LOCUS       NM_032461                466 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens SPANX family member B1 (SPANXB1), mRNA.
ACCESSION   NM_032461 NM_139019 XM_001128409 XM_003846318
VERSION     NM_032461.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 466)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 466)
  AUTHORS   Kannan,A., Philley,J.V., Hertweck,K.L., Ndetan,H., Singh,K.P.,
            Sivakumar,S., Wells,R.B., Vadlamudi,R.K. and Dasgupta,S.
  TITLE     Cancer Testis Antigen Promotes Triple Negative Breast Cancer
            Metastasis and is Traceable in the Circulating Extracellular
            Vesicles
  JOURNAL   Sci Rep 9 (1), 11632 (2019)
   PUBMED   31406142
  REMARK    GeneRIF: Cancer Testis Antigen Promotes Triple Negative Breast
            Cancer Metastasis and is Traceable in the Circulating Extracellular
            Vesicles.
            Erratum:[Sci Rep. 2020 Jan 31;10(1):1907. doi:
            10.1038/s41598-020-58045-z.. PMID: 32005891]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 466)
  AUTHORS   Yilmaz-Ozcan,S., Sade,A., Kucukkaraduman,B., Kaygusuz,Y.,
            Senses,K.M., Banerjee,S. and Gure,A.O.
  TITLE     Epigenetic mechanisms underlying the dynamic expression of
            cancer-testis genes, PAGE2, -2B and SPANX-B, during
            mesenchymal-to-epithelial transition
  JOURNAL   PLoS One 9 (9), e107905 (2014)
   PUBMED   25229454
  REMARK    GeneRIF: Epigenetic mechanisms underlying the dynamic expression of
            cancer-testis genes, PAGE2, -2B and SPANX-B, during
            mesenchymal-to-epithelial transition
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 466)
  AUTHORS   Kouprina,N., Pavlicek,A., Noskov,V.N., Solomon,G., Otstot,J.,
            Isaacs,W., Carpten,J.D., Trent,J.M., Schleutker,J., Barrett,J.C.,
            Jurka,J. and Larionov,V.
  TITLE     Dynamic structure of the SPANX gene cluster mapped to the prostate
            cancer susceptibility locus HPCX at Xq27
  JOURNAL   Genome Res 15 (11), 1477-1486 (2005)
   PUBMED   16251457
REFERENCE   6  (bases 1 to 466)
  AUTHORS   Westbrook,V.A., Schoppee,P.D., Diekman,A.B., Klotz,K.L.,
            Allietta,M., Hogan,K.T., Slingluff,C.L., Patterson,J.W.,
            Frierson,H.F., Irvin,W.P. Jr., Flickinger,C.J., Coppola,M.A. and
            Herr,J.C.
  TITLE     Genomic organization, incidence, and localization of the SPAN-x
            family of cancer-testis antigens in melanoma tumors and cell lines
  JOURNAL   Clin Cancer Res 10 (1 Pt 1), 101-112 (2004)
   PUBMED   14734458
REFERENCE   7  (bases 1 to 466)
  AUTHORS   Zendman,A.J., Zschocke,J., van Kraats,A.A., de Wit,N.J.,
            Kurpisz,M., Weidle,U.H., Ruiter,D.J., Weiss,E.H. and van
            Muijen,G.N.
  TITLE     The human SPANX multigene family: genomic organization, alignment
            and expression in male germ cells and tumor cell lines
  JOURNAL   Gene 309 (2), 125-133 (2003)
   PUBMED   12758128
REFERENCE   8  (bases 1 to 466)
  AUTHORS   Wang,Z., Zhang,Y., Liu,H., Salati,E., Chiriva-Internati,M. and
            Lim,S.H.
  TITLE     Gene expression and immunologic consequence of SPAN-Xb in myeloma
            and other hematologic malignancies
  JOURNAL   Blood 101 (3), 955-960 (2003)
   PUBMED   12393489
  REMARK    GeneRIF: correlation between SPAN-Xb gene expression and B-cell
            immune responses in myeloma and other hematologic malignancies
REFERENCE   9  (bases 1 to 466)
  AUTHORS   Westbrook,V.A., Diekman,A.B., Naaby-Hansen,S., Coonrod,S.A.,
            Klotz,K.L., Thomas,T.S., Norton,E.J., Flickinger,C.J. and Herr,J.C.
  TITLE     Differential nuclear localization of the cancer/testis-associated
            protein, SPAN-X/CTp11, in transfected cells and in 50% of human
            spermatozoa
  JOURNAL   Biol Reprod 64 (1), 345-358 (2001)
   PUBMED   11133693
REFERENCE   10 (bases 1 to 466)
  AUTHORS   Westbrook,V.A., Diekman,A.B., Klotz,K.L., Khole,V.V., von
            Kap-Herr,C., Golden,W.L., Eddy,R.L., Shows,T.B., Stoler,M.H.,
            Lee,C.Y., Flickinger,C.J. and Herr,J.C.
