LOCUS       NM_145662                408 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens SPANX family member A2 (SPANXA2), mRNA.
ACCESSION   NM_145662 XM_001128442
VERSION     NM_145662.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Li,J., Bo,H., Zhu,F., Li,Q., Chen,T., Lei,S. and Fan,L.
  TITLE     Hypomethylated SPANXA1/A2 promotes the metastasis of head and neck
            squamous cell carcinoma
  JOURNAL   Med Oncol 37 (12), 112 (2020)
   PUBMED   33175201
  REMARK    GeneRIF: Hypomethylated SPANXA1/A2 promotes the metastasis of head
            and neck squamous cell carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 408)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   4  (bases 1 to 408)
  AUTHORS   Hansen,S., Eichler,E.E., Fullerton,S.M. and Carrell,D.
  TITLE     SPANX gene variation in fertile and infertile males
  JOURNAL   Syst Biol Reprod Med 55, 18-26 (2010)
   PUBMED   20073942
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   5  (bases 1 to 408)
  AUTHORS   Westbrook,V.A., Schoppee,P.D., Vanage,G.R., Klotz,K.L.,
            Diekman,A.B., Flickinger,C.J., Coppola,M.A. and Herr,J.C.
  TITLE     Hominoid-specific SPANXA/D genes demonstrate differential
            expression in individuals and protein localization to a distinct
            nuclear envelope domain during spermatid morphogenesis
  JOURNAL   Mol Hum Reprod 12 (11), 703-716 (2006)
   PUBMED   17012309
REFERENCE   6  (bases 1 to 408)
  AUTHORS   Kouprina,N., Mullokandov,M., Rogozin,I.B., Collins,N.K.,
            Solomon,G., Otstot,J., Risinger,J.I., Koonin,E.V., Barrett,J.C. and
            Larionov,V.
  TITLE     The SPANX gene family of cancer/testis-specific antigens: rapid
            evolution and amplification in African great apes and hominids
  JOURNAL   Proc Natl Acad Sci U S A 101 (9), 3077-3082 (2004)
   PUBMED   14973187
REFERENCE   7  (bases 1 to 408)
  AUTHORS   Westbrook,V.A., Schoppee,P.D., Diekman,A.B., Klotz,K.L.,
            Allietta,M., Hogan,K.T., Slingluff,C.L., Patterson,J.W.,
            Frierson,H.F., Irvin,W.P. Jr., Flickinger,C.J., Coppola,M.A. and
            Herr,J.C.
  TITLE     Genomic organization, incidence, and localization of the SPAN-x
            family of cancer-testis antigens in melanoma tumors and cell lines
  JOURNAL   Clin Cancer Res 10 (1 Pt 1), 101-112 (2004)
   PUBMED   14734458
REFERENCE   8  (bases 1 to 408)
  AUTHORS   Zendman,A.J., Zschocke,J., van Kraats,A.A., de Wit,N.J.,
            Kurpisz,M., Weidle,U.H., Ruiter,D.J., Weiss,E.H. and van
            Muijen,G.N.
  TITLE     The human SPANX multigene family: genomic organization, alignment
            and expression in male germ cells and tumor cell lines
  JOURNAL   Gene 309 (2), 125-133 (2003)
   PUBMED   12758128
REFERENCE   9  (bases 1 to 408)
  AUTHORS   Westbrook,V.A., Diekman,A.B., Naaby-Hansen,S., Coonrod,S.A.,
            Klotz,K.L., Thomas,T.S., Norton,E.J., Flickinger,C.J. and Herr,J.C.
  TITLE     Differential nuclear localization of the cancer/testis-associated
            protein, SPAN-X/CTp11, in transfected cells and in 50% of human
            spermatozoa
  JOURNAL   Biol Reprod 64 (1), 345-358 (2001)
   PUBMED   11133693
REFERENCE   10 (bases 1 to 408)
  AUTHORS   Westbrook,V.A., Diekman,A.B., Klotz,K.L., Khole,V.V., von
            Kap-Herr,C., Golden,W.L., Eddy,R.L., Shows,T.B., Stoler,M.H.,
            Lee,C.Y., Flickinger,C.J. and Herr,J.C.
  TITLE     Spermatid-specific expression of the novel X-linked gene product
            SPAN-X localized to the nucleus of human spermatozoa
  JOURNAL   Biol Reprod 63 (2), 469-481 (2000)
   PUBMED   10906052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB110020.1.
