LOCUS       NM_021928               4569 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens signal peptidase complex subunit 3 (SPCS3), mRNA.
ACCESSION   NM_021928
VERSION     NM_021928.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Liaci,A.M., Steigenberger,B., Telles de Souza,P.C., Tamara,S.,
            Grollers-Mulderij,M., Ogrissek,P., Marrink,S.J., Scheltema,R.A. and
            Forster,F.
  TITLE     Structure of the human signal peptidase complex reveals the
            determinants for signal peptide cleavage
  JOURNAL   Mol Cell 81 (19), 3934-3948 (2021)
   PUBMED   34388369
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4569)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 4569)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 4569)
  AUTHORS   Chen,Q., Denard,B., Lee,C.E., Han,S., Ye,J.S. and Ye,J.
  TITLE     Inverting the Topology of a Transmembrane Protein by Regulating the
            Translocation of the First Transmembrane Helix
  JOURNAL   Mol Cell 63 (4), 567-578 (2016)
   PUBMED   27499293
REFERENCE   6  (bases 1 to 4569)
  AUTHORS   Hosp,F., Vossfeldt,H., Heinig,M., Vasiljevic,D., Arumughan,A.,
            Wyler,E., Landthaler,M., Hubner,N., Wanker,E.E., Lannfelt,L.,
            Ingelsson,M., Lalowski,M., Voigt,A. and Selbach,M.
  CONSRTM   Genetic and Environmental Risk for Alzheimer's Disease GERAD1
            Consortium
  TITLE     Quantitative interaction proteomics of neurodegenerative disease
            proteins
  JOURNAL   Cell Rep 11 (7), 1134-1146 (2015)
   PUBMED   25959826
REFERENCE   7  (bases 1 to 4569)
  AUTHORS   Argente,J., Flores,R., Gutierrez-Arumi,A., Verma,B.,
            Martos-Moreno,G.A., Cusco,I., Oghabian,A., Chowen,J.A.,
            Frilander,M.J. and Perez-Jurado,L.A.
  TITLE     Defective minor spliceosome mRNA processing results in isolated
            familial growth hormone deficiency
  JOURNAL   EMBO Mol Med 6 (3), 299-306 (2014)
   PUBMED   24480542
REFERENCE   8  (bases 1 to 4569)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   9  (bases 1 to 4569)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   10 (bases 1 to 4569)
  AUTHORS   Kalies,K.U. and Hartmann,E.
  TITLE     Membrane topology of the 12- and the 25-kDa subunits of the
            mammalian signal peptidase complex
  JOURNAL   J Biol Chem 271 (7), 3925-3929 (1996)
   PUBMED   8632014
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK026302.1 and AC019163.8.
            
            On Nov 23, 2018 this sequence version replaced NM_021928.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.97109.1,
                                           SRR1660807.2266.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000503362.2/ ENSP00000427463.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-778               AK026302.1         3-780
            779-4569            AC019163.8         80705-84495
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q34.2"
     gene            1..4569
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
                     /note="signal peptidase complex subunit 3"
                     /db_xref="GeneID:60559"
                     /db_xref="HGNC:HGNC:26212"
                     /db_xref="MIM:618854"
     exon            1..254
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..654
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
                     /note="SPase 22 kDa subunit; microsomal signal peptidase
                     23 kDa subunit; SPase 22/23 kDa subunit; microsomal signal
                     peptidase 22/23 kDa subunit; signal peptidase complex
                     subunit 3 homolog"
                     /codon_start=1
                     /product="signal peptidase complex subunit 3"
