
     3181 tcccagattc tgtcaatctt tttgccaatg aagtggcagc caagatcatg aacctaacgg
     3241 agttctctat ggtggacggc atgtggcagg cgcagggcta tccccggaat cggttactga
     3301 gtggcgacag gtggagccgg ctgaaggcct ccagctgcga aagcattcct gaggaagact
     3361 ccgaggccag ggcctatgtc aacagcctgg gcttaatgag cacgctgagc cagccggtca
     3421 gcagggccag ctctgtctcc aagcagtcga gctgtgagag catcaccgat gagttttcca
     3481 ggttcatggt gaaccagatg gaaaatgaag ggagaggatt tgagttactg ctggattact
     3541 atgctggcaa gaacgccagc agcattctga actcagccat gcaacaggcg tgccggaaaa
     3601 gtgaccacct cagtgtgagg cctagctgtc cctctaagca gtccagcaca gagagcatca
     3661 ctgaggagtt ctacaggtac atgctgaggg acatcgaaag agacagcaga gaaagtgcct
     3721 cctccagacg gagcagccag gattggacag ccggcctgct gtctccttct ctgcgatccc
     3781 cagtgtgcca cagacagtcg tccatgccag acagcagatc cccatgctcc aggctgacag
     3841 tgaatgtgcc catcaaagcc aactctttag atggctttgc tcagaactgc ccacaagatt
     3901 tcctaagcgt gcagccggtc agtagcgcgt cctcatccgg tctctgcaaa tctgactctt
     3961 gcttgtatcg gagaggtggg actgaccaca tcaccaacat gttaattcat gaaacgtggg
     4021 ctagctccat tgaggctctc atgcgcaaga acaaaatcat tgtggatgat gcagaggaag
     4081 ctgacactga gcctgtttct ggtggctctc cctcgcaagc agagaagtgt gcaaatagat
     4141 tagctgcgag caggatgtgc agtgggccaa ctctgcttgt tcaggagtct ctcgattgcc
     4201 cgaggaaaga ctctgttacc gaatgtaaac agcccccagt gtcatctttg agcaaaactg
     4261 cttctcttac aaaccacagc cctttagatt ctaaaaaaga aacttcctcg tgccaggacc
     4321 ctgtaccaat aaaccacaaa aggcgatcac tttgctcgag ggaagtgcct ttgattcaga
     4381 ttgaaacaga tcagagagaa gcctgtgctg gggaacctga acccttcctt tccaaaagca
     4441 gcctcctaga ggaagcagaa gggcattcga atgacaaaaa catcccagat gtggtgagag
     4501 gtggagacac agccgtgagc gcttgtcaaa tccatagtga cagccttgat accagagatg
     4561 taccagaggc tgaagcctcc acagaagcca gagcccccga tgaggccccc aaccctccaa
     4621 gcagcagcga ggagagcaca ggcagctgga cccagcttgc caatgaggaa gacaacccag
     4681 atgacacaag tagctttctc cagctcagtg agcgatccat gagcaatggc aacagtagtg
     4741 ccactagcag tcttggcatt atggatctgg acatttatca ggaaagcatg ccatcttctc
     4801 ccatgattaa tgaattagta gaagaaaaga agattcttaa aggacagtca gaaagcacag
     4861 aggcacctgc atctggaccg cctacgggaa cagccagccc ccagaggagc ctgctggtga
     4921 tcaactttga cctggagcca gagtgtccag atgccgagct ccgagccact ctgcagtgga
     4981 tagctgcctc tgaactgggg attcccacca tctactttaa gaaatctcag gaaaacagaa
     5041 ttgaaaagtt tctagatgtc gtgcagctgg ttcatcggaa gtcctggaaa gtgggtgata
     5101 tcttccatgc agttgtccag tactgcaaaa tgcatgagga gcagaaggat gggagactga
     5161 gtctctttga ctggctcttg gaactgggat aataaggcag tctgccgtat agatcattcc
     5221 ttccctttat tccaacttag attacagtgg tttgttctaa atgctctaaa cattctcaaa
     5281 acatcacatc acattagcag aactataaaa aaaaatctgc tactcagatc cactgcatac
     5341 agaataagtc agaggaaaag caaaatatag gtctgtccaa attcatacaa cttgtgggtg
     5401 agttccaaag agcttggatt agaagggctg gacaaagaga gaattcaatg gggcccaaat
     5461 tagaatgctt ataatgagac ccaatctcca ggaaaacaac actcacataa gtttaatcat
     5521 ataaaatgat ttgtaatgtc tctaattaga tgaatcaact agaaacaaac tcagtggtca
     5581 aaataatttt taagagtatt ccgtaaccta tattttactt ttctgattat attaaggggc
     5641 tgccagcccg gagaaatact taagatatgg gtgagaaatc cccagacttt tatacaaaag
     5701 atttccactt tcaaatcaat gtcagtagac attgataaaa gtatagcagc atcctctact
     5761 gaggtgattt catttattcc ctgcagccca ctgataaata tctcacttct cccaaatagt
     5821 atgtggactc ccagctaagc agaaaactat tgtcattcaa ctgaagaaga ggaagataaa
     5881 agattgtctt gtttccatca ctgtattact tgtgtaacat gattacataa ttcttatcct
     5941 aagagaaagc tttcatattt aaaaaaaagt cttttcagat aaaatctgct tgtgtcttga
     6001 ataatatgaa atacaaactt tcactttatt ttattgtaaa ttataaagag attattgtct
     6061 taaataatat attgagttag cttcaagctt cctaaaatat gaagagattg ttgtctaaag
     6121 tcacatattg acattgagct cagtggcctg tttcatcacg tatgtgctgc tacctgtaca
     6181 gcagacatgc cgctccagtg acatttataa tgacagaagc agggtaatgg tcttgtgttt
     6241 gacatgatca gttaggatca tagactttcc ctgactcgta gatattagcc ttgaattggg
     6301 ggaaaagaag actttgacac attttagtta ttttaataac agagatttac tcttttgaaa
     6361 aataaaggta tctaatgtct ccctaataag tcttctttcc ttccaactaa atgacctaca
     6421 cggactttta ttttcttgat caaagaggtg tttattaagg acttctggat aactatactt
     6481 ttactctatt tttaaagatc acaaagtaat tttaaatgtg aacaggttcc cataccatga
     6541 atgctggcct caccttctct atcatccaca ttttgaaatg caaagaaagc tcccttgtaa
     6601 gccatacttc cttccccact cccatcctag gatacttgcc cagtgctcat taggcatttc
     6661 ttattcagat agtccaaatt taggttatta tgcttaattt gacacattaa ctaaatgccc
     6721 agttttaaaa tatatccatc aattcacgct gaaatgtgct tctttgtgct atcaaatgga
     6781 atagaataca cttatttttt aaacaatccc agaatactgt gtgtagactt ttgttgtgct
     6841 caaataaatg tttacttatc ttacaaagct caaatactgg attgtaacca tgtgatgaag
     6901 ttatctatgt tgtacctaac attgcaaatt aatcaataaa tctctgttgt caga
//
