LOCUS       NM_001142601            1816 bp    mRNA    linear   PRI 06-AUG-2024
DEFINITION  Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 3,
            mRNA.
ACCESSION   NM_001142601
VERSION     NM_001142601.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Piao,J., Su,Z., He,J., Zhu,T., Fan,F., Wang,X., Yang,Z., Zhan,H.
            and Luo,D.
  TITLE     SphK1 deficiency ameliorates the development of atherosclerosis by
            inhibiting the S1P/S1PR3/Rhoa/ROCK pathway
  JOURNAL   Cell Signal 121, 111252 (2024)
   PUBMED   38852936
  REMARK    GeneRIF: SphK1 deficiency ameliorates the development of
            atherosclerosis by inhibiting the S1P/S1PR3/Rhoa/ROCK pathway.
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Liao,K.H. and Liao,K.L.
  TITLE     Interference with sphingosine kinase-1 reduces the hydrogen
            peroxide-induced oxidative stress damage in melanocytes through
            four and a half LIM domains 2
  JOURNAL   Gen Physiol Biophys 43 (4), 321-333 (2024)
   PUBMED   38953574
  REMARK    GeneRIF: Interference with sphingosine kinase-1 reduces the
            hydrogen peroxide-induced oxidative stress damage in melanocytes
            through four and a half LIM domains 2.
REFERENCE   3  (bases 1 to 1816)
  AUTHORS   Liu,X.T., Huang,Y., Liu,D., Jiang,Y.C., Zhao,M., Chung,L.H.,
            Han,X.D., Zhao,Y., Chen,J., Coleman,P., Ting,K.K., Tran,C., Su,Y.,
            Dennis,C.V., Bhatnagar,A., Liu,K., Don,A.S., Vadas,M.A.,
            Gorrell,M.D., Zhang,S., Murray,M., Kavurma,M.M., McCaughan,G.W.,
            Gamble,J.R. and Qi,Y.
  TITLE     Targeting the SphK1/S1P/PFKFB3 axis suppresses hepatocellular
            carcinoma progression by disrupting glycolytic energy supply that
            drives tumor angiogenesis
  JOURNAL   J Transl Med 22 (1), 43 (2024)
   PUBMED   38200582
  REMARK    GeneRIF: Targeting the SphK1/S1P/PFKFB3 axis suppresses
            hepatocellular carcinoma progression by disrupting glycolytic
            energy supply that drives tumor angiogenesis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1816)
  AUTHORS   Alvarez,S.E., Harikumar,K.B., Hait,N.C., Allegood,J., Strub,G.M.,
            Kim,E.Y., Maceyka,M., Jiang,H., Luo,C., Kordula,T., Milstien,S. and
            Spiegel,S.
  TITLE     Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin
            ligase TRAF2
  JOURNAL   Nature 465 (7301), 1084-1088 (2010)
   PUBMED   20577214
REFERENCE   5  (bases 1 to 1816)
  AUTHORS   Pitson,S.M., Moretti,P.A., Zebol,J.R., Lynn,H.E., Xia,P.,
            Vadas,M.A. and Wattenberg,B.W.
  TITLE     Activation of sphingosine kinase 1 by ERK1/2-mediated
            phosphorylation
  JOURNAL   EMBO J 22 (20), 5491-5500 (2003)
   PUBMED   14532121
  REMARK    GeneRIF: Activation of this enzyme in mediated by ERK1/2
            phoshporylation
REFERENCE   6  (bases 1 to 1816)
  AUTHORS   Liu,H., Sugiura,M., Nava,V.E., Edsall,L.C., Kono,K., Poulton,S.,
            Milstien,S., Kohama,T. and Spiegel,S.
  TITLE     Molecular cloning and functional characterization of a novel
            mammalian sphingosine kinase type 2 isoform
  JOURNAL   J Biol Chem 275 (26), 19513-19520 (2000)
   PUBMED   10751414
REFERENCE   7  (bases 1 to 1816)
  AUTHORS   Melendez,A.J., Carlos-Dias,E., Gosink,M., Allen,J.M. and Takacs,L.
