LOCUS       NM_001166271            8454 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens spermatogenesis associated 13 (SPATA13), transcript
            variant 1, mRNA.
ACCESSION   NM_001166271
VERSION     NM_001166271.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 8454)
  AUTHORS   Waseem,N.H., Low,S., Shah,A.Z., Avisetti,D., Ostergaard,P.,
            Simpson,M., Niemiec,K.A., Martin-Martin,B., Aldehlawi,H., Usman,S.,
            Lee,P.S., Khawaja,A.P., Ruddle,J.B., Shah,A., Sackey,E., Day,A.,
            Jiang,Y., Swinfield,G., Viswanathan,A., Alfano,G., Chakarova,C.,
            Cordell,H.J., Garway-Heath,D.F., Khaw,P.T., Bhattacharya,S.S.,
            Waseem,A. and Foster,P.J.
  TITLE     Mutations in SPATA13/ASEF2 cause primary angle closure glaucoma
  JOURNAL   PLoS Genet 16 (4), e1008721 (2020)
   PUBMED   32339198
  REMARK    GeneRIF: Mutations in SPATA13 cause primary angle closure glaucoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8454)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 8454)
  AUTHORS   Evans,J.C., Hines,K.M., Forsythe,J.G., Erdogan,B., Shi,M., Hill,S.,
            Rose,K.L., McLean,J.A. and Webb,D.J.
  TITLE     Phosphorylation of serine 106 in Asef2 regulates cell migration and
            adhesion turnover
  JOURNAL   J Proteome Res 13 (7), 3303-3313 (2014)
   PUBMED   24874604
  REMARK    GeneRIF: Phosphorylation of S106 modulates Asef2 guanine nucleotide
            exchange factor activity and Asef2-mediated cell migration and
            adhesion turnover.
REFERENCE   6  (bases 1 to 8454)
  AUTHORS   Bristow,J.M., Sellers,M.H., Majumdar,D., Anderson,B., Hu,L. and
            Webb,D.J.
  TITLE     The Rho-family GEF Asef2 activates Rac to modulate adhesion and
            actin dynamics and thereby regulate cell migration
  JOURNAL   J Cell Sci 122 (Pt 24), 4535-4546 (2009)
   PUBMED   19934221
  REMARK    GeneRIF: Asef2 activates Rac1 to modulate adhesion and actin
            dynamics and thereby regulate cell migration.
REFERENCE   7  (bases 1 to 8454)
  AUTHORS   Sagara,M., Kawasaki,Y., Iemura,S.I., Natsume,T., Takai,Y. and
            Akiyama,T.
  TITLE     Asef2 and Neurabin2 cooperatively regulate actin cytoskeletal
            organization and are involved in HGF-induced cell migration
  JOURNAL   Oncogene 28 (10), 1357-1365 (2009)
   PUBMED   19151759
  REMARK    GeneRIF: Asef2, Neurabin2 and APC cooperatively regulate actin
            cytoskeletal organization and are required for HGF-induced cell
            migration.
REFERENCE   8  (bases 1 to 8454)
  AUTHORS   Lasky-Su,J., Neale,B.M., Franke,B., Anney,R.J., Zhou,K.,
            Maller,J.B., Vasquez,A.A., Chen,W., Asherson,P., Buitelaar,J.,
            Banaschewski,T., Ebstein,R., Gill,M., Miranda,A., Mulas,F.,
            Oades,R.D., Roeyers,H., Rothenberger,A., Sergeant,J.,
            Sonuga-Barke,E., Steinhausen,H.C., Taylor,E., Daly,M., Laird,N.,
            Lange,C. and Faraone,S.V.
  TITLE     Genome-wide association scan of quantitative traits for attention
            deficit hyperactivity disorder identifies novel associations and
            confirms candidate gene associations
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 147B (8), 1345-1354 (2008)
   PUBMED   18821565
REFERENCE   9  (bases 1 to 8454)
  AUTHORS   Kawasaki,Y., Sagara,M., Shibata,Y., Shirouzu,M., Yokoyama,S. and
            Akiyama,T.
  TITLE     Identification and characterization of Asef2, a guanine-nucleotide
            exchange factor specific for Rac1 and Cdc42
  JOURNAL   Oncogene 26 (55), 7620-7267 (2007)
   PUBMED   17599059
  REMARK    GeneRIF: These results suggest that similar to Asef, Asef2 plays an
            important role in cell migration, and that Asef2 activated by
            truncated mutant APC is required for aberrant migration of
            colorectal tumor cells.
