LOCUS       NM_015417               1580 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens sperm flagellar 1 (SPEF1), mRNA.
ACCESSION   NM_015417
VERSION     NM_015417.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Tapia,R. and Hecht,G.A.
  TITLE     Spef1/CLAMP binds microtubules and actin-based structures and
            regulates cell migration and epithelia cell polarity
  JOURNAL   Ann N Y Acad Sci 1515 (1), 97-104 (2022)
   PUBMED   35710871
  REMARK    GeneRIF: Spef1/CLAMP binds microtubules and actin-based structures
            and regulates cell migration and epithelia cell polarity.
            Review article
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1580)
  AUTHORS   Tapia,R., Perez-Yepez,E.A., Carlino,M.J., Karandikar,U.C.,
            Kralicek,S.E., Estes,M.K. and Hecht,G.A.
  TITLE     Sperm Flagellar 1 Binds Actin in Intestinal Epithelial Cells and
            Contributes to Formation of Filopodia and Lamellipodia
  JOURNAL   Gastroenterology 157 (6), 1544-1555 (2019)
   PUBMED   31473225
  REMARK    GeneRIF: Sperm flagellar 1 (also called CLAMP) is an actin-binding
            protein, rather than a microtubule-binding protein, in intestinal
            epithelial cells (IECs). CLAMP distribution changes during
            intestinal epithelial cell polarization, regulates the formation of
            filopodia, and appears to assist in the organization of actin
            bundles within lamellipodia of migrating IECs.
REFERENCE   4  (bases 1 to 1580)
  AUTHORS   Werner,M.E., Mitchell,J.W., Putzbach,W., Bacon,E., Kim,S.K. and
            Mitchell,B.J.
  TITLE     Radial intercalation is regulated by the Par complex and the
            microtubule-stabilizing protein CLAMP/Spef1
  JOURNAL   J Cell Biol 206 (3), 367-376 (2014)
   PUBMED   25070955
REFERENCE   5  (bases 1 to 1580)
  AUTHORS   Dougherty,G.W., Adler,H.J., Rzadzinska,A., Gimona,M., Tomita,Y.,
            Lattig,M.C., Merritt,R.C. Jr. and Kachar,B.
  TITLE     CLAMP, a novel microtubule-associated protein with EB-type calponin
            homology
  JOURNAL   Cell Motil Cytoskeleton 62 (3), 141-156 (2005)
   PUBMED   16206169
  REMARK    GeneRIF: a.k.a. Spef1 (sperm flagella) and CLAMP (CaLponin-homology
            And Microtubule associated Protein) CLAMP/Spef1 expression also in
            brain, lung, and inner ear MT association of CLAMP requires the CH
            domain, which has highest similarity with EB family proteins
            Erratum:[Cell Motil Cytoskeleton. 2005 Dec;62(4):259]
REFERENCE   6  (bases 1 to 1580)
  AUTHORS   Chan,S.W., Fowler,K.J., Choo,K.H. and Kalitsis,P.
  TITLE     Spef1, a conserved novel testis protein found in mouse sperm
            flagella
  JOURNAL   Gene 353 (2), 189-199 (2005)
   PUBMED   15979255
REFERENCE   7  (bases 1 to 1580)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB030684.1, DB033051.1, AL040853.1, AL080154.1 and AI925267.1.
