LOCUS       NM_024867               5662 bp    mRNA    linear   PRI 05-AUG-2024
DEFINITION  Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA.
ACCESSION   NM_024867
VERSION     NM_024867.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Lu,W., Li,Y., Meng,L., Tan,C., Nie,H., Zhang,Q., Song,Y., Zhang,H.,
            Tan,Y.Q., Tu,C., Guo,H., Wu,L. and Du,J.
  TITLE     Novel SPEF2 variants cause male infertility and likely primary
            ciliary dyskinesia
  JOURNAL   J Assist Reprod Genet 41 (6), 1485-1498 (2024)
   PUBMED   38568462
  REMARK    GeneRIF: Novel SPEF2 variants cause male infertility and likely
            primary ciliary dyskinesia.
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Li,D.Y., Yang,X.X., Tu,C.F., Wang,W.L., Meng,L.L., Lu,G.X.,
            Tan,Y.Q., Zhang,Q.J. and Du,J.
  TITLE     Sperm flagellar 2 (SPEF2) is essential for sperm flagellar assembly
            in humans
  JOURNAL   Asian J Androl 24 (4), 359-366 (2022)
   PUBMED   34755699
  REMARK    GeneRIF: Sperm flagellar 2 (SPEF2) is essential for sperm flagellar
            assembly in humans.
REFERENCE   3  (bases 1 to 5662)
  AUTHORS   Cindric,S., Dougherty,G.W., Olbrich,H., Hjeij,R., Loges,N.T.,
            Amirav,I., Philipsen,M.C., Marthin,J.K., Nielsen,K.G.,
            Sutharsan,S., Raidt,J., Werner,C., Pennekamp,P., Dworniczak,B. and
            Omran,H.
  TITLE     SPEF2- and HYDIN-Mutant Cilia Lack the Central Pair-associated
            Protein SPEF2, Aiding Primary Ciliary Dyskinesia Diagnostics
  JOURNAL   Am J Respir Cell Mol Biol 62 (3), 382-396 (2020)
   PUBMED   31545650
  REMARK    GeneRIF: In individuals with primary ciliary dyskinesia and central
            pair defects, the CP-associated protein SPEF2 is absent in
            HYDIN-mutant cells. 41 of 189 individuals had undetectable SPEF2
            and were subjected to a genetic analysis, which revealed one novel
            loss-of-function mutation in SPEF2 and 3 reported and 13 novel
            HYDIN mutations in 15 individuals. A mutation of SPEF2 is causative
            for PCD with a CP defect.
REFERENCE   4  (bases 1 to 5662)
  AUTHORS   Tu,C., Nie,H., Meng,L., Wang,W., Li,H., Yuan,S., Cheng,D., He,W.,
            Liu,G., Du,J., Gong,F., Lu,G., Lin,G., Zhang,Q. and Tan,Y.Q.
  TITLE     Novel mutations in SPEF2 causing different defects between flagella
            and cilia bridge: the phenotypic link between MMAF and PCD
  JOURNAL   Hum Genet 139 (2), 257-271 (2020)
   PUBMED   31942643
  REMARK    GeneRIF: Mutations in SPEF2 are associated with multiple
            morphological abnormalities of the sperm flagella and primary
            ciliary dyskinesia.
REFERENCE   5  (bases 1 to 5662)
  AUTHORS   Sha,Y., Liu,W., Wei,X., Zhu,X., Luo,X., Liang,L. and Guo,T.
  TITLE     Biallelic mutations in Sperm flagellum 2 cause human multiple
            morphological abnormalities of the sperm flagella (MMAF) phenotype
  JOURNAL   Clin Genet 96 (5), 385-393 (2019)
   PUBMED   31278745
  REMARK    GeneRIF: Biallelic mutations in Sperm flagellum 2 cause human
            multiple morphological abnormalities of the sperm flagella (MMAF)
            phenotype.
REFERENCE   6  (bases 1 to 5662)
  AUTHORS   Wang,J.H., Pappas,D., De Jager,P.L., Pelletier,D., de Bakker,P.I.,
            Kappos,L., Polman,C.H., Chibnik,L.B., Hafler,D.A., Matthews,P.M.,
            Hauser,S.L., Baranzini,S.E. and Oksenberg,J.R.
  CONSRTM   Australian and New Zealand Multiple Sclerosis Genetics Consortium
            (ANZgene)
  TITLE     Modeling the cumulative genetic risk for multiple sclerosis from
            genome-wide association data
  JOURNAL   Genome Med 3 (1), 3 (2011)
   PUBMED   21244703
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5662)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 5662)
  AUTHORS   Sironen,A., Vilkki,J., Bendixen,C. and Thomsen,B.