REFERENCE   10 (bases 1 to 8454)
  AUTHORS   Hamann,M.J., Lubking,C.M., Luchini,D.N. and Billadeau,D.D.
  TITLE     Asef2 functions as a Cdc42 exchange factor and is stimulated by the
            release of an autoinhibitory module from a concealed C-terminal
            activation element
  JOURNAL   Mol Cell Biol 27 (4), 1380-1393 (2007)
   PUBMED   17145773
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP201334.1, AK092754.1, AL359736.19, DB221004.1 and AK055770.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001166271.2.
            
            Transcript Variant: This variant (1) encodes isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: BX648244.1, SRR1803614.140528.1
                                    [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA1968540, SAMEA1968968 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000382108.8/ ENSP00000371542.3
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-137               BP201334.1         5-141
            138-545             AK092754.1         136-543
            546-1633            AL359736.19        68331-69418
            1634-2193           DB221004.1         1-560
            2194-5295           AK055770.1         402-3503
            5296-8454           AL359736.19        149091-152249
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q12.12"
     gene            1..8454
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /note="spermatogenesis associated 13"
                     /db_xref="GeneID:221178"
                     /db_xref="HGNC:HGNC:23222"
                     /db_xref="MIM:613324"
     exon            1..213
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            214..1977
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             325..4158
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     spermatogenesis-associated protein 13; adenomatous
                     polyposis coli stimulated exchange factor 2;
                     APC-stimulated guanine nucleotide exchange factor 2"
                     /codon_start=1
                     /product="spermatogenesis-associated protein 13 isoform 1"
                     /protein_id="NP_001159743.1"
                     /db_xref="CCDS:CCDS53857.1"
                     /db_xref="GeneID:221178"
                     /db_xref="HGNC:HGNC:23222"
                     /db_xref="MIM:613324"
                     /translation="MTQAAVRPWAPCLENMTTAPNGLGPGPAAPCAGSDLKDAKMVTS
                     LACGNGVCGCSPGGDTDTQEAKLSPAKLVRLFSTSRKRTGAHPERPHSMVLVGNSSTW
                     NTLASFRKMGSFKKLKSSVLKGIQSREGSNACSKGEASEHGLGKSIPNGAVPGAQASR
                     GSPLAPGPACGALRPAEWGTLDGSDLEDTDDAFQRSTHRSRSLRRAYGLGRICLLDAP
                     QNHATPTIATGQVPAVCEILVRDPENNSMGYRRSKSTDNLAFLKKSSFKRKSTSNLAD
                     LRTAHDARVPQRTLSSSSTDSQKLGSGRTKRWRSPIRAKDFDRVFKLVSNVTEAAWRR
                     ESPRSGAPSPGEASLRLQAHSRLHDDYSRRVSRSTEQDSRRGGAVMHGTTATCTVAPG
                     FGSATSKGPHLDADTAVFPLETKSSWAVESDSSCTCSSLPSPIVQDVLSKDSCDPNAG
                     SQLTFDPEQPPTPLRPTTPKPQSPQSPQSPGAGSASCHSNHSALSANSEESEGRAEEP
                     AQREPGPVSLQDPLEATHGDEGSKDLLVNIGVAAGPEEKEKEEVVPDGPWRRSSSQDE
