LOCUS       NM_182513               2290 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens SPC24 component of NDC80 kinetochore complex (SPC24),
            transcript variant 1, mRNA.
ACCESSION   NM_182513
VERSION     NM_182513.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Yue,H., Wu,K., Liu,K., Gou,L., Huang,A. and Tang,H.
  TITLE     LINC02154 promotes the proliferation and metastasis of
            hepatocellular carcinoma by enhancing SPC24 promoter activity and
            activating the PI3K-AKT signaling pathway
  JOURNAL   Cell Oncol (Dordr) 45 (3), 447-462 (2022)
   PUBMED   35543858
  REMARK    GeneRIF: LINC02154 promotes the proliferation and metastasis of
            hepatocellular carcinoma by enhancing SPC24 promoter activity and
            activating the PI3K-AKT signaling pathway.
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Wang,Y., Yang,H., Zhang,G., Luo,C., Zhang,S., Luo,R. and Deng,B.
  TITLE     hsa-miR-7-5p suppresses proliferation, migration and promotes
            apoptosis in hepatocellular carcinoma cell lines by inhibiting
            SPC24 expression
  JOURNAL   Biochem Biophys Res Commun 561, 80-87 (2021)
   PUBMED   34020142
  REMARK    GeneRIF: hsa-miR-7-5p suppresses proliferation, migration and
            promotes apoptosis in hepatocellular carcinoma cell lines by
            inhibiting SPC24 expression.
REFERENCE   3  (bases 1 to 2290)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2290)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2290)
  AUTHORS   Zhou,J., Pei,Y., Chen,G., Cao,C., Liu,J., Ding,C., Wang,D., Sun,L.,
            Xu,P. and Niu,G.
  TITLE     SPC24 Regulates breast cancer progression by PI3K/AKT signaling
  JOURNAL   Gene 675, 272-277 (2018)
   PUBMED   30180968
  REMARK    GeneRIF: SPC24 is up-regulated in human breast cancer.SPC24
            regulates PI3K/AKT kinase pathway in breast cancer.
REFERENCE   6  (bases 1 to 2290)
  AUTHORS   Nousiainen,M., Sillje,H.H., Sauer,G., Nigg,E.A. and Korner,R.
  TITLE     Phosphoproteome analysis of the human mitotic spindle
  JOURNAL   Proc Natl Acad Sci U S A 103 (14), 5391-5396 (2006)
   PUBMED   16565220
REFERENCE   7  (bases 1 to 2290)
  AUTHORS   Ciferri,C., De Luca,J., Monzani,S., Ferrari,K.J., Ristic,D.,
            Wyman,C., Stark,H., Kilmartin,J., Salmon,E.D. and Musacchio,A.
  TITLE     Architecture of the human ndc80-hec1 complex, a critical
            constituent of the outer kinetochore
  JOURNAL   J Biol Chem 280 (32), 29088-29095 (2005)
   PUBMED   15961401
  REMARK    GeneRIF: the Spc24, Spc25, Nuf2, and Ndc80/Hec1 complex is a
            faithful copy of the endogenous Ndc80 complex
REFERENCE   8  (bases 1 to 2290)
  AUTHORS   Ahonen,L.J., Kallio,M.J., Daum,J.R., Bolton,M., Manke,I.A.,
            Yaffe,M.B., Stukenberg,P.T. and Gorbsky,G.J.
  TITLE     Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope
            and modulates the association of spindle-checkpoint proteins at
            kinetochores
  JOURNAL   Curr Biol 15 (12), 1078-1089 (2005)
   PUBMED   15964272
REFERENCE   9  (bases 1 to 2290)
  AUTHORS   Bharadwaj,R., Qi,W. and Yu,H.
  TITLE     Identification of two novel components of the human NDC80
            kinetochore complex
  JOURNAL   J Biol Chem 279 (13), 13076-13085 (2004)
   PUBMED   14699129
  REMARK    GeneRIF: hSPC24 is a component of the human NDC80 kinetochore
            complex
REFERENCE   10 (bases 1 to 2290)
  AUTHORS   McCleland,M.L., Kallio,M.J., Barrett-Wilt,G.A., Kestner,C.A.,
            Shabanowitz,J., Hunt,D.F., Gorbsky,G.J. and Stukenberg,P.T.
  TITLE     The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs,
            which are required to establish and maintain
            kinetochore-microtubule attachment
  JOURNAL   Curr Biol 14 (2), 131-137 (2004)
   PUBMED   14738735
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC011485.6.
