LOCUS       NM_145263               4430 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens spermatogenesis associated 18 (SPATA18), transcript
            variant 1, mRNA.
ACCESSION   NM_145263
VERSION     NM_145263.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ikari,N., Honjo,K., Sagami,Y., Nakamura,Y. and Arakawa,H.
  TITLE     Mieap forms membrane-less organelles involved in cardiolipin
            metabolism
  JOURNAL   iScience 27 (2), 108916 (2024)
   PUBMED   38322995
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Lingui,X., Weifeng,L., Yufei,W. and Yibin,Z.
  TITLE     High SPATA18 Expression and its Diagnostic and Prognostic Value in
            Clear Cell Renal Cell Carcinoma
  JOURNAL   Med Sci Monit 29, e938474 (2023)
   PUBMED   36751118
  REMARK    GeneRIF: High SPATA18 Expression and its Diagnostic and Prognostic
            Value in Clear Cell Renal Cell Carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4430)
  AUTHORS   Sugimura-Nagata,A., Koshino,A., Nagao,K., Nagano,A., Komura,M.,
            Ueki,A., Ebi,M., Ogasawara,N., Tsuzuki,T., Kasai,K., Takahashi,S.,
            Kasugai,K. and Inaguma,S.
  TITLE     SPATA18 Expression Predicts Favorable Clinical Outcome in
            Colorectal Cancer
  JOURNAL   Int J Mol Sci 23 (5), 2753 (2022)
   PUBMED   35269894
  REMARK    GeneRIF: SPATA18 Expression Predicts Favorable Clinical Outcome in
            Colorectal Cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4430)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   5  (bases 1 to 4430)
  AUTHORS   Nakamura,Y., Kitamura,N., Shinogi,D., Yoshida,M., Goda,O.,
            Murai,R., Kamino,H. and Arakawa,H.
  TITLE     BNIP3 and NIX mediate Mieap-induced accumulation of lysosomal
            proteins within mitochondria
  JOURNAL   PLoS One 7 (1), e30767 (2012)
   PUBMED   22292033
  REMARK    GeneRIF: The physical interaction of Mieap, BNIP3 and NIX at the
            mitochondrial outer membrane may play a critical role in the
            translocation of lysosomal proteins from the cytoplasm to the
            mitochondrial matrix.
REFERENCE   6  (bases 1 to 4430)
  AUTHORS   Bornstein,C., Brosh,R., Molchadsky,A., Madar,S., Kogan-Sakin,I.,
            Goldstein,I., Chakravarti,D., Flores,E.R., Goldfinger,N., Sarig,R.
            and Rotter,V.
  TITLE     SPATA18, a spermatogenesis-associated gene, is a novel
            transcriptional target of p53 and p63
  JOURNAL   Mol Cell Biol 31 (8), 1679-1689 (2011)
   PUBMED   21300779
  REMARK    GeneRIF: show that SPATA18 transcription is induced by p53 in a
            variety of cell types of both human and mouse origin
REFERENCE   7  (bases 1 to 4430)
  AUTHORS   Kitamura,N., Nakamura,Y., Miyamoto,Y., Miyamoto,T., Kabu,K.,
            Yoshida,M., Futamura,M., Ichinose,S. and Arakawa,H.
  TITLE     Mieap, a p53-inducible protein, controls mitochondrial quality by
            repairing or eliminating unhealthy mitochondria
  JOURNAL   PLoS One 6 (1), e16060 (2011)
   PUBMED   21264228
  REMARK    GeneRIF: Mieap induced vacuole-like structures (designated as MIV
            for Mieap-induced vacuole), which engulfed and degraded the
            unhealthy mitochondria by accumulating lysosomes
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4430)
  AUTHORS   Miyamoto,Y., Kitamura,N., Nakamura,Y., Futamura,M., Miyamoto,T.,
            Yoshida,M., Ono,M., Ichinose,S. and Arakawa,H.
  TITLE     Possible existence of lysosome-like organella within mitochondria
            and its role in mitochondrial quality control
  JOURNAL   PLoS One 6 (1), e16054 (2011)
   PUBMED   21264221
  REMARK    GeneRIF: Mieap induces intramitochondrial lysosome-like organella
            that plays a critical role in mitochondrial quality control by
            eliminating oxidized mitochondrial proteins
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 4430)
  AUTHORS   Kaindl,A.M., Jakubiczka,S., Lucke,T., Bartsch,O., Weis,J.,
            Stoltenburg-Didinger,G., Aksu,F., Oexle,K., Koehler,K. and
            Huebner,A.