                     ERTEAQRTPKRRWGSGRRPRPRPFSDYGQLASRSLSIPEDSVAADPQKEDRVDEDPQA
                     SMTSASPEDQNAPVGCPKGARRRRPISVIGGVSLYGTNQTEELDNLLTQPASRPPMPA
                     HQVPPYKAVSARFRPFTFSQSTPIGLDRVGRRRQMRASNVSSDGGTEPSALVDDNGSE
                     EDFSYEDLCQASPRYLQPGGEQLAINELISDGNVVCAEALWDHVTMDDQELGFKAGDV
                     IQVLEASNKDWWWGRSEDKEAWFPASFVRLRVNQEELSENSSSTPSEEQDEEASQSRH
                     RHCENKQQMRTNVIREIMDTERVYIKHLRDICEGYIRQCRKHTGMFTVAQLATIFGNI
                     EDIYKFQRKFLKDLEKQYNKEEPHLSEIGSCFLQNQEGFAIYSEYCNNHPGACLELAN
                     LMKQGKYRHFFEACRLLQQMIDIAIDGFLLTPVQKICKYPLQLAELLKYTTQEHGDYS
                     NIKAAYEAMKNVACLINERKRKLESIDKIARWQVSIVGWEGLDILDRSSELIHSGELT
                     KITKQGKSQQRTFFLFDHQLVSCKKDLLRRDMLYYKGRLDMDEMELVDLGDGRDKDCN
                     LSVKNAFKLVSRTTDEVYLFCAKKQEDKARWLQACADERRRVQEDKEMGMEISENQKK
                     LAMLNAQKAGHGKSKGYNRCPVAPPHQGLHPIHQRHITMPTSVPQQQVFGLAEPKRKS
                     SLFWHTFNRLTPFRK"
     exon            1978..2343
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2488
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2625
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2626..2805
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..2991
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2992..3171
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3172..3404
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3405..3534
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3535..3907
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3908..3982
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3983..8454
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8435..8440
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /note="hexamer: ATTAAA"
     polyA_site      8454
                     /gene="SPATA13"
                     /gene_synonym="ARHGEF29; ASEF2"
                     /note="major polyA site"
ORIGIN      
        1 gagtgtgctg ccgcggcgcg ggaggagagt gtgcgttgcg ctttctcccg cgatcgccct
       61 gccggtcgct agctccgagg gcgcgcactg gatcccaggc tcctccgaga gtgcggcgac
      121 ctcggggctc cgccggcacc ggaggtggct ctgagggcag ggacgtgttg gacacgctga
      181 ctttgtaggc tccgccaaga ggcgccgcag gagcccgtgg ccacccagga gcccgatgtg
      241 caaggcaggt gtgagtgcca ggacggcatt cctggagatg aaggcctgga gctgcggtct
      301 gcggactcgg cagtgcccgt ggccatgacc caggctgccg tgcggccctg ggcaccctgc
      361 ctggagaaca tgaccactgc cccaaacggc ctcgggccag gccccgcagc cccctgtgca
      421 ggctcggacc tgaaagacgc caagatggtg acctcccttg cgtgtggaaa tggagtctgt
      481 ggctgcagcc ctggtggcga cacggacacc caggaagcca aactcagccc agccaagctt
      541 gtgcgcctct tttccaccag tcggaagagg acgggtgccc accccgagcg gccccactcc
      601 atggtcctgg tggggaactc ctccacatgg aacaccctcg cctccttccg gaaaatggga
      661 tcctttaaga aactgaagtc ctcagtcctg aaaggaattc agagccgaga ggggtcaaat
      721 gcctgttcaa agggagaggc ttcggagcat ggcctgggaa agtccatccc aaatggcgct
      781 gtcccaggag cccaggcaag caggggctcc cccttagcac cgggaccagc atgtggtgcc
      841 ctcaggccag cagagtgggg cacattggat ggctccgacc tcgaggacac ggacgatgcc
      901 ttccagcgga gcacacaccg ctcccgcagc ctccgcagag cctacggcct gggccgcatc