  TITLE     Infertile Finnish Yorkshire boars carry a full-length LINE-1
            retrotransposon within the KPL2 gene
  JOURNAL   Mol Genet Genomics 278 (4), 385-391 (2007)
   PUBMED   17610085
REFERENCE   9  (bases 1 to 5662)
  AUTHORS   Sironen,A., Thomsen,B., Andersson,M., Ahola,V. and Vilkki,J.
  TITLE     An intronic insertion in KPL2 results in aberrant splicing and
            causes the immotile short-tail sperm defect in the pig
  JOURNAL   Proc Natl Acad Sci U S A 103 (13), 5006-5011 (2006)
   PUBMED   16549801
REFERENCE   10 (bases 1 to 5662)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC025448.6, BC067761.1, BC016773.1, BC110984.1, AL832607.1,
            AK097547.1, AB051557.1, DA295300.1 and BX648691.1.
            
            On Nov 22, 2018 this sequence version replaced NM_024867.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM005670.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356031.8/ ENSP00000348314.3
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC025448.6         93010-93033
            25-637              BC067761.1         44-656
            638-1011            BC016773.1         618-991
            1012-1632           BC110984.1         903-1523
            1633-1659           BC067761.1         1653-1679
            1660-2276           AL832607.1         544-1160
            2277-2783           AK097547.1         994-1500
            2784-2845           AB051557.1         1101-1162
            2846-2974           DA295300.1         442-570
            2975-5514           AB051557.1         1292-3831
            5515-5662           BX648691.1         2999-3146
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.2"
     gene            1..5662
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /note="sperm flagellar 2"
                     /db_xref="GeneID:79925"
                     /db_xref="HGNC:HGNC:26293"
                     /db_xref="MIM:610172"
     exon            1..193
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..5604
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cancer/testis antigen 122; sperm flagellar protein 2;
                     testis tissue sperm-binding protein Li 47a"
                     /codon_start=1
                     /product="sperm flagellar protein 2 isoform 1"
                     /protein_id="NP_079143.3"
                     /db_xref="CCDS:CCDS43309.1"
                     /db_xref="GeneID:79925"
                     /db_xref="HGNC:HGNC:26293"
                     /db_xref="MIM:610172"
                     /translation="MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQ
                     DDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYI
                     ALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQ
                     EKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIP
                     KPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQT
                     TTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQL
                     INRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALA
                     KQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLT
                     NNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYK
                     NMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLG
                     KTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPV
                     LQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTT
                     RAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEA
                     LTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDI
                     IAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPEN
                     ILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTP
                     PPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKG
                     SPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPY
                     WELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADF
                     NSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGM
                     TMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSES
                     QLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASL
                     AVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSP
                     PMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALL
                     EDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFF
                     INGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDIL
                     IDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEE
                     ELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIE
                     FFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSA
                     EKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLK
                     VFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLI
                     SNYSDYKFPDIKIILQRSEHVQGSDGERSPSRHTEEKK"
     exon            194..296
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            297..549
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            550..720
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            721..861
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            862..926
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1113
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1302
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1490
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1659
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            1660..1879
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2034
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2110
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2172
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            2173..2276
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2533
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..2642
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..2800
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2974
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            2975..3049
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            3050..3198
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..3326
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            3327..3465
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            3466..3603
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            3604..3755
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            3756..3936
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            3937..4084
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            4085..4213
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            4214..4352
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            4353..4582
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            4583..4689
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            4690..4872
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            4873..4965
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            4966..5145
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            5146..5391
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            5392..5514
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     exon            5515..5662
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5636..5641
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /note="hexamer: AATAAA"
     polyA_site      5662
                     /gene="SPEF2"
                     /gene_synonym="CT122; KPL2; SPGF43"
                     /note="major polyA site"
ORIGIN      
        1 ctttcgccta gttccagcct ggatacgctt ccatagcaaa gggttgccct tggctacagg
       61 aggacgcggg ctggcaggct tggttcctgg cgagtttcta agcccccgcc tgcggtctga
      121 ggcaccggct gaaccatgtc ggagatcctg tgccagtggc tcaacaagga gttgaaggtg
      181 tcccggaccg tgagtcccaa gtcatttgca aaggcatttt ccagtggcta tctacttgga
      241 gaagttctac acaagtttga acttcaggat gatttttcag aatttttgga cagcagggtt
      301 tcaagtgcca aacttaataa tttttctcgc ttggagccaa cacttaacct tctgggtgtg
      361 cagtttgatc agaatgtggc ccatggcatc atcacagaaa agcctggggt ggcaacaaag
      421 ctgttatatc aattgtacat tgctcttcag aaaaagaaga aaagtggact gactggagtg
      481 gagatgcaaa ccatgcaacg tctgacaaat ttaagacttc aaaacatgaa aagtgatact
      541 tttcaagaga gacttagaca catgatacca cgtcaaactg atttcaatct gatgcggatt
      601 acatacaggt ttcaagaaaa atataaacac gtgaaagaag atcttgccca tttgcatttt
      661 gagaaacttg aaagatttca aaaactcaag gaagagcaaa gatgttttga tattgaaaag
      721 caatacttaa acagaagacg acaaaatgaa ataatggcca aaatccaagc agctattata
      781 cagattccta aacctgcatc aaatcgtact ttgaaagcac tcgaggccca aaaaatgatg
      841 aaaaagaaaa aagaggcaga agatgtggct gatgaaatta agaagttcga agcattaata
      901 aaaaaggatc tccaagcaaa agaaagtgca tccaagactt ctttagatac agcaggccag
      961 acaaccaccg atttgttaaa tacttactca gatgacgagt acattaaaaa aatccagaag
     1021 cgccttgaag aagatgcttt tgcacgagag caaagggaga aaagacggcg gaaattgtta
     1081 atggaccagt taatagccca cgaagcacaa gaggaggctt atcgggagga acagctgatt
     1141 aaccggctga tgcggcagtc ccagcaggag cgcaggattg ccgtgcagct catgcatgtt
     1201 cggcatgaaa aggaagtttt atggcaaaac agaattttca gagaaaaaca acatgaggaa
     1261 agacgactta aagatttcca ggatgctctt gatcgagaag cggctttggc aaaacaagcc
     1321 aagattgact ttgaagaaca attccttaaa gaaaagagat ttcatgatca gattgctgtg
     1381 gaaagagctc aagctcgtta tgaaaagcat tattcagtat gtgcagaaat tttggatcaa
     1441 atagttgatt tgtccactaa agtggcagac tatcgaatgt tgacaaataa tctgattccg
     1501 tataagttga tgcatgattg gaaggaacta ttttttaatg caaaacccat atatgaacaa
     1561 gcctctgtta agacactacc tgctaacccc tcaagagaac aacttacaga actggagaaa
     1621 agggacttgc tagataccaa tgattatgaa gaatataaga acatggttgg agagtgggcc
