LOCUS       NM_020675               1342 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens SPC25 component of NDC80 kinetochore complex (SPC25),
            mRNA.
ACCESSION   NM_020675
VERSION     NM_020675.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Zhang,W., Yu,L., Xu,C., Tang,T., Cao,J., Chen,L., Pang,X. and
            Ren,W.
  TITLE     PLEK2 activates the PI3K/AKT signaling pathway to drive lung
            adenocarcinoma progression by upregulating SPC25
  JOURNAL   Cell Biol Int 48 (9), 1285-1300 (2024)
   PUBMED   38894536
  REMARK    GeneRIF: PLEK2 activates the PI3K/AKT signaling pathway to drive
            lung adenocarcinoma progression by upregulating SPC25.
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Liao,L.X., Zhang,M., Xu,X., Zhang,S. and Guo,Y.Z.
  TITLE     SPC25 Functions as a Prognostic-Related Biomarker, and Its High
            Expression Correlates with Tumor Immune Infiltration and UCEC
            Progression
  JOURNAL   Front Biosci (Landmark Ed) 29 (2), 69 (2024)
   PUBMED   38420826
  REMARK    GeneRIF: SPC25 Functions as a Prognostic-Related Biomarker, and Its
            High Expression Correlates with Tumor Immune Infiltration and UCEC
            Progression.
REFERENCE   3  (bases 1 to 1342)
  AUTHORS   Liu,X.S., Zhang,Y., Ming,X., Hu,J., Chen,X.L., Wang,Y.L.,
            Zhang,Y.H., Gao,Y. and Pei,Z.J.
  TITLE     SPC25 as a novel therapeutic and prognostic biomarker and its
            association with glycolysis, ferroptosis and ceRNA in lung
            adenocarcinoma
  JOURNAL   Aging (Albany NY) 16 (1), 779-798 (2024)
   PUBMED   38217547
  REMARK    GeneRIF: SPC25 as a novel therapeutic and prognostic biomarker and
            its association with glycolysis, ferroptosis and ceRNA in lung
            adenocarcinoma.
REFERENCE   4  (bases 1 to 1342)
  AUTHORS   Yang,J., Huang,Y., Song,M., Pan,Q., Zhao,J., He,J., Ouyang,D.,
            Yang,C., Han,Y., Tang,Y., Wang,Q., He,J., Li,Y., Chen,H., Weng,D.,
            Xiang,T. and Xia,J.
  TITLE     SPC25 promotes proliferation and stemness of hepatocellular
            carcinoma cells via the DNA-PK/AKT/Notch1 signaling pathway
  JOURNAL   Int J Biol Sci 18 (14), 5241-5259 (2022)
   PUBMED   36147467
  REMARK    GeneRIF: SPC25 promotes proliferation and stemness of
            hepatocellular carcinoma cells via the DNA-PK/AKT/Notch1 signaling
            pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1342)
  AUTHORS   Cui,F., Xu,Z., Hu,J. and Lv,Y.
  TITLE     Spindle pole body component 25 and platelet-derived growth factor
            mediate crosstalk between tumor-associated macrophages and prostate
            cancer cells
  JOURNAL   Front Immunol 13, 907636 (2022)
   PUBMED   35967419
  REMARK    GeneRIF: Spindle pole body component 25 and platelet-derived growth
            factor mediate crosstalk between tumor-associated macrophages and
            prostate cancer cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1342)
  AUTHORS   Ciferri,C., Pasqualato,S., Screpanti,E., Varetti,G., Santaguida,S.,
            Dos Reis,G., Maiolica,A., Polka,J., De Luca,J.G., De Wulf,P.,
            Salek,M., Rappsilber,J., Moores,C.A., Salmon,E.D. and Musacchio,A.
  TITLE     Implications for kinetochore-microtubule attachment from the
            structure of an engineered Ndc80 complex
  JOURNAL   Cell 133 (3), 427-439 (2008)
   PUBMED   18455984
REFERENCE   7  (bases 1 to 1342)
  AUTHORS   Ciferri,C., De Luca,J., Monzani,S., Ferrari,K.J., Ristic,D.,
            Wyman,C., Stark,H., Kilmartin,J., Salmon,E.D. and Musacchio,A.
  TITLE     Architecture of the human ndc80-hec1 complex, a critical
            constituent of the outer kinetochore
  JOURNAL   J Biol Chem 280 (32), 29088-29095 (2005)
   PUBMED   15961401
  REMARK    GeneRIF: the Spc24, Spc25, Nuf2, and Ndc80/Hec1 complex is a
            faithful copy of the endogenous Ndc80 complex
REFERENCE   8  (bases 1 to 1342)
  AUTHORS   Bharadwaj,R., Qi,W. and Yu,H.
  TITLE     Identification of two novel components of the human NDC80
            kinetochore complex
  JOURNAL   J Biol Chem 279 (13), 13076-13085 (2004)
   PUBMED   14699129
  REMARK    GeneRIF: hSPC25 is an essential kinetochore component that plays a
            significant role in proper execution of mitotic events
REFERENCE   9  (bases 1 to 1342)
  AUTHORS   McCleland,M.L., Kallio,M.J., Barrett-Wilt,G.A., Kestner,C.A.,
            Shabanowitz,J., Hunt,D.F., Gorbsky,G.J. and Stukenberg,P.T.