      961 tgcctgctgg atgcgcccca gaaccatgcg acacccacga tagccactgg ccaggtgccc
     1021 gccgtgtgtg agattctcgt gagggaccct gaaaacaaca gcatgggcta caggaggagc
     1081 aagagcacgg acaatcttgc ctttctgaag aagagctcct ttaagcggaa gtccacctcc
     1141 aatcttgcag acctcaggac ggcccatgac gcacgggtac cacagaggac cctgagcagt
     1201 tcctccactg actcccaaaa gcttgggtca ggaaggacca aacgctggag gagcccgata
     1261 agggccaagg actttgacag agtcttcaaa cttgtgagca atgtgactga ggctgcctgg
     1321 aggagggaga gtcctaggag tggggcccca tcccctggag aggccagcct gagacttcag
     1381 gcacacagcc ggctgcatga cgactactcc cgccgcgtct ccaggagcac tgagcaggac
     1441 agcaggcggg gcggggcggt catgcatggg accactgcaa cctgcaccgt ggcccccggt
     1501 ttcggctcag ccacctctaa ggggccccac ctagacgctg acactgccgt atttcctctt
     1561 gaaaccaaaa gttcctgggc ggtggaaagc gacagttcct gcacttgcag ctctttgcca
     1621 agcccgattg tccaggatgt gttgagcaaa gactcctgtg acccaaacgc tggcagccag
     1681 ttgacatttg accctgagca gcctcccacc cctctaaggc ccaccacacc caagccccag
     1741 agccctcaga gcccccagag ccccggggca ggaagtgcca gctgtcacag caatcacagt
     1801 gccctgtccg cgaattcaga ggaaagtgaa ggaagggcag aagagcctgc tcagagagag
     1861 ccagggcctg tgtccttgca ggatcccctg gaagccacac atggtgatga gggcagcaag
     1921 gaccttctgg tgaacattgg tgtggcagcc ggcccagaag aaaaggagaa ggaggaggtc
     1981 gtccctgatg gcccctggag gcgaagctca tcacaggatg aggaaaggac agaggcacag
     2041 agaaccccca agaggagatg gggctctggg agacggccaa ggcctcggcc attctctgac
     2101 tacggccagc tggccagccg cagtttgtct attcctgaag actcggttgc tgcagacccc
     2161 cagaaggaag accgtgtgga cgaggacccc caggcaagca tgacttctgc cagccctgaa
     2221 gaccagaatg ctccagtggg ctgccccaaa ggagcccgga gaaggcgccc catttccgtg
     2281 ataggtgggg tcagcttgta tgggaccaac cagacggagg aactggacaa tcttctgacc
     2341 caaccggctt ccaggccgcc catgcctgct caccaggtgc caccctacaa ggctgtgtcg
     2401 gcccggttcc ggcccttcac attctcccag agcaccccca ttgggttgga ccgtgtggga
     2461 cgccggcggc agatgagagc atccaacgtt tcttcagatg gaggtactga gccctctgcc
     2521 ttagtggatg acaacggtag tgaggaggac ttcagctatg aagacctctg ccaggccagc
     2581 cctcggtacc tgcagcccgg cggggagcag ctggccatca atgagctgat cagtgatggc
     2641 aacgtggtct gcgcagaagc cctgtgggac catgtgacca tggatgacca ggaactgggc
     2701 ttcaaagccg gggatgtcat ccaggttctg gaagcctcca acaaggactg gtggtggggc
     2761 cgcagtgaag ataaggaagc ctggttcccc gcgagcttcg tcagattgcg agtgaatcag
     2821 gaagagctgt cggaaaactc cagcagcacc cccagtgagg agcaggacga ggaggccagc
     2881 cagagccgcc acagacactg tgagaacaag cagcagatgc ggaccaacgt catccgggag
     2941 atcatggaca ccgagcgggt gtacatcaaa cacctcaggg acatctgtga gggctatatc
     3001 cgacagtgcc gcaagcacac aggaatgttc accgttgcgc agctagccac tatttttgga
     3061 aacattgaag atatttacaa attccaaaga aagtttctga aagaccttga gaaacagtac
     3121 aacaaagagg aacctcactt aagtgaaata ggatcttgct ttcttcaaaa tcaagagggc
     3181 tttgccatct attccgagta ctgcaacaac cacccgggcg cctgcctgga gctcgccaac
     3241 ctcatgaagc agggcaagta cagacatttc tttgaagcct gccgcctgct gcagcagatg
     3301 attgacatcg ccatcgacgg gttcctgctc acaccagtgc agaagatctg caaatacccg
     3361 ctgcagctgg ccgagctgct caagtatacc acacaggaac acggtgatta cagcaacata
