LOCUS       NM_025137               7772 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens SPG11 vesicle trafficking associated, spatacsin
            (SPG11), transcript variant 1, mRNA.
ACCESSION   NM_025137
VERSION     NM_025137.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Pierga,A., Matusiak,R., Cauhape,M., Branchu,J., Danglot,L.,
            Boutry,M. and Darios,F.
  TITLE     Spatacsin regulates directionality of lysosome trafficking by
            promoting the degradation of its partner AP5Z1
  JOURNAL   PLoS Biol 21 (10), e3002337 (2023)
   PUBMED   37871017
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Regensburger,M., Krumm,L., Schmidt,M.A., Schmid,A., Spatz,I.T.,
            Marterstock,D.C., Kopp,C., Kohl,Z., Doerfler,A., Karrasch,T.,
            Winner,B. and Winkler,J.
  TITLE     Neurometabolic Dysfunction in SPG11 Hereditary Spastic Paraplegia
  JOURNAL   Nutrients 14 (22), 4803 (2022)
   PUBMED   36432490
  REMARK    GeneRIF: Neurometabolic Dysfunction in SPG11 Hereditary Spastic
            Paraplegia.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7772)
  AUTHORS   Cogo,S., Tomkins,J.E., Vavouraki,N., Giusti,V., Forcellato,F.,
            Franchin,C., Tessari,I., Arrigoni,G., Cendron,L., Manzoni,C.,
            Civiero,L., Lewis,P.A. and Greggio,E.
  TITLE     Cytosolic sequestration of spatacsin by Protein Kinase A and 14-3-3
            proteins
  JOURNAL   Neurobiol Dis 174, 105858 (2022)
   PUBMED   36096339
REFERENCE   4  (bases 1 to 7772)
  AUTHORS   Rattay,T.W., Schols,L., Zeltner,L., Rohrschneider,W.K., Ernemann,U.
            and Lindig,T.
  TITLE     'Ears of the lynx' sign and thin corpus callosum on MRI in
            heterozygous SPG11 mutation carriers
  JOURNAL   J Neurol 269 (11), 6148-6151 (2022)
   PUBMED   35614164
  REMARK    GeneRIF: ''Ears of the lynx'' sign and thin corpus callosum on MRI
            in heterozygous SPG11 mutation carriers.
REFERENCE   5  (bases 1 to 7772)
  AUTHORS   Martinez Murillo,F., Kobayashi,H., Pegoraro,E., Galluzzi,G.,
            Creel,G., Mariani,C., Farina,E., Ricci,E., Alfonso,G., Pauli,R.M.
            and Hoffman,E.P.
  TITLE     Genetic localization of a new locus for recessive familial spastic
            paraparesis to 15q13-15
  JOURNAL   Neurology 53 (1), 50-56 (1999)
   PUBMED   10408536
REFERENCE   6  (bases 1 to 7772)
  AUTHORS   Hentati,A., Ouahchi,K., Pericak-Vance,M.A., Nijhawan,D., Ahmad,A.,
            Yang,Y., Rimmler,J., Hung,W., Schlotter,B., Ahmed,A., Ben
            Hamida,M., Hentati,F. and Siddique,T.
  TITLE     Linkage of a commoner form of recessive amyotrophic lateral
            sclerosis to chromosome 15q15-q22 markers
  JOURNAL   Neurogenetics 2 (1), 55-60 (1998)
   PUBMED   9933301
REFERENCE   7  (bases 1 to 7772)
  AUTHORS   Almenoff,J.S., Jurka,J. and Schoolnik,G.K.
  TITLE     Induction of heat-stable enterotoxin receptor activity by a human
            Alu repeat
  JOURNAL   J Biol Chem 269 (24), 16610-16617 (1994)
   PUBMED   8206979
REFERENCE   8  (bases 1 to 7772)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
REFERENCE   9  (bases 1 to 7772)
  AUTHORS   Siddique,N. and Siddique,T.