  TITLE     The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs,
            which are required to establish and maintain
            kinetochore-microtubule attachment
  JOURNAL   Curr Biol 14 (2), 131-137 (2004)
   PUBMED   14738735
REFERENCE   10 (bases 1 to 1342)
  AUTHORS   Kalies,K.U., Rapoport,T.A. and Hartmann,E.
  TITLE     The beta subunit of the Sec61 complex facilitates cotranslational
            protein transport and interacts with the signal peptidase during
            translocation
  JOURNAL   J Cell Biol 141 (4), 887-894 (1998)
   PUBMED   9585408
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC069137.6.
            
            On Nov 26, 2018 this sequence version replaced NM_020675.3.
            
            Summary: This gene encodes a protein that may be involved in
            kinetochore-microtubule interaction and spindle checkpoint
            activity. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC022255.1, SRR18074967.118523.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000282074.7/ ENSP00000282074.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-113               AC069137.6         59956-60068         c
            114-260             AC069137.6         59025-59171         c
            261-326             AC069137.6         58864-58929         c
            327-473             AC069137.6         46876-47022         c
            474-578             AC069137.6         45710-45814         c
            579-677             AC069137.6         43223-43321         c
            678-1342            AC069137.6         40529-41193         c
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.3"
     gene            1..1342
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /note="SPC25 component of NDC80 kinetochore complex"
                     /db_xref="GeneID:57405"
                     /db_xref="HGNC:HGNC:24031"
                     /db_xref="MIM:609395"
     exon            1..113
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /inference="alignment:Splign:2.1.0"
     exon            114..260
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..802
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /note="2600017H08Rik; spindle pole body component 25
                     homolog; SPC25, NDC80 kinetochore complex component,
                     homolog"
                     /codon_start=1
                     /product="kinetochore protein Spc25"
                     /protein_id="NP_065726.1"
                     /db_xref="CCDS:CCDS2229.1"
                     /db_xref="GeneID:57405"
                     /db_xref="HGNC:HGNC:24031"
                     /db_xref="MIM:609395"
                     /translation="MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFA
                     EKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTAN
                     IQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTN
                     IDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAF
                     TATVYN"
     exon            261..326
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /inference="alignment:Splign:2.1.0"
     exon            327..473
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /inference="alignment:Splign:2.1.0"
     exon            474..578
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /inference="alignment:Splign:2.1.0"
     exon            579..677
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /inference="alignment:Splign:2.1.0"
     exon            678..1342
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      818..823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /note="hexamer: TATAAA"
     polyA_site      845
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /note="major polyA site"
     regulatory      1315..1320
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
                     /note="hexamer: AATAAA"
     polyA_site      1342
                     /gene="SPC25"
                     /gene_synonym="AD024; hSpc25; SPBC25"
ORIGIN      
        1 gaaatcggaa agttggcggg gctgcgggag ctgagcctag agtccggctg ttggctagag
       61 tgggcgcgga tctggtgtgg ggaaggcggc gggactcagg cctgcctgcg aagcattgtc
      121 ctacataatg gtagaggacg aactggcact tttcgataaa agcataaatg aattttggaa
      181 taaattcaaa agtacggaca cctcctgtca gatggcggga ctaagagata cctacaagga
      241 ttccatcaaa gcatttgcag aaaagctgtc tgtgaaatta aaggaagaag aacgaatggt
      301 tgagatgttt ctggaatatc aaaatcagat cagcaggcaa aataagctca ttcaagaaaa
      361 aaaggataac ttgttaaaat tgattgctga agtaaaaggc aaaaagcagg aattggaagt
      421 actgactgca aatatccagg atcttaagga agaatattct aggaagaagg aaactatttc
      481 tactgctaat aaagcgaatg cagagaggtt gaaaaggctg cagaaatctg cagacttgta
      541 taaagatcga cttggactag aaattcgaaa aatttatggt gagaaattgc agtttatttt
      601 cactaatatt gaccctaaga atcctgagag cccatttatg ttttccttac atctcaatga
      661 agcaagggac tatgaagtgt cagatagtgc ccctcatctt gagggcctag cagaatttca
      721 agagaatgta aggaagacca acaatttttc agcttttctt gccaatgttc ggaaagcttt
      781 tactgccacg gtttataatt aacatacaaa tagtgtatat aaaaacggtt tatttttctt
      841 ctctattaca tatctctttt tttcttgttt ttattattat tatactttaa gttttagggt
      901 acatgtgcac aatgtgcagg tttgttacat atgtatacat gtgccatatt ggtgtgctgc
      961 acccattaac tcgtcatttc attaggtata tctcctaatg ctatccctcc cccctccccc
     1021 aacccacaac agtccccgtt gtgtgatgtt ccccttcctg tgtccatgtg ttctcattgt
     1081 tcaattccca cctaggagtg agaatatgtg gtgtttggtt ttttgtcctt tcgatagttt
     1141 gctgagaatg atggtttcca gcttcatcca tgttcctaca aaggacatga actcatcctt
     1201 ttttatggct gcatagtatt ccatggtgta tatgtgccac attttcttaa tccagtctat
     1261 cattgttgga catttgggtt ggttccaagt ctttgctatt gtgaatagtg ccgaaataaa
     1321 catacgtgtg catgtgtctt ta
//