            
            On Nov 22, 2018 this sequence version replaced NM_145662.3.
            
            Summary: Temporally regulated transcription and translation of
            several testis-specific genes is required to initiate the series of
            molecular and morphological changes in the male germ cell lineage
            necessary for the formation of mature spermatozoa. This gene is a
            member of the SPANX family of cancer/testis-associated genes, which
            are located in a cluster on chromosome X. The SPANX genes encode
            differentially expressed testis-specific proteins that localize to
            various subcellular compartments. This particular gene maps to
            chromosome X in a head-to-head orientation with SPANX family member
            A1 and appears to be a duplication of that locus. The protein
            encoded by this gene targets to the nucleus where it associates
            with nuclear vacuoles and the redundant nuclear envelope. Based on
            its association with these poorly characterized regions of the
            sperm nucleus, this protein provides a biochemical marker to study
            unique structures in spermatazoa while attempting to further define
            its role in spermatogenesis. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CB113891.1, CB122914.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370518.4/ ENSP00000359549.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-408               CB110020.1         1-408
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq27.2"
     gene            1..408
                     /gene="SPANXA2"
                     /gene_synonym="CT11.1; CT11.3; SPANX; SPANX-A; SPANX-C;
                     SPANXA; SPANXC"
                     /note="SPANX family member A2"
                     /db_xref="GeneID:728712"
                     /db_xref="HGNC:HGNC:14328"
                     /db_xref="MIM:300493"
     exon            1..132
                     /gene="SPANXA2"
                     /gene_synonym="CT11.1; CT11.3; SPANX; SPANX-A; SPANX-C;
                     SPANXA; SPANXC"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..354
                     /gene="SPANXA2"
                     /gene_synonym="CT11.1; CT11.3; SPANX; SPANX-A; SPANX-C;
                     SPANXA; SPANXC"
                     /note="sperm protein associated with the nucleus, X
                     chromosome, family member A2; nuclear-associated protein
                     SPAN-Xa; SPANX family member A; cancer/testis antigen
                     11.1; Sperm protein associated with the nucleus on the X
                     chromosome C; Cancer/testis antigen 11.3;
                     Cancer/testis-associated protein CTp11; Nuclear-associated
                     protein SPAN-Xc"
                     /codon_start=1
                     /product="sperm protein associated with the nucleus on the
                     X chromosome A"
                     /protein_id="NP_663695.1"
                     /db_xref="CCDS:CCDS44007.1"
                     /db_xref="GeneID:728712"
                     /db_xref="HGNC:HGNC:14328"
                     /db_xref="MIM:300493"
                     /translation="MDKQSSAGGVKRSVPCDSNEANEMMPETPTGDSDPQPAPKKMKT
                     SESSTILVVRYRRNFKRTSPEELLNDHARENRINPLQMEEEEFMEIMVEIPAK"
     exon            133..408
                     /gene="SPANXA2"
                     /gene_synonym="CT11.1; CT11.3; SPANX; SPANX-A; SPANX-C;
                     SPANXA; SPANXC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      389..394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPANXA2"
                     /gene_synonym="CT11.1; CT11.3; SPANX; SPANX-A; SPANX-C;
                     SPANXA; SPANXC"
                     /note="hexamer: AATAAA"
     polyA_site      408
                     /gene="SPANXA2"
                     /gene_synonym="CT11.1; CT11.3; SPANX; SPANX-A; SPANX-C;
                     SPANXA; SPANXC"
                     /note="major polyA site"
ORIGIN      
        1 actgagaaga ttcaaaacct acaaaagcct gccactgaca ttgaagaacc aatatataca
       61 atggacaaac aatccagtgc cggcggggtg aagaggagcg tcccctgtga ttccaacgag
      121 gccaacgaga tgatgccgga gaccccaact ggggactcag acccgcaacc tgctcctaaa
      181 aaaatgaaaa catctgagtc ctcgaccata ctagtggttc gctacaggag gaactttaaa
      241 agaacatctc cagaggaact gctgaatgac cacgcccgag agaacagaat caaccccctc
      301 caaatggagg aggaggaatt catggaaata atggttgaaa tacctgcaaa gtagcaagaa
      361 gctacatctc tcaaccttgg gcaatgaaaa taaagtttga gaagctga
//