  TITLE     Amyotrophic Lateral Sclerosis Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301623
REFERENCE   10 (bases 1 to 7772)
  AUTHORS   Stevanin,G.
  TITLE     Spastic Paraplegia 11
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301389
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009996.7, AB058743.2 and
            BC094704.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_025137.3.
            
            Summary: The protein encoded by this gene is a potential
            transmembrane protein that is phosphorylated upon DNA damage.
            Defects in this gene are a cause of spastic paraplegia type 11
            (SPG11). Multiple transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, May 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC153879.1, AB058743.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261866.12/ ENSP00000261866.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AC009996.7         149475-149488       c
            15-1402             AB058743.2         1-1388
            1403-1403           AC009996.7         137385-137385       c
            1404-7766           AB058743.2         1390-7752
            7767-7772           BC094704.1         2277-2282
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.1"
     gene            1..7772
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /note="SPG11 vesicle trafficking associated, spatacsin"
                     /db_xref="GeneID:80208"
                     /db_xref="HGNC:HGNC:11226"
                     /db_xref="MIM:610844"
     exon            1..272
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..7347
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /note="isoform 1 is encoded by transcript variant 1;
                     spatacsin; spastic paraplegia 11 protein; colorectal
                     carcinoma-associated protein; SPG11, spatacsin vesicle
                     trafficking associated; spastic paraplegia 11 (autosomal
                     recessive); amyotrophic lateral sclerosis 5"
                     /codon_start=1
                     /product="spatacsin isoform 1"
                     /protein_id="NP_079413.3"
                     /db_xref="CCDS:CCDS10112.1"
                     /db_xref="GeneID:80208"
                     /db_xref="HGNC:HGNC:11226"
                     /db_xref="MIM:610844"
                     /translation="MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSR
                     AQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKP
                     KLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFH
                     NNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSS
                     LGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSS
                     SNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQI
                     DRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIM
                     HGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTI
                     TLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIH
                     GSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSD
                     QFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKE
                     LFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNI
                     WKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKE
                     ASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEK
                     LYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALW
                     WDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQ
                     NKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQ
                     DTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEA
                     HPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATT
                     MYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITI
                     YHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLH
                     NGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLG
                     LDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLE
                     EGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHS
                     QLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQ
                     EMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSV
                     EDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLV
                     MEMYELCMFFRNYKEAEAKLLEFQKSLETLNTAATKVHPVIPAMWLEDQVCFLLKLML
                     QQCKTQYELGKLLQLFVEREHLFSDGPDVKKLCILCQILKDTSIAINHTIITSYSIEN
                     LQHECRSILERLQTDGQFALARRVAELAELPVDNLVIKEITQEMQTLKHIEQWSLKQA
                     RIDFWKKCHENFKKNSISSKAASSFFSTQAHVACEHPTGWSSMEERHLLLTLAGHWLA
                     QEDVVPLDKLEELEKQIWLCRITQHTLGRNQEETEPRFSRQISTSGELSFDSLASEFS
                     FSKLAALNTSKYLELNSLPSKETCENRLDWKEQESLNFLIGRLLDDGCVHEASRVCRY
                     FHFYNPDVALVLHCRALASGEASMEDLHPEIHALLQSAELLEEEAPDIPLRRVHSTSS
                     LDSQKFVTVPSSNEVVTNLEVLTSKCLHGKNYCRQVLCLYDLAKELGCSYTDVAAQDG
                     EAMLRKILASQQPDRCKRAQAFISTQGLKPDTVAELVAEEVTRELLTSSQGTGHKQMF
                     NPTEESQTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHM
                     EGIIRVLQAAHMLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMR
                     KKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIES
                     QPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQAQHCQRLTKLITLQIHFLN
                     TGQNTMLINLGRHKLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYL
                     EEFKQQRLLKSSIFEEISKKYKQHQPTDMVMENLKKLLTYCEDVYLYYKLAYEHKFYE
                     IVNVLLKDPQTGCCLKDMLAG"
     exon            273..457
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            458..682
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            683..884
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1022
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1471
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1617
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1750
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1906
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..2082
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            2083..2259
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            2260..2331
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2459
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2635
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            2636..2849
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            2850..3053
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            3054..3160
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            3161..3306
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            3307..3468
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            3469..3535
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            3536..3701
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            3702..3907
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            3908..4016
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            4017..4176
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            4177..4449
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            4450..4650