     3421 aaggcagcat atgaggccat gaagaatgtg gcctgtctga tcaacgagcg caagcgcaag
     3481 ctggagagca tcgacaagat agctcgctgg caggtgtcta tcgtgggctg ggagggactg
     3541 gatatcttag accgaagctc agaattgatt cattctgggg agctgaccaa aatcactaag
     3601 caaggcaaaa gccagcagcg gacgttcttc ctgtttgacc accagctggt gtcctgcaag
     3661 aaggacctgc tgcgcaggga catgctgtac tacaagggcc ggctggacat ggatgagatg
     3721 gagcttgtgg acctggggga tgggcgcgac aaggactgca acctcagcgt gaaaaatgcc
     3781 ttcaagctcg tcagtaggac cacagacgag gtttatttgt tttgtgccaa aaaacaagaa
     3841 gacaaggcga ggtggctgca ggcctgtgca gatgaaagga ggcgggtgca agaggacaag
     3901 gagatgggaa tggaaatttc agaaaaccag aagaaacttg ccatgttaaa tgctcaaaag
     3961 gcaggacatg gaaagtcaaa aggctacaac aggtgccctg tggccccacc gcaccagggc
     4021 ctgcacccca tccaccagcg ccacatcact atgcccacaa gcgtccccca gcagcaggtc
     4081 tttggcctgg cggaacccaa gaggaagtcc tcgctcttct ggcacacctt caacaggctc
     4141 acccccttcc ggaaatgaaa acaggaggct gtgcttccat ggagctgggt gtcaagagaa
     4201 gaactgtctt tgtttcttgt gtgcttcaat ccagggaaag tttcttggac ccagtgataa
     4261 aaacttcctt ttagggatca atgaaggaga gaaggtcttg gaatcacctt cagtctttgg
     4321 agacccagct gcctttgtgg aagggaggag acggtcatga cacaaagctt tatcctacac
     4381 agaaacaccc gtgacccact atgagatggc ccagatgtgg gacccggtac catgctctaa
     4441 agcgagtgat taggcagcag ctgaagccac ccctgctgat gatgagcaag tgcctgctgc
     4501 aggtccaaac acagcatcca gggctttgca gttcctaagg agtgatgagg ttagaggatc
     4561 acttctgcat ttgattttca aggatgccgt caagacgggg ttgacacaat gctgcacgtg
     4621 tctggtcaca cttagaaatt gagctcttac tctcttctgt aatactgggg gacctacagc
     4681 tgccgtgggg ctgaccacgg tgttccctgg catcgtctgt gtccacacag atgctaactg
     4741 gtagtgcaaa tgtcatcctg caaggttcct cttcctgcaa gcaaagtgga gagaaagaag
     4801 atgcatctgt caccttcatc agggtcctca gtgcagagca acttacgcat cctcaagaat
     4861 ccactgcttt tcaggcaagg agggagaaat cctgctgcac actggctttg tcccggagtc
     4921 ggattccctc ctgcctgcac gccttcagta actccgagca gaaatcacat cttgcccaca
     4981 tgctgtaacc taagaaactg ctatgcaagg ctgggtgctg tggctcatgc ctgtaatccc
     5041 agcactttgg gaggccaagg caggtggatc acctgaggtc aggagttcga gacaagcgtg
     5101 gccaatatgg caaagcccag tctctactaa taatacaaaa attagctggg catggtggcg
     5161 catgcctgta atcccagcta ctgggaaggc tgaggtagga gaatcttttg aatctgggaa
     5221 gcggaggttg cagtgagctg agatcgcacc actgcactcc agcctgggag atacagcgag
     5281 actgtctcca aaaacaaaaa caaaaacaaa aaacattcaa acaactgttt gcaatataaa
     5341 aagctcattt actgtaatat ttatgataca gtgaatatga aaatgcactg gtcagaaggc
     5401 actctcaaag agccgcactg ctcctgacat cgtccttagc aatgaagtca caaagacagc
     5461 caaagcagtc ctgcttcttg gaaatcagaa gctgccttta tcacatataa agccaaacag
     5521 ggcataacca tgtcacgtga gcatgtcatc aggcttctga ggacttgttc tttataaaaa
     5581 aagaccttca caaaatatct tggcttagag atagcagtct ttattaacaa aggccaccta
     5641 ggctgacacc tgcagataat catctccttt tctttgtcta tgttgtacat tttcatgata
     5701 taacttttaa ctatgtctag agaaggcagg ctctgcaaga gaggtgccct ttcaacccgc
     5761 tcagtgccct ggacaggaga tgctgtgtta aactgttaat ggatatctat atgagaagct
     5821 catttttgta tgctatccct gcagtttttt tttttctaac aggcccatgt ttgagaataa
     5881 acaagtctgt gatgtcagag acaaaggtgt attcttcagt ctgcaggtgt gtggcacctc
