LOCUS       NM_014752               2691 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens signal peptidase complex subunit 2 (SPCS2), mRNA.
ACCESSION   NM_014752
VERSION     NM_014752.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Liaci,A.M., Steigenberger,B., Telles de Souza,P.C., Tamara,S.,
            Grollers-Mulderij,M., Ogrissek,P., Marrink,S.J., Scheltema,R.A. and
            Forster,F.
  TITLE     Structure of the human signal peptidase complex reveals the
            determinants for signal peptide cleavage
  JOURNAL   Mol Cell 81 (19), 3934-3948 (2021)
   PUBMED   34388369
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Chen,Z., Wang,C., Feng,X., Nie,L., Tang,M., Zhang,H., Xiong,Y.,
            Swisher,S.K., Srivastava,M. and Chen,J.
  TITLE     Interactomes of SARS-CoV-2 and human coronaviruses reveal host
            factors potentially affecting pathogenesis
  JOURNAL   EMBO J 40 (17), e107776 (2021)
   PUBMED   34232536
REFERENCE   3  (bases 1 to 2691)
  AUTHORS   Argente,J., Flores,R., Gutierrez-Arumi,A., Verma,B.,
            Martos-Moreno,G.A., Cusco,I., Oghabian,A., Chowen,J.A.,
            Frilander,M.J. and Perez-Jurado,L.A.
  TITLE     Defective minor spliceosome mRNA processing results in isolated
            familial growth hormone deficiency
  JOURNAL   EMBO Mol Med 6 (3), 299-306 (2014)
   PUBMED   24480542
REFERENCE   4  (bases 1 to 2691)
  AUTHORS   Kalies,K.U. and Hartmann,E.
  TITLE     Membrane topology of the 12- and the 25-kDa subunits of the
            mammalian signal peptidase complex
  JOURNAL   J Biol Chem 271 (7), 3925-3929 (1996)
   PUBMED   8632014
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP338843.1, D14658.1, AP001992.4 and AA921960.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014752.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.272124.1,
                                           SRR1803611.112664.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263672.11/ ENSP00000263672.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BP338843.1         20-41
            23-1392             D14658.1           1-1370
            1393-2402           AP001992.4         126046-127055
            2403-2689           AA921960.1         24-310              c
            2690-2691           AP001992.4         127343-127344
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.4"
     gene            1..2691
                     /gene="SPCS2"
                     /note="signal peptidase complex subunit 2"
                     /db_xref="GeneID:9789"
                     /db_xref="HGNC:HGNC:28962"
                     /db_xref="MIM:619411"
     exon            1..134
                     /gene="SPCS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..701
                     /gene="SPCS2"
                     /EC_number="3.4.-.-"
                     /note="signal peptidase 25kDa subunit; SPase 25 kDa
                     subunit; microsomal signal peptidase 25 kDa subunit;
                     signal peptidase complex subunit 2 homolog"
                     /codon_start=1
                     /product="signal peptidase complex subunit 2"
                     /protein_id="NP_055567.2"
                     /db_xref="CCDS:CCDS44681.1"
                     /db_xref="GeneID:9789"
                     /db_xref="HGNC:HGNC:28962"
                     /db_xref="MIM:619411"
                     /translation="MAAAAVQGGRSGGSGGCSGAGGASNCGTGSGRSGLLDKWKIDDK
                     PVKIDKWDGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVALIWD
                     YMHPFPESKPVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQLS
                     SSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYEPEISRLHDS
                     LAIERKIK"
     exon            135..218
                     /gene="SPCS2"
                     /inference="alignment:Splign:2.1.0"
     exon            219..379
                     /gene="SPCS2"
                     /inference="alignment:Splign:2.1.0"
     exon            380..514
                     /gene="SPCS2"
                     /inference="alignment:Splign:2.1.0"
     exon            515..2691
                     /gene="SPCS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      692..697