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            4651..4758
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            4759..4921
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            4922..5136
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            5137..5881
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            5882..6021
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            6022..6220
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            6221..6358
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            6359..6492
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            6493..6600
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            6601..6769
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            6770..6858
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            6859..7014
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            7015..7166
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     exon            7167..7772
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7751..7756
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /note="hexamer: AATAAA"
     polyA_site      7772
                     /gene="SPG11"
                     /gene_synonym="ALS5; CMT2X; KIAA1840"
                     /note="major polyA site"
ORIGIN      
        1 aaccgccggg ccaagatggc tgcagaggaa ggggtcgcga gtgctgcttc cgccggcggt
       61 agctggggca ccgcggccat ggggcgggtt ctaccgatgc tgttggtgcc agtccccgcc
      121 gaggcgatgg ggcagctcgg ctcccgggcg cagctgcgca cacagccgga ggctctgggg
      181 agcctgacgg ctgcgggcag cctccaagtg ctttctttga cgcctggcag ccggggcggg
      241 ggtcgctgct gcctggaggg ccccttctgg cactttctat gggaggattc tcgtaacagc
      301 agcacaccaa ctgaaaagcc caaactgctc gctcttggtg aaaattatga actgcttatc
      361 tatgaattta atttgaaaga tggaagatgt gatgcaacca ttttgtatag ctgtagtagg
      421 gaggcattgc aaaagctcat tgacgatcaa gatatcagta tttccttatt gtctttgaga
      481 atcctgtcat ttcacaataa cacatcatta ctgttcatca acaaatgtgt catcctacat
      541 attatatttc ctgaaagaga tgctgcaatt agagtactca actgtttcac acttcccttg
      601 cctgcacagg cagtggacat gattattgac acgcagctct gcagaggaat tctttttgtt
      661 ttgagtagtt taggctggat ctacattttt gatgttgtgg atggtacata tgtagctcat
      721 gtggatttag cacttcacaa agaagacatg tgtaatgagc agcaacagga gccagccaag
      781 atttcttcat ttacttcact gaaagtttct caagacctcg atgttgcagt gattgtcagc
      841 tcctccaact ccgcagttgc tcttaactta aatttgtatt tcaggcaaca cccaggacac
      901 ctactgtgtg aaagaatact agaagatctt cctattcaag gacctaaggg cgtagatgaa
      961 gatgatcctg ttaactctgc ctacaacatg aaactggcca agttttcctt ccaaattgat
     1021 aggtcttgga aagcccagct atcatcattg aatgaaacaa taaagaactc caaactggag
     1081 gtttcctgtt gtgctccatg gttccaggat attttgcatt tggagtcacc tgaatctggt
     1141 aaccacagta caagtgtgca gagctgggcc ttcattccac aggacataat gcatgggcaa
     1201 tataatgttc tacagaaaga tcatgccaag accagtgatc caggaagatc atggaaaata
     1261 atgcacatca gtgaacaaga ggaacccata gagcttaaat gtgtgtctgt gacaggattc
     1321 actgcactgt ttacttggga agtggaaagg atgggctata ccattaccct ctgggatttg
     1381 gagacccagg gcatgcagtg tttttccctt ggcacaaagt gtattcctgt agacagtagt
     1441 ggagaccagc agctgtgctt tgttttgaca gagaatggac tctctctgat tttgtttggt
     1501 ttgactcaag aagagttttt aaacagactc atgatccatg gaagtgccag cactgtggac
     1561 actctttgtc atctcaatgg ctggggaagg tgctcaattc ccatacatgc actagaggcc
     1621 gggatagaaa atcgtcagct ggacacagta aatttctttt tgaagagcaa ggaaaatctt
     1681 tttaatccat cctcaaaatc ttctgtatct gatcagtttg atcacttgtc atcccattta
     1741 tatttaagaa atgtggaaga gctgatacca gcattggatt tactttgctc ggcaattaga
     1801 gaaagttatt ctgaacccca aagcaaacac ttttcagaac aattgcttaa tcttacactg