            
            On Nov 22, 2018 this sequence version replaced NM_015417.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EF560745.1, BI520334.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968968,
                                           SAMEA1970526 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379756.3/ ENSP00000369080.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               DB030684.1         19-137
            120-576             DB033051.1         120-576
            577-893             AL040853.1         198-514
            894-1304            AL080154.1         1027-1437
            1305-1580           AI925267.1         11-286              c
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..1580
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /note="sperm flagellar 1"
                     /db_xref="GeneID:25876"
                     /db_xref="HGNC:HGNC:15874"
                     /db_xref="MIM:610674"
     exon            1..270
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             162..872
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /note="calponin-homology and microtubule-associated
                     protein"
                     /codon_start=1
                     /product="sperm flagellar protein 1"
                     /protein_id="NP_056232.2"
                     /db_xref="CCDS:CCDS13063.2"
                     /db_xref="GeneID:25876"
                     /db_xref="HGNC:HGNC:15874"
                     /db_xref="MIM:610674"
                     /translation="MASSVDEEALHQLYLWVDNIPLSRPKRNLSRDFSDGVLVAEVIK
                     FYFPKMVEMHNYVPANSLQQKLSNWGHLNRKVLKRLNFSVPDDVMRKIAQCAPGVVEL
                     VLIPLRQRLEERQRRRKQGAGSLQELAPQDGSGYMDVGVSQKARGEGVPDPQGGGQLS
                     WDRPPAPRPPAYNRALQGDPSFVLQIAEKEQELLASQETVQVLQMKVRRLEHLLQLKN
                     VRIEDLSRRLQQAERKQR"
     exon            271..382
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /inference="alignment:Splign:2.1.0"
     exon            383..539
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /inference="alignment:Splign:2.1.0"
     exon            540..579
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /inference="alignment:Splign:2.1.0"
     exon            580..640
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /inference="alignment:Splign:2.1.0"
     exon            641..764
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /inference="alignment:Splign:2.1.0"
     exon            765..1580
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1558..1563
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /note="hexamer: ATTAAA"
     polyA_site      1580
                     /gene="SPEF1"
                     /gene_synonym="C20orf28; CLAMP; SPEF1A"
                     /note="major polyA site"
ORIGIN      
        1 agaaaacaat atggccgctc ccagctggga cgtgagtctc tggattaggc aggcagaggc
       61 agttatgggc ttctgcagtg gcgagaaagg aagcgcctga agggtcgtga ggctggggcg
      121 ggacccggca ccgctggggc ggccaggccg tgaggacgcc aatggcgagc agcgtggacg
      181 aggaggcgct gcaccagctg tacctgtggg tagacaacat ccctctgtcc cggcccaagc
      241 gaaacctctc ccgggacttt agcgatggag tccttgttgc agaggtcatc aagttttact
      301 tccccaagat ggtggagatg cacaattatg tccccgccaa ctctctccag cagaagctca
      361 gcaactgggg tcatctgaac aggaaggtac tgaagaggct gaacttttca gtaccggatg
      421 acgtgatgcg caagatcgcg cagtgcgccc caggcgtggt ggagctggtg ctcatcccgc
      481 tgaggcagcg cctggaggag aggcagaggc gcaggaagca gggcgccggc tccttacagg
      541 agctggctcc ccaggatggc agtggctaca tggatgtggg tgtatcccag aaggcccgag
      601 gtgaaggtgt cccggacccc cagggagggg gtcagctcag ctgggaccgg ccgccggcgc
      661 ctcggcctcc agcgtataac cgggcgttgc agggcgaccc cagcttcgtc ctccagatcg
      721 ctgaaaagga gcaggagctg ttggcctctc aagagaccgt gcaggtcctg cagatgaagg
      781 taaggcgcct ggagcacctg ctccagctca agaatgtgcg gatcgaagac ctctcccggc
      841 ggctccagca ggcggagcgt aagcagcggt gagcggcggc ccgggccgcg cggggacgcc
      901 cgggtacccg ccagagcccc gacgccgcgc cggacccacc caccgatgga tagaccattg
      961 ggagggcgga gcccgctgct ctcacgagcc tgctggggcc cgagtgccct ccttccttgg
     1021 gatgggtgag cgtgggagga gatgggacag gaactctagg agcgcaggcc cgggactgag
     1081 ccgcctccta ccactccgga gatccgggtc aggagaatgg accgctttcc agagcccaga
     1141 agccacgtgc agagacctag cctgtccccc aaagcagtgt ccaacacctt gggcccggcc
     1201 ttgcatctcc cggcgctggg ccttgggggg cggtcccttg gctctgtcca cacccccaga
     1261 atcaggtccc cgcccagctc cgaggacggc ggcgtctcca tccaggctag ttccccatgc
     1321 cctcagccat gggggaatct gtcccgggcc gctgaggggc tcccctgccc ctcctgggag
     1381 cttacctggg acccacctcg gcgacggaga ccgcagcagc tggagaggaa ggggtgaggc
     1441 gtgggatcgc caggagtagg gaggacatcg acgatgtgcc cgtagcagtc gcccctccct
     1501 cctcgcgcac ggggtactga ggcggaaggt ttgaaggtta cggctcaggg ctgccccatt
     1561 aaagtcagtg ttgtgttcta
//