                     /protein_id="NP_068747.1"
                     /db_xref="CCDS:CCDS54823.1"
                     /db_xref="GeneID:60559"
                     /db_xref="HGNC:HGNC:26212"
                     /db_xref="MIM:618854"
                     /translation="MNTVLSRANSLFAFSLSVMAALTFGCFITTAFKDRSVPVRLHVS
                     RIMLKNVEDFTGPRERSDLGFITFDITADLENIFDWNVKQLFLYLSAEYSTKNNALNQ
                     VVLWDKIVLRGDNPKLLLKDMKTKYFFFDDGNGLKGNRNVTLTLSWNVVPNAGILPLV
                     TGSGHVSVPFPDTYEITKSY"
     exon            255..328
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
                     /inference="alignment:Splign:2.1.0"
     exon            329..405
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
                     /inference="alignment:Splign:2.1.0"
     exon            406..521
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
                     /inference="alignment:Splign:2.1.0"
     exon            522..4569
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
                     /inference="alignment:Splign:2.1.0"
     regulatory      650..655
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
                     /note="hexamer: ATTAAA"
     polyA_site      677
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
     regulatory      4547..4552
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
                     /note="hexamer: AATAAA"
     polyA_site      4569
                     /gene="SPCS3"
                     /gene_synonym="PRO3567; SPC22; SPC22/23; SPC23; SPC3;
                     YLR066W"
                     /note="major polyA site"
ORIGIN      
        1 gctcccggaa cgcgcgcacc gcagacggcg cggatcgcag ggagccggtc cgccgccgga
       61 acgggagcct gggtgtgcgt gtggagtccg gactcgtggg agacgatcgc gatgaacacg
      121 gtgctgtcgc gggcgaactc actgttcgcc ttctcgctga gcgtgatggc ggcgctcacc
      181 ttcggctgct tcatcaccac cgccttcaaa gacaggagcg tcccggtgcg gctgcacgtc
      241 tcgcggatca tgctaaaaaa tgtagaagat ttcactggac ctagagaaag aagtgatctg
      301 ggatttatca catttgatat aactgctgat ctagagaata tatttgattg gaatgttaag
      361 cagttgtttc tttatttatc agcagaatat tcaacaaaaa ataatgctct gaaccaagtt
      421 gtcctatggg acaagattgt tttgagaggt gataatccga agctgctgct gaaagatatg
      481 aaaacaaaat attttttctt tgacgatgga aatggtctca agggaaacag gaatgtcact
      541 ttgaccctgt cttggaacgt cgtaccaaat gctggaattc tacctcttgt gacaggatca
      601 ggacacgtat ctgtcccatt tccagataca tatgaaataa cgaagagtta ttaaattatt
      661 ctgaatttga aacaacatat ttttatactt aatgaattgt atctcattaa tctcttccct
      721 tacatcttca tgtattgttg gtttgttttt tggttttggg tttttttttt tttttttttg
      781 gtataagaac taacatcaaa aggcctgttt aaagggaaag gttaatgggc tacttaatat
      841 tatgaacaaa acaaaaaaac aaggctgcca cagtggaata ttatcttaca agaataagaa
      901 ctacataaaa cagatttgta aaaaatacat atttgaagta ttccctgtat ttccattatt
      961 ctttatggaa tataaagtaa gcatgaaagg tagttaaaac tttcaggtgc ctgtagagtc
     1021 ataataactg tattttatgc cttgcattca cgcaaattca cattggatgt gatttaaaag
     1081 tagacattct ctttttcctc ttttaggata tgtttgatta ctggaaaatt aatatggtta
     1141 tttgttagaa gtctggttta taaaaaagcc aaaagtgatg gaatttattc catttgtctt
     1201 aggaaggccc ataatacttg tttttcttac atgtgactag caactttctc cacttaaaga
     1261 ctaaatacct ctttatatga tgtaaattat tctaattcat tttaaaatct tttaggtcag
     1321 caaaatgtgt gtcttcagtg ctttctctaa aaacgttcct ttatagcttt gtactttttt
     1381 ttttagcgtt gccattgaaa gttaaaatgt ttgcatggtt taccctctga gttatgtttc
     1441 ttctagtgag catgcctgct gtcactaagt gaattattta ctactttttg taggtctata
     1501 ttttaataat tattgggata ataataattg tggtatgctt tccatttaca aaccactcat
     1561 atactttagt gtatgtcatc ctcaataatc taatgaggta agtagtataa gtgttagctc