     1861 tctttcctta acaaccaaat aaaggagctt ttcattcaca ctgaagaact agatgaacat
     1921 ctgcaaaaag gagtgaacat tttgactagc tacattaatg aacttcgaac cttcatgata
     1981 aagtttcctt ggaagctaac agatgctata gatgaatatg atgtacatga aaatgtcccc
     2041 aaagtaaagg agagcaatat atggaagaaa ctcagctttg aggaagttat tgccagcgcc
     2101 attttaaaca acaaaatacc agaggcacag actttcttca ggattgatag tcattctgct
     2161 caaaaacttg aggagcttat tggcataggc ctaaatttgg tctttgacaa tttaaaaaag
     2221 aacaatataa aggaagcctc tgaacttttg aagaatatgg ggtttgatgt aaaaggccaa
     2281 ttgctcaaga tctgcttcta tacaactaat aaaaatatac gtgacttttt ggttgaaatt
     2341 ttaaaagaaa aaaattattt ttctgaaaaa gagaaaagaa ctatagactt cgtgcatcaa
     2401 gttgagaagc tttatttggg acatttccaa gaaaatatgc aaatccagtc atttcccagg
     2461 tactggataa aggaacaaga ttttttcaag cacaagtctg ttttggactc attcctgaaa
     2521 tatgattgta aagatgaatt taacaaacag gaccatagaa ttgtgttaaa ttgggctctg
     2581 tggtgggatc aactaacaca agaatccatc cttctcccca ggataagtcc agaagaatac
     2641 aaatcatatt cccctgaagc cctctggaga tacctcacag ctcgccatga ttggttaaac
     2701 attatcttat ggattggaga atttcaaacc cagcatagtt atgcttcact tcagcagaac
     2761 aaatggcccc ttctgactgt tgatgttatt aaccagaata cttcctgtaa caactacatg
     2821 aggaatgaaa ttttagataa gctggccagg aatggggttt ttttggcatc tgaactggaa
     2881 gactttgaat gcttcctcct aagactgagc cgtattggag gtgtaataca ggataccctc
     2941 cctgttcaaa actacaagac caaagaaggt tgggatttcc attctcaatt cattctctat
     3001 tgtttggagc acagtctgca gcatcttctt tatgtctacc ttgactgtta caaacttagt
     3061 cctgaaaatt gtcccttttt ggaaaaaaaa gagttacatg aagcacaccc ttggtttgaa
     3121 tttttagttc agtgtcgaca agttgccagt aacttaacag atcccaaact gatcttccag
     3181 gctagccttg caaatgctca gattttgatt cccaccaatc aggccagtgt aagcagtatg
     3241 ctattggaag gacataccct cctggccctt gctactacaa tgtattctcc tgggggtgtc
     3301 agtcaggttg ttcagaatga agaaaatgaa aactgtttga agaaagtgga tccccagcta
     3361 ttgaagatgg cattaactcc ttaccccaag ctaaaaactg ctctcttccc acagtgcact
     3421 cctcctagtg tcctgccatc tgatattaca atctaccacc ttattcagtc attatcaccc
     3481 tttgatccta gcagattgtt tggctggcag tctgctaaca cactagctat aggagatgca
     3541 tggagtcatc tcccacattt ctctagccct gacctggtta ataaatatgc tatagtggaa
     3601 cgtctgaatt ttgcttatta tttacataat gggcggccat catttgcatt tggtactttt
     3661 ctggtccagg aattaatcaa gagcaagact cccaagcagc tgatccagca agtaggcaat
     3721 gaagcctatg ttatagggct ctcctccttc cacatacctt caataggagc tgcatgtgtt
     3781 tgtttcttag aattgcttgg ccttgacagc ctcaagctca gagttgatat gaaagtggcc
     3841 aatataattt tgagctacaa gtgcagaaat gaagatgctc agtacagctt tatcagagag
     3901 tctgtagccg aaaaactatc taaactagct gatggtgaaa agacaaccac agaagaattg
     3961 cttgttctct tagaagaagg tacatggaac agcattcagc aacaggaaat aaagaggtta
     4021 tccagtgaat ctagcagcca atgggcatta gtggtgcagt tctgcaggct acacaatatg
     4081 aaactaagca tatcttacct tagagaatgt gccaaagcaa atgattggct gcagttcatt
     4141 attcacagcc aactccacaa ctaccaccca gcagaggtga aatcccttat ccagtacttc
     4201 agcccagtca ttcaagacca cttaaggctg gcttttgaga acttgccctc agtgcccacc
     4261 tccaaaatgg acagcgatca agtctgcaat aagtgccccc aggaacttca aggaagcaaa
     4321 caagagatga ccgatttatt tgaaattctg ctccaatgct cagaggagcc agactcctgg
     4381 cactggcttc tggttgaagc agtgaaacaa caggccccta tcctcagtgt tctggcctca
     4441 tgtctccagg gtgccagtgc catttcttgt ctctgtgttt ggatcatcac ttctgtggag
     4501 gacaatgttg caactgaagc aatgggacac attcaggact caacagagga ccatacctgg
     4561 aaccttgagg atctttcagt catctggaga acattattaa caagacaaaa gagcaaaact
     4621 ctcatcagag gtttccagct tttctttaag gattccccgt tactactggt gatggagatg
     4681 tatgaactgt gtatgttctt caggaattat aaagaagctg aagctaaact tctggagttt
     4741 cagaagagcc ttgaaacgct taacacagca gccacaaagg tccaccctgt catccctgcc