     5941 ccttctcccc tgcagccccc cacatccaga gccgttcctg agagtgacat catgcatcaa
     6001 gaaaacataa ccttggtcct caggtgaacc cttggaacat tctgtgaccg cctgatgtcc
     6061 attctgagcc accttggcac acatgcttac aggcagcact gctaagggtt caggtgcccc
     6121 atggctgaca gcccgagttg cttctgtgga ccatcatgcc gctcggcacg tcctgagaca
     6181 gaagttgctg caggaaggag cttctggaga ggtcctgtgg catgtgtggg ggtgtgtgtg
     6241 tgtatgtttc cttcttgaac agacattcca actttagatg tgtttataga actgaccttt
     6301 ttactaacaa aatacaatga tatatgttgg aaactactta atatgctttt cctgcacacc
     6361 ttagcaataa ctgtaggggt ctctgctaga gttgtttgta tgtacagcaa ttttgaacaa
     6421 attgttttaa atgtaatata agagaattag tttaaggaag taaagagaat catttgcttg
     6481 tgttacattt tcagtgagga ttcagtttaa gagtcattct taggacttcc atttcctaat
     6541 atttattcat gggtaatgaa gaaatggttt gcattttgtg gccagtccta atttattttc
     6601 cagctgagcc ctaacttccg gctcccacct acctccacgg acttcctaac agagacttat
     6661 gaataccagg atgtgttttt gttaagtcag gttcaattcg ttgcccctgt cagttttata
     6721 gagtgtgagg gtcactccat taaagatctc tcctgggtgg atcctacttg gatgttcagg
     6781 tgattttgaa aactgctaac atttttaaaa ggctagaaca tcctttgact tcttgaaaat
     6841 ctgcatgtct ggcttgggtt ttattaccac atgcctgagt tcttcaagaa tggaaggctc
     6901 aagtattctc atcttccatt tgccaaactt ccttcctgat ttgagtcacg tgttccactt
     6961 ggaaagaaag ggaacagaga gcctcctcca tggacagtgt atgaatttca ttgggaatct
     7021 tgctctctcc cgcctctatg cctttctctc tttttaacct tactttacat aatattatag
     7081 atgggccaag aaaagaaaag atgacataac attttgatga atttcaccta ttccattctt
     7141 cacgtttcag aattggtcga ctttgttaga agataattga agtagccttg ggtcaaaagc
     7201 aaccttttca attgtgatca tacctaaaac atataaaaac cctgccgtag attaaaagca
     7261 attataaaat cataaaattg aatgtttgca gaatcctgga gcagtagatt tctttgtctt
     7321 tggcctgcgg actagaaaga gggcagcagt agtatgctgg agcttccctg ggataccagc
     7381 cacatggttt cttttcatta gatctgattt ttgtttccca ctgtagatct gattttgtag
     7441 ttgaaaacat ttcaccacca tcaaacacta tttctgaata ttgtgccttt ttatacctag
     7501 cctagatgaa aaccgatgcc attcttattc agaaaatccc cccatcctac atgactgtta
     7561 tctagacata aagcaaagtg catttaattc aaaatttggt tcacaatata agtattttgt
     7621 aaaagccagc tgaaccagca ttttatcagg tggaaatctc tgcaagccaa attgctgata
     7681 ctccttcatg cagatcaact tggtgtccca gtcagaatag aacagcataa ttacctggag
     7741 ttagggggag tatttctgca ctattacttg tcagggagag aagaaactta gaattgtccc
     7801 tcaaaggagt gtcaagaagt atgaataaat gtcctttcac cagctcacag gccagaaatg
     7861 gaggacccaa gtcaactagg tgaaactact agcagaccca gctttcccat aataacctaa
     7921 tctgcaaatt gttctattaa agtctcattg ttttcaggat gcaatgaaag tggatttcaa
     7981 aaggctttgg aaaaataagt ggaacatgac tgatcttgaa aaaaaaagca aaagcttaaa
     8041 tatttgatac aagtttactt agctacaaca tactttacat tgttgccttt agttatctca
     8101 caggcactga cattttatat ttagaaaata cttttaatct ttctaatctt tttttgtaaa
     8161 tattagtgtc cattctgtat gactcgctaa cctactttgc aaggctttgg gcaacatttt
     8221 agctcattaa cttcaagatg atgtgtcatc tgtataggtc aaagaatggg acttctgaac
     8281 tgaggaattt gctgttgaca gccaaagtat agtgtacaag attgatgtaa cttgatatgt
     8341 atttttgttg aagttttttg taaaaaaaaa ttatttacaa tgttatttga atgatttttt
     8401 taaatgctgt gaatctatat ttgttgtttt gtatattaaa attcatttgc caaa
//