  TITLE     Homozygous microdeletion of chromosome 4q11-q12 causes severe
            limb-girdle muscular dystrophy type 2E with joint hyperlaxity and
            contractures
  JOURNAL   Hum Mutat 26 (3), 279-280 (2005)
   PUBMED   16088906
  REMARK    GeneRIF: beta-sarcoglycan and SPATA18 may have a role in
            limb-girdle muscular dystrophy type 2E
REFERENCE   10 (bases 1 to 4430)
  AUTHORS   Iida,H., Ichinose,J., Kaneko,T., Mori,T. and Shibata,Y.
  TITLE     Complementary DNA cloning of rat spetex-1, a spermatid-expressing
            gene-1, encoding a 63 kDa cytoplasmic protein of elongate
            spermatids
  JOURNAL   Mol Reprod Dev 68 (4), 385-393 (2004)
   PUBMED   15236321
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC093858.2, DC404003.1,
            BC025396.1, BU930386.1, CK904112.1 and AI339078.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_145263.3.
            
            Summary: This gene encodes a p53-inducible protein that is able to
            induce lysosome-like organelles within mitochondria that eliminate
            oxidized mitochondrial proteins, thereby contributing to
            mitochondrial quality control. Dysregulation of mitochondrial
            quality control is associated with cancer and degenerative
            diseases. The encoded protein mediates accumulation of the
            lysosome-like mitochondrial organelles through interaction with B
            cell lymphoma 2 interacting protein 3 and B cell lymphoma 2
            interacting protein 3 like at the outer mitochondrial membrane,
            which allows translocation of lysosomal proteins to the
            mitochondrial matrix from the cytosol. Alternative splicing results
            in multiple transcript variants. [provided by RefSeq, Sep 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC025396.1, SRR1660809.27639.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000295213.9/ ENSP00000295213.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AC093858.2         41306-41332
            28-123              DC404003.1         1-96
            124-3429            BC025396.1         4-3309
            3430-3804           BU930386.1         306-680
            3805-3903           BC025396.1         3685-3783
            3904-4179           CK904112.1         255-530             c
            4180-4430           AI339078.1         3-253               c
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q12"
     gene            1..4430
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /note="spermatogenesis associated 18"
                     /db_xref="GeneID:132671"
                     /db_xref="HGNC:HGNC:29579"
                     /db_xref="MIM:612814"
     exon            1..488
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             402..2018
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mitochondria-eating protein; spermatogenesis associated 18
                     homolog; testis tissue sperm-binding protein Li 71n"
                     /codon_start=1
                     /product="mitochondria-eating protein isoform 1"
                     /protein_id="NP_660306.1"
                     /db_xref="CCDS:CCDS3489.1"
                     /db_xref="GeneID:132671"
                     /db_xref="HGNC:HGNC:29579"
                     /db_xref="MIM:612814"
                     /translation="MAENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELI
                     EQVAKVQGQLFGILTAAAQEGGRNDGVETIKSRLLPWLEASFTAASLGKSVDSKVPSL
                     QDTFDRERHKDPSPRDRDMQQLDSNLNSTRSQCNQVQDDLVETEKNLEESKNRSAISL