     4801 atgtggctgg aggatcaggt gtgtttcctt ttgaagctta tgctacagca gtgtaagacc
     4861 cagtatgagc tggggaagct tttacagctc tttgttgaaa gagagcatct cttctctgat
     4921 ggtccagatg tgaaaaagct ttgcatcctt tgccagattt tgaaggatac atccatagcc
     4981 attaatcata caattattac cagctacagc attgagaatc ttcagcatga atgtagatct
     5041 attttggaaa gactgcagac agatggacaa ttcgctttgg ccaggagggt agcagaatta
     5101 gctgagttac ctgtggacaa cttggttatt aaagagataa cacaggaaat gcagacccta
     5161 aaacacattg aacagtggtc actaaaacaa gcaagaattg acttctggaa aaaatgccat
     5221 gagaatttta agaaaaattc aatttcaagc aaagcagctt cttccttttt ctcaacccag
     5281 gcccatgtgg catgtgagca cccaactgga tggagcagca tggaggagcg ccatctgctg
     5341 ctcaccttgg cagggcactg gcttgcccag gaggacgtgg tgcccttgga taagctggag
     5401 gagctggaga agcagatctg gctgtgccgc atcacccagc acactcttgg aagaaatcag
     5461 gaggaaacag agcccagatt ttctcgacag atctcaacta gtggtgaact ttcctttgat
     5521 agtttagcca gtgagttttc cttctccaag ttggctgctc tgaacacatc aaaatactta
     5581 gaacttaaca gccttccatc caaagagaca tgcgagaata gattggattg gaaagagcag
     5641 gagtcactaa actttttgat tgggcgccta ctggatgatg gctgtgtgca tgaagcaagt
     5701 agagtatgcc ggtattttca tttttataat ccagatgtcg ccttggtatt gcactgcaga
     5761 gcactggcct caggggaagc tagtatggag gatctgcacc cagagatcca tgctctccta
     5821 caaagtgctg agctgcttga ggaagaagca cccgacattc ccctaaggag agtccacagc
     5881 acttcaagtc tggatagtca gaagtttgtg acagtgccct ccagtaatga agtggtaact
     5941 aacctggaag tgctgacaag caaatgcctc catgggaaga actactgtcg acaggtcctc
     6001 tgtctgtatg atcttgccaa ggagttgggc tgttcctaca cagatgttgc tgctcaggat
     6061 ggtgaagcca tgctccggaa aatcttggcc tctcagcagc ctgaccgatg caaacgagcc
     6121 caggccttca tcagcacaca gggccttaag ccagatactg tggctgaact cgtggcagaa
     6181 gaggtgacac gggagctgct tacttcatca cagggaacag gacataagca gatgttcaac
     6241 ccaacagagg aaagccagac atttcttcag ctgaccactc tgtgtcaaga ccgcacattg
     6301 gtaggcatga agttgttgga taagatttcc tccgttcccc atggggaact gtcttgcacc
     6361 acagagctcc tgatcctggc ccatcattgc ttcaccctga cgtgccacat ggagggcatc
     6421 atccgagtcc tacaggccgc ccacatgctc acagataacc acctggcccc cagtgaggag
     6481 tatgggctgg tggtacggct cctcactggc attggaaggt acaacgagat gacatacata
     6541 tttgatttgc tgcataaaaa gcactacttt gaagtgctaa tgaggaagaa gttggatccg
     6601 agtggtaccc tgaaaacagc cctgctggac tacatcaaac gctgccgtcc tggagacagt
     6661 gaaaagcaca atatgattgc cctgtgcttc agcatgtgcc gggagattgg cgagaaccac
     6721 gaggcagctg cccgcatcca actgaaattg attgagtctc agccctggga ggacagcctc
     6781 aaggatgggc accagctgaa acaactgctg ctgaaggccc tgactctgat gttggatgca
     6841 gcagagagtt atgccaagga ctcctgtgtg cgacaggccc agcactgtca gcggctcacc
     6901 aagttgataa ctctgcagat tcactttctg aacactggcc agaacacaat gctcatcaac
     6961 ttgggccgcc acaagctgat ggactgtatt ctggccctac ctcggttcta ccaggcttct
     7021 attgtggctg aggcctacga ttttgttcca gattgggctg aaattttata ccagcaagtg
     7081 attcttaaag gagactttaa ttacttggaa gaatttaagc agcaaaggtt attaaagtcc
     7141 agtatatttg aagagatttc caaaaaatat aaacaacatc agcctactga catggtcatg
     7201 gaaaacctga agaaattact cacatattgt gaagatgttt acctgtatta caagttggca
     7261 tacgaacaca agttttatga aattgtaaat gtgcttctga aggaccctca gacaggttgc
     7321 tgtctaaagg acatgctagc aggttagatg atttcatagg tgtctgtttt cttgtactgt
     7381 tagcagattc tgacagatgt gatgagaaga agaatgcatt ggagatcttt gctaaagttg
     7441 aacaatcccg gtactgtacc atatcagtcc tttgtgggta gtaggtagca agtaagaaac
     7501 ttttcaggag gaaattccta tttaaaatag attgatttta gatgattgtt catccacacc
     7561 attttatata gatactagta ttaagatcaa aagcttcctc ttcctcagga cagcttctac
     7621 tttagatgat ccaataatga ttaaagaata cctgtacctg cagattccag tttcaaagaa
     7681 atttaattat tatttacaca gttaaggaac aggtgataca ttttcatttg ttagaaactg
     7741 atctttctgt aataaaatag attttcaatt ca
//