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPCS2"
                     /note="hexamer: AATAAA"
     polyA_site      716
                     /gene="SPCS2"
                     /note="major polyA site"
     regulatory      1265..1270
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPCS2"
                     /note="hexamer: ATTAAA"
     polyA_site      1294
                     /gene="SPCS2"
     regulatory      1372..1377
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPCS2"
                     /note="hexamer: AATAAA"
     polyA_site      1399
                     /gene="SPCS2"
     regulatory      2666..2671
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPCS2"
                     /note="hexamer: ATTAAA"
     polyA_site      2691
                     /gene="SPCS2"
ORIGIN      
        1 gagacgcaga ggcggacaag atggcggcgg cagctgtaca gggcgggaga agcggtggta
       61 gcggaggctg tagtggggct ggtggtgctt ccaactgcgg gacaggaagt ggccgtagcg
      121 gcttgttgga taagtggaag atagatgata agcctgtaaa aattgacaag tgggatggat
      181 cagctgtgaa aaactctttg gatgattctg ccaaaaaggt acttctggaa aaatacaaat
      241 atgtggagaa ttttggtcta attgatggtc gcctcaccat ctgtacaatc tcctgtttct
      301 ttgccatagt ggctttgatt tgggattata tgcacccctt tccagagtcc aaacccgttt
      361 tggctttgtg tgtcatatcc tattttgtga tgatggggat tctgaccatt tatacctcat
      421 ataaggagaa gagcatcttt ctcgtggccc acaggaaaga tcctacagga atggatcctg
      481 atgatatttg gcagctgtcc tccagtctta aaaggtttga tgacaaatac accttgaagc
      541 tgaccttcat cagtgggaga acaaagcagc agcgggaagc cgagttcaca aagtccattg
      601 ctaagttttt tgaccacagt gggacactgg tcatggatgc atatgagcct gaaatatcca
      661 ggctccatga cagtcttgcc atagaaagaa aaataaagta gccaattcta aaagtagccc
      721 tctttctcct ggatcttgct gaattagtgg cttggggggt gggggagata aaaagaactt
      781 aaaatgggta aagtaagaaa tgttaaaaag tccctgtttt gtcctgaaat tttagtctat
      841 tctgggtaaa taggattttc tgacacagat atgagaagtt gtagctctga tgtctagctg
      901 tagtctcctt gatctgctga ttgcattatt ttaatttgct tttctgggaa agcagttttg
      961 ctaaaagctg tacagacttt ttcttttgta cctagcagta ctttatatag tatagctttg
     1021 ggccatgtag cattttaaga ctcaatttta aaaaattatt aatctgttgc tgactcttaa
     1081 ttcctatttc aatatgtgtt tccttgaaga attcaggata caacttcttg tgtatgacag
     1141 ctttccttca cacactattt ttgtgggtgt gtatatatct gatttgggaa gaatttaaaa
     1201 aacacatagc tttttaattt gtttgaaaca gactttctgc ctgttacatt tttgctttta
     1261 accaattaaa gaagccaatg gcattttagt tttatattgt gttttccact agtatatccc
     1321 tgttgatttg tttgtgcctt ttattaactg ccattttcta aaattttttt caataaaagg
     1381 aaggaagatg tgaaaaaaat ggagtcatag ttttgatgga gagaacagag aaacaagtgt
     1441 taacatcttt ctactatagg tttttttcat ttttaggcta ttttacttgt caaaggcccc
     1501 tgagaacctg tagctaagga actcttagct ttccagtgat aataacaata ataacaacat
     1561 tctgtactta ctttgtgcca gaaatgctcc aagtacttta catgtgttaa taatgctcac
     1621 agcaatccta agaggcaggt gctgttatta tccccatttt acacatgagg aaactgagat
     1681 acaaaagtta aataattgtc acacagctag tgatagaact gggattcaaa cctggggagt
     1741 ccggcttcag agtttgtggt cctgcagaga caaaacagcc gcttttccag tattttccca
     1801 gtatggtcag agaagacctg ggtgcttgcc aagatccttt gacctttagg aatatttccc
     1861 tacagcctta caaaacatca attttggctg agcctagtac ccaacactat attcagtttg
     1921 taagaaatta ccattatcac atttcctgtt tgtcttggag aggttgtcct atctcgcacc
     1981 acatggtagg gaattgtgaa caacactgga cagaaagtta aagccttgga ttctgtactc
     2041 ttcattcctg taaaccttgg gtaagtttcg gaattctctg tgagcttctt aaagtttttg
     2101 aaagcatgta accggataag tgtattttta caatggagga gttacagtca tcttggtatt
     2161 tgtgggagat tggttccagg atccccaagc ataccaaaat ccaaggatgc tcaagtccct
     2221 tatataaaat ggtatagcat ttgcatataa cctacacaca tcctcccata tacttttaat
     2281 cacctctgga ttacctataa tacctaatat aatgtaaatg ctatgtaaat agttatactg
     2341 cattggtttt ttaatttata ttttttgtta ttttttaaaa tatattttta atccatggtt
     2401 ggttaaatct gtagatatag agggccaact gtactgttag acacaagggt ctcgaggctt
     2461 gcagatagag actgaataac cctctccatg atgatccctt agagttcccc cactgtgcct
     2521 ttttctttac atgtgcatgc aaacatgtgt ctccacatac atttttttta acaaaaattg
     2581 aattatgttg atatatgtcc tgtaatttgt gttttaaact taatatatct taggcatttt
     2641 ccctgtgtgt gtgtataatg cagctattaa aaatgagtct gaggccaggc a
//