     1681 ttaccagaag aaatggttga caatttacca ccctccaaca attgcatact gggccatatt
     1741 cttcacaggc tagctgaaaa atctcttcct cctcgagcgg aatcaacaac acctgaatta
     1801 ccttcatttg ctgttaaagg atgcttattg gggaaaacat taagtggaaa aactaccatt
     1861 ttaaggtctc tacaaaaaga ctttcctata cagatacttt ctattgacac tcttgtccaa
     1921 gaagctatcc aagcatttca tgacaatgaa aaagtcagtg aggttctacc aattcagaaa
     1981 aatgatgaag aagatgctct accagttctg caagaggaga ttaaagaaag ccaggatcca
     2041 caacatgtat tttcagctgg tccagtttca gatgaagtat taccagaaac agaaggtgaa
     2101 acaatgctta gtgctaatgc tgataaaaca ccaaaagctg aagaagtcaa atcaagtgat
     2161 agtttcttaa aactcactac acgtgctcag cttggtgcaa aatcagaaca gttgctgaag
     2221 aaaggaaaga gcattcctga tgtgctgctt gttgacatca tagtaaatgc tattaatgag
     2281 atacctgtga atcaagactg tatcctagat ggttttccaa tgactttaaa ccaagcacag
     2341 cttctggaag aagctcttac aggctgcaat agaaacctca cagaagtgga aagaaaaaaa
     2401 gcacaaaaat ccacattggc tattgatcct gcgacttcca aagaaatacc tcttccctct
     2461 cctgcatttg attttgtcat attattagat gtttcagata cttcctcaat gagtcgcatg
     2521 aatgatatta tagctgaaga attgtcctat aaaactgctc acgaagatat cagtcaacgt
     2581 gtagctgctg aaaaccaaga taaggatgga gaccaaaatt taagagacca gatacaacat
     2641 agaattatag gcttcttgga caactggcct ttattggagc aatggttttc agagccagaa
     2701 aatattttga taaaaatcaa tgctgaaata gataaggaat ctttatgtga aaaagtaaaa
     2761 gaaattctta cgactgaaat agcaaaaaaa aagaataaag ttgagaagaa attagaagaa
     2821 aaggaagctg agaaaaaagc agcagcttcc ctggctgagc ttccacttcc tacacctcct
     2881 cctgctcctc ctcctgaacc agaaaaagag aaggaaattc atcaaagcca tgtggcttca
     2941 aaaactccta ctgcaaaagg aaaacctcaa tcagaagccc cgcatggtaa gcaagaatct
     3001 cttcaggaag gaaaagggaa gaaaggtgag accgcactca aaagaaaagg ttctcctaaa
     3061 ggaaaatcat caggaggaaa agtaccagta aagaaatcac ctgctgactc tacagataca
     3121 tcacctgttg caatagtgcc acagccacct aagccaggat cagaagaatg ggtctatgtg
     3181 aatgaaccag ttcctgagga aatgcctttg tttttagtac cttactggga actaatagaa
     3241 aattcctata taaacaccat caaaacagta ctcaggcatc tgagggaaga ccagcatact
     3301 gtgcttgctt acttatatga gattagaaca agtttccagg agtttctaaa gcgtccggat
     3361 cacaagcaag attttgtagc tcaatggcag gctgatttca actcccttcc tgatgacctg
     3421 tgggatgatg aggaaacaaa ggctgaacta catcaacgag tgaatgatct gcgagaccgc
     3481 ctgtgggaca tttgtgatgc ccggaaggaa gaggcggagc aggagcggct tgacatcatt
     3541 aatgagagct ggttacagga cactcttgga atgacaatga accatttctt ttccctgatg
     3601 caggcagagc tgaaccgttt ccaagataca aagagactcc ttcaagatta ttactgggga
     3661 atggaaagta aaatcccagt agaggacaac aagagattta ctcgaatccc tttggtccaa
     3721 ctggatagta aagacaattc tgaaagccag cttagaattc ctctagttcc tcgaatatcc
     3781 atttctctgg aaacagttac acccaaacca aaaacaaaat cagtactgaa gggcaagatg
     3841 gataactccc tagaaaacgt tgagtccaac tttgaggccg atgaaaagtt ggtcatggac
     3901 acctggcagc aggcttcttt agcagtatct cacatggtgg ctgctgaaat tcatcagagg
     3961 cttatggaag aagaaaaaga aaaccagcca gcagacccca aagaaaaatc tcctcagatg
     4021 ggtgcaaata aaaaagtcaa aaaggagcca cccaagaaaa aacaggaaga caaaaaaccc
     4081 aaaggtaaat caccacctat ggcagaagca actcctgtca tagtaacaac agaggaaatt
     4141 gctgaaatca aaaggaaaaa tgaactgagg gtcaaaataa aagaagaaca ccttgctgcc
     4201 ttgcaatttg aagaaatagc cacgcaattt cgacttgaac tgataaagac aaaagcattg
     4261 gctcttcttg aagatttagt aacaaaggtg gttgatgtat ataaactcat ggaaaaatgg
     4321 cttggtgaga ggtatttgaa tgaaatggcc agtacagaaa aattaactga cgtagctcgc
     4381 tatcacattg aaacatctac aaaaattcag aatgaacttt atttaagcca agaagacttc
     4441 ttcattaatg gcaatataaa agtcttccca gatcctcccc catcaatacg tcctccacct
     4501 gtagaaaagg aagaagatgg taccctgacc attgaacagc ttgacagtct tcgagatcag
     4561 ttcttagata tggcacctaa aggcataata ggaaataaag catttactga cattctgatc
     4621 gatttggtga ccctgaacct tggcacaaac aactttccta gtaattggat gcaccttacc
     4681 caacctgaat tacaggaatt aacatcttta ttaacagtca actccgagtt cgtggactgg
     4741 cggaagttcc tgttagtaac ctcaatgcct tggcccattc ccttggagga ggagctcctt
     4801 gagacccttc agaagttcaa ggctgtggat aaggagcagt tgggcaccat cacttttgag
     4861 cagtatatgc aggctggtct gtggtttaca ggagatgaag atataaaaat tccagaaaat
     4921 cctcttgaac cccttccatt taataggcag gagcatctta tagagttttt ctttaggcta
     4981 tttgctgact atgagaagga tccaccccag cttgactaca cacagatgct gctttacttt
     5041 gcttgccacc cagacaccgt ggaaggagtc tacagggccc tcagtgtggc tgttggaact
     5101 catgtcttcc aacaagtcaa agcttccatt ccaagtgcag aaaagacctc ctcaactgat
     5161 gcaggtccag ctgaggaatt tcctgaacct gaggaaaatg ctgcaagaga agaaaggaaa
     5221 ttaaaagacg acacggagaa aagggaacag aaggatgaag aaatccctga aaatgcaaac
     5281 aatgaaaaga tgtccatgga aacactactc aaagtgttca aagggggaag tgaagcacag
     5341 gactccaata gatttgccag ccacctaaag atagagaaca tttatgcaga gggcttcata
     5401 aaaacatttc aagacctagg tgccaagaac ctggagccaa ttgaagtcgc tgttctcttg
     5461 aagcatcctt ttattcaaga cctgatttca aattattcag actataagtt tcctgatatt
     5521 aaaataattc tccaaaggag tgaacatgta caaggaagtg atggagagag atcaccttca
     5581 agacatacag aggaaaagaa atgaagacaa aagagtgtga tttttttaat tctgcaataa
     5641 atcttccaaa aattaaatgt ga
//