            
            On Apr 10, 2021 this sequence version replaced NM_182513.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK075287.1, DB294170.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000592540.6/ ENSP00000465075.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-166               AC011485.6         24128-24293
            167-311             AC011485.6         30672-30816
            312-416             AC011485.6         31793-31897
            417-493             AC011485.6         32016-32092
            494-2290            AC011485.6         32621-34417
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.2"
     gene            1..2290
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /note="SPC24 component of NDC80 kinetochore complex"
                     /db_xref="GeneID:147841"
                     /db_xref="HGNC:HGNC:26913"
                     /db_xref="MIM:609394"
     exon            1..166
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /inference="alignment:Splign:2.1.0"
     CDS             7..600
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kinetochore protein Spc24; hSpc24; spindle pole body
                     component 24 homolog; SPC24, NDC80 kinetochore complex
                     component, homolog"
                     /codon_start=1
                     /product="kinetochore protein Spc24 isoform 1"
                     /protein_id="NP_872319.1"
                     /db_xref="CCDS:CCDS45974.1"
                     /db_xref="GeneID:147841"
                     /db_xref="HGNC:HGNC:26913"
                     /db_xref="MIM:609394"
                     /translation="MAAFRDIEEVSQGLLSLLGANRAEAQQRRLLGRHEQVVERLLET
                     QDGAEKQLREILTMEKEVAQSLLNAKEQVHQGGVELQQLEAGLQEAGEEDTRLKASLL
                     QLTRELEELKEIEADLERQEKEVDEDTTVTIPSAVYVAQLYHQVSKIEWDYECEPGMV
                     KGIHHGPSVAQPIHLDSTQLSRKFISDYLWSLVDTEW"
     exon            167..311
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /inference="alignment:Splign:2.1.0"
     exon            312..416
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /inference="alignment:Splign:2.1.0"
     exon            417..493
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /inference="alignment:Splign:2.1.0"
     exon            494..2290
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1855..1860
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /note="hexamer: ATTAAA"
     polyA_site      1874
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /note="major polyA site"
     regulatory      2263..2268
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /note="hexamer: AATAAA"
     polyA_site      2290
                     /gene="SPC24"
                     /gene_synonym="SPBC24"
                     /note="major polyA site"
ORIGIN      
        1 gtagtcatgg ccgccttccg cgacatagag gaggtgagcc aggggctgct cagcctgctg
       61 ggcgccaacc gcgcggaggc gcagcagcga cggctgctgg ggcgccacga gcaggtggtg
      121 gagcggctgc tggaaacgca agacggtgcc gagaagcagc tgcgagagat cctcaccatg
      181 gagaaggaag tggcccagag ccttctcaat gcgaaggagc aggtgcacca gggaggcgtg
      241 gagctgcagc agctggaagc tgggcttcag gaggctgggg aggaggacac ccgtctgaag
      301 gccagcctcc ttcagctcac cagagagctg gaagagctca aggagattga ggcggatctg
      361 gagcgacagg agaaggaggt cgacgaggac acgacagtca caatcccctc ggccgtgtac
      421 gtggctcaac tttaccacca agttagtaaa attgagtggg attatgagtg tgagccaggg
      481 atggtcaaag gcatccatca tggccccagt gtggcccagc ccatccacct ggacagcacc
      541 cagctctcca ggaaattcat cagcgactac ctctggagtc tggtggacac cgagtggtag
      601 ccaggagcct cgtggctgcg tcttgcaccc agcgggcatc tgccgtggtc agatccattt
      661 caaatgaaat gtggggacat agatcttttt tttttttttt ttgagacaga gtttcactct
      721 tgttgtccag tctggagtgc aatggcacaa tctcagctca cagcaacctc tgcctcctgg
      781 gttcaagcga ttctcctgcc tcagcctcca aagtagctgg gattacaggc atgcgccacc
      841 acacccagct aattttttgt atttggtaga gatggagttt caccatggtg gtcaggctgg
      901 tcttgaactc ctgacctcag gtgatccacc cgcccgcctt ggcctcccaa agtgctagga
      961 ttccaggcgt gagccatcgt gcccagctag acgtcttcct tttttttttt tttttgagac
     1021 ggagtctcac tctgtcgccc aggctggagt gcagtggtgc gatctcagct cactgcaagc
     1081 tccgcctcct cccgggttca cgccattctc ctgcctcagc ctcctgagta gctgggacta
     1141 caggcacctg ccaccatgcg cagctaattt tttgtatttt tagtagagac ggggtttcac
     1201 catgttagac aggatggtct ccatctcctg agtttgtgat ctgcccgcct tggcctccca
     1261 aagtgctggg attacaggtg tgagtcaccg cgcctggcct tttttttttt tttttttact
     1321 ggatttcttt ttctcctgat tttcggcctc agctcctaga gtggcctcag ctaggtaacc
     1381 aacgggagtc gacttatcta tgccaggcct gcagtttcac atctgtgacg ggatgggaca
     1441 aatgacaccg tggtattcga tgacagctcc caggtacttc aaggttggcc cttactcatt
     1501 cagggggtat gtttgagtgc cacggtgtgc ccagactctg ctaggcacca gagagacaag
     1561 atacgagtga catggttctc ctcccactgc tgacggagga attaacacaa aagcagacaa
     1621 cactctagaa ctggagagag gtccagattg cgacaggagc cacaccagag tgtgcaggct
     1681 tcatggagga cagaagcgac ttttgtgccg ggacttaaaa agataaggag gccaggtgtg
     1741 gtggctcatg cctgcaatcc cagcactgta gaaggcagag gccggcagat cacttgagcc
     1801 caggagttgg agaccagact ggggaacatg gcaagacccc atctctacaa aaaaattaaa
     1861 aaatttggcc tggtgtggtg gctcatgcct gtgatcctag cactttggga gttaaaatac
     1921 aacacttccc agctgccctt atagttaagg tctggccagt gaaatgtatg tggaaatgtt
     1981 acatgacact tctagaagga ttcttaaaag acaaatacac cctttcttcc cccttcctat
     2041 ttcctggaat gtgggtgtga tggctggagc cctggcagcc atactgccac gtgtacagac
     2101 gagtgccgtc ctttagggat gacaggagcc tggttccctg atgatggcag agccagatat
     2161 cagcacaaac ttatttaaat gggagagaga aggggagaac caatctcaac ttctaccttg
     2221 tgtaagctgc tggttttggg ggttttctat gacagatacc aaaataaacc ctaactgaac
     2281 atgtactcca
//