  TITLE     Human sphingosine kinase: molecular cloning, functional
            characterization and tissue distribution
  JOURNAL   Gene 251 (1), 19-26 (2000)
   PUBMED   10863092
REFERENCE   8  (bases 1 to 1816)
  AUTHORS   Nava,V.E., Lacana,E., Poulton,S., Liu,H., Sugiura,M., Kono,K.,
            Milstien,S., Kohama,T. and Spiegel,S.
  TITLE     Functional characterization of human sphingosine kinase-1
  JOURNAL   FEBS Lett 473 (1), 81-84 (2000)
   PUBMED   10802064
REFERENCE   9  (bases 1 to 1816)
  AUTHORS   Kohama,T., Olivera,A., Edsall,L., Nagiec,M.M., Dickson,R. and
            Spiegel,S.
  TITLE     Molecular cloning and functional characterization of murine
            sphingosine kinase
  JOURNAL   J Biol Chem 273 (37), 23722-23728 (1998)
   PUBMED   9726979
REFERENCE   10 (bases 1 to 1816)
  AUTHORS   Sudo,K., Chinen,K. and Nakamura,Y.
  TITLE     2058 expressed sequence tags (ESTs) from a human fetal lung cDNA
            library
  JOURNAL   Genomics 24 (2), 276-279 (1994)
   PUBMED   7698749
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK022402.1, BC030553.1 and
            DC384776.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001142601.1.
            
            Summary: The protein encoded by this gene catalyzes the
            phosphorylation of sphingosine to form sphingosine-1-phosphate
            (S1P), a lipid mediator with both intra- and extracellular
            functions. Intracellularly, S1P regulates proliferation and
            survival, and extracellularly, it is a ligand for cell surface G
            protein-coupled receptors. This protein, and its product S1P, play
            a key role in TNF-alpha signaling and the NF-kappa-B activation
            pathway important in inflammatory, antiapoptotic, and immune
            processes. Phosphorylation of this protein alters its catalytic
            activity and promotes its translocation to the plasma membrane.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Sep 2017].
            
            Transcript Variant: This variant (3) contains an alternate in-frame
            segment in the 5' coding region and uses a downstream start codon,
            compared to variant 2. Isoform 3 has a shorter N-terminus, compared
            to isoform 2. Variants 3, 4 and 5 encode the same isoform (3).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK022402.1, AK225250.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000592299.6/ ENSP00000465726.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                AK022402.1         1-41
            42-198              BC030553.1         1-157
            199-233             BC030553.1         521-555
            234-586             DC384776.1         207-559
            587-1816            BC030553.1         909-2138
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.1"
     gene            1..1816
                     /gene="SPHK1"
                     /gene_synonym="SPHK"
                     /note="sphingosine kinase 1"
                     /db_xref="GeneID:8877"
                     /db_xref="HGNC:HGNC:11240"
                     /db_xref="MIM:603730"
     exon            1..198
                     /gene="SPHK1"
                     /gene_synonym="SPHK"
                     /inference="alignment:Splign:2.1.0"
     exon            199..402
                     /gene="SPHK1"
                     /gene_synonym="SPHK"
                     /inference="alignment:Splign:2.1.0"
     CDS             393..1547
                     /gene="SPHK1"
                     /gene_synonym="SPHK"
                     /EC_number="2.7.1.91"
                     /note="isoform 3 is encoded by transcript variant 3; SK 1;
                     SPK 1; acetyltransferase SPHK1"
                     /codon_start=1
                     /product="sphingosine kinase 1 isoform 3"
                     /protein_id="NP_001136073.1"
                     /db_xref="CCDS:CCDS45785.1"