                     LAAEEEINQLKKQLKSLQAQEDARHRNTDQRSSENRRSEPWSLEERKREQWNSLKQNA
                     DQQDTEAMSDYKKQLRNLKEEIAVLSAEKSALQGRSSRSRSPSPAPRSRSCSRSRSAS
                     PSTAVKVRRPSPNRSKLSNVARKAALLSRFSDSYSQARLDAQCLLRRCIDKAETVQRI
                     IYIATVEAFHVAKMAFRHFKIHVRKSLTPSYVGSNDFENAVLDYVICHLDLYDSQSSV
                     NDVIRAMNVNPKISFPPVVDFCLLSDFIQEICCIAFAMQALEPPLDIAYGADGEVFND
                     CKYRRSYDSDFTAPLVLYHVWPALMENDCVIMKGEAVTRRGAFWNSVRSVSRCRSRSL
                     SPICPRSQIGLNTMSRSRSPSPIRCGLPRF"
     exon            489..594
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            595..710
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            711..823
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            824..919
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1159
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1421
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1580
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1756
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1757..1880
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..1964
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2010
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2011..4430
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4402..4407
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /note="hexamer: AATAAA"
     polyA_site      4430
                     /gene="SPATA18"
                     /gene_synonym="Mieap; SPETEX1"
                     /note="major polyA site"
ORIGIN      
        1 agtccacccc tggggcgcgc ggctgtcacc cagggcgggg cggcgcgggc gttgccacga
       61 cgcgggccgc gcgcgtccct ggcagccaac ccgtccacgt caaggtttgt ttaataatcg
      121 ccagggtatc tatggccggg ctcaggcggc tgctggggag ccaggagacc gcgcgggacg
      181 gcggatgagg cgcggcggct gcggcccagg gcacctcccc tctggcttcc cgaacccggc
      241 caggtccgac ccgaggggga ggatggaaac acctgccgcg ctctgagccc cccagaagag
      301 aacacccttc ccgccatatc accccacggt cctgcggagg ccaccgcctg gtccccccaa
      361 gtctccatcg cgcagcgtgg ggccgagagg aatagtgagc gatggcggaa aacctgaaaa
      421 gactggtctc aaacgaaact ttacgaacgt tgcaggaaaa gctagacttc tggctgaagg
      481 agtacaacac aaacacgtgt gatcaaaatc taaaccattg ccttgaactc attgagcaag
      541 ttgccaaggt gcagggacaa ctctttggga tcctcacagc agcagcccaa gaaggaggac
      601 gtaatgatgg tgtggaaaca atcaagtcac gccttttgcc ttggctggag gcttccttta
      661 ctgctgcttc cctgggaaaa tctgttgaca gcaaggtccc ctctctgcag gacacgtttg
      721 atagggagag acataaagat cccagtcctc gggatcggga tatgcaacag ttagactcta
      781 atttgaactc aacccggagt caatgcaacc aggttcaaga cgatctggtt gaaactgaaa
      841 agaatcttga agaaagcaag aacagatcgg ccatatccct tttggctgca gaggaggaaa
      901 taaatcagct gaaaaagcag cttaaatctc ttcaagctca ggaggatgcc cgccacagaa
      961 acacagatca gaggagctca gagaataggc ggtcagagcc ttggagcttg gaggagcgga
     1021 agcgtgagca gtggaactca ctcaagcaga atgcagacca gcaggacaca gaagccatgt
     1081 ccgattataa gaaacagctc cgaaacctga aggaggagat agctgttctg tctgctgaga
     1141 aaagtgcact ccaaggaagg tcctccagga gccggtctcc cagccctgcc cctcgcagcc
     1201 gtagctgcag ccgcagcaga tctgccagcc cctccaccgc tgtcaaggtc aggagaccgt
     1261 ccccaaaccg ctccaagctg tccaatgtgg cgcgcaaggc tgccctcttg tcccggttca
     1321 gcgattccta ttcccaggcc cgcctggacg cgcagtgcct gctgcggcgc tgcatcgaca
     1381 aggctgagac cgttcagcgg atcatctaca tcgccacagt ggaggcattc catgtagcaa
     1441 aaatggcatt cagacacttc aagatccatg tgagaaaatc gttgacacca tcttatgtgg
     1501 ggtcgaatga ctttgagaat gctgtcttgg attatgtcat ttgtcatctt gatctatatg