     1621 tttgcagata agaaactgaa acccagaagt ttattgaatt gcttcattgt tacctagcca
     1681 gcaaggagtg gaattaaaac ttgaactcag atcttctaat tttctgagct catcctttct
     1741 cttatacctc agattgcaga ctaatcaaat tttacttaaa accttcctga tatttttgtt
     1801 accctggaca ccctttctat tctagaaaag gtttcccatt ttatatggaa gtgactaatt
     1861 ttcaagctgc caacaactta tatcgaggta atattttatt ctctgaataa attcatttca
     1921 cttatattaa aagcataagg aaatctacat gtatgtaaaa ataacttaga aattcagtac
     1981 ataatacatg attgaataca tgatcgtatt taacatgttt tttttttctg cagtggacaa
     2041 ataaacatcc tcaaagtagc aactgcaaat cagttaccct tagaaaagca agaccaaaca
     2101 ctgtagttac actattagca gtgaccaaaa agggctaata ttttctaaga atagtttaaa
     2161 ttacagacat ttgttatatt taccttatgt gaaatacatc actatttaat tacattaatt
     2221 ttaacatctg ttgtgtggag ttgtatagtt catgcaaaag cctgtgggta tgggtttttc
     2281 aaaccagcag aaaggtcaaa ggtacctgaa tgctaaactg cctggctccc agctttttca
     2341 ttaaactttt cagggtcttg gtttctttat ctgtaaaatg acagagttgg accagttaac
     2401 tttaatggcc atccttttac accacacaag ttgataaaat ttatctgttc agcaaagaga
     2461 ttgaacaaaa aagcacgtta gtaatatgaa gacaggaaaa cgaatgaaag tctaacacat
     2521 aactcatatt gatttacttt atttctgtta gattttacac tctgaaaatt tcacctcatt
     2581 tagtttgtac aaatactaga catggaaact taaaatgtgc aggtgtcaaa gcttaaaaat
     2641 caggtgtgtc ctctttaaag gcatgtgcga taagcctgca gtcttaacca gacctggtac
     2701 cagtttaaca gttcatgata cttctgtgat gagtggatct gttagtctgg tagagattaa
     2761 tgttgaaatt tacttgaatt acagttattt gacaagccca ctttttttgg agtttggtga
     2821 ggaagataag taaaaatgcc caactagtac aatgtggaga aagttttctg tttgcttgac
     2881 taagggaaat tgctcagaaa aagaatgctc caacatggga ataaggacct tcagtctcag
     2941 gaactcattt ttgggtgcag tttctacatt taaaaaattg acttgtgttc agtttgataa
     3001 ccagttcatt tgctttagtc tcagtgtttt aaagaagtct cataatgttc tacaaagaca
     3061 caaagcttca ggcttataca aaactgagta tgattagaaa tacctgagcc cagaaatgat
     3121 tctgagaaaa gagaataatt tgaagacact tatttaaaag taattatggt tagaaatgaa
     3181 ttaatttaaa aatgtgttca catatccctt tctctaacag tttaacctag acaaacatct
     3241 gtatcagtat ttttttattc ccctgattga ttacatttgg tttctttatt ctgaaaggaa
     3301 aataacaaaa acttcagaaa ttcctaaggg tgtaataaga aagtgggttt tgagtttcct
     3361 ttcctggaat tattttacag ttctttggtg ggtctcgtca gccaattcat agctgggctt
     3421 taagattatc atcttagaga tgatagatgg gctttttaaa gatttcatct tagagatgaa
     3481 aagctgggct ttttctctaa gatgataatc ttaaagccca gctattaatt gataatgaac
     3541 atttttcact attttttttt ctatctgaag cttagagatc tagagctttg gatctttcgg
     3601 gtatatgtca atggaggtat tattttataa tactttgcat tgacatgaag tgggttcatg
     3661 ggggaaaacc atgagctgtg aacattggta gcaaacaagc atatattcat ttcaaaactt
     3721 tccttgcttt tagcagagag aagcctgtat atgttacatg tgtgactttc agtagtttaa
     3781 agagatgttt caaaaaattg ttgcatgttt ttgatgcaat ttgggaaatt gtttacttca
     3841 caatgtagtc attcataaaa aaattcatga aaatactgaa catatgtttg aggatttttc
     3901 ttttcctttt taaatttttt tattttttct gagacggaga tctgctctta cgcccaggct
     3961 agagtgaagt ggcgcgatct tggcttactg caacctccac cccccaggtt caagcgattc
     4021 tcctgcctca gcctccggag tagctgggat tacaggcgcc cgccaccacg tccggctaat
     4081 ttttgtattt tcagtagaga cggggttttg ctatgttggc caggctggtc tcaaactcct
     4141 gacctcaagt gatccacctg cctcggcctc ccaaagtgtt aggataacag gtgtgagcca
     4201 ccgtgcccgg ctgaagattt ttcttaattg caataaatat tcagcatttt ttctaatgaa
     4261 aatgaatttt gtttaccagt aaaagtatgc attttaaaag actttcagat ttatgctttt
     4321 tacgtgaagc tgctaaacta aaagtaaatg gaagaaacca agtctagtag gttttttctt
     4381 tttttttgtg ggggtgggat gggggaggtt agttacactt aaaatatctt ctccagagac
     4441 tgtatgctcc tatactagac tgtaagctct ttgagggcag tctgtcagat ttatctttgt
     4501 atcttcccca gcgcctagtg tagtgccttg cacataatag gcgcccaata aatattgatg
     4561 aagaatgaa
//