                     /db_xref="GeneID:8877"
                     /db_xref="HGNC:HGNC:11240"
                     /db_xref="MIM:603730"
                     /translation="MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAE
                     AEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQ
                     KPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASG
                     LRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRV
                     GSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAP
                     MGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDG
                     KGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL"
     exon            403..555
                     /gene="SPHK1"
                     /gene_synonym="SPHK"
                     /inference="alignment:Splign:2.1.0"
     exon            556..650
                     /gene="SPHK1"
                     /gene_synonym="SPHK"
                     /inference="alignment:Splign:2.1.0"
     exon            651..766
                     /gene="SPHK1"
                     /gene_synonym="SPHK"
                     /inference="alignment:Splign:2.1.0"
     exon            767..1816
                     /gene="SPHK1"
                     /gene_synonym="SPHK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1799..1804
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPHK1"
                     /gene_synonym="SPHK"
                     /note="hexamer: AATAAA"
     polyA_site      1816
                     /gene="SPHK1"
                     /gene_synonym="SPHK"
                     /note="major polyA site"
ORIGIN      
        1 agtgccctcc ccgctccgcg gcgccggctg cgaagttgag cgaaaagttt gaggccggag
       61 ggagcgaggc cggggagtcc gctccagcgg ggcgctccag tccctcagac gtgggctgag
      121 cttgggacga gctgcgttcc gccccaggcc actgtaggga acggcggtgg cgcctcccca
      181 gcaaaccgga ccgactgggt ccagccgccg cagggaatga cgccggtgct cctgcagcca
      241 cggctccggg cggggaaggc gagccccaca gccggccctg cgacgcccgc ctgggcagca
      301 ccgataagga gctgaaggca ggagccgccg ccacgggcag cgcccccaca gcgccaggga
      361 ccccctggca gcgggagccg cgggtcgagg ttatggatcc agcgggcggc ccccggggcg
      421 tgctcccgcg gccctgccgc gtgctggtgc tgctgaaccc gcgcggcggc aagggcaagg
      481 ccttgcagct cttccggagt cacgtgcagc cccttttggc tgaggctgaa atctccttca
      541 cgctgatgct cactgagcgg cggaaccacg cgcgggagct ggtgcggtcg gaggagctgg
      601 gccgctggga cgctctggtg gtcatgtctg gagacgggct gatgcacgag gtggtgaacg
      661 ggctcatgga gcggcctgac tgggagaccg ccatccagaa gcccctgtgt agcctcccag
      721 caggctctgg caacgcgctg gcagcttcct tgaaccatta tgctggctat gagcaggtca
      781 ccaatgaaga cctcctgacc aactgcacgc tattgctgtg ccgccggctg ctgtcaccca
      841 tgaacctgct gtctctgcac acggcttcgg ggctgcgcct cttctctgtg ctcagcctgg
      901 cctggggctt cattgctgat gtggacctag agagtgagaa gtatcggcgt ctgggggaga
      961 tgcgcttcac tctgggcacc ttcctgcgtc tggcagccct gcgcacctac cgcggccgac
     1021 tggcctacct ccctgtagga agagtgggtt ccaagacacc tgcctccccc gttgtggtcc
     1081 agcagggccc ggtagatgca caccttgtgc cactggagga gccagtgccc tctcactgga
     1141 cagtggtgcc cgacgaggac tttgtgctag tcctggcact gctgcactcg cacctgggca
     1201 gtgagatgtt tgctgcaccc atgggccgct gtgcagctgg cgtcatgcat ctgttctacg
     1261 tgcgggcggg agtgtctcgt gccatgctgc tgcgcctctt cctggccatg gagaagggca
     1321 ggcatatgga gtatgaatgc ccctacttgg tatatgtgcc cgtggtcgcc ttccgcttgg
     1381 agcccaagga tgggaaaggt gtgtttgcag tggatgggga attgatggtt agcgaggccg
     1441 tgcagggcca ggtgcaccca aactacttct ggatggtcag cggttgcgtg gagcccccgc
     1501 ccagctggaa gccccagcag atgccaccgc cagaagagcc cttatgaccc ctgggccgcg
     1561 ctgtgcctta gtgtctactt gcaggaccct tcctccttcc ctagggctgc agggcctgtc
     1621 cacagctcct gtgggggtgg aggagactcc tctggagaag ggtgagaagg tggaggctat
     1681 gctttggggg gacaggccag aatgaagtcc tgggtcagga gcccagctgg ctgggcccag
     1741 ctgcctatgt aaggccttct agtttgttct gagaccccca ccccacgaac caaatccaaa
     1801 taaagtgaca ttccca
//