     1561 attctcaaag cagtgtcaat gatgtgatcc gagccatgaa tgtcaatccc aagatttcat
     1621 tccctcctgt cgttgacttt tgccttctca gtgacttcat ccaggagata tgttgcattg
     1681 cctttgcaat gcaggcctta gaaccacccc tagatattgc atatggagca gatggagaag
     1741 tttttaatga ttgcaaatac cgccgcagct acgactcgga tttcactgct cccttagtcc
     1801 tctatcacgt gtggcctgct ctcatggaga atgactgtgt cattatgaag ggagaagctg
     1861 tcaccaggag aggggctttt tggaattcgg tgcgatctgt aagtcgttgt cgaagcagga
     1921 gtttaagtcc catttgcccc cgtagccaaa ttggtttaaa cacgatgtct cgaagtcgga
     1981 gtccttctcc aataagatgt ggattgccaa gattttaaaa gcaccagacc tgctcctttg
     2041 acccagtgcg tggaaacagc tgctttctcc agtgccgcca tctgtcttct gtgtctgcct
     2101 cagacctcac ttaagataat gtcaaaaggc aattctgtgt atcaccccac acagagagtt
     2161 aaatgttttg gcttggcgca tttgtaactt tagatatatt gcattctatt ttattttata
     2221 gatactaatt ccattaattt cataaaaatg attgtatagg catttaggat catattcatt
     2281 cgaagcaaag tccgttacaa aggttcaaga tttccatctc aaaacactac gctcttttat
     2341 gggaactgtg tgaactgaag tggaaagcat ctaccatgct gaggctaaaa gaaaagatga
     2401 atcattttag tttgcagatg gatcgtaaat ataattgttg gtatcagctt tagctcaaaa
     2461 ccaatattag gtgttttaat ttccttttaa ggtttggaag acagccctaa tctcaggttg
     2521 gggagctcat gttagtagca gtgacttaag gctaagtgta gaagataatt taagatacat
     2581 tttctttata tattagccaa caaattatat ttattggttg gcttgctttt ccgttctgat
     2641 tttgagagtg cccagtttgg tttagttgac caatgaatgt caaagctact tagttgagag
     2701 aatttccttg ttcataaatg tagagcagtg atttgattag aagccagctt tgagataaat
     2761 gttaattacc tcatgcatat ctcctgggaa tatttcaaac tgttttaatg catgtgttat
     2821 atataaaagt ttcttgggac atgctcttca cctgttctac ctagttattt gcaaattcag
     2881 acctcctatt gaactctgtc tgaccaaaac tacttaaact caaggcccaa aactaggggc
     2941 accatttact gattttaaat tgagtatata tcccttgact tcttcactgt caaatacttt
     3001 tgaaacttca cgttcaagat aagaatggaa tgttgctttc ttgcaataag taatgttctt
     3061 tctgcctttt tttcactttt aagtcagcct taaacacatg cctcacaaac atctactttc
     3121 tccacatacc tttgagagag acactgaatt ggcctcagct cagttttgca taagcttagt
     3181 gccagaacca gcacctgatg cttttcaggt gaaaataaaa caaacagctt ctctaaagca
     3241 tcttacccct gtgctggagg tttgagggac ctcttcagtg cctgcccctt gagtctaatg
     3301 gtcaccacct cattctgaag tatgagttga attttttgcc ctctttgcat atttacatta
     3361 gtcatcactt tgaagcaatg cagtgtgctg gaaggagcac tatctgccta ggtaactgct
     3421 agtcatgact tggtcatcag cttgctttgt ggcactgagc aagttacttc acttctctga
     3481 gccttggttt tatcataggg tgaggaggtt ggatacaatt agtgcccctc ttaaccctgc
     3541 agactcaatg ttccctttta taacaagtat tttattctga atatgaaatg aaaattaaag
     3601 ttaatataat catctatgtg catgtataat tttaagcagt gaacatagta ccctaactat
     3661 aatataaagc agaaaaaaag gcaactttta ataaaataaa atgtatttca ataaaaaagc
     3721 ttgggtataa ccaccctaga agataaaatt aagtcattag atggctgaac ctgcatgtag
     3781 agccaccagc tacaaatgaa aatcaatgtg tgtattggca acagaaaatc acggtgttgt
     3841 cattggatgt gactttctga agtggtgggc aattcttgtt aatgttttaa acaaaaaaaa
     3901 aaaaacctta cagtcttgcc ctgatttaca cagcagtcac attcctggaa aattcaagtg
     3961 tttattaaaa ctatgcaaca gttactgtgt gttatacgtt gaaaggtctt cactaatatc
     4021 tcactaagta atgagaatgc ctacatatca gaattttttt tttcaggagc caagcacata
     4081 tactgatttg gaaaaaggca caggtagctc agtttatttg ctttctaccc tgcctggcca
     4141 cttgctgttt cttcagtttc taatttgagc tgtaactaca caaggaaagc taaatagtct
     4201 ggaaaatttt tggaaagaat ccacaaagcc aaaggagact ggcctatact cattttatct
     4261 ggggatgtac cttaccctta gagactttga aaaatgtgaa gctcttattt tgtaacctgg
     4321 gtaaatgtta gtttctagat tttcggctta acatctaata ataacattta aaaagtgctt
     4381 ttgtaactat tagttatttg caataaaatg ctttccttct acagtcccaa
//