  TITLE     Spermatid-specific expression of the novel X-linked gene product
            SPAN-X localized to the nucleus of human spermatozoa
  JOURNAL   Biol Reprod 63 (2), 469-481 (2000)
   PUBMED   10906052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ457796.1.
            
            On Nov 22, 2018 this sequence version replaced NM_032461.3.
            
            Summary: Temporally regulated transcription and translation of
            several testis-specific genes is required to initiate the series of
            molecular and morphological changes in the male germ cell lineage
            necessary for the formation of mature spermatozoa. This gene is a
            member of the SPANX family of cancer/testis-associated genes, which
            are located in a cluster on chromosome X. The SPANX genes encode
            differentially expressed testis-specific proteins that localize to
            various subcellular compartments. This particular family member
            contains an additional 18 nucleotides in its coding region compared
            to the other family members in the same gene cluster. This family
            member is also subject to gene copy number variation. Although the
            protein encoded by this gene contains consensus nuclear
            localization signals, the major site for subcellular localization
            of expressed protein is in the cytoplasmic droplets of ejaculated
            spermatozoa. This protein provides a biochemical marker for
            studying the unique structures in spermatazoa, while attempting to
            further define its role in spermatogenesis. [provided by RefSeq,
            Apr 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF098307.1, AJ457796.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000449283.2/ ENSP00000405202.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-466               AJ457796.1         4-469
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq27.1"
     gene            1..466
                     /gene="SPANXB1"
                     /gene_synonym="B1; CT11.2; SPANX-B; SPANXB; SPANXB2;
                     SPANXF1; SPANXF2"
                     /note="SPANX family member B1"
                     /db_xref="GeneID:728695"
                     /db_xref="HGNC:HGNC:14329"
                     /db_xref="MIM:300669"
     exon            1..190
                     /gene="SPANXB1"
                     /gene_synonym="B1; CT11.2; SPANX-B; SPANXB; SPANXB2;
                     SPANXF1; SPANXF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..412
                     /gene="SPANXB1"
                     /gene_synonym="B1; CT11.2; SPANX-B; SPANXB; SPANXB2;
                     SPANXF1; SPANXF2"
                     /note="cancer/testis antigen family 11, member 2; sperm
                     protein associated with the nucleus, X chromosome, family
                     member B1; nuclear-associated protein SPAN-Xb; SPANX
                     family member F1; sperm protein associated with the
                     nucleus on the X chromosome B/F; cancer/testis antigen
                     11.2; SPANX family member B/F; nuclear-associated protein
                     SPAN-Xf; SPANX family, member B2; SPANX family, member F2"
                     /codon_start=1
                     /product="sperm protein associated with the nucleus on the
                     X chromosome B1"
                     /protein_id="NP_115850.2"
                     /db_xref="CCDS:CCDS44006.1"
                     /db_xref="GeneID:728695"
                     /db_xref="HGNC:HGNC:14329"
                     /db_xref="MIM:300669"
                     /translation="MGQQSSVRRLKRSVPCESNEANEANEANKTMPETPTGDSDPQPA
                     PKKMKTSESSTILVVRYRRNVKRTSPEELLNDHARENRINPDQMEEEEFIEITTERPK
                     K"
     exon            191..466
                     /gene="SPANXB1"
                     /gene_synonym="B1; CT11.2; SPANX-B; SPANXB; SPANXB2;
                     SPANXF1; SPANXF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      447..452
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPANXB1"
                     /gene_synonym="B1; CT11.2; SPANX-B; SPANXB; SPANXB2;
                     SPANXF1; SPANXF2"
                     /note="hexamer: AATAAA"
     polyA_site      466
                     /gene="SPANXB1"
                     /gene_synonym="B1; CT11.2; SPANX-B; SPANXB; SPANXB2;
                     SPANXF1; SPANXF2"
                     /note="major polyA site"
ORIGIN      
        1 accaggaggg tatgcatagg gagggcaaga gctctgggcc actgcgaaga ttcaaaagct
       61 ccaaaaacct actgtagaca tcgaagaacc aatatataca atgggccaac aatccagtgt
      121 ccgcaggctg aagaggagcg tcccctgtga atccaacgag gccaacgagg ccaatgaggc
      181 caacaagacg atgccggaga ccccaactgg ggactcagac ccgcaacctg ctcctaaaaa
      241 aatgaaaaca tctgagtcct cgaccatact agtggttcgc tacaggagga acgtgaaaag
      301 aacatctcca gaggaactgc tgaatgacca cgcccgagag aacagaatca accccgacca
      361 aatggaggag gaggaattca tagaaataac gactgaaaga cctaaaaagt agcaagaagc
      421 tacatccctc aaacttcggc aatgaaaata aagtttgaga agctga
//
